Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| fungi associated with storage rots of cocoyams (colocasia spp.) in nsukka, nigeria. | cocoyam (colocasia spp.) corms and cormels showing spoilage symptoms were collected from many stores in nsukka locality and examined for rot and associated fungal pathogens. aspergillus niger, botryodiplodia theobromae, corticium rolfsii, geotrichum candidum, fusarium oxysporum, and f. solani were recovered from rotten cocoyams. the representative isolates of these species caused cocoyam rot in pathogenicity tests. the rot due to a. niger, b. theobromae and c. rolfsii was extensive resulting in ... | 1996 | 20882465 |
| [antimicrobic and hemolytic activity of low-molecular metabolits of brown seaweed laminaria cichorioides miyabe]. | antimicrobial and hemolytic activity of ethanol extract of brown seaweed laminaria cichorioides miyabe, its lipophilic fractions, various classes of substances of lipophilic fraction, such as chlorophylls, fucoxanthin, monogalactosyldiacylglycerols, digalactosyldiacylglycerols, sulfoquinovosyldiacylglycerols, and fatty acids were investigated. the antimicrobial activity was studied by means of yeast cells safale s-04 and candida albicans cmm 455, fungi aspergillus niger cmm 4634 and fusarium oxy ... | 2010 | 20873173 |
| growth and mycotoxin production by fungi in atmospheres containing 80% carbon dioxide and 20% oxygen. | the effect of atmosphere containing 80% co(2) and 20% o(2) on growth of mucor plumbeus, fusarium oxysporum, byssochlamys fulva, byssochlamys nivea, penicillium commune, penicillium roqueforti, aspergillus flavus, eurotium chevalieri and xeromyces bisporus was investigated. production of aflatoxin by a. flavus, patulin by b. nivea, roquefortine c by p. roqueforti, and cyclopiazonic acid by p. commune was also studied. fungal growth was evaluated by three methods: colony diameter, hyphal length or ... | 2010 | 20864200 |
| [advances in past five years in study on traditional chinese medicine against fungi]. | prevalence of fungal infection increased rapidly in recent years. the organisms developed resistance to conventional antifungal agents such as azoles and polyenes, while the traditional chinese medicine (tcm) showed certain advantages in the fight against fungal infections. this paper reviewed the latest research progress of tcm against common pathogenic fungi such as candida albicans, dermatophytes, aspergillus, malassezia, fusarium oxysporum fungus, sporothrix schenckii, cryptococcus neoformna ... | 2010 | 20862976 |
| [isolation and identification of endophytic fungi from different swollen root of rehmannia glutinosa]. | the swollen root of rehmannia glutinosa is used as one kind of important chinese traditional medicine. the root of r. glutinosa usually swelled in rotational cropping but not in continuous cropping. the rhizosphere microorganisms of r. glutinosa under different farming condition were thought related to that. in this study, the endophytic fungi in the root of r. glutinosa growing in various soil conditions were isolated for the study of the relationship between the microorganisms and the root enl ... | 2010 | 20862955 |
| permeabilization of fungal hyphae by the plant defensin nad1 occurs through a cell wall-dependent process. | the antifungal activity of the plant defensin nad1 involves specific interaction with the fungal cell wall, followed by permeabilization of the plasma membrane and entry of nad1 into the cytoplasm. prior to this study, the role of membrane permeabilization in the activity of nad1, as well as the relevance of cell wall binding, had not been investigated. to address this, the permeabilization of fusarium oxysporum f. sp. vasinfectum hyphae by nad1 was investigated and compared with that by other a ... | 2010 | 20861017 |
| the molecular pathogenicity of fusarium keratitis: a fungal transcriptional regulator promotes hyphal penetration of the cornea. | the pathogenic mechanisms of fungal infection during human keratomycosis were investigated in an ex vivo corneal model that used strains of fusarium oxysporum differing in the production of a fungal transcription factor. | 2010 | 20856109 |
| first report of an anti-tumor, anti-fungal, anti-yeast and anti-bacterial hemolysin from albizia lebbeck seeds. | a monomeric 5.5-kda protein with hemolytic activity toward rabbit erythrocytes was isolated from seeds of albizia lebbeck by using a protocol that involved ion-exchange chromatography on q-sepharose and sp-sepharose, hydrophobic interaction chromatography on phenyl-sepharose, and gel filtration on superdex 75. it was unadsorbed on both q-sepharose and sp-sepharose, but adsorbed on phenyl-sepharose. its hemolytic activity was fully preserved in the ph range 0-14 and in the temperature range 0-100 ... | 2011 | 20850957 |
| effect of polyacetylenic acids from prunella vulgaris on various plant pathogens. | this study is aiming at characterizing antifungal substances from the methanol extract of prunella vulgaris and at investigating those substances' antifungal and antioomycete activities against various plant pathogens. | 2010 | 20849392 |
| the characterization of transaldolase gene tal from pichia stipitis and its heterologous expression in fusarium oxysporum. | the 972 bp length of transaldolase gene tal was cloned from pichia stipitis cicc1960, encoding a 323 amino acid protein with a calculated molecular mass of 35.36 kda and isoelectric point of 5.20. real time pcr analysis demonstrated that the mrna transcript level of constitutive tal gene rise on xylose, glucose, fructose, mannose, galactose and sucrose as carbon source, respectively. furthermore, the transcription of tal gene in p. stipitis on xylose was higher than on other carbon source, indic ... | 2011 | 20845075 |
| effect of biofumigation with manure amendments and repeated biosolarization on fusarium densities in pepper crops. | in the region of murcia (southeast spain), sweet pepper has been grown as a monoculture in greenhouses for many years. until 2005, when it was banned, soils were disinfested with methyl bromide (mb) to control pathogens and to prevent soil fatigue effects. the genus fusarium plays an important role in the microbiological component associated with yield decline in pepper monocultures. in the present study, soils were treated with manure amendments, alone (biofumigation, b) or in combination with ... | 2011 | 20820866 |
| fungal microbiota from rain water and pathogenicity of fusarium species isolated from atmospheric dust and rainfall dust. | in order to determine the presence of fusarium spp. in atmospheric dust and rainfall dust, samples were collected during september 2007, and july, august, and october 2008. the results reveal the prevalence of airborne fusarium species coming from the atmosphere of the south east coast of spain. five different fusarium species were isolated from the settling dust: fusarium oxysporum, f. solani, f. equiseti, f. dimerum, and f. proliferatum. moreover, rainwater samples were obtained during signifi ... | 2011 | 20820862 |
| impact of biological control agents on fusaric acid secreted from fusarium oxysporum f. sp. gladioli (massey) snyder and hansen in gladiolus grandiflorus corms. | fusaric acid (fa) (5-n-butylpuridine 2-carboxyl acid), a highly toxic secondary metabolite produced by fusarium oxysporum strains, plays a significant role in disease development. the abilities of three f. oxysporum f. sp. gladioli (massey) snyder and hansen isolates (g010; 649-91; and 160-57) to produce fa in infected gladiolus corm tissues was evaluated in vitro in relation to the presence of two biological control agents, trichoderma harzianum t22, and aneurinobacillus migulanus. pathogenicit ... | 2011 | 20814727 |
| phenazine-1-carboxylic acid is a more important contributor to biocontrol fusarium oxysporum than pyrrolnitrin in pseudomonas fluorescens strain psd. | phenazines and pyrrolnitrin (prn) are broad spectrum antibiotics, produced by bacteria, more so by the biocontrol strains to kill the phytopathogens in soil. we have studied a rhizospheric soil isolate of pseudomonas fluorescens strain psd producing both phenazine-1-carboxylic acid (pca) and prn. in order to study the contribution of these antibiotics, the phzd and prnc genes involved in pca and prn biosynthesis, were disrupted in a site-specific manner using a group ii intron-based targetron ge ... | 2011 | 20813512 |
| allelochemical effects of volatile compounds and organic extracts from muscodor yucatanensis, a tropical endophytic fungus from bursera simaruba. | muscodor yucatanensis, an endophytic fungus, was isolated from the leaves of bursera simaruba (burseraceae) in a dry, semideciduous tropical forest in the ecological reserve el eden, quintana roo, mexico. we tested the mixture of volatile organic compounds (vocs) produced by m. yucatanensis for allelochemical effects against other endophytic fungi, phytopathogenic fungi and fungoids, and plants. vocs were lethal to guignardia mangifera, colletotrichum sp., phomopsis sp., alternaria solani, rhizo ... | 2010 | 20809145 |
| cordymin, an antifungal peptide from the medicinal fungus cordyceps militaris. | cordymin, an antifungal peptide with a molecular mass of 10,906 da and an n-terminal amino acid sequence distinct from those of previously reported proteins, was purified from the medicinal mushroom cordyceps militaris. the isolation protocol comprised ion exchange chromatography of the aqueous extract on sp-sepharose and mono s and gel filtration on superdex 75 by a fast protein liquid chromatography system. cordymin was adsorbed on both cation exchangers. the peptide inhibited mycelial growth ... | 2011 | 20739167 |
| antifungal activities of some indole derivatives. | nine indole derivatives were evaluated in vitro against fusarium graminearum, alternaria alternata, helminthosporium sorokinianum, pyricularia oryzae, fusarium oxysporum f. sp. vasinfectum, fusarium oxysporum f. sp. cucumarinum, and alternaria brassicae. most of the compounds were found to possess antifungal activities. especially compounds 2, 5, 8, and 9 exhibited broad-spectrum antifungal activities against the above-mentioned seven phytopathogenic fungi, and showed more potent activities than ... | 2010 | 20737910 |
| synthesis and antifungal activities of some 2,6-bis-(un)substituted phenoxymethylpyridines. | several 2,6-bis-(un)substituted phenoxymethylpyridines were synthesized and evaluated in vitro against fusarium graminearum, helminthosporium sorokinianum, alternaria brassicae, alternaria alternata, and fusarium oxysporum f. sp. vasinfectum. among all derivatives, compound 3a exhibited a broad-spectrum antifungal activity against the five phytopathogenic fungi. | 2010 | 20737909 |
| allelochemical tricin in rice hull and its aurone isomer against rice seedling rot disease. | one promising area of rice disease management is the potential of exploiting biological control agents. rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding. rice hull has been potentially used to control paddy weeds, but little information is available on rice disease. this study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull, and t ... | 2010 | 20730995 |
| an antifungal defensin from phaseolus vulgaris cv. 'cloud bean'. | an antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kda was purified from dried seeds of phaseolus vulgaris 'cloud bean'. the isolation procedure entailed anion exchange chromatography on deae-cellulose, affinity chromatography an affi-gel blue gel, cation exchange chromatography on sp-sepharose, and gel filtration by fast protein liquid chromatography on superdex 75. although the antifungal peptide was unadsorbed on deae-cellulose, it was adsorbed on both a ... | 2011 | 20729048 |
| acaconin, a chitinase-like antifungal protein with cytotoxic and anti-hiv-1 reverse transcriptase activities from acacia confusa seeds. | from the seeds of acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward rhizoctonia solani with an ic₅₀ of 30±4 µm was isolated. acaconin demonstrated an n-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kda. it was isolated by chromatography on q-sepharose, sp-sepharose and superdex 75 and was not bound by either ion exchanger. acaconin was devoid of chitinase activity. the antifungal activit ... | 2010 | 20725649 |
| a proteomics approach to study synergistic and antagonistic interactions of the fungal-bacterial consortium fusarium oxysporum wild-type msa 35. | fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic f. oxysporum strains. in this study, f. oxysporum wild-type (wt) msa 35, a biocontrol multispecies consortium that consists of a fungus and numerous rhizobacteria mainly belonging to gamma-proteobacteria, was analyzed by two complementary metaproteomic approaches (2-de co ... | 2010 | 20707000 |
| transport and fate of methyl iodide and its pest control in soils. | for fumigants, information on transport and fate as well as pest control is needed to develop management practices with the fewest negative environmental effects while offering sufficient pest control efficacy. for this purpose, a 2-d soil chamber with a surface-mounted flux chamber was designed to determine volatilization, real-time soil gas-phase concentration, degradation, and organism survivability after methyl iodide (mei) fumigation. three types of pests were used to give a broad spectrum ... | 2010 | 20704226 |
| ecological risk of long-term chlorimuron-ethyl application to soil microbial community: an in situ investigation in a continuously cropped soybean field in northeast china. | chlorimuron-ethyl has been widely used for the soybean production of china, but less information is available on the possible risk of long-term application of this herbicide. | 2011 | 20700659 |
| genetic and functional diversity among the antagonistic potential fluorescent pseudomonads isolated from tea rhizosphere. | twenty-five fluorescent pseudomonads from rhizospheric soil of six tea gardens in four district of upper assam, india were isolated and screened for antagonistic activity against fungal pathogens such as fusarium oxysporum f. sp. raphani (for), fusarium oxysporum f. sp. ciceri (foc), fusarium semitectum (fs), and rhizoctonia solani (rs); and bacterial pathogens-staphylococcus aureus (sa), escherichia coli (ec), and klebsiella pneumoniae (kp). most of the isolates exhibited strong antagonistic ac ... | 2011 | 20689953 |
| inactivation of food-borne spoilage and pathogenic micro-organisms on the surface of a photoactive polymer. | the photodynamic action of a novel photoactive polymer comprising covalently bound anthraquinone (aq) moieties was evaluated after developing a methodology to reliably immobilize viable micro-organisms onto polymer film surfaces. the survival of escherichia coli, bacillus cereus (vegetative cells and spores), fusarium oxysporum and saccharomyces cerevisiae microbes inoculated on the surface of inert polymeric substrates was assessed to determine the effect of inoculum composition, drying rate an ... | 2010 | 20670362 |
| antifungal activity of glyceollins isolated from soybean elicited with aspergillus sojae. | the aim of this study was to examine the efficacy of glyceollins isolated from soybean seeds elicited with aspergillus sojae for its antifungal potential. glyceollins i, ii, and iii were determined by hplc-ms analysis. the glyceollins (200 and 600 microg/disk) revealed remarkable antifungal effect against fusarium oxysporum , phytophthora capsici, sclerotinia sclerotiorum, and botrytis cinerea, within the growth inhibition range of 10.9-61.0%, along with their respective mic values ranging from ... | 2010 | 20666365 |
| development of chickpea near-isogenic lines for fusarium wilt. | four pairs of near-isogenic lines (nils) of chickpea with resistance/susceptibility to fusarium oxysporum f. sp. ciceris (foc) have been developed in this study. these lines were produced by searching in advanced recombinant inbred lines (rils) that are segregating for foc race 5 based on a phenotypic screening. the sequence tagged microsatellite (stms) marker ta59, closely linked to wilt resistance genes on linkage group 2 (lg2) of the chickpea map, was used to assist the selection of resistant ... | 2010 | 20652529 |
| a nitrogen response pathway regulates virulence functions in fusarium oxysporum via the protein kinase tor and the bzip protein meab. | during infection, fungal pathogens activate virulence mechanisms, such as host adhesion, penetration and invasive growth. in the vascular wilt fungus fusarium oxysporum, the mitogen-activated protein kinase fmk1 is required for plant infection and controls processes such as cellophane penetration, vegetative hyphal fusion, or root adhesion. here, we show that these virulence-related functions are repressed by the preferred nitrogen source ammonium and restored by treatment with l-methionine sulf ... | 2010 | 20639450 |
| rapid detection of aspergillus flavus in rice using biofunctionalized carbon nanotube field effect transistors. | in the present study, we have used carbon nanotube field effect transistors (fet) that have been functionalized with protein g and igg to detect aspergillus flavus in contaminated milled rice. the adsorbed protein g on the carbon nanotubes walls enables the igg anti-aspergillus antibodies to be well oriented and therefore to display full antigen binding capacity for fungal antigens. a solution of tween 20 and gelatine was used as an effective blocking agent to prevent the non-specific binding of ... | 2011 | 20632162 |
| brevibacillus laterosporus strain bpm3, a potential biocontrol agent isolated from a natural hot water spring of assam, india. | a bacterial strain designated as bpm3 isolated from mud of a natural hot water spring of nambar wild life sanctuary, assam, india, strongly inhibited growth of phytopathogenic fungi (fusarium oxysporum f. sp. ciceri, f. semitectum, magnaporthe grisea and rhizoctonia oryzae) and gram-positive bacterium (staphylococcus aureus). the maximum growth and antagonistic activity was recorded at 30°c, ph 8.5 when starch and peptone were amended as carbon and nitrogen sources, respectively. in greenhouse e ... | 2011 | 20630733 |
| structural, spectral and biological studies of binuclear tetradentate metal complexes of n3o schiff base ligand synthesized from 4,6-diacetylresorcinol and diethylenetriamine. | the binuclear schiff base, h2l, ligand was synthesized by reaction of 4,6-diacetylresorcinol with diethylenetriamine in the molar ratio 1:2. the coordination behavior of the h2l towards cu(ii), ni(ii), co(ii), zn(ii), fe(iii), cr(iii), vo(iv) and uo2(vi) ions has been investigated. the elemental analyses, magnetic moments, thermal studies and ir, electronic, 1h nmr, esr and mass spectra were used to characterize the isolated ligand and its metal complexes. the ligand acts as dibasic with two n3o ... | 2010 | 20627808 |
| production of antimicrobial silver nanoparticles in water extracts of the fungus amylomyces rouxii strain ksu-09. | a fungal strain, ksu-09, isolated from the roots of date palm (phoenix dactylifera), was identified as amylomyces rouxii based on sequence analysis of the internal transcribed spacer (its) region of its rrna genes. mycelia-free water extracts obtained from mycelium suspended in water for 72h facilitated the production of stable, predominantly monodispersed and spherical silver nanoparticles (agnps) in the size range of 5-27nm upon addition of 1mm silver nitrate, as determined by the xrd, afm and ... | 2010 | 20619641 |
| chitin synthase-deficient mutant of fusarium oxysporum elicits tomato plant defence response and protects against wild-type infection. | a mutant of the root pathogen fusarium oxysporum f. sp. lycopersici, deficient in class v chitin synthase, has been shown previously to be nonvirulent. in this study, we tested the hypothesis that the cause of its avirulence could be the elicitation of the induced plant defence response, leading to the restriction of fungal infection. co-inoculation of tomato plants with the wild-type strain and the deltachsv mutant resulted in a significant reduction in symptom development, supporting a protect ... | 2010 | 20618706 |
| synthesis, characterization, and antifungal activity of novel quaternary chitosan derivatives. | three novel quaternary chitosan derivatives were successfully synthesized by reaction of chloracetyl chitosan (cacs) with pyridine (pacs), 4-(5-chloro-2-hydroxybenzylideneamino)-pyridine (chpacs), and 4-(5-bromo-2-hydroxybenzylideneamino)-pyridine (bhpacs). the chemical structure of the prepared chitosan derivatives was confirmed by fourier transform infrared (ft-ir) and (13)c nuclear magnetic resonance ((13)c nmr) and their antifungal activity against cladosporium cucumerinum, monilinia fructic ... | 2010 | 20615498 |
| synthesis and antimicrobial activity of some new thiazole, thiophene and pyrazole derivatives containing benzothiazole moiety. | in an attempt to find a new class of antimicrobial agents, a series of thiazole, thiophene, pyrazole and other related products containing benzothiazole moiety were prepared via the reaction of n-(benzothiazol-2-yl)-2-cyanoacetamide (1) with appropriate chemical reagents. these compounds were screened for their antibacterial activity against gram-positive bacteria (staphylococcus aureus and streptococcus pyogenes), gram-negative bacteria (pseudomonas phaseolicola and pseudomonas fluorescens) and ... | 2010 | 20605657 |
| a novel case of fusarium oxysporum infection in an atlantic bottlenose dolphin (tursiops truncatus). | a necropsy was performed on a captive-born, 10-yr-old male atlantic bottlenose dolphin (tursiops truncatus) after it died acutely. gross necropsy findings revealed hemorrhage within the right cerebrum, right cerebellum, and right eye. histopathologic findings revealed a moderate multifocal acute necrotizing meningoencephalitis with intralesional fungal hyphae. several pieces of cerebrum and cerebellum and cerebrospinal fluid were sent to the fungus testing laboratory in san antonio, texas (u.s.a ... | 2010 | 20597220 |
| cellular response of fusarium oxysporum to crocidolite asbestos as revealed by a combined proteomic approach. | cellular mechanisms of asbestos toxicity rely, at least in part, on the chemical composition of these minerals. iron ions are directly involved in the accepted mechanism of fiber toxicity because they constitute active centers where release of free radicals and reactive oxygen species takes place. although no current technology is available for the remediation of asbestos polluted sites, the soil fungus fusarium oxysporum was found to be very effective in iron extraction from crocidolite asbesto ... | 2010 | 20578744 |
| [pathogen identification of pinellia ternata tuber disease and selection of fungicide]. | the rotten tuber of pinellia ternata was found as an important disease during the growing season in tianshui production area. the isolated pathogens were tested following koch's postulates and identified as fusarium oxysporum. the suitable growth conditions for the f. oxysporum were 15-30 degrees c, ph 6-8, the optimal condition was 21.9 degrees c and ph 7.2. some fungicides were demonstrated to be effective to inhibit the pathogen growth. 70% thiophanate-methyl and 58% metalaxyl mz were most ef ... | 2010 | 20575381 |
| conventional and microwave assisted synthesis of some new n-[(4-oxo-2-substituted aryl -1, 3-thiazolidine)-acetamidyl]-5-nitroindazoles and its antimicrobial activity. | several new n-[(4-oxo-2-substituted aryl-1, 3-thiazolidine)-acetamidyl]-5-nitroindazoles (4a-l) were synthesized from n-(arylidene amino acetamidyl)-5-nitroindazoles (3a-l). the reactions were carried out by both conventional as well as microwave method. the structures of these compounds were confirmed by ir, (1)h nmr, (13)c nmr, fab-mass spectra and also by microanalytical data. the newly synthesized compounds were evaluated for their antimicrobial activity against variety of bacterial and fung ... | 2010 | 20570024 |
| fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. | summary taxonomy: vascular wilt fungus; ascomycete although sexual stage is yet to be found. the most closely related teleomorphic group, gibberella, is classified within the pyrenomycetes. host range: very broad at the species level. more than 120 different formae speciales have been identified based on specificity to host species belonging to a wide range of plant families. disease symptoms: initial symptoms of vascular wilt include vein clearing and leaf epinasty, followed by stunting, yellow ... | 2003 | 20569392 |
| nitrogen-responsive genes are differentially regulated in planta during fusarium oxyspsorum f. sp. lycopersici infection. | summary nitrogen is an essential growth component whose availability will limit microbial spread, and as such it serves as a key control point in dictating an organism's adaptation to various environments. little is known about fungal nutrition in planta. to enhance our understanding of this process we examined the transcriptional adaptation of fusarium oxysporum f. sp. lycopersici, the causal agent for vascular wilt in tomato, during nutritional stress and plant colonization. using rt-pcr and m ... | 2005 | 20565671 |
| stable integration and expression of wasabi defensin gene in "egusi" melon (colocynthis citrullus l.) confers resistance to fusarium wilt and alternaria leaf spot. | production of "egusi" melon (colocynthis citrullus l.) in west africa is limited by fungal diseases, such as alternaria leaf spot and fusarium wilt. in order to engineer "egusi" resistant to these diseases, cotyledonary explants of two "egusi" genotypes, 'ejagham' and nhc1-130, were transformed with agrobacterium tumefaciens strain eha101 harbouring wasabi defensin gene (isolated from wasabia japonica l.) in a binary vector pekh1. after co-cultivation for 3 days, infected explants were transferr ... | 2010 | 20552202 |
| nuclear dynamics during germination, conidiation, and hyphal fusion of fusarium oxysporum. | in many fungal pathogens, infection is initiated by conidial germination. subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (gfp)- and cherry fluorescent protein (chfp)-labeled nuclei in the plant pathogen fusarium oxysporum. hyphae of f. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical comp ... | 2010 | 20543061 |
| antifungal activity of eugenol against penicillium, aspergillus, and fusarium species. | the antifungal activity of eugenol in a model system against aspergilli (aspergillus niger, aspergillus terreus, and emericella nidulans), penicilli (penicillium expansum, penicillium glabrum, and penicillium italicum), and fusaria (fusarium oxysporum and fusarium avenaceum) was investigated. minimum detection time (time to attain a colony diameter of 1 cm) and the kinetic parameters were evaluated. the effectiveness of the active compound seemed to be strain or genus dependent; 100 mg/liter rep ... | 2010 | 20537272 |
| haemolymph protein and lipid profile of rhipicephalus (boophilus) microplus infected by fungi. | the current study evaluates the protein and lipid profile of haemolymph of rhipicephalus (boophilus) microplus engorged females infected by metarhizium anisopliae, beauveria bassiana or fusarium oxysporum. ticks were immersed or inoculated with conidial suspension. haemolymph was collected from the dorsal surface of engorged females. the results showed altered total protein amounts; however, no significant difference was observed on electrophoretic profile among haemolymph samples. in addition, ... | 2010 | 20537114 |
| trichoderma harzianum and glomus intraradices modify the hormone disruption induced by fusarium oxysporum infection in melon plants. | the plant hormones salicylic acid (sa), jasmonic acid (ja), ethylene (et), and abscisic acid (aba) are known to play crucial roles in plant disease and pest resistance. changes in the concentrations of these plant hormones in melon plant shoots, as a consequence of the interaction between the plant, the pathogen fusarium oxysporum, the antagonistic microorganism trichoderma harzianum, and the arbuscular mycorrhizal fungus glomus intraradices were investigated. attack by f. oxysporum activated a ... | 2010 | 20528186 |
| cu(ii): a "signaling molecule" of the mangrove endophyte fusarium oxysporum zzf51? | we previously reported the isolation of cu-fusaric acid (cu-fa) complex from the mangrove endophyte fusarium oxysporum zzf51. in this study, we explored the mechanism of cu-fa production in the strain zzf51 by comparing with that of another endophyte fusarium sp. b2, which produced fa but not cu-fa in the same culture condition. the results allowed us to hypothesize that cu(2+) may act as a "signaling molecule" to awaken the silent fa biosynthetic genes in zzf51, inducing intracellular productio ... | 2010 | 20526798 |
| cloning, heterologous expression, and functional characterization of a chitinase gene, lbchi32, from limonium bicolor. | in the present study, an endochitinase gene, lbchi32, was cloned from limonium bicolor. the cdna sequence of lbchi32 was 1,443 bp in length and encoded 319 amino acid residues. the dna sequence of lbchi32 was 2,512 bp in length and contained three exons and two introns. the lbchi32 gene was inserted into a ppic9 vector and transferred into pichia pastoris strains gs115 and km71 for heterologous expression. sds-page analyses indicated that lbchi32 was expressed in both gs115 and km71 and that it ... | 2010 | 20512617 |
| tolerance in banana to fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots. | summary fusarium wilt, caused by the fungal pathogen fusarium oxysporum f. sp. cubense (foc), is one of the most destructive diseases of bananas. in the tropics and subtropics, cavendish banana varieties are highly susceptible to foc race 4 (vcg 0120). cavendish selection gctcv-218 was shown to have significantly lower disease severity and incidence compared with susceptible cultivar williams in replicated greenhouse and field trials. suppression subtractive hybridization (ssh) was previously ca ... | 2007 | 20507503 |
| the mixed xylem sap proteome of fusarium oxysporum-infected tomato plants. | summary secreted proteins are known to play decisive roles in plant-fungus interactions. to study the molecular details of the interaction between the xylem-colonizing, plant-pathogenic fungus fusarium oxysporum and tomato, the composition of the xylem sap proteome of infected tomato plants was investigated and compared with that of healthy plants. two-dimensional gel separation and mass spectrometry yielded peptide masses and peptide sequences of 33 different proteins. despite the absence of co ... | 2007 | 20507493 |
| functional analysis of nlp genes from botrytis elliptica. | summary we functionally analysed two nep1-like protein (nlp) genes from botrytis elliptica (a specialized pathogen of lily), encoding proteins homologous to the necrosis and ethylene-inducing protein (nep1) from fusarium oxysporum. single gene replacement mutants were made for benep1 and benep2, providing the first example of transformation and successful targeted mutagenesis in this fungus. the virulence of both mutants on lily leaves was not affected. benep1 and benep2 were individually expres ... | 2007 | 20507492 |
| trifluralin herbicide-induced resistance of melon to fusarium wilt involves expression of stress- and defence-related genes. | summary to identify genes involved in trifluralin herbicide-induced resistance of melon to fusarium oxysporum f. sp. melonis, suppression subtractive hybridization (ssh) and cdna-amplified fragment-length polymorphism (cdna-aflp) were used. a total of 123 clones-60 of which have never been isolated from melon-were isolated, sequenced and annotated. a significant proportion (35%) of the total 123 clones exhibited similarity to genes that have been formerly described as stress- or defence-related. ... | 2007 | 20507475 |
| the global nitrogen regulator, fnr1, regulates fungal nutrition-genes and fitness during fusarium oxysporum pathogenesis. | summary fusarium oxysporum is a soil-borne pathogen that infects plants through the roots and uses the vascular system for host ingress. specialized for this route of infection, f. oxysporum is able to adapt to the scarce nutrient environment in the xylem vessels. here we report the cloning of the f. oxysporum global nitrogen regulator, fnr1, and show that it is one of the determinants for fungal fitness during in planta growth. the fnr1 gene has a single conserved gata-type zinc finger domain a ... | 2006 | 20507463 |
| fusarium wilt (fusarium oxysporum f. sp. vasinfectum) genes expressed during infection of cotton (gossypium hirsutum)dagger. | summary we sought to identify fusarium oxysporum f. sp. vasinfectum (fov) genes that may be associated with pathogenicity. initially we utilized microarray and q-pcr technology to identify fov genes expressed in root and hypocotyl tissues during a compatible infection of cotton. we identified 218 fungal clones representing 174 fov non-redundant genes as expressed in planta. the majority of the expressed sequences were expressed in infected roots, with only six genes detected in hypocotyl tissue. ... | 2006 | 20507430 |
| pathogenicity of fusarium oxysporum against the larvae of culex quinquefasciatus (say) and anopheles stephensi (liston) in laboratory. | the entomopathogenic fungi fusarium oxysporum are the next generation mosquito controlling agent. f. oxysporum basically contains unique toxin and can be a selectively good agent in tropical countries. we are reporting here the efficacy of the metabolites of f. oxysporum against the larvae of anopheles stephensi and culex quinquefasciatus in the laboratory. f. oxysporum was grown on czapek dox broth. the bioassays were run at five different concentrations (1.30, 1.60, 1.77, 1.90, and 2.00 ppm). ... | 2010 | 20499096 |
| morphological changes of fusarium oxysporum induced by cf66i, an antifungal compound from burkholderia cepacia. | the morphological effects of cf66i, an antifungal compound produced by burkholderia cepacia, on growing hyphae of fusarium oxysporum were studied by fluorescence microscopy (fm) and transmission electron microscopy (tem). at 20 μg/ml, cf66i strongly inhibited growth and induced significant changes of the hyphal morphology. these changes included swelling of hyphae with considerable thickening cell wall and abnormal chitin deposition, which was indicative of the alterations in cell wall structure ... | 2010 | 20495943 |
| identification of a hypothetical protein from podospora anserina as a nitroalkane oxidase. | the flavoprotein nitroalkane oxidase (nao) from fusarium oxysporum catalyzes the oxidation of primary and secondary nitroalkanes to their respective aldehydes and ketones. structurally, the enzyme is a member of the acyl-coa dehydrogenase superfamily. to date no enzymes other than that from f. oxysporum have been annotated as naos. to identify additional potential naos, the available database was searched for enzymes in which the active site residues asp402, arg409, and ser276 were conserved. of ... | 2010 | 20481475 |
| development of the molecular methods for rapid detection and differentiation of fusarium oxysporum and f. oxysporum f. sp. niveum in taiwan. | fusarium wilt, caused by fusarium oxysporum (fo), is one of the most important fungal diseases worldwide. like other plant pathogens, fo displays specialized forms in association with its hosts. for example, f. oxysporum f. sp. niveum (fon) is the damaging pathogen causing fusarium wilt disease on watermelon, whereas f. oxysporum f. sp. cubense is the pathogen that infects banana. a rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management ... | 2010 | 20471505 |
| the genomic organization of plant pathogenicity in fusarium species. | comparative genomics is a powerful tool to infer the molecular basis of fungal pathogenicity and its evolution by identifying differences in gene content and genomic organization between fungi with different hosts or modes of infection. through comparative analysis, pathogenicity-related chromosomes have been identified in fusarium oxysporum and fusarium solani that contain genes for host-specific virulence. lateral transfer of pathogenicity chromosomes, inferred from genomic data, now has been ... | 2010 | 20471307 |
| pathogenicity of fusarium isolates to striga hermonthica in burkina faso. | striga hermonthica (del.) benth. is an important constraint to cereal crop production in burkina faso, of which sorghum (sorghum bicolor l. moench) is the most important component. native fusarium species to use as bio-control agents to s. hermonthica has been investigated. fifty one fusarium isolates obtained from diseased plants of s. hermonthica were evaluated for their pathogenicity against striga under controlled environmental conditions. of 51 fusarium isolates, 14 were pathogenic to s. he ... | 2010 | 20464941 |
| the need for culture collections to support plant pathogen diagnostic networks. | plant-pathogenic microorganisms, by virtue of their size, similarity in disease symptoms and closely related morphologies, are notoriously difficult to diagnose and detect. diagnosis gives proof as to the causal agent of disease and is important for developing appropriate control measures. detection shows the presence of a microorganism and is of importance for safeguarding national and international trade. live reference collections are required to characterize the taxonomy and function of micr ... | 2010 | 20457251 |
| formation of fumonisins and other secondary metabolites by fusarium oxysporum and f. proliferatum: a comparative study. | the principal aim of this study was to estimate the formation of fumonisins (fb(1) and fb(2)), moniliformin (mon), and ergosterol (erg) by fusarium oxysporum and fusarium proliferatum, while the formation of beauvericin (bea) was estimated by the latter fusarium species only. moreover, the effect of temperature on the biosynthesis of mycotoxins was also evaluated. fumonisins were formed by f. proliferatum, with the highest yield at 18 degrees c (720.0-1976.6 microg g(-1) for fb(1), 74.2-670.8 mi ... | 2010 | 20455157 |
| antagonism of some aquatic hyphomycetes against plant pathogenic fungi. | the antagonistic activity of five aquatic hyphomycetes, viz., heliscus lugdunensis, tetrachaetum elegans, tetracladium breve, t. marchalianum, and t. nainitalense, against seven plant pathogenic fungi was studied using a dual culture technique. inhibitory activity of tested aquatic hyphomycetes was determined by measuring the radial growth of plant pathogenic fungi on dual culture plates. tetrachaetum elegans showed antagonistic activity against colletotrichum falcatum, fusarium oxysporum, pyric ... | 2010 | 20454756 |
| the two-component histidine kinase fhk1 controls stress adaptation and virulence of fusarium oxysporum. | fungal histidine kinases (hks) have been implicated in different processes, such as the osmostress response, hyphal development, sensitivity to fungicides and virulence. members of hk class iii are known to signal through the hog mitogen-activated protein kinase (mapk), but possible interactions with other mapks have not been explored. in this study, we have characterized fhk1, encoding a putative class iii hk from the soil-borne vascular wilt pathogen fusarium oxysporum. inactivation of fhk1 re ... | 2010 | 20447287 |
| the arms race between tomato and fusarium oxysporum. | the interaction between tomato and fusarium oxysporum f. sp. lycopersici has become a model system for the study of the molecular basis of disease resistance and susceptibility. gene-for-gene interactions in this system have provided the basis for the development of tomato cultivars resistant to fusarium wilt disease. over the last 6 years, new insights into the molecular basis of these gene-for-gene interactions have been obtained. highlights are the identification of three avirulence genes in ... | 2010 | 20447279 |
| antifungal effects of the bioactive compounds enniatins a, a(1), b, b(1). | to produce enniatin (ens), fusarium tricinctum cect 20150 was grown in a liquid medium of potato (pdb), being mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semipreparative column using a mobile phase of acetonitrile/water using gradient condition. the purity of the ens fractions was verified by analytical hplc and lc/ms-ms. the pure fractions of ens were utilized to study the biological activity on several mycotoxigenic moulds as fusarium verticilloides ... | 2010 | 20417654 |
| recombinant amaranth cystatin (ahcpi) inhibits the growth of phytopathogenic fungi. | phytocystatins are cysteine proteinase inhibitors from plants implicated in defense mechanisms against insects and plant pathogens. we have previously characterized an amaranth cystatin cdna and analyzed its response to different kinds of abiotic stress [37]. in order to characterize amaranth cystatin, the coding sequence was expressed in escherichia coli using the pqe-2 vector. recombinant cystatin was predominantly found in the soluble fraction of the cell extract. large amounts (266 mgl(-1)) ... | 2010 | 20403704 |
| when do arbuscular mycorrhizal fungi protect plant roots from pathogens? | arbuscular mycorrhizal (am) fungi are mainly thought to facilitate phosphorus uptake in plants, but they can also perform several other functions that are equally beneficial. our recent study sheds light on the factors determining one such function, enhanced plant protection from root pathogens. root infection by the fungal pathogen fusarium oxysporum was determined by both plant susceptibility and the ability of an am fungal partner to suppress the pathogen. the non-susceptible plant species (a ... | 2010 | 20400855 |
| co-composting of solid and liquid olive mill wastes: management aspects and the horticultural value of the resulting composts. | successful co-composting of solid and liquid olive mill wastes (omw) and obtaining a product of horticultural value may increase the viability of this recycling approach. two composting cycles were performed, in which olive mill solid wastes (omsw) were used to form five mixtures, wetted either with fresh water or with olive mill wastewater (omww). up to approximately 0.3m(3) of omww could be applied to each m(3) of the raw materials without negatively affecting the chemical, physical and hortic ... | 2010 | 20399644 |
| molecular characterization of fusarium oxysporum f. melongenae by issr and rapd markers on eggplant. | fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (solanum melongena). issr and rapd markers were used to characterize fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern turkey. those isolates were not pathogenic to tomato. pathogens were identified by their morphology, and their identity was confirmed by pcr amplification using the specific primer pf02-3. the isolates were classified into groups on the basis of issr and rapd fingerprin ... | 2010 | 20390339 |
| characterization of a novel plantain asr gene, mpasr, that is regulated in response to infection of fusarium oxysporum f. sp. cubense and abiotic stresses. | asr (abscisic acid, stress, ripening induced) genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid (aba) and injury; in addition to plant responses to developmental and environmental signals. we isolated an asr gene, mpasr, from a suppression subtractive hybridization (ssh) cdna library of cold induced plantain (musa paradisiaca) leaves. mpasr expression was upregulated in fusarium oxysporum f. sp. cubense infected plantain leaves, peels and ro ... | 2010 | 20377692 |
| the diversity and evolution of circadian clock proteins in fungi. | circadian rhythms are endogenous cellular patterns that associate multiple physiological and molecular functions with time. the neurospora circadian system contains at least three oscillators: the frq/wc-dependent circadian oscillator (fwo), whose core components are the frq, wc-1, wc-2, frh, and fwd-1 proteins; the wc-dependent circadian oscillator (wc-flo); and one or more frq/ wc-independent oscillators (flo). little is known about the distribution of homologs of the neurospora clock proteins ... | 2010 | 20361495 |
| fusarium oxysporum homogenates and jasmonate induce limited sanguinarine accumulation in argemone mexicana cell cultures. | in vitro cultures of argemone mexicana (papaveraceae) were induced from leaves of mature plants. sanguinarine, a benzophenanthridine, was the main alkaloid in the cultures, even in the absence of inducers of secondary metabolism. the accumulation of this metabolite was increased by adding methyl jasmonate and fungal elicitors, although in a limited fashion in comparison to other sanguinarine-producing species. evidence of a transport mechanism, which may be related to the magnitude of the respon ... | 2010 | 20349332 |
| investigation of factors influencing spore germination of paenibacillus polymyxa accc10252 and sqr-21. | bioorganic fertilizer containing paenibacillus polymyxa sqr-21 showed very good antagonistic activity against fusarium oxysporum. to optimize the role of p. polymyxa sqr-21 in bioorganic fertilizer, we conducted a study of spore germination under various conditions. in this study, l-asparagine, glucose, fructose and k(+) (agfk), and sugars (glucose, fructose, sucrose, and lactose) plus l-alanine were evaluated to determine their ability to induce spore germination of two strains; p. polymyxa acc ... | 2010 | 20309541 |
| development of impala-based transposon systems for gene tagging in filamentous fungi. | genome sequences of many filamentous fungi are now available and additional genomes are currently being sequenced. one of the next strategic goals is to generate collections of tagged genes in order to establish a link between the several thousands of predicted genes and their function. transposable elements have been invaluable for the identification and isolation of genes of interest as insertion of a transposon both disrupts and tags a gene. in an effort to exploit active transposons identifi ... | 2010 | 20238260 |
| ghwrky3, a novel cotton (gossypium hirsutum l.) wrky gene, is involved in diverse stress responses. | wrky proteins play important roles in plant defense responses. in the present study, a novel wrky gene, nominated as ghwrky3, was isolated from cotton (gossypium hirsutum l.). the full-length cdna of ghwrky3 is 1,705 bp in length and encodes a protein with 507 amino acids containing two typical wrky domains and two zinc finger motifs. amino acid sequence alignment revealed that ghwrky3 shares a high degree of identity with other higher plant wrky proteins. the subcellular localization assay indi ... | 2011 | 20238169 |
| comparative genomics reveals mobile pathogenicity chromosomes in fusarium. | fusarium species are among the most important phytopathogenic and toxigenic fungi. to understand the molecular underpinnings of pathogenicity in the genus fusarium, we compared the genomes of three phenotypically diverse species: fusarium graminearum, fusarium verticillioides and fusarium oxysporum f. sp. lycopersici. our analysis revealed lineage-specific (ls) genomic regions in f. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. ls regions are ... | 2010 | 20237561 |
| shifts in banana root exudate profiles after colonization with the non-pathogenic fusarium oxysporum strain fo162. | the non-pathogenic fungus fusorium oxysporum strain fo162 can efficiently colonize banana roots and reduce infecting by the burrowing nematode radopholus similis. it is assumed that the fungus triggers a systemic reaction in the plant, which is affecting the biochemical composition of the root exudates and is thus causing the reduction in nematode colonization. to characterize these shifts, a continuous flow experiment was set up to collect root metabolites on a matrix (xad-4). based on hplc ana ... | 2009 | 20222617 |
| plant tissue colonization by the fungus race 1.2 of fusarium oxysporum f.sp. melonis in resistant melon genotypes. | four melon accessions; 'shiroubi okayoma', 'c-211', 'k.n.m' and 'bg-5384', resistant to race 1.2 of fusarium oxysporum f.sp. melonis and a susceptible one 'piel de sapo' were tested to see which hypocotyl regions were invaded by the fungus, and to examine the relationship between resistance and presence of the pathogen in the plant tissue. while the fungus was shown to colonize all stem segments (either the upper, middle, or lower hypocotyl) of the susceptible and resistant plant accession, colo ... | 2009 | 20222554 |
| susceptibility of chrysanthemum and paris daisy varieties to several isolates of fusarium oxysporum f. sp. chrysanthemi. | fusarium oxysporum f.sp. chrysonthemi is a pathogen recently reported in italy on four economically important ornamental crops belonging to the compositae family: chrysanthemum (chrysanthemum morifolium), paris daisy (argyranthemum frutescens), african daisy (osteospermum sp.) and gerbera (gerbera jamesonii). the risk of transmission of the pathogen among these species is high because the hosts are frequently cultivated in the same nursery. the susceptibility of 24 paris daisy and 12 chrysanthem ... | 2009 | 20222547 |
| effect of plant age on endophytic bacterial diversity of balloon flower (platycodon grandiflorum) root and their antimicrobial activities. | balloon flower (platycodon grandiflorum) is widely cultivated vegetable and used as a remedy for asthma in east asia. experiments were conducted to isolate endophytic bacteria from 1-, 3-, and 6-year-old balloon flower roots and to analyze the enzymatic, antifungal, and anti-human pathogenic activities of the potential endophytic biocontrol agents obtained. total 120 bacterial colonies were isolated from the interior of all balloon flower roots samples. phylogenetic analysis based on 16s rrna ge ... | 2010 | 20221603 |
| antifungal activities of ethanolic extract from jatropha curcas seed cake. | phorbol ester extraction was carried out from jatropha curcas seed cake, a by-product from the bio-diesel fuel industry. four repeated extractions from 5 g j. curcas seed cake using 15 ml of 90% (v/v) ethanol and a shaking speed of 150 rev/min gave the highest yield of phosbol esters. the ethanolic extract of j. curcas seed cake showed antifungal activities against important phytofungal pathogens: fusarium oxysporum, pythium aphanidermatum, lasiodiplodia theobromae, curvularia lunata, fusarium s ... | 2010 | 20208435 |
| a rapid method for isolation of total dna from pathogenic filamentous plant fungi. | dna isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. we have developed a fast dna isolation protocol for fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for pyrenochaeta terrestris, which causes pink root in onions. this protocol was based on the sodium dodecyl sulfate/phenol method, without beta-mercaptoethanol and without maceration in liquid ... | 2010 | 20198572 |
| purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom clitocybe sinopica. | a novel antibacterial protein with a molecular mass of 44 kda has been isolated from dried fruiting bodies of the wild mushroom clitocybe sinopica. sodium dodecyl sulfate/polyacrylamide gel electrophoresis showed that the protein was composed of two subunits each with a molecular mass of 22 kda. its n-terminal amino-acid sequence, svqatvngdkml, has not been reported for other antimicrobial proteins. the purification protocol included ion exchange chromatography on deae-cellulose, cm-cellulose an ... | 2010 | 20198215 |
| use of the plant defense protein osmotin to identify fusarium oxysporum genes that control cell wall properties. | fusarium oxysporum is the causative agent of fungal wilt disease in a variety of crops. the capacity of a fungal pathogen such as f. oxysporum f. sp. nicotianae to establish infection on its tobacco (nicotiana tabacum) host depends in part on its capacity to evade the toxicity of tobacco defense proteins, such as osmotin. fusarium genes that control resistance to osmotin would therefore reflect coevolutionary pressures and include genes that control mutual recognition, avoidance, and detoxificat ... | 2010 | 20190074 |
| a case of primary localized cutaneous infection due to fusarium oxysporum. | fusarium is a ubiquitous hyalohyphomycete isolated from food, widespread in the environment (plants, soil) and present at all latitudes. fusarium oxysporum and fusarium solani are the most frequent pathogenic species, followed by f. moniliforme and f. chlamydosporum. infections due to this mold may be disseminated or localized. localized forms include cutaneous and subcutaneous infection, onychomycosis, endophtalmitis, otitis, sinusitis, arthritis, osteomyelitis, and brain abscess. disseminated ... | 2010 | 20177971 |
| bioaccumulation and biovolatilisation of pentavalent arsenic by penicillin janthinellum, fusarium oxysporum and trichoderma asperellum under laboratory conditions. | some fungi are able to control and remediate arsenic (as)-contaminated soil, sediment, or water. here, we investigate potential accumulation and volatilisation of as by three fungi strains. results indicated that the highest level of as was accumulated by penicillin janthinellum with 39.54 μg after 10 days in the culture system amended with 2,500 μg as(v), which represents 50 mg/l as. fusarium oxysporum showed the highest amount of volatilised as with 304.06 μg after 15 days. the as content in t ... | 2010 | 20155358 |
| the influence of environmental factors on growth and interactions between embellisia allii and fusarium oxysporum f. sp. cepae isolated from garlic. | embellisia allii results in the formation of a bulb canker and black soot on the surface of different alliums and it has been frequently detected on garlic bulbs together with the spoilage fungus, fusarium oxysporum f. sp. cepae, which causes bulb basal plate rot. in this study, the influence of water activity (a(w)) and temperature on mycelial growth of e. allii and f. oxysporum f. sp. cepae, conidial size and sporulation of e. allii, interactions between e. allii and f. oxysporum f. sp. cepae, ... | 2010 | 20153538 |
| synthesis and fungicidal activity of aryl carbamic acid-5-aryl-2-furanmethyl ester. | chitin, a major structural component of insect cuticle and fungus cell wall but absent in plants and vertebrates, is regarded as a safe and selective target for pest control agents. chitin synthesis inhibitors (csis) have been well-known as insect growth regulators (igrs) but rarely found as fungicides in agriculture. to find novel csis with good activity, benzoylphenylurea, a typical kind of csis, was chosen as the lead compound and 26 novel aryl carbamic acid-5-aryl-2-furanmethyl esters were d ... | 2010 | 20151651 |
| resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing arabidopsis npr1. | cotton is an economically important crop worldwide that suffers severe losses due to a wide range of fungal/bacterial pathogens and nematodes. given its susceptibility to various pathogens, it is important to obtain a broad-spectrum resistance in cotton. resistance to several fungal and bacterial diseases has been obtained by overexpressing the non-expressor of pathogenesis-related genes-1 (npr1) in various plant species with apparently minimal or no pleiotropic effects. we examined the efficacy ... | 2010 | 20151323 |
| phenotypic analysis of arabidopsis mutants: oomycete pathogens. | various fungal pathogens are used in arabidopsis pathogen studies, including fusarium oxysporum, alternaria brassicicola, botrytis cinerea, and others. the oomycete pathogen peronospora parasitica has been used by several groups and is described in this protocol. working with peronospora is complicated by the fact that it is an obligate biotroph, and consequently cultures must be maintained on living plants. there is no central repository for peronospora stocks, but most investigators who work w ... | 2009 | 20147042 |
| primary metabolism of chickpea is the initial target of wound inducing early sensed fusarium oxysporum f. sp. ciceri race i. | biotrophic interaction between host and pathogen induces generation of reactive oxygen species that leads to programmed cell death of the host tissue specifically encompassing the site of infection conferring resistance to the host. however, in the present study, biotrophic relationship between fusarium oxysporum and chickpea provided some novel insights into the classical concepts of defense signaling and disease perception where ros (reactive oxygen species) generation followed by hypersensiti ... | 2010 | 20140256 |
| synthesis of nalidixic acid based hydrazones as novel pesticides. | thirty-one substituted hydrazones of nalidixic acid hydrazide were synthesized and characterized by spectral techniques. these compounds were evaluated for various biological activities, namely, fungicidal, insecticidal, and nitrification inhibitory activities. the antifungal activity was evaluated against five pathogenic fungi, namely, rhizoctonia bataticola , sclerotium rolfsii , rhizoctonia solani , fusarium oxysporum , and alternaria porii . they showed maximum inihibition against a. porii w ... | 2010 | 20131903 |
| hypersensitive response of sesamum prostratum retz. elicitated by fusarium oxysporum f. sesame (schelt) jacz butler. | aim of this study was to investigate the intensity and timing of the ros formation, lipid peroxidation and expression of antioxidant enzymes as initial responses of calli of sesamum prostratum (sp) against fusarium oxysporum f. sesame crude toxin metabolite of varying concentrations. 2,4 dichlorophenoxy acetic acid (2,4-d) / coconut milk combinations were found to be more efficient among different hormonal regimes (2,4 -d, 2,4-d/casein hydrosylate and 2,4-d/ coconut milk). the concentration of h ... | 2009 | 20112812 |
| the transposon impala is activated by low temperatures: use of a controlled transposition system to identify genes critical for viability of aspergillus fumigatus. | genes that are essential for viability represent potential targets for the development of anti-infective agents. however, relatively few have been determined in the filamentous fungal pathogen aspergillus fumigatus. a novel solution employing parasexual genetics coupled with transposon mutagenesis using the fusarium oxysporum transposon impala had previously enabled the identification of 20 essential genes from a. fumigatus; however, further use of this system required a better understanding of ... | 2010 | 20097738 |
| a thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against verticillium dahliae and other stresses in transgenic tobacco. | for the first time, a sea-island cotton (gossypium barbadense l.) thaumatin-like protein gene (gbtlp1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. the presence of the transgene was verified by southern blotting and higher expression levels of gbtlp1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. transgenic plants with constitutively higher ... | 2010 | 20097164 |
| [dihydroxylation of dehydroepiandrosterone in positions 7alpha and 15alpha by mycelial fungi]. | the ability of 485 fungal strains is studied for catalysis of the process of 7alpha, 15alpha-dihydroxylation of dehydroepiandrosterone (dhea, 3alpha-hydroxy-5-androstene-17-one), a key intermediate of the synthesis of physiologically active compounds. the ability for the formation of 3alpha, 7alpha, 15alpha-trihydoxy-5-androstene-17-one (7alpha, 15alpha-di-oh-dhea) was found for the first time for representatives of 12 genera, eight families, and six orders of ascomycetes, eight genera, four fam ... | 2009 | 20067153 |
| stylicins, a new family of antimicrobial peptides from the pacific blue shrimp litopenaeus stylirostris. | the present study reports the characterization of ls-stylicin1, a novel antimicrobial peptide from the penaeid shrimp, litopenaeus stylirostris. the predicted mature peptide of 82 residues is negatively charged (theoretical pi=5.0) and characterized by a proline-rich n-terminal region and a c-terminal region containing 13 cysteine residues. the recombinant ls-stylicin1 has been isolated in both monomeric and dimeric forms. both display strong antifungal activity against fusarium oxysporum (1.25 ... | 2010 | 20061030 |
| methyl salicylate production in tomato affects biotic interactions. | the role of methyl salicylate (mesa) production was studied in indirect and direct defence responses of tomato (solanum lycopersicum) to the spider mite tetranychus urticae and the root-invading fungus fusarium oxysporum f. sp. lycopersici, respectively. to this end, we silenced the tomato gene encoding salicylic acid methyl transferase (samt). silencing of samt led to a major reduction in samt expression and mesa emission upon herbivory by spider mites, without affecting the induced emission of ... | 2010 | 20059742 |