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chorionic mycoplasmas and prematurity.in this study, recovery of ureaplasma urealyticum and mycoplasma hominis from the freshly exposed chorion following delivery was related to labor length but not to the interval between membrane rupture and delivery, to birth weight or to gestational age.19863795198
effect of carbohydrates on growth of ureaplasma urealyticum and mycoplasma hominis.we examined the effect of 31 carbohydrates on the growth of ureaplasma urealyticum and mycoplasma hominis. arbutin and its breakdown product, hydroquinone, inhibited growth of both species; the other substrates did not alter the extent of growth. volatile and nonvolatile end products of carbohydrate metabolism were not detected by gas chromatography.19873793871
role of genital mycoplasmas in young infants with suspected sepsis.to establish the prevalence of mycoplasma hominis and ureaplasma urealyticum in infants up to 3 months of age with suspected sepsis, blood, cerebrospinal fluid, and urine specimens from 203 patients with clinical signs and symptoms of sepsis were cultured for mycoplasma in addition to routine bacterial cultures. proved bacterial infections were identified in 24 patients, four of whom had bacteremia. m. hominis and u. urealyticum were not isolated from any of the 191 blood and 199 csf specimens t ...19863783341
[the role of intrauterine contraceptive devices in the development of inflammatory processes in the small pelvis].the incidence of pelvic inflammatory disease (pid) attributable to iud use has been increasing, especially after the removal of the dalkon shield from the market, but this relationship has not been settled conclusively. in recent decades pid included a variety of infections, but lately the definition of pid has meant acute ascending infections of the female genital tract. its most common risk factors include promiscuity of iud use, although this can be reduced to one fourth by regular checkups ...19863763205
antimicrobial susceptibility testing of mycoplasma hominis. 19863759152
treatment of sexually transmitted diseases.this paper outline the treatments of choice for sexually transmitted infections. current treatment recommendations for gonorrhea reflect the emergence of tetracycline-resistant neisseria gonorrhoeae and the increasing prevalence of penicillinase-producing strains that are resistant to penicillin. a single dose of amoxicillin is highly effectice against urethral or cervical gonorrhea caused by penicillin-susceptible strains; also effective is a new agent, ceftriaxone. however, neither of these ...19863754031
clinical and microbiological investigation of women with acute salpingitis and their consorts.to determine the microbiological causes of pelvic inflammatory disease, 43 women with acute salpingitis (as) and 160 controls were studied. amongst as women there were significantly higher endocervical isolation rates of neisseria gonorrhoeae (54% vs 6%), chlamydia trachomatis (40% vs 13%), mycoplasma hominis (60% vs 19%), enterobacteria (26% vs 11%) and anaerobic bacteria (58% vs 29%). a polymicrobial pathogenic endocervical flora was present in both gonococcal and non-gonococcal as. laparoscop ...19863741814
genital mycoplasma colonization in neonatal girls.specimens obtained from the genital tract of 90 pairs of mothers and their female infants were cultured for ureaplasma urealyticum (ureaplasma) and mycoplasma hominis (m.hominis). ureaplasma and m.hominis were isolated from 80% and 17% of pregnant women, and 41% and 4.4% of female infants, respectively. fifty nine per cent of infants were positive for ureaplasma when they were borne per vaginam by ureaplasma-positive mothers. however, ureaplasma was not isolated from any of 11 infants delivered ...19863739634
hormonal status and mycoplasma colonization in the female genital tract.three hundred seventy-four cervical or vaginal specimens from patients with various hormonal states were cultured for ureaplasma urealyticum and mycoplasma hominis. significantly, low recovery rates of u urealyticum were obtained in the prepuberty (5%), puerperium (24%), and postmenopause (25%) groups, whereas pregnant women showed the highest incidence of u urealyticum (82%). the recovery rate of u urealyticum from neonates was 42%. the incidence of u urealyticum in sexually inactive females wa ...19863737042
prevalence of chlamydia trachomatis and other micro-organisms in women seeking abortions in pittsburgh, pennsylvania, united states of america.the prevalence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, group b streptococcus, herpes simplex virus, and neisseria gonorrhoeae from cervical cultures obtained from 210 women seeking abortion in pittsburgh, pennsylvania, united states of america was 9.3%, 72.9%, 25.2%, 4.3%, 0.9%, and 0.9% respectively. cultures from 40/203 (19.7%) patients failed to produce any of these organisms. c trachomatis isolation was not associated with age, race, marital status, average fami ...19863721515
[incidence of ureaplasma urealyticum and mycoplasma hominis in persons examined for sterility]. 19863719732
vaginal colonization with mycoplasma hominis and ureaplasma urealyticum.vaginal cultures obtained from unselected young women who consulted the gynecologist in a student health service were examined for ureaplasma urealyticum and mycoplasma hominis. each participant completed a confidential questionnaire. multiple logistic regression analysis was used to determine which variables, of a large number ascertained, were associated with mycoplasmal colonization. u. urealyticum was isolated from 273 (56.8%) of 481 participants. the following variables were significantly p ...19863715677
[incidence of mycoplasma hominis in women using an iud]. 19863714257
colonization with genital mycoplasmas in pregnant women and their neonates and birth weight.the colonization by genital mycoplasmas of mothers and their newborn infants was examined in 114 pregnant women and their 84 neonates. urine and cervical swabs were taken from the pregnant women in the last trimester, and urine from the neonates within six days after birth. ureaplasma urealyticum was found in 73.7% of the pregnant women and in 17.9% of the neonates. mycoplasma hominis was isolated in 8.8% of the material from the pregnant women and in 1.2% of that from the neonates. the isolatio ...19873673336
[biologic properties of ureaplasma urealyticum and mycoplasma hominis isolated from persons with inflammatory conditions of the urethra. ii. occurrence of ureaplasma urealyticum and mycoplasma hominis in healthy men and in those with inflammatory conditions of the urethra and in their sexual partners]. 19873669804
[biologic properties of ureaplasma urealyticum and mycoplasma hominis isolated from persons with inflammatory conditions of the urethra. i. a method of isolating ureaplasma and mycoplasma from human clinical specimens]. 19873669803
the role of mycoplasmas, ureaplasmas and chlamydiae in the genital tract of women presenting in spontaneous early preterm labour.the genital carriage of ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis was assessed in 72 women admitted to hospital in spontaneous preterm labour and in 26 women requiring preterm delivery for other reasons who formed a control group. women in preterm labour significantly more often carried ureaplasmas, had large numbers of m. hominis and subsequently developed chorioamnionitis than women in the control group. m. hominis, in particular, occurred more frequently and in larg ...19873669058
mycoplasma hominis meningitis in a preterm infant. 19873668264
an improved method for elimination of mycoplasmas from cell cultures.cell lines infected by different species of mycoplasma (mycoplasma orale, mycoplasma hominis) were decontaminated by co-culture with human blood monocyte (bm)-derived macrophages and pooled human immunoglobulin preparations. co-cultures with bm-derived macrophages or murine peritoneal macrophages (pm) alone were not successful. the phenotype of infected cell lines did not differ from that of uninfected cell lines as revealed by morphological, enzymecytochemical, and immunocytochemical analysis.19873668257
western blot analysis of the human serum response to mycoplasma hominis.mycoplasma hominis is a human genital pathogen with importance in postpartum pregnancy complications (postpartum fever/endometritis). previous research has suggested that serum antibody levels to m. hominis are important in predicting which groups of women are at risk. m. hominis strain pg21 was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and western blots to determine which protein antigens would be good candidates for use in serologic tests. western blots o ...19873667228
analysis of protein antigens of mycoplasma hominis: detection of polypeptides involved in the human immune response.immunoblotting of polypeptide antigens after polyacrylamide gel electrophoresis was used to analyze clinical strains of mycoplasma hominis. there was increased antibody titer for polypeptides comprising a wide range of molecular sizes between paired sera obtained from patients with indication of invasive infection. eight of these antigens were shown to be candidate surface antigens. absorption of hyperimmune rabbit serum with intact cells resulted in elimination or reduction in intensity of band ...19873667227
postcesarean wound infection by mycoplasma hominis in a patient with persistent postpartum fever.mycoplasma hominis was isolated in pure culture from a wound infection following delivery by cesarean section. the importance of recognizing this organism as a potential pathogen of the female genital tract is emphasized. two commercially available isolation systems that allow the recovery of this organism are also described.19873652655
[isolation of mycoplasma hominis from the blood in puerperal fever]. 19873614885
a microbiologic and clinical study of placental inflammation at term.this study describes the relationships between histologically evident inflammation of the placenta, membranes, and umbilical cords, and correlates these lesions with clinical outcome and with amniotic fluid and amniotic membrane cultures in pregnancies at risk for clinical infection. the overall frequency of inflammation in 123 placentas was 85.45%. there were good interrelationships between inflammatory lesions at various sites. membrane infiltrates of 3+ were seen in 90.9% of intra-amniotic in ...19873601279
prevalence and significance of mycoplasma hominis and ureaplasma urealyticum in the urines of a non-venereal disease population.ureaplasma urealyticum organisms (ureaplasmas) and mycoplasma hominis organisms (mycoplasmas) were sought in mid-stream urines collected from 200 men and 200 women attending hospital with conditions of a non-venereal nature. in addition, the urines from 100 male and 100 female healthy volunteers were examined. overall, ureaplasmas were isolated four times more often than mycoplasmas. in individuals less than 50 years of age, the organisms were found in about 20% of men and about 40% of women. in ...19873595751
[mycoplasmas and pregnancy].the pathogenic role of mycoplasms during pregnancy remains quite controverted, depending on the studies; for some it has an incidence on prematurity, delayed growth in utero and premature rupture of the membranes. the purpose of this study was, from a population of patients with term delivery, without any specific pathology, to verify the frequency of mothers carrying mycoplasma hominis or ureaplasma, and to determine the possible consequences on the newborn. a linear analysis of the evolution o ...19873589355
[incidence of mycoplasma hominis and ureaplasma urealyticum in pregnant women and gynecologic patients and their treatment with doxycycline]. 19873587970
bacteraemia and pelvic infection in women due to ureaplasma urealyticum and mycoplasma hominis.during a 22-month period, pelvic infection with bacteraemia that was due to genital mycoplasmas was diagnosed in 12 adult patients at the royal women's hospital. mycoplasma hominis was isolated from seven patients and ureaplasma urealyticum from five patients. infections occurred postpartum in seven patients (in three patients after a vaginal delivery and in four patients after a caesarean section) and after gynaecological procedures in five patients. all patients were moderately ill. during the ...19873574192
infection with multiple sexually transmitted agents.a patient named as a contact by an individual with gonorrhea and syphilis was found to be an asymptomatic carrier of seven organisms known to be transmitted sexually. cultures were positive for neisseria gonorrhoeae, chlamydia trachomatis, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and herpes simplex virus. trichomonas vaginalis was detected by a wet preparation and the papanicolaou smear. the cervix was found to have an ulcer on the posterior lip that yielded herpes simp ...19873573062
colonization of sexually abused children with genital mycoplasmas.although sexually abused children are usually evaluated only for the presence of infections with neisseria gonorrhoeae and treponema pallidum, they are also at risk for acquiring other sexually transmitted organisms prevalent in the adult population. accordingly, we examined pharyngeal, anorectal and genital specimens from 50 children who had been sexually abused and from 40 healthy children who served as controls; these specimens were cultured for mycoplasma hominis and ureaplasma urealyticum. ...19873563830
mycoplasmas and in vitro fertilization.semen samples taken from 135 patients attending an in vitro fertilization clinic were shown to be colonized, 53 with ureaplasma urealyticum (39%) and 16 with mycoplasma hominis (12%). an unidentified mycoplasma species was isolated from the sperm of two patients. m. hominis was recovered from all the washed sperm samples taken from colonized semen, whereas washing the sperm eradicated u. urealyticum from 71% of colonized semen. the presence of mycoplasmas in semen made no significant difference ...19873552747
[in vitro activity of nitroxoline on urogenital mycoplasmas].the product nitroxoline was studied in vitro for its activity towards ureaplasma urealyticum and mycoplasma hominis. in view of the low mic values obtained, it seems nitroxoline could be used in the treatment of urinary infections. it is bactericidal, and should not produce resistant strains.19863543811
effect on birth weight of erythromycin treatment of pregnant women.to test the hypothesis that treatment with antibiotics prevents low birth weight, pregnant women whose vaginal cultures contained ureaplasma urealyticum or mycoplasma hominis (or both) and who gave written informed consent were treated with one of the following: identical looking capsules containing 250 mg of either erythromycin estolate or stearate (active against u urealyticum), or 150 mg of clindamycin hydrochloride (active against m hominis), or placebo, four times daily for six weeks in a r ...19873543767
sternotomy infections with mycoplasma hominis.mycoplasma hominis caused sternal wound infections with mediastinitis in three patients. one infection occurred in a nonimmunocompromised man after coronary artery bypass grafting. the wound did not heal after repeated debridement, closed irrigation of the mediastinum with povidone-iodine solution, and antimycoplasmal chemotherapy; muscle flap grafting was eventually required. two infections occurred in recipients of heart-lung transplants after the isolation of mycoplasma from bronchial secreti ...19873541722
ureaplasma urealyticum as a cause of postpartum fever.mycoplasma hominis has been shown to cause a postpartum fever in nonimmune women and among the approximately 5% of febrile postpartum women who have the organism isolated from the blood. ureaplasma urealyticum causes postpartum fever in the 20% of febrile women with u. urealyticum alone isolated from the endometrium. about 5% of febrile postpartum women have u. urealyticum isolated from the blood. these data suggest that both genital mycoplasmas can cause postpartum fever.19863540898
[chlamydia trachomatis in patients with genital contact infections in austria--studies of 3,367 patients].in order to evaluate the epidemiological importance of chlamydia trachomatis (c. trachomatis) as a genital microorganism, data were obtained from 3,367 patients with sexually transmitted diseases in vienna and analyzed by computer-assisted methods. c. trachomatis was cultured in 26.1% of 2,594 patients investigated for the first time. the microorganism was found more often in male patients (31.3%) than in female patients (21%). 32.2% of positive chlamydia cultures were obtained from patients wit ...19863538672
aetiology of urinary symptoms in sexually active women.two hundred and fifty six unselected women, 50 of whom had urinary symptoms (frequency of urination or dysuria, or both), and who were attending a department of genitourinary medicine, were investigated. the urinary symptoms were associated both with pyuria and the isolation of undoubted pathogens from midstream urine (msu) specimens. no associations were found between urinary symptoms and the isolation of neisseria gonorrhoeae or chlamydia trachomatis from the urethra or cervix; the recovery of ...19863533756
[amine colpitis].significance of koh test and isonitril (isocyanide) reaction tested in 104 patients was compared with our microbial culture results. in gardnerella vaginalis as well as in no spores forming anaerobic germs the koh test was not and the isonitril reaction was only insignificant reliable. both tests are not able to substitute the procedures of culturing germs including those of mycoplasma hominis and ureaplasma urealyticum.19863532626
further studies on genital mycoplasmas in intra-amniotic infection: blood cultures and serologic response.mycoplasma hominis is recovered significantly more often in amniotic fluid of women with intra-amniotic infection than in matched control women, but ureaplasma urealyticum is found in 50% of amniotic fluid samples of both groups. to gain further understanding, we performed blood cultures for genital mycoplasmas and measured serologic responses by a micro enzyme-linked immunosorbent assay method in women with intra-amniotic infection and in control subjects. in blood cultures of 81 women with int ...19863515945
effect of short term treatment of non-gonococcal urethritis with minocycline.two hundred and forty four men with non-gonococcal urethritis (ngu) took part in a trial of minocycline. chlamydiae were isolated from 34%, ureaplasmas from 47.1%, and mycoplasma hominis from 10.2%. these micro-organisms were isolated least often from men suffering their third or more attack of ngu, and men in this group failed most often to respond to minocycline. irrespective of the micro-organisms isolated originally or the number of previous attacks, the failure rate (10%) for men receiving ...19863512418
a dna probe for detecting mycoplasma genitalium in clinical specimens.despite decades of careful study, the etiologies of all cases of pelvic inflammatory disease (pid) and non-gonococcal urethritis (ngu) have yet to be described. mycoplasma genitalium is a newly described organism which has been implicated as a cause of both pid and ngu. because of fastidious growth requirements, prolonged incubation time and frequent overgrowth in clinical specimens by mycoplasma hominis, non-culture methods need to be developed for its detection. we have cloned m. genitalium dn ...19873453423
urinary tract infection (uti) in a couple caused by mycoplasma hominis and ureaplasma urealyticum (a case report). 19873449457
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethral inflammations. iv. occurrence of serological variants of m. hominis among the strains isolated from healthy men those with urethral inflammations and their sexual partners]. 19873437786
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethral inflammations. iii. occurrence of serological types of u. urealyticum among the strains isolated from healthy men those with urethral inflammations, and their sexual partners]. 19873437785
isolation of genital mycoplasmas from the blood of neonates and women with pelvic infection using conventional sps-free blood culture media.standard blood culture media used in our laboratory were tested for their ability to support the growth of mycoplasma hominis and ureaplasma urealyticum. small inocula (approximately 10 colony forming units per ml) of both organisms grew in diphasic tryptone soya medium but not in any of several media containing sodium polyanetholesulphonate (sps) including a modified schaedler broth (rwh anaerobic medium) and two bactec media (6b and 7d). both organisms were inhibited even by very low concentra ...19873431914
[new diagnostic and therapeutic directions in so-called essential anorectal pain].the authors report a study of 40 patients affected with "essential" ano-rectal neuralgia. it manifests itself by frequent urinary disorders discovered most of the time at the interview of the patient and emphasizes in many cases the presence of psychosomatic disorders. this study has also demonstrated frequent abnormalities of laboratory tests including: chlamydiae serology, urethral samplings and cyto-bacteriological examination of urine looking for: chlamydiae, ureaplasma urealyticum, mycoplas ...19873426144
urinary tract symptoms: microbiologic evaluation in rural family practice.in order to define the etiology of urinary symptoms in rural family practice, this study examines 106 patients (88 women, 18 men) who went to their family physicians in private practice or a resident-faculty practice with genitourinary symptoms. evaluation of each patient included history, physical examination, urinalysis, and urine or cervical cultures for bacteria, mycoplasma, and chlamydia. using agar plate culturing techniques, 37 patients (35 percent) were identified as having significant u ...19883414387
[mycoplasma infections of the kidneys].from the urine cultures of 1238 patients with renal or suspicious for renal disease mycoplasmas were isolated in 119 (9.6%). in 75 patients the mycoplasmas were typified. in 56 patients m. hominis and in 24 patients ur. urealyticum were isolated. in 52 patients there were data for pyelonephritis, in 46 patients there was no renal infection. in 21 patents there were mycoplasmas and arterial hypertension without sufficient data for pyelonephritis. in the group of patients with pyelonephritis and a ...19883414078
hematoma infected with mycoplasma hominis.although mycoplasmas are commonly isolated in the genitourinary and upper respiratory tracts, they are rarely isolated from other sites. we describe a case of a young woman with trauma to her pelvis and genitourinary tract who had a thigh hematoma infected with mycoplasma hominis. the patient had persistent systemic symptoms that resolved with appropriate antibiotics and surgical drainage. this case as well as other cases of mycoplasma-infected hematomas point out the need to consider mycoplasma ...19883399998
distinction of species and strains of mycoplasmas (mollicutes) by genomic dna fingerprints with an rrna gene probe.genomic fingerprints of acholeplasma laidlawii, mycoplasma hominis, and mycoplasma pneumoniae strains were obtained by southern blot hybridization of the digested mycoplasmal dnas with an rrna gene probe. the hybridization patterns revealed genotypic heterogeneity among a. laidlawii and m. hominis strains and a remarkable degree of homogeneity among m. pneumoniae strains isolated from pneumonia patients during a 10-year period. genomic fingerprints with the rrna gene probe can thus serve as indi ...19883384931
isolation of genital mycoplasmas and chlamydia trachomatis in stillborn and neonatal autopsy material.chlamydia trachomatis and the genital mycoplasmas are significantly prevalent in sexually active women. how these organisms may affect the outcome of pregnancy and the neonate was the principal thrust of this investigation. placenta, liver, and lung tissue were cultured from mycoplasma hominis, ureaplasma urealyticum, chlamydia trachomatis, and aerobic as well as anaerobic bacteria in 432 stillborn and neonatal autopsies. genital mycoplasmas were isolated from 36 cases (8.3%). acute chorioamnion ...19883382330
evidence that chlamydia trachomatis causes seronegative arthritis in women.chlamydia trachomatis elementary bodies (ebs) were found in synovial membranes or synovial fluid cell deposits from five of 15 women with seronegative mono- or oligoarthritis by means of a fluorescein conjugated anti-chlamydial monoclonal antibody (micro trak; syva). genital tract specimens were taken from only five of the patients, one of whom had intra-articular ebs, but none was chlamydia positive. six of 10 patients tested were hla-b27 positive, and chlamydial igg antibody, measured by micro ...19883365028
mycoplasma hominis septicemia after heart surgery. 19883364454
association of coexisting vaginal infections and multiple abusers in female children with genital warts.all 11 girls less than 12 years of age presenting to the pediatric clinic between 1980 and 1986 with genital warts were evaluated by the child protection team for possible sexual abuse. nine of the 11 girls had condylomata acuminata, and two had verruca vulgaris. ten of the 11 had historical and/or physical evidence other than the warts that confirmed sexual abuse. six girls had coexisting vaginal infections such as bacterial vaginosis and infections due to various pathogens including neisseria ...19883358240
mycoplasma hominis surgical wound infection: a case report and discussion.a patient with mycoplasma hominis infection of a clean surgical wound that contained a prosthetic vascular graft had clinical findings similar to those observed in patients with acute bacterial infections. etiologic diagnosis was delayed because m. hominis is not appreciated as a cause of surgical wound infections and because of its special growth characteristics. confirmation of m. hominis infection requires awareness on the part of the clinician and assistance from an informed microbiologist. ...19883340994
extragenital mycoplasma hominis infection: a report of two cases.mycoplasma hominis was isolated from the surgical wounds of two patients. one of these patients developed a septicaemia-like illness after coronary artery vein-graft surgery. mycopl. hominis was isolated from the sternotomy wound and pleural fluid of this patient but not from his blood. no other pathogens were isolated. the second patient developed a low-grade soft-tissue infection due to mycopl. hominis in a craniotomy wound; his condition responded to tetracycline treatment. the source of infe ...19883340029
antimicrobial activity of amniotic fluid against chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum.amniotic fluids obtained by amniocentesis at 16 weeks to term were examined for the presence of chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum. of 140 fluids tested, none harbored chlamydiae, and only one harbored mycoplasma, m. hominis. a number of amniotic fluids were subsequently tested for their ability to inhibit the growth of these microorganisms. amniotic fluids and chlamydial suspensions in a 2:1 ratio were incubated 30 to 90 minutes before their inoculation in mcc ...19883337164
a review of the in-vitro activity of roxithromycin against genital pathogens.the in-vitro activity of roxithromycin against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, treponema pallidum, gardnerella vaginalis and haemophilus ducreyi is reviewed. roxithromycin demonstrated equivalent activity to erythromycin against n. gonorrhoeae, c. trachomatis, m. hominis, u. urealyticum, g. vaginalis and h. ducreyi. in a rabbit model for syphilis, potentially useful activity against t. pallidum has been demonstrated.19873323169
roxithromycin in nongonococcal urethritis.this presentation is a summary of five different studies on the efficacy and tolerance of roxithromycin in the treatment of non-gonococcal genital infections. three of the studies were double-blind comparative and two were open studies. of the 924 out-patients whose data were analysed for clinical efficacy, 637 received treatment with roxithromycin 150 mg bd. the standard dose of roxithromycin, 150 mg bd for ten days, was compared with doxycycline 200 mg daily, lymecycline 300 mg bd and roxithro ...19873323168
[genital mycoplasma infections--clinical aspects, diagnosis and therapy].ureaplasma urealyticum und mycoplasma hominis are frequently isolated from the urogenital tract of sexually active persons. their pathogenetic role in urogenital infections and infertility are still controversial. u. urealyticum can cause urethritis and most likely also chronic prostatitis. m. hominis can cause cystitis, salpingitis, and postpartal fever in women and meningitis in newborns. m. genitalium was recently isolated from patients with urethritis and salpingitis. the diagnosis is made b ...19873318082
[detection and identification of mycoplasma infections by dna hybridization].infection of cell cultures by mycoplasmas can be detected by hybridization of the dna of suspected cell cultures with recombinant plasmids containing fragments of the mycoplasma dna. the test is very sensitive and allows detection of as little as 1 ng of mycoplasmal dna, roughly equivalent to the dna amount of 10(6) mycoplasmas. this approach turns out to be effective for detection and identification of mycoplasmas in clinical material, plant and insect tissues. a set of dna probes for detection ...19873318046
mycoplasma hominis in patients with pelvic inflammatory disease.the role of mycoplasma hominis in pelvic inflammatory disease (pid) was studied among 35 patients with laparoscopically confirmed salpingitis or histopathologically confirmed plasma cell endometritis. m. hominis was isolated from the cervix of 15 patients, from the rectum of 13 patients, and from the endometrium of 5 patients. only two of these five patients had m. hominis isolated from the endometrium in the absence of chlamydia trachomatis or neisseria gonorrheae, the major pid-causing organis ...19873312109
dna probes for the detection of mycoplasmas in genital specimens.the utility of whole-genomic dna probes for the detection of infections by genital mycoplasmas was investigated in 220 men attending a sexually transmitted diseases clinic. in 144 patients, probe results were compared with quantitative culture results. the prevalence of mycoplasma hominis was 11% by culture, whereas the prevalence of ureaplasmas was 38%. the m. hominis dna probe detected 9 of 16 m. hominis culture-positive specimens and 2 of 128 culture-negative specimens. the ureaplasma urealyt ...19873312098
attachment of mycoplasma hominis to human cell cultures.clinical isolates, cell-culture contaminants, and the type strain pg21 of mycoplasma hominis were examined for attachment to erythrocytes and human cell cultures. strain 13428 (from blood, postpartum fever) and strain 1184 (cell culture) attached to human and guinea pig erythrocytes, but there were no differences in attachment activities between these strains. however, five m. hominis strains isolated from different tissue sites showed quantitative differences in attachment to human widr (intest ...19873312097
electrophoretic analysis of proteins from mycoplasma hominis strains detected by sds-page, two-dimensional gel electrophoresis and immunoblotting.the proteins of 14 strains of mycoplasma hominis were compared by sds-page in gradient gels, by two-dimensional (2d) gel electrophoresis of extracts of 35s-labelled cells and by immunoblot analysis of cell proteins. the strains examined included the m. hominis type strain pg21 and 13 others isolated variously from genital tract, mouth, blood, upper urinary tract and a wound. these 14 strains shared 76-99% of proteins in sds-gradient gel analysis and 41-72% in the 2d gels. as expected, the immuno ...19873309143
[isolation of mycoplasma and chlamydia in patients with urogenital infections].vaginal/cervical swabs, catheter urine and/or puncture of the bladder (p.b.) from 880 females with urogenital diseases were examined microbiologically. mycoplasmas (ureaplasma urealyticum, mycoplasma hominis) could be isolated from 321 females (36.5%), and chlamydia trachomatis from 42 (4.8%). mycoplasmas and c. trachomatis were more frequent in the age between 21 and 30 years (53 and 11.8% respectively). in 32.8% of the entire collective mycoplasmas could be detected in vaginal swabs. in contra ...19873301631
nongenitourinary infections caused by mycoplasma hominis in adults.eleven new cases of mycoplasma hominis infection occurring outside the genitourinary tract in adults not in the puerperium were evaluated in relation to the 25 cases previously reported. cases of bacteremia (n = 14) were commonly associated with trauma or manipulation of the genitourinary tract and were often self-limited. wound infections (n = 14) followed surgery by 4-14 days in most cases and may have arisen from contamination from the genitourinary or respiratory tract. joint infections (n = ...19883293162
septic arthritis due to dual infection with mycoplasma hominis and ureaplasma urealyticum.acute septic arthritis of a knee and shoulder developed in a 32-year-old renal transplant patient. cultures yielded mycoplasma hominis and at least 1, and possibly 2, strains of ureaplasma urealyticum. doxycycline therapy controlled the symptoms and signs, and the joints became culture negative. on stopping therapy after 7 months, the arthritis recurred and u. urealyticum was again isolated from the shoulder joint. cessation of doxycycline almost 4 years after the initial episode resulted in ano ...19883283360
mycoplasma hominis septic arthritis.an unusual case is described of extragenital mycoplasma hominis infection presenting as a septic arthritis of the hip joint in a young paraplegic girl. the few reported cases of m. hominis septic arthritis are summarized. based on the current case and these reported cases, the etiology, presentation, diagnosis, and treatment of this infection are discussed.19883281546
ciprofloxacin treatment of chlamydial infections of urogenital tracts of women.ciprofloxacin was evaluated in chlamydial infections of the urogenital tracts of women treated with a dosage regimen of 500 mg orally twice a day for seven days. of the 40 women evaluated, 30 were infected with chlamydia trachomatis only, two were infected with neisseria gonorrhoeae only, and a further eight had combined gonococcal and chlamydial infections. ten were found to be harbouring chlamydia trachomatis in the urethra as well as the cervix. neisseria gonorrhoeae was eradicated from all p ...19883278970
chronic mycoplasma hominis infection complicating severe intraventricular hemorrhage, in a premature neonate. 19883231506
mycoplasma hominis meningitis in a premature infant. 19883231501
effect of blood culture media on the in vitro recovery of mycoplasma hominis.despite the prevalence of mycoplasma hominis few cases of septicaemia due to this organism have been reported. the ability of various blood culture media to sustain the growth of an inoculum of m. hominis was therefore studied. of the media tested, an 'in-house' hartley's digest broth with 0.1% glucose was the most efficient. the investigation also demonstrated that growth of m. hominis is adversely affected by the concentration of liquoid (sodium polyanetholsulphonate) present in blood culture ...19883216132
[genital infections and the course of pregnancy: a prospective study].the reported study investigates the relationship of genital infections, pathobiochemical findings and demographic data to preterm labor, premature rupture of membranes (prom) and premature delivery. the predictability of chorioamnionitis, puerperal and neonatal infections by these parameters was evaluated concurrently. 301 patients were included in this study between july 1985 and june 1986. 147 of these patients were studied longitudinally during pregnancy, delivery and puerperium (longitudinal ...19883215443
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethritis. vi. sensitivity of m. hominis to selected chemotherapeutic agents]. 19883210837
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethritis. v. sensitivity of u. urealyticum to selected chemotherapeutic agents]. 19883210836
effects of antimicrobial therapy on sperm-mucus interaction.sperm-mucus interaction under in-vitro or in-vivo conditions might be affected by microorganisms colonizing the reproductive tract. in order to study the influence of antimicrobial therapy, an extensive microbial screening was performed including chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, a broad spectrum of potentially pathogenic aerobic and anaerobic bacteria, trichomonas vaginalis, herpes simplex virus and yeasts. one-hundred-and-six couples with ...19883182976
microbiological profile of the cervix in 1,000 sexually active women.one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ...19883148302
[a case of peritonitis caused by mycoplasma hominis]. 19883143792
in vitro activity of florphenicol.florphenicol was active at a lower concentration than chloramphenicol against over half of 234 recent clinical bacterial isolates. the majority (98%) of the isolates were inhibited by florphenicol at a concentration of 8 mg/l or less. florphenicol was particularly effective against chloramphenicol resistant strains of haemophilus influenzae. klebsiella aerogenes and bacteroides spp. florphenicol was bacteristatic for salmonellae and escherichia coli but bactericidal for haemophilus influenzae. f ...19883143587
presence of anaplerotic reactions and transamination, and the absence of the tricarboxylic acid cycle in mollicutes.cell extracts of the fermentative mollicutes acholeplasma laidlawii b-pg9, acholeplasma morum s2, mycoplasma capricolum 14, mycoplasma gallisepticum s6, mycoplasma pneumoniae fh, mycoplasma hyopneumoniae j and m. genitalium g-37, and the non-fermentative mycoplasma hominis pg-21, mycoplasma hominis 1620 and mycoplasma bovigenitalium pg-11 were examined for 39 cytoplasmic enzyme activities associated with the tricarboxylic acid (tca) cycle, transamination, anaplerotic reactions and other enzyme a ...19883141576
gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra.in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ...19883140532
[isolation of human urogenital mycoplasma, mycoplasma hominis and ureaplasma urealyticum--comparative study between mycotrim-gu and conventional culture methods]. 19883137284
microbial aetiology and diagnostic criteria of postpartum endometritis in nairobi, kenya.using a protected triple lumen device, neisseria gonorrhoeae or chlamydia trachomatis, or both, were isolated from the endometriums of five out of 35 women with clinical postpartum endometritis compared with none of a control group of 30 puerperal women without endometritis (p less than 0.05) in nairobi, kenya. these sexually transmitted agents were also found in 12 cervical specimens from women with and three without postpartum endometritis (p = 0.04). mycoplasma hominis and ureaplasma urealyti ...19883137152
[in vitro activity of new quinolones against mycoplasma pathogenic to humans].the in vitro activity of new quinolones was evaluated against mycoplasma pneumoniae (10 strains) and mycoplasma hominis (approximately equal to 70 strains) by agar dilution, and against ureaplasma urealyticum (approximately equal to 115 strains) by broth dilution. the static effect of pefloxacin, ofloxacin, ciprofloxacin, enoxacin was investigated for all the strains. rosoxacin was included in the tests for u. urealyticum and m. hominis. pefloxacin, ofloxacin, ciprofloxacin and enoxacin were wit ...19883136430
lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology.we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ...19883135608
correlation of ultrasonography and bacteriology of the endocervix and posterior cul-de-sac of patients with severe pelvic inflammatory disease.we evaluated 30 patients admitted with a diagnosis of pelvic inflammatory disease (pid) by culdocentesis and ultrasonography to determine the severity of disease in relation to endocervical and culdocentesis bacteriology. specimens from the endocervix were cultured for neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum. in addition, culdocentesis fluid was cultured for aerobes and anaerobes. patients with n. gonorrhoeae or c. trachomatis had significantl ...19883135606
comparative in vitro activity of fleroxacin (ro 23-6240) against ureaplasma urealyticum and mycoplasma hominis.the susceptibility of 67 isolated ureaplasma urealyticum, 25 mycoplasma hominis and five mycoplasma spp. reference strains to fleroxacin, ofloxacin, josamycin and doxycycline was determined by agar dilution. josamycin had the best activity against ureaplasma urealyticum, followed in order by doxycycline, ofloxacin and fleroxacin. all the strains were inhibited by josamycin at 0.4 mg/l, while only 38.8% of the strains were inhibited by doxycycline at this concentration. mycoplasma hominis strains ...19883132382
non-occurrence of mycoplasma genitalium in clinical specimens.five hundred and thirteen clinical specimens, mainly from patients with urogenital inflammations, were examined for ureaplasma urealyticum and mycoplasmas, including cultures for mycoplasma genitalium. the study yielded 95 isolates of ureaplasma urealyticum, 37 isolates of mycoplasma hominis and two isolates of mycoplasma fermentans, but no growth of mycoplasma genitalium was obtained. it was concluded that mycoplasma genitalium is a relatively rare inhabitant of the human urogenital tract in is ...19883132377
prevalence of lower genital tract infections in pregnancy.a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ...19883128883
sexually transmittable organisms in the urethra of males with and without urethritis.in 589 males, 169 with and 420 without urethritis, urethral swabs were taken and assessed semiquantitatively for the sexually transmittable infectious agents neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, trichomonas vaginalis and candida species. the organisms were isolated in patients with and without urethritis as follows: n. gonorrhoeae with urethritis 19.5%, without 0.0%; c. trachomatis with urethritis 16.0%, without 2.9%; u. urealyticum (high cfu- ...19873118595
influence of microbial colonization on sperm-mucus interaction in vivo and in vitro.two-hundred-and-thirty-three asymptomatic couples with a mean duration of infertility of 5 years were submitted to postcoital testing (pt) and to sperm penetration meter test (spmt) and simultaneous microbial screening. cervical swabs and semen specimens were collected for culture of mycoplasma hominis, ureaplasma urealyticum, chlamydia trachomatis, neisseria gonorrhoeae, other potentially pathogenic and commensal aerobic and anaerobic bacteria, herpes simplex virus, vaginal swabs for trichomona ...19873114313
high-level tetracycline resistance in neisseria gonorrhoeae is result of acquisition of streptococcal tetm determinant.recently, strains of neisseria gonorrhoeae have been isolated which are highly resistant to tetracycline (mics of 16 to 64 micrograms/ml). this resistance was due to the acquisition of the resistance determinant tetm, a transposon-borne determinant initially found in the genus streptococcus and more recently in mycoplasma hominis, ureaplasma urealyticum, and gardnerella vaginalis. in n. gonorrhoeae, the tetm determinant was located on a 25.2-megadalton plasmid. this plasmid arose from the insert ...19863099640
in vitro activity of the two new 4-quinolones a56619 and a56620 against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum and gardnerella vaginalis.the in vitro activity of tetracycline, ciprofloxacin and two recently developed 1-aryl-fluoroquinolones, a56610 and a56620, was tested against 65 beta-lactamase-negative and 35 beta-lactamase-positive neisseria gonorrhoeae strains, 12 chlamydia trachomatis, 50 mycoplasma hominis, 28 ureaplasma urealyticum and 50 gardnerella vaginalis strains. in the case of chlamydia trachomatis and mycoplasma hominis both the mic and the mbc were determined. the mic90 of ciprofloxacin for neisseria gonorrhoeae ...19863096726
sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls.sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ...19863083395
epidemiologic and clinical characteristics of pelvic inflammatory disease associated with mycoplasma hominis, chlamydia trachomatis, and neisseria gonorrhoeae.we studied selected epidemiologic, clinical, serologic, and microbiologic findings and their interrelationships among 57 women with acute pelvic inflammatory disease (pid). cervical cultures positive for neisseria gonorrhoeae alone and for both n. gonorrhoeae and chlamydia trachomatis were associated with young age, nulliparity, and use of birth-control pills. positive serologic findings for c. trachomatis were associated with the isolation of c. trachomatis and/or n. gonorrhoeae from the cervix ...19863082018
the importance of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis infections in obstetrics and gynaecology. 19883075818
cervical chlamydia trachomatis and mycoplasmal infections in women with abnormal papanicolaou smears.in a series of 2,346 papanicolaou-stained smears from women undergoing routine gynaecological examination, 39 showed cytomorphological signs of inflammation suggesting chlamydia trachomatis infection (papanicolaou class ii or iii). the 39 smears were studied microbiologically by the direct-immunofluorescence test and cell culture to see whether chlamydial infection correlated with the presence of mycoplasma hominis and ureaplasma urealyticum. the results were compared with the cytological and co ...19883069248
mycoplasma hominis in maternal and fetal infections.although prevalence of m. hominis colonization during pregnancy varies from 12-50%, its role in infections of the mother and newborn infants is unclear. definite correlations exist with chorioamnionitis and amniotic fluid infections, but as it is rarely isolated alone during these infections, its pathogenic role is uncertain. its association with septic abortion is similarly questioned. prevalence and antibody titers to m. hominis increase with increasing parity. transient bacteremia occurs in a ...19883067641
protein antigens of genital mycoplasmas.surface structures of the genital mycoplasmas ureaplasma urealyticum and mycoplasma hominis that are important in the human immune response and pathogenesis of disease are relatively poorly defined. in this study, an unusual antigen complex of u. urealyticum consisting of multiple bands forming a "ladder" pattern after electrophoretic separation was noted. it is similar to the variable v-1 surface antigen of mycoplasma pulmonis. data on u. urealyticum are only preliminary, but the ureaplasma ant ...19883055206
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