Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| enterococcus mundtii st4sa and lactobacillus plantarum 423 excludes listeria monocytogenes from the git, as shown by bioluminescent studies in mice. | listeria monocytogenes is an opportunistic food-borne pathogen and is life-threatening to individuals with a weakened immune system. the aim of this study was to determine if lactobacillus plantarum 423 and enterococcus mundtii st4sa could prevent colonisation of l. monocytogenes in the gastro-intestinal tract (git). mice were gavaged with l. plantarum 423, e. mundtii st4sa, and a combination of the two strains, for 6 consecutive days and orally infected with a bioluminescent strain of l. monocy ... | 2016 | 26689230 |
| prophage-mediated modulation of interaction of streptococcus thermophilus j34 with human intestinal epithelial cells and its competition against human pathogens. | the human intestinal microbiota plays an important role in human health. while adhesion to gastrointestinal mucosa is a prerequisite for colonisation, inhibition of adhesion is a property which may prevent or reduce infections by food borne pathogens. streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus represent the two lactic bacteria constituting the yoghurt culture. these starter cultures have been claimed to be probiotic. in our study we compared two s. thermophilus st ... | 2016 | 26689226 |
| cutting edge: novel tmem173 allele reveals importance of sting n terminus in trafficking and type i ifn production. | with the stimulator of ifn genes (sting) c terminus being extensively studied, the role of the n-terminal domain (ntd) of sting remains an important subject of investigation. in this article, we identify novel mutations in ntd of sting of the molf strain in response to hsv and listeria monocytogenes both in vitro and in vivo. these mutations are responsible for low levels of ifn-β caused by failure of molf sting to translocate from the endoplasmic reticulum. these data provide evidence that the ... | 2016 | 26685207 |
| tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m). | tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ... | 2016 | 26682961 |
| listeriosis phytotherapy: a review study on the effectiveness of iranian medicinal plants in treatment of listeriosis. | listeria monocytogenes can be found in many processed foods, raw milk, dairy products, meat and meat products such as sausages, beef and fish products, seafoods, eggs, fruits, and vegetables such as radish and cabbage. this article is a review study on the iranian medicinal plants applied for treatment of listeriosis. information of this review article was obtained by searching various key words such as listeria monocytogenes, medicinal plants, plant extracts and essential oils among scientific ... | 2015 | 26682768 |
| long-term exposure to high levels of decabrominated diphenyl ether inhibits cd4 t-cell functions in c57bl/6 mice. | in recent years, the adverse health effects of decabrominated diphenyl ether (bde-209) have raised more concerns as a growing number of studies reported its persistence in the environment and abundance in the human population, especially in occupational environmental compartments and exposed personnel. this study applies our previous animal model simulating occupational exposure to bde-209 to investigate its potential adverse effects on cd4 t cells. female c57bl/6 mice were orally gavaged with b ... | 2016 | 26682527 |
| proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases. | bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ... | 2015 | 26682199 |
| complete genome sequences of two listeria monocytogenes serovars, 1/2a and 4b, isolated from dairy products in brazil. | listeria monocytogenes is the foodborne pathogen responsible for a bacterial infection called listeriosis. here, we present the whole-genome sequences of two l. monocytogenes serovars, 1/2a and 4b, which are considered the most prevalent in food processing plants and listeriosis outbreaks, respectively. | 2015 | 26679596 |
| inactivation kinetics of listeria monocytogenes and salmonella enterica serovar typhimurium on fresh-cut bell pepper treated with slightly acidic electrolyzed water combined with ultrasound and mild heat. | the goal of this study was to enhance the antimicrobial effect of slightly acidic electrolyzed water (saew) through addition of synergistic treatment with ultrasound (us) and mild heat treatment in order to improve the microbial safety of fresh-cut bell pepper. to evaluate the synergistic effects, the weibull model was used to mathematically measure the effectiveness of the individual and combined treatments against listeria monocytogenes and salmonella typhimurium on the pepper. the combined tr ... | 2016 | 26678144 |
| the challenge of enumerating listeria monocytogenes in food. | listeria monocytogenes is recognised as a serious foodborne pathogen in humans. however, food products are usually contaminated at low levels (i.e. <100 cfu/g) and there is still no adequate enumeration method for testing food. much research has been carried out to improve listeria enumeration methods, leading to several proposed alternative methods such as the most probable number technique, molecular-based methods and bacterial cell concentration techniques. here, we catalogue the current know ... | 2016 | 26678141 |
| development of a model describing the inhibitory effect of selected preservatives on the growth of listeria monocytogenes in a meat model system. | the objective of this study was to evaluate the impact of seven independent factors consisting of sodium nitrite, ph, sodium chloride, sodium acetate, sodium lactate syrup, calcium propionate and a blend of nisin and hop alpha acids on the growth rate of listeria monocytogenes in ham as a model of ready-to-eat (rte) meat products. a central composite consisted of seven factors mentioned above was designed and the response surface methodology was applied for creating a mathematic model to predict ... | 2016 | 26678138 |
| antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products. | lactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolates belon ... | 2015 | 26675908 |
| [a clinical analysis of 16 patients with maternal listeriosis]. | to summarize the clinical characteristics and outcome of maternal listeriosis so as to improve the understanding of disease and the level of diagnosis and treatment. | 2015 | 26674793 |
| impact of t300a variant of atg16l1 on antibacterial response, risk of culture positive infections, and clinical course of crohn's disease. | autophagy-related 16-like 1 (atg16l1) deficiency leads to impaired cellular autophagy and bacterial degradation as well as an altered cytokine production. the single-nucleotide polymorphism rs2241880 (t300a) is associated with an increased risk for crohn's disease (cd). atg16l1 polymorphisms could therefore have an impact on the risk of infectious complications and disease course in cd. we examined the impact of the t300a genotype on the antibacterial response toward a panel of pathogenic bacter ... | 2015 | 26673830 |
| rhombencephalitis due to listeria monocytogenes: a clinicopathologic study of a case. | central nervous system infections caused by listeria monocytogenes usually manifest in the form of meningitis or meningoencephalitis, and are more common among immunosuppressed patients. brainstem encephalitis (rhombencephalitis) is less common and fatal if not recognized and treated early. | 2015 | 26671740 |
| listeriolysin o, but not murine e-cadherin, is involved in invasion of listeria monocytogenes into murine liver parenchymal cells. | human e-cadherin and listeriolysin o (llo) are involved in invasion of listeria monocytogenes into human liver parenchymal cells (lpc). yet, it remains to be determined whether murine e-cadherin and llo participate in invasion of l. monocytogenes into murine lpc. in the present study, involvement of murine e-cadherin and llo in invasion of l. monocytogenes into murine lpc was investigated. murine e-cadherin was expressed on murine lpc, but the expression became undetectable by insertion of trans ... | 2015 | 26668665 |
| detection of virulence genes and growth potential in listeria monocytogenes strains isolated from ricotta salata cheese. | ricotta salata is a traditional ripened and salted whey cheese made in sardinia (italy) from sheep's milk. this product is catalogued as ready-to-eat food (rte) since it is not submitted to any further treatment before consumption. thus, foodborne pathogens, such as listeria monocytogenes, can represent a health risk for consumers. in september 2012, the fda ordered the recall of several batches of ricotta salata imported from italy linked to 22 cases of listeriosis in the united states. this st ... | 2016 | 26666835 |
| in vitro evaluation of bacteriocins activity against listeria monocytogenes biofilm formation. | the present study aimed to assess the activity of cell-free supernatant (cfs) containing bacteriocins on the formation and maintenance of biofilms developed by listeria monocytogenes, and the associated effect of bacteriocins and ethylene-diamine-tetra-acetic acid (edta) on the formed biofilm. cfs from 9 lactic acid bacteria (lab) strains was tested for inhibitory activity against 85 l. monocytogenes isolates and 21 lab strains. then, 12 l. monocytogenes strains were selected based on genetic pr ... | 2016 | 26660467 |
| pore-forming toxins induce macrophage necroptosis during acute bacterial pneumonia. | necroptosis is a highly pro-inflammatory mode of cell death regulated by rip (or ripk)1 and rip3 kinases and mediated by the effector mlkl. we report that diverse bacterial pathogens that produce a pore-forming toxin (pft) induce necroptosis of macrophages and this can be blocked for protection against serratia marcescens hemorrhagic pneumonia. following challenge with s. marcescens, staphylococcus aureus, streptococcus pneumoniae, listeria monocytogenes, uropathogenic escherichia coli (upec), a ... | 2015 | 26659062 |
| molecular analysis of dominant species in listeria monocytogenes-positive biofilms in the drains of food processing facilities. | listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. in this study, we investigated l. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species of the biofilms were identified to determine the properties of the microbiological background. for this purpose, the iso 11290 method was used for the detection and isolation of l. m ... | 2016 | 26658820 |
| comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria. | the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ... | 2015 | 26656394 |
| real-time investigation of antibiotics-induced oxidative stress and superoxide release in bacteria using an electrochemical biosensor. | the involvement of oxidative stress in the mechanism of antibiotics-meditated cell death is unclear and subject to debate. the kinetic profile and a quantitative relationship between the release of reactive oxygen species (ros), bacteria and antibiotic type remain elusive. here we report direct measurements and analytical quantification of the release of superoxide radicals (o2(·-)), a major contributor to ros, in antibiotics-treated bacterial cultures using a cytochrome c electrochemical biosen ... | 2016 | 26655038 |
| biofabrication of zinc oxide nanoparticles using fruit extract of rosa canina and their toxic potential against bacteria: a mechanistic approach. | the use of plant extract in the biosynthesis of nanoparticles (nps) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. in this study, the biosynthesis of zinc oxide (zno) nps by both "conventional heating" (ch) and "microwave irradiation" (mi) methods has been reported. stable and spherical znonps were produced using zinc nitrate and flesh extract of rosa canina fruit (rosehip) which was used as a precurso ... | 2016 | 26652376 |
| sensitive detection of campylobacter jejuni using nanoparticles enhanced qcm sensor. | a quartz crystal microbalance (qcm) sensor platform was used to develop an immunosensor for the detection of food pathogen campylobacter jejuni. rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against c. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (aunps) amplified sandwich assays. the performance of the qcm immunosensor developed using sandwich assay by utilising the rabbit polyclonal antibody as the capture antibody and conj ... | 2016 | 26649490 |
| in vitro assessment of the antimicrobial potentials of lactobacillus helveticus strains isolated from traditional cheese in sinkiang china against food-borne pathogens. | lactobacillus helveticus, an obligatory hetero-fermentative lab, is generally recognized as safe (gras) and is gaining popularity for application in dairy products. lactic acid bacteria (lab) play a remarkable role in inhibiting the growth of pathogenic bacteria in food products, without disturbing the sensory attributes of the food. in this study, the screening of the antimicrobial potential of lactobacillus helveticus klds 1.8701 against four food-borne pathogens including listeria monocytogen ... | 2016 | 26648272 |
| chemical composition and antibacterial activity of some medicinal plants from lamiaceae family. | chemical composition and antibacterial activity of aqueous (ethanolic and methanolic) extracts from herbs often used in polish cuisine and traditional herbal medicine including thyme (thymus vulgaris l.), rosemary (rosmarinus officinalis l.), oregano (origanum vulgare l.), peppermint (mentha piperita l.) and sage (salvia officinalis l.) were compared. the aqueous ethanolic extracts contained slightly higher levels of phenolics compared to the aqueous methanolic extracts. in turn, gc-ms analysis ... | 2015 | 26647633 |
| plasmonic enzyme-linked immunosorbent assay using nanospherical brushes as a catalase container for colorimetric detection of ultralow concentrations of listeria monocytogenes. | plasmonic enzyme-linked immunosorbent assay (pelisa) based on catalase (cat)-mediated gold nanoparticle growth exhibits ultrahigh sensitivity for detecting disease-related biomarkers using sandwich formats. however, the limit of detection (lod) of this strategy for listeria monocytogenes is only around 10(3) cfu/ml, which considerably exceeds the amount of l. monocytogenes commonly present in food products (<100 cfu/g). herein, we report an improved pelisa method for detection of l. monocytogene ... | 2015 | 26646325 |
| listeria monocytogenes bacteremia in a pancreas transplant recipient. | we report a case of listeria monocytogenes bacteremia in a patient 12 years after his pancreas transplant, during which time he received a steroid-free immunosuppressive regimen. to our knowledge, there are no reported cases describing l monocytogenes bacteremia after pancreas transplant. in addition, although typically identified as a complication shortly after transplant or after treatment for organ rejection, this case demonstrates that it is still possible for a patient to develop a l monocy ... | 2015 | 26645926 |
| interleukin-22-induced antimicrobial phospholipase a2 group iia mediates protective innate immunity of nonhematopoietic cells against listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen which establishes intracellular parasitism in various cells, including macrophages and nonhematopoietic cells, such as hepatocytes. it has been reported that several proinflammatory cytokines have pivotal roles in innate protection against l. monocytogenes infection. we found that a proinflammatory cytokine, interleukin 22 (il-22), was expressed by cd3(+) cd4(+) t cells at an early stage of l. monocytogenes infection in mice. to assess the influence ... | 2015 | 26644377 |
| choice of reference-guided sequence assembler and snp caller for analysis of listeria monocytogenes short-read sequence data greatly influences rates of error. | the influences that different programs and conditions have on error rates of single-nucleotide polymorphism (snp) analyses are poorly understood. using illumina short-read sequence data generated from listeria monocytogenes strain hpb5622, we assessed the performance of four snp callers (bcftools, freebayes, unifiedgenotyper, varscan) under a variety of conditions, including: (1) a range of sequencing coverages; (2) use of four popular reference-guided assemblers (burrows-wheeler aligner, novoal ... | 2015 | 26643440 |
| biosynthesis of paenibacillin, a lantibiotic with n-terminal acetylation, by paenibacillus polymyxa. | paenibacillin, a recently-discovered lantibiotic from paenibacillus polymyxa osy-df, showed potency against listeria monocytogenes, methicillin-resistant staphylococcus aureus and other gram-positive bacteria. the chemical structure of paenibacillin has been determined previously. this study was initiated to investigate the biosynthesis of paenibacillin, and to reveal unique features in its biosynthetic pathway. paenibacillin structural gene (paea) was identified by polymerase chain reaction (pc ... | 2015 | 26640048 |
| to modulate survival under secondary stress conditions, listeria monocytogenes 10403s employs rsbx to downregulate σb activity in the poststress recovery stage or stationary phase. | listeria monocytogenes is a saprophytic bacterium that thrives in diverse environments and causes listeriosis via ingestion of contaminated food. rsbx, a putative sigma b (σ(b)) regulator, is thought to maintain the ready state in the absence of stress and reset the bacterium to the initial state in the poststress stage in bacillus subtilis. we wondered whether rsbx is functional in l. monocytogenes under different stress scenarios. genetic deletion and complementation of the rsbx gene were comb ... | 2015 | 26637594 |
| restraining of reactive oxygen species promotes invasion of listeria monocytogenes into glia cells. | listeria monocytogenes is a foodborne pathogen that could cause severe infection in the central nervous system of humans and animals. however, the molecular mechanism of the pathogenesis is not fundamentally assessed. this study aimed to analyze the role of reactive oxygen species (ros) in l. monocytogenes during its invasion into glia cells. the ros level in l. monocytogenes was manipulated using nad(p)h oxidase inhibitor diphenyleneiodonium chloride (dpi) and ros scavenger n-acetyl cysteine (n ... | 2016 | 26635415 |
| the novel multiple inner primers-loop-mediated isothermal amplification (mip-lamp) for rapid detection and differentiation of listeria monocytogenes. | here, a novel model of loop-mediated isothermal amplification (lamp), termed multiple inner primers-lamp (mip-lamp), was devised and successfully applied to detect listeria monocytogenes. a set of 10 specific mip-lamp primers, which recognized 14 different regions of target gene, was designed to target a sequence in the hlya gene. the mip-lamp assay efficiently amplified the target element within 35 min at 63 °c and was evaluated for sensitivity and specificity. the templates were specially ampl ... | 2015 | 26633345 |
| [infectious diseases in the genomic era]. | next generation sequencing (ngs) technologies have arrived, changing research and infectious disease research into a new era, the "genomic era". currently, the developed world is introducing ngs in a number of applications, including clinical diagnostics, epidemiology, and microbiology. in developing countries ngs is being progressively introduced. technologies currently available allow to sequence the whole genome of bacterial and viral strains for an approximate cost of $100 usd, which is high ... | 2015 | 26633116 |
| teichoic acid is the major polysaccharide present in the listeria monocytogenes biofilm matrix. | the aim of this study was to characterize the listeria monocytogenes biofilm and particularly the nature of the carbohydrates in the biofilm extracellular matrix and culture supernatant versus to cell wall carbohydrates. listeria monocytogenes serotype 1/2a and 4b strains were able to form complex biofilms embedded in an extracellular matrix. the soluble carbohydrates from biofilm extracellular matrix and culture supernatant were identified as teichoic acids, structurally identical to cell wall ... | 2016 | 26626878 |
| [sanitary significance of soil sea coasts]. | there was investigated the dynamics of growth of listeria monocytogenes and yersinia pseudotuberculosis in soils of sea coast (mid-flight and maritime soils). these bacteria were shown to reproduced well in all researched soils, preferring nevertheless maritime soils. the content of the humus was determined to be the one of the limiting factors restricting the multiplication of pathogenic bacteria in studied soils. abiotic characteristic of soils of sea coast were established to render the direc ... | 2015 | 26625609 |
| prevalence of listeria monocytogenes in raw bovine milk and milk products from central highlands of ethiopia. | listeria monocytogenes is of major significance in human and veterinary medicine. most human listeria infections are foodborne and the association of contaminated milk and dairy produce consumption with human listeriosis is noteworthy. in ethiopia, there is limited data regarding the prevalence of l. monocytogenes in raw bovine milk and dairy products. the aim of this study was, therefore, to determine the prevalence of l. monocytogenes in raw bovine milk and dairy produce. | 2015 | 26623629 |
| listeria infected pseudoaneurysm of the superficial femoral artery. | we present a unique case of listeria-infected pseudoaneurysm of the superficial femoral artery. listeria monocytogenes, a gram positive, intracellular bacterium, is widespread in the environment. the manifestation of infection in humans ranges from mild gastroenteritis to life-threatening listeriosis. the incidence in the healthy population is low (0.7 per 100,000) but rises sharply in pregnancy, the immunocompromised and patients with malignancy. listeria rarely causes mycotic aneurysm formatio ... | 2016 | 26620380 |
| zebrafish: an animal model for research in veterinary medicine. | the zebrafish (danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their devel ... | 2015 | 26618602 |
| systemic listeriosis following vaccination with the attenuated listeria monocytogenes therapeutic vaccine, adxs11-001. | 2016 | 26618528 | |
| bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions. | clove oil (co) anionic nanoemulsions were prepared with varying ratios of co to canola oil (ca), emulsified and stabilized with purity gum ultra (pgu), a newly developed succinylated waxy maize starch. interfacial tension measurements showed that co acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5:5% v/v co:ca) was reached. antimicrobial activity of the negatively charge ... | 2016 | 26616926 |
| an invertebrate signal transducer and activator of transcription 5 (stat5) ortholog from the disk abalone, haliotis discus discus: genomic structure, early developmental expression, and immune responses to bacterial and viral stresses. | signal transducer and activator of transcription (stat) family members are key signaling molecules that transduce cellular responses from the cell membrane to the nucleus upon janus kinase (jak) activation. although seven stat members have been reported in mammals, very limited information on stat genes in molluscans is available. in this study, we identified and characterized a stat paralog that is homologous to stat5 from the disk abalone, haliotis discus discus, and designated as abstat5. com ... | 2016 | 26616564 |
| a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens. | galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ... | 2016 | 26615008 |
| microbial dynamics in mixed culture biofilms of bacteria surviving sanitation of conveyor belts in salmon-processing plants. | the microbiota surviving sanitation of salmon-processing conveyor belts was identified and its growth dynamics further investigated in a model mimicking processing surfaces in such plants. | 2016 | 26613979 |
| low temperature and modified atmosphere: hurdles for antibiotic resistance transfer? | food is an important dissemination route for antibiotic-resistant bacteria. factors used during food production and preservation may contribute to the transfer of antibiotic resistance genes, but research on this subject is scarce. in this study, the effect of temperature (7 to 37°c) and modified atmosphere packaging (air, 50% co2-50% n2, and 100% n2) on antibiotic resistance transfer from lactobacillus sakei subsp. sakei to listeria monocytogenes was evaluated. filter mating was performed on no ... | 2015 | 26613914 |
| examination of listeria monocytogenes in seafood processing facilities and smoked salmon in the republic of ireland. | listeria monocytogenes is a foodborne pathogen that causes listeriosis, a relatively rare but life-threatening disease primarily affecting immunocompromised individuals. the aim of this study was to determine the prevalence of l. monocytogenes in the seafood processing industry in the republic of ireland. the occurrence of l. monocytogenes was determined by regular sampling of both food samples and processing environment swabs at eight seafood processing facilities over two calendar years. all s ... | 2015 | 26613913 |
| extending rad tag analysis to microbial ecology: a comparison between multilocus sequence typing and 2b-rad to investigate listeria monocytogenes genetic structure. | the advent of next-generation sequencing (ngs) has dramatically changed bacterial typing technologies, increasing our ability to differentiate bacterial isolates. despite it is now possible to sequence a bacterial genome in a few days and at reasonable costs, most genetic analyses do not require whole-genome sequencing, which also remains impractical for large population samples due to the cost of individual library preparation and bioinformatics. more traditional sequencing approaches, however, ... | 2016 | 26613186 |
| the effect of nacl reduction in the microbiological quality of cracked green table olives of the maçanilha algarvia cultivar. | the present work aimed at studying the effect of the partial replacement of nacl with kcl and cacl2 of the fermenting brines on the microbiological quality of natural cracked green maçanilha algarvia table olives. olives were fermented in different salt combinations (brine 1-8% nacl, brine 2-4% nacl 4% kcl, brine 3-4% nacl 4% cacl2, brine 4-4% kcl 4% cacl2, and brine 5-2.7% nacl 2.7% kcl 2.7% cacl2) and the abundance of yeasts and enterobacteria was determined. at the end of fermentation, the ma ... | 2016 | 26613162 |
| effect of salt types and concentrations on the high-pressure inactivation of listeria monocytogenes in ground chicken. | national and international health agencies have recommended a significant reduction in daily intake of sodium by reducing the amount of nacl in foods, specifically processed meats. however, sodium reduction could increase the risk of survival and growth of spoilage and pathogenic microorganisms on these products. therefore, alternate processing technologies to improve safety of sodium reduced foods are necessary. this study examined the effects of three different salt types and concentrations on ... | 2016 | 26613161 |
| inhibition mechanism of listeria monocytogenes by a bioprotective bacteria lactococcus piscium cncm i-4031. | listeria monocytogenes is a pathogenic gram positive bacterium and the etiologic agent of listeriosis, a severe food-borne disease. lactococcus piscium cncm i-4031 has the capacity to prevent the growth of l. monocytogenes in contaminated peeled and cooked shrimp. to investigate the inhibititory mechanism, a chemically defined medium (msma) based on shrimp composition and reproducing the inhibition observed in shrimp was developed. in co-culture at 26 °c, l. monocytogenes was reduced by 3-4 log ... | 2016 | 26611171 |
| aminopeptidase t of m29 family acts as a novel intracellular virulence factor for listeria monocytogenes infection. | the foodborne pathogen listeria monocytogenes employs a number of virulence determinants including metalloproteases to infect hosts. here for the first time, we identified an m29 family aminopeptidase t (encoded by lmo1603) from l. monocytogenes that possesses a typical feature to catalyze the cleavage of amino acids from peptide substrates, with a preference for arginine. the purified recombinant lmo1603 was activated by fe(3+), zn(2+) and mn(2+), but strongly stimulated by co(2+), indicating t ... | 2015 | 26610705 |
| prospective whole-genome sequencing enhances national surveillance of listeria monocytogenes. | whole-genome sequencing (wgs) has emerged as a powerful tool for comparing bacterial isolates in outbreak detection and investigation. here we demonstrate that wgs performed prospectively for national epidemiologic surveillance of listeria monocytogenes has the capacity to be superior to our current approaches using pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst), multilocus variable-number tandem-repeat analysis (mlva), binary typing, and serotyping. initially 423 l. ... | 2016 | 26607978 |
| effect of atmospheric pressure plasma jet on the foodborne pathogens attached to commercial food containers. | bacterial biofilms are associated with numerous infections and problems in the health care and food industries. the aim of this study was to evaluate the bactericidal effect of an atmospheric pressure plasma (app) jet on escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium biofilm formation on collagen casing (cc), polypropylene (pp) and polyethylene terephthalate (pet), which are widely used food container materials. the samples were treated separately with the app jet a ... | 2015 | 26604423 |
| in-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates. | protein hydrolysates were prepared from chicken liver using fermentation and enzymatic hydrolysis. the lactic acid bacteria pediococcus acidilactici ncim5368 was employed in the fermentation process and a commercial protease (alcalase® 2.5) was used in enzymatic hydrolysis. chicken liver hydrolysates prepared by fermentation (fclh) and enzymatic hydrolysis (eclh) revealed appreciable amounts of protein [55.85 and 61.34 %; on dry weight basis, respectively]. fermentation and enzymatic hydrolysis ... | 2015 | 26604378 |
| rapid identification and classification of listeria spp. and serotype assignment of listeria monocytogenes using fourier transform-infrared spectroscopy and artificial neural network analysis. | the use of fourier transform-infrared spectroscopy (ft-ir) in conjunction with artificial neural network software neurodeveloper™ was examined for the rapid identification and classification of listeria species and serotyping of listeria monocytogenes. a spectral library was created for 245 strains of listeria spp. to give a biochemical fingerprint from which identification of unknown samples were made. this technology was able to accurately distinguish the listeria species with 99.03% accuracy. ... | 2015 | 26600423 |
| dynamics of listeria monocytogenes colonisation in a newly-opened meat processing facility. | this study determined the colonisation scenario of listeria monocytogenes in a newly-opened ready-to-eat meat processing facility using a combination of classical microbiology and molecular biology techniques. samples (n=183), including food contact surfaces, non-food contact surfaces, raw materials and food samples, collected on four sampling occasions, were analysed for l. monocytogenes by the iso 11290:1996 standard method and by real-time pcr applied to the second enrichment broth from the i ... | 2016 | 26599913 |
| risk factors for sporadic non-pregnancy associated listeriosis in germany-immunocompromised patients and frequently consumed ready-to-eat products. | non-pregnancy associated (n-pa) listeriosis, caused by listeria monocytogenes, is a rare but severe disease, and is predominantly food-borne. most cases appear sporadic and their infection vehicle remains unknown. incidence has increased since 2008 in germany. we aimed to identify underlying conditions and foods associated with sporadic n-pa listeriosis in germany. we performed a nationwide case-control study from march 2012-december 2013. cases were sporadic n-pa listeriosis patients notified t ... | 2015 | 26599484 |
| collapsin response mediator protein-1 regulates arp2/3-dependent actin assembly. | listeria monocytogenes is a bacterial parasite that uses host proteins to assemble an arp2/3-dependent actin comet tail to power its movement through the host cell. initiation of comet tail assembly is more efficient in cytosol than it is under defined conditions, indicating that unknown factors contribute to the reaction. we therefore fractionated cytosol and identified crmp-1 as a factor that facilitates arp2/3-dependent listeria actin cloud formation in the presence of arp2/3 and actin alone. ... | 2016 | 26598519 |
| exoproteome analysis reveals higher abundance of proteins linked to alkaline stress in persistent listeria monocytogenes strains. | the foodborne pathogen listeria monocytogenes, responsible for listeriosis a rare but severe infection disease, can survive in the food processing environment for month or even years. so-called persistent l. monocytogenes strains greatly increase the risk of (re)contamination of food products, and are therefore a great challenge for food safety. however, our understanding of the mechanism underlying persistence is still fragmented. in this study we compared the exoproteome of three persistent st ... | 2016 | 26594790 |
| tolerance of listeria monocytogenes to quaternary ammonium sanitizers is mediated by a novel efflux pump encoded by emre. | a novel genomic island (lgi1) was discovered in listeria monocytogenes isolates responsible for the deadliest listeriosis outbreak in canada, in 2008. to investigate the functional role of lgi1, the outbreak strain 08-5578 was exposed to food chain-relevant stresses, and the expression of 16 lgi1 genes was measured. lgi1 genes with putative efflux (l. monocytogenes emre [emrelm]), regulatory (lmo1851), and adhesion (sel1) functions were deleted, and the mutants were exposed to acid (hcl), cold ( ... | 2015 | 26590290 |
| determination of evolutionary relationships of outbreak-associated listeria monocytogenes strains of serotypes 1/2a and 1/2b by whole-genome sequencing. | we used whole-genome sequencing to determine evolutionary relationships among 20 outbreak-associated clinical isolates of listeria monocytogenes serotypes 1/2a and 1/2b. isolates from 6 of 11 outbreaks fell outside the clonal groups or "epidemic clones" that have been previously associated with outbreaks, suggesting that epidemic potential may be widespread in l. monocytogenes and is not limited to the recognized epidemic clones. pairwise comparisons between epidemiologically related isolates wi ... | 2015 | 26590286 |
| validation of a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in new york state produce fields. | technological advancements, particularly in the field of geographic information systems (gis), have made it possible to predict the likelihood of foodborne pathogen contamination in produce production environments using geospatial models. yet, few studies have examined the validity and robustness of such models. this study was performed to test and refine the rules associated with a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in produce farms in n ... | 2015 | 26590280 |
| processing-dependent and clonal contamination patterns of listeria monocytogenes in the cured ham food chain revealed by genetic analysis. | the quantitative and qualitative patterns of environmental contamination by listeria monocytogenes were investigated in the production chain of dry-cured parma ham. standard arrays of surfaces were sampled in processing facilities during a single visit per plant in the three compartments of the food chain, i.e., ham production (19 plants) and postproduction, which was divided into deboning (43 plants) and slicing (25 plants) steps. the numbers of sampled surfaces were 384 in ham production, with ... | 2015 | 26590278 |
| listeria monocytogenes: strain heterogeneity, methods, and challenges of subtyping. | listeria monocytogenes is a food-borne bacterial pathogen that is associated with 20% to 30% case fatality rate. l. monocytogenes is a genetically heterogeneous species, with a small fraction of strains (serotypes 1/2a, 1/2b, 4b) implicated in human listeriosis. monitoring and source tracking of l. monocytogenes involve the use of subtyping methods, with the performance of genetic-based methods found to be superior to phenotypic-based ones. various methods have been used to subtype l. monocytoge ... | 2015 | 26588067 |
| smad4 represses the generation of memory-precursor effector t cells but is required for the differentiation of central memory t cells. | the transcriptional regulation underlying the differentiation of cd8(+) effector and memory t cells remains elusive. here, we show that 18-month-old mice lacking the transcription factor smad4 (homolog 4 of mothers against decapentaplegic, drosophila), a key intracellular signaling effector for the tgf-β superfamily, in t cells exhibited lower percentages of cd44(hi)cd8(+) t cells. to explore the role of smad4 in the activation/memory of cd8(+) t cells, 6- to 8-week-old mice with or without smad ... | 2015 | 26583325 |
| economic cost of a listeria monocytogenes outbreak in canada, 2008. | estimates of the economic costs associated with foodborne disease are important to inform public health decision-making. in 2008, 57 cases of listeriosis and 24 deaths in canada were linked to contaminated delicatessen meat from one meat processing plant. costs associated with the cases (including medical costs, nonmedical costs, and productivity losses) and those incurred by the implicated plant and federal agencies responding to the outbreak were estimated to be nearly $242 million canadian do ... | 2015 | 26583272 |
| health trajectories reveal the dynamic contributions of host genetic resistance and tolerance to infection outcome. | resistance and tolerance are two alternative strategies hosts can adopt to survive infections. both strategies may be genetically controlled. to date, the relative contribution of resistance and tolerance to infection outcome is poorly understood. here, we use a bioluminescent listeria monocytogenes (lm) infection challenge model to study the genetic determination and dynamic contributions of host resistance and tolerance to listeriosis in four genetically diverse mouse strains. using convention ... | 2015 | 26582028 |
| the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens. | the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ... | 2015 | 26580308 |
| an insight into the isolation, enumeration, and molecular detection of listeria monocytogenes in food. | listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. the ability of l. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. this review provides insight into the methods for cultural detection, enumeration, and molecular identification of l ... | 2015 | 26579116 |
| a detailed view of the intracellular transcriptome of listeria monocytogenes in murine macrophages using rna-seq. | listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which is mainly a threat for pregnant, elderly, or immunocompromised individuals. due to its ability to invade and colonize diverse eukaryotic cell types including cells from invertebrates, l. monocytogenes has become a well-established model organism for intracellular growth. almost 10 years ago, we and others presented the first whole-genome microarray-based intracellular transcriptome o ... | 2015 | 26579105 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| growth and inactivation of salmonella enterica and listeria monocytogenes in broth and validation in ground pork meat during simulated home storage abusive temperature and home pan-frying. | ground pork meat with natural microbiota and inoculated with low initial densities (1-10 or 10-100 cfu/g) of salmonella enterica or listeria monocytogenes was stored under abusive temperature at 10°c and thermally treated by a simulated home pan-frying procedure. the growth and inactivation characteristics were also evaluated in broth. in ground pork meat, the population of s. enterica increased by less than one log after 12-days of storage at 10°c, whereas l. monocytogenes increased by 2.3 to 2 ... | 2015 | 26579079 |
| structure of the bacterial cell division determinant gpsb and its interaction with penicillin-binding proteins. | each bacterium has to co-ordinate its growth with division to ensure genetic stability of the population. consequently, cell division and growth are tightly regulated phenomena, albeit different bacteria utilise one of several alternative regulatory mechanisms to maintain control. here we consider gpsb, which is linked to cell growth and division in gram-positive bacteria. δgpsb mutants of the human pathogen listeria monocytogenes show severe lysis, division and growth defects due to distortions ... | 2016 | 26575090 |
| inflammasomes coordinate pyroptosis and natural killer cell cytotoxicity to clear infection by a ubiquitous environmental bacterium. | defective neutrophils in patients with chronic granulomatous disease (cgd) cause susceptibility to extracellular and intracellular infections. microbes must first be ejected from intracellular niches to expose them to neutrophil attack, so we hypothesized that inflammasomes detect certain cgd pathogens upstream of neutrophil killing. here, we identified one such ubiquitous environmental bacterium, chromobacterium violaceum, whose extreme virulence was fully counteracted by the nlrc4 inflammasome ... | 2015 | 26572063 |
| autophagy regulates t lymphocyte proliferation through selective degradation of the cell-cycle inhibitor cdkn1b/p27kip1. | the highly conserved cellular degradation pathway, macroautophagy, regulates the homeostasis of organelles and promotes the survival of t lymphocytes. previous results indicate that atg3-, atg5-, or pik3c3/vps34-deficient t cells cannot proliferate efficiently. here we demonstrate that the proliferation of atg7-deficient t cells is defective. by using an adoptive transfer and listeria monocytogenes (lm) mouse infection model, we found that the primary immune response against lm is intrinsically ... | 2015 | 26569626 |
| a delayed cerebral vasospasm with infarction is secondary to listeria monocytogenes meningitis: mri and mra are diagnostically useful. | among patients with bacterial meningitis, a cerebral vasospasm typically occurs during the acute phase. we experienced a case of delayed cerebral vasospasm with infarction that was secondary to listeria monocytogenes meningitis. an 82-year-old woman with listeria monocytogenes meningitis, whose symptoms had been improving after the initiation of antibacterial therapy, fell into a coma on day 15 and developed generalized seizure. magnetic resonance imaging (mri) and mr angiography (mra) indicated ... | 2015 | 26568013 |
| pectin and xyloglucan influence the attachment of salmonella enterica and listeria monocytogenes to bacterial cellulose-derived plant cell wall models. | minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. these pathogens must attach to the produce in order to be transmitted. cut surfaces of produce that expose cell walls are particularly vulnerable. little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. using bacterial cellulose-derived plant cell wall models ... | 2015 | 26567310 |
| comparative evaluation of the antimicrobial activity of 19 essential oils. | in our research on natural compounds efficient against human pathogen or opportunist microorganisms contracted by food or water, the antimicrobial activity of 19 essential oils (eos) was investigated against 11 bacterial species (6 gram positive, 5 gram negative) and 7 fungal species (2 dermatophytes, 1 mould, 4 yeasts) using microdilution assays. five essential oils were obtained from tunisian plants (eotun): artemisia herba-alba asso, juniperus phoenicea l., rosmarinus officinalis l., ruta gra ... | 2016 | 26566647 |
| characterization of wastewater effluents releasing from slaughterhouses in and around hyderabad city (india). | the present study was undertaken to determine the pollution load to the environment causing by the wastewater effluents releasing from organized and unorganized slaughterhouses in and around hyderabad city. the wastewater effluents collected from three slaughterhouses, at the sites of releasing out to the surrounding environment,were characterized in terms of physico-chemical and microbiological parameters. the physico-chemical parameters, such as temperature, ph, alkalinity, turbidity, total su ... | 2014 | 26563081 |
| adam17 controls il-6 signaling by cleavage of the murine il-6rα from the cell surface of leukocytes during inflammatory responses. | the cytokine il-6 is part of a regulatory signaling network that controls immune responses. il-6 binds either to the membrane-bound il-6 receptor-α (classic signaling) or to the soluble il-6 receptor-α (trans-signaling) to initiate signal transduction via gp130 activation. because classic and trans-signaling of il-6 fulfill different tasks during immune responses, controlled shedding of the membrane-bound il-6 receptor-α from the surface of immune cells can be considered a central regulator of i ... | 2016 | 26561568 |
| an interactomic approach for identification of putative drug targets in listeria monocytogenes. | a wide variety of human population is infected with listeria monocytogenes, which causes listeriosis, a deadly disease with mortality rate of about 30%. the major hindrance to cure listeriosis is the unavailability of specific or selectable drug targets. at present, antibiotics used to cure the disease are not specific and insufficient to manage the disease efficiently. therefore, in order to search specific drugs, here, we used interactome analysis to search specific drug targets which may prov ... | 2015 | 26561317 |
| antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products. | abstractlactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolat ... | 2015 | 26560661 |
| technological properties and probiotic potential of lactobacillus fermentum strains isolated from west african fermented millet dough. | throughout africa, food fermentations are still driven by indigenous microorganisms which influence the nutritional, organoleptic and safety of the final products. however, for improved safety, consistent quality and beneficial health effects, a trend has emerged which involves the isolation of indigenous strains from traditional fermented products to be used as functional starter cultures. these functional starter cultures possess inherent functional characteristics and can contribute to food q ... | 2015 | 26560346 |
| listeria monocytogenes meningitis in an immunosuppressed patient with autoimmune hepatitis and igg4 subclass deficiency. | a 51-year-old caucasian woman with listeria monocytogenes meningitis was treated and discharged after an uncomplicated course. her medical history included immunosuppressive treatment with prednisolone and azathioprine for autoimmune hepatitis. a diagnostic work-up after the meningitis episode revealed that she had low levels of the igg4 subclass. to our knowledge, this is the first case report describing a possible association between autoimmune hepatitis and the occurrence of listeria monocyto ... | 2015 | 26558118 |
| prevalence of foodborne pathogens in freshwater fish in latvia. | the aim of this study was to detect the prevalence of salmonella spp., listeria monocytogenes, and yersinia enterocolitica in freshwater fish in latvia. in total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from april 2014 to december 2014 in latvia. samples were tested according to international organization for standardization methods. no salmonella spp. were found in fresh fish from lakes or in commercially avail ... | 2015 | 26555535 |
| predominance and distribution of a persistent listeria monocytogenes clone in a commercial fresh mushroom processing environment. | a longitudinal study was conducted to determine the prevalence of listeria spp. in a commercial fresh mushroom slicing and packaging environment. samples were collected at three different sampling periods within a 13-month time interval. of the 255 environmental samples collected, 18.8% tested positive for l. monocytogenes, 4.3% for l. innocua, and 2.0% for l. grayi. l. monocytogenes was most often found on wet floors within the washing and slicing and packaging areas. each of the 171 l. monocyt ... | 2015 | 26555522 |
| disease burden of invasive listeriosis and molecular characterization of clinical isolates in taiwan, 2000-2013. | the information about disease burden and epidemiology of invasive listeriosis in asia is scarce. from 2000 to 2013, a total of 338 patients with invasive listeriosis (bacteremia, meningitis, and peritonitis) were treated at four medical centers in taiwan. the incidence (per 10,000 admissions) of invasive listeriosis increased significantly during the 14-year period among the four centers (0.15 in 2000 and >1.25 during 2010-2012) and at each of the four medical centers. among these patients, 45.9 ... | 2015 | 26555445 |
| listeria monocytogenes associated kerato-conjunctivitis in four horses in norway. | listeria monocytogenes has been reported to cause various infectious diseases in both humans and animals. more rarely, ocular infections have been reported. to our knowledge, only two cases of listeria keratitis have been described in horses. we report kerato-conjunctivitis in four norwegian horses associated with l. monocytogenes. clinically, all cases were presented with recurrent unilateral kerato-conjunctivitis. l. monocytogenes bacteria were isolated from swab samples from all cases, and cy ... | 2015 | 26552393 |
| induced systemic listeriosis in alectoris chukar chicks: clinical, histopathological and microbiological findings. | 1. systemic listeriosis was induced in 14-d-old chukar partridge chicks, alectoris chukar, by intravenous injection of a suspension containing 10(6) cfu/ml of viable listeria monocytogenes organisms to study the course of infection. 2. septicaemic and encephalitic forms of listeriosis were observed in all birds. infection resulted in a fever response 8-h post-inoculation. disease rapidly developed over a 24-h period with decreased activity, lethargy, ruffled feathers, huddling, listlessness, ina ... | 2015 | 26551997 |
| mir-150 regulates differentiation and cytolytic effector function in cd8+ t cells. | micrornas regulate most mammalian genes, and they control numerous aspects of immune system development and function. their precise roles in the cd8+ t cell response, however, remain unclear. in this report, we show that in the absence of the microrna mir-150, cd8+ t cells fail to undergo robust expansion and differentiation into short-lived terminal effector cells in response to primary infection with listeria monocytogenes or vaccinia virus. notably, even after transitioning into the memory po ... | 2015 | 26549197 |
| the opposite effects of dna and protein components of listeria monocytogenes and toxoplasma gondii on immunologic characteristics of dendritic cells. | the innate immune system utilizes pattern recognition receptors (prrs) to recognize microbes. pathogens contain various molecules with diverse effects on immune response. in this study, we evaluated the effect of dna and protein components derived from two intracellular microorganisms including listeria monocytogenes (l. monocytogenes) and toxoplasma gondii (t. gondii) on dendritic cells (dcs) activation and ensuing adaptive immune responses. dna and protein components of l. monocytogenes and t. ... | 2015 | 26546901 |
| up-regulation of cyld enhances listeria monocytogenes induced apoptosis in thp-1 cells. | listeria monocytogenes (lm), a facultative anaerobic gram-positive bacterium, causes listeriosis. immune cell apoptosis is considered to be one pathogenic factor for listeriosis. as a deubiquitinase, cyld is an important regulator both in innate immune response and apoptosis by negatively modulating nf-κb pathway. however the role of cyld in lm induced apoptosis remains unclear. here we found that cyld is significantly up-regulated in macrophages upon its infection. there is a moderate decrease ... | 2016 | 26545866 |
| vital signs: multistate foodborne outbreaks - united states, 2010-2014. | millions of u.s. residents become ill from foodborne pathogens each year. most foodborne outbreaks occur among small groups of persons in a localized area. however, because many foods are distributed widely and rapidly, and because detection methods have improved, outbreaks that occur in multiple states and that even span the entire country are being recognized with increasing frequency. | 2015 | 26540483 |
| foodborne pathogens prevention and sensory attributes enhancement in processed cheese via flavoring with plant extracts. | cheese contaminations with foodborne bacterial pathogens, and their health outbreaks, are serious worldwide problems that could happen from diverse sources during cheese production or storage. plants, and their derivatives, were always regarded as the potential natural and safe antimicrobial alternatives for food preservation and improvement. the extracts from many plants, which are commonly used as spices and flavoring agents, were evaluated as antibacterial agents against serious foodborne pat ... | 2015 | 26540146 |
| survival of unstressed and acid-, cold-, and starvation-stress-adapted listeria monocytogenes in ham extract with hops beta acids and consumer acceptability of hba on ready-to-eat ham. | the efficacy of hops beta acids (hba) against unstressed and stress-adapted listeria monocytogenes in ham extract and the consumers' acceptability of hba on ready-to-eat (rte) hams were investigated. unstressed or acid-, cold-, or starvation-stress-adapted l. monocytogenes was inoculated (1.3-1.5 log cfu/ml) into 10% ham extract, without (control) or with hba (4.44 or 10.0 µg/ml). survival/growth of the pathogen during storage (7.2 °c, 26 days) was monitored periodically. sensory evaluation (30 ... | 2015 | 26539527 |
| impact of moderate heat, carvacrol, and thymol treatments on the viability, injury, and stress response of listeria monocytogenes. | the microbial safety and stability of minimally processed foods are based on the application of combined preservative factors. since microorganisms are able to develop adaptive networks to survive under conditions of stress, food safety may be affected, and therefore understanding of stress adaptive mechanisms plays a key role in designing safe food processing conditions. in the present study, the viability and the sublethal injury of listeria monocytogenes exposed to moderate heat (55 °c) and/o ... | 2015 | 26539510 |
| twenty years of listeria in brazil: occurrence of listeria species and listeria monocytogenes serovars in food samples in brazil between 1990 and 2012. | listeria spp. isolated from different food products and collected from 12 brazilian states were sent to the laboratory of bacterial zoonoses (oswaldo cruz institute, brazil) for identification. the aims of this study were to characterize these isolates, from 1990 to 2012, by using biochemical, morphological, and serotyping tests, and to analyze the distribution of l. monocytogenes serotypes on different food products and geographical locations. serotyping was performed using polyclonal somatic a ... | 2015 | 26539507 |
| uv-heat treatments for the control of foodborne microbial pathogens in chicken broth. | this investigation established the process criteria for using uv-c light and mild heat (uv-h treatment) to inactivate 5-log10 cycles (performance criterion) of common foodborne pathogen populations, escherichia coli, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus, when inoculated in chicken broth. to define the target microorganism and the proper uv-h treatment conditions (including uv dose, treatment time, and temperature) that would achieve the stated performance cri ... | 2015 | 26539493 |
| comparison of primary models to predict microbial growth by the plate count and absorbance methods. | the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ... | 2015 | 26539483 |