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fate of bacillus cereus and naturally occurring microbiota on milled rice as affected by temperature and relative humidity.we studied the survival and growth patterns of bacillus cereus, mesophilic aerobic bacteria (mab), and molds and yeasts (my) on rough and milled brown and white korean rice stored at 12 and 21 °c and 43, 68, and 85% relative humidity (rh) for up to 24 wk. the initial populations of mab present on rough rice, brown rice, and white rice were 7.7, 5.7, and 3.3 log cfu/g, respectively, and remained constant or decreased (p ≤ 0.05) by 0.7-1.8 log cfu/g during storage. the initial populations of b. ce ...201424290634
contrasting evolutionary patterns of spore coat proteins in two bacillus species groups are linked to a difference in cellular structure.the bacillus subtilis-group and the bacillus cereus-group are two well-studied groups of species in the genus bacillus. bacteria in this genus can produce a highly resistant cell type, the spore, which is encased in a complex protective protein shell called the coat. spores in the b. cereus-group contain an additional outer layer, the exosporium, which encircles the coat. the coat in b. subtilis spores possesses inner and outer layers. the aim of this study is to investigate whether differences ...201324283940
enhanced biosynthesis of poly(3-hydroxybutyrate) from potato starch by bacillus cereus strain 64-ins in a laboratory-scale fermenter.to decrease the polyhydroxyalkanoate (pha) production cost by supplying renewable carbon sources has been an important aspect in terms of commercializing this biodegradable polymer. the production of biodegradable poly(3-hydroxyalkanoates) (pha) from raw potato starch by the bacillus cereus 64-ins strain isolated from domestic sludge has been studied in a lab-scale fermenter. the bacterium was screened for the degradation of raw potato starch by a starch hydrolysis method and for pha production ...201424279753
isolation, screening and production studies of uricase producing bacteria from poultry sources.uricase (urate oxidase ec 1.7.3.3) is a therapeutic enzyme that is widely used to catalyze the enzymatic oxidation of uric acid in the treatment of hyperuricemia and gout diseases. in this study, three bacterial species capable of producing extracellular uricase were isolated from a poultry source and screened based on the size of the clear zone using a uric acid agar plate. the bacterial species capable of producing uricase with the highest uricolytic activity was identified as bacillus cereus ...201424279683
isolation and identification of a new intracellular antimicrobial peptide produced by paenibacillus alvei an5.a wild-type, gram-positive, rod-shaped, endospore-forming and motile bacteria has been isolated from palm oil mill sludge in malaysia. molecular identification using 16s rrna gene sequence analysis indicated that the bacteria belonged to genus paenibacillus. with 97 % similarity to p. alvei (aug6), the isolate was designated as p. alvei an5. an antimicrobial compound was extracted from p. alvei an5-pelleted cells using 95 % methanol and was then lyophilized. precipitates were re-suspended in pho ...201424272828
development of a novel selective and differential medium for the isolation of listeria monocytogenes.a new medium (lecithin and levofloxacin [ll] medium) is described for the isolation of listeria monocytogenes from food samples. ll medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase c (pi-plc) and phosphatidylcholine-specific phospholipase c (pc-plc) produced by l. monocytogenes. levofloxacin is incorporated to inhibit the growth of microorganisms other than l. monocytogenes, especially bacillus cereus, shown to possess pi-plc and pc-plc acti ...201424271177
novel highly thermostable endolysin from thermus scotoductus mat2119 bacteriophage ph2119 with amino acid sequence similarity to eukaryotic peptidoglycan recognition proteins.in this study, we present the discovery and characterization of a highly thermostable endolysin from bacteriophage ph2119 infecting thermus strain mat2119 isolated from geothermal areas in iceland. nucleotide sequence analysis of the 16s rrna gene affiliated the strain with the species thermus scotoductus. bioinformatics analysis has allowed identification in the genome of phage 2119 of an open reading frame (468 bp in length) coding for a 155-amino-acid basic protein with an mr of 17,555. ph211 ...201424271162
characterization and comparative genomic analysis of bacteriophages infecting members of the bacillus cereus group.the bacillus cereus group phages infecting b. cereus, b. anthracis, and b. thuringiensis (bt) have been studied at the molecular level and, recently, at the genomic level to control the pathogens b. cereus and b. anthracis and to prevent phage contamination of the natural insect pesticide bt. a comparative phylogenetic analysis has revealed three different major phage groups with different morphologies (myoviridae for group i, siphoviridae for group ii, and tectiviridae for group iii), genome si ...201424264384
an insect gut environment reveals the induction of a new sugar-phosphate sensor system in bacillus cereus.bacteria survive under various conditions by sensing stimuli triggering specific adaptive physiological responses, which are often based on membrane-integrated sensors connected to a cytoplasmic regulator. recent studies reveal that mucus glycans may act as signal molecules for two-component systems involved in intestinal colonization. bacillus cereus, a human and insect opportunistic pathogen was used to identify bacterial factors expressed in an insect gut infection model. the screen revealed ...201524256737
effects of a skin neuropeptide (substance p) on cutaneous microflora.skin is the largest human neuroendocrine organ and hosts the second most numerous microbial population but the interaction of skin neuropeptides with the microflora has never been investigated. we studied the effect of substance p (sp), a peptide released by nerve endings in the skin on bacterial virulence.201324250813
effect of apigeninidin on the growth of selected bacteria.apigeninidin, a 3-deoxyanthocyanidin present in sorghum, was chemically synthesized and effectively purified by extracting impurities from aqueous 2 n hc1 into ethyl acetate. apigeninidin (50μmol) applied to filter paper disks inhibited the growth on agar plates of selected gram-positive bacteria (bacillus cereus, staphylococcus aureus, staphylococcus epidermidis, andstreptococcus faecalis) and to a lesser extent gram-negative bacteria (escherichia coli, serratia marcens, andshigella flexneri). ...199324249081
a new bioassay system for screening high berberine-producing cell colonies of thalictrum minus.a new method of estimating the amount of berberine released from minute cell colonies of thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. in this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against bacillus cereus mt2026. screening of 1000 ...198724248650
nmr assignments for the cis and trans forms of the hemolysin ii c-terminal domain.pathogenic bacteria secrete pore-forming toxins (pfts) to selectively defend against immune cells and to break through cellular barriers in the host. understanding how pfts attack cell membranes is not only essential for therapeutic intervention but for designing agents to deliver drugs to specific human cell subtypes, for example in anti-cancer or anti-viral therapies. many toxins contain accessory domains that help recognize specific molecular epitopes on the membranes of target cells, includi ...201424234348
improving glyphosate oxidation activity of glycine oxidase from bacillus cereus by directed evolution.glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (go) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the c-n bond in glyphosate. here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a glyphosate-degrading marine strain of bacillus cereus (bcego), we used a bacteriophage ...201324223901
antimicrobial activities of leaf extracts of guava (psidium guajava l.) on two gram-negative and gram-positive bacteria.aim. to determine the antimicrobial potential of guava (psidium guajava) leaf extracts against two gram-negative bacteria (escherichia coli and salmonella enteritidis) and two gram-positive bacteria (staphylococcus aureus and bacillus cereus) which are some of foodborne and spoilage bacteria. the guava leaves were extracted in four different solvents of increasing polarities (hexane, methanol, ethanol, and water). the efficacy of these extracts was tested against those bacteria through a well-di ...201324223039
equilibrium selectivity alone does not create k+-selective ion conduction in k+ channels.potassium (k(+)) channels are selective for k(+) over na(+) ions during their transport across membranes. we and others have previously shown that tetrameric k(+) channels are primarily occupied by k(+) ions in their selectivity filters under physiological conditions, demonstrating the channel's intrinsic equilibrium preference for k(+) ions. based on this observation, we hypothesize that the preference for k(+) ions over na(+) ions in the filter determines its selectivity during ion conduction. ...201324217508
bacillus cereus enterotoxins act as major virulence factors and exhibit distinct cytotoxicity to different human cell lines.a comparative analysis on the relevance of the bacillus cereus enterotoxins nhe (nonhemolytic enterotoxin), hbl (haemolysin bl) and cytk (cytotoxin k) was accomplished, concerning their toxic activity towards different target cell lines. overall, among the components secreted by the reference strains for nhe and hbl, the enterotoxin complexes accounted for over 90% of the total toxicity. vero and primary endothelial cells (huvec) were highly susceptible to nhe, whereas hep-g2, vero and a549 reac ...201424211313
prevalence, pfge typing, and antibiotic resistance of bacillus cereus group isolated from food in morocco.this article reports the prevalence and antibiotic resistance of the bacillus cereus group isolated from different foods (milk and dairy products, spices, and rice salad) in morocco. in total, 402 different food samples collected from 2008 to 2010 were analyzed by microbiological methods to isolate b. cereus. the strains were subjected to a polymerase chain reaction test in order to verify whether they belonged to the b. cereus group. sixty-four of all isolates (15.9%) were found to be positive. ...201424206436
root exudate-induced alterations in bacillus cereus cell wall contribute to root colonization and plant growth promotion.the outcome of an interaction between plant growth promoting rhizobacteria and plants may depend on the chemical composition of root exudates (res). we report the colonization of tobacco, and not groundnut, roots by a non-rhizospheric bacillus cereus (mtcc 430). there was a differential alteration in the cell wall components of b. cereus in response to the res from tobacco and groundnut. attenuated total reflectance infrared spectroscopy revealed a split in amide i region of b. cereus cells expo ...201324205213
the bacillus cereus hbl and nhe tripartite enterotoxin components assemble sequentially on the surface of target cells and are not interchangeable.bacillus cereus is a spore-forming, gram-positive bacterium commonly associated with outbreaks of food poisoning. it is also known as an opportunistic pathogen causing clinical infections such as bacteremia, meningitis, pneumonia, and gas gangrene-like cutaneous infections, mostly in immunocompromised patients. b. cereus secretes a plethora of toxins of which four are associated with the symptoms of food poisoning. two of these, the non-hemolytic enterotoxin nhe and the hemolysin bl (hbl) toxin, ...201324204713
effect of different in vitro culture extracts of black pepper (piper nigrum l.) on toxic metabolites-producing strains.in the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (escherichia coli, pseudomonas aeroginosa, salmonella typhi, bacillus subtilis, bacillus cereus, staphylococcus aureus, and candida albicans). these in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion me ...201624193053
identification and characterization of a novel marine bacillus cereus for mosquito control.entomopathogenic bacteria to control mosquitoes are a promising environmentally friendly alternative to synthetic pesticides. in the present study, a novel mosquitocidal bacterium was isolated from marine soil collected from east coastal areas at pondicherry (india). 16s rrna gene sequence alignment depicted that this isolate belonged to bacillus cereus vcrc-b520 (ncbi: kc-119192). biochemical studies on bacterial growth, biomass, and toxin production have revealed that this strain could possibl ...201424192866
interactions of bacteria and microflagellates in sequencing batch reactors exhibiting enhanced mineralization of toxic organic chemicals.community level interactions were studied in non-axenic sequencing batch reactors (sbrs) being used to treat 2,4-dinitrophenol (dnp). increasing the influent dnp concentrations from 1 to 10 µg ml(-1) eliminated large predatory organisms such as rotifers and ciliated protozoa from the sbrs. under steady-state conditions at a dnp concentration of 10 µg ml(-1), supplemental additions of glucose enhanced dnp degradation and led to the establishment of a microbial community consisting of five species ...199224192859
phytochemical analysis of andrographis paniculata and orthosiphon stamineus leaf extracts for their antibacterial and antioxidant potential.leaves of andrographis paniculata and orthosiphon stamineus were extracted with water, ethanol, methanol and chloroform to assess their potential as antibacterial and antioxidant agents. high performance liquid chromatography analysis showed that the methanolic extracts of a. paniculata and o. stamineus leaves gave the highest amounts of andrographolide and rosmarinic acid, respectively. these leaf extracts exhibited antimicrobial and antioxidant activities and, at the highest concentration test ...201324189677
ohrra functions as a redox-responsive system controlling toxinogenesis in bacillus cereus.bacillus cereus ohrr is a member of the subgroup of the marr (multiple antibiotic resistance) family of transcriptional regulators that use a cysteine-based redox sensing mechanism. ohra is a thiol-dependent, peroxidase-like protein. the dual ohrra system triggers b. cereus adaptation in response to redox changes, such as those encountered in the environment of the gastrointestinal tract. here, we investigated the role of ohrra in toxinogenesis. comparative shotgun analysis of exoproteomes from ...201324184231
development of new active packaging film made from a soluble soybean polysaccharide incorporated zataria multiflora boiss and mentha pulegium essential oils.an active edible film from soluble soybean polysaccharide (ssps) incorporated with different concentrations of zataria multiflora boiss (zeo) and mentha pulegium (meo) essential oils was developed, and the film's optical, wettability, thermal, total phenol and antioxidant characteristics were investigated, along with their antimicrobial effectiveness against staphylococcus aureus, bacillus cereus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium. the film's colour beca ...201424176389
inference of the properties of the recombination process from whole bacterial genomes.patterns of linkage disequilibrium, homoplasy, and incompatibility are difficult to interpret because they depend on several factors, including the recombination process and the population structure. here we introduce a novel model-based framework to infer recombination properties from such summary statistics in bacterial genomes. the underlying model is sequentially markovian so that data can be simulated very efficiently, and we use approximate bayesian computation techniques to infer paramete ...201324172133
the designer proline-rich antibacterial peptide a3-apo prevents bacillus anthracis mortality by deactivating bacterial toxins.proline-rich antibacterial peptides protect experimental animals from bacterial challenge even if they are unable to kill the microorganisms in vitro. their major in vivo modes of action are inhibition of bacterial protein folding and immunostimulation. here we investigated whether the proline-rich antibacterial peptide dimer a3-apo was able to inhibit bacillus cereus enterotoxin production in vitro and restrict the proliferation of lethal toxin-induced bacillus anthracis replication in mouse ma ...201424164262
competition as a source of errors in rapd analysis.we have used artificial 1∶1 dna mixtures of all pairwise combinations of four doubled haploid brassica napus lines to test the ability of rapds to function as reliable dominant genetic markers. in situations where a specific rapd band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 dna mixture. in 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the rapd reaction. thus, rapd mark ...199624162529
retained subretinal date palm tree thorn in a child.a 3-year-old boy presented with severe eye pain and nausea after colliding with a date palm tree branch. examination under anesthesia revealed a self-sealed corneal laceration and traumatic cataract in his left eye. cultures were taken and the patient received prophylactic subconjunctival, intravitreal, and systemic antibiotics because of the high risk of endophthalmitis. a thorn from the same tree grew bacillus cereus. examination after 2 weeks of the injury revealed a subretinal foreign body ( ...201324160979
fusarial wilt control and growth promotion of pigeon pea through bioactive metabolites produced by two plant growth promoting rhizobacteria.the bioactive metabolites produced by two plant growth promoting rhizobacteria strains, a pseudomonas aeruginosa strain rrlj 04 and a bacillus cereus strain bs 03, which showed growth promotion and disease control in pigeon pea against fusarium udum, were isolated and screened for their efficacy to control fusarial wilt of pigeon pea under gnotobiotic and nursery condition. bioactive metabolites viz., bm 1 and bm 2 from rrlj 04 and bm 3 from bs 03 also showed in vitro antibiosis against f. udum. ...201424154979
novel antibacterial activity of lactococcus lactis subspecies lactis z11 isolated from zabady.the purpose of this study was to select and characterize a probiotic bacterium with distinctive antimicrobial activities. in this respect, lactococcus lactis subspecies lactis z11 (l. lactis z11) isolated from zabady (arabian yoghurt) inhibited other strains of lactic acid bacteria and some food-born pathogens including listeria monocytogenes, bacillus cereus and staphylococcus aureus. the inhibitory activity of cell free supernatant (cfs) of l. lactis z11 isolated from zabady was lost by proteo ...201324151453
antibacterial activity of phyllantus emblica, coriandrum sativum, culinaris medic, lawsonia alba and cucumis sativus.present study deals with the demonstration of the antibacterial activity of very common medicinal plants of pakistani origin i.e., phyllantus emblica, coriandrum sativum, culinaris medic, lawsonia alba and cucumis sativus. the extracts were prepared in crude form by the use of hydro-alcoholic solution and were screened for antibacterial activity against various bacterial species by disk diffusion method. assay was performed using clinical isolates of b. cereus, s. aureus, p. aeruginosa and e. co ...201324147363
pahangensin a and b, two new antibacterial diterpenes from the rhizomes of alpinia pahangensis ridley.the rhizomes of alpinia pahangensis ridley yielded a new bis-labdanic diterpene for which the name pahangensin a (1) was proposed along with a new labdane diterpene, pahangensin b (2). their structures were elucidated by spectroscopic methods including, 1d and 2d nmr techniques and lcms-it-tof analysis. pahangensin a (1) was found to be an antibacterial agent against staphylococcus aureus, bacillus cereus and bacillus subtilis with mic values less than 100 μg/ml, respectively. pahangensin b (2) ...201324144849
physical chemical and biological characterization of a new bacteriocin produced by bacillus cereus ns02.to screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical, chemical and biological aspects for the application in biopreservation.201324144023
characterization of a cold-active β-glucosidase from paenibacillus xylanilyticus kj-03 capable of hydrolyzing isoflavones daidzin and genistin.paenibacillus xylanilyticus kj-03 isolated from konjac field, showed β-glucosidase activity on tryptic soy agar plate supplemented with 0.1 % esculin and 0.25 % ferric ammonium citrate. a genome library was constructed to obtain the β-glucosidase gene and a recombinant clone, pglc2-3 was selected. the 2,247 bp gene encoding kj-03 β-glucosidase consisted of 749 amino acids. the deduced amino acids of bgla were 61 % homologous with that of the β-glucosidase from bacillus cereus ah1272, which belon ...201324141566
potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma.atmospheric pressure non-thermal plasma (apntp) has been gaining increasing interest as a new alternative antibacterial approach. although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. mechanistic elucidation of the antimicrobial activity will facilitate development and rational optimisation of this approach for potential medical applications. in this study, the antibacterial efficacy of an in-house-built apntp jet was evaluated a ...201424139886
the relative importance of competition and predation in environment characterized by resource pulses--an experimental test with a microbial community.resource availability and predation are believed to affect community dynamics and composition. although the effects of resource availability and predation on prey communities are usually studied in isolation, these factors can also have interactive effects, especially since the outcome of competition under shared predation is expected to depend on resource availability. however, there are few experimental studies that test the interactive roles of resources and predation on dynamics of more comp ...201324139511
mercuric reductase activity of multiple heavy metal-resistant lysinibacillus sphaericus g1.a culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate cd, co, zn, cr, and hg up to 190, 525, 350, 935, and 370 μm, respectively. the isolate was identified as lysinibacillus sphaericus by 16s rrna gene sequencing. it bioaccumulated cd, co, and zn, and reductively detoxified cr and hg. chromate reductase and mercuric reductase (mera) activities in the cell extract were 2.4 and 0.13 units mg(-1) protein, respectively. the study also describes designing of ...201524132860
toxin producing bacillus cereus persist in ready-to-reheat spaghetti bolognese mainly in vegetative state.the potential of bacillus cereus to cause a diarrheal toxico-infection is related to its ability to perform de novo enterotoxin production in the small intestine. a prerequisite for this is presence of sufficient numbers of b. cereus that have survived gastro-intestinal passage. it is known that the percentage of survival is much smaller for vegetative cells in comparison to spores and it is therefore important to know the state in which b. cereus is ingested. the results of the current study pe ...201324129156
bacillus cereus var. toyoi modulates the immune reaction and reduces the occurrence of diarrhea in piglets challenged with salmonella typhimurium dt104.a feeding trial with sows and their piglets was performed with the probiotic feed additive bacillus cereus var. toyoi in two consecutive experimental periods. sows (n = 8) were allocated into treatment (bc) and control (co) groups. sows of bc group (n = 4) were fed 3.14 × 10(5) cfu/g bacillus cereus var. toyoi with the diet from d 87 of pregnancy on. their piglets received bacillus cereus var. toyoi supplemented feed (8.7 × 10(5) cfu/g) starting on d 14 of life and further on after weaning (6.5 ...201324126275
volatile analysis and antimicrobial screening of the parasitic plant cuscuta reflexa roxb. from nepal.the essential oil from the parasitic vine cuscuta reflexa roxb., collected from kirtipur, kathmandu, nepal, was obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry. from a total of 62 peaks, 61 compounds were identified in the oil, accounting for 99.6% of the oil. the majority of the essential oil was dominated by the relatively rare component cis-3-butyl-4-vinylcyclopentane (26.4%). the oil also contained substantial amounts of limonene (5.1%) and (e)-nerolidol (9 ...201424116676
comparison of antibacterial activity of ag nanoparticles synthesized from leaf extract of parthenium hystrophorus l in aqueous media and gentamicin sulphate: in-vitro.monodisperse silver (ag) nanoparticles were synthesized by using parthenium hystrophorus l leaf extract in aqueous media. the synthesized nanoparticles were characterized by using uv-vis spectrophotometer, x-ray diffracto-meter (xrd), transmission electron microscope (tem), and dynamics light scattering (dls). size-dependent antibacterial activities of ag nanoparticles were tested against gram negative pseudomonas aeruginosa and gram positive staphylococcus aureus. ag nanoparticles having 20 ± 2 ...201524111829
bacillus cereus cell response upon exposure to acid environment: toward the identification of potential biomarkers.microorganisms are able to adapt to different environments and evolve rapidly, allowing them to cope with their new environments. such adaptive response and associated protections toward other lethal stresses, is a crucial survival strategy for a wide spectrum of microorganisms, including food spoilage bacteria, pathogens, and organisms used in functional food applications. the growing demand for minimal processed food yields to an increasing use of combination of hurdles or mild preservation fa ...201324106490
rna-seq analysis of antibiotic-producing bacillus subtilis sc-8 in response to signal peptide papr of bacillus cereus.bacillus subtilis sc-8 produces an antibiotic that has narrow antagonistic activity against bacteria in the bacillus cereus group. in b. cereus group bacteria, peptide-activating plcr (papr) plays a significant role in regulating the transcription of virulence factors. when b. subtilis sc-8 and b. cereus are co-cultured, papr is assumed to stimulate antibiotic production by b. subtilis sc-8. to better understand the effect of papr on this interspecies interaction, the global transcriptome profil ...201424104687
antimicrobial constituents of the leaves of mikania micrantha h. b. k.to isolate plant-derived compounds with antimicrobial activity from the leaves of mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (exserohilum turcicum, colletotrichum lagenarium, pseudoperonispora cubensis, botrytis cirerea, rhizoctonia solani, phytophthora parasitica, fusarium solani, and pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: ralstonia dolaanacearum, xan ...201324098556
cyclo(l-pro-d-arg): a new antibacterial and antitumour diketopiperazine from bacillus cereus associated with a rhabditid entomopathogenic.in continuation of our search for new antimicrobial secondary metabolites from bacillus cereus associated with rhabditid entomopathogenic nematode, a new microbial diketopiperazine, cyclo(l-pro-d-arg), was isolated from the ethyl acetate extract of fermented modified nutrient broth. the chemical structures of the isolated compounds were identified based on their 1d, 2d nmr and high-resolution electrospray ionisation-mass spectroscopy data. antibacterial activity of the compound was determined by ...201424097052
functional role of the mrpa- and mrpd-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex i.nadh:quinone oxidoreductase or complex i is a large membrane bound enzyme complex that has evolved from the combination of smaller functional building blocks. intermediate size enzyme complexes exist in nature that comprise some, but not all of the protein subunits in full size 14-subunit complex i. the membrane spanning complex i subunits nuol, nuom and nuon are homologous to each other and to two proteins from one particular class of na(+)/h(+) antiporters, denoted mrpa and mrpd. in complex i, ...201424095649
bacillus cereus sensu lato genomes: basis for identifying anthrax disease strain sources. 201324092785
whole-genome sequences of 94 environmental isolates of bacillus cereus sensu lato.bacillus cereus sensu lato is a species complex that includes the anthrax pathogen bacillus anthracis and other bacterial species of medical, industrial, and ecological importance. their phenotypes of interest are typically linked to large plasmids that are closely related to the anthrax plasmids pxo1 and pxo2. here, we present the draft genome sequences of 94 isolates of b. cereus sensu lato, which were chosen for their plasmid content and environmental origins.201324092776
nisin-activated hydrophobic and hydrophilic surfaces: assessment of peptide adsorption and antibacterial activity against some food pathogens.an effective antimicrobial packaging or food contact surface should be able to kill or inhibit micro-organisms that cause food-borne illnesses. setting up such systems, by nisin adsorption on hydrophilic and hydrophobic surfaces, is still a matter of debate. for this purpose, nisin was adsorbed on two types of low-density polyethylene: the hydrophobic native film and the hydrophilic acrylic acid-treated surface. the antibacterial activity was compared for those two films and it was highly depend ...201324092009
in vitro cytotoxic, antioxidant, and antimicrobial activities of mesua beccariana (baill.) kosterm., mesua ferrea linn., and mesua congestiflora extracts.the in vitro cytotoxicity tests on the extracts of mesua beccariana, m. ferrea, and m. congestiflora against raji, snu-1, hela, ls-174t, nci-h23, sk-mel-28, hep-g2, imr-32, and k562 were achieved using mtt assay. the methanol extracts of mesua beccariana showed its potency towards the proliferation of b-lymphoma cell (raji). in addition, only the nonpolar to semipolar extracts (hexane to ethyl acetate) of the three mesua species indicated cytotoxic effects on the tested panel of human cancer cel ...201324089682
biodegradation of decabromodiphenyl ether (bde-209) by a metal resistant strain, bacillus cereus jp12.a metal resistant bacterial strain, bacillus cereus jp12, could use decabromodiphenyl ether (bde-209) as the sole carbon and energy source for growth in mineral salt medium. under the conditions of ph 6.0, 30°c, 150 rpm and an inoculum of od600=0.6, more than 88% of the initial bde-209 (1mg/l) was degraded after 12 days. the addition of appropriate surfactants and additional carbon sources could enhance the biodegradation efficiency of bde-209. the presence of cu(2+) (≤ 8 mg/l) and zn(2+) (≤ 15 ...201324084199
diversity of endophytic bacteria in medicinally important nepenthes species.nepenthes species are used in traditional medicines to treat various health ailments. however, we do not know which types of endophytic bacteria (eb) are associated with nepenthes spp.201324082746
chemical composition and biological activity of conyza bonariensis essential oil collected in mérida, venezuela.the essential oil from aerial parts of conyza bonariensis (l) cronquist collected in mérida was obtained by hydrodistillation and analysed by gc/ms. the major components were trans-beta-farnesene (37.8%), trans-ocimene (20.7%) and beta-sesquiphellandrene (9.8%). cytotoxicity assay was also performed with the essential oil against hela (cervix carcinoma), a-459 (lung carcinoma) and mcf-7 (breast adenocarcinoma) human cell lines and against normal vero cells (african green monkey kidney) with ic50 ...201324079198
chemical composition and bioactivity of essential oils of hypericum helianthemoides, hypericum perforatum and hypericum scabrum.a number hypericum species are well known for their therapeutic efficacy and use in traditional medicine. the various species of hypericum have been traditionally used for the treatment of wounds, eczema, burns, trauma, rheumatism, neuralgia, gastroenteritis, ulcers, hysteria, bedwetting and depression.201424073965
use of silkworms to evaluate the pathogenicity of bacteria attached to cedar pollen.injection of a japanese cedar pollen suspension into silkworm hemolymph kills the silkworms. a certain species of bacteria proliferated in the hemolymph of the dead silkworms. a 16s rdna analysis demonstrated that the proliferating bacteria were bacillus cereus, bacillus thuringiensis, bacillus weihenstephanensis, and bacillus amyloliquefaciens. among them, b. cereus, b. thuringiensis, and b. weihenstephanensis exhibited hemolysis against sheep red blood cells and were lethal to mice. a culture ...201324071577
viability of calcifying bacterial formulations in fly ash for applications in building materials.evidence of bacterial involvement in precipitation of calcium carbonates has brought a revolution in the field of applied microbiology, geotechnical sciences, environmental and civil engineering with its marked success in restoration of various building materials. for applications of these calcite binder-producing bacterial cultures, different expensive carrier materials have been used but their high costs have come in the way of their successful commercialization. in the present study, we have ...201324065359
antimicrobial property of extracts of indian lichen against human pathogenic bacteria.context. usnea ghattensis g. awasthi (usneaceae) endemic fruticose lichen found growing luxuriantly in northern western ghats of india, it also contains usnic acid as a major chemical and tested against some human pathogenic bacteria. objective. to explore antimicrobial properties of usnea ghattensis against some human pathogenic bacteria. materials and methods. the lichen was extracted in acetone, methanol, and ethanol. in vitro antimicrobial activity was tested initially by kirby-bauer techniq ...201324062769
pcr detection of cytk gene in bacillus cereus group strains isolated from food samples.a method for detection of the cytotoxin k cytk structural gene and its active promoter preceded by the plcr-binding box, controlling the expression level of this enterotoxin, was developed. the method was applied for the purpose of the analysis of 47 bacterial strains belonging to the bacillus cereus group isolated from different food products. it was found that the majority of the analyzed strains carried the fully functional cytk gene with its plcr regulated promoter. the cytk gene was not det ...201324060693
solution structures of the bacillus cereus metallo-β-lactamase bcii and its complex with the broad spectrum inhibitor r-thiomandelic acid.metallo-β-lactamases, enzymes which inactivate β-lactam antibiotics, are of increasing biological and clinical significance as a source of antibiotic resistance in pathogenic bacteria. in the present study we describe the high-resolution solution nmr structures of the bacillus cereus metallo-β-lactamase bcii and of its complex with r-thiomandelic acid, a broad-spectrum inhibitor of metallo-β-lactamases. this is the first reported solution structure of any metallo-β-lactamase. there are differenc ...201324059435
new derivatives of salicylamides: preparation and antimicrobial activity against various bacterial species.three series of salicylanilides, esters of n-phenylsalicylamides and 2-hydroxy-n-[1-(2-hydroxyphenylamino)-1-oxoalkan-2-yl]benzamides, in total thirty target compounds were synthesized and characterized. the compounds were evaluated against seven bacterial and three mycobacterial strains. the antimicrobial activities of some compounds were comparable or higher than the standards ampicillin, ciprofloxacin or isoniazid. derivatives 3f demonstrated high biological activity against staphylococcus au ...201324045008
synthesis and antimicrobial activity of polyhalobenzonitrile quinazolin-4(3h)-one derivatives.a novel series of polyhalobenzonitrile quinazolin-4(3h)-one derivatives were synthesized and characterized by nmr, ir, ms, and hrms spectra. all of the newly prepared compounds were screened for antimicrobial activities against four strains of bacteria (gram-positive bacterial: staphylococcus aureus and bacillus cereus; gram-negative bacterial: escherichia coli and pseudomonas aeruginosa) and one strain of fungi (candida albicans). among the synthesized compounds, 5-(dimethylamino)-8-(2,4,5-tric ...201324044873
computational analysis of trappc9: candidate gene for autosomal recessive non-syndromic mental retardation.mental retardation (mr)/ intellectual disability (id) is a neuro-developmental disorder characterized by a low intellectual quotient (iq) and deficits in adaptive behavior related to everyday life tasks such as delayed language acquisition, social skills or self-help skills with onset before age 18. to date, a few genes (prss12, crbn, cc2d1a, grik2, tusc3, trappc9, tecr, st3gal3, med23, man1b1, nsun1) for autosomal-recessive forms of non syndromic mr (ns-armr) have been identified and establishe ...201424040793
combination of extracts from aristolochia cymbifera with streptomycin as a potential antibacterial drug.the appearance of new antibiotic-resistant bacteria is a societal problem that requires the development of new alternative treatments. therefore, this work evaluated the antibacterial activity of ethanolic (ehi), dichloromethanic (edi) and hexanic (ehe) extracts from aristolochia cymbifera stems and the combination of these extracts with an antimicrobial drug to develop a new antibacterial therapy. the edi, ehe and ehi extracts were obtained by maceration using three different solvents. the mini ...201324040585
structure of the nhea component of the nhe toxin from bacillus cereus: implications for function.the structure of nhea, a component of the bacillus cereus nhe tripartite toxin, has been solved at 2.05 å resolution using selenomethionine multiple-wavelength anomalous dispersion (mad). the structure shows it to have a fold that is similar to the bacillus cereus hbl-b and e. coli clya toxins, and it is therefore a member of the clya superfamily of α-helical pore forming toxins (α-pfts), although its head domain is significantly enlarged compared with those of clya or hbl-b. the hydrophobic β-h ...201324040335
biosorption of as (iii) by non-living biomass of an arsenic-hypertolerant bacillus cereus strain sz2 isolated from a gold mining environment: equilibrium and kinetic study.the ability of non-living biomass of an arsenic-hypertolerant bacillus cereus strain sz2 isolated from a gold mining environment to adsorb as (iii) from aqueous solution in batch experiments was investigated as a function of contact time, initial as (iii) concentration, ph, temperature and biomass dosage. langmuir model fitted the equilibrium data better in comparison to freundlich isotherm. the maximum biosorption capacity of the sorbent, as obtained from the langmuir isotherm, was 153.41 mg/g. ...201324037600
growth of enterotoxin producing bacillus cereus in meat substrate at 10ºc and 30ºc.the behaviour of enterotoxin-producing bacillus cereus in meat was investigated by inoculating spore suspensions of five cultures into meat substrate (ph 5.8) and incubating at 10ºc and 30ºc. the bacterial populations were evaluated after different times by plate counts in nutrient agar. all the cultures presented growth at 30ºc with the generation time varying from 28.8 to 36.0 minutes. three cultures also presented growth at 10ºc with generation times between 10.16 and 28.38 h. considering the ...201224031969
identification and characterization of the endophytic plant growth prompter bacillus cereus strain mq23 isolated from sophora alopecuroides root nodules.endophytes mq23 and mq23r isolated from sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. results showed that mq23 and mq23r are potential n2-fixing endophytes and belong to the same species as bacillus cereus. mq23 was shown to be able to produce siderophores, iaa, and demonstrate certain antifungal activity to plant pathogenic fungi. co-inoculation with mq23+mq23ii showed a more significant effe ...201124031669
assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of bacillus cereus from a dairy plant.the interaction between the surface of stainless steel and bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of b. cereus and stainless steel in the presence or absence of b. cereus adherence. the microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by b. cereus were evaluated with the help of atomic force microscopy and perfilometry. the strain of ...201024031578
feather degradation by strains of bacillus isolated from decomposing feathers.feather waste is generated in large amounts as a by-product of commercial poultry processing. this residue is almost pure keratin, which is not easily degradable by common proteolytic enzymes. eight strains of bacillus, isolated from decomposing feathers were tested for the hydrolysis of feather wastes in the laboratory. among these strains, bacillus cereus kb043 was the best feather degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. it ...201024031481
antimicrobial activity of enterococcus faecium fair-e 198 against gram-positive pathogens.this study investigated the antimicrobial activity of enterococcus faecium fair-e 198 against bacillus cereus, listeria monocytogenes and staphylococcus aureus. using the critical-dilution method, the bacteriocin produced by e. faecium fair-e 198 inhibited all l. monocytogenes strains evaluated (1,600 to 19,200 au ml(-1)). however, none of the b. cereus and s. aureus strains investigated were inhibited. the maximum activity of this bacteriocin (800 au ml(-1)) was observed in mrs broth, while the ...201024031466
biodegradability of commercial and weathered diesel oils.this work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 -dichlorophenol indophenol (dcpip) and soil respirometric experiments using biometer flasks. in the former we tested the bacterial cultures staphylococcus hominis, kocuria palustris, ...200824031193
skin preparation before hip replacement in emergency setting versus elective scheduled arthroplasty: bacteriological comparative analysis.hip arthroplasty needs to be performed in an emergency setting after intracapsular femur neck fracture, whereas pain makes preoperative skin preparation of the limb difficult and it may therefore be incomplete. to date no study has analyzed the patient's skin bacteriological status in these surgical conditions.201324029588
a quantitative microbiological exposure assessment model for bacillus cereus in repfeds.one of the pathogens of concern in refrigerated and processed foods of extended durability (repfed) is psychrotrophic bacillus cereus, because of its ability to survive pasteurisation and grow at low temperatures. in this study a quantitative microbiological exposure assessment (qmea) of psychrotrophic b. cereus in repfeds is presented. the goal is to quantify (i) the prevalence and concentration of b. cereus during production and shelf life, (ii) the number of packages with potential emetic tox ...201324029028
[identification of a denitrifying polyphosphate-accumulating organism (dpao) and study on its denitrifying functional genes].a denitrifying polyphosphate-accumulating organism (dpao) was isolated and identified, and the denitrifying functional genes were investigated. the strain zqn2 was isolated using denitrifying medium. it was distinguished as dpao by tests of aerobic phosphorus uptake, nitrate reduction, metachromatic granules and phb (poly-beta-hydroxybutyrate) granules dyeing. test of anaerobic phosphorus release/anoxic phosphorus uptake showed that strain zqn2 released phosphorus and synthesized phb at the anae ...201324028026
chemical composition and biological activity of the essential oil from leaves of moringa oleifera lam. cultivated in mozambique.the antioxidant capacity and antimicrobial activity of the essential oil of moringa oleifera (moringaceae) grown in mozambique was investigated. the chemical composition was studied by means of gc and gc-ms analysis. hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. ultra high performance chromatography-dad analysis detected the flavonoids quercetin (126 μg/g) and luteolin (6.2 μg/g). the essential oil exhibited a relatively low free radical scavenging cap ...201324022760
durable contact active antimicrobial materials formed by a one-step covalent modification of polyvinyl alcohol, cellulose and glass surfaces.in this work we have applied a direct covalent linkage of quaternary ammonium salts (qas) to prepare a series of contact active antimicrobial surfaces based on widely utilized materials. formation of antimicrobial polyvinyl alcohol (pva-qas), cellulose (cellulose-qas) and glass (glass-qas) surfaces was achieved by one step synthesis with no auxiliary linkers. the x-ray photoelectron spectroscopy (xps) revealed tridentate binding mode of the antimicrobial agent. the antimicrobial activity of the ...201324012705
unsaturated fatty acids from food and in the growth medium improve growth of bacillus cereus under cold and anaerobic conditions.in a chemically defined medium and in luria broth, cold strongly reduced maximal population density of bacillus cereus atcc 14579 in anaerobiosis and caused formation of filaments. in cooked spinach, maximal population density of b. cereus in anaerobiosis was the same at cold and optimal temperatures, with normal cell divisions. the lipid containing fraction of spinach, but not the hydrophilic fraction, restored growth of b. cereus under cold and anaerobiosis when added to the chemically defined ...201324010589
pgiak1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the antarctic concordia station confined environment.pgiak1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant bacillaceae strain jmak1. the strain was originally isolated from the confined environments of the antarctic concordia station. analysis of the pgiak1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as w ...201324009682
structural and chemical aspects of resistance to the antibiotic fosfomycin conferred by fosb from bacillus cereus.the fosfomycin resistance enzymes, fosb, from gram-positive organisms, are m(2+)-dependent thiol tranferases that catalyze nucleophilic addition of either l-cysteine (l-cys) or bacillithiol (bsh) to the antibiotic, resulting in a modified compound with no bacteriacidal properties. here we report the structural and functional characterization of fosb from bacillus cereus (fosb(bc)). the overall structure of fosb(bc), at 1.27 å resolution, reveals that the enzyme belongs to the vicinal oxygen chel ...201324004181
synthesis and characterization of novel organocobaloximes as potential catecholase and antimicrobial activity agents.an asymmetric, potentially bidentate dioxime ligand (h₂l) was formed by condensation of 4-biphenylchloroglyoxime and napthyl-1-amine. two equivalents of h₂l were reacted with cocl₂  · 6h₂o under appropriate conditions with deprotonation of the dioxime ligand. a series of new organocobaloxime derivatives of the type [cor(hl)₂py], [corl₂pyb₂f₄], and [corl₂py(cu(phen))₂] (h₂l = 4-(napthyl-1-amino)biphenylglyoxime; phen = 1,10-phenathroline; r = izopropyl and benzyl; py = pyridine) were synthesized. ...201324003018
gene transcription from the linear plasmid pbclin15 leads to cell lysis and extracellular dna-dependent aggregation of bacillus cereus atcc 14579 in response to quinolone-induced stress.the bacillus cereus type strain atcc 14579 harbours pbclin15, a linear plasmid with similar genome organization to tectiviruses. since phage morphogenesis is not known to occur it has been suggested that pbclin15 may be a defect relic of a tectiviral prophage without relevance for the bacterial physiology. however, in this paper, we demonstrate that a pbclin15-cured strain is more tolerant to antibiotics interfering with dna integrity than the wt strain. growth in the presence of crystal violet ...201324002748
yaxab, a yersinia enterocolitica pore-forming toxin regulated by rova.the transcriptional regulator rova positively regulates transcription of the yersinia enterocolitica virulence gene inv. invasin, encoded by inv, is important for establishment of y. enterocolitica infection. however, a rova mutant is more attenuated for virulence than an inv mutant, implying that rova regulates additional virulence genes. when the y. enterocolitica rova regulon was defined by microarray analysis, ye1984 and ye1985 were among the genes identified as being upregulated by rova. si ...201324002058
metal based pharmacologically active agents: synthesis, structural characterization, molecular modeling, ct-dna binding studies and in vitro antimicrobial screening of iron(ii) bromosalicylidene amino acid chelates.in recent years, great interest has been focused on fe(ii) schiff base amino acid complexes as cytotoxic and antitumor drugs. thus a series of new iron(ii) complexes based on schiff bases amino acids ligands have been designed and synthesized from condensation of 5-bromosalicylaldehyde (bs) and α-amino acids (l-alanine (ala), l-phenylalanine (phala), l-aspartic acid (aspa), l-histidine (his) and l-arginine (arg)). the structure of the investigated iron(ii) complexes was elucidated using elementa ...201424001978
in pursuit of protein targets: proteomic characterization of bacterial spore outer layers.bacillus cereus, responsible for food poisoning, and clostridium difficile, the causative agent of clostridium difficile-associated diarrhea (cdad), are both spore-forming pathogens involved in food spoilage, food intoxication, and other infections in humans and animals. the proteinaceous coat and the exosporium layers from spores are important for their resistance and pathogenicity characteristics. the exosporium additionally provides an ability to adhere to surfaces eventually leading to spore ...201323998435
sepsis and meningoencephalitis caused by bacillus cereus in a patient with myelodysplastic syndrome.we herein report the findings of a case of myelodysplastic syndrome that was complicated by septicemia and meningoencephalitis, both of which were caused by bacillus cereus. in contrast to all of the previous cases of b. cereus that have been seen at our institution, this patient did not have any invasive devices, such as a central venous catheter, that could have acted as a conduit for a b. cereus infection. although b. cereus-induced meningoencephalitis is often lethal, the immediate treatment ...201323994997
bacillus anthracis diagnostic detection and rapid antibiotic susceptibility determination using 'bioluminescent' reporter phage.genetically modified phages have the potential to detect pathogenic bacteria from clinical, environmental, or food-related sources. herein we assess an engineered 'bioluminescent' reporter phage (wß::luxab) as a clinical diagnostic tool for bacillus anthracis, the etiological agent of anthrax. wß::luxab is able to rapidly (within minutes) detect a panel of b. anthracis strains by transducing a bioluminescent phenotype. the reporter phage displays species specificity by its inability, or signific ...201323994352
optimization of fermentation conditions for green pigment production from bacillus cereus m¹ 16 (mtcc 5521) and its pharmacological application.optimal culture conditions for the production of green pigment was investigated. the optimal culture condition for the production of an extracellular green pigment by growing bacillus cereus m(1) 16 (mtcc 5521) in a complex medium containing (g l(-1) ) peptone-4.0, beef extract-9.0, nacl-7.0, mgso4 .7h2 o-1.0 and kh2 po4 -5.0 was as follows ph-7.0 at 30°c for 72 h in a 5 l fermenter. aeration rate and agitator speed had no effect on the pigment production. thin layer chromatogram of the pigment ...201423992047
synthesis, spectral, and in vitro antibacterial studies of organosilicon(iv) complexes with schiff bases derived from amino acids.the present work stems from our interest in the synthesis, characterization, and antibacterial evaluation of organosilicon(iv) complexes of a class of amino-acid-based schiff base which have been prepared by the interaction of ethoxytrimethylsilane with the schiff bases (n oh) in 1 : 1 molar ratio. these complexes have been characterized by elemental analysis, molar conductance, and spectroscopic studies including electronic ir and nmr ((1)h, (13)c, and (29)si) spectroscopy. the analytical and s ...201323983671
cyclic dipeptides from rhabditid entomopathogenic nematode-associated bacillus cereus have antimicrobial activities.the cell free culture filtrate of bacillus cereus associated with an entomopathogenic nematode, rhabditis (oscheius) sp. exhibited strong antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain four bioactive compounds. the structure and absolute stereochemistry of these compounds were determined based on extensive spectroscopic analyses (fabms, (1)h nmr, (13)c nmr, (1)h-(1)h cosy, (1)h-(13)c hmbc) and marfey' ...201423979826
the diversity and antimicrobial activity of preussia sp. endophytes isolated from australian dry rainforests.limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of preussia within australia. this report describes endophytic preussia species that were identified through molecular analysis of the internal transcribed spacer region. screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (etoac) extracts derived from fungal mycelia against a panel of atcc type strains which included bacillus cereus, escher ...201423975673
characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage.due to changes in the design of industrial food processing and increasing international trade, highly thermoresistant spore-forming bacteria are an emerging problem in food production. minimally processed foods and products with extended shelf life, such as milk products, are at special risk for contamination and subsequent product damages, but information about origin and food quality related properties of highly heat-resistant spore-formers is still limited. therefore, the aim of this study wa ...201323973839
addition of ethanol to supercritical carbon dioxide enhances the inactivation of bacterial spores in the biofilm of bacillus cereus.supercritical carbon dioxide (sc-co2) was used to inactivate bacillus cereus spores inside biofilms, which were grown on stainless steel. sc-co2 treatment was tested using various conditions, such as pressure treatment (10-30 mpa), temperature (35-60°c), and time (10-120 min). b. cereus vegetative cells in the biofilm were completely inactivated by treatment with sc-co2 at 10 mpa and at 35°c for 5 min. however, sc-co2 alone did not inactivate spores in biofilm even after the treatment time was e ...201323973829
three different bacterial detection systems for platelet concentrates under inter-laboratory conditions.a variety of screening methods are currently used worldwide in order to decrease the risk of transfusion-transmitted sepsis and improve the safety of pcs.201323972777
evaluation of direct 16s rdna sequencing as a metagenomics-based approach to screening bacteria in bottled water.deliberate or accidental contamination of food, feed, and water supplies poses a threat to human health worldwide. a rapid and sensitive detection technique that could replace the current labor-intensive and time-consuming culture-based methods is highly desirable. in addition to species-specific assays, such as pcr, there is a need for generic methods to screen for unknown pathogenic microorganisms in samples. this work presents a metagenomics-based direct-sequencing approach for detecting unkn ...201323971801
chitosan-containing bread made using marine shellfishery byproducts: functional, bioactive, and quality assessment of the end product.chitosan is nature's second most abundant polymer after cellulose and forms the structural support in crustacean shell material and basidomycete mushroom stalks. chitosan is a known antimicrobial agent but, to date, was not examined as an antimicrobial agent in bread formulations for the prevention of mold or rope formation. the aim of this work was to investigate the effects of chitosan generated from prawn shell byproducts on the color, moisture, and texture and crumb formation of bread. a sec ...201323962198
antioxidative, antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract.this study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of leea indica leaf ethanol extract. phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, dpph radical scavenging effect, fecl3 reducing power, dmso superoxide scavenging effect and iron chelating effects were studied by established methods. antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine ...201323961238
influence of inoculation with plant growth promoting rhizobacteria (pgpr) on tomato plant growth and nematode reproduction under greenhouse conditions.numerous species of soil bacteria which flourish in the rhizosphere of plants or around plant tissues stimulate plant growth and reduce nematode population by antagonistic behavior. these bacteria are collectively known as pgpr (plant growth promoting rhizobacteria). the effects of six isolates of pgpr pseudomonas putida, pseudomonas fluorescens, serratia marcescens, bacillus amyloliquefaciens, bacillus subtilis and bacillus cereus, were studied on tomato plant growth and root knot nematode repr ...201223961220
isolation and screening of bioactive principle from chaetomorpha antennina against certain bacterial strains.microbial pathogens develop resistance to a particular antibiotic after repeated administration during the treatment of infectious diseases. moreover, multiple drug resistance is a very common problem especially in hospital acquired infections. therefore, it is necessary to find out alternative antibacterial drugs and the present trend is focused on seaweeds. this preliminary research work was carried out to find out the antibacterial activity of petroleum ether extract of chaetomorpha antennina ...201323960826
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