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[nosocomial infections in present-day traumatological-orthopedic hospitals].nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...19957626997
periodontal microbiota of mobile and non-mobile teeth.the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ...19957623258
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
clinical features and aerobic and anaerobic microbiological characteristics of cellulitis.to investigate the aerobic and anaerobic microbiological characteristics of cellulitis and correlate them with the infection site.19957611872
mapping of the immunoglobulin light chain-binding site of protein l.protein l is a cell surface protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule binds specifically and with high affinity to immunoglobulins (ig) of a wide range of animal species. the ig-binding activity is mediated through five highly homologous domains, each 72 to 76 amino acid residues long, which interact with framework regions in the variable domain of ig light chains. the interaction does not interfere with the antigen binding capac ...19957608965
antibacterial effects of some root end filling materials.in addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
factors affecting the occurrence of bacteremia associated with tooth extraction.this study examined the factors that affect the occurrence of bacteremia associated with tooth extraction and the kinds of bacteria causing this bacteremia. bacteremia was found in 132 (72.1%) of 183 patients who had one or more teeth extracted for various reasons. bacteremia occurred more frequently when teeth were extracted because of inflammatory dental diseases. the occurrence of bacteremia also increased with the number of teeth extracted and the age of the patients. when the volume of bloo ...19957594761
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
role of escherichia coli p fimbriae in intestinal colonization in gnotobiotic rats.adherence via p fimbriae is associated with long-term persistence of escherichia coli in the human large intestine, but a causal relationship has not been proven. in the present study, germfree rats were colonized with a mixture of two isogenic e. coli strains, one p fimbriated and the other type 1 fimbriated. both types of fimbriae conferred adherence to rat colonic epithelial cells. with two mutant strains from a pyelonephritogenic isolate of serotype o75:k5:h-, the p-fimbriated strain 824 att ...19957591136
the ga module, a mobile albumin-binding bacterial domain, adopts a three-helix-bundle structure.we present the first study of the secondary structure and global fold of an albumin-binding domain. our data show that the ga module from protein pab, an albumin-binding protein from the anaerobic bacterial species peptostreptococcus magnus, is composed of a left-handed three-helix bundle. the helical regions were identified by sequential and medium range noes, values of nh-c alpha h coupling constants, chemical shift indices, and the presence of slowly exchanging amide protons, as determined by ...19957589548
the role of cefotaxime in the treatment of surgical infections.this study examines the role of cefotaxime in the treatment of both gram-negative and gram-positive surgical infections. a dose of 2 g of cefotaxime will sustain peripheral compartment concentrations of 2.6, 3.9, 1.6, and 0.7 micrograms/ml for 6, 8, 10 and 12 h, respectively. therefore, the proportion of pathogens with a minimal inhibitory concentration (mic) below the peripheral compartment cefotaxime concentrations was assessed as a measure of therapeutic potential. it was observed that bacter ...19957587036
recombinant proteins l and lg: efficient tools for purification of murine immunoglobulin g fragments.in order to improve antibody purification methods, recombinant proteins l and lg were tested in the purification of murine monoclonal immunoglobulin g (igg) and its fragments. after affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. affinity chromatographic experiments confirme ...19957581856
changes in the core tonsillar bacteriology of recurrent tonsillitis: 1977-1993.microbiological studies of the core of tonsils removed from children with recurrent tonsillitis due to group a beta-hemolytic streptococci were conducted during three periods, with 50 patients in each period: 1977-1978 (period 1), 1984-1985 (period 2), and 1992-1993 (period 3). mixed flora were present in all tonsils, with 8.1 organisms per tonsil (3.8 aerobes and 4.3 anaerobes). the predominant isolates in each period were staphylococcus aureus, moraxella catarrhalis, peptostreptococcus species ...19957578726
[in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria].during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ...19957567117
in vitro activity of bay y 3118, and nine other antimicrobial agents against anaerobic bacteria.the antibacterial activity of bay y 3118, a new chlorofluoroquinolone, was determined against 257 strains of anaerobic bacteria and compared with the activities of ofloxacin, ciprofloxacin, sparfloxacin, imipenem, cefoxitin, clindamycin, chloramphenicol, metronidazole, and ornidazole. overall, bay y 3118 was the most active agent tested against the bacteroides fragilis group. its activity (mic90, 0.5 mg/l) was 16-fold lower than that of sparfloxacin (mic90, 8 mg/l), and more than 100-fold lower ...19957562012
microbiology of secondary bacterial infection in scabies lesions.aerobic and anaerobic bacteria were grown from specimens obtained from 30 children with secondarily infected scabies lesions. aerobic or facultative bacteria only were present in 14 (47%) patients, anaerobic bacteria only were present in 6 (20%) patients, and a mixed anaerobic-aerobic flora was present in 10 (33%) patients. fifty isolates were recovered (1.7 per specimen); 27 were aerobic or facultative bacteria and 23 were strict anaerobes. the predominant aerobic and facultative bacteria were ...19957559963
the microbiology and antibiotic treatment of peritonsillar abscesses.pus from 53 peritonsillar abscesses was cultured and associations between the microbiological results and clinical data were investigated with the aim of developing a clinical protocol for treatment. a positive culture grew in 85% of quinsies and of these 16% produced aerobes and 84% anaerobes. penicillin-resistant organisms were grown from 32% of patients and all but one of these organisms (haemophilus influenzae) was sensitive to metronidazole. there was no association between clinical present ...19957554331
a phage display system for studying the sequence determinants of protein folding.we have developed a phage display system that provides a means to select variants of the igg binding domain of peptostreptococcal protein l that fold from large combinatorial libraries. the premise underlying the selection scheme is that binding of protein l to igg requires that the protein be properly folded. using a combination of molecular biological and biophysical methods, we show that this assumption is valid. first, the phage selection procedure strongly selects against a point mutation i ...19957549875
foot infections in diabetic patients: the role of anaerobes.the prevalence of anaerobic bacteria in cultures of specimens from foot infections in diabetic patients is dependent upon the method of obtaining the specimen, the care with which it is transported anaerobically, and the sophistication of the laboratory methods. the rate at which anaerobes are isolated with use of the best methods ranges from 74% to 95% of patients, but it is only 41% to 53% in clinical studies of patients with limb-threatening infection. the mean number of bacterial isolates fr ...19957548576
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
role of bacterial vaginosis in pelvic inflammatory disease.pelvic inflammatory disease (pid) is a frequent infection in sexually active young women and results in adverse sequelae, including tubal-factor infertility and ectopic pregnancy. in the 1970s investigations using culdocentesis demonstrated that anaerobic bacteria played an important role in the etiology of pid. this finding has subsequently been confirmed by studies utilizing laparoscopy and/or endometrial biopsy to obtain specimens directly from the upper genital tract (uterine cavity and fall ...19957548573
a retrospective review of cases of anaerobic empyema and update of bacteriology.we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ...19957548560
bacteriology of chronic otitis media, chronic sinusitis, and paranasal mucopyocele in japan.a bacteriologic study of cases of chronic otitis media, chronic sinusitis, and paranasal mucopyocele diagnosed at gifu university hospital was performed between 1989 and 1992. bacteria were isolated from all of 13 samples of discharge from the ears of patients with chronic otitis media, all of 14 mucosal biopsy specimens from patients with chronic sinusitis, 8 of 10 aspirate samples from patients with chronic sinusitis, and 9 of 15 aspirates samples from patients with mucopyocele. cultures often ...19957548557
role of the reference laboratory in susceptibility testing of anaerobes and a survey of isolates referred from laboratories in england and wales during 1993-1994.methods for susceptibility testing of anaerobes and their patterns of susceptibility remain debatable. the responsibilities of the public health laboratory service anaerobe reference unit include identification of referred isolates; development of methods; monitoring of the occurrence of anaerobic infections; performance of taxonomic studies; maintenance of a reference collection; and monitoring of the antibiotic susceptibility of clinical isolates. selection of antibiotics for anaerobic infecti ...19957548547
translation of trpg in bacillus subtilis is regulated by the trp rna-binding attenuation protein (trap).trpg of bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and l-tryptophan. expression of trpg is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. examination of both transcriptional and translational gene fusions to lacz involving trpg and direct measurements of trpg mrna levels and trpg polypeptide accumulation demonstrated that translation of trpg is regulated by trypt ...19957543470
techniques for controlling variability in gram staining of obligate anaerobes.identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...19957538512
in vitro comparison of the effectiveness of three surface disinfectants.choosing a general purpose surface disinfectant for the dental office environment is difficult because of the wide range of products available, varying claims by manufacturers and contradictory reports in the literature regarding product efficacy. this study tested the antimicrobial effectiveness of diluted-for-use o-phenyl-phenol (omni), didecyl dimethyl ammonium (basic g) and isopropanol (virahol). products were tested for antimicrobial activity at 0, 12 and 24 hours, 3, 6 and 8 days. o-phenyl ...19947523623
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
clinical and microbiological features of necrotizing fasciitis.the microbiological and clinical characteristics of 83 patients with necrotizing fasciitis (nf) treated over a period of 17 years are presented. bacterial growth was noted in 81 of 83 (98%) of specimens from patients with nf. aerobic or facultative bacteria only were recovered in 8 (10%) specimens, anaerobic bacteria only were recovered in 18 (22%) specimens, and mixed-aerobic-anaerobic floras were recovered in 55 (68%) specimens. in total, there were 375 isolates, 105 aerobic or facultative bac ...19957494032
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...19947478759
[a case of gas-producing brain abscess with subdural empyema: timing of the operation for brain abscess].a case of gas-producing brain abscess with subdural abscess was reported. an 18-year-old boy was admitted with a five-day history of vomiting and high grade pyrexia. plain skull roentgenograms demonstrated left frontal multiple gas bubbles. ct scan and mri showed that both brain and subdural abscesses contained gas in the left frontal area. antibiotics and glyceol were intravenously administrated. in serial ct scans, subdural abscess was not recognized, while brain abscess was enhanced in a ring ...19957477705
[pathology caused by anaerobic bacteria in pediatrics. our cases]. 19807453681
[endocarditis due to the anaerobe peptostreptococcus]. 19807450583
[bacteriological examinations of liquid drawn from the pouch of douglas (author's transl)].the article reports on bacteriological examinations of 510 samples of liquid drawn from the pouch of douglas (excavatio rectouterina) during pelviscopy in patients not displaying any signs of inflammation. bacterial populations were found in 270 samples (= 52.9%). the results are subdivided according to aerobic and anaerobic types of pathogens and their incidence.19807450495
anaerobic and aerobic bacteriology of decubitus ulcers in children.forty-two children with decubitus ulcers were studied using aerobic and anaerobic techniques. anaerobic bacteria were isolated in 21 (50%) of the patients, five times as the only isolates and 16 times mixed with aerobes. aerobes only were present in 20 (48%) of the patients. there were a total of 83 isolates, 46 aerobes and 37 anaerobes, with an average of two species per specimen (1.1 aerobes and 0.9 anaerobes). the predominant anaerobic isolates were gram-positive cocci (17), bacteroides fragi ...19807436139
[pleuro-pulmonary infections with anaerobic germs (author's transl)].the author presents his experience with the study of 42 patients with pleuro-pulmonary infection with anaerobic germs. he underlines the value of research tending to improve the conditions of sampling and culturing to ensure a bacteriological diagnosis and the setting of a suitable therapy.19807433351
effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria.the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ...19807431377
bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions.streptococcus sanguis and peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. the bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100.19807429633
agglutinins to bacteria in crohn's disease.sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ...19807429299
['sterile' brain abscesses (author's transl)].in 16 cases of brain abscess (1970 to 1976) 10 could be identified to be bacteriologically sterile. however in 5 cases of "sterile" cerebral abscesses, where swabs were taken under anaerobic conditions, it was possible to isolate anaerobic bacteria in every case. it is concluded that in reality thse so-called "sterile" brain abscesses were also contaminated with anaerobes.19807415618
aerobic and anaerobic bacteriology of cervical adenitis in children.needle aspirates from 53 inflamed cervical lymph glands were studies for aerobic and anaerobic bacteria and mycobacteria. bacterial growth was achieved in 45 patients (85%). sixty-six bacterial isolates were recovered, averaging 1.5 isolates per specimen (0.8 aerobes and 0.7 anaerobes), with as many as 4 isolates in some specimens. aerobic organisms alone were recovered in 27 aspirates (60%) of the 45 culture-positive aspirates, anaerobic bacteria alone in 8 (18%), and mixed aerobic and anaerobi ...19807408372
bacterial studies of peritoneal cavity and postoperative surgical wound drainage following perforated appendix in children.this study reports bacterial specimens obtained from 112 children presenting with a ruptured appendix. additional samples were studied from 11 of these patients who developed a postoperative surgical draining wound. bacterial growth occurred in 100 peritoneal fluid specimens. anaerobic bacteria alone were present in 14 specimens, aerobes alone in 12, and mixed aerobic an anerobic flora in 74 specimens. there were 144 aerobic isolates (1.4 per specimen). the predominant isolates were: e. coli (57 ...19807406576
isolation, synthesis, and properties of 8-hydroxyflavins. 19807374472
determination of fmn and fad by fluorescence titration with apoflavodoxin. 19807374468
fatal aspiration pneumonia in an african elephant. 19817328011
[the absorption, excretion and influence on bowel flora of oral paromomycin sulfate (author's transl)].the absorption, excretion and influence on bowel flora of oral paromomycin sulfate (aminosidine, prm) were studied in ten normal volunteers taking a normal diet, and the following results were obtained. 1. serum levels of prm were observed 0.46 micrograms/ml at a half hour, 1.14 micrograms/ml at 1 hour, 1.48 micrograms/ml at 2 hours, 0.70 micrograms/ml at 4 hours, 0.29 micrograms at 6 hours and were almost faded out at 12 hours after 4 grams of oral administration. 2. during 0 approximately 2 ho ...19817321186
extensive botryomycosis in a patient with diabetes and chronic active hepatitis.extensive botryomycosis was diagnosed in a patient with diabetes mellitus with chronic active hepatitis. peptostreptococcus organisms and staphylococcus aureus grew from the sulfur granule cultures. the patient's neutrophils showed notable inhibition of chemotaxis toward endotoxin-activated normal serum. the patient's serum also had an inhibitor to neutrophil chemotaxis, this inhibiting substance was probably antistreptolysin o. results of other tests of neutrophil function and skin tests to eva ...19817316536
case report: livedo reticularis in endocarditis.the peripheral manifestations of bacterial endocarditis are manifold and provide diagnostic clues as to the onset, etiology and potential complications of the disease. petechiae, splinter hemorrhages, and osler's nodes are among the more common cutaneous signs of endocarditis thought to be secondary to an allergic vasculitis or, as in the case of osler's nodes, peripheral emboli. an asymmetric livedo reticularis rash was the initial presentation in a patient we recently treated with peptostrepto ...19817315865
[complication following the vaginal use of potassium permanganate for an abortion].complications after abortifacient use of potassium permanganate in a 22-year old woman are described. approximately 1 month prior to admission, the patient placed in the vagina a bag with potassium permanganate for 12 hours. gynecological examination showed stenosis of the vagina, enlargement of the uterus, and adhesions in the cervix and vagina. the patient underwent surgical separation of the vagina, daily irrigations of the vagina, and postoperative administration of ampilillin. microbiol ...19817304855
adrenal abscess in a neonate due to gas-informing organisms: a diagnostic dilemma.a case of adrenal abscess due to bacteroides and peptostreptococcus in a 16-day-old infant is presented. the clinical manifestations consisted of a huge abdominal swelling associated with fever and lethargy. this is the first case report of an adrenal abscess due to gas-forming organisms, a finding which makes the preoperative diagnosis more difficult. surgical drainage, subtotal excision of the abscess wall under cover of antibiotics and preservation of the ipsilateral kidney were followed by s ...19817282048
aerobic and anaerobic bacteria in tonsils of children with recurrent tonsillitis.tonsils were obtained from 50 children suffering from recurrent tonsillitis. patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. the tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. there were 207 anaerobes isolated. the pre ...19817271131
[infectious peptostreptococcus endocarditis after heart catheterization]. 19817266360
antibodies to eubacterium and peptostreptococcus species and the estimated probability of crohn's disease.anaerobic coccoid rods belonging to species of eubacterium and peptostreptococcus agglutinate more frequently with sera from patients with crohn's disease than with sera from patients suffering from other diseases and from healthy subjects. results of agglutination test with four strains of coccoid anaerobes were used to estimate the probability that a patient suffers from crohn's disease. the data on healthy subjects and patients with crohn's disease were subjected to logistic discriminant anal ...19817252136
agglutination of eubacterium and peptostreptococcus species as a diagnostic test for crohn's disease.the anaerobic fecal flora of patients with crohn's disease has been found to differ from the flora of healthy subjects by higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods. agglutinating antibodies against strains of coccoid rods, belonging to species of eubacterium and peptostreptococcus were detected in much higher percentages of sera from patients with crohn's disease than in those from patients with ulcerative colitis, and healthy subjects. on the basis of the res ...19817250896
characterization of the cecal bacteria of normal pigs.one hundred ninety-two isolates from cecal contents of three normal weaned pigs were obtained by means of anaerobic roll tube methods and were characterized. seventy-eight percent of the isolates were gram-negative. the most numerous species isolated from each of the pigs was bacteroides ruminicola. this species accounted for 35% of the isolates that were characterized, and selenomonas ruminantium accounted for 21% of the isolates. other gram-negative bacteria isolated from all three pigs were b ...19817235711
a possible role of eubacterium and peptostreptococcus species in the aetiology of crohn's disease.the faecal flora of patients with crohn's disease has been found to contain higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods than the flora of healthy subjects. in a considerable percentage of sera from patients with crohn's disease, agglutinating antibodies were found against four strains of the coccoid rods, in contrast to sera from patients with ulcerative colitis, other diseases and healthy subjects. in the present study, coccoid strains c18, me46 and me47 were f ...19807235691
studies of anaerobic bacteria in chronic otitis media.in the 760 cases of chronic otitis media studied from 1970 to 1976, the detection ratio of anaerobic bacteria and the relation between the nature of anaerobic bacteria and the conditions of focus were investigated. in 8.2% of 760 cases 9 kinds and 81 strains of anaerobic bacteria was detected. most of the anaerobic bacteria were peptococcus sp. or bacteroides sp. and in general non-sporogenic anaerobic bacteria accounted for 97.5%. anaerobes were frequently detected in cases where the middle ea ...19817231031
production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins by anaerobic gram-positive cocci.clinical isolates of peptococcus and peptostreptococcus species and streptococcus intermedius strains were obtained from local hospitals. after confirmed identification, each isolate was tested for the in vitro production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins. of the 60 strains studied, 18 had enzymatic activity. the variability of enzyme production suggests that such assays are not suitable as an aid to identification of these organisms.19817229018
production of p-hydroxyhydrocinnamic acid from tyrosine by peptostreptococcus anaerobius.peptostreptococcus anaerobius was found to metabolize tyrosine to p-hydroxyhydrocinnamic acid [3-(p-hydroxyphenyl)propionic acid]. this acid was detected in spent growth media by gas-liquid chromatography, and its identity was confirmed by mass spectrometry.19807229012
management of an anaerobic infection in a prosthetic knee with long-term antibiotics alone: a case report.a 74-year-old woman had a spherocentric arthroplasty performed on her left knee. after four postoperative weeks, she developed and swelling in the joint. an anaerobic streptococcus was isolated from a joint aspirate. the patient was treated solely with knee immobilization and intravenous penicillin g for five weeks. joint aspirates at the end of treatment and six months posttreatment were sterile. however, after ten posttreatment months, the peptostreptococcal infection recurred. sterile joint a ...19817226607
animal model for anaerobic lung abscess.there are no satisfactory animal models for the study of anaerobic lung abscess. aspiration of food, gastric mucin, or hydrochloric acid, or any combination of these, along with oropharyngeal bacteria, is commonly believed to cause aspiration pneumonia and lung abscess. in the animal model described, none of the adjuvants was effective in producing anaerobic lung abscesses. anaerobic bacteria derived from dental scrapings of a healthy adult (peptococcus morbillorum, fusobacterium nucleatum, euba ...19817216463
on the formation of 3-phenylpropionate and the different stereo-chemical course of the reduction of cinnamate by clostridium sporogenes and peptostreptococcus anaerobius. 19817202724
the assay on a defined medium of the effects of beta-2-thienylalanine on the growth of anaerobic bacterial isolates from phenylketonuric patients.faecal samples were taken from three diet-managed phenylketonuric children to determine effects of beta-2-thienylalanine (beta-2-t) on indigenous bacteria. from sample swabs, 127 anaerobes were identified and tested for beta-2-t inhibition on a phenylalanine (phe)-free medium, anaerobe inhibition test (ait) agar. of the isolates, 77.9% grew sufficiently to assay reactions on at least 25% of ait plates. using phe-containing columbia agar, 86.5% of the strains could be assayed. none of 28 bacteroi ...19807189815
response of mice to transtracheal pulmonary inoculation of anaerobic bacteria.the transtracheal inoculation of a mixture of anaerobic bacteria, with or without hydrochloric acid, into the lungs of mice caused nonlethal pneumonia and abscesses in a minority of challenged animals. all animals cleared organisms and recovered by 30 days. secondary infection with escherichia coli was common but did not affect the response to challenge with the anaerobes.19827176528
[the effect of tinidazole on the anaerobic bacterial flora in patients undergoing gynecological surgery].the various effects have been examined against the anaerobes by administering tinidazole when the operations of gynecology and obstetrics and the following results were obtained. 1. the serum concentrations after the single administration of 1,000 mg of tdz showed 56.6 micrograms/ml of the peak value (3 hours) and 27.7 micrograms/ml of 24 hours value, and the antibacterial potency was maintained for a long duration. the concentration in the vaginal secretions was 21.8 micrograms/tampon within 24 ...19827176070
biotransformation of 1-nitropyrene in intestinal anaerobic bacteria.mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ...19827167066
isolation and characterisation of bacteria from pyothorax (empyaemia) in cats. 19827164339
endometrial bacteriology in patients with endometrial cancer before and after primary intracavitary irradiation using ir-192 and an afterloading technique.in a prospective clinical and bacteriological study the endocervical and endometrial flora of 38 patients with biopsy proven cancer of the endometrium was studied before and after intrauterine irradiation with 10 gy (ir-192, afterloading device, buchler, braunschweig). bacteriological swabs were taken transcervically. anaerobic transport media were used and cultures were done aerobically and anaerobically. the mean number of aerobic organisms per patient increased from 2.13 to 2.49 (not signific ...19827149781
direct quantitative determination of acidic end products in clinical specimens for presumptive diagnosis of anaerobic infections.acidic metabolic products determined directly in clinical specimens of 51 anaerobic and 12 aerobic infections by quantitative gas-liquid chromatography (glc) were correlated with the bacteriologic findings. the presence of appreciable amounts of succinic acid, more than 1 microm per ml, propionic and isovaleric acid, at concentrations not exceeding 3 microm per ml, was strong evidence for bacteroides fragilis infections. when isobutyric acid was also detected, a b. melaninogenicus infection coul ...19827149527
a bacteriological and/or immunological study of anaerobic streptococcal infection in chronic tonsillitis, paratonsillitis and periodontitis patients.anaerobic streptococci were isolated from 100% of tonsil tissue samples obtained from 36 chronic tonsillitis patients. the strains displayed antigenic and other differences. sera of chronic tonsillitis, paratonsillitis and periodontitis patients exhibited significantly more often, and in significantly higher titres, antibodies to the anaerobic streptococci isolated from tonsils than did sera of normal persons.19827142691
evaluation of a metronidazole disk test for the presumptive identification of anaerobes.a total of 632 bacterial strains recovered under anaerobic conditions from clinical specimens were tested from their susceptibility to metronidazole by a disk diffusion test using 5 micrograms metronidazole disks. three-hundred-fifty-five of the 632 bacterial strains exhibited susceptibility the metronidazole, and each was determined to be an obligate anaerobe. the remaining 277 isolates showed resistance to the 5 micrograms disk. of these resistant strains, 257 were determined to be facultative ...19827124785
analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.a standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an aminex hpx-87 column. the acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 n naoh. the presence of fumaric acid in culture extracts of peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fra ...19827119103
complete amino acid sequence of the 4fe-4s, thermostable ferredoxin from clostridium thermoaceticum.the complete amino acid sequence of the 4fe-4s ferredoxin from the thermophilic bacterium clostridium thermoaceticum has been determined. the protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4fe-4s] cluster. the sequence totals 63 residues and includes the first tryptophan (trp-26) reported for a clostridial ferredoxin, and other amino acids not commonly found in clostridial or clostridial-like ferredoxins: methionine (met-1), histidine (his-33) ...19827115670
etiologic factors in acute suppurative thyroiditis. 19827108286
anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy.human epithelium was cultured to characterize differences in microbial populations between regions of normal colon and between polyps, inflammatory bowel disease, and cancer and their respective adjacent normal mucosa. twenty-one patients (12 polyps, 5 inflammatory bowel disease, 4 cancer) underwent colonoscopy with anaerobic culture of mucosal biopsies from normal and diseased ascending, transverse, descending, and sigmoid colon. no differences for total number of organisms and recovery of spec ...19827103479
factors determining the occurrence of serum agglutinins to eubacterium and peptostreptococcus species in patients with crohn's disease and ulcerative colitis.recently, the demonstration of serum agglutinins to eubacterium and peptostreptococcus strains has been found to be useful as a diagnostic test for crohn's disease. therefore, conditions determining the occurrence of these antibodies were studied in patients with crohn's disease and ulcerative colitis. localization of crohn's disease in the colon, the presence of fistulae and serum levels of immunoglobulins were found to be contributory determinants for the occurrence of the agglutinins.19827095311
streptococcus pneumoniae and three species of anaerobic bacteria in experimental otitis media in guniea pigs.three species of anaerobic bacteria commonly isolated in upper respiratory tract infections in humans were studied in an animal model of otitis media. the ability of propionibacterium acnes to induce acute otitis media was comparable with that of streptococcus pneumoniae, the classic middle ear pathogen. the response to peptostreptococcus micros was weak and irregular, and no effects of bacteroides asaccharolyticus were observed despite the use of high doses (10(8) colony-forming unite per ear). ...19827086197
intracranial complications of sinusitis in children. a sequela of periapical abscess.the cases of two children with periapical abscess in the upper incisors, sinusitis, and intracranial abscess are described. the ethmoid and maxillary sinuses were involved in both patients. subdural empyema occurred in both, and one of the children had also cerebritis and brain abscess. anaerobic bacteria were isolated from the infected subdural empyemas. peptostreptococcus intermedius and microaerophilic streptococci were recovered in one patient and fusobacterium sp in the other. surgical drai ...19827073175
evidence that the greening ligand in native butyryl-coa dehydrogenase is a coa persulfide.yellow butyryl-coa dehydrogenase and general acyl-coa dehydrogenase are "greened" by a mixture of coenzyme a plus elemental sulfur. the resultant stable complex contains an identical ligand with that present in native green butyryl-coa dehydrogenase and has the same broad absorption band centered at 710 nm. evidence is presented that the greening ligand is a coa persulfide, possibly a mimic of the substrate carbanion thought to be generated early in the normal catalytic cycle. variation in the p ...19827068637
[endometrial bacteriology in puerperal infections (author's transl)].from the third to the sixth day postpartum transcervical endometrial swabs were taken from 51 women with the clinical signs of endometritis, like fever over 38 degree c, uterine tenderness and purulent lochia using prereduced anaerobic transport-media. the specimens were cultured aerobically and anaerobically. 27 women had an endometritis following vaginal delivery, 24 following caesarian section. following vaginal delivery aerobic bacteria were isolated in 85.2% of the cases and anaerobic bacte ...19817032100
colonic bacteria: a source of gamma-aminobutyric acid in blood. 19817019921
serum agglutinins to eubacterium and peptostreptococcus species in crohn's and other diseases.sera from patients suffering from crohn's and other diseases and from healthy subjects were tested for agglutinins to anaerobic, gram-positive coccoid rods belonging to species of eubacterium and peptostreptococcus. four strains labelled eubacterium contortum (two strains), eubacterium rectale and peptostreptococcus productus were agglutinated by a higher percentage of sera from patients with crohn's disease than from healthy subjects and from patients with liver and intestinal diseases (includi ...19817019318
the faecal flora of patients with crohn's disease.the faecal flora of patients with crohn's disease was compared with that of healthy subjects. in patients with terminal ileitis, numbers of anaerobic gram-negative and coccoid rods (species of eubacterium and peptostreptococcus) were higher than in the controls whereas anaerobic gram-positive rods and cocci and aerobes occurred in normal numbers. the composition of the flora was neither influenced by duration of the disease nor by ileocaecal resection. in healthy subjects and patients, a chemica ...19817019315
[saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)].a microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. this group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic streptococci " and bacteroides, followed by fusobacterium and veillonella. no propionibacterium, eubacterium or bifidobacterium have been isolated. according to many authors the non-spore form ...19807018415
anaerobic bacteria in pediatric respiratory infection: progress in diagnosis and treatment. 19817017948
premature labor. ii. bacterial sources of phospholipase.human term labor is thought to be initiated by amniotic and chorionic phospholipase a2, an enzyme that liberates arachidonic acid esters from the phospholipids of these membranes, leading to the synthesis of prostaglandins by the placental membranes. the striking association of premature labor with intrauterine infection or contamination, urinary tract infection, and early neonatal sepsis led us to study the microorganisms present in these infections for phospholipase a2 activity. activity was f ...19817017516
puerperal sepsis in port moresby, papua new guinea.because puerperal sepsis is the second most common cause of maternal death in papua new guinea some aspects of its aetiology and prevention have been investigated. 91% of 125 women with puerperal fever were found to have post-partum uterine infection. the anaerobes bacteroides and peptostreptococcus singly or together, were the commonest causes, while streptococcus pyogenes was the predominant aerobic species. antibiotic sensitivity patterns were established, and chloramphenicol was found to be ...19807008426
microbial flora of the lower genital tract of women in labour in zaria, nigeria.nine genera of microbes isolated from the lower genital tract of 187 women in labour in zaria have been identified. the work was undertaken to establish the nature of microorganisms in the lower genital tract of women in labour as a basis for further study. the isolates in order of prevalence were: candida albicans (20.9%), klebsiella sp (15.0%), escherichia coli (9.1%), streptococcus faecalis (6.4%), haemolytic streptococci (other than streptococcus pyogenes (2.7%), streptococcus viridans (2.1% ...19817007447
introduction of anaerobic methodology into a clinical microbiological laboratory.for the successful introduction of anaerobic methodology into a clinical microbiological laboratory, several factors are very important. these include: 1) laboratory; 2) establishment of skilful technicians and adequate equipment; and 3) communication with clinicians who believe in anaerobes as infectious agents and are interested in working with the microbiologist. the beneficial effects of these factors on a laboratory's efficiency in providing an anaerobic service are demonstrated by the foll ...19807005099
microorganisms associated with abscesses of sheep and goats in the south of iran.a total of 86 abscesses (45 and 41) abscesses of sheep and goats, respectively) were examined for their causal agents; 44 of these abscesses were located subcutaneously, and the remaining 42 were in lungs, livers, intestines, and udders. a total of 23 different types of microorganisms were isolated from 78 abscesses; bacteria were not detected in the remaining eight abscesses. microorganisms isolated were: species of the genera corynebacterium, staphylococcus, and streptococcus and pasteurella, ...19806996545
microorganisms isolated from peritonsillar abscess and indicated chemotherapy.a bacteriologic study was made of 30 patients with peritonsillar abscesses with the use of both aerobic and anaerobic culture procedures. the abscess was punctured and the pus was aspirated by a syringe with an 18-gauge needle. aerobes and anaerobes were detected in a frequency rate of approximately 1:2. peptostreptococcus (30.2%), group a streptococci (27.9%), peptococcus (16.3%), and fusobacterium (9.3%) were preponderant pathogens. anaerobic organisms were isolated in 75% of 30 cases. aside f ...19826982036
identification of attached bacteria in the nasopharynx of the child.bacteria attached to epithelial cells in mucus and cell scrapings obtained from the nasopharynx in children undergoing adenoidectomy appear rather monomorphic under the microscope, and for this reason it is not possible to identify different species. to overcome this difficulty, a micromanipulation method has been devised which allows the isolation of single squamous epithelial cells seen to carry bacteria. the cells are transferred to culture media for bacteriological analysis. ciliated epithel ...19826980192
[studies on in vitro susceptibility of various anaerobic bacteria and antimicrobial resistance of anaerobes (author's transl)]. 19826979640
[clinico-microbiologic bases for the use of new cephalosporins]. 19826979006
[experimental study of the pathogenicity of anaerobic streptococci (peptostreptococci)]. 19816977248
bacteriologic study of paronychia in children. 19816972709
[assessment of the leukocyte migration inhibition test in response to selected bacterial antigens from dental focal infections]. 19826964137
[evaluation of humoral response to selected bacterial antigens in dental focal infections]. 19826964085
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