Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| impact of acid on survival of vibrio vulnificus and vibrio vulnificus phage. | three strains of vibrio vulnificus and v. vulnificus phages were tested for acid sensitivity at 21 degrees c. v. vulnificus strain 304 was more resistant to ph 4.0 than strains cvd-1 and a-9, whereas acid sensitivities of v. vulnificus strains at ph 3.0 and 2.0 were similar. v. vulnificus phage strain 110a-7 was more resistant to ph 4.0 than strain 153a-7, whereas acid sensitivities of phage strains at ph 3.5 and 3.0 were similar. numbers of v. vulnificus and its phage were close to the limit of ... | 2000 | 10945579 |
| cloning, sequencing and expression of the gene encoding the extracellular metalloprotease of aeromonas caviae. | a gene (apk) encoding the extracellular protease of aeromonas caviae ae6 has been cloned and sequenced. for cloning the gene, the dna genomic library was screened using skim milk lb agar. one clone harboring plasmid pkk3 was selected for sequencing. nucleotide sequencing of the 3.5 kb region of pkk3 revealed a single open reading frame (orf) of 1,785 bp encoding 595 amino acids. the deduced polypeptide contained a putative 16-amino acid signal peptide followed by a large propeptide. the n-termin ... | 2000 | 10888351 |
| protective effect of c-reactive protein against the lethality induced by vibrio vulnificus lipopolysaccharide. | vibrio vulnificus infection has attracted special interest because of its high mortality. a strong clinical association exists between hepatic dysfunction and increased morbidity and mortality from v. vulnificus infection. in this study, the effect of c-reactive protein (crp), a typical hepatogenic acute phase protein, on the lethality induced by v. vulnificus lipopolysaccharide (lps) was investigated in galactosamine-sensitized mice. the pretreatment of crp, in a dose of at least 2 mg/kg, 2 hr ... | 2000 | 10888350 |
| evaluation of a simplified semi-quantitative protocol for the estimation of vibrio vulnificus in bathing water using cellobiose-colistin agar: a collaborative study with 13 municipal food controlling units in denmark. | a simplified semi-quantitative method using pre-enrichment in alkaline peptone water supplemented with polymyxin b and plating onto cellobiose-colistin (cc) agar for the estimation of vibrio vulnificus in bathing water was evaluated. this protocol was tested in a collaborative study with 13 food controlling laboratories in denmark during the 1999 bathing season in periods when water temperatures exceeded 20 degrees c. the average percentage of yellow colonies larger than 1 mm in diameter on cc a ... | 2000 | 10856777 |
| vibrio vulnificus has the transmembrane transcription activator toxrs stimulating the expression of the hemolysin gene vvha. | in an attempt to dissect the virulence regulatory mechanism in vibrio vulnificus, we tried to identify the v. cholerae transmembrane virulence regulator toxrs (toxrs(vc)) homologs in v. vulnificus. by comparing the sequences of toxrs of v. cholerae and v. parahaemolyticus (toxrs(vp)), we designed a degenerate primer set targeting well-conserved sequences. using the pcr product as an authentic probe for southern blot hybridization, a 1.6-kb bglii-hindiii fragment and a 1.2-kb hindiii fragment con ... | 2000 | 10852871 |
| renal failure in vibrio vulnificus infection. | vibrio vulnificus infection with septicemia is a life threatening disease in the immunocompromised hosts. renal involvement has not been documented. we reported herein 8 patients with v. vulnificus septicemia. all were immunocompromised hosts. four patients had cirrhosis of the liver, 3 were heavy alcohol drinkers and one had systemic lupus erythematosis. presenting symptomatology included fever, chills, leg pain and skin rash. renal failure was observed in 6 patients. four patients died shortly ... | 2000 | 10843244 |
| microbial pathogens with impaired ability to acquire host iron. | successful microbial pathogens must be adept in obtaining growth-essential iron from healthy hosts. some potential pathogens, however, are sufficiently impaired in iron acquisition ability so as to be dangerous mainly in hosts with such iron loading conditions as alcoholism, asplenia, hemochromatosis, beta-thalassemia major, or tobacco smoking. the association of six impaired pathogens (capnocytophaga canimorsis, yersinia enterocolitica and y. pseudotuberculosis, vibrio vulnificus, tropheryma wh ... | 2000 | 10831229 |
| a case of fatal food-borne septicemia: can family physicians provide prevention? | vibrio vulnificus, a common bacteria found in undercooked seafood and seawater, is the leading cause of food-borne death in florida. fatal cases of v vulnificus infection have also been reported in most states. | 2000 | 10826868 |
| molecular typing of vibrio vulnificus isolated from clinical specimens by pulsed-field gel electrophoresis and random amplified polymorphic dna analysis. | primary v. vulnificus septicemia has been continuously reported in korea. we analyzed the molecular diversity of v. vulnificus strains isolated from clinical specimens using pulsed-field gel electrophoresis (pfge) and random amplified polymorphic dna (rapd) analysis. we analyzed a total of 23 v. vulnificus strains isolated from 22 patients between 1992 and 1997. rapd analysis was performed using five random primers, and we obtained chromosomal dna restriction patterns with noti and sfii by pfge. ... | 1999 | 10816612 |
| metalloprotease is not essential for vibrio vulnificus virulence in mice. | previous work suggested that a metalloprotease, vvp, may be a virulence factor of vibrio vulnificus, which causes severe wound infection and septicemia in humans. to determine the role of vvp in pathogenesis, we isolated an isogenic protease-deficient (pd) mutant of vibrio vulnificus by in vivo allelic exchange. this pd mutant was as virulent as its parental strain in mice infected intraperitoneally and was 10-fold more virulent in mice infected via the oral route. furthermore, the pd mutant was ... | 2000 | 10816513 |
| the marine pathogen vibrio vulnificus encodes a putative homologue of the vibrio harveyi regulatory gene, luxr: a genetic and phylogenetic comparison. | vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. we have cloned and sequenced a gene from v. vulnificus that is a homologue of the positive transcriptional regulator, luxr, of the lux operon in vibrio harveyi. this gene encodes a putative, single complete open reading frame designated smcr, which shares greater than 75% nucleotide identity with luxr of v. harveyi. the deduce ... | 2000 | 10806366 |
| vibrio vulnificus infection complicated by acute respiratory distress syndrome in a child with nephrotic syndrome. | a 9-year-old girl with nephrotic syndrome visited a local hospital after developing fever, chills, and edematous changes and multiple hemorrhagic bullae on both legs over 2 days. cultures of blood and an aspirate from the bullae yielded vibrio vulnificus. the patient was transferred to our hospital because of persistent fever, generalized edema, acute renal failure, and disseminated intravascular coagulopathy. we treated this patient as a v. vulnificus infection complicated with necrotizing fasc ... | 2000 | 10790253 |
| cluster analysis of ap-pcr generated dna fingerprints of vibrio vulnificus isolates from patients fatally infected after consumption of raw oysters. | arbitrarily-primed-polymerase chain reaction (ap-pcr) dna fingerprints were generated for 10 vibrio vulnificus strains isolated from patients who became infected and died between 1993 and 1996 as a result of consuming raw oysters. analysis of the dna fingerprints with gel imaging and cluster analysis software revealed significant genetic heterogeneity among these strains, suggesting that v. vulnificus has a high degree of variation in its genomic organization, and that multiple pathogenic strain ... | 2000 | 10747262 |
| epidemiology and pathogenesis of vibrio vulnificus. | vibrio vulnificus is capable of causing severe and often fatal infections in susceptible individuals. it causes two distinct disease syndromes, a primary septicemia and necrotizing wound infections. this review discusses the interaction of environmental conditions, host factors, and bacterial virulence determinants that contribute to the epidemiology and pathogenesis of v. vulnificus. | 2000 | 10742690 |
| fulminating septicaemia caused by vibrio vulnificus. | 2000 | 10730788 | |
| a selective medium and a specific probe for detection of vibrio vulnificus. | a selective medium (vvm) and a specific 16s rrna gene (rdna) probe (v3vv) for the detection of vibrio vulnificus were developed. the medium contains d-(+)-cellobiose as the main carbon source and electrolytes (mgcl(2)-6h(2)o and kcl), which stimulate bacterial growth. polymyxin b, colistin, and moderate alkalinity and salinity provide selectivity properties. v. vulnificus grows on vvm as flat, bright yellow colonies. other vibrio species tested either did not grow or showed green-bluish colonies ... | 2000 | 10653765 |
| [studies on the zinc metalloprotease produced by vibrio vulnificus]. | 1999 | 10643296 | |
| cloning and characterization of vuua, a gene encoding the vibrio vulnificus ferric vulnibactin receptor. | the ability of vibrio vulnificus to acquire iron from the host has been shown to correlate with virulence. many iron transport genes are regulated by iron, and in v. vulnificus, transcriptional regulation by iron depends on the fur gene. the n-terminal amino acid sequence of a 72-kda iron-regulated outer membrane protein purified from a v. vulnificus fur mutant had 53% homology with the first 15 amino acids of the mature protein of the vibrio cholerae vibriobactin receptor, viua. in this report, ... | 2000 | 10639413 |
| vibrio vulnificus corneal ulcer: rapid resolution of a virulent pathogen. | to describe a corneal ulcer due to vibrio vulnificus that resolved rapidly with antibiotic therapy alone. | 2000 | 10632019 |
| [a case of a sudden death from vibrio vulnificus septicemia in a patient with liver cirrhosis]. | a 46-year-old male patient with alcoholic cirrhosis of the liver was carried to our out-patient clinic as he had developed shock while under routine follow-up, and died on the way to the hospital. he had been admitted several times since the diagnosis eight years ago, and was finally discharged from the hospital six weeks ago with improved physical condition and laboratory findings. a vesicle and bulla formation with phlegmon on the skin of right leg and sole of foot was noticed. vibrio vulnific ... | 1999 | 10624097 |
| evidence that expression of the vibrio vulnificus hemolysin gene is dependent on cyclic amp and cyclic amp receptor protein. | glucose repressed hemolysin production in vibrio vulnificus. promoter activity of the hemolysin gene, vvh, assessed with a vvh-luxcdabe transcriptional fusion, required cyclic amp (camp) and camp receptor protein (crp) in escherichia coli. hemolysin production in v. vulnificus increased after the addition of camp and was undetectable in a putative crp mutant, suggesting that vvh is also regulated by camp-crp in v. vulnificus. | 1999 | 10601227 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| effects of a commercial heat-shock process on vibrio vulnificus in the american oyster, crassostrea virginica, harvested from the gulf coast. | oysters (crassostrea virginica) harvested from the gulf coast, containing 10(2) to 10(4) most probable number (mpn) per gram of vibrio vulnificus, were subjected to a commercial heat-shock process. after 1 to 4 min at internal oyster meat temperatures exceeding 50 degrees c, shellstock oysters were shucked, chilled, washed, and packed. v. vulnificus and total bacterial levels in gulf coast oysters were significantly reduced from 1 to 4 logs in the finished product. similar reductions were not ob ... | 1999 | 10571315 |
| clinical, epidemiological, and microbiological features of vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in israel. israel vibrio study group. | vibrio vulnificus is a gram-negative bacterium that causes septicaemia and wound infection. cases occur sporadically, and no previous outbreaks due to a common source or a clonal strain have been reported. in the summer and autumn of 1996 and 1997, an outbreak of invasive v. vulnificus infection occurred in israel in people who had recently handled fresh, whole fish purchased from artificial fish-ponds. | 1999 | 10543668 |
| primary vibrio vulnificus bacteremia in a liver transplant recipient after ingestion of raw oysters: caveat emptor. | vibrio vulnificus is responsible for severe infections in chronically ill patients. organ transplant recipients are also at risk for severe infections due to v vulnificus. we report here the first case of v. vulnificus primary bacteremia due to raw shellfish consumption in a liver transplant recipient. all transplant patients should be cautioned against consuming uncooked seafood and warned about the risk of severe vibrio infections from seemingly innocuous wounds acquired in a salt water enviro ... | 1999 | 10532553 |
| in situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization. | bacterial communities in water samples and eel slime were investigated by fluorescence in situ hybridization of whole bacterial cells in an eel intensive culture system over 1 year. a newly developed probe, matching 27 vibrio spp., and a specific probe for vibrio vulnificus were used. phylogenetic probes complementary to selected regions of the 16s and 23s ribosomal rna revealed that proteobacteria of the alpha and beta subclass were predominant in water and eel slime. members of the gamma subcl ... | 1999 | 10530037 |
| modified dakin's solution for cutaneous vibrio infections. | vibrio species, specifically vibrio vulnificus, are known to be endemic to warm saltwater environments. as a human pathogen they are capable of causing severe, progressive, necrotizing infections. the lesions are bullous in nature and often require wide surgical debridement due to the aggressiveness of this organism. the literature supports prophylactic antibiotic therapy for those with preexisting hepatic dysfunction or immunocompromise. the authors routinely implement prophylactic antibiotic c ... | 1999 | 10517465 |
| perkinsus marinus extracellular protease modulates survival of vibrio vulnificus in eastern oyster (crassostrea virginica) hemocytes. | the in vitro effects of the perkinsus marinus serine protease on the intracellular survival of vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. results showed that protease-treated hemocytes were initially slower to internalize v. vulnificus than untreated hemocytes. after 1 h, the elimination of v. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. our d ... | 1999 | 10473449 |
| aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis. | a 49-year-old man, who had a 3-year history of liver dysfunction but had not been treated, was admitted to the hospital with a sudden onset of fever and generalized muscle pain. he subsequently developed generalized purpura with scattered hemorrhagic bullae of the skin and massive bloody stools. aeromonas sobria was proven by culture of both blood and bullous fluid. in spite of the extensive treatment with antibiotics and other medications in the intensive care unit (icu), the patient went into ... | 1999 | 10469397 |
| evaluation of the polymerase chain reaction method for identification of vibrio vulnificus isolated from marine environments. | the polymerase chain reaction (pcr) method for identification of vibrio vulnificus in the marine environment was evaluated by comparing it to both the conventional and dna-dna hybridization methods. of 13,325 isolates obtained from seawater and sediment samples, and oyster and goby specimens collected from the coastal waters of tokyo bay, japan, only 61 isolates were identified as v. vulnificus on the basis of phenotypic characteristics and the amplification of the cytotoxin-hemolysin gene by th ... | 1997 | 10465047 |
| vibrio vulnificus septicaemia and necrotizing fasciitis after a prick from the dorsal fin of a tilapia. | 1999 | 10450443 | |
| compartment syndrome of the forearm as the initial symptom of systemic vibrio vulnificus infection. | sepsis, cellulitis, and necrotizing fasciitis rarely have been described as causes of compartment syndrome. we report a case in which forearm compartment syndrome presented as the initial symptom of systemic infection. vibrio vulnificus, the etiologic pathogen of the compartment syndrome, was isolated from wound and blood cultures. the patient was treated with systemic antibiotic treatment and multiple forearm fasciotomies. the infectious process progressed rapidly, however and due to underlying ... | 1999 | 10447162 |
| seafood-associated disease outbreaks in new york, 1980-1994. | seafood-associated disease outbreaks in new york were examined to describe their epidemiology and to identify areas for prevention and control efforts. | 1999 | 10429753 |
| isolation of vibrio vulnificus from sea water and sand along the dan region coast of the mediterranean. | the occurrence of vibrio vulnificus incoastal sea water and sand was investigated. samples (286 in toto) were taken during the period between november 1993 and july 1994. ten v. vulnificus isolates (6.9%) were recovered from sea water and two isolates were recovered from sand (1.4%). the total isolation rate for this period was 4.2%. in a longer period of investigation, from june 1996 until june 1998 (24 months), 1,248 samples were taken and 205 v. vulnificus isolates were recorded (32.8%) in se ... | 1999 | 10413865 |
| identification and initial characterization of elastase activity associated with vibrio cholerae. | strains of vibrio cholerae o1 (ogawa, inaba) and non-o1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. the elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a ph range between 7 and 8. comparative studies indicate that more than 60% of v. cholerae strains analyzed quantitatively produce more elastase in broth (two- to fourfold higher) than ... | 1999 | 10398830 |
| factors influencing in vitro killing of bacteria by hemocytes of the eastern oyster (crassostrea virginica). | a tetrazolium dye reduction assay was used to study factors governing the killing of bacteria by oyster hemocytes. in vitro tests were performed on bacterial strains by using hemocytes from oysters collected from the same location in winter and summer. vibrio parahaemolyticus strains, altered in motility or colonial morphology (opaque and translucent), and listeria monocytogenes mutants lacking catalase, superoxide dismutase, hemolysin, and phospholipase activities were examined in winter and su ... | 1999 | 10388697 |
| use of mutant strain for evaluating processing strategies to inactivate vibrio vulnificus in oysters. | vibrio vulnificus is a ubiquitous marine bacterium frequently isolated from shellfish and associated with severe and often fatal disease in humans. various control strategies to reduce the disease risk associated with v. vulnificus contamination in shellfish have been proposed. however, evaluating the efficacy of these control strategies is complicated because of the difficulty in distinguishing v. vulnificus from the high levels of background environmental vibrio spp. the purpose of this resear ... | 1999 | 10382646 |
| the use of a modified dakin's solution (sodium hypochlorite) in the treatment of vibrio vulnificus infection. | we report the first clinical use of a modified dakin's solution (0.025% sodium hypochlorite [naocl]) to halt the progress of severe cutaneous vibrio vulnificus infection in a critically ill patient. the regimen used arose from an initial in vitro study designed to examine the sensitivity of vibrio species to topical antimicrobial agents. twenty-eight wound isolates were tested against the following eight topical preparations: silver sulfadiazine (silvadene), nitrofurazone, mupirocin ointment (ba ... | 1999 | 10347673 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| pathogenesis of vibrio vulnificus. | this review describes the factors which are currently recognized as being central to the virulence of the human pathogen, vibrio vulnificus. this estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons who are susceptible to this bacterium. the organism is also able to produce life-threatening wound infections. we describe here the nature of both the wound and primary septicemia ... | 1999 | 10339810 |
| indole-positive vibrio vulnificus isolated from disease outbreaks on a danish eel farm. | vibrio vulnificus was isolated in 1996 from 2 disease outbreaks on a danish eel farm which used brackish water. a characteristic clinical sign was extensive, deep muscle necrosis in the head region. v. vulnificus was isolated from kidney, mucus, spleen, gill and intestine of diseased eels. thirty-two isolates were examined phenotypically and serologically for pathogenicity to eels and for correlation to ribotype and plasmid profile. biochemically, the isolates showed properties similar to those ... | 1999 | 10228875 |
| differential expression of vibrio vulnificus capsular polysaccharide. | vibrio vulnificus is a human pathogen whose virulence has been associated with the expression of capsular polysaccharide (cps). multiple cps types have been described; however, virulence does not appear to correlate with a particular cps composition. reversible-phase variation for opaque and translucent colony morphologies is characterized by changes in cps expression, as suggested by electron microscopy of cells stained nonspecifically with ruthenium red. isolates with opaque colony morphologie ... | 1999 | 10225881 |
| [vibrio vulnificus infection: clinical and bacteriological analysis of four cases]. | vibrio vulnificus (v. vulnificus) infection has recently been drawing attention as a high mortality disease especially in a patient with the preexisting chronic liver disease. the illness caused by v. vulnificus is divided into three groups such as primary septicemia, wound infection and gastrointestinal illness. primary septicemia, which is the most common in japan, is defined as a systemic illness presenting fever or hypotension with recovery of v. vulnificus from blood or tissue without the a ... | 1999 | 10213992 |
| ferric-reductase activities in vibrio vulnificus biotypes 1 and 2. | in this paper, the ferric-reductase activities of vibrio vulnificus were investigated. this species comprises two biotypes pathogenic for humans and eels that are able to express different mechanisms for iron acquisition. all strains of both biotypes used in this study were able to reduce ferric citrate, irrespective of the iron levels in the growth medium. some variation in the degree of reduction was observed among the strains, with the highest values corresponding to one acapsulated environme ... | 1999 | 10188249 |
| the hemagglutinating action of vibrio vulnificus metalloprotease. | vibrio vulnificus protease (vvp), a 45-kda zinc metalloprotease, consists of two functional domains: an n-terminal 35-kda polypeptide having endoproteinase activity, and a c-terminal 10-kda polypeptide that mediates the binding of vvp to the erythrocyte membrane. therefore, vvp, but not its n-terminal endoproteinase domain alone, has agglutinating activity to rabbit erythrocytes. when a single zinc atom in the catalytic center was substituted by treatment with cucl2 or nicl2, proteolytic and hem ... | 1999 | 10100751 |
| skin and soft-tissue infections after injury in the ocean: culture methods and antibiotic therapy for marine bacteria. | isolated organisms from two common indo-pacific marine animals (echinometra mathaei urchins and acanthaster planci sea stars) likely to cause puncture wounds to recreational beachcombers, diverse, or operational military forces during amphibious assaults demonstrate why practitioners should consider their first choice for potential antibiotic therapy differently from their usual favorite antibiotics. the effects of thiosulfate-citrate-bile-sucrose (tcbs) agar, varying salt concentrations in the ... | 1999 | 10091493 |
| [vibrio vulnificus septicemia in spain]. | vibrio vulnificus is a virulent marine organism, able to contaminate sea-food. it usually produces bacteremia associated with secondary skin lesions in patients with underlying conditions, such as hepatic cirrhosis. we report a case of septic shock and characteristic skin lesions, due to vibrio vulnificus in a patient with cirrhosis, who had eaten raw oysters. the patient survived in spite of the severity of the clinical picture. we conclude that vibrio vulnificus infection must be considered in ... | 1998 | 10079541 |
| the ability of vibrio vulnificus to use a synthetic hydrophilic heme compound, fe-tpps, as a single iron source. | vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. this process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin ix). in the present study, we tested whether this pathogen also uses a synthetic heme compound, fe-alpha,beta,gamma,delta-tetraphenylporphine tetrasulfonic acid (fe-tpps), as an iron source. when inoculated into a medium containing fe-tpps, v. vulnificus l-180 multiplication was seen ... | 1999 | 10079530 |
| isolation of vibrio vulnificus serovar e from aquatic habitats in taiwan. | the existence of strains of vibrio vulnificus serovar e that are avirulent for eels is reported in this work. these isolates were recovered from water and oysters and differed from eel virulent strains in (i) fermentation and utilization of mannitol, (ii) ribotyping after hindiii digestion, and (iii) susceptibility to eel serum. lipopolysaccharide of these strains lacked the highest molecular weight immunoreactive bands, which are probably involved in serum resistance. | 1999 | 10049908 |
| randomly amplified polymorphic dna analysis of clinical and environmental isolates of vibrio vulnificus and other vibrio species. | vibrio vulnificus is an estuarine bacterium that is capable of causing a rapidly fatal infection in humans. a randomly amplified polymorphic dna (rapd) pcr protocol was developed for use in detecting v. vulnificus, as well as other members of the genus vibrio. the resulting rapd profiles were analyzed by using rflpscan software. this rapd method clearly differentiated between members of the genus vibrio and between isolates of v. vulnificus. each v. vulnificus strain produced a unique band patte ... | 1999 | 10049874 |
| effects of salinity and temperature on long-term survival of the eel pathogen vibrio vulnificus biotype 2 (serovar e). | vibrio vulnificus biotype 2 (serovar e) is a primary eel pathogen. in this study, we performed long-term survival experiments to investigate whether the aquatic ecosystem can be a reservoir for this bacterium. we have used microcosms containing water of different salinities (ranging from 0.3 to 3.8%) maintained at three temperatures (12, 25, and 30 degrees c). temperature and salinity significantly affected long-term survival: (i) the optimal salinity for survival was 1.5%; (ii) lower salinities ... | 1999 | 10049871 |
| cytotoxicity of vibrio vulnificus cytolysin on rat peritoneal mast cells. | histamine has been thought to be a permeability enhancing factor in vibrio vulnificus infection. the injection of living bacteria or purified v. vulnificus cytolysin (vvc) can cause lethality in mice by inducing hemoconcentration and increased vascular permeability. in the present study, we tried to identify whether histamine release causes the increased vascular permeability that is responsible for the lethal effect of vvc. treatment of rat peritoneal mast cells with high concentrations of vvc ... | 1998 | 10037218 |
| low incidence of vibrio vulnificus among vibrio isolates from sea water and shellfish of the western mediterranean coast. | a specific search for vibrio vulnificus in natural marine samples from the spanish mediterranean sea was carried out by nested pcr and cultural approaches using thiosulphate-citrate-bile salts-sucrose agar (tcbs) and cellobiose-polymixin b-colistin agar (cpc), incubated at 40 degrees c, as selective media. presumptive colonies were identified by pcr using specific primers against 23s rrna sequences. this species was isolated from sea water and edible bivalves, mainly after preenrichment in alkal ... | 1999 | 10030016 |
| activation of particulate guanylyl cyclase by vibrio vulnificus hemolysin. | recently we reported that vibrio vulnificus hemolysin, an exotoxin produced by v. vulnificus, dilates rat thoracic aorta via elevated cgmp levels without affecting nitric oxide synthase. we investigated the mechanism further by observing the guanylyl cyclase activities in cytosolic, membrane, unfractionated, or reconstituted preparations. hemolysin did not activate guanylyl cyclase in the membrane or cytosolic fraction, while it activated guanylyl cyclase in unfractionated or reconstituted prepa ... | 1999 | 9988111 |
| comparative study of biological properties and electrophoretic characteristics of lipopolysaccharide from eel-virulent and eel-a virulent vibrio vulnificus strains. | in vibrio vulnificus, virulence for eels is associated with serovar e strains. in this study, we investigated some biological properties of purified lipopolysaccharides (lpss) from serovar e and non-serovar e strains. purified lpss retained their o-polysaccharidic side chains and did not show any differences that could be related to host specificity, except for serological differences. | 1999 | 9925630 |
| adaptive response to cold temperatures in vibrio vulnificus. | the effectiveness of rapid chilling or freezing of oysters to reduce vibrio vulnificus levels in shellfish may be compromised by product handling procedures that permit cold adaptation. when a v. vulnificus culture was shifted from 35 degrees c to 6 degrees c conditions, it underwent transition to a non-culturable state. cells adapted to 15 degrees c prior to change to 6 degrees c condition, however, remain viable and culturable. in addition, cultures adapted to 15 degrees c were able to survive ... | 1999 | 9922468 |
| hand infections due to non-cholera vibrio after injuries from st peter's fish (tilapia zillii). | we report 49 patients with a wide variety of hand infections, which developed after injuries from st peter's fish (tilapia zillii). twenty-eight of 36 patients who had been operated on had non-cholera vibrio infections, all identified as vibrio vulnificus. the course in these patients was characterized by rapid spread of the infection with progressive necrosis of the tendon sheath, subcutaneous tissues and the skin. two of them required amputations but the others had satisfactory functional resu ... | 1998 | 9888689 |
| necrotizing fasciitis caused by vibrio vulnificus: first published infection acquired in turkey is the second time a strain is isolated in germany. | vibrio vulnificus, a marine vibrio, has recently been recognized as a potential human pathogen. it causes human infections with mortality rates up to 60%. until 1991, most human isolations were reported from the usa, japan and taiwan. the second strain isolated in germany is documented and a significant case of v. vulnificus infection acquired in turkey is published for the first time. | 1998 | 9861568 |
| survival of vibrio vulnificus in whole blood from patients with chronic liver diseases: association with phagocytosis by neutrophils and serum ferritin levels. | vibrio vulnificus causes severe wound infections and sepsis, mostly in persons with chronic liver diseases. survival of this organism in the whole blood collected from healthy volunteers and patients with chronic hepatitis, liver cirrhosis, and hepatoma was analyzed as an indication of susceptibility. the bacterial numbers in the blood after 5 h of incubation tended to increase with the severity of the liver disease and differed significantly between hepatoma patients and healthy volunteers (p<. ... | 1999 | 9841854 |
| heterogeneity among isolates of vibrio vulnificus recovered from eels (anguilla anguilla) in denmark. | the findings of this study demonstrate that vibrio vulnificus isolates recovered from diseased eels in denmark are heterogeneous as shown by o serovars, capsule types, ribotyping, phage typing, and plasmid profiling. the study includes 85 v. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two danish eel farms from 1995 to 1997, along with a collection of 12 v. vulnificus reference strains. the results showed th ... | 1998 | 9835548 |
| the type iv leader peptidase/n-methyltransferase of vibrio vulnificus controls factors required for adherence to hep-2 cells and virulence in iron-overloaded mice. | vibrio vulnificus expresses a number of potential virulence determinants that may contribute to its ability to cause a severe and rapidly disseminating septicemia in susceptible hosts. we have cloned and characterized two genes encoding products related to components of the type iv pilus biogenesis and general secretory (type ii) pathways by complementation of a type iv peptidase/n-methyltransferase (pild) mutant of pseudomonas aeruginosa with a v. vulnificus genomic library. one of the genes (v ... | 1998 | 9826339 |
| infections due to non-o1 vibrio cholerae in southern taiwan: predominance in cirrhotic patients. | although taiwan is not an area where cholera is endemic, from october 1988 to october 1997 30 episodes of non-o1, non-o139 vibrio cholerae infection were noted at the national cheng kung university hospital in taiwan. infections generally occurred in hot seasons, and two episodes were concomitant with vibrio vulnificus infection. three major clinical presentations were found: bacteremia with concurrent spontaneous bacterial peritonitis or invasive soft-tissue infections that occurred solely in c ... | 1998 | 9798033 |
| raw shellfish consumption among renal disease patients. a risk factor for severe vibrio vulnificus infection. | raw shellfish-associated vibrio vulnificus septicemia, with a case-fatality rate of nearly 50%, occurs most commonly in immunocompromised patients or those with liver disease. | 1998 | 9791643 |
| identification of the vibrio cholerae type 4 prepilin peptidase required for cholera toxin secretion and pilus formation. | cholera toxin secretion is dependent upon the extracellular protein secretion apparatus encoded by the eps gene locus of vibrio cholerae. although the eps gene locus encodes several type four prepilin-like proteins, the peptidase responsible for processing these proteins has not been identified. this report describes the identification of a prepilin peptidase from the v. cholerae genomic database by virtue of its homology with the pild prepilin peptidase of pseudomonas aeruginosa. plasmid disrup ... | 1998 | 9781884 |
| ribotyping of clinical vibrio vulnificus isolates. | restriction fragment length polymorphism analysis of rrna genes (ribotyping) was used to differentiate vibrio vulnificus isolates. among the 10 restriction enzymes tested, hindiii was shown to provide the most discriminatory patterns. stul was used for further analysis of strains that were indistinguishable with hindiii. thirteen clinical v. vulnificus strains were analyzed for their ribotypes with hindiii, as well as stul when necessary. four of the clinical strains were isolated from different ... | 1995 | 9775004 |
| differentiation of vibrio vulnificus strains by an arbitrarily primed polymerase chain reaction. | a synthetic 17 mer oligonucleotide (5'-gttgggtaacgccaggg-3') was used as a primer for the arbitrarily primed polymerase chain reaction (ap-pcr) to differentiate various strains of vibrio vulnificus. a total of 37 genomic dnas that were extracted from the clinical and environmental strains were successfully differentiated. among them, 32 profiles of the 37 strains were characterized. none of the environmental and clinical strains had the same amplification profile, suggesting the highly heterogen ... | 1995 | 9774986 |
| the use of alkaline phosphatase-labelled oligonucleotide probes as culture confirmation reagents for identification of commercially important bacteria. | a range of rrna-targeted alkaline phosphatase labelled oligonucleotide probes was tested for use as culture confirmation reagents for the rapid identification of micro-organisms. the probes were specific to clinically important bacteria (helicobacter pylori and mycobacterium tuberculosis), fish and shellfish pathogens (renibacterium salmoninarum and vibrio vulnificus), food spoilage bacteria (listeria spp. and l. monocytogenes), for bacteria of biotechnological importance (streptomyces spp.) and ... | 1998 | 9750334 |
| characterization of the hemorrhagic reaction caused by vibrio vulnificus metalloprotease, a member of the thermolysin family. | vibrio vulnificus is an opportunistic human pathogen causing wound infections and septicemia, characterized by hemorrhagic and edematous damage to the skin. this human pathogen secretes a metalloprotease (v. vulnificus protease [vvp]) as an important virulence determinant. when several bacterial metalloproteases including vvp were injected intradermally into dorsal skin, vvp showed the greatest hemorrhagic activity. the level of the in vivo hemorrhagic activity of the bacterial metalloproteases ... | 1998 | 9746589 |
| a comparison of strategies for the detection and recovery of vibrio vulnificus from marine samples of the western mediterranean coast. | we have compared the effectiveness of culture-based methods and a dna-based method for the detection, of vibrio vulnificus from a seawater and three types of shellfish collected from the costal waters of valencia, spain. for culture-based method, we used two selective media, thiosulphate-citrate-salts-sucrose (tcbs), and cellobiose-polymyxin b-colistin (cpc) agars with and without previous enrichment in alkaline-saline-peptone-water (apws). presumptive colonies were confirmed as v. vulnificus by ... | 1998 | 9741117 |
| direct identification of vibrio vulnificus in clinical specimens by nested pcr. | this study was performed to establish optimal nested pcr conditions and a high-yield dna extraction method for the direct identification of vibrio vulnificus in clinical specimens. we designed two sets of primers targeting the v. vulnificus hemolysin/cytolysin gene. the target of the first primer set (p1-p2; sense, 5'-gac-tat-cgc-atc-aac-aac-cg-3', and antisense, 5'-agg-tag-cga-gta-tta-ctg-cc-3', respectively) is a 704-bp dna fragment. the second set (p3-p4; sense, 5'-gct-att-tca-ccg-ccg-ctc-ac- ... | 1998 | 9738039 |
| the role of gulf coast oysters harvested in warmer months in vibrio vulnificus infections in the united states, 1988-1996. vibrio working group. | vibrio vulnificus infections are highly lethal and associated with consumption of raw shellfish and exposure of wounds to seawater. v. vulnificus infections were reported to the centers for disease control and prevention from 23 states. for primary septicemia infections, oyster trace-backs were performed and water temperature data obtained at harvesting sites. between 1988 and 1996, 422 infections were reported; 45% were wound infections, 43% primary septicemia, 5% gastroenteritis, and 7% from u ... | 1998 | 9728544 |
| genetic relatedness among environmental, clinical, and diseased-eel vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic dna pcr. | genetic relationships among 132 strains of vibrio vulnificus (clinical, environmental, and diseased-eel isolates from different geographic origins, as well as seawater and shellfish isolates from the western mediterranean coast, including reference strains) were analyzed by random amplified polymorphic dna (rapd) pcr. results were validated by ribotyping. for ribotyping, dnas were digested with kpni and hybridized with an oligonucleotide probe complementary to a highly conserved sequence in the ... | 1998 | 9726889 |
| minimum inhibitory concentration of smoke wood extracts against spoilage and pathogenic micro-organisms associated with foods. | antimicrobial activity of seven commercial smoke preparations (four liquid and three solid) was studied. the minimum inhibitory concentration (mic) was determined against a selection of food spoilage and pathogenic micro-organisms. the main smoke components were identified and quantified by gas chromatography/mass spectrometry. the most effective condensate was s2. all strains except salmonella enteritidis were inhibited by s2 with an mic < 0.5-1.5%. smoke extract l2 inhibited growth of vibrio v ... | 1998 | 9722997 |
| structure of a muramic acid containing capsular polysaccharide from the pathogenic strain of vibrio vulnificus atcc 27562. | vibrio vulnificus strains isolated from septicemia cases and from the environment show a wide variety of capsular types. in an attempt to find common structural features which can be correlated with pathogenicity and toxicity, we have determined structures of the capsular polysaccharides (cps) from several pathogenic strains. we report the complete structure of the polysaccharide from the pathogenic v. vulnificus strain atcc 27562 using a combination of homonuclear and heteronuclear one-dimensio ... | 1998 | 9720237 |
| characterization of vibrio vulnificus isolated from cockles (anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic dna analysis. | antibiotic susceptibility, plasmid profiles and random amplification of polymorphic dna (rapd) were used to study strains of vibrio vulnificus isolated from cockles (anadara granosa). thirty-six isolates were analyzed. the prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid dna bands ranging in size from 1.4 to 9.7 mda. thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial ... | 1998 | 9711850 |
| endotoxin removal column containing polymyxin b immobilized fiber is useful for the treatment of the patient with vibrio vulnificus septicemia. | a case of primary septicemia due to vibrio vulnificus infection is reported. the patient was successfully treated with appropriate antibiotic therapy, drainage, and debridement of the necrotic tissues and direct hemoperfusion (dhp) using polymyxin b immobilized fiber (pmx-f). the effectiveness of dhp using pmx-f, which removes endotoxin in the circulating blood for the treatment of septic shock and multiple organ dysfunction occurring due to this fulminant infectious disease, is discussed. | 1998 | 9702324 |
| structure determination of the capsular polysaccharide from vibrio vulnificus strain 6353. | vibrio vulnificus is a pathogenic gram-negative bacterium, endemic to brackish waters, which is often isolated from sediments, from the water column or from shellfish. it is associated with wound infections and septicemia in humans and the virulence of v. vulnificus has been strongly associated with encapsulation. the capsular polysaccharide purified from a virulent strain of v. vulnificus 6353 did not show cross reactivity with antibodies to the capsular polysaccharide of a related pathogenic s ... | 1998 | 9692929 |
| randomly amplified polymorphic dna analysis of starved and viable but nonculturable vibrio vulnificus cells. | vibrio vulnificus is an estuarine bacterium capable of causing a rapidly fatal infection in humans. because of the low nutrient levels and temperature fluctuations found in the organism's natural habitat, the starvation state and viable but nonculturable (vbnc) state are of particular interest. a randomly amplified polymorphic dna (rapd) pcr protocol was developed previously for the detection of v. vulnificus strains grown in rich media and has been applied to starved and vbnc cells of v. vulnif ... | 1998 | 9687468 |
| virulence factors and pathogenicity of vibrio vulnificus strains isolated from seafood. | the virulence factors of vibrio vulnificus are not yet well understood. so far, many hydrolytic enzymes have been implicated in the pathogenesis of this micro-organism. the present research was carried out in order to study the presence of some of these enzymes in 133 v. vulnificus strains isolated from 45 seafood samples. the results showed that 100% of these strains were positive for the production of lecithinase and lipase (tween-80), 99.2% for caseinolytic protease, 96.9% for dnase, 65.4% fo ... | 1998 | 9674127 |
| effects of extracellular products of vibrio vulnificus on acanthopagrus schlegeli serum components in vitro and in vivo. | a vibrio strain ls001, originally isolated from a body surface lesion of a moribund black porgy (acanthopagrus schlegeli) in 1994 in taiwan, was identified as vibrio vulnificus. the extracellular products (ecp) of the strain were lethal to the fish, and its effects on fish serum in vitro and in vivo are described in the present study. nine major precipitation arcs were visualized in normal fish serum in a crossed immunoelectrophoresis (cie) gel using rabbit antiserum to the fish normal serum and ... | 1997 | 9670552 |
| detection of microbial pathogens in shellfish with multiplex pcr. | multiplex pcr amplification of uida, cth, inva, ctx, and tl genes was developed enabling simultaneous detection in shellfish of escherichia coli, an indicator of fecal contamination and microbial pathogens, salmonella typhimurium, vibrio vulnificus, v. cholerae, and v. parahaemolyticus, respectively. each of the five pairs of oligonucleotide primers was found to support pcr amplifications of only its targeted gene. the optimized multiplex pcr reaction utilized a pcr reaction buffer containing 2. ... | 1998 | 9662609 |
| vibrio vulnificus sepsis associated with coincidental diagnosis of acute myeloid leukemia. | 1998 | 9662287 | |
| necrotizing fasciitis caused by vibrio vulnificus differs from that caused by streptococcal infection. | we reviewed the clinical record of all patients admitted to saga medical school hospital during the most recent 10 years and found that 17 (0.03%) were diagnosed as having necrotizing fasciitis. bacteriological examination demonstrated that vibrio vulnificus was the pathogen responsible in five patients (29%). the disease caused by v. vulnificus occurred in the warmer half of the year. all of the patients had underlying chronic liver dysfunction, and three of them had previously consumed raw sea ... | 1998 | 9661943 |
| [vibrio vulnificus infections]. | 1998 | 9648448 | |
| enhanced broth media for selective growth of vibrio vulnificus. | rapid detection of vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. pnc (5% peptone, 1% nacl, and 0.08% cellobiose [ph 8.0]) enhanced the growth of v. vulnificus compared to alkaline peptone broth. pncc (pnc with 1.0 to 4.1 u of colistin methanesulfonate per ml) increased the growth of low levels of v. vulnificus while suppressing non-target bacteria. | 1998 | 9647852 |
| cloning and characterization of an outer membrane protein of vibrio vulnificus required for heme utilization: regulation of expression and determination of the gene sequence. | vibrio vulnificus is a halophilic, marine pathogen that has been associated with septicemia and serious wound infections in patients with iron overload and preexisting liver disease. for v. vulnificus, the ability to acquire iron from the host has been shown to correlate with virulence. v. vulnificus is able to use host iron sources such as hemoglobin and heme. we previously constructed a fur mutant of v. vulnificus which constitutively expresses at least two iron-regulated outer membrane protei ... | 1998 | 9632577 |
| vibrio vulnificus septicemia in a patient with severe aplastic anemia. | a 53-year-old man with severe aplastic anemia developed sporadic vibrio vulnificus septicemia 1 day after eating raw fish and shellfish. although v. vulnificus infection is potentially fatal, he was saved by immediate and sensitive antibiotic administration. patients with chronic hematologic disease are susceptible to infection by this organism and are prone to developing septicemia when they eat raw seafood. it is necessary for a patient with this infection to be given effective antibiotics as ... | 1998 | 9631584 |
| [recovery from septicemia caused by fecally identified vibrio vulnificus and complicated by ulcerative colitis ]. | 1998 | 9627485 | |
| minocycline and cefotaxime in the treatment of experimental murine vibrio vulnificus infection. | we conducted an in vivo study with the mouse model of vibrio vulnificus infection to evaluate the efficacies of therapy with minocycline or cefotaxime alone and in combination. v. vulnificus was introduced subcutaneously into the area over the right thigh. the inoculum size ranged from 1.0 x 10(3) to 1.2 x 10(8) cfu from experiment to experiment but was constant for all animals in the same experiment. antibiotics were given intraperitoneally 2 h after the bacteria were inoculated. in experiments ... | 1998 | 9624467 |
| an epimerase gene essential for capsule synthesis in vibrio vulnificus. | the extracellular capsule polysaccharide (cps) of vibrio vulnificus is a primary virulence factor which allows survival of the bacteria in the human host. to study the genes involved in expression of the capsule, we generated mutants that lost the ability to produce cps following the insertion of a minitransposon into the genome of an encapsulated, clinical strain of v. vulnificus. a genomic region, from one nonencapsulated mutant, containing the transposon and flanking v. vulnificus dna was clo ... | 1998 | 9596722 |
| improved isolation of vibrio vulnificus from seawater and sediment with cellobiose-colistin agar. | an improved selective medium, cellobiose-colistin (cc) agar, gave a significantly higher (p < 0.05) isolation rate of vibrio vulnificus from water and sediment samples than did modified cellobiose-polymyxin b-colistin (mcpc) agar. in a total of 446 alkaline peptone water preenrichments amended with polymyxin b, v. vulnificus was isolated from 154 preenrichments (35%) with mcpc agar and from 179 preenrichments (40%) with cc agar. cc agar gave a higher plating efficiency of v. vulnificus cells tha ... | 1998 | 9572942 |
| occurrence and distribution of vibrio vulnificus in tropical fish and shellfish from cochin (india). | the incidence and distribution of vibrio vulnificus in marine and brackish-water fish and shellfish from coastal areas of cochin on the west coast of india were studied. for marine fish collected in very fresh condition from vessels, a level of incidence of 16.6% was noted. frequency of isolation was greater from the intestine than from the muscle. the greater most probable number (mpn) count determined by the three tube method ranged from 15 to 910 g-1 in the positive samples. | 1998 | 9569691 |
| molecular typing of vibrio vulnificus isolates by random amplified polymorphic dna (rapd) analysis. | this study was undertaken to determine molecular types and genetic similarity among v. vulnificus isolates by rapd analysis. we compared these results with serotypes of v. vulnificus. ninety-seven v. vulnificus strains including 69 strains from chonnam university hospital (cuh; kwangju, korea), 13 from wonkwang university hospital (wuh; iksan, korea), 13 from the japanese national institute of health (jnih) and two reference strains (atcc 33815 and atcc 27562) were analyzed. four molecular types ... | 1997 | 9556752 |
| influence of water temperature and salinity on vibrio vulnificus in northern gulf and atlantic coast oysters (crassostrea virginica). | this study investigated the temperature and salinity parameters associated with waters and oysters linked to food-borne vibrio vulnificus infections. v. vulnificus was enumerated in oysters collected at three northern gulf coast sites and two atlantic coast sites from july 1994 through september 1995. two of these sites, black bay, la., and apalachicola bay, fla., are the source of the majority of the oysters implicated in v. vulnificus cases. oysters in all gulf coast sites exhibited a similar ... | 1998 | 9546182 |
| activity of bacterial phosphomonoesterases in batch culture. | the phosphomonoesterases catalyse the hydrolysis of primary esters of phosphoric acid which help the bacteria to survive in phosphate stressed environment. ninety-five bacterial isolates were obtained from domestic sewage and industrial effluents of gelatine and soap factories at jabalpur on a medium enriched with phosphate and were screened for phosphatase production. the phosphatase producers were tentatively identified as escherichia coli, vibrio vulnificus, aeromonas hydrophila, staphylococc ... | 1998 | 9536656 |
| isolation of a hemin and hemoglobin binding outer membrane protein of vibrio vulnificus biotype 2 (serogroup e). | the eel pathogen vibrio vulnificus biotype 2 (serogroup e) is able to use hemin (hm) or hemoglobin (hb) as the sole iron source for growth in vitro and in vivo. the mechanism of heme-iron acquisition in this bacterium requires a direct interaction through binding sites on the bacterial surface (constitutive outer membrane proteins). using affinity chromatography techniques, a unique protein of around 36.5 kda was isolated from cell envelopes of e86 strain regardless of the affinity ligand used, ... | 1997 | 9513263 |
| fatal sepsis from vibrio vulnificus in a hemodialyzed patient. | vibrio vulnificus, a particularly virulent halophilic vibrio, has been isolated from the blood and skin necrotic lesion of a hemodialyzed patient with sepsis. the patient has had exposure of the skin to seawater. various chronic conditions including renal failure have a great risk for developing septicemia due to v vulnificus. it is necessary to inform persons with liver diseases or immunocompromising conditions of hazards associated with the consumption of undercooked seafood and seawater expos ... | 1998 | 9496743 |
| vibrio vulnificus infections: case reports and literature review. | vibrio vulnificus is an emerging opportunistic pathogen that is responsible for one of the most fulminant food-borne diseases in immunocompromised hosts. the infection should be suspected in patients with underlying liver disorders who present with a prominent skin rash, hypotension and septic parameters especially if they give a history of seafood or shellfish ingestion. awareness and early recognition of this infection will result in a better outcome for the patient. | 1997 | 9494683 |
| clinical infections of vibrio vulnificus: a case report and review of the literature. | vibrio vulnificus is a marine gram-negative bacillus that is recognized as a cause of fulminant primary septicemia and wound infections. one of the most common bacteria in seawater, v. vulnificus is concentrated in ocean filter feeders (e.g., oysters and clams). primary septicemia can occur in patients, typically with underlying liver disease, who have acquired the organism through the gastrointestinal tract after recent consumption of raw shellfish. characterized by fevers, chills, and bullous ... | 1998 | 9472762 |