Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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antibody-dependent cell-mediated cytotoxicity against ibr-infected bovine kidney cells by ruminant neutrophils: the role of lysosomal cationic protein. | antibody-dependent cell-mediated cytotoxicity (adcc) of infectious bovine rhinotracheitis (ibr)-infected bovine kidney cells (mdbk) by neutrophils was demonstrated. neutrophils from bovine and sheep mammary exudate and peripheral blood, and also from human peripheral blood, were all active in the presence of anti-ibr antibody. the component of the ruminant neutrophil granules which was responsible for cytotoxicity appeared to be cationic protein since purified cationic protein lysed the virus-in ... | 1984 | 6092270 |
the possible role of stress in the induction of pneumonic pasteurellosis. | five groups of range bred calves (four calves per group) were used to investigate the effect of stress on susceptibility to aerosol exposures with bovine herpesvirus-1 or pasteurella haemolytica. twelve calves were weaned, transported, processed at a commercial feedlot and transported to isolation facilities three days later. an aerosol challenge of either 10 colony forming units of p. haemolytica or 10 plaque forming units of bovine herpesvirus-1 virus was given to two groups of calves and the ... | 1984 | 6089981 |
prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec. | serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ... | 1984 | 6089626 |
serologic cross-reaction between bovine herpesviruses 1 and 4 by the enzyme-linked immunosorbent assay. | reciprocal cross-titrations using constant concentrations of bovine antiserum (1: 100) to either bovine herpesvirus (bhv) 1 or bhv 4 (obtained from controlled infection status) against varying concentrations of both viral antigens in enzyme-linked immunosorbent assay (elisa) indicated a subtle difference between the antigenic composition of these 2 agents. although control antiserum consistently yielded higher elisa titers against homologous viral antigen, a considerable cross-reactivity between ... | 1984 | 6087088 |
comparative studies of strains of infectious bovine rhinotracheitis virus isolated from latently infected calves. | three strains (479 c, 778 tl, 982 le) of infectious bovine rhinotracheitis (ibr) virus isolated from latently infected calves were compared with the prototype strain of ibr virus (la strain) in studies which included restriction endonuclease analysis, experimental infection, and reciprocal cross protection tests in cattle. from the restriction endonuclease analysis it appeared that the 3 "latent" viruses were derived from the same isolate, and that it differed slightly from the la strain. howeve ... | 1984 | 6086223 |
virus isolations from cases of infectious bovine pustlo-vulvovaginitis and posthitis (ipv/ipb) in cattle in tanzania. | 1974 | 4378003 | |
role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. | when cattle infected with infectious bovine rhinotracheitis virus (ibrv) were treated 3 months later with a synthetic corticosteroid, consistent recrudescence of the infection was observed. suppression of cell-mediated immunity, as measured by lymphocyte transformation responses, could be demonstrated at the time of recrudescence. treatment with adrenocorticotropic hormone and trigeminal neurotomy also resulted in recrudescence of ibrv, but immunosuppression was not detected in these animals. th ... | 1973 | 4355136 |
a micro-passive hemagglutination test for the rapid detection of antibodies to infectious bovine rhinotracheitis virus. | serum antibodies to infectious bovine rhinotracheitis virus are most commonly detected using the serum-virus-neutralization test, a time-consuming and expensive procedure. a passive hemagglutination test was developed for the rapid detection of antibodies using the microtiter system. two cloned strains of the virus were used in the investigation, namely the bf strain and the los angeles strain. erythrocytes from several species were used. the best results were obtained using the los angeles stra ... | 1971 | 4338673 |
respiratory viruses of cattle. | 1971 | 4335759 | |
probable occurrence of infectious bovine rhinotracheitis virus in tanzania in wildlife and cattle. | 1970 | 4312880 | |
[epizootiology and prevention, in the territory of the federal republic of germany, of virus diseases of cattle affecting the mucous membranes]. | 1966 | 4298152 | |
complications in cattle following vaccination with a combined bovine viral diarrhea-infectious bovine rhinotracheitis vaccine. | 1968 | 4297313 | |
viral respiratory infection in commercial feedlot cattle. | 1967 | 4291850 | |
isolation and characterization of a strain of infectious bovine rhinotracheitis virus associated with enteritis in cattle: pathogenesis studies by fluorescent antibody tracing. | 1966 | 4291328 | |
isolation and characterization of a strain of infectious bovine rhinotracheitis virus associated with enteritis in cattle: isolation, serologic characterization, and induction of the experimental disease. | 1966 | 4291326 | |
morphometric studies of the bovine uterus: microscopic lesions and retrospective reproductive history. | a survey of reproductive tracts from nonpregnant cows at an abattoir was conducted. reproductive tracts from 98 cows were studied microscopically and evaluated with a grading system to determine the severity of pathologic changes. inflammatory reaction (type and distribution), fibrosis, gland morphology, and appearance were measured, quantified, and scored (1 to 4). category 1 endometrium (normal) was found in 18 cows, category 2 in 23, category 3 in 34, and category 4 in 23. the category of end ... | 1985 | 4083598 |
parasites, diseases, and health status of sympatric populations of fallow deer and white-tailed deer in kentucky. | in august 1983, a study on parasites, diseases, and health status was conducted on sympatric populations of fallow deer (dama dama) and white-tailed deer (odocoileus virginianus) from land between the lakes, lyon and trigg counties, kentucky. five adult deer of each species were studied. white-tailed deer had antibodies to epizootic hemorrhagic disease (ehd) virus and leptospira interogans serovariety icterohemorrhagiae, and fallow deer had antibodies to bluetongue and ehd viruses. serologic tes ... | 1985 | 3999248 |
serum complement-fixing antibody to pasteurella haemolytica a1 and its effect on experimentally induced pneumonic pasteurellosis in calves. | ninety-three calves comprising 16 experimental groups were exposed to viral (bovine herpesvirus-1 or parainfluenza-3 virus) and pasteurella haemolytica aerosols. serum samples from these calves were tested before and after exposure for antibodies to p haemolytica by a modified direct complement-fixation test. at slaughter of the calves, the extent of pneumonia produced was estimated for each calf and compared with the results of the modified direct complement-fixation tests. the extent of pneumo ... | 1985 | 3909862 |
preliminary studies with a live streptomycin-dependent pasteurella multocida and pasteurella haemolytica vaccine for the prevention of bovine pneumonic pasteurellosis. | twelve pasteurella-free holstein-friesian calves were used in a study to test the efficacy of a live streptomycin-dependent pasteurella multocida a:3 and streptomycin-dependent pasteurella haemolytica a1 vaccine. the calves were inoculated intramuscularly twice at 14-day intervals with either the streptomycin-dependent vaccine, containing 1 x 10(6) colony forming units/ml p. multocida and 4 x 10(8) colony forming units/ml p. haemolytica, commercial bacterin, or phosphate buffered saline. two wee ... | 1985 | 3907804 |
clinical and experimental modifications of plasma iron and zinc concentrations in cattle. | plasma iron and zinc concentrations were studied in pyrexic cattle or in cattle experimentally infected with infectious bovine rhinotracheitis virus or escherichia coli endotoxin. plasma iron and zinc levels tended to decline in the animals given endotoxin and in the pyrexic cattle, but the plasma iron level was only modified after experimental infectious bovine rhinotracheitis infection. these changes were not always related to pyrexia. plasma iron and zinc concentrations taken together may be ... | 1985 | 3890352 |
serologic survey of viral antibodies in the peruvian alpaca (lama pacos). | sera from more than 100 alpacas (lama pacos) from the peruvian southern sierra were examined for antibodies to 8 viruses known to infect other domestic animals. on the basis of these serologic findings and previously published serologic or clinical data, it is now known that the alpaca can be infected with the following viruses: parainfluenza-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bluetongue virus, border disease virus, influenza a virus, rotavirus, rabies virus, vesicular ... | 1987 | 3826854 |
colonization of the nasal passages of calves with pasteurella haemolytica serotype 1 and regeneration of colonization after experimentally induced viral infection of the respiratory tract. | healthy nonstressed calves were inoculated intranasally with or subjected to aerosol exposure to pasteurella haemolytica serotype 1. only 4 of 28 calves harbored the bacterium in enough numbers to be isolated from the nasal passages for more than 7 days. after apparent clearing from the nasal passages, 8 calves were inoculated intranasally with infectious bovine rhinotracheitis virus; 2 of these calves shed the p haemolytica during clinical illness due to the virus. the remaining 20 calves were ... | 1986 | 3752678 |
study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
effect of dexamethasone on bovine leukocyte functions and bovine herpesvirus type-1 replication. | in an attempt to elucidate the mechanism whereby dexamethasone could reactivate bovine herpesvirus type-1 the effect of dexamethasone on virus replication and leukocyte functions was assessed. no effect was detectable on either virus yield or in vitro replication kinetics. in contrast, dexamethasone influenced several leukocyte functions thought to be of importance in antiviral defense and maintenance of latency. in vitro exposure of peripheral blood polymorphonuclear neutrophilic granulocytes o ... | 1987 | 3651888 |
effects of infectious bovine rhinotracheitis virus infection on bovine airway reactivity. | the response of isolated tracheal and bronchial strips to isoproterenol in vitro was studied in eleven male jersey calves. clinical, microbiological and pathological evaluations of the calves were carried out. in calves exposed once or twice to infectious bovine rhinotracheitis virus, the relaxation threshold of the trachealis muscle to isoproterenol was significantly (p less than 0.05) impaired (threshold 5.0 x 10(-7) m, single exposure and 1.0 x 10(-7) m, double exposure), when compared with u ... | 1987 | 3651887 |
diagnosis of bovine respiratory syncytial virus infections improved by virus detection in lung lavage samples. | the potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (brsv) was investigated. the technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. in 9 epizootics of respiratory tract disease, brsv infection was diagnosed in 1 or more calves. the brsv was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus ... | 1986 | 3511803 |
t lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection. | following a primary bovine herpesvirus-1 (bhv-1) infection the concanavalin a (con a) induced proliferative responses of peripheral blood t lymphocytes were suppressed. this suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. however, regression analysis demonstrated a significant correlation between the percentage of t lymphocytes present within the peripheral blood mononuclear cell (pbmc) population and the ... | 1987 | 3509949 |
bovine peripheral blood leukocyte subpopulation dynamics following a primary bovine herpesvirus-1 infection. | population dynamics of bovine peripheral blood leukocyte subpopulations were quantitated following a primary bovine herpesvirus-1 (bhv-1) infection. percoll isolated peripheral blood mononuclear cell (pbmc) subpopulations were analyzed using flow cytometry (fc) and cytochemical stains. between days two to eight post-infection (pi) there was a significant decrease in the percentage of t-cells and nont/nonb cells which was accompanied by an increased percentage of b-cells and monocytes. these perc ... | 1987 | 3509948 |
cytotoxicity of bovine lymphocytes after treatment with lymphokines. | cytotoxic lymphocytes were generated from bovine peripheral blood mononuclear leukocytes after in vitro stimulation with lymphokines that contained interleukin-2. lymphokine-stimulated cultures were cytotoxic to k562 cells (human natural killer [nk] targets) and yac-1 cells (mouse nk targets), but not to hsb-2 cells (human nk targets) in a 4-hour, 51cr-release assay. cells generated after lymphokine activation also mediated antibody-dependent cellular cytotoxicity to hsb-2 cells. appearance of e ... | 1986 | 3488701 |
early embryonic death in heifers after inoculation with bovine herpesvirus-1 and reactivation of latent virus in reproductive tissues. | thirteen crossbred heifers seronegative for bovine herpesvirus-1 (bhv-1) were bred naturally to a seronegative bull. eight heifers were inoculated with bhv-1, iv, on postbreeding day (pbd) 7 or 14. viremia was detected in heifers 1 through 7, and virus also was isolated from nasal and vaginal secretions of heifers 2, 3, 4, 6, and 7. the pregnancy status of all heifers was monitored from pbd 14 to pbd 35 by determining plasma progesterone concentrations at 1- to 3-day intervals. decreased progest ... | 1987 | 3434897 |
validation of disease diagnoses reported to the national animal health monitoring system from a large colorado beef feedlot. | clinical observation and collection of biological specimens from a large beef feedlot (approximately 30,0000 animals) were used to evaluate 6 approaches for validation of a disease reporting system. data collected during a 12-month period were used to evaluate each approach. a subsample of disease cases reported to the national animal health monitoring system (nahms) was compared with the clinical observations of the investigators. although the agreement between clinical diagnosis by the nahms v ... | 1988 | 3372333 |
vaccination studies against experimental bovine pasteurella pneumonia. | vaccination-challenge experiments were conducted in colostrum-deprived calves to evaluate the efficacy of pasteurella bacterins and vaccines against experimental pneumonic pasteurellosis. calves were vaccinated with formalin-killed bacterins and live vaccines, then challenge exposed intratracheally with p. haemolytica or p. multocida. infectious bovine rhinotracheitis virus was inoculated intranasally three to four days prior to p. haemolytica challenge-exposure. all calves were examined for mac ... | 1987 | 3300921 |
efficacy of a streptomycin-dependent, live pasteurella haemolytica vaccine against challenge exposure to pasteurella haemolytica in cattle. | a streptomycin-dependent, live pasteurella haemolytica vaccine was given in 1 or 2 doses to 2 groups of weaned calves; 2 other groups of calves were not vaccinated. all calves in the vaccinated groups and calves in 1 of the nonvaccinated groups were stressed by transport, intratracheally inoculated with bovine herpesvirus type-1 (cooper strain), and then intratracheally inoculated with p haemolytica type a1. the 4th group of calves (nonvaccinated controls) was not stressed and were not intratrac ... | 1987 | 3296883 |
activation of protein breakdown and prostaglandin e2 production in rat skeletal muscle in fever is signaled by a macrophage product distinct from interleukin 1 or other known monokines. | during sepsis or after injection of endotoxin into rats, there is a large increase in muscle protein breakdown and prostaglandin e2 (peg2) production. prior studies showed that partially purified interleukin 1 (il-1) from human monocytes can stimulate these processes when added to isolated rat muscles. the availability of pure recombinant il-1 and other monokines has allowed us to investigate the identity of the active agent in this process. incubation of muscles with recombinant human or murine ... | 1988 | 3284911 |
nitrogen kinetics of infectious bovine rhinotracheitis-stressed calves. | eight crossbred feeder steers were used in two consecutive n balance studies to investigate the effects of infectious bovine rhinotracheitis virus (ibrv) on n kinetics. balance study 1, which followed a 10-d acclimation phase, consisted of 7 d of sample collection referred to as the healthy phase (d -7 to d -1). study 2, the ibrv-infected phase, began 2 d after a nasal ibrv challenge and continued for 6 d (d 2 to d 8). a stable isotope, [15n]-glycine, was used to determine n kinetics in both stu ... | 1988 | 3209506 |
reproductive difficulties in cattle with antibody titers to haemophilus somnus. | a herd of holstein cows was examined because of suspected embryonic death. four cows had embryonic loss, and 2 cows had aborted. paired serum samples were tested for antibodies to infectious bovine rhinotracheitis virus, bovine viral diarrhea virus, and haemophilus somnus. of 16 cows, 8 had antibody titers to h somnus greater than 1:1,024, and 3 had greater than or equal to four-fold changes in antibody titers to h somnus. haemophilus somnus infection was active in this herd and may have been re ... | 1988 | 3192475 |
prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle. | the specificity of serum antibodies for the polypeptides of bovine respiratory syncytial virus (brsv) was examined, using sera obtained from feedlot and range cattle. test results in sera from feedlot cattle indicated a 60% rate of seroconversion and 95% seropositivity to brsv, associated with lack of clinical signs indicative of respiratory tract disease. exposure to other common respiratory tract viruses also was high (greater than or equal to 92% to bovine herpesvirus type 1, bovine viral dia ... | 1988 | 3178027 |
[evaluation of synthetic activity of lymphocyte nucleoli and phagocytic activity in peripheral blood leukocytes in heifers after infection with infectious bovine rhinotracheitis virus]. | the biosynthetic activity of the nucleoli of peripheral blood lymphocytes in calves, and the proportion of lymphocytes with the micronucleoli, with compact and ring-shaped nucleoli, were evaluated after experimental intranasal and intratracheal infections with the infectious bovine rhinotracheitis (ibr) virus. ibr virus belongs to the group of herpes viruses, the control of which is dominated by cellular immunity. the results are compared with the values for phagocytizing neutrophils, phagocytic ... | 1987 | 3122402 |
comparison of five cell lines for the propagation of bovine viral diarrhea and infectious bovine rhinotracheitis viruses. | a comparative study was carried out to determine the susceptibility of 5 different cell types to bovine viral diarrhea (bvd) and infectious bovine rhinotracheitis (ibr) viruses. the cell systems used were swine testicle (st), mink lung (ml), bovine turbinate (bt), porcine kidney (pk15) and equine dermal (ed) cells. for bvd virus, the titers obtained on day 8 post-infection were 10(1.13), 10(3.25), 10(4.13), 10(0.00) and 10(0.00), in st, ml, bt, pk15 and ed cells, respectively. for the ibr virus, ... | 1987 | 3041184 |
[bhv-1 infection of cattle: stabilization by active immunization of seropositive animals with inactivated vaccine--a field trial]. | 1987 | 3040371 | |
elisa for bovine herpesvirus 1 (bhv1) antibody with hirt supernatant. | detailed studies were made of the ability of hirt supernatant (hs) from bovine herpesvirus 1 (bhv1) infected cultures to sensitize plates for enzyme-linked immunosorbent assay (elisa). the ultracentrifuged pellet of hs had less sensitizing activity than the supernatant, but the antigen was removed completely by 0.22 micron filters and to some extent by 0.45 micron filters; it was minimally affected by sonication; it was destroyed by the action of pronase but not by dnaase i when a kallikrein ina ... | 1987 | 3038916 |
use of recombinant bovine alpha 1 interferon in reducing respiratory disease induced by bovine herpesvirus type 1. | intranasal or intramuscular treatment of calves with recombinant bovine alpha 1 interferon before challenge with bovine herpesvirus type 1 and pasteurella haemolytica reduced clinical signs, number of sick days, lung lesions, and weight loss. the effective dose was determined to be relatively broad within the range of 1 to 50 mg per animal. no adverse effects were observed even at high doses of interferon (50 mg per animal). administration before virus infection was more effective than administr ... | 1987 | 3038008 |
detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure. | a sensitive and specific procedure for the detection of argentine isolates of bovine herpesvirus-1 was developed. the procedure was based on a dot-blot, nucleic acid hybridization, using 32p, nick-translated, plasmidic probes. the probes contained cloned bam h1 restriction fragments in the left half of the viral genome. the detection limit of the procedure was 10 pg of viral dna. | 1987 | 3037953 |
protection of cattle from bovine herpesvirus type i (bhv-1) infection by immunization with individual viral glycoproteins. | the major glycoproteins gi, giii, and giv of bovine herpesvirus-1 (bhv-1) were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of bhv-1-infected cells. immunized animals were fully protected from disease, using a bhv-1/pasteurella haemolytica aerosol challenge model but not from infection with the virus. thus, virus could still replicate in the nasal passages of immunized animals, although to a lesser extent t ... | 1987 | 3037783 |
the genome of caprine herpesvirus 1: genome structure and relatedness to bovine herpesvirus 1. | caprine herpesvirus 1 (caphv-1) dna was examined by electron microscopy, restriction site mapping and homology studies with bovine herpesvirus 1 (bhv-1) dna. although the restriction site maps differed significantly, we showed that the genome structures of caphv-1 and bhv-1 were identical and that the dnas shared a high degree of base sequence homology. | 1987 | 3037021 |
viral-bacterial pneumonia in calves: effects on plasma eicosanoids and long chain fatty acids. | in the pathogenesis of bovine pneumonic pasteurellosis, immunodepression induced by stress or respiratory viral infection permits superinfection of the lungs with pasteurella hemolytica, which results in exudative fibrinous pneumonia. therefore, bovine pneumonic pasteurellosis was induced by sequential inoculations of calves with bovine herpes virus-1 (bhv-1, 3 x 10(7) tissue culture infectious dose 50 (tcid50)/nostril), followed 3 days later by challenge with p. hemolytica (15 x 10(9) colony-fo ... | 1987 | 3036736 |
comparison of stationary and roller cultures for the isolation of herpesviruses affecting livestock. | the effect of rotating cultures on the isolation and cultivation of pseudorabies, infectious bovine rhinotracheitis and equine rhinopneumonitis viruses was studied. serial ten-fold dilutions of laboratory strains of these viruses and field strains of pseudorabies virus were inoculated in two each of roller and stationary tubes of appropriate cell cultures. all tubes were examined daily for the appearance of cytopathic effects. roller cultures were found to slightly enhance the speed and extent o ... | 1987 | 3035348 |
reactivation of infectious bovine rhinotracheitis virus by transport. | transport was studied as a cause of reactivation of infectious bovine rhinotracheitis virus (bovine herpesvirus-1; bhv-1) in heifers vaccinated 2-6 months before transport, using a double dose of the thermosensitive (ts) vaccine strain (tracherine). eight out of 19 animals showed ts strain re-excretion over a period of 1-3 days, beginning, in 5 out of the 8 heifers, the day after transport. in 14 other heifers, only sera were examined by sero-neutralisation: only 1 out of these 14 animals showed ... | 1987 | 3034502 |
reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (bhv-1) strain and to proteins of representative bhv-1 strains. | 15 monoclonal antibodies (mcabs) were induced with the neuropathogenic strain n 569 of bovine herpesvirus 1 type 3 (bhv-1.3). nine of them could be shown by radioimmunoprecipitation assay to react with viral glycoproteins and two of these mcabs were able to neutralize strain n 569. the reactivity of these 15 monoclonals was compared with 11 monoclonal antibodies induced with a bhv-1.1 strain. the available monoclonal antibodies made it possible to characterize bhv-1.3 and to classify bhv-1 into ... | 1987 | 3034198 |
reactivation in calves of latent infection by bovid herpesvirus-4. | nine calves, six of which had been infected with strain 85/bh 16tv and three with strain 85/bh 232tn of bovid herpesvirus-4 (bhv-4), were treated with dexamethasone (dms) three months after infection. dms administration did not induce any clinical signs of disease, but bhv-4 was isolated from the nasal swabbings of all calves for a maximum of 8 days after the start of dms treatment. the virus was also isolated from the nerve tissues, nasal mucosa, lymph nodes, lung and spleen of 4 calves that we ... | 1987 | 3033450 |
highly sensitive antigen detection procedures for the diagnosis of infectious bovine rhinotracheitis: amplified elisa and reverse passive haemagglutination. | the sensitivity of an enzyme-linked immunosorbent assay (elisa) for the detection of bovid herpesvirus 1 antigen was increased by up to 50-fold using the biotin-avidin interaction to amplify the reaction, when compared with a simple sandwich elisa. an alternative immunoassay, reverse passive haemagglutination (rpha), had a similar sensitivity to the amplified elisa, and was technically simpler to perform. both the amplified elisa and the rpha could detect viral antigen in the nasal secretions of ... | 1987 | 3031873 |
hemagglutination inhibition and virus neutralizing response of rabbits inoculated with bovine herpesvirus 1 subunit vaccine. | the immunogenicity of bovine herpesvirus type 1 (bhv-1) hemagglutinin has been investigated. both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (hau) were injected subcutaneously into rabbits. both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either triton x-100- or octylglucoside-solubilized subunit vaccine. blotting analysis ... | 1987 | 3031871 |
inheritance and interaction of immune traits in beef calves. | three sets of blood samples were obtained from beef calves of two experimental populations and assayed for various immunological measurements. the first set of samples was taken between 24 and 48 h after birth and quantified for igg1 concentration. a second set was taken immediately prior to vaccination for infectious bovine rhinotracheitis virus (ibrv; at an average age of 164 d) and a third set taken 60 d post-vaccination. these later samples were quantified for antibodies specific to ibrv. le ... | 1987 | 3030992 |
natural cell-mediated cytotoxicity to cells infected with infectious bovine rhinotracheitis virus. | cell-mediated cytotoxicity against viral-infected cells was demonstrated in a 6-hr 51cr release assay. peripheral blood mononuclear leukocytes from both infectious bovine rhinotracheitis virus (ibrv)-infected and noninfected cattle exhibited preferential lysis against ibrv-infected primary bovine embryonic kidney (bek) cells compared to cells infected with pseudorabies virus and noninfected bek cells. addition of specific antibody to the assay did not enhance cytotoxicity. the effector cell was ... | 1987 | 3029944 |
patterns of bovine t cell-mediated immune responses to bovine herpesvirus 1. | lymphocyte proliferation was used to evaluate t cell-mediated immune responses to different isolates of bovine herpesvirus 1 (bhv-1). groups of high, moderate, and low responses were observed when lymphocytes from three breeds of dairy cattle were stimulated with each of the bhv-1 isolates. proliferation of cells in the low responding group could be augmented by exogenous il-2. the mechanism of unresponsiveness by cells from one individual whose response was not altered by il-2 supplementation w ... | 1986 | 3029942 |
[bvd (bovine virus diarrhea) and ibr (infectious bovine rhinotracheitis): serologic studies on 35 farms]. | 1099 female cattle from 35 farms in the provinces of north brabant, gelderland and overijssel were examined for the presence of antibodies against bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) by serum neutralisation tests. one of the farms examined was seronegative for bvd and 11 farms were seronegative for ibr. on the seropositive farms about 50% of cattle carrying their first calf had not experienced infection i.e. were seronegative (bvd 51.2%, ibr 48.3%). after cal ... | 1986 | 3027917 |
an evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle. | more than 300 bovine sera from a previously reported vaccination and challenge trial were tested for antibodies to bovine herpesvirus 1 (bhv1) by five serological assays: enzyme-linked immunosorbent assay (elisa) for igm and igg, passive haemagglutination (pha), and two methods of virus neutralisation (vn). in a statistical comparison of elisa (igg), pha and vn results, the assays showed highly significant correlations (p less than 0.01). the sensitivities of elisa and 24-hour neutralisation tes ... | 1986 | 3027800 |
intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. | monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (bhv-1) involved in virus neutralization were used in indirect immunofluorescence (iif) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells. antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (pi), followed by staining of the perinuclear region, presumably the golgi appara ... | 1987 | 3026288 |
comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals. | the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ... | 1987 | 3026244 |
restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands. | restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (bhv-1) isolate, originally obtained from bovine mammary gland lesions, with a standard bhv-1 strain, infectious bovine rhinotracheitis virus. although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by bam hi, eco ri, hind iii, and hpa i, revealed definite differences b ... | 1986 | 3026212 |
the genome of bovine herpesvirus 1 (bhv-1) strains exhibiting a neuropathogenic potential compared to known bhv-1 strains by restriction site mapping and cross-hybridization. | bovine herpesvirus 1 (bhv-1) strains can be differentiated by their dna and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. we classified the bhv-1 strains according to these data as bhv-1.1, bhv-1.2 (a/b) and bhv-1.3 (a/b). bhv-1.1 and bhv-1.2 correspond to the well known 'common' bhv-1 strains, whereas bhv-1.3 has only recently been recognized and exhibits a neuropathogenic potential. in the present paper we describe the structural genome characteris ... | 1986 | 3026111 |
cell-mediated cytotoxicity of peripheral blood mononuclear cells stimulated in vitro for infectious bovine rhinotracheitis virus-infected cells. | peripheral blood mononuclear cells (pbmc) from calves infected with and hyperimmunized to infectious bovine rhinotracheitis virus (ibrv) were stimulated in vitro with viral antigens to evaluate their cytotoxicity for a variety of cells. the 51-cr release assay was used to measure cytotoxicity. cytotoxicity was not present in fresh nonstimulated cells, but was detected in cultured, ibrv-stimulated cells at day 3, was maximal at day 7, and declined thereafter. pbmc stimulated in vitro with ibrv ex ... | 1986 | 3026081 |
reduction in in vivo testing at the national veterinary services laboratories of the united states department of agriculture. | efforts are being made at the national veterinary services laboratories to reduce in vivo testing of usda licensed veterinary vaccines. a hemagglutination test for determining potency of killed parvovirus vaccine is currently being used for canine and swine adjuvanted and nonadjuvanted products; a serum neutralization inhibition test (snit) is being developed for potency testing of killed adjuvanted infectious bovine rhinotracheitis (ibr), bovine virus diarrhea (bvd) and parainfluenza (pi3) vacc ... | 1986 | 3025035 |
in vitro induction of cytotoxic lymphocytes from infectious bovine rhinotracheitis virus hyperimmune cattle. | cytotoxic lymphocytes against infectious bovine rhinotracheitis virus (ibrv)-infected cells were induced by in vitro stimulation with ibrv antigen of peripheral blood leukocytes obtained from hyperimmune cattle. after one in vitro stimulation with ibrv, cytotoxicity was detected against ibrv-infected autologous target cells, but not against heterologous ibrv-infected or k562 target cells. after 4 in vitro ibrv stimulations at weekly intervals (in the presence of autologous feeder cells followed ... | 1986 | 3024535 |
detection of bovine herpesvirus type 1 rna in trigeminal ganglia of latently infected rabbits by in situ hybridization. | at times after conjunctival inoculation with bovine herpesvirus type 1 (bhv-1), representing the acute and latent phases of infection, rabbit trigeminal ganglia were examined for the presence of bhv-1 nucleic acids by in situ hybridization using a 3h-labelled bhv-1 dna probe. during the acute phase of virus infection, both bhv-1 dna and rna were detected in ganglionic neurons and occasionally in adjacent satellite cells. however, during the latent phase of infection only viral rna was detectable ... | 1986 | 3023537 |
identification of the thymidine kinase gene of infectious bovine rhinotracheitis virus and its function in escherichia coli hosts. | in an attempt to understand the gene expression of the infectious bovine rhinotracheitis virus (ibrv), the viral thymidine kinase gene (tk), a well regulated viral gene has been chosen for this study. a cosmid library of ibrv has been constructed in escherichia coli hb101 by cloning partially sau3a-digested dna fragments into a cosmid vector, pjb8. recombinant cosmids were further analyzed by restriction digestions and by southern blot hybridization. results showed that this cosmid library compr ... | 1986 | 3023190 |
[prevalence of bovine herpesvirus 4 in the swiss cattle population and possible serologic cross reaction with bovine herpesvirus 1 (ibr/ipv virus)]. | 1986 | 3022374 | |
map location of the gene for a 130,000-dalton glycoprotein of bovine herpesvirus 1. | a bovine herpesvirus 1 variant (mar6) containing a mutation in a viral glycoprotein with a molecular weight of 130,000 (g130) was isolated by selecting for resistance to a neutralizing monoclonal antibody (130-6) directed against g130. mar6 was completely resistant to neutralization by monoclonal antibody 130-6 in the presence and absence of complement, but was neutralized by polyvalent immune sera. the mar6 mutant synthesized and processed g130, but produced plaques which failed to react with m ... | 1986 | 3021976 |
detection of impaired t cell-mediated immune responses to herpesvirus (bhv-1) in cattle. | interleukin 2 (il-2) functions in the regulation of cell-mediated immune responses both in vivo and in vitro. therefore, il-2 production has been studied in patients with immunological disorders to determine the level of the immune defect. however, the cause(s) of low responses to selected antigens by cells from clinically normal individuals has not been examined. the ability of cells from clinically normal individual cattle to produce and respond to il-2 was investigated as a measure of specifi ... | 1986 | 3020771 |
[ibr/ipv serodiagnosis with elisa methods in blood, single milk and bulk milk samples; control measures for maintaining normal cattle herds; eradication measures with reference to vaccination]. | 1986 | 3019627 | |
investigation of possible vaccine-induced epizootics of infectious bovine rhinotracheitis, using restriction endonuclease analysis of viral dna. | viral dna was extracted from each of 14 modified-live (ml) bovine herpesvirus 1 vaccines, representing all of the ml infectious bovine rhinotracheitis virus (ibrv) vaccines licensed by the us department of agriculture for use in cattle. restriction endonucleases pst i and bgl ii were used to establish restriction enzyme patterns for the vaccinal viruses. viral dna from isolates obtained from 6 field samples of ibrv (1 from colorado, 1 from west virginia, 3 from wisconsin, 1 from south dakota) we ... | 1986 | 3019192 |
immunologic comparison of the proteins of pseudorabies (aujeszky's disease) virus and bovine herpesvirus-1. | the immunologic relationship between bovine herpesvirus-1 and pseudorabies virus was examined by 80% serum cross-neutralization test, enzyme-linked immunosorbent assays, and western immunoblotting procedures. immunogenic cross reactivity between the 2 viruses was observed with both the serum-neutralization test and the enzyme-linked immunosorbent assay. a probing of viral western immunoblots with rabbit hyperimmune antiserum showed that there were a number of viral-specific cross-reactive protei ... | 1986 | 3019188 |
the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus 1 vaccination. | five calves were given live intranasal vaccine against bovid herpesvirus 1 (bhv1) two days after intranasal inoculation of bovine pestivirus (bvdv). another 5 were vaccinated in the absence of bvdv. control unvaccinated groups were also maintained. all calves were challenged with virulent bhv1. the unvaccinated calves developed signs of infectious bovine rhinotracheitis (ibr) and both vaccinated groups showed a similar degree of clinical protection from ibr. those given bvdv before vaccination s ... | 1986 | 3016976 |
identification of bovine herpesvirus-1 polypeptides involved in serum neutralization. | bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with nonidet p-40. the crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page) and western ... | 1986 | 3016973 |
enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
influence of induced disease states on the disposition kinetics of imidocarb in goats. | the influence of fever, induced by different agents, on the disposition kinetics of imidocarb was determined in goats. escherichia coli endotoxin (0.2 microgram/kg), trypanosoma evansi (10(7) in 1 ml sterile glucose citrate), and infectious bovine rhinotracheitis virus (10(6.5)tcid50) were the agents administered to induce the febrile state. in control and febrile animals the two-compartment model was used to describe the disposition kinetics of the drug. fever caused significant changes to occu ... | 1986 | 3014166 |
nitrogen metabolism of calves inoculated with bovine adenovirus-3 or with infectious bovine rhinotracheitis virus. | beef calves were inoculated with bovine adenovirus-3 or infectious bovine rhinotracheitis virus. after inoculation, plasma fibrinogen increased, serum phosphorus decreased, and nitrogen and phosphorus digestibility decreased compared with preinoculation values. urinary n excretion increased when calves developed rectal temperatures greater than 39.7 c. results indicated that clinical infection of calves with infectious bovine rhinotracheitis virus increases urinary n excretion and reduces n and ... | 1986 | 3013053 |
neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus. | infectious bovine rhinotracheitis virus (ibrv) has been shown in this report to have thirty-three polypeptides. ten of the eleven polypeptides which can be labeled with (3h)-glucosamine are located on the surface of the virus since they can be surface labeled with sodium boro(3h)hydride. in order to define the immunologically important viral proteins, monoclonal antibodies were prepared against the virus and selected for their ability to neutralize infectivity. four such hybridoma lines were obt ... | 1986 | 3010905 |
development of specific antibodies mediating antibody-dependent cell-mediated cytotoxicity following experimental infection of cattle with bovine herpesvirus 1 and subsequent viral reactivation. | evolution of sensitizing antibodies involved in antibody-dependent cell-mediated cytotoxicity (adcc), antibody-complement cell lysis and complement-facilitated adcc was followed in bulls after primary infection by a wild strain of infectious bovine rhinotracheitis virus (bovine herpesvirus 1; bhv-1) and after experimental reactivation of the virus. these antibodies were detected between the 4th and the 7th day after primary infection, reached a maximum level after 2 weeks and rose slightly after ... | 1986 | 3010549 |
[comparison of the immunizing effectiveness of subunit vaccine and inactivated viral oil vaccine against infectious rhinotracheitis of cattle]. | trials were conducted on rabbits and cattle to compare the immunizing effectiveness of the subunit vaccine against infectious bovine rhinotracheitis (ibr), representing antigens separated by the solubilization of the ibr virus-infected cells by means of triton x-100 with oil adjuvant, with the inactivated oil ibr vaccine. the rabbits inoculated and re-vaccinated with both vaccines in an interval of three weeks produced neutralizing antibodies in medium titres, the values of these antibodies were ... | 1986 | 3010533 |
logical description of bovine herpesvirus type 1 latent infection. | description of the interactions between bovine herpesvirus type 1 (bhv-1) and cattle was performed by the method known as kinetic logic. this logical formalization uses variables with two possible values, 1 and 0, which tell whether an element is present or not at a significant level. to each variable is associated a function which tells if the element is being produced at a significant rate. the temporal relation between a variable and its associated function is given by specific time delays. t ... | 1986 | 3009689 |
polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1. | the polypeptide specificities and defense mechanisms of the humoral immune response to bovine herpesvirus 1 were analyzed. sequential serum samples taken from cows which were experimentally infected with bovine herpesvirus 1 were tested for their reactivity with individual bovine herpesvirus 1 polypeptides by immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay. all bovine immune sera reacted with each of the three major bovine herpesvirus 1 glycoproteins, gvp 6/11a/16, gvp ... | 1986 | 3009533 |
effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
detection of bovine herpesvirus-specific nucleic acids by in situ hybridization with biotinylated dna probes. | biotin-labeled dna probes for bovine herpesvirus type 1 (bhv-1) were used to detect viral nucleic acids in infected cell cultures and clinical specimens by in situ hybridization. hybridization signal was detected 2 hours after inoculation in the cytoplasm of infected cells, presumably representing input virus. hybridization was first detected in the nucleus at 4 hours after inoculation; by 10 hours after inoculation, hybridization signal was detected in both nucleus and cytoplasm of almost 50% o ... | 1986 | 3008605 |
intramuscular and intravaginal vaccination of pregnant cows with thymidine kinase-negative, temperature-resistant infectious bovine rhinotracheitis virus (bovine herpes virus 1). | to test the safety and efficacy of a thymidine kinase-negative (tk-), temperature-resistant (tr) mutant of bovine herpes virus-1 (bhv-1) in pregnant cows, seronegative cows, 2-5 months pregnant, were vaccinated intramuscularly (i.m.) or intravaginally (i.vag.) with this candidate vaccine virus. i.m. vaccinated cows did not shed virus i.vag. or intranasally (i.n.), but i.vag. vaccinated cows replicated virus i.vag. for 8-9 days postvaccination (p.v.) some of the cows were challenge exposed i.n. a ... | 1986 | 3008461 |
bovine herpesvirus-1 and pasteurella haemolytica aerobiology in experimentally infected calves. | eight calves (2 calves in each of 4 groups) were exposed to an aerosol of bovine herpesvirus-1 (bhv-1) and 4 days later to an aerosol of pasteurella haemolytica. samples of tracheal and exhaled air were taken simultaneously beginning 1 day before viral exposure and once a day up to 3 to 4 days after the bacterial exposure. samples were also taken during the period of aerosol exposure. only 0.04% to 0.42% of p haemolytica-carrying droplets of the bacterial aerosol passed beyond the cranial part o ... | 1986 | 3006556 |
study of immunity induced by the inactivated oil-adjuvant vaccine after natural infection with the virus of infectious bovine rhinotracheitis (ibr) in cattle. | 1985 | 3006406 | |
hemagglutinating activity associated with bovine herpesvirus type 1. | using c57bl/hpb mouse erythrocytes, hemagglutination has been observed with the los angeles and colorado-1 strains of bovine herpesvirus type 1 and with 12 other canadian field isolates as well. the specificity of the hemagglutination observed with the viral strains has been confirmed by a hemagglutination-inhibition assay. | 1985 | 3006322 |
occurrence of antibodies to the etiologic agents of infectious bovine rhinotracheitis, parainfluenza 3, leptospirosis, and brucellosis in white-tailed deer in minnesota. | a serologic survey was conducted on 628 white-tailed deer (odocoileus virginianus) in 1976 and 1979-1980. tests for antibodies to the etiologic agents of infectious bovine rhinotrancheitis (ibr), parainfluenza 3 (pi3), leptospirosis, and brucellosis produced positive results of 15%, 20%, 3% and 0%, respectively. adult deer had significantly higher prevalence of antibodies to ibr virus and pi3 virus than fawns. these data provide a basis for monitoring these disease agents in minnesota's white-ta ... | 1986 | 3005666 |
short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1. | analysis of the genomes of different bovine herpesvirus 1 strains revealed a ul terminal hindiii fragment differing in size (from 2.4 to 2.8 kilobases). this fragment polymorphism occurred in the dna of a wild-type isolate, in highly passaged, apathogenic tissue culture derivatives, and in plaque-purified substrains. this heterogeneity was due to variations in the copy number of a 14-base-pair tandem repeat comprising the base sequence 5'-gctcctcctccctc-3', which also exists, with some differenc ... | 1986 | 3005657 |
characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods. | ten glycoproteins of molecular weights of 180,000, 150,000, 130,000, 115,000, 97,000, 77,000, 74,000, 64,000, 55,000, and 45,000 (designated as 180k, 150k, etc.) and a single nonglycosylated 107,000-molecular-weight (107k) protein were quantitatively removed from purified bovine herpesvirus 1 (bhv-1) virions by detergent treatment. immunoprecipitations with monospecific and monoclonal antibodies showed that three sets of coprecipitating glycoproteins, 180k/97k, 150k/77k, and 130k/74k/55k, were t ... | 1986 | 3005625 |
bovine herpesvirus 1: molecular and antigenic characteristics of variant viruses isolated from calves with neurological disease. | this report presents data showing that several virus isolates recovered in argentina, mainly from calves with non-purulent meningo-encephalitis, represent a hitherto unrecognized antigenic variant of bhv-1. the following experimental approaches have been adopted to demonstrate both the unique features among and the relatedness with bhv-1 of these isolates: i) crossed serum neutralization test with rabbit immune sera, ii) analysis by sds-polyacrylamide gel electrophoresis of radio-labeled virus i ... | 1986 | 3004386 |
propagation of infectious bovine rhinotracheitis (bovine herpes-1) virus in murine primary cell cultures. | virus synthesis in balb/c mouse lung and kidney primary cultures infected with infectious bovine rhinotracheitis (ibr) virus started between 6 and 8 hours after virus inoculation and reached a maximum titer of 5.5 log10 plaque forming units (pfu) at 48 hours postinfection (pi). cytopathic effect (cpe) in cell cultures occurred at 8-10 hours and over 90% of the cells had cpe by 48 to 72 hours pi. the bulk of the newly replicated virus (60-80%) was cell-associated as determined by plaque assay of ... | 1986 | 3004011 |
[excretion of ibr virus, especially in milk, in experimentally infected cows]. | 1985 | 3003895 | |
studies on bovine herpesviruses. part 1. isolation and characterization of viruses isolated from the genital tract of cattle. | herpesviruses, previously isolated from cattle (theodoridis, 1978), were further studied and provisionally placed in the bovid herpesvirus 4 (bhv-4) group. major differences were found between ibr-ipv (bhv-1) and bhv-4 virus strains. in mdbk cells, all bhv-4 strains started growing at the edges of the culture, the process progressing slowly until destruction of the cells was complete by the 10th day. bhv-4 strains failed to induce neutralizing antibodies in cattle, goats and rabbits. only the ad ... | 1985 | 3003650 |
molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses l and bovine encephalitis herpesvirus. | restriction endonuclease dna fingerprints of herpesviruses isolated from 3 unrelated epidemics of bovine encephalitis are similar to each other and totally different from bovine herpesvirus 1 (bhv1). herpesviruses, antigenically related to bhv1, isolated from goats and buffalo have distinct dna fingerprints. we propose that bovine encephalitis herpesvirus is prototypic of a new bovine herpesvirus type and that alpha herpes viruses from individual ruminant species are species specific. | 1985 | 3002311 |
seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
experimentally induced pneumonic pasteurellosis: dose-response relationships and protection against natural reinfection in calves. | calves were inoculated intranasally with 2 x 10(6.2) tissue culture infective doses of infectious bovine rhinotracheitis virus, followed in 7 days by intratracheal inoculations with 1 of 4 challenge doses of pathogenic pasteurella haemolytica. severity and duration of the ensuing clinical signs of respiratory tract disease were correlated with the challenge dose of bacteria. calves given 1 x 10(6) colony-forming units (cfu) of bacteria did not develop reliable clinical evidence of disease, where ... | 1985 | 3002216 |