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[dipetalonema dessetae in proechimys oris. ii. evaluation of the model for pharmacologic investigations of antifilarial chemotherapy (author's transl)].dipetalonema dessetae in proechimys oris is a new model of rodent feafilariasis with several interesting features with respect to its utilisation in antifilarial pharmacology. 1. in the first part of these studies, it has been shown that the final host of the filaria, a rodent, was easy to breed. a prolific mosquito aedes aegypti was an effective vector, and with the selected mode of infection i.e. 200 infesting larvae subcutaneously injected, 80% of the rodents were infected. 2. from 90 to 150 ...19827200173
evaluation of n-toluyl piperidine as an effective repellent against aedes aegypti (l). 19807191409
[distribution of aedes aegypti in china and its comprehensive control]. 19827185486
melanization and encapsulation in aedes aegypti and aedes togoi in response to parasitization by a filarioid nematode (breinlia booliati).in aedes aegypti (singapore strain), s refractory host, there was a marked progressive decline of developing larvae of breinlia booliati during the incubation period. however, in aedes togoi, a susceptible host, the filarial larvae developed normally and the number of larvae remained constant throughout the incubation period. encapsulation and melanization of b. booliati larvae in ae. aegypti and ae. togoi were studied. ae. togoi occasionally mounted a defence reaction to the infection. in ae. a ...19827177704
distribution and density of aedes aegypti (l) and aedes albopictus (skuse) in sarawak. 19827176997
effects of benzylphenol and benzyl-1,3-benzodioxole derivatives on fertility and longevity of the yellow fever mosquito (diptera:culicidae). 19827175006
multiplication of coxiella burnetii in aedes aegypti. 19827174009
the ultrastructure of the larval malpighian tubules of a saline-water mosquito.the larval malpighian tubules of the saline-water mosquito aedes taeniorhynchus were examined using light and electron microscopy. the tubules contain two cell types; primary cells and stellate cells. primary cells are characterized by their size (70 microns x 70 microns x 10 microns) and an abundance of intracellular membrane-bound crystals. two types of microvilli are found on the luminal surface of the primary cells: (1) small microvilli containing core microfilaments and extensions of endopl ...19827170712
field trials on the effectiveness of bioresmethrin reslin 10/10 (r) on aedes aegypti. 19827167094
well breeding behaviour of aedes aegypti. 19827166362
a kinetic and ultrastructural comparison of alphavirus infection of cultured mosquito and vertebrate cells.vero cells and aedes pseudoscutellaris cells showed rapid production of semliki forest virus (sfv) whereas in aedes aegypti and anopheles stephensi cells no rapid production of sfv was observed. ultrastructurally the only virally induced cell inclusion in early infection was the cytopathic vacuole type 1. later in infection, in mosquito cells, electron-dense bodies appear and budding of new virions appears to be very efficient. in vero cells large accumulations of envelope proteins and nucleocap ...19827154145
an efficient floating larval trap for sampling aedes aegypti populations (diptera: culicidae). 19827154026
transmission of venezuelan equine encephalomyelitis virus by aedes aegypti (diptera: culicidae) to mice previously exposed to vector antigens. 19827154017
laboratory evaluation of bacillus thuringiensis var. israelensis serotype h-14 against mosquito larvae with particular reference to aedes aegypti. 19827153478
electrophoretic variability in the phosphatase system of the yellow-fever mosquito, aedes aegypti.alkaline and acid phosphatases in five inbred strains of the yellow-fever mosquito, aedes aegypti, were compared by vertical polyacrylamide gel electrophoresis. 1. polymorphic and monomorphic zones of enzyme activity were observed among both alkaline and acid phosphatases. 2. in the alkaline phosphatase system, seven electrophoretic zones of enzyme activity were detected; all but two of the seven zones showed electrophoretic variability. 3. in the acid phosphatase system, four electrophoretic zo ...19827151398
dengue-2 vaccine: oral infection, transmission, and lack of evidence for reversion in the mosquito, aedes aegypti.the dengue-2 vaccine virus (s-1), and its parent virus (pr-159), were compared for their ability to infect orally, to replicate in, and subsequently to be transmitted by aedes aegypti mosquitoes. the vaccine virus was markedly less efficient in its ability to infect mosquitoes orally. after ingesting infectious bloodmeals containing 3, 7 to 8.2 log10mid50/ml of the respective viruses, 56% (220/396) of the mosquitoes became orally infected with the parent virus contrasted with 16% (66/397) for th ...19827149108
dengue-2 vaccine: infection of aedes aegypti mosquitoes by feeding on viremic recipients.colonized aedes aegypti mosquitoes were fed on voluntary recipients of an experimental, live, attenuated, dengue type 2 (pr 159/s-1) vaccine to estimate the frequency of vector infection and the stability of the virus in mosquitoes. two volunteers were viremic at the time of mosquito feeding, but only two of 114 mosquitoes that took a viremic blood meal became infected with the vaccine virus. strains of virus recovered from the bodies of the mosquitoes and the volunteer's blood retained the temp ...19827149107
field experiments on the control of aedes aegypti and culex quinquefasciatus by toxorhynchites rutilus rutilus (diptera: culicidae). 19827120310
estimation of the amount of venezuelan equine encephalomyelitis virus transmitted by a single infected aedes aegypti (diptera: culicidae). 19827120306
infection of aedes aegypti with zygotes of plasmodium gallinaceum fertilized in vitro.female gametes of plasmodium gallinaceum fertilized in vitro, cleaned of all other blood constituents, resuspended in blood, and fed to aedes aegypti through a membrane were infective. at the lowest zygote concentration, 10(4)/ml, nearly every ingested parasite produced an oocyst. as the concentration ingested increased, efficiency to infect diminished, until above 10(7) zygotes/ml the number of oocysts produced became constant. this method should be valuable for determining the nutrient require ...19827119992
linkage of an idh locus with sex and two lethals in aedes aegypti.a previously unmapped isocitrate dehydrogenase (dh) locus has been mapped on chromosome 1 in aedes aedes aegypti. no recombination between this and sex was observed in any of the several hundred instances where crossing over could have been detected. within an isofemale line from daker, two ldh-1 alleles are in linkage disequilibrium with two lethal loci. the two lethals complement one another, and show no recombination with sex or ldh-1. recombination between bz and ldh-1 was demonstrated, plac ...20107108187
susceptibility of culex quinquefasciatus, aedes aegypti, anopheles culicifacies and anopheles stephensi against insecticides. 19827106890
juvenile hormone and the development of ovarian responsiveness to a brain hormone in the mosquito, aedes aegypti. 19827106542
[on an outbreak caused by dengue type 4 virus, in thio, new caledonia. epidemiological and clinical aspects (author's transl)].after imported cases, a dengue outbreak, caused by type 4 virus, affected 11 per cent of the habitants of thio, new caledonia. distribution of cases by sex, by ethnic and age group, by locality and by week is established on cases confirmed by serology and/or by virus isolation. clinical aspect was classical but liver and digestive tract of patients were frequently affected. five haemorrhagic forms were observed. aedes aegypti was found in each visited house; pooled specimens were found infected ...19827105285
geographic and temporal patterns of genetic variation of aedes aegypti in new orleans.populations of aedes aegypti were collected in 1976 and 1979 from a number of different areas of new orleans and analyzed for genetic variation of 12 isozyme loci. although six loci were polymorphic, the majority of loci showed no significant genetic differentiation over time or between regions. the greatest amount of genetic differentiation was found between populations bordering the mississippi river and the two inland areas, mid-city and mount olivet. the low amount of genetic heterogeneity a ...19827102920
the infectivity of microfilariae of brugia pahangi of different ages to aedes aegypti.by transferring microfilariae of brugia pahangi which had been born over a 24-hour period in the peritoneal cavities of jirds (meriones unguiculatus) to the blood circulation of other jirds, infections of known age were tested for their ability to develop into third-stage larvae (l3) in mosquitoes aedes aegypti. microfilariae less than three days old were not able to develop to l3. microfilariae which had been in circulation for three days to six months were capable of developing if ingested.19827096971
utilization and metabolism of dietary sterols in the honey bee and the yellow fever mosquito.the honey bee, apis mellifera, does not convert c28 and c29 phytosterols to cholesterol as found in most previous studies of phytophagous or omnivorous insects, but instead the workers and queens selectively transfer 24-methylenecholesterol, sitosterol and isofucosterol from their endogenous sterol pools to the brood larvae regardless of the sterol in the worker diet. administering radiolabeled sterols by feeding and injection has made it possible to trace this selective transfer through a secon ...19827087696
host behaviour and mosquito feeding success: an experimental study.the susceptibility of laboratory rabbits to attack by mosquitoes (aedes aegypti) is found to be closely linked to their grooming activity. prevention of grooming significantly increases both the percentage of mosquitoes feeding and the size of blood meals. grooming responses intensify with parasite density, and lead to a slight decline in parasite feeding success and blood meal size. grooming efficiency varied between individual rabbits and also a consequence of experience, with rabbits improvin ...19827080144
genetic analysis of abnormal male sexual development in aedes aegypti and ae. mascarensis backcross progeny.when male hybrids of aedes aegypti females and a. mascarensis males were backcrossed to a. aegypti females, 32.8 percent of the male progeny exhibited abnormal sexual development, including failure of the terminalia to rotate, a split sternite of the eighth abdominal segment with partially duplicated telomeres, or feminization that gives rise to sterile intersexes. observations made on three morphological marker loci and five isozyme loci with characteristic electromorphs in the two parental spe ...20107069190
ultrastructural damage of in vitro cultured ookinetes of plasmodium gallinaceum (brumpt) by purified proteinases of susceptible aedes aegypti (l.).previous in vivo studies have implicated trypsin-like proteinases in the destruction of ookinetes of plasmodium gallinaceum in the gut of the susceptible mosquito aedes aegypti (gass 1977). an in vitro study has shown that the ookinetes are destroyed by crude extracts of blood-fed a. aegypti and that this destruction is largely inhibited by an inhibitor of mosquito proteinases, lima bean trypsin inhibitor (gass and yeates 1979). in the present study ookinetes of p. gallinaceum have been incubate ...19817034390
morphogenesis of yellow fever virus in aedes aegypti cultured cells. i. isolation of different cellular clones and the study of their susceptibility to infection with the virus.we report the isolation of 19 cellular clones of aedes aegypti and analyze their susceptibility to infection with yellow fever virus, in comparison with the uncloned cells. four types of clones were found, different in their morphology, their metabolism and their virus production. the c 17 clone produces the most virus, not only with respect to the other aedes aegypti clones, but also to other uncloned arthropod cells published in the literature. a cytopathic effect exists in the virus infected ...19817025663
[action of development inhibitors on aedes aegypti l. mosquitoes and rhodnius prolixus stal. bugs. iv. the activity of precocenes]. 20157024772
morphometric parameters of the midgut cells of aedes aegypti l. (insecta, diptera) under various conditions.previous morphometric or biochemical investigations have yielded different data on the distribution of free and membrane-bound ribosomes in midgut cells of aedes aegypti. in the present paper ribosomal distribution has been morphometrically analysed to determine whether different mosquito strains, different food and different narcosis used in these previous studies, and/or methodological errors, could account for the different results. most of the cellular parameters in the stomach epithelium of ...19817023689
scanning electron microscope study on larvae of wuchereria bancrofti from the vector and from experimental rodent hosts.the surface structures of larvae of wuchereria bancrofti from liberia were examined by scanning electron microscopy. for this study microfilariae from man, first-stage, second-stage, and third-stage larvae from the vector aedes aegypti, and fourth-stage larvae from the experimental rodent hosts mastomys natalensis and meriones unguiculatus were available. from these larvae the structure of the anterior ends, the cuticle in the midbody regions, and the posterior ends are presented. as an obvious ...19807003854
role of a modified ovitrap in the control of aedes aegypti in houston, texas, usa.a modified ovitrap from which larvae could not escape was employed for one year for the control of aedes aegypti at blossom heights, houston, texas. at the end of this period, the breteau index in the ovitrap area had declined by 36%, in contrast to a sharp increase of nearly 500% in the non-ovitrap area. the premises index in the ovitrap area remained unchanged, while the index in the non-ovitrap area increased by 440%. the problems associated with this control campaign and the use of these "au ...06980740
dengue haemorrhagic fever--a public health problem and a field for research.dengue haemorrhagic fever/dengue shock syndrome (dhf/dss) is an enigmatic and growing public health problem which is confined at present to countries of south-east asia. since 1956, over 350 000 patients have been hospitalized and nearly 12 000 deaths have been reported. dengue viruses, a group of four flaviviruses, are transmitted to man by aedes aegypti. currently, dengue viruses are actively transmitted in 61 countries which circle the globe in the tropical zone and have a combined population ...19806966540
linkage relationships between an esterase locus and group ii markers in the yellow fever mosquito, aedes aegypti (diptera: culicidae). 19816945437
anti-gamete monoclonal antibodies synergistically block transmission of malaria by preventing fertilization in the mosquito.experiments from our laboratory previously demonstrated that infected chickens immunized with gametes of the avian malaria parasite plasmodium gallinaceum were no longer infectious to the mosquito vector aedes aegypti and that this transmission-blocking immunity was mediated by antibody. to identify those antigens that are the targets of transmission-blocking immunity, hybrid mouse cell lines secreting monospecific antibodies to surface antigens on male and female gametes of p. gallinaceum have ...19806935685
brugia pahangi: growth improvement with lecithin in the diet of axenically reared hosts, aedes aegypti. 19816894428
macrogeographic genetic variation in a human commensal: aedes aegypti, the yellow fever mosquito. 19836884770
production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products.five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ...19836883226
inheritance of brown-eye and colourless-eye in the mosquito aedes aegypti.an autosomal recessive gene bw (probably closely linked to y on chromosome 2) gives a brown eye colour. when this gene and the sex-linked gene re (red-eye) are both homozygous, a colourless-eye results. brown-eye shows good viability and penetrance, and it has been maintained as a pure stock. colourless-eye shows 87% penetrance in both sexes, and the females are low in viability and fertility. a colourless-eye stock has been maintained by crossing colourless-eyed males to their heterozygous red- ...19836882068
the regulation of preimaginal populations of aedes aegypti l. (diptera: culicidae) on the kenya coast. i. preimaginal population dynamics and the role of human behaviour.on the kenya coast aedes aegypti breeds in pots maintained indoors for water storage. pupal numbers vary greatly from one pot to another. the rhythm of water replenishment and the accidental introduction of food into the pots regulate pupal numbers. when water replenishment occurs more often than once per week, pupal numbers are small. when replenishment occurs less often, both low and high pupal numbers may be observed. the presence or absence of larval food distinguishes pots of high versus lo ...19836882066
effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites.an investigation was carried out to examine the effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites. the sporozoites were obtained from freshly dissected salivary glands of aedes aegypti: their infectivity was assayed by their ability to produce a patent parasitaemia following their inoculation into day-old chicks. infectivity was abolished by treatment of the sporozoites with trypsin at a concentration of 100 micrograms ml-1 for 30 minutes at 30 degrees ...19836882061
an osmoregulatory syncytium and associated cells in a freshwater mosquito.in embryological terms the anal papillae are the product of eversion of the hindgut tissues. the rectum and the anal papillae have the same origin and have a marked structural similarity. the insect hindgut is very labile being able to produce salt transporting or 'chloride cells' from any of the tissues of which it is composed. the hindgut consists of four distinct regions: the ileum and part of the anal canal have a mechanical function, the rectum and the posterior anal canal contain transport ...19836879594
toxicity of bacillus thuringiensis var. israelensis crystals to aedes aegypti larvae: carbonate reversal.the toxicity of purified bacillus thuringiensis var. israelensis crystals to larvae of aedes aegypti could be reversed 100-fold by levels of k(2)co(3) as low as 0.15%.19836870244
[action of crystals of bacillus thuringiensis var. israelensis on the midgut of aedes aegypti l. larvae, studied by electron microscopy].ingestion of bacillus thuringiensis var. israelensis crystals by aedes aegypti larvae is followed by midgut epithelium disruption. earliest ultrastructural changes consist of an enlargement of intra- and intercellular spaces in the basal region of the cell. endoplasmic reticula disintegrate by forming spherical structures which increase in size during intoxication. mitochondria are transformed at first into a condensed form, then become swollen with the disappearance of internal cristae. in the ...19836870088
glutathione biosynthesis in the aging adult yellow-fever mosquito [aedes aegypti (louisville)].our previous findings [hazelton & lang (1978) fed. proc. fed. am. soc. exp. biol. 37(6), 2378 (abstr.)] demonstrated a senescence-specific decrease in glutathione (gsh) concentration in the yellow-fever mosquito aedes aegypti (louisville)]. as a possible mechanism for this change, gsh biosynthesis was investigated in adult mosquitoes of different ages through the life-span. biosynthesis was measured as the incorporation rate of [14c]glycine or [14c]cystine into glutathione. essential information ...06860301
glutathione s-transferase activities in the yellow-fever mosquito [aedes aegypti (louisville)] during growth and aging.our previous findings [hazelton & lang (1978) fed. proc. fed. am. soc. exp. biol. 37(6), 2378 (abstr.)] demonstrated aging-specific changes in glutathione concentrations in the yellow-fever mosquito [aedes aegypti (louisville)]. a possible mechanism could be increased utilization via glutathione s-transferase. thus glutathione s-transferase activities were measured in mosquito samples from the entire life span, including growth, maturity and senescence. methods were validated for the quantitativ ...06860300
transovarial transmission of dengue 2 virus by aedes aegypti in nature.dengue 2 virus was recovered from three of 123 pools of naturally infected aedes aegypti larvae (6,200 insects) collected from water containers in rangoon. the virus was also isolated from two of 76 pools (7,730 mosquitoes) of male ae. aegypti, collected as larvae and reared in the laboratory to adults. minimum field infection rates among these two groups of mosquitoes were 1:2,067 and 1:3,865, respectively. insect pools were inoculated into toxorhynchites splendens mosquitoes and dengue viral a ...19836859404
[effect of the larval density of aedes aegypti mosquitoes on their mortality as affected by bacillus thuringiensis berl. preparations]. 19836843501
[repellent properties of mannich bases and cresol and phenol derivatives for aedes aegypti l. mosquitoes and the flea xenopsylla cheopis roths]. 19836843496
larvicidal activity of bacillus thuringiensis subsp. israelensis, serovar h14 in aedes aegypti: histopathological studies. 19836841993
plasmodium gallinaceum: avian screen for drugs with radical curative properties.existing primary screens for radical curative antimalarial drugs fail to adequately detect many compounds which affect the latent, exoerythrocytic hypnozoite, the stage of the parasite responsible for relapse. at the same time, these screens falsely identify a wide range of compounds with no radical curative activity. the avian malaria, plasmodium gallinaceum, and aedes aegypti mosquitos were used in a screen which measures the effects of candidate compounds on gametocytes and their development ...19836832279
epidemiologic investigations of dengue infection in mexico, 1980.a binational investigation was conducted in two mexican cities in 1980 to study epidemiologic characteristics of dengue. two study areas were selected in each of the cities (merida and tampico); in each area, in february and in september, sanitarians recorded information concerning abundance of aedes aegypti, and public health nurses obtained blood specimens and clinical information from residents. ninety-nine individuals (24% of the study population) showed serologic evidence of recent dengue 1 ...19836829561
cryopreservation of the microfilariae of brugia malayi, dirofilaria corynodes, and wuchereria bancrofti.methods are presented for the cryopreservation of a sheathed microfilaria, brugia malayi, and an unsheathed species, dirofilaria corynodes. the former survived best when frozen at the rate of -0.8 degree or -0.5 degree c per minute using 9% dimethyl sulfoxide (dmso) as the cryopreservative. approximately 52-79% of the thawed microfilariae developed to the third stage in aedes aegypti mosquitoes versus 79% of the unfrozen specimens. for d. corynodes the optimum freezing rate was -2 degrees or -5 ...19836824119
comparative susceptibility of sterilized and genetically-defined strains of aedes aegypti to dirofilaria repens. 19816798102
dengue-2 vaccine: viremia and immune responses in rhesus monkeys.studies were undertaken in indian rhesus monkeys (macaca mulatta) to determine the safety, potency, immunogenicity, and mosquito infectivity of a small-plaque, temperature-sensitive variant of dengue type 2 (den-2) virus, a vaccine candidate. fifteen monkeys were inoculated subcutaneously with the vaccine virus, ten receiving 10(3.1) plaque-forming units (pfu) and five receiving 10(4.5) pfu. after primary immunization, viremia was detected in only one monkey, a recipient of the higher dose of va ...19806766903
transovarial transmission of yellow fever virus in stegomyia mosquitoes.transovarial transmission of two strains of yellow fever virus was demonstrated in three colonized geographical strains of aedes aegypti following infection by intrathoracic inoculation. infected progeny were detected in f1 offspring from only the first three ovarian cycles (ovc). the overall minimum filial infection rate for the first three ovc was 1:596; rates were loser in the second and third ovc. virus survived in eggs for longer than 4 months and was recovered from progeny of three immersi ...19806766282
comparative study of hemocytes and associated cells of some medically important dipterans.the aim of this work is to study, characterize, and compare different morphological types of hemocytes of glossina austeni, g. morsitans, calliphora erythrocephala, stomoxys calcitrans, lucilia sericata, aedes aegypti, and culex quinquefasciatus. this information is intended to provide a basis for future studies of the cellular defense mechanisms of these dipterans. seven morphological types of hemocytes were identified by phase-contrast optics: prohemocytes, plasmatocytes, thrombocytoids, granu ...19826764649
nondevelopment of wuchereria bancrofti in nude (congenitally athymic) mice.infective larvae of wuchereria bancrofti (nematoda: filarioidea) were harvested from aedes aegypti mosquitoes fed in vitro on microfilaremic blood of haitian volunteers. larvae were inoculated either into the subcutaneous tissue (sc) or peritoneal cavity (ip) of congenitally athymic (nude) mice, c3h/hen (nu/nu). initially 15 male and two female mice received larval doses from 38-180, and necropsy was done between 11 and 75 days post-inoculation. no worms were found at necropsy. four additional m ...19826751107
blood feeding affects age-related changes in the host-seeking behavior of aedes aegypti (diptera: culicidae) during oocyte maturation. 19846748001
experimental aerial spraying with ultra-low-volume (ulv) malathion to control aedes aegypti in buga, colombia. 19846733345
genetic heterogeneity among caribbean populations of aedes aegypti.using starch gel electrophoresis, we have analyzed genetic variation at 11 loci in 18 caribbean collections of aedes aegypti. our results show that, while there is some relationship between geographic proximity and genetic distance, the overall picture among islands is one of gene frequency patchiness , with some collections clearly not conforming to any geographic pattern. we attribute this to the combined effects of high rates of gene flow among islands and with the mainland american continent ...19846731681
[ecology of dengue and aedes aegypti. preliminary research. 2]. 19846729600
plasmodium gallinaceum: density dependent limits on infectivity to aedes aegypti.in acute, blood-induced infections of chickens, the malarial parasite plasmodium gallinaceum is most infective to the mosquito aedes aegypti 1 day before gametocyte numbers peak. in an effort to account for this disynchrony , daily changes in parasite infectivity, parasitemia, hematocrit, and hemoglobin were measured during the course of infections. three events were correlated with the loss of infectivity: (1) in the 24 hr between park infectivity and peak gametocytemia , schizont-induced hemol ...19846723894
a mosquito-virulent bacillus sphaericus in adult simulium damnosum from northern nigeria.from newly emerged adult simulium damnosum from kaduna river, northern nigeria, a mosquito-virulent strain of bacillus sphaericus was isolated. in another four cases non-virulent b. sphaericus were present. the isolate exerted a lc50 of 50 spores/ml for culex pipiens l4-larvae and was less virulent for anopheles stephensi (400 sp/ml) and aedes aegypti (800 sp/ml).19846720123
the use of mosquitoes (aedes aegypti) to detect ciguatoxin in surgeon fishes (ctenochaetus striatus).a new animal assay to detect ciguatoxin in fishes was developed. mosquitoes were subjected to intrathoracic injection of serial dilutions of ciguatoxin crude extracts. toxicity of the fishes was expressed as the mosquito ld50 (g of flesh per mosquito). a significant correlation between the mosquito bioassay and the mouse bioassay performed on the same extracts was shown (r = 0.72, n = 80). we were able to detect 96% of the toxic fishes, while 91% of the fishes non-toxic in mice were also non-tox ...19846719474
[effect of the mosquito cage temperature and the blood of the host food source on the formation and breakdown of the peritrophic membrane in female aedes aegypti l. (diptera, culicidae)]. 20096717378
[comparison of the flight capability of plasmodia-infected and plasmodia-free aedes aegypti]. 19846717377
plasmodium gallinaceum: erythrocyte factor essential for zygote infection of aedes aegypti.zygotes of plasmodium gallinaceum, fertilized in vitro and fed to aedes aegypti mosquitoes through a membrane, formed oocysts only when a substance in the cytoplasm of uninfected erythrocytes was present. the relation between erythrocyte volume and infectivity was linear (1:1.2) up to a 50% hematocrit. the intraerythrocytic substance was both nondialyzable and poorly soluble in plasma. by carboxymethyl cellulose chromatography, cytoplasmic constituents eluted at ph 8.6 supported the same infecti ...19846714357
the effect of humidity on the transmission of brugia pahangi infective larvae to mammalian hosts by aedes aegypti.the transmission of brugia pahangi by aedes aegypti to the mammalian host was compared at low and high humidity. there was no statistical difference between the number of infected mosquitoes feeding or the egress of infective larvae from these mosquitoes at high or low humidity. the penetration of the host by the infective larvae was however significantly greater (p less than 0.05) at high than at a low humidity.19846710572
a large transmissible plasmid is required for crystal toxin production in bacillus thuringiensis variety israelensis.bacillus thuringiensis var. israelensis (bti), serotype 14, which produces parasporal crystals toxic to certain dipteran larvae, was analyzed by agarose gel electrophoresis and found to contain a complex plasmid array. eight plasmids were detected, with approximate sizes of 3.3, 4.2, 4.9, 10.6, 68, 75, 105, and 135 mda, as well as a plasmidlike linear dna element of approximately 10 mda. partially cured mutants of bti implicated the 75-mda plasmid in crystal production. fifteen independently iso ...19846709732
bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads.solubilized crystals of bacillus thuringiensis var. israelensis were 7000 times less toxic to aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. a procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. the latex bead assay should make it possible to analyze the structure and mode of action of the mosquito t ...19846701520
dengue fever. reappearance in northern queensland after 26 years.during march, 1981, a number of cases of dengue fever occurred in cairns and townsville, northern queensland. from october, 1981, an outbreak of the infection was recognized on thursday island and, by may 1982, an estimated 38% of residents had been infected. isolated cases were reported from other towns in northern queensland and from other islands in the torres strait. clinical presentation varied from that of severe incapacitating illness lasting up to seven days to infections which were conf ...19846700459
increased intradermal probing time in sporozoite-infected mosquitoes.because malaria sporozoites destroy segments of the salivary glands of vector mosquitoes, we determined whether salivary function is impaired. such pathology would result in a prolonged intradermal probing phase of feeding behavior, because the role of saliva is to help locate blood vessels. indeed, non-infected aedes aegypti mosquitoes probed for a shorter period than did either sporozoite-infected or saliva-deprived mosquitoes. salivary apyrase activity is reduced to a third following maturati ...19846696175
characterization of antigens on mosquito midgut stages of plasmodium gallinaceum. i. zygote surface antigens.we have defined the surface protein antigens on plasmodium gallinaceum zygotes using radioiodination methods and rabbit anti-zygote serum which blocks transmission of the parasite to aedes aegypti mosquitoes. fifteen protein bands (1-15) in the molecular weight range of 40 000-240 000 and one band at the bromophenol blue dye marker were labelled by the lactoperoxidase and iodogen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril) methods. the localization of these radioiodinated components ...19836683783
insecticidal effect of aliphatic alcohols against aquatic stages of aedes mosquitoes.effectiveness of certain long carbon chain aliphatic alcohols as ovicides and larvicides was investigated under laboratory conditions against eggs and larvae of aedes aegypti and aedes scutellaris. out of the eight alcohols tested, three (1-decanol, 1-undecanol and 1-dodecanol) proved to be very effective. the hatching of mosquito eggs was inhibited at a dosage of 5 to 7 litres/hectare. first, third and fourth instar larvae were killed at a dosage of 3 to 7 litres/hectare. first instar larvae we ...19836683011
susceptibility of aedes aegypti to infections with dirofilaria immitis and dirofilaria repens.eight stocks of aedes aegypti-4 geographic stocks and 4 selected stocks-were tested for their susceptibility to infection with dirofilaria immitis and d. repens. four of the stocks were found to be susceptible and the other 4 were refractory of infection with d. immitis. all the stocks of ae. aegypti that were susceptible to d. immitis were also susceptible to d. repens but the stocks that were refractory to d. immitis were all partially susceptible to d. repens.19836673132
linkage relationships of eleven enzyme loci in the aedes scutellaris group.linkage relationships of 11 enzyme loci were determined in backcrosses between aedes polynesiensis and aedes kesseli. three linkage groups established were aat2-lap2-me-sex, cat-ao-pgm-idh2-est6, and gpi-odh-pgd. lap2 and cat have not been previously mapped in aedes. locus order and linkage groups were the same as those observed for seven loci mapped in aedes aegypti. the significance of the observed similarities in chromosome organization and differences in crossover values among closely relate ...19836670992
a case study of aedes aegypti prevalence by settlement types in dehu town group of maharashtra state. 19836668009
transmission of dengue virus by orally infected aedes triseriatus.transmission of dengue type 1 was demonstrated for 3 strains of aedes triseriatus mosquitoes after oral infection. rates of infection were similar to those observed in a control strain of aedes aegypti. three additional species belonging to the subgenus protomacleaya (aedes brelandi, aedes hendersoni, and aedes zoosophus) were also susceptible to oral infection with dengue type 1 virus but transmission could not be demonstrated although virus was detected in the salivary glands of infected mosqu ...19836650743
vertebrate host specificity and experimental vectors of plasmodium (novyella) kempi sp. n. from the eastern wild turkey in iowa.vertebrate host specificity, experimental laboratory vectors, and a description of plasmodium (novyella) kempi sp. n. from eastern wild turkeys (meleagris gallopavo silvestris vieillot) in iowa are presented. plasmodium kempi is infective for domestic turkeys, bobwhites (colinus virginianus), chukars (alectoris graeca), guinea fowl (numida meleagris), peacocks (pavo cristatus), and canaries (serinus canaria), produces a transient infection in mallards (anas platyrhynchos) and domestic geese (ans ...19836644918
[action of the delta-endotoxin of bacillus thuringiensis var. israelensis on cultured cells from aedes aegypti l].the effects of bacillus thuringiensis var. israelensis delta-endotoxin were investigated on a cell culture of aedes aegypti with the electron microscope. the ultrastructural changes following intoxication were: disintegration of endoplasmic reticula by the formation of spherical structures; condensation and then swelling of mitochondria with disappearance of internal cristae; frequent dilatation of the perinuclear space; cellular lysis. the action of a metabolic inhibitor, 2,6-dibromophenol (2,6 ...19836638755
relative toxicity of organic solvents to aedes aegypti larvae. 19836631047
monoclonal antibodies against surface determinants on gametes of plasmodium gallinaceum block transmission of malaria parasites to mosquitoes.monoclonal antibodies (mab) against gametes of the chicken malaria plasmodium gallinaceum have been derived. all reacted with the surface of extracellular gametes of the parasite in immunofluorescent antibody reactions and all agglutinated both male and female gametes. in the absence of active complement one mu isotype mab, la 1-d5, mediated at least 95% suppression of infectivity of the parasites to aedes aegypti mosquitoes. individually, mab of the gamma 1 or gamma 2a isotypes mediated only sl ...19836631012
[new regioselective synthesis of racemic 7,11-dimethyloctadecane--an ovipositon attractant for the yellow fever mosquito (aedes aegypti)]. 19836628194
[diseases transmitted by mosquitoes and urbanization. examples of urban vectors of dengue and filariasis].once located in time and place the beginning of urbanization process, the mechanisms of adaptation to urban ecosystem and of dissemination by human conveyances are studied for two domestic mosquitoes: aedes aegypti and culex pipiens fatigans (= quinque-fasciatus). then epidemiological consequences are discussed. ae. aegypti pullulation constitutes a potential risk of urban yellow fever outbreak and the main factor of dengue haemorrhagic fever appearance; the increase of c. p. fatigans population ...19836627544
the problems of aedes aegypti control in the americas. 19836626807
toxicity of bacillus thuringiensis subsp. israelensis to adult aedes aegypti mosquitoes.adult female aedes aegypti mosquitoes were killed by the parasporal crystals of bacillus thuringiensis subsp. israelensis (onr-60a) when the crystals were introduced into the insect midgut as an enema. the 50% lethal dose for intact parasporal crystals was 0.21 microgram/mg of mosquito (wet weight), and for solubilized crystals the 50% lethal dose was 0.04 microgram/mg. these values were compared with 50% lethal concentrations in a free-feeding larval mosquito bioassay of 0.018 and 1.28 microgra ...19836625566
transovarial transmission of dengue viruses by mosquitoes: aedes albopictus and aedes aegypti.transovarial transmission of all four dengue serotypes was demonstrated in aedes albopictus mosquitoes. the rates of such transmission varied with the serotype and strain of virus. in general, the highest rates were observed with strains of dengue type 1 and the lowest with dengue type 3. surprisingly, despite the use of viral strains of the four dengue serotypes which gave the highest rates with ae. albopictus, transovarial transmission was observed in aedes aegypti only with dengue type 1, and ...19836625066
breeding of aedes aegypti in closed septic tanks. 19836618537
development of dirofilaria repens in aedes aegypti reared in contrasting habitat.the susceptibility of dirofilaria repens infection in aedes aegypti adults reared in hay-infusion and tap-water during their larval stages was tested. ae. aegypti reared in hay-infusion ingested a bigger amount of blood than those reared in tap-water but harboured fewer d. repens larvae (developing and arrested). the difference in the susceptibility of mosquitoes from the two treatments was correlated with the difference in the robustness of the mosquitoes.19836612416
the regulation of preimaginal populations of aedes aegypti (l.) (diptera: culicidae) on the kenya coast. ii. food as a main regulatory factor.experiments were carried out in a village on the kenya coast to investigate observed differences in pupal productivity of aedes aegypti breeding in indoor pots. daily addition of maize gruel, the staple food of villagers, to pots of high and low mosquito production promoted an increase of pupal numbers, although daily introduction of first-instar larvae did not significantly affect pupal production. differences in pupal productivity were not related to differences in the numbers of eggs laid in ...19846547039
[laboratory study of the transmission of dengue virus by aedes aegypti and aedes albopictus]. 19846543740
natural transovarial transmission of dengue 4 virus in aedes aegypti in trinidad.the isolation of dengue 4 virus from adult aedes aegypti, reared from eggs collected in nature, is reported for the first time. from the locality where the isolate was made, 25 pools consisting of 1,848 ae. aegypti reared from eggs were processed. in this study, 10 different localities were sampled and a total of 10,957 ae. aegypti adults, collected as eggs or larvae in nature, were processed for virus isolation. from a total of 158 mosquito pools tested, one recovery of dengue 4 virus was made. ...19846542323
hormonal regulation of ovarian ecdysteroid production in the autogenous mosquito, aedes atropalpus.the effects of juvenile hormone (jh) and egg development neurosecretory hormone (ednh) on ovarian ecdysteroid production during vitellogenesis in the autogenous mosquito, aedes atropalpus, were investigated using in vitro techniques coupled with radioimmunoassay (ria) and high-pressure liquid chromatography (hplc). normal females were characterized by quantitative, qualitative, and temporal patterns of in vitro ovarian ecdysteroid production. females decapitated at emergence showed little ovaria ...19846541607
infectivity of a venezuelan strain of metarhizium anisopliae to aedes aegypti larvae. 19846539354
amino acids and peptides. xi. synthesis of attractant and repellent peptides for aedes aegypti and blattella germanica. 19846532560
comparative development of brugia malayi in susceptible and refractory genotypes of aedes aegypti. 19846527177
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