Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the laboratory mouse model for screening topical mosquito repellents. | eight commercial repellents were tested against aedes aegypti 0 and 4 h after application in serial dilution to volunteers and laboratory mice. results were analyzed by multiple regression of percentage of biting (probit scale) on dose (logarithmic scale) and time. empirical correction terms for conversion of values obtained in tests on mice to values expected in tests on human volunteers were calculated from data obtained on 4 repellents and evaluated with data obtained on 4 others. corrected v ... | 1994 | 7707065 |
| functional expression of insecticide-resistant gaba receptors from the mosquito aedes aegypti. | we are interested in cloning insecticide resistance genes from vector mosquitos for use as selectable markers in their genetic transformation. as a first step towards this goal, we here report the functional homomultimeric expression of a gamma-aminobutyric acid (gaba) receptor subunit gene, resistance to dieldrin (rdl), from the yellow fever mosquito aedes aegypti in baculovirus-infected insect cell lines. replacement of alanine296 with a serine leads to approximately 100-fold insensitivity to ... | 1994 | 7704314 |
| dopachrome conversion activity in aedes aegypti: significance during melanotic encapsulation of parasites and cuticular tanning. | phenol oxidase (po) and dopachrome conversion enzyme (dce) were partially purified from aedes aegypti larvae by ammonium sulfate fractionation. po from a. aegypti functions in the hydroxylation of monophenols (e.g., tyrosine and tyramine) to their related o-diphenols, and the oxidation of o-diphenols (e.g., l-dopa, dopamine, n-acetyldopamine) to their respective o-quinones. partially purified dce showed high specificity toward dopachrome generated from dopa with the l-configuration. the combined ... | 1994 | 7703986 |
| ovariolar 'basal body' development and physiological age of the mosquito aedes aegypti. | dissection of the ovaries of the mosquito aedes aegypti (diptera: culicidae) revealed, in each ovariole, a group of seven to nine specialized epithelial cells in a region of the calyx wall that is enclosed by the end of the ovariolar sheath. this group of cells is termed the basal body. during ovulation, the mature oocyte passes from the ovariole into the calyx lumen through the basal body. subsequently, granulation occurs in the basal body cells. the granular basal bodies differ from all previo ... | 1995 | 7696694 |
| proportions of eggs laid by aedes aegypti on different substrates within an ovitrap in trinidad, west indies. | ovitraps were exposed outdoors during wet and dry seasons in trinidad to determine the proportions of eggs laid on three substrates: the widely-used hardboard 'paddle' (p), the water surface (w) and the inside surface of the ovitrap wall (s) in three types of blackened ovitrap: round, glass (rg), round, plastic (rp) and rectangular, plastic (sp). of the 3556 eggs obtained, most (86.4%) were laid during the wet season when statistically significant differences were detected between substrates but ... | 1995 | 7696690 |
| blood-feeding behavior of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if infection of aedes aegypti with dengue-2 virus affects the ability of the mosquito to efficiently locate and imbibe blood from an uninfected host. previous studies suggest that some parasites manipulate their arthropod host to increase the probability of transmission by interfering with blood-feeding efficiency. we found no evidence that dengue-2 virus infection by intrathoracic inoculation impaired the blood-feeding efficiency of ae. aegypti. we speculate ... | 1995 | 7694963 |
| ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral rna from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction. | a rapid, simple and efficient single-tube procedure is described for the isolation of dengue virus rna from small amount of serum (10 microliters) followed by a reverse transcriptase-polymerase chain reaction (rt-pcr). recovery of rna is based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate in the presence of silica. the silica rt-pcr can be completed within 5 hours starting from rna extraction to agarose gel electrophoresis. all of the 63 dengue-3 culture-positive ... | 1993 | 7689635 |
| extracts of mosquito salivary gland inhibit tumour necrosis factor alpha release from mast cells. | extracts of salivary glands of the yellow fever mosquito aedes aegypti inhibit tumour cell-stimulated release of tumour necrosis factor alpha (tnf alpha) from rat mast cells, but do not inhibit antigen-induced histamine secretion. this inhibitory activity for tnf alpha is found in salivary glands of female but not in male mosquitoes. this inhibition is not mediated by bacterial contamination (lps), by calcitonin gene related peptide (cgrp), nerve growth factor (ngf), epidermal growth factor (egf ... | 1993 | 7679483 |
| community participation in dengue prevention and control: a survey of knowledge, attitudes, and practice in trinidad and tobago. | the priorities of public health planners are often at variance with the community's own environmental sanitation priorities and perspectives. public opinion about individual, collective, and governmental responsibilities in addressing these issues and priorities is of particular importance when designing community-based programs. in a study conducted in trinidad and tobago on knowledge, attitudes, and practice regarding dengue, its prevention and control, a high level of awareness about dengue a ... | 1995 | 7677210 |
| electrophysiological responses of receptor neurons in mosquito maxillary palp sensilla to carbon dioxide. | sensilla basiconica on the maxillary palps of female aedes aegypti contain a receptor neuron which produces a phasic-tonic pattern of action potential response to low concentrations (150-300 ppm) of carbon dioxide (co2), a stimulus known to be involved with host seeking behavior. these receptor neurons respond reliably to small increments in co2 concentration (e.g., 50 ppm). we were particularly interested in evaluating the possibility that the sensitivity to step increases in co2 concentration ... | 1995 | 7674195 |
| hirsutellin a, a toxic protein produced in vitro by hirsutella thompsonii. | a toxic protein, hirsutellin a, has been purified from the mite fungal pathogen, hirsutella thompsonii, using ammonium sulphate precipitation, ion exchange chromatography and gel filtration on bio-gel p-10. the protein has been characterized as a monomer with a molecular mass of 15 kda and an isoelectric point of 10.5. the amino acid composition and the n-terminal sequence of hirsutellin a (34 amino acids) have been determined. from these results, the toxin appears to be distinct from other know ... | 1995 | 7670635 |
| larvicidal potential of five philippine plants against aedes aegypti (linnaeus) and culex quinquefasciatus (say). | five species of philippine plants, reported in the literature to have insecticidal properties, were selected for investigation, namely: anona squamosa ("atis" or sugar apple), eucalyptus globulus ("bagras" or olive gum eucalyptus), lansium domesticum ("lansones"), azadirachta indica ("neem") and codiaeum variegatum ("san francisco" or croton). these were screened and assayed for their larvicidal potential against aedes aedes aegypti (linnaeus) and culex quinquefasciatus (say) by exposing 3rd-4th ... | 1994 | 7667727 |
| population interaction of toxorhynchites splendens and aedes aegypti (diptera: culicidae) in the laboratory. | population interaction of toxorhynchites splendens and aedes aegypti in relation to the complexity of the breeding habitats and their initial number was studied in the laboratory. the predator and the prey were introduced in different ratios in the colony cages (1 m3) with different oviposition structures. predator-prey interaction lasted for 5-9 weeks without structural complexity of the oviposition containers. when there was a structural complexity, their interaction lasted for 18 weeks. durin ... | 1994 | 7667726 |
| isolation and characterization of mosquito ferritin and cloning of a cdna that encodes one subunit. | ferritin, an iron storage protein, was isolated from larvae and pupae of aedes aegypti grown in an iron-rich medium. mosquito ferritin is a high molecular weight protein composed of several different, relatively small, subunits. subunits of molecular mass 24, 26, and 28 kda are equally abundant, while that of 30 kda is present only in small amounts. the n-terminal sequence of the 24 and 26 kda subunits are identical for the first 30 amino acids, while that of the 28 kda subunit differs. studies ... | 1995 | 7655055 |
| spread of aedes albopictus and decline of ae. aegypti (diptera: culicidae) in florida. | waste tires and other types of artificial containers were sampled for immature aedes to monitor changes in the occurrence of aedes aegypti (l.) and aedes albopictus (skuse) in florida. the initial invasion and spread of ae. albopictus in florida occurred in the northern part of florida. throughout this region, major declines in the abundance of ae. aegypti have been associated with the expansion of ae. albopictus in both urban and rural areas. generally, the same results have occurred in central ... | 1995 | 7650719 |
| edhazardia aedis (microspora: culicosporidae) effects on the reproductive capacity of aedes aegypti (diptera: culicidae). | the influence of the microsporidium edhazardia aedis (kudo) on the survival and reproduction of its mosquito host, aedes aegypti (l.), was studied in the laboratory. survival, fecundity, egg hatch, and percentage of emergence for 4 gonotrophic cycles were compared for control and infected mosquitoes. control females oviposited an average of 123.1 eggs over 4 gonotrophic cycles, 86.1% of which hatched, whereas infected females laid an average of 38.0 eggs with a 69.3% hatch. emergence in progeny ... | 1995 | 7650718 |
| age and body size effects on blood meal size and multiple blood feeding by aedes aegypti (diptera: culicidae). | ten-day-old, parous aedes aegypti (l.) had a higher threshold of blood meal size for the inhibition of continued host-seeking than did 5 d-old, nulliparous females of the same size. older females of a smaller-bodied cohort had a higher threshold of blood meal size for the initiation of egg development than did younger females. in contrast, older larger-sized females had a lower blood meal-size threshold for egg development. the blood of paired avian hosts was marked with rubidium and cesium to s ... | 1995 | 7650708 |
| molecular cloning of a major cockroach (blattella germanica) allergen, bla g 2. sequence homology to the aspartic proteases. | inhalation of allergens produced by the german cockroach (blattella germanica) elicits ige antibody formation and the development of asthma in genetically predisposed individuals. we compared the allergenic importance of two cockroach (cr) allergens, bla g 1 and bla g 2, and determined the complete amino acid sequence of the major 36-kda allergen, bla g2. a survey of 106 sera from cr allergic patients showed the prevalence of ige antibodies to bla g 1 and bla g 2 to be 30.2% and 57.6%, respectiv ... | 1995 | 7642642 |
| dengue in french guiana, 1965-1993. | while it seems likely that dengue fever (df) has existed in french guiana for at least one century, data on outbreaks are sketchy before temporary eradication of the dengue vector mosquito aedes aegypti and its reestablishment in the early 1960s. dengue cases were serologically confirmed for the first time in 1965, and since then dengue epidemics have occurred at two to six year intervals, the most important occurring in 1968-1969, 1970, 1972, 1976, 1982, 1986, and 1992. three of the four dengue ... | 1995 | 7640693 |
| laboratory evaluation of an ovipositional trap for mosquito adults aedes aegypti (l.) (diptera: culicidae). | a trap was designed, fabricated and evaluated for trapping and killing ovipositing females of the yellow fever mosquito aedes. aegypti. water in which larval stages had previously dwelt for 48 hrs. formed the attractant source for ovipositing mosquito females. the trap was evaluated under different experimental conditions. single choice and double choice experiments were performed with or without glue applied to inner surfaces, to determine trap efficacy. effect of population size and alteration ... | 1995 | 7636150 |
| cloning, sequencing and functional expression of an acetylcholinesterase gene from the yellow fever mosquito aedes aegypti. | a degenerate pcr strategy was used to isolate a fragment of the acetylcholinesterase gene (ace) homolog from aedes aegypti and screen for a cdna clone containing the complete open reading frame of the gene. the predicted amino acid sequence of the aedes gene shares 64% identity with ace from drosophila and 87% identity with the acetylcholinesterase gene from another mosquito species anopheles stephensi. high levels of expression of the aedes gene were achieved by infection of sf21 cells with a r ... | 1995 | 7635199 |
| insect immunity: isolation of three novel inducible antibacterial defensins from the vector mosquito, aedes aegypti. | the injection of escherichia coli and micrococcus luteus into the hemocoel of aedes aegypti induces a potent antibacterial activity in the hemolymph. we have purified and fully characterized three 40-residue antibacterial peptides from the hemolymph of bacteria-challenged mosquitoes that are absent in naive mosquitoes. the peptides are potently active against gram-positive bacteria and against one of the gram-negative bacteria that were tested. the amino acid sequences clearly show that the thre ... | 1995 | 7633471 |
| mosquito vitellogenin receptor: purification, developmental and biochemical characterization. | vitellogenin receptors (vgrs) play a critical role in egg development of oviparous animals by mediating endocytosis of the major yolk protein precursor, vitellogenin. a modification of the method for extracting the mosquito (aedes aegypti) vgr from ovary membranes resulted in an 11-fold higher yield and 56-fold increase in relative purity of the vgr, in turn permitting purification, antibody production, and microsequencing. a kd of 15 nm was estimated from binding assays for the enriched vgr, in ... | 1995 | 7633468 |
| field evaluation of alphacypermethrin and lambda-cyhalothrin against aedes aegypti and aedes albopictus in malaysia. | alphacypermethrin (fendona) and lambda-cyhalothrin (icon) were evaluated against sentinel bloodfed adults and 4th-instar larvae of aedes aegypti in a housing estate in malaysia. the impact of both pyrethroids on field populations of ae. aegypti and aedes albopictus were monitored weekly using oviposition trap samples. both alphacypermethrin and lambda-cyhalothrin showed adulticidal and larvicidal effects. | 1995 | 7616191 |
| an immunological factor that affects anopheles gambiae survival. | high titers of antibodies against anopheles gambiae midguts were produced in new zealand rabbits to identify midgut targets for an antimosquito vaccine. the serum from one of 8 rabbits (designated r2b6) killed 71.6% (abbott's adjusted % mortality) of an. gambiae within 7 days. mosquitoes ingesting r2b6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. anopheles stephensi and anopheles arabiensis were also killed when ingesting r2b6 s ... | 1995 | 7616189 |
| relationship between cold hardiness and supercooling point in aedes albopictus eggs. | for many insects, low temperatures are not lethal unless their tissues freeze. to determine whether freezing is the cause of low temperature mortality of aedes albopictus eggs, we measured their lower lethal temperature, the temperature that causes 50% mortality in a population, and supercooling point, the temperature at which the tissues freeze. tropical and temperate ae. albopictus eggs were tested under different diapause and cold acclimation conditions. temperate ae. albopictus eggs had a lo ... | 1995 | 7616187 |
| insect repellent formulations of n,n-diethyl-m-toluamide (deet) in a liposphere system: efficacy and skin uptake. | novel formulations for a deet in liposphere microdispersion in the form of lotion were prepared from natural solid triglycerides and phospholipids dispersed in buffer solution. the formulations containing 6.5, 10, and 20% deet were effective as a repellent against the common aggressive biting mosquitoes, aedes aegypti and anopheles stephensi, for up to 6 h. the acute dermal absorption of the 10% loaded formulation was conducted in rabbits using 14c-labeled deet. 14c-labeled deet, 10% in alcohol ... | 1995 | 7616186 |
| dna sequence and site of mutation of the gaba receptor of cyclodiene-resistant red flour beetle, tribolium castaneum. | using polymerase chain reactions (pcr) on cdna, the dna sequence of a membrane spanning region of a gaba receptor of the red flour beetle, tribolium castaneum was identified. the deduced amino acid sequence indicates that its basic structure is similar to the gaba receptor of rdl type subunits of drosophila melanogaster and of blattella germanica. particularly conserved are m1, m2 and m3 segments. within this 146 amino acid stretch, the gaba receptor from the red flour beetle differed from corre ... | 1995 | 7613764 |
| risk factors for dengue infection during an outbreak in yanes, puerto rico in 1991. | in november 1991, during a five-month dengue outbreak, we performed epidemiologic and serologic surveys linked to an earlier entomologic study in a community of 425 houses in yanes (florida), puerto rico. we obtained a household response rate of 95% (98 of 103) and blood samples from 84% (345 of 410) of the participants. dengue incidence, as volunteered by the respondents, was 5% (21 of 410), but serologic diagnosis (immunoglobulin m and igg-enzyme-linked immunosorbent assays [elisa]) indicated ... | 1995 | 7611553 |
| gene for cytochrome c oxidase subunit ii in the mitochondrial dna of culex quinquefasciatus and aedes aegypti (diptera: culicidae). | the cytochrome c oxidase subunit ii (coii) gene between transfer rna for leu and lys in the mitochondrial dna of culex quinquefasciatus say and aedes aegypti (l.) was amplified by the polymerase chain reaction (pcr) technique. both the gene order and direction of transcription were identical to anopheles and drosophila. nucleotide sequences of the pcr-amplified coii genes in these two mosquitoes exhibited 88% homology, and the frequency of transition was very close to that of transversion. the h ... | 1995 | 7608924 |
| aedes albopictus and other aedes (stegomyia) species in fiji. | during an assessment of the dengue situation in fiji in early 1992, a house-to-house survey of container-breeding aedes was made. discarded tires and water drums were identified as key breeding sites for the 4 potential dengue vectors: aedes aegypti, aedes albopictus, aedes pseudoscutellaris, and aedes polynesiensis. aedes albopictus were detected on viti levu, vanua levu, and on taveuni. examination of early records and of used tire importation suggests entry into fiji after july 1985 but well ... | 1995 | 7595450 |
| impact of deltamethrin-impregnated bednets on biting rates of mosquitoes in zaire. | in a rural area of zaire, the whole population of a village was protected by deltamethrin-impregnated mosquitoes bednets. a similar village was observed as a control. biting rates for mosquitoes were recorded in both villages. the principal man-biting species were mansonia africana, mansonia uniformis, and aedes aegypti. in the village protected by the impregnated mosquito bednets, the number of mansonia bites was reduced 96% indoors and at a lesser rate outdoors. biting rates of ae. aegypti dro ... | 1995 | 7595444 |
| laboratory evaluation of a new repellent camouflage face paint. | a combined formulation of the u.s. army's camouflage face paints and the new extended duration topical insect/arthropod repellent was tested on human volunteers to determine repellency and duration of protection (2-12 h) against aedes aegypti and anopheles stephensi. under laboratory conditions (27 degrees c and 80% rh), the face paint/repellent formulation provided > or = 95% repellency or better for up to 6 h for ae. aegypti and 8 h for an. stephensi. an analysis of variance showed no signific ... | 1995 | 7595441 |
| control of mosquito breeding using wood scrapings treated with neem oil. | wood scrapings were given shape of a ball and soaked in 5, 10 and 20% neem (azadirachta indica) oil diluted in acetone. control of anopheles stephensi and aedes aegypti breeding in water storage overhead tanks (ohts) with the application of these balls was achieved for 45 days. two balls soaked in 5% neem oil produced the best results among other concentrations tested. | 1995 | 7589730 |
| partial purification of plasma phenoloxidase of the yellow fever mosquito aedes aegypti l. (diptera: culicidae). | a nitrocellulose-based assay was developed using a dot-blot apparatus to detect phenoloxidase activity in column fractions. using this assay, plasma phenoloxidase was partially purified from aedes aegypti larvae using hydrophobic interaction chromatography, gel filtration, and ion-exchange chromatography. the molecular weight (m(r)) native enzyme was 130,000, and it contained subunits of 76,000, 62,000, and 58,000. two phenoloxidase peaks were observed by ion exchange chromatography, and these f ... | 1995 | 7584838 |
| post-inoculation changes in enzyme activity of aedes aegypti infected with chikungunya virus. | levels of acetylcholinesterase, non-specific esterases, glutathione-s-transferase and glucose-6-phosphate dehydrogenase in aedes aegypti (l.) mosquitoes inoculated intrathoracally with chikungunya virus were elevated, as compared to uninoculated control insects. a number of these enzymes are important in the insects defence mechanism against xenobiotics, such as pesticides. malathion bioassays indicated a reduction in the susceptibility of experimentally injected insects with virus or virus-free ... | 1995 | 7572467 |
| aminopropeptide of human procollagen type i: a marker for the identification of blood from children in the mosquito blood meal. | a capture enzyme-linked immunosorbent assay (elisa) to distinguish between blood from children and adults in the mosquito blood meal was examined using the alpha 1 chain of the aminopropeptide of human procollagen type i (pinp) as antigenic marker. rabbit anti-human pinp (alpha 1) antibody was used as catching antibody, and either normal serum from 288 african and 58 caucasian children and adults, or blood meals from 93 fed aedes aegypti, were examined. pinp in excess of 40 optical density units ... | 1995 | 7570897 |
| full sequence and characterization of two insect defensins: immune peptides from the mosquito aedes aegypti. | we report the complete amino acid sequence and biological activity of two immune peptides, from the yellow fever mosquito aedes aegypti, that are induced in response to infection. both peptides display biological activity against the gram positive microbe micrococcus luteus and substantial sequence homology to insect defensins, small heat-stable, antibiotic peptides previously described from several non-vector insects. these mosquito peptides, designated ae. aegypti defensins a and b, are isofor ... | 1995 | 7568275 |
| venezuelan equine encephalitis--colombia, 1995. | an outbreak of venezuelan equine encephalitis (vee) that began in northwestern venezuela in april 1995 has spread westward to the guajira peninsula and to colombia (figure 1), resulting in an estimated minimum of 13,000 cases in humans and an undetermined number of equine deaths. governments of both countries have initiated efforts to control the spread of this outbreak by quarantining and vaccinating equines and applying insecticides. this report summarizes the ongoing investigation of the outb ... | 1995 | 7565549 |
| effect of ethylene bis isobutyl xanthate isolated from a marine green alga dictyosphaeria favulosa on mosquito aedes aegypti. | 1995 | 7558202 | |
| mosquito sensitivity to a scorpion neurotoxin expressed using an infectious sindbis virus vector. | the scorpion, androctonus australis hector, produces an insect-specific toxin (aahit) encoded by the scotox gene. to assess the toxicity of aahit for mosquitoes, we have taken a novel approach to express the scotox gene in vivo. we have engineered a double subgenomic sindbis (dssin) virus that contains the scotox gene in the viral genome and intrathoracically inoculated the virus (te/3'2j/scotox) into mosquitoes (aedes aegypti, ae. triseriatus and culex pipiens), houseflies (musca domestica) and ... | 1995 | 7551198 |
| analysis of a vitellogenin gene of the mosquito, aedes aegypti and comparisons to vitellogenins from other organisms. | a genomic clone of the aedes aegypti vitellogenin a1 gene was sequenced including 2015 bp of 5' untranscribed sequence, 6369 bp of open reading frame interrupted by two introns, and a short 3' untranslated region. primer extension was used to identify the transcription initiation site. the amino termini of the large and small subunits were located by n-terminal sequencing of vitellin purified from eggs. the length of the signal sequence and the position of the cleavage site between the two subun ... | 1995 | 7550249 |
| improved medium for aedes aegypti tissue culture. | 1995 | 7548955 | |
| resistance in some caribbean populations of aedes aegypti to several insecticides. | thirty-four strains of aedes aegypti larvae from 17 caribbean countries were bioassayed for sensitivity to temephos, malathion, fenitrothion, fenthion, and chlorpyrifos. there were fairly high levels of resistance in tortola (10-12-fold resistance) and antigua (6-9-fold resistance) strains to temephos and to fenthion (tortola, 7-10-fold; antigua, 6-10-fold resistance). most other strains showed some resistance to malathion, fenitrothion, and chlorpyrifos, but only moderate levels. adult populati ... | 1995 | 7542312 |
| hemocyte alterations during melanotic encapsulation of brugia malayi in the mosquito armigeres subalbatus. | the involvement of hemocytes in melanotic encapsulation reactions against brugia malayi was assessed in armigeres subalbatus. hemocyte populations, epitope changes, phenol oxidase (po) activity, and the presence of an 84-kda polypeptide were investigated in mosquitoes exposed to a b. malayi-infective bloodmeal (= immune-activated), in mosquitoes given a noninfective bloodmeal (= controls), in nonbloodfed mosquitoes (= naive), or in some combination of these. total hemocyte populations in immune- ... | 1995 | 7535848 |
| unique specificity of in vitro inhibition of mosquito midgut trypsin-like activity correlates with in vivo inhibition of malaria parasite infectivity. | synchrony in the egress of plasmodium ookinetes from the food bolus and enzymatic digestion of the blood meal in the mosquito midgut suggests that digestive enzymes play a role in the successful transmission of malaria parasites. previously, we found that parasite-produced chitinase is essential for parasite transmission and can be activated by mosquito midgut protease. to determine the suitability of developing a transmission-blocking vaccine directed against mosquito trypsin-like enzyme(s), ae ... | 1995 | 7534722 |
| identification of damaged adult female specimens of aedes albopictus and aedes aegypti in the new world. | two introduced species of aedes (stegomyia), aedes albopictus and aedes aegypti, occur in the new world. three characters, easily and simultaneously observed from an anteroventral view, allow for rapid and reliable specific identification of most specimens that lack the characteristic scutal scale patterns. these 3 characters are the presence or absence of pale scales on the clypeus; the presence or absence of a narrow, median line of pale scales on the anterior face of the midfemora; and the pa ... | 1994 | 7528784 |
| studies on the effect of translocation of chemical insecticides in eicchornia host plant on mansonia mosquitos--a potential control method. | a novel method for the control of mansonia larvae was developed and tested. in this method, foliar absorption and translocation of a chemical insecticide, monocrotophos, a known systemic insecticide was studied in the eicchornia plant. acetone solution of the insecticide was painted onto leaves of the plant. at daily intervals, stems were severed and divided into equal sections which were introduced into bowls. larvae of aedes aegypti were tested for the presence of monocrotophos. it was found t ... | 1993 | 7526473 |
| dietary control of late trypsin gene transcription in aedes aegypti. | in aedes aegypti the levels of midgut trypsin activity after feeding are directly proportional to the protein concentration in the meal. the mechanisms of this up-regulatory event were investigated by analyzing the expression of the late trypsin gene under different dietary conditions. transcription of the gene was dependent on both the quality and quantity of protein in the meal. as measured by northern blot analysis, the levels of late trypsin gene expression increased up to 100-fold 24 h afte ... | 1994 | 7519098 |
| the salivary glands of the vector mosquito, aedes aegypti, express a novel member of the amylase gene family. | several cdna clones with similarity to alpha-amylases have been characterized from a library made from adult female salivary gland rna isolated from the vector mosquito, aedes aegypti. the corresponding gene, designated amylase i (amy i), is expressed specifically in the proximal-lateral lobes of the adult female salivary gland, a pattern overlapping that of another gene, mal i, involved in carbohydrate metabolism. the deduced amino acid sequence of amy i indicates that this gene encodes a prote ... | 1993 | 7505701 |
| plasmodium gallinaceum: mosquito peritrophic matrix and the parasite-vector compatibility. | transmission of malaria parasites occurs by relatively few species of mosquitoes. one proposed mechanism of refractoriness is an inability of certain plasmodium spp. to cross the peritrophic matrix (pm) in the midgut of an incompatible mosquito. we have tested this hypothesis by studying sporogonic development of plasmodium gallinaceum in susceptible (aedes aegypti and anopheles gambiae g3) and refractory (anopheles stephensi) mosquito species in the presence and absence of the pm. in the presen ... | 1995 | 7498435 |
| aedes aegypti: a quantitative trait locus (qtl) influencing filarial worm intensity is linked to qtl for susceptibility to other mosquito-borne pathogens. | because intensity of infection was significantly increased in a substrain of aedes aegypti selected for susceptibility to the filarial worm, brugia malayi, experiments were designed to assess numbers of microfilariae (mf) ingested and midgut penetration by mf in this susceptible substrain as compared to a refractory substrain selected from the same parental stock. refractory mosquitoes ingested significantly fewer mf than susceptible mosquitoes and significantly fewer numbers of mf penetrated th ... | 1995 | 7498432 |
| plasmodium gallinaceum: sporozoite invasion of aedes aegypti salivary glands is inhibited by anti-gland antibodies and by lectins. | there is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. we are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. we used an in vivo bioassay for sporozoite invasion of salivary glands. in this assay, purified sporozoites from mature oocytes of plasmodium gallinaceum were injected into aedes aegypti mosquitoes and salivary glands were dissected at different time points after inj ... | 1995 | 7498430 |
| a factor xa-directed anticoagulant from the salivary glands of the yellow fever mosquito aedes aegypti. | salivary gland extracts from the yellow fever mosquito aedes aegypti contain a factor xa-specific anticoagulant detectable in vitro by clotting assays. the anticoagulant is found only in female salivary glands, which is consistent with its role in bloodfeeding. extracts of adult female glands significantly delay both the prothrombin time and the activated partial thromboplastin time, but have no effect on the thrombin clotting time. the anticoagulant has been shown to result from the specific in ... | 1995 | 7498429 |
| two genes encoding midgut-specific maltase-like polypeptides from anopheles gambiae. | full-length cdna clones of two genes have been isolated from the african malaria vector mosquito, anopheles gambiae. these genes, designated agm1 and agm2, encode maltase-like polypeptides of 498 and 599 residues, respectively. deduced amino acid sequences contain a putative signal peptide sequence and four potential glycosylation sites. agm1 and agm2 show highest similarities to the mal1 gene from aedes aegypti and three clustered maltase genes from drosophila melanogaster. both genes are locat ... | 1995 | 7498424 |
| the apyrase gene of the vector mosquito, aedes aegypti, is expressed specifically in the adult female salivary glands. | the yellow fever mosquito, aedes aegypti, expresses a gene, apyrase (apy), that encodes an atp-diphosphohydrolase. the product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. apy gene expression is limited to the cells of the distal-lateral and medial lobes of the adult female salivary glands. apyrase protein levels, detectable by antibodies, peak in the salivary glands about 4 days after adult emergence and remain high after a blood ... | 1995 | 7498420 |
| non-linear dynamics of neurochemical modulation of mosquito oviduct and hindgut contractions. | microphysiological techniques and computer-based methods of data acquisition and analysis were used to investigate the dynamics of neurochemical action on spontaneous contractions of continuously perfused oviducts and hindguts isolated from female mosquitoes (aedes aegypti). proctolin, leucomyosuppressin (lms), serotonin and octopamine, which are known to modulate insect muscle contractions, and a mosquito neuropeptide, aedes head peptide i (aea-hp-i), which inhibits host-seeking behavior, were ... | 1995 | 7490571 |
| isolation and identification of novel toxins from a new mosquitocidal isolate from malaysia, bacillus thuringiensis subsp. jegathesan. | a new mosquitocidal bacillus thuringiensis subsp., jegathesan, has recently been isolated from malaysia. parasporal crystal inclusions were purified from this strain and bioassayed against fourth-instar larvae of culex quinquefasciatus, aedes aegypti, aedes togoi, aedes albopictus, anopheles maculatus, and mansonia uniformis. the 50% lethal concentration of crystal inclusions for each species was 0.34, 8.08, 0.34, 17.59, 3.91, and 120 ng/ml, respectively. these values show that parasporal inclus ... | 1995 | 7487029 |
| a simulation model of the epidemiology of urban dengue fever: literature analysis, model development, preliminary validation, and samples of simulation results. | we have developed a pair of stochastic simulation models that describe the daily dynamics of dengue virus transmission in the urban environment. our goal has been to construct comprehensive models that take into account the majority of factors known to influence dengue epidemiology. the models have an orientation toward site-specific data and are designed to be used by operational programs as well as researchers. the first model, the container-inhabiting mosquito simulation model (cimsim), a wea ... | 1995 | 7485707 |
| aedes aegypti control in the lao people's democratic republic, with reference to copepods. | an evaluation of the lao aedes aegypti control program and of the predatory abilities of copepods from vientiane, lao people's democratic republic was undertaken before a field release of copepods in thongkankam village, vientiane. copepods were transported to australia for evaluation of predatory abilities and their survival under various nutrient and ph conditions. mesocyclops guangxiensis was chosen for release over m. aspericornis due to its higher reproduction rate and its ability to surviv ... | 1995 | 7485682 |
| feeding the mosquito aedes aegypti with tmof and its analogs; effect on trypsin biosynthesis and egg development. | female aedes aegypti that were given a blood meal by enema deposited yolk in their oocytes and synthesized trypsinlike enzymes in their midgut. when females were given an enema of aea-tmof (trypsin modulating oostatic factor) (nh2-ydpapppppp-cooh) and blood both egg development and trypsin biosynthesis were inhibited. similar results were observed if tmof was mixed with the blood meal and fed to female mosquitoes through a membrane. renin inhibitor (nh2-phpfhffvyk-cooh) or poly proline given by ... | 1995 | 7480877 |
| effects of two insect growth regulators on ecdysteroid production in aedes aegypti (diptera: culicidae). | diflubenzuron and oms 2017 are insect growth regulators that affect larval to adult development in aedes aegypti (l.) by altering ecdysis. when larvae were exposed to sublethal concentrations, surviving adults express reduced reproductive potential. in mosquitoes, ecdysteroids are important in larval and adult ovarian development. we applied 30% emergence reduction concentrations (ei30) of oms 2017 and diflubenzuron to 4th-instar ae. aegypti to determine if changes in ecdysteroid production may ... | 1995 | 7473612 |
| the genetic basis of susceptibility to infection with dirofilaria immitis in aedes aegypti. | 1980 | 7458468 | |
| malathion resistance in aedes aegypti of puerto rico induced by selection pressure on larvae. | a high level of tolerance to malathion occurred in aedes aegypti larvae of san juan, puerto rico in 1978. the tolerance was 7-fold when compared with a susceptible strain. malathion selection pressure in the laboratory on this tolerant strain resulted in resistance after only six filial generations. the resistance of the f6 generation as 10-fold when compared with the strain as it was in the field in 1969, and 19-fold when compared with a susceptible laboratory strain. the results, together with ... | 1980 | 7446832 |
| the presence of oviposition attractants of aedes albopictus larval holding water on aedes aegypti. | 1980 | 7444586 | |
| yellow fever in the gambia, 1978--1979: entomological aspects and epidemiological correlations. | an entomological survey was conducted in the gambia in january 1979, during the last phase of a yellow fever (yf) outbreak which began during the previous rainy season. in the dry conditions which prevailed in january, aedes aegypti was the only yf vector present. two yf virus strains were isolated from females of this mosquito species caught in a village of western gambia, where active human cases were documented. the ae. aegypti breeding sites were exclusively of the domestic type. larval indi ... | 1980 | 7435794 |
| yellow fever in the gambia, 1978--1979: epidemiologic aspects with observations on the occurrence of orungo virus infections. | an epidemic of yellow fever (yf) occurred in the gambia between may 1978 and january 1979. retrospective case-finding methods and active surveillance led to the identification of 271 clinically suspected cases. a confirmatory or presumptive laboratory diagnosis was established in 94 cases. the earliest serologically documented case occurred in june 1978, at the extreme east of the gambia. small numbers of cases occurred in august and september. the epidemic peaked in october, and cases continued ... | 1980 | 7435793 |
| a comparison of the number of multiple enzymatic forms in gravid and nongravid aedes aegypti (diptera: culicidae). | 1980 | 7420365 | |
| the urban mosquitoes of suva, fiji: seasonal incidence and evaluation of environmental sanitation and ulv spraying for their control. | larval surveys and oviposition traps were used to monitor urban mosquito populations in two adjacent transects in suva, fiji between may 1978 and august 1979. populations of aedes aegypti and ae. pseudoscutellaris fluctuated seasonally with changes in rainfall, the latter species being most prevalent throughout the year. populations of these two species were highest between december and july and lowest between august and october. larval populations of culex quinquefasciatus did not show a season ... | 1980 | 7411679 |
| infection of aedes aegypti with onchocerca gutturosa. | 1980 | 7410805 | |
| aedes aegypti as a surrogate host for onchocerca spp. | 1980 | 7386718 | |
| the reinvasion of colombia by aedes aegypti: aspects to remember. | 1980 | 7386716 | |
| genetics and the origin of a vector population: aedes aegypti, a case study. | thirty-four population samples representing the worldwide distribution of the mosquito aedes aegypti were analyzed for variation at 19 to 22 enzyme-coding genes. a multivariate discriminant analysis revealed that the genetic differences among populations in six geographic regions and between two subspecies enable one to determine the regional origin of a population. such studies of population genetics may have quite general applicability in studying vector-borne diseases. | 1980 | 7375945 |
| topical mosquito repellents xiii: cyclic analogs of lactic acid. | numerous cyclic analogs of lactic acid were synthesized to ascertain whether they might act as agonists of antagonists of lactic acid, a known attractant for mosquitoes. these compounds were evaluated with an in vitro blood-feeding test system and an in vivo cloth test. two of the compounds in the blood-feeding test system showed biphasic results, acting as attractants at low concentrations and as repellents at higher concentrations. several compounds (iii, v, vii, and x) repelled aedes aegypti ... | 1980 | 7359323 |
| comparative sensitivity of mosquito inoculation and mammalian cell culture for isolation of some arboviruses in indonesia. | the sensitivity of parenteral inoculation of colony reared male aedes aegypti and mammalian cell cultures for isolation of japanese encephalitis virus and dengue virus were compared. the mosquito inoculation technique proved to be more sensitive for the isolation of dengue virus from the sera of febrile patients than did the mammalian cell cultures (vero and bhk21) employed in these studies. mosquito inoculation proved to be no more sensitive that the mammalian system for the isolation of japane ... | 1981 | 7344103 |
| scavenger snail secretion as an ovipositional attractant for aedes aegypti. | 1981 | 7343593 | |
| sequential development changes in microfilariae of subperiodic brugia malayi to infective larvae in susceptible strain of aedes aegypti (macdonald). | 1981 | 7343589 | |
| studies on the breeding habitats and seasonal prevalence of larval population of aedes aegypti (l.) and aedes albopictus (skuse) in dacca city. | an one-year study was conducted on the outdoor breeding habitats and seasonal prevalence of larval populations of aedes aegypti and aedes albopictus in ten different locations of dacca city. nine locations were found infested with larvae of either one or both species during the rainy season (may to october) of the year. a total of 1898 containers/sites were surveyed out of which 86 (4.53%) were infested with either one or both species. out of then different kinds of artificial containers and nat ... | 1980 | 7325925 |
| observations on container-breeding mosquitoes in new orleans, louisiana, with an estimate of the population density of aedes aegypti (l.). | in a survey of containers infested with aedes aegypti (l.) conducted during september and october 1979 in a substandard residential area of new orleans, la, infestation indices were as follows: house--35, breteau (foci)--85, receptacle--69, block--100, and larval density--169. a mean of 48.5 water-holding containers were found per block. more than 50% of these receptacles were narrow-mouthed containers, e.g., drink bottles, that held an average of less than 0.03 mosquito immatures each. after ex ... | 1981 | 7325289 |
| transmission of ross river virus by aedes polynesiensis and aedes aegypti. | laboratory studies were carried out with two geographic strains of aedes polynesiensis and one strain of aedes aegypti to determine whether they could transmit ross river virus (rrv). both species were shown to be good vectors of rrv, but ae. polynesiensis was the most susceptible. ae. polynesiensis represents a new vector for this virus and the epidemiologic implications of rrv spread by both mosquito species are discussed. | 1981 | 7325287 |
| infection of aedes aegypti with brugia pahangi administered by enema: results of quantitative infection and loss of infective larvae during blood feeding. | a technique for infecting mosquitoes with known numbers of brugia pahangi microfilariae by enema is described. virtually all mosquitoes receiving three microfilariae or more by this route contained infective larvae 10 days later. within a range of 1 to 40 microfilariae, numbers of infective larvae recovered (y) were related to the numbers of microfilariae administered (x) by the equation log10y=0.04 + 0.84 log10x. mosquitoes feeding on sugar for up to 20 days did not lose a significant number of ... | 1981 | 7324102 |
| relative activity of bacillus thuringiensis var. kurstaki and b. thuringiensis var. israelensis against larvae of aedes aegypti, culex quinquefasciatus, trichoplusia ni, heliothis zea, and heliothis virescens. | 1981 | 7320315 | |
| evaluation of methoprene (a juvenile hormone) against culex quinquefasciatus, anopheles stephensi and aedes aegypti. | 1981 | 7309181 | |
| aedes aegypti: a sword of damocles over tropical america. | 1981 | 7306736 | |
| laboratory transmission of brugia pahangi by nulliparous aedes aegypti (diptera: culicidae). | 1981 | 7299793 | |
| a model for the mechanism of action of the repellent deet on aedes aegypti (diptera: culicidae). | 1981 | 7299789 | |
| [ph variations in the midgut of aedes aegypti in relation to bacillus thuringiensis var. israelensis (serotype h14) crystal intoxication]. | after feeding aedes aegypti larvae with b. thuringiensis var. israelensis crystals the very alkaline ph (greater than or equal to 10) of the anterior midgut remains stable during the first twenty minutes, in spite of important cytological damage. thereafter, the ph drops to surround the neutrality and the death occurs. these results are discussed according to the knowledge on the action of b. thuringiensis var. israelensis. | 1981 | 7296735 |
| morphogenesis of yellow fever virus in aedes aegypti cultured cells. ii. an ultrastructural study. | the growth and intracytoplasmic development of two yellow fever virus strains (wild and french neurotropic) were studied in aedes aegypti cells (clone c 17). despite a longer period of latency for the vaccine virus, infected cells appeared similar. the cisternae of the rough endoplasmic reticulum (rer) were swollen and formed vesicles which contained the virus. this rer appeared to be the predominant locus of viral synthesis and maturation. cytopathic effect appeared when the cells were filled w ... | 1981 | 7283005 |
| genetic aspects of the susceptibility of aedes aegypti to dirofilaria and brugia. | 1981 | 7268859 | |
| factors affecting syncytial development in aedes pseudoscutellaris cells by dengue viruses. | several factors that were suspected of affecting the development of syncytia in cultured aedes pseudoscutellaris cells inoculated with dengue viruses were studied. the results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 x 10(5) cells/cm2), and low cell passage level (less than 52 passages) favored the development of syncytia. all three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by aedes pseudoscutellaris cell culture ... | 1981 | 7258498 |
| genetic response of aedes aegypti to different environmental temperatures. | three groups of aedes aegypti maintained 8 generations in the laboratory at 20 degrees c, 27 +/- 1 degree c (normal control), and at 35 degrees c, respectively were analyzed for allozyme variability. of the 8 larval loci examined est alpha-1 and lap-2 in generation 8 were still variable, while the other allozymes became uniform. the 35 degrees c population had mean level of heterozygosity of about 6% of individual per locus, less than those observed in the 20 degrees c and the control population ... | 1981 | 7256357 |
| [effect of conditions and duration of aedes aegypti densonucleosis virus storage on its infective properties]. | 1981 | 7253970 | |
| acetylcholinesterase activity in an aedes aegypti cell line. | considerable acetylcholinesterase (ache) activity was detected in an aedes aegypti established cell line. the enzyme is blocked by 10(-6) m eserine sulfate, displays excess substrate inhibition and slowly hydrolyzes butyrylthiocholine. a 2-fold stimulation of ache activity was shown after 2 days exposure to 3 x 10(-7) m beta-ecdysone. ache activity found in the fresh medium is the contribution of the fetal calf serum portion. a direct relationship between levels of serum and the ache activity in ... | 1981 | 7238835 |
| disruption of insect photoreceptor membrane by divalent ions: dissimilar sensitivity of light- and dark-adapted mosquito rhabdomeres. | the conditions that lead to the formation of myelin figures in rhabdomere microvilli were studied in the larval ocelli of the mosquito aedes aegypti. these artifacts can result from the addition of divalent ions, such as ca2+, to primary-aldehyde fixatives, but they form subsequently during postfixation with oso4. in light-adapted ocelli, myelin figures are concentrated at the proximal ends of the microvilli along the cytoplasmic margin of the rhabdomere. the severity of the artifact is proporti ... | 1981 | 7226214 |
| a preliminary survey of aedes aegypti in selangor, peninsular malaysia. | 1980 | 7219273 | |
| steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. | a cultured cell line of the mosquito, aedes aegypti, is sensitive to tunicamycin as expected from the ability of crude membrane preparations to catalyse the formation of n-acetylglucosamine-linked dolichyl pyrophosphate. formation of dolichylphosphomannose was also detected and this reaction was totally insensitive to tunicamycin. incorporation of radioactive mannose into total acid-precipitable glycoproteins was inhibited greater than 90% in whole cells by tunicamycin, while the incorporation o ... | 1981 | 7213699 |
| isolation and characterization of mosquito cell membrane glycoproteins. | plasma membranes have been purified from an established cell line, mos 20a of aedes aegypti, and analysed for glycoprotein and polypeptide constituents by isoelectric focusing and sodium dodecyl sulphate polyacrylamide gel electrophoresis. a major glycoprotein of molecular weight 110 000 carrying binding sites for concanavalin a and soybean agglutinin has been purified to homogeneity. although located on the cell surface, the 110 kdalton glycoprotein is not labelled by lactoperoxidase-catalysed ... | 1981 | 7213698 |
| [attractant properties of peptides for oviparous female aedes aegypti and culex pipiens molestus mosquitoes]. | 1980 | 7207386 | |
| brugia pahangi: development in aedes aegypti reared axenically on a defined synthetic diet. | 1981 | 7202630 | |
| [intake of microfilariae by the vector in the case of a low microfilareamia (author's transl)]. | the study of the intake of microfilariae of dipetalonema dessetae by aedes aegypti leads to two conclusions with respect to the microfilaraemia: --there is no concentration of microfilariae in the uptake of blood by aedes. --the microfilariae are nearly homogeneously distributed in the vertebrate host blood available to the vector for feeding. such a distribution of microfilariae in the cutaneous blood supply of the host gives the maximum chance for a mosquito to become infected when taking a bl ... | 1982 | 7200752 |