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[new epidemiological aspects of dengue].since the 1940s, the dengue epidemics occur more and more often in the pacific islands with an increased severity. for example, in new caledonia, outbreaks of dengue-like diseases have been reported since the end of the last century but the first epidemic due to an identified virus occurred in 1942-1943 and was caused by the den-1 type. the next, due to the den-2 type, was reported thirty years later, in 1972-1973. after that, three outbreaks burst between 1975 and 1990, caused successively by d ...19968924778
[control of aedes aegypti in martinique. contribution of entomology studies].the dengue prevention activities in martinique are based on: entomological surveillance (including the use of insecticide in all parts of the island). epidemiological (clinical, serological and virological) surveillance. health education (radio, tv, exhibitions, talks in primaries and secondaries schools, in associations,...). the main breeding sites of aedes aegypti are maintained by human practices: flowers vases, containers, used tyres, waste... the role of those human practices in the standi ...19968924777
[epidemiology of arbovirus diseases: use and value of physiologic age determination of female mosquito vectors].the physiological age of yellow fever aedes females in africa was studied during four years, from 1988 to 1992. we used a method, according to polovodova's method, which looks for the "yellow body" under natural light. those yellow bodies exist in the old females, the "parous" ones, and not in the young females, the "nulliparous" ones. we present some results to illustrate the interest of studying the physiological age of mosquitoes in the epidemiology of the arboviral diseases. the transmission ...19968924776
[anti-aedes aegypti campaign in french guiana].the history of vector control in french guiana started in 1947 when a small team was recruited for that purpose. in 1949, the first ddt treatments were implemented and aedes aegypti could not be found until 1960. between 1966 and 1972 an eradication campaign was carried out which resulted in the elimination of ae. aegypti from all the department except the city of cayenne. malathion and orthodibrom were used as adulticides and temephos (abate) was sprayed against the larvae. nevertheless, in 198 ...19968924775
[control of mosquitos in martinique. a collective public health action and improvement of the quality of life].aedes aegypti control program in martinique started en 1968. the aedes aegypti major breeding sites are associated with flowers vases, drums, used tyres, artificial containers and watering places. however, culex quinquefasciatus is the main nuisance mosquito in all the island. this species is unpleasant, especially in urban areas. over 200 culex quinquefasciatus bites per person per night have been observed in several localities of the island. culex quinquefasciatus breeding sites are mostly ass ...19968924774
[the prevention of dengue in guadeloupe].dengue is the only disease transmitted by mosquitoes in the guadeloupe islands of french antilles. two dengue outbreaks were reported in 1992 and 1994. aedes aegypti is recognized the only vector of dengue in guadeloupe, and the 200 l drums are the most important breeding-sites. vector control in guadeloupe includes community health education, larvicide and adulticide spraying, and the destruction of the breeding-sites. however, ae. aegypti control has limitations such as the lack of participati ...19968924773
[dengue and its vectors in brazil].history of dengue in brazil is covered from the first citations in the xixth century to the great outbreaks of the last ten years. den-1 and den-4 viruses have been isolated for the first time in 1982 during an epidemic in boa vista, roraima state. in 1986-1987, epidemics of dengue type 1 covered an extended area from rio de janeiro/sào paulo states to the north east states of brazil. during 1990-1991, dengue type 2 epidemics have been notified in the south east (rio de janeiro/são paulo) and in ...19968924771
[prediction and prevention of dengue epidemics].prediction and prevention of dengue epidemics are based on informations gathered about the mosquito vector species, the dengue types transmitted, the vertebrate hosts and their environment. although aedes aegypti is the most important vector, other aedes may also propagate the dengue viruses. the populations of vector mosquitoes are evaluated through several indices: breteau and/or positive house index, number of indoor resting aedes females, etc.... the four dengue types can replicate in verteb ...19968924770
[oviposition and dispersion of aedes aegypti in an urban environment].it is generally accepted that female aedes aegypti do not fly more than 50-100 m in their entire lifetime, yet the rapidity with which this species colonizes new areas, and the explosive nature of dengue and yellow fever epidemics appear to contradict this. using molecular methods, we have confirmed that the ae. aegypti females lay small numbers of eggs at many sites. the distribution of available sites implies that the female may fly a considerable distance to deposit her whole egg batch. we de ...19968924769
[aedes aegypti in french guiana. some aspects of history, general ecology and vertical transmission of the dengue virus].aedes aegypti is suspected to be present in the country since the late 18th century, and was responsible of urban yellow fever epidemics in the last century. this mosquito was identified for the first time in french guiana in 1902. more recently, in 1940, an eradication campaign started and aedes aegypti was eradicated between 1950 and 1963, date of the reinfestation. during the past 30 years, some dengue outbreaks occurred every 2 to 6 years, and the first dengue haemorrhagic fever epidemic spr ...19968924768
[the risk of urban yellow fever outbreaks in brazil by dengue vectors. aedes aegypti and aedes albopictus].urban yellow fever (yf) epidemics have disappeared from brazil since about 50 years, but a selvatic cycle still exist. in many states, cases are more or less numerous each year. ae. aegypti was eradicated in 1954, re-appeared temporarily in 1967, and then definitively in 1976-1977. ae. aegypti is a vector of yellow few (yf), but also of dengue, whose first cases were reported in 1982. today, dengue is endemic in many regions. a second flavivirus vector, aedes albopictus is present since about te ...19968924767
[ecology of aedes aegypti in africa and asia].aedes aegypti is originated from africa and expanded around the tropical world with a pantropical distribution in 1930. because of mosquito control, this extension decreased, but is still very important and threatening because of disease transmission. eggs of ae. aegypti can survive unfavorable conditions. larvae and pupae of ae. aegypti breed into natural or artificial containers, the last being the most important. artificial breeding-sites are mostly water storage containers and discarded cont ...19968924766
identification and characterization of differentially expressed cdnas of the vector mosquito, anopheles gambiae.the isolation and study of anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interactions. we have used the differential display technique to screen for mrnas specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females. we also screened for mrnas specifica ...19968917545
attraction and trapping of aedes aegypti (diptera: culicidae) with host odors in the laboratory.volatile constituents of mouse odor were eluted from an adsorbent after collection within a closed-air system, and offered to host-seeking yellowfever mosquitoes, aedes aegypti (l.), in the laboratory. gas chromatographic analysis showed that individual mouse odors varied in blend, both quantitatively and qualitatively. in a 2-choice bioassay, sticky traps baited with individual mouse odors caught more female mosquitoes than controls (69% caught on baited traps) in all cases. when 2 traps baited ...19968906926
occurrence of aedes (stegomyia) spp. mosquitoes (diptera: culicidae) in halmahela villages, indonesia.aedes (stegomyia) spp. mosquitoes breeding in containers in and around human houses were surveyed in halmahela villages, the north moluccas, indonesia. adults reared from larvae found in containers and those reared from eggs collected by ovitraps revealed the dominance of aedes scutellaris (walker) in residential areas. breeding of aedes aegypti (l.) and aedes albopictus (skuse) was confirmed in a few coastal villages with high human densities.19968906924
inter- and intraspecific variation in resistance to desiccation by adult aedes (stegomyia) spp. (diptera: culicidae) from indonesia.desiccation survival times of adult mosquitoes were studied for yellow fever mosquito, aedes aegypti (l.) (6 strains), asian tiger mosquito, ae. albopictus (skuse) (5 strains), and ae. paullusi stone & farmer (1 strain) colonized from south sulawesi, indonesia. at both 90 and 70% rh, ae. aegypti males and females outlived the other species. the forest species ae. paullusi was least resistant to desiccation. strains of ae. aegypti and ae. albopictus from urban areas were more resistant to desicca ...19968906905
involvement of peroxidase in chorion hardening in aedes aegypti.peroxidase activity is detectable in aedes aegypti ovaries, containing developing eggs, at 24 h following blood feeding, and peak peroxidase activity is reached at 36-48 h after the blood-meal. peroxidase is associated with the chorion layer in mature eggs and the majority of the enzyme is released from the chorion layer by treating the isolated chorion fraction with sds/urea. analysis of the sds/urea solubilized chorion proteins using sds-page with tropolone/h2o2 or dopa staining verified the p ...19968900599
purification and characterization of a major glutathione s-transferase from the mosquito anopheles dirus (species b).the major form of glutathione s-transferase (gst) activity from the mosquito anopheles dirus (species b), a vector of malaria in thailand has been purified 421-fold. it constituted approx. 20% of the total measured cdnb conjugating activity in the homogenate. this enzyme appeared as a single band of 25.0 +/- 0.26 kda on sds-page and was kinetically characterized with 10 substrates and 4 inhibitors. the enzyme is capable of catalysing dehydrochlorination of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl ...19968900597
responses of antennal olfactory receptors in the yellow fever mosquito aedes aegypti to human body odours.recent behavioural studies have demonstrated that human body odours which female aedes aegypti find attractive exert their effects as complex mixtures of synergistically acting components. we have attempted to clarify the sensory mechanisms underlying the perception of these complex host odours by studying the responses of sensory cells underneath the a3-type sensilla of the mosquito antenna to both a human skin wash extract and the extract's active chromatographic fractions. the reaction patter ...19968894302
sensory aspects of host location in mosquitoes.visual, thermal and olfactory stimuli all contribute to blood meal host location in mosquitoes but olfaction is probably the dominant sensory modality used for this purpose. much attention has been devoted to the l-lactic acid receptor because it is well characterized and because its sensitivity is a major determinant of host responsiveness in the anautogenous species aedes aegypti. studies employing statistical analysis of close to 500 single unit recordings and the scanning electron microscope ...19968894299
central olfactory pathways in mosquitoes and other insects.studies of cns processing of olfactory information have contributed significantly to understanding olfactory-guided behaviour in insects. evidence in moths suggests that each glomerulus in the antennal lobes has a unique property: receptor-axon projections and dendritic arborizations of uniglomerular output neurons can relate to anatomically and functionally distinct glomeruli. similar correlations are not typical of the concentrically organized locust antennal lobes. insights about odour proces ...19968894298
a search for components in human body odour that attract females of aedes aegypti.in a new type of wind tunnel, mosquitoes fly upwind towards host odour sources and towards human skin wash extracts obtained by rubbing the skin with a pad soaked in ethanol. we used this behavioural response as a bioassay to identify attractants in liquid chromatography fractions of such extracts. l-lactic acid is a major constituent of skin wash extracts and it is a necessary component for the extract's effectiveness. as a single stimulus, however, l-lactic acid is only slightly effective. thi ...19968894295
the mosquito ultraspiracle homologue, a partner of ecdysteroid receptor heterodimer: cloning and characterization of isoforms expressed during vitellogenesis.we report the cloning and characterization of two isoforms of the ultraspiracle homologue (aausp) from the mosquito, aedes aegypti. the 2.33-kb aauspa cdna has an open reading frame (orf) of 484 amino acids encoding a polypeptide of 54 kda, whereas the 2.14-kb aauspb orf of 459 amino acids encodes a 51.3 kda polypeptide. the aauspa and aauspb proteins differ only in the n-terminal portion of the variable a/b domain. the aausp dna-binding domain shares 92% and 97% identities with the respective d ...19968892313
heritability of infection-induced humoral antibacterial activity in the immune response of the mosquito aedes aegypti. 19968887345
adaptive larval thermotolerance and induced cross-tolerance to propoxur insecticide in mosquitoes anopheles stephensi and aedes aegypti.fourth-instar larvae of mosquitoes anopheles stephensi and aedes aegypti normally died within 90 min at 43 degrees c. pre-exposure to high but sublethal temperatures conferred adaptive thermotolerance, dependent on the temperature and the duration of pre-exposure. adaptive cross-tolerance to propoxur (a carbamate insecticide) was also induced in larvae by pre-exposing them to sublethal temperatures. pre-exposure to sublethal concentrations of propoxur was found to confer cross-thermotolerance to ...19968887340
effects of variations in a formulated protein meal on the fecundity and fertility of female mosquitoes.a formulated protein meal developed by kogan (1990) for adult female aedes aegypti mosquitoes was evaluated and modified to increase egg and pupal yield. a vigorous laboratory colony was maintained with the females fed exclusively on this dietary formula for about twenty-five generations over more than 2 years. extra modifications were made to produce a diet suitable for anopheles arabiensis and anopheles stephensi females to produce eggs. both formulations contain bovine albumin, haemoglobin an ...19968887337
discovery of aedes albopictus in guatemala.aedes albopictus was discovered for the first time in guatemala during june 1995. it was collected in 10 out of 15 localities sampled in and around the community of puerto barrios in the department of izabal. the location of the positive collections indicates a more rural distribution than aedes aegypti. sampling was conducted along the atlantic highway up to the capital city to determine the extent of its introduction into the country. larvae were not found more than 4 km outside of puerto barr ...19968887235
insecticide susceptibility of aedes aegypti and aedes albopictus in the lower rio grande valley of texas and mexico.in response to a potential dengue fever outbreak in south texas during 1995, the susceptibilities of aedes aegypti and aedes albopictus to commonly used mosquito adulticides were assessed. larvae collected from the lower rio grande valley of texas and mexico were reared to adults and tested against susceptible laboratory strains at texas a&m university. resistance ratios at both the lc50 and lc95 rates were all less than 10, indicating that adult populations of both species are still susceptible ...19968887230
mosquito vector control and biology in latin america--a sixth symposium.the sixth spanish language symposium presented by the american mosquito control association (amca) was held as part of the 62nd annual meeting in norfolk, va, in march 1996. the principal objective, as for the previous 5 symposia, was to promote the participation in the amca meeting by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 25 presentations that were given in spanish by participants from 6 countries in latin ...19968887226
early trypsin, a female-specific midgut protease in aedes aegypti: isolation, aminoterminal sequence determination, and cloning and sequencing of the gene.early trypsin is a female-specific protease present in the aedes aegypti midgut during the first hours after ingestion of a blood meal. it plays an essential role in the transcriptional activation of the late trypsin form, the major midgut endoprotease involved in the blood meal digestion. early trypsin is the most abundant midgut polypeptide isolated by benzamidine-sepharose affinity chromatography 3 h after feeding. the amino-terminal sequence of the early trypsin protein matches that of the 3 ...19968882654
setaria digitata microfilaraemia in mastomys coucha: an animal model for chemotherapeutic and immunobiological studies.intraperitoneal implantation of adult gravid females of the bovine filarial parasite, setaria digitata in mastomys coucha was found to induce microfilaraemia lasting for about 125 days. the microfilariae (mf) could be detected as early as 4 days post-implantation (p.i.) and peak levels of about 30 mf in 20 microliters of blood were observed by 21 days. a significant positive correlation was found between mf density and the body weight of recipients pre-implantation. the implanted adult worms wer ...19968873474
the efficacy of prrrikweg gel in the treatment of insect bites: a double-blind, placebo-controlled clinical trial.this study was conducted to examine the efficacy of prrikweg gel, a homeopathic after-bite gel, in relieving the effects of mosquito bites, in particular itching and erythema.19968861830
phenotypic segregation of aedes aegypti for immune antibacterial activity and resistance to filariae.we report the phenotypic selection of two lines of aedes aegypti, from a filariae susceptible parental stock (refm). this selection was based upon the level of inducible anti-gram negative escherichia coli activity within the haemolymph following e. coli infection. these lines, denoted 'high' and 'low', demonstrated significant differences in anti-e. coli responses throughout. however no difference was observed in the anti-gram positive micrococcus luteus response following e. coli challenge. f4 ...19968858873
acasp, a gene encoding a cathepsin d-like aspartic protease from the hookworm ancylostoma caninum.intestinal infection with the zoonotic hookworm ancylostoma caninum can provoke human eosinophilic enteritis. a cdna was isolated from a. caninum, using an oligonucleotide primer designed to hybridize to the region encoding the consensus, catalytic site residues d32tgssnlw of aspartic proteases. this novel cdna encoded an aspartic protease zymogen of 422 amino acids, exhibiting 47% identity to the lysosomal aspartic protease of aedes aegypti, 46% identity to the aspartic protease of schistosoma ...19968858139
cell targeting of a pore-forming toxin, cyta delta-endotoxin from bacillus thuringiensis subspecies israelensis, by conjugating cyta with anti-thy 1 monoclonal antibodies and insulin.the cytolytic protein toxin cyta was linked to two monoclonal antibodies (mab) directed against the mouse or the rat thy 1 antigen. the purified cyta-mab conjugates were not toxic to either target or nontarget cells. the conjugates did bind specifically to target cells since they agglutinated the target cells but not nontarget cells. when the conjugates were treated with dithiothreitol, the released cyta was toxic to all cells tested. these results suggested that the attachment of cyta to a mole ...19968853459
generation of expressed sequence tags and sequence-tagged sites as physical landmarks in the mosquito, aedes aegypti, genome.twenty-three clones were developed as expressed sequence tags (ests) and sequence-tagged sites (stss) that provide broad coverage of the mosquito, aedes aegypti, genome at an average spacing of about 7.2 cm. each of these clones had been mapped previously as a restriction fragment length polymorphism (rflp) marker. nineteen of these clones represent anonymous cdnas and 4 clones represent known genes. forty-seven percent of the anonymous cdnas showed significant deduced amino acid sequence simila ...19968851807
[yellow fever in the ribeirão preto region at the turn of the 19th century: its scientific importance and economic repercussions].yellow fever in the region of ribeirao preto at the turn of xix century: scientific importance and economic repercussion. this historical review describes the bad situation of public health in brazil during the xix century caused by multiple yellow fever outbreaks. the knowledge regarding to yellow fever at that time is also described. a short history is presented of the development of the region of ribeirao preto, located in the northeast of sao paulo state, brazil, emphasising the actuation of ...19968851220
intensive linkage mapping in a wasp (bracon hebetor) and a mosquito (aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic dna markers.the use of random amplified polymorphic dna from the polymerase chain reaction (rapd-pcr) allows efficient construction of saturated linkage maps. however, when analyzed by agarose gel electrophoresis, most rapd-pcr markers segregate as dominant alleles, reducing the amount of linkage information obtained. we describe the use of single strand conformation polymorphism (sscp) analysis of rapd markers to generate linkage maps in a haplodiploid parasitic wasp bracon (habrobracon) hebetor and a dipl ...08844159
enzymatic variability in natural populations of aedes aegypti (diptera:culicidae) from brazil.yellowfever mosquitoes, aedes aegypti (l), were collected from 3 towns located within 70 km of each other in the state of sao paulo to evaluate the protein variability of natural populations. electrophoretic analysis permitted the identification of 6 loci responsible for the production of leucine aminopeptidase, 3 for esterases and malic enzyme, 2 for malate dehydrogenase, isocitrate dehydrogenase and alpha-glycerophosphate dehydrogenase, and 1 for phosphoglucomutase. for the 3 populations, the ...19968840678
entomological studies in a dengue endemic area, jalore, rajasthan.entomological studies on prevalence of adult and immatures of aedes aegypti along with associated ecological factors have been conducted in a dengue endemic area of jalore, rajasthan from 1992 to 1993. studies in two areas; reportedly affected and unaffected revealed more adults and higher breeding indices in the affected area as compared to the unaffected one. mosquitoes harbouring dengue antigen were found only in the affected locality. presence of dengue antigen in field caught mosquitoes in ...19968840653
dengue 3 virus distribution in the mosquito aedes aegypti: an immunocytochemical study.the dissemination of dengue (den) 3 virus in parenterally infected female aedes aegypti mosquitoes was studied immunocytochemically. antigen was first detected in fat body cells near the thoracic site of virus inoculation. the intussuscepted foregut, salivary glands and nervous tissue were the first major tissues infected. nervous tissue appeared to be the primary site of amplification. muscles, tracheae, malphigian tubules and the posterior midgut did not become infected. the only part of the r ...19968834747
yellow fever: a decade of reemergence.since the 1980s, yellow fever has reemerged across africa and in south america. the total of 18 735 yellow fever cases and 4522 deaths reported from 1987 to 1991 represents the greatest amount of yellow fever activity reported to the world health organization (who) for any 5-year period since 1948. there is an excellent vaccine against yellow fever. at present, a high proportion of travelers to at-risk areas are reported to be immunized, reflecting widespread knowledge about the international he ...19968827969
stability of equine infectious anemia virus in aedes aegypti (diptera: culicidae), stomoxys calcitrans (diptera:muscidae), and tabanus fuscicostatus (diptera:tabanidae) stored at -70 degrees c.equine infectious anemia virus (eiav) was injected intrathoracically into aedes aegypti, stomoxys calcitrans, and tabanus fuscicostatus, and fed to ae. aegypti in suspensions of either artificial blood of eagle's minimum essential medium. insects were stored at -70 degrees c for up to 9 months before testing for the presence of eiav. the viral tissue culture titers detected from stored insects were similar to those from insects tested at time 0.19968827617
response of anopheles farauti to permethrin-treated net and cloth fabrics in the laboratory.the median effective dose of permethrin for knockdown and mortality of anopheles farauti s. s. exposed to 2 net and 2 cloth fabrics was estimated. there were no significant differences in the knockdown or mortality of an. farauti or aedes aegypti following 15-180-sec exposure to permethrin-treated bed nets and polyester/cotton fabric. despite the presence of permethrin (0.007-0.068 mg/cm2) in bed net material, 18.2-50% of an. farauti adults successfully obtained a blood meal through the nets, an ...19968827613
laboratory and field plot bioassay of bacillus sphaericus against arkansas mosquito species.bacillus sphaericus was evaluated in laboratory bioassays against larvae of 7 arkansas mosquito species and in rice field plots against a single mosquito species. laboratory-tested species included aedes aegypti, ae. albopictus, ae. vexans, anopheles punctipennis, culex quinquefasciatus, cx. restuans, and psorophora columbiae. field plot bioassays were evaluated against ps. columbiae only. the laboratory formulation tested demonstrated activity against all 7 species. mortalities were observed at ...19968827596
community based dengue control in columbia: people's knowledge and practice and the potential contribution of the biological larvicide bti (bacillus thuringiensis israelensis).a study on dengue control was undertaken in a poor urban area in cúcuta, colombia. the first objective was to describe people's knowledge, attitudes and practices regarding dengue fever, the transmission of the disease and possible preventive measures. the second objective was to analyse the infestation of the community with aedes aegypti larvae, and the third objective to test the efficacy of bti (bacillus thuringiensis israelensis) with respect to the level and duration of reduction of ae. aeg ...19958826104
evaluation of entomopathogenic bacteria against aedes polynesiensis, the vector of lymphatic filariasis in french polynesia.thirteen strains among 3 species of entomopathogenic bacteria were tested against 3 medically important mosquito species in french polynesia. two strains of bacillus thuringiensis were highly toxic to aedes polynesiensis, aedes aegypti, and culex quinquefasciatus. six of 7 strains of bacillus sphaericus tested were highly toxic to cx. quinquefasciatus but not to the aedes spp. clostridium bifermentans serovar. malaysia was more toxic to ae. polynesiensis than to the other 2 species. entomopathog ...19958825516
a new serovar of bacillus thuringiensis possessing 28a28c flagellar antigenic structure: bacillus thuringiensis serovar jegathesan, selectively toxic against mosquito larvae.a novel bacillus thuringiensis strain highly toxic to mosquitoes was isolated from soil samples in malaysia. this strain was shown to display a new subfraction of the h-28 flagellar antigen determining a new serovar h28a28c, which was designated serovar jegathesan. bioassays indicated that culex quinquefasciatus larvae are the most susceptible to this new isolate, whereas toxicity to anopheles maculatus and aedes aegypti larvae was 10 times lower. the potency of this new serotype is also compara ...19958825511
genetic differentiation of aedes aegypti, the vector of dengue virus in french polynesia.in recent years the incidence of dengue fever epidemics has increased and transmission has tended to be established over a geographically expanding area, including french polynesia. an increase in air transportation contributes to the diffusion of the dengue virus from southeast asia, a region considered to be a hyperendemic dengue zone, to the pacific region. presently, little is known about the role of the vector (aedes aegypti) in the diffusion of the dengue fever virus. a study on the geneti ...19958825508
country-wide prevalence of ascogregarina culicis (apicomplexa: lecudinidae), a protozoan parasite of aedes aegypti in trinidad, west indies.the prevalence of the gregarine parasite ascogregarina culicis was determined for aedes aegypti populations in trinidad, west indies. over 5 months, a total of 5,651 ae. aegypti larvae were collected and identified from 419 sites throughout trinidad as part of a national dengue surveillance program. ascogregarina culicis infections were detected in 13.4% of 1,986 ae. aegypti larvae dissected. in 7 of 8 counties or major subdivisions where this parasite was detected in trinidad, the prevalence pe ...19958825500
survey of container-inhabiting mosquitoes in clemson, south carolina, with emphasis on aedes albopictus.a total of 530 oviposition trap samples were collected within a 10-km radius of clemson university between march 30 and october 19, 1993. from 19,664 larvae reared from collected eggs, 7 species were identified: aedes albopictus (89%), ae. triseriatus (6.5%), culex restuans (2.7%), cx. territans (0.6%), cx. pipiens complex (0.7%), toxorhynchites rutilus septentrionalis (0.2%), and orthopodomyia signifera (0.1%). this is the first record of ae. albopictus in clemson. aedes aegypti was not found. ...19958825496
larvicidal constituents of melantheria albinervia.bioactivity-guided fractionation has led to the isolation of two larvicidal diterpenes, active against the yellow fever-transmitting mosquito aedes aegypti, from a dichloromethane root extract of melantheria albinervia (asteraceae), a plant from zimbabwe. these diterpenes were identified as ent-kaur-16-en-19-oic acid and 9(11),16-kauradien-19-oic acid. the diterpenes were also weakly active against bacillus subtilis.19958824954
cloning and characterization of cdnas encoding the antibacterial peptide, defensin a, from the mosquito, aedes aegypti.insect defensins are cationic, inducible antibacterial peptides. four full-length cdnas encoding defensin a from the mosquito aedes aegypti were cloned using polymerase chain reaction (pcr) and sequenced. all four cdnas are 473 base pairs long, bearing an open reading frame of 98 amino acids with a few substitutions in the signal peptide domain. the deduced amino acid sequence of aedes aegypti defensin (aadef) contains a signal peptide sequence of 18 amino acids followed by a 40-amino acid putat ...19968814787
evaluation of bacillus thuringiensis and bacillus sphaericus strains from chinese soils toxic to mosquito larvaeduring our research to isolate and screen microbial agents, eight bacillus thuringiensis isolates and five bacillus sphaericus isolates were shown to have high toxicity to mosquito larvae. comparing the lc50 values, four b. sphaericus isolates with lc50 values ranging from 0.50 to 1.47 ng/ml were about two to six times more toxic than strain 1593 (lc50 3.00 ng/ml) against larvae of culex quinquefasciatus. four b. thuringiensis isolates (lc50 values ranging from 3.80 to 7.54 ng/ml) and four b. sp ...19968812574
effect of inactivation by sunlight on the larvicidal activities of mosquitocidal bacillus thuringiensis h-14 isolates from nigerian soils.bacillus thuringiensis h-14 is a well recognized bioinsecticide against mosquitoes. this study investigates the inactivation effect of sunlight on the larvicidal activities of four nigerian isolates of b. thuringiensis h-14, code-named obg1, obg8, gsc3 and gna13 as compared with a standard mosquito larvicide, b. thuringiensis var. israelensis (bti). a 0.125 mg/1 suspension of spore-delta-endotoxin powder of each isolate was exposed to direct sunlight for 7 h on a concrete roof (ca. 13 m high). a ...19968810143
bioefficacy of mosquito mat, coil and dispenser formulations containing allethrin group of synthetic pyrethroids against mosquito vectors.the bioefficacy of mats, coils and dispensers containing allethrin group of synthetic pyrethroids was studied against laboratory strains of culex quinquefasciatus, aedes aegypti and anopheles stephensi. except esbiothrin in the mat formulation which was 100 and 178 times more effective against cx. quinquefasciatus than against ae. aegypti and an. stephensi with kt50 of 0.005, 0.5 and 0.89 min. respectively, all the three allethrins in mat or coil formulations were equally effective against the t ...19968810142
effect of cetirizine on the inflammatory cells in mosquito bites.mosquito bites frequently cause wealing and delayed papules which appear within a few hours after the bites and may persist for several days. cetirizine is an effective drug against mosquito bites by decreasing wealing and also the size and pruritus of the delayed bite papules.19968809428
cloning and expression analysis of aedes aegypti opsin: adaptation of an in situ hybridization protocol for mosquitoes.opsin is a g protein coupled photoreceptor that activates a signal transduction cascade in the ommatidia. its primary and secondary structure is conserved both in insects and vertebrates as exemplified by the drosophila opsins. through serendiplious cloning of a pcr fragment, we have identified an opsin cdna. the latter was used to clone full length cdnas from a mosquito head library. the main purpose of cloning was to have a positive control probe to establish an in situ hybridization protocol ...19968799735
molecular characterization of the mosquito vitellogenin receptor reveals unexpected high homology to the drosophila yolk protein receptor.the mosquito (aedes aegypti) vitellogenin receptor (aavgr) is a large membrane-bound protein (214 kda when linearized) that mediates internalization of vitellogenin, the major yolk-protein precursor, by oocytes during egg development. we have cloned and sequenced two cdna fragments encompassing the entire coding region of aavgr mrna, to our knowledge the first insect vgr sequence to be reported. the 7.3-kb aavgr mrna is present only in female germ-line cells and is abundant in previtellogenic oo ...19968799131
laboratory evaluation of toxic baits for mosquito (aedes aegypti) larvae.toxic bait discs were prepared by incorporating the larvicide abate (temephos) technical, with yeast and dog biscuit (1:1), the latter forming the attractant source. these were assessed in 1, 10 and 25 litre aquaria and 100 litre drums against aedes aegypti larvae. floating as well as sinking bait discs were found equally effective in controlling free moving larvae. larvae confined in artificially suspended cages were not affected. the baits were found effective for 5 to 6 weeks. toxic disc bait ...19968781040
[human diffusion of arthropod disease vectors throughout the world].the present distribution of animals and plants throughout the world is the result of an evolutionary process involving tectonic, climatic and biotic factors. humans, since their appearance, have contributed to the spreading of many species including disease vectors and pests. when humans left their native african home, they brought with them ectoparasites such as lice and acarids. during the neolithic era, humans were leading domesticated animals which carried their own parasites into new areas. ...19958777543
monoclonal antibodies against manduca sexta hemocytes bind aedes aegypti hemocytes: characterization of six monoclonal antibodies that bind hemocytes from both species.the mechanisms by which hemocytes mediate a mosquito's defense response to parasites or pathogens are not well understood. this is due in part to difficulty in collecting intact mosquito hemocytes for experiments and to a lack of reagents, such as antibodies. our objectives were to collect adult aedes aegypti hemocytes under conditions suitable for immunofluorescence microscopy, and to test whether monoclonal antibodies, generated against larval manduca sexta hemocytes, bind adult ae. aegypti he ...19958773196
epidemic dengue 2 in the city of djibouti 1991-1992.from october 1991 to february 1992, an outbreak of acute fever (in which thick blood films were negative for malaria) spread rapidly in the city of djibouti, djibouti republic, affecting all age groups and both nationals and foreigners. the estimated number of cases was 12,000. the clinical features were consistent with a non-haemorrhagic dengue-like illness. serum samples from 91 patients were analysed serologically for flavivirus infection (dengue 1-4, west nile, yellow fever, zika, banzi, and ...19968758061
activity of novel juvenoids on arthropods of veterinary importance.different juvenile hormone mimics were compared regarding their efficacy against larval and pupal stages of the diptera aedes aegypti, musca domestica, and lucilia cuprina as well as the cat flea ctenocephalides felis. structure-activity relationship of a series of new diphenylethers was analyzed and compared to juvenoids like methoprene, hydroprene, and fenoxycarb. selected compounds were taken for a competition binding assay and their effect on jh-degradation by esterases was examined. the res ...19968756312
a placebo controlled clinical trial investigating the efficacy of a homeopathic after-bite gel in reducing mosquito bite induced erythema.a randomised, placebo controlled clinical trial was conducted to examine the efficacy of a homeopathic after-bite gel in the symptomatic relief of mosquito bites. sixty eight healthy volunteers were bitten under laboratory conditions by aedes aegypti mosquitoes at three spots, on the ventral aspect of the forearm. one bite was treated with the homeopathic after-bite gel, another bite with a placebo gel which was identical in appearance and smell to the homeopathic after-bite gel, and the third b ...19958751030
surveillance of the mosquito aedes aegypti and its biocontrol with the copepod mesocyclops aspericornis in australian wells and gold mines.a survey of the dengue vector mosquito aedes aegypti was undertaken using funnel traps to detect immature stages (larvae and pupae) in flooded disused mine shafts and wells in charters towers, queensland, northern australia. the town has a history of dengue fever since 1885 when goldminers were the first recorded victims. during the latest dengue epidemic in 1993, 2% of the population had laboratory-confirmed dengue virus type 2, despite source reduction of ae.aegypti breeding-sites at ground le ...19968744708
community involvement in the control of aedes aegypti.in the past 15 years, there has been a dramatic resurgence of dengue and dengue hemorrhagic fever worldwide, with increased frequency of epidemics and geographic expansion of both the mosquito vectors and the viruses. the reasons for this resurgence are not well understood, but include uncontrolled and unplanned urbanization, increased movement of people and viruses by airplane and lack of effective control of aedes aegypti, the principal mosquito vector of dengue viruses. the recommended method ...19968740894
quiescent ovarioles in the mosquito aedes aegypti (l.) (diptera: culicidae). 19968729634
ovulation and post-ovulation changes in the terminal region of the ovarioles of the mosquito aedes aegypti (l.) (diptera: culicidae).detachment of the follicular epithelium from the chorion occurs prior to the passage of the mature egg into the calyx lumen, resulting in a mature oocyte lying free in an epithelial sac. the mature oocytes rupture the epithelial sac, including the tunica propria at the posterior pole, then break through the basal (or granular basal) bodies to enter the calyx lumen. just after ovulation, the formerly terminal parts of the ovariole remain attached to the posterior end of the egg sac near its openi ...19968729630
lipid transfer from insect fat body to lipophorin: comparison between a mosquito triacylglycerol-rich lipophorin and a sphinx moth diacylglycerol-rich lipophorin.two insect lipoproteins, triacylglycerol-rich aedes aegypti lipophorin and diacylglycerol-rich manduca sexta lipophorin, were compared in their ability to load neutral lipid from fat body. when fat body of m. sexta was incubated in vitro with [3h]oleic acid, all radiolabeled fatty acids were esterified, predominantly to triacylglycerol. in a. aegypti fat body, however, half of the label remained as free fatty acids. when a. aegypti fat body was radiolabeled with [3h]glycerol, most of the radiola ...19968725165
evaluation of the laboratory rabbit model for screening topical mosquito repellents.the laboratory rabbit was evaluated as a model for screening topical mosquito repellents, using data obtained in tests of deet (n,n-diethyl-3-methylbenzamide) against aedes aegypti on humans and rabbits. host-specific differences in the action of the test material were quantified by multiple regression analysis. the test material was less effective but more persistent in tests on rabbits, and responses of the mosquito test population were more variable.19968723273
evaluation of controlled-release mosquito repellent formulations.eight polymer and 9 microcapsule formulations of deet were tested on laboratory rabbits against aedes aegypti and anopheles albimanus. several formulations were significantly more effective than simple (unformulated) deet at the same strength for periods up to 24 h. best results were obtained with a polymer formulation containing a high molecular weight fatty acid and 3 microcapsule formulations containing lanolin, gum arabic, gelatin, tannic acid, stearic acid, polypropylene glycol, water, and ...19968723256
yellowfever mosquito (diptera:culicidae) introduced into landi kotal, pakistan, by tire importation.although the recent distribution of yellowfever mosquito, aedes aegypti (l.), in pakistan has been restricted to the port city of karachi, adult and immature mosquitoes breeding in imported tires in warehouses at landi kotal (north-west frontier province) were identified as ae. aegypti. the patterns of tire trade and the current disjunct distribution of ae. aegypti indicated that the introduction into landi kotal may have been either from karachi or india. thermal fog application of pirimiphos-m ...19968699469
characterization of the clinical and anatomical pathological changes associated with hepatozoon mocassini infections in unnatural reptilian hosts.laboratory-reared aedes aegypti mosquitoes were employed in the successful transmission of hepatozoon mocassini from a cotton-mouth moccasin (agkistrodon piscivorus leucostoma) to 3 lizard species (sceloporus undulatus, eumeces obsoletus and sceloporus poinsetti). marked to severe lethargy and anorexia developed in the s. undulatus, e. obsoletus and s. poinsetti at 15, 38, and 96 days postinfection (pi), respectively. all 3 lizards developed a leukocytosis and had increased plasma aspartate amin ...19968690537
aedes aegypti: induced antibacterial proteins reduce the establishment and development of brugia malayi.the effect of host immune activation on the development of brugia malayi in one susceptible and four refractory strains of aedes aegypti and in armigeres subalbatus was assessed. a. aegypti that were immune activated by the injection of saline or bacteria 24 hr before feeding on a b. malayi-infected gerbil had significantly reduced prevalences and mean intensities of infection from those of naive controls when exposed to bloodmeals with low (105 mf/20 microliters) and medium (160 mf/20 microlite ...19968682188
mosquito dopa decarboxylase cdna characterization and blood-meal-induced ovarian expression.dopa decarboxylase (ddc) functions in insect catecholamine biochemistry to produce materials essential for cross-linking reactions that result in tanning and/or melanization, include tanning of the mosquito egg chorion and encapsulation of parasites. we have cloned ddc from the mosquito, aedes aegypti, and studied its expression in response to blood-feeding, which initiates events necessary for egg maturation in mosquitoes. the ae. aegypti ddc cdna was isolated via heterologous screening using a ...19968673262
impact of rapid urbanization on mosquitoes and their disease transmission potential in accra and tema, ghana.the total of 75 mosquito species recorded in accra have declined to 28 species. contributing factors to this decline and the reduction in prevalence of malaria and bancroftian filariasis in accra presently include extensive water pollution and a fairly high daily mosquito mortality due to several factors including loss of natural adult resting places, use of mosquito repellents and the probable increase of anopheles arabiensis population. presently low yellow fever incidence is due inter alia to ...19958669399
[the residual effect of temephos on aedes aegypti larvae].one of the most used strategies for controlling aedes aegypti is the use of the larvicide temephós (abate). this larvicide has a prolonged residual effect which allows planning the focal treatments. this study aims to verify the duration of the larvicide residual effect stimulating a field situation. plastic containers of one and five liters were treated with temephós and the residual effect was evaluated every 30 day after the treatment. different residual effect was observed in the containers ...20138668838
experimental evidence against replication or dissemination of hepatitis c virus in mosquitoes (diptera:culicidae) using detection by reverse transcriptase polymerase chain reaction.in 3 laboratory experiments, mosquitoes were fed hepatitis c virus (hcv)-rna positive blood by using membrane feeders, separated into head, thorax, and abdomen, and tested by a reverse transcriptase polymerase chain reaction for hcv-rna. hcv did not replicate or disseminate in mosquitoes that had ingested blood from patients that were hcv-viremic positive. when yellow fever mosquitoes, aedes aegypti (l.), were held for 1, 3, 7, 14, and 21 d after feeding, hcv-rna was detected in the abdomens of ...19968667387
cloning and expression of the first anaerobic toxin gene from clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to bacillus thuringiensis delta-endotoxins.a gene (cbm71) encoding a 71,128-da mosquitocidal protein (cbm71) was obtained by screening a size-fractionated xbai digest of total genomic dna from clostridium bifermentans subsp. malaysia ch18 with two gene-specific oligonucleotide probes. the sequence of the cbm71 protein, as deduced from the sequence of cbm71, corresponds to that of the 66-kda protein previously described as one of the mosquitocidal components of c. bifermentans subsp. malaysia. cbm71 shows limited similarities with bacillu ...19968655486
functional analysis of a mosquito gamma-aminobutyric acid receptor gene promoter.a single point mutation in the insect gamma-aminobutyric acid receptor (gabar)-encoding gene (rdl) confers high levels of resistance to cyclodienes in drosophila and other insects. we were interested in studying the promoter of this gene for two reasons. firstly, to define the elements underlying rdl expression. secondly, to identify the minimum set of regulatory elements necessary for construction of a functional rdl minigene. such an insecticide-resistance-associated minigene should form a str ...19968654932
subunit cleavage of mosquito pro-vitellogenin by a subtilisin-like convertase.the eukaryotic convertase family plays an important role in posttranslational proteolytic processing and activation of many pro- and polypeptides that have at their cleavage sites the paired basic motif, rx(k/r)r. recent studies have revealed that the cleavage site of insect pro-vitellogenins (pro-vg) also contains this motif. to identify and characterize the insect pro-vg processing enzyme, vg convertase (vc), its cdna was cloned from a vitellogenic female fat body cdna library of the mosquito, ...08650241
cloning and sequencing of trehalose synthase gene from pimelobacter sp. r48.the gene encoding trehalose synthase (catalyzing the conversion of maltose into alpha, alpha-trehalose by intramolecular transglucosylation) was cloned from pimelobacter sp. r48. sequence analysis revealed a 1719-bp synthase gene and a 573-residue amino-acid sequence. the 220 n-terminal residues were homologous to those of maltases from saccharomyces carlsbergensis and aedes aegypti.19968645698
critical examination of aedes aegypti indices: correlations with abundance.the following immature stage indices for aedes (stegomyia) aegypti surveillance were evaluated in four north queensland, australia towns with respect to their relationship to immature and adult female densities: breteau, house, container, larval density, stegomyia (and modifications thereof), and a newly created adult productivity index. spearman's correlations of indices that considered larval or immature (larvae and pupae) numbers had a better relationship with immature abundance but this was ...19968644913
antibody-mediated inhibition of aedes aegypti midgut trypsins blocks sporogonic development of plasmodium gallinaceum.the peritrophic matrix (pm) that forms around a blood meal is a potential barrier of plasmodium development in mosquitoes. previously, we have shown that to traverse the pm, plasmodium ookinetes secrete a prochitinase and that an inhibitor of chitinase blocks further parasite development. here we report that it is the mosquito trypsin that activates the plasmodium prochitinase. trypsin was identified as the chitinase-activating enzyme by two criteria: (i) trypsin activity and activating activity ...19968641775
cloning and developmental expression of the ecdysone receptor gene from the spruce budworm, choristoneura fumiferana.degenerate oligonucleotides were designed on the basis of conserved amino acid sequences in the dna and ligand-binding regions of the members of the steroid hormone receptor superfamily. using these oligonucleotides in rna-pcr, a cdna fragment was isolated from the spruce budworm, choristoneura fumiferana. comparison of the deduced amino acid sequence of this cdna fragment with the members of the steroid hormone receptor superfamily suggested that this pcr fragment is a region of the ecdysone re ...19958641050
aedes aegypti midgut early trypsin is post-transcriptionally regulated by blood feeding.early trypsin is a female-specific protease present in the aedes aegypti midgut during the first hours after ingestion of a blood meal. early trypsin gene expression was studied by northern blot analysis. the early trypsin mrna, absent in larvae, pupae and newly emerged females, reaches detectable levels at 24 h post-emergence and attains a maximum level at an adult age of 4-7 days. after the first week there is a decrease in the steady-state level of the transcript, but it remains readily detec ...19968630532
genetically engineered resistance to dengue-2 virus transmission in mosquitoes.the control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. to reduce the ability of mosquitoes to transmit dengue viruses, a recombinant sindbis virus was used to transduce female aedes aegypti with a 567-base antisense rna targeted to the premembrane coding region of dengue type 2 (den-2) virus. the transduced mosquitoes were unable to support replication of den-2 virus in t ...19968629025
population genetics with rapd-pcr markers: the breeding structure of aedes aegypti in puerto rico.rapd-pcr polymorphisms at 57 presumptive loci were used to examine the breeding structure of the mosquito aedes aegypti in puerto rico. mosquitoes were sampled from 16 locations in six cities and samples were located in a nested spatial design to examine local patterns of gene flow. allele frequencies were estimated assuming (1) that genomic regions amplified by rapd-pcr segregate as dominant alleles, (2) that genotypes at rapd loci are in hardy-weinberg proportions, (3) identity in state (iis) ...19968626220
possible contributing factors to the paucity of yellow fever epidemics in the ashanti region of ghana, west africa.yellow fever virus vectors identified in the ashanti region of ghana included aedes aegypti, aedes africanus, aedes luteocephalus and aedes vittatus. other mosquito species, unrelated to yellow fever transmission, identified in this study included culex tigripes, culex thalassius, culex decens, culex tarsalis, anopheles gambiae, anopheles stephansi and toxorynchites brevipalpis. factors generally known to influence yellow fever transmission were also studied in the ashanti region. these included ...19968625858
mosquitoes, models, and dengue.in the last 10 years dengue has spread markedly through latin america and the caribbean (dominican republic, jamaica, barbados, mexico, guatemala, el salvador, honduras, nicaragua, costa rica, panama, ecuador, colombia, venezuela, and brazil). the mosquito aedes aegypti has taken advantage of increased urbanization and crowding to transmit the dengue virus. the mosquito infests tires, cans, and water jars near dwellings. the female mosquito practices multiple, interrupted feeding. thus, mosq ...19968622446
stable expression of insect gaba receptors in insect cell lines. promoters for efficient expression of drosophila and mosquito rdl gaba receptors in stably transformed mosquito cell lines.we are interested in establishing stably transformed insect cell lines efficiently expressing the insect gamma-aminobutyric acid (gaba) receptor subunit gene resistance to dieldrin or rdl. in order to facilitate this we utilized a system based on stable transformation of aedes albopictus mosquito cell lines using the dihydrofolate reductase (dhfr) gene as a selected marker. here we report the production of stable mosquito cell lines carrying high copy numbers of rdl genes from both drosophila an ...19968601436
[vectors of dengue in mexico: a critical review)].the mosquito aedes (stegomyia) aegypti (linneaus, 1762) is the vector of dengue viruses in america. recently, another related species, aedes albopictus (skuse, 1894) was first introduced to america in 1986 in texas, usa and later on in sao paulo, brazil. since then, this mosquito has rapidly colonized new areas along the northern border states of mexico. the importance of this species as a vector of dengue virus is not clear. this work presents a revision of some characteristics of the two speci ...19958599149
[the role of the aedes aegypti vector in the epidemiology of dengue in mexico].the role of aedes aegypti (lineo) in the epidemiology of dengue fever in mexico is herein discussed based on the vectorial capacity model. comments on the advantages and disadvantages of each model component at the time of field determinations are also presented. emphasis is made on the impact of sampling and method bias on the results of vectorial capacity studies. the paper also addresses the need to increase vector biology knowledge as an input for epidemiological work to explain and predict ...19958599147
[dengue in mexico: a priority problem of public health].dengue fever is a viral disease transmitted by aedes aegypti. it has been recently incorporated to the agenda of priorities in many countries in the americas. the emergence of dengue hemorrhagic fever cases is becoming a public health threat and requires the implementation of intensive surveillance and control strategies. the epidemiological panorama of dengue in mexico provides the opportunity to analyze a regional health problem and the risk factors involved in the genesis of hemorrhagic cases ...19958599144
[yellow fever in mexico. the eradication of aëdes aegypti. 1966]. 19958599142
jungle yellow fever: clinical and laboratorial studies emphasizing viremia on a human case.the authors report the clinical, laboratorial and epidemiological aspects of a human case of jungle yellow fever. the patient suffered from fever, chills, sweating, headaches, backaches, myalgia, epigastric pains, nausea, vomiting, diarrhea and prostration. he was unvaccinated and had been working in areas where cases of jungle yellow fever had been confirmed. investigations concerning the yellow fever virus were performed. blood samples were collected on several days in the course of the illnes ...19958599063
mosquitocidal activity of the cryic delta-endotoxin from bacillus thuringiensis subsp. aizawai.the cloned 135-kda cryic delta-endotoxin from bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against spodoptera litoralis and spodoptera frugiperda larvae and in vitro against the s. frugiperda sf9 cell line. here, we report that the cryic delta-endotoxin cloned from b. thuringienesis subsp. aizawai hd-229 and expressed in an acrystalliferous b. thuringiensis strain is also toxic to aedes aegypti, anophles gambiae, and culex quinquefasciatus mosquito larv ...19968593070
toward a physical map of aedes aegypti.labelled recombinant cosmids were used as in situ hybridization probes to aedes aegypti metaphase chromosomes. the cosmid probes yielded paired signals, one on each arm of sister chromatids, and they were ordered along the three chromosomes. in total, thirty-seven different probes were mapped to the three chromosomes of ae. aegypti (2n = 6): twenty-eight to chromosome 1, six to chromosome 2, and six to chromosome 3. these results represent an initial stage in the generation of a physical map of ...19958589842
immunohistological localization of regulatory peptides in the midgut of the female mosquito aedes aegypti.the midgut of the female mosquito aedes aegypti was studied immunohistologically with antisera to various regulatory peptides. endocrine cells immunoreactive with antisera to perisulfakinin, rfamide, bovine pancreatic polypeptide, urotensin 1, locustatachykinin 2 and allatostatins a1 and b2 were found in the midgut. perisulfakinin, rfamide and bovine pancreatic polypeptide all react with the same, about 500 endocrine cells, which were evenly distributed throughout the posterior midgut, with the ...19958574883
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