Publications

antibodies to proteus in rheumatoid arthritis.		1991	1913013
rheumatoid arthritis as an infectious disease.		1991	1912876
elevated levels of stress proteins associated with bacterial symbiosis in amoeba proteus and soybean root nodule cells.	obligatory bacterial endosymbionts of amoeba proteus and symbiotic bradyrhizobium japonicum bacteroids in soybean-root nodules contained large amounts of 67-kda and 65-kda proteins, respectively, antigenically related to groel of e. coli and the 58-kda heat-shock protein of tetrahymena. monoclonal antibodies against the 67-kda protein recognized groel analogs from several different organisms. the quantity of the stress protein in symbiotic b. japonicum bacteroids was augmented seven times that i ...	1991	1912387
[in vitro antibacterial activity of a new oral cephalosporin, ceftibuten. results of a multicenter study].	minimal inhibitory concentration (mic) of ceftibuten (cbt) were evaluated by agar dilution for 1,416 bacterial strains isolated in 5 hospitals. for enterobacteriaceae, mic 50 and 90% were respectively (micrograms/ml): (i) naturally non beta-lactamase producing species: e. coli 0.12-0.5, shigella 0.06-0.12 and salmonella 0.03-0.12, p. mirabilis 0.16-0.03. (ii) chromosomal penicillinase producing species: k. pneumoniae 0.03-0.5 and k. oxytoca 0.03-0.06. (iii) chromosomal cephalosporin producing sp ...	1991	1909017
activity of some antiseptics against urinary tract pathogens growing as biofilms on silicone surfaces.	the activity of chlorhexidine, mandelic acid and a mandelic/lactic mixture were tested against biofilms of four species of gram-negative nosocomial organisms that commonly infect the catheterized urinary tract. cells growing on silicone discs were exposed to concentrations of agents used in bladder instillation. citrobacter diversus biofilms proved to be sensitive to all three agents. pseudomonas aeruginosa, proteus mirabilis and klebsiella pneumoniae all survived well in chlorhexidine but their ...	1991	1908381
chronic urinary catheter blockage.	catheter blockage is a common complication of long-term urinary catheterisation which causes distress to the patient and presents management problems for both nursing and medical practitioners. jennifer kohler-ockmore's study aimed to investigate some of the factors which may cause catheter blockage and how they may be overcome. fifty-four patients, resident in the community, were monitored over a period of six months. the findings revealed that patients were divided into two groups, those with ...	1991	1907481
hydrogen peroxide as an alternative hatching egg disinfectant.	the present study examined the effectiveness of h2o2 at different concentrations to disinfect broiler hatching eggshell surfaces and to maintain hatching potential. under pure culture conditions, .50% h2o2 yielded over a 6 log kill in 30 s of three potential eggshell bacterial contaminants. under higher h2o2 demands, such as occurs on eggshell surfaces, h2o2 concentrations of 5% (vol/vol) were required to disinfect the shell surfaces (approximately 5 log reduction). hatchability of fertile eggs ...	1991	1906612
in vitro and in vivo evaluation of ro 09-1428, a new parenteral cephalosporin with high antipseudomonal activity.	ro 09-1428, a new parenteral cephalosporin with a catechol moiety attached at position 7 of the cephalosporin ring, showed high in vitro activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, and streptococcus pyogenes, with mics for 90% of strains tested (mic90s) of less than or equal to 0.39 micrograms/ml. morganella morganii, providencia rettgeri, citrobacter freundii, haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae were inhi ...	1991	1906261
[combination antibacterial effects between aztreonam and eight other antibiotics].	in vitro interactions between aztreonam (azt) and 8 other antibiotics were studied using the agar dilution checkerboard technique against 88 clinical isolates of escherichia coli, proteus vulgaris, serratia marcescens and pseudomonas aeruginosa. combinations of azt with 8 other antibiotics were generally additive or indifferent. synergism was occasionally seen against s. marcescens or p. aeruginosa with azt plus isepamicin (isp). antagonism was observed only between azt and latamoxef against p. ...	1991	1904106
further observations on enhancement of the infectivity of fusobacterium necrophorum by other bacteria.	it had already been shown with a single virulent strain (a42) of fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. the present study extended the known range of bacteria with strong infectivity-enhancing properties to include bacillus cereus, klebsiella oxytoca and staphylococcus aureus; an ...	1991	1902184
rhythmic bacterial susceptibility to antibiotics at a large hospital.	the in vitro susceptibility response of staphylococcus aureus, klebsiella pneumoniae, escherichia coli, proteus mirabilis and pseudomonas aeruginosa to a set of antibiotics was investigated in a survey comprising 19,380 positive cultures over a period of 5 years in a large hospital environment. four out of the five species (p. aeruginosa being the exception) presented a species-specific, drug-independent, rhythmic variation of their level of susceptibility to several antibiotics over the time of ...	1991	1899881
biofilms and urethral catheter infections.		1991	1899588
relative contributions of host and microbial factors in bacterial translocation.	to study the relative contributions of host and microbial factors in bacterial translocation, germfree mice were mono-associated with either proteus mirabilis, escherichia coli, or enterococcus faecalis. germfree mice included t-cell-deficient nude mice and normal littermates, natural killer cell-deficient beige mice and normal littermates, and triply immunodeficient mice with beige, t-cell, and b-cell mutations and their littermates. each bacterial species colonized the cecum in similarly high ...	1991	1899560
effect of staphylococcus epidermidis on adherence of pseudomonas aeruginosa and proteus mirabilis to polymethyl methacrylate (pmma) and gentamicin-containing pmma.	the goals of this study were to determine 1) effect of staphylococcus epidermidis adherence and biofilm production on adherence of the opportunistic pathogens proteus mirabilis and pseudomonas aeruginosa to polymethyl methacrylate (pmma); 2) if the biofilm killed by autoclaving altered adherence of other organisms; 3) if adherence of s. epidermidis to gentamicin-containing pmma altered adherence of the opportunistic pathogens p. mirabilis and p. aeruginosa to gentamicin-containing pmma. results ...	1991	1899449
diagnostic and genetic studies in 43 patients with classic cystinuria.	we present results for laboratory screening and diagnostic tests--cyanide-nitroprusside test, semi-quantitative thin-layer chromatography, and quantitative amino acid column chromatography--of 43 patients with classic cystinuria. we report the efficaciousness of the cyanide-nitroprusside test and of thin-layer chromatography, as compared with quantitative amino acid chromatography, for detecting heterozygotes for type ii or iii cystinuria. the quantitative results for aminoaciduria in 57 blood r ...	1991	1899064
flinders island spotted fever: a newly recognised endemic focus of tick typhus in bass strait. part 2. serological investigations.	twenty-six cases of a spotted-fever-like illness have been identified on flinders island, tasmania, over a 17 year period. these patients and 335 healthy persons from the island were investigated serologically using the weil-felix agglutination test (proteus sp. antigens ox2, ox19, oxk) and rickettsia-specific microimmunofluorescence. the antigens used in these latter tests comprised one member of the typhus group (rickettsia typhi) and three members of the spotted fever group (rickettsia ricket ...	1991	1898756
[the use of ampicillin/sulbactam (unasyn) in treating inflammatory urological diseases].	unasyn is a combination of ampicillin, a bactericidal antibiotic, and sulbactam, an inhibitor of beta-lactamases. it was used in treatment of 36 patients with urogenital infections. the combination was administered intravenously and in the main intramuscularly. the treatment course amounted to 7-10 days. the average daily dose was 6 to 9 g. 22 patients with acute nonocclusive pyelonephritis were treated with the combination and its clinical and bacteriological efficacy was stated in 95 per cent ...	1991	1898184
proteus mirabilis biofilm protection against struvite crystal dissolution and its implications in struvite urolithiasis.	proteus mirabilis biofilm formation, struvite (mgnh4po4.6h2o) crystal formation and dissolution in an artificial urine mixture were monitored using computer-enhanced microscopy (cem) and a 1 x 3 mm. glass flow cell. image analysis showed that p. mirabilis biofilm formation did not occur to any extent at macroenvironment flow rates greater than two ml/h (equivalent to a microenvironment flow rate of less than 5 microns./sec). essentially, cells attached to glass surfaces, grew slowly and divided. ...	1991	1895441
proteus syndrome. ultrastructural study of linear verrucous and depigmented nevi.	proteus syndrome is a rare hamartomatous disorder characterized by multifocal overgrowths that can involve any structure of the body. clinical manifestations include macrodactyly, hemihypertrophy, subcutaneous masses, exostosis, cerebroid thickening of palms and soles, and linear skin lesions. about 50 cases have been described, but the ultrastructural features of the linear skin lesions have not been characterized. we describe the clinical, histologic, and ultrastructural findings for a 30-year ...	1991	1894776
efficacy of a proteus mirabilis outer membrane protein vaccine in preventing experimental proteus pyelonephritis in a balb/c mouse model.	a balb/c mouse model of nonobstructive, ascending proteus mirabilis pyelonephritis was characterized bacteriologically, histologically, and serologically from 3 to 28 days. intravesicular administration of 2 x 10(8) p. mirabilis k7 resulted in the septic death of 9 (16%) of 57 mice by day 15. among the survivors, k7 colonized the kidneys in great numbers until day 21. histological examination of the kidneys revealed acute inflammation which was characterized by neutrophil infiltration by day 3, ...	1991	1894376
inhibition of acid secretion from parietal cells by non-human-infecting helicobacter species: a factor in colonization of gastric mucosa?	helicobacter pylori has been shown to produce a protein that inhibits acid secretion from parietal cells. we have examined other non-human-infecting helicobacter species for this property by measuring the uptake of [14c]aminopyrine into rabbit parietal cells as an indirect assessment of acid secretion. helicobacter felis and an isolate from a rhesus monkey were shown to inhibit acid secretion. isolates of helicobacter mustelae gave variable responses. whole bacteria and cell-free sonicates impai ...	1991	1894369
proteus mirabilis urease: use of a urea-lacz fusion demonstrates that induction is highly specific for urea.	proteus mirabilis, a common agent of nosocomially acquired and catheter-associated urinary tract infection, is the most frequent cause of infection-induced bladder and kidney stones. urease-catalyzed urea hydrolysis initiates stone formation in urine and can be inhibited by acetohydroxamic acid and other structural analogs of urea. since p. mirabilis urease is inducible with urea, there has been some concern that urease inhibitors actually induce urease during an active infection, thus compoundi ...	1991	1894350
evaluation of effects of novel urease inhibitor, n-(pivaloyl)glycinohydroxamic acid on the formation of an infection bladder stone using a newly designed urolithiasis model in rats.	by using our new infection stone model of a rat, we evaluated the effect of a novel urease inhibitor, n-(pivaloyl)glycinohydroxamic acid (p-gha), on the formation of an infection bladder stone. the oral dosing of p-gha significantly inhibited the elevation of the urinary ammonia level of rats having the urinary tract infection with proteus mirabilis. a short term regimen (7 d, 730 +/- 38 mg/kg) with p-gha significantly inhibited the development of the infection bladder stone. furthermore, a long ...	1991	1893494
[sodium sulfapyridazine in new delayed-action bases for the treatment of bacterial conjunctivitis].	possible preparation of 10 per cent sodium sulfapyridazine ophthalmic drops containing aubazidan, a microbial polysaccharide, providing prolongation of the drops action and stability on the storage was studied. the pharmacokinetic studies showed that aubazidan which is a natural polymer provided high prolongation of the sulfapyridazine effect in the tissues of the anterior part of the eye in rabbits. the prolonged effect was similar to the previously observed effect of the solutions containing s ...	1991	1892429
combination effect of sce-2787 and cefepime with aminoglycosides on nosocomial gram-negative bacteria.	the in vitro activity of cefepime and sce-2787, two new parenteral cephalosporins, and the combination effect with tobramycin and gentamicin against nosocomial gram-negative rods was studied using checkerboard agar dilution technique. cefepime showed excellent in-vitro activity against klebsiella pneumoniae, enterobacter cloacae, serratia marcescens and proteus vulgaris (mic90 0.03-0.125 mg/l) and good to moderate activity against acinetobacter anitratus, pseudomonas aeruginosa and pseudomonas c ...	1991	1889877
chronic suppurative otitis media: complicated versus uncomplicated disease.	to determine prognostic factors in patients with chronic otitis media who develop suppurative complications, we performed a retrospective study of all patients with chronic suppurative otitis media who underwent mastoid surgery between the years 1981 and 1989. patients who developed complications were compared with those who did not with respect to six prognostic variables. the results indicated that patients with complications were younger (p less than 0.001), had shorter duration of ear discha ...	1991	1887779
[the mutagenicity of a new multicomponent vaccine made from the antigens of klebsiella pneumoniae, staphylococcus aureus, proteus vulgaris and escherichia coli].	inactivated bacterial vaccine, containing k. pneumoniae, s. aureus, p. vulgaris and e. coli antigenic complexes were tested for mutagenicity in the test described by ames et al. and in vivo, in experiments on mice. in salmonella typhimurium cells, strain ta-98 and ta-100, the preparation (5-75 mg/ml) did not increase the frequency of reversions and histidine-independence either in direct experiments or after metabolic activation with rat liver homogenate. in experiments on mice the vaccine (3.3 ...	1991	1887711
in vitro and in vivo transferrable beta-lactam resistance due to a new plasmid-mediated oxyiminocephalosporinase from a clinical isolate of proteus mirabilis.	a new plasmid-mediated beta-lactamase (fpm-1) with an isoelectric point of 7.2 and a molecular weight of 26,000 was found in a cefuroxime-resistant clinical isolate of proteus mirabilis strain 6003. fpm-1 can be classified as a type i oxyimino-cephalosporinase on the basis of its substrate specificity and inhibition pattern by clavulanic acid etc., and its conferred resistance on both the strain and transconjugants against most oxyme-type cephalosporins as well as the older ones but not against ...	1991	1886493
[the proteus syndrome].		1991	1884412
clinical patterns of paediatric urolithiasis.	a series of 270 paediatric stone patients was studied retrospectively according to the clinical pattern of urolithiasis (age and sex, stone location, stone analysis, recurrence rate) and aetiology of stone disease (infection, anatomical, metabolic or idiopathic). infection stones occurred earliest and more commonly in males and were usually upper tract struvite calculi related to proteus infection. anatomical stones were most commonly associated with pelviureteric junction (puj) obstruction and ...	1991	1884149
[the specific activity of a multicomponent vaccine made from the antigens of opportunistic microorganisms].	multicomponent vaccine prepared from the antigens of 4 representatives of opportunistic microflora possesses high specific activity. the passive hemagglutination (pha) test with the use of associated diagnosticum showed that antibody titers in the sera of immunized rabbits increased 10- to 10(4)-fold in comparison with the titers observed prior to immunization. the pha test with the use of the antigens contained in the vaccine revealed the accumulation of antibodies to each of the 4 components o ...	1991	1882605
[frequency and antibiotic susceptibility of bacteria responsible for urinary infections in private practice from 1986 to 1990. results of 5398 samples].	for each year from 1986 to 1990, fourteen private laboratories from all over france collected epidemiological data on germs discovered in urinary infections in france. this retrospective and comparative study covers 5,398 dossiers. the sensitivity of germs in private practice in urinary infections (ampicillin, am-ac, cephalosporin, ceftriaxone, gentamicin, pipemidic acid, pefloxacin, cotrimoxazol) were analysed. germ resistance tests carried out by the cmi study in the centre of reference (pr th ...	1991	1881681
[enterobacteriaceae isolated in nine general hospitals of the ile-de-france in 1988 and 1989: susceptibility to beta-lactam antibiotics according to the length of hospitalisation].	susceptibility to beta-lactam antibiotics of strains of enterobacteriaceae consecutively isolated in nine general hospitals during a period of 2 months (march and april) has been studied by the disk-agar diffusion method. the separation between susceptible and resistant strains was based on the measure of the inhibition zones centered by 2 disks: cephalothin and ticarcillin. enterobacteriaceae were divided in 2 groups: strains isolated during the first 48 h of hospitalisation or isolated after. ...	1991	1881679
[in vitro sensitivity of gram-negative bacteria isolated from urinary tract infections to various antibiotics].	this study was done on the outpatients and hospitalized patients who were suffering from urinary tract infections in türkiye yüksek ihtisas hospital. 500 positive cultures were evaluated. e. coli was the predominant organism and it is followed by klebsiella and coliform bacteria. the sensitivity of the isolated bacteria were tested against various antibiotic discs using disc diffusion method. the sensitivity of the bacteria to the used antibiotics were as follows: ceftazidime 96%, ofloxacin 95.6 ...	1991	1881356
bacterial isolates and drug susceptibility tests at kaffa regional public health laboratory, south west ethiopia.	a two year period bacteriological data was analysed and the frequent bacterial isolates from different clinical specimens included: s. aureus, 25% e. coli, 15%; proteus spp 14%; citrobacter-enterobacter group, 10% coagulase negative staphylococcus species, 9%; and other miscellaneous bacteria each less than 9%. the majority of the bacterial isolates were resistant to commonly available antimicrobial agents like tetracycline, ampicillin, and chloramphenicol.	1991	1880409
treatment of staghorn calculi with extracorporeal shock-wave lithotripsy and percutaneous nephrolithotomy.	between august 1983 and august 1987, 72 staghorn calculi were treated in 66 patients. treatment was with percutaneous nephrolithotomy (pcnl) in 30, extracorporeal shock-wave lithotripsy (eswl) in 18, combination pcnl-eswl in 23, and nephrectomy in 1. complications occurred in 59 percent of patients and were twice as common after pcnl as after eswl. radiologic follow-up on 69 kidneys (97%) showed 58 percent were stone-free, 15 percent had residual sand or matchheads less than 5 mm, 17 percent had ...	1991	1877132
bacteremia during extracorporeal shock wave lithotripsy of renal calculi.	an increasing incidence of bacterial endocarditis has been observed since the beginning of the last decade. an explanation for this fact is the expansion of diagnostic and therapeutic procedures in diverse medical disciplines. in a clinical study performed on 49 consecutive patients undergoing extracorporeal shock wave lithotripsy a 14.3% rate of bacteremia was detected during treatment. the bacterial spectrum consisted of gram-positive cocci, gram-negative cocci, bacteroides capillosus and prot ...	1991	1875482
extracorporeal shock wave lithotripsy for struvite renal calculi: prospective study with extended followup.	to clarify the significance of retained stone particles after extracorporeal shock wave lithotripsy (eswl) for struvite renal calculi we followed 22 otherwise healthy women for 16 to 52 months (mean 39 months). each patient had persistent proteus mirabilis bacteriuria before eswl and received a standardized regimen of antimicrobial therapy in the perioperative period only. of the 22 patients 19 (86%) were cured of the persistent bacteriuria. of these 19 patients 16 had retained stone particles a ...	1991	1875481
[diabetic foot infections: a preliminary report].	foot infection is a common cause of hospitalization for diabetic patients. our study aimed to analyze patients' age, sex, the primary sites of infection, presenting features, outcome, bacteriologic studies and their clinical significance in diabetic foot infections. the age of our diabetic patients with foot infections ranged from 43 to 82 years, with a mean of 62.5 years; fifty-two of them were males and twenty-eight were females. the male to female ratio was 1.9:1, but there was no statistical ...	1991	1875459
an improved selective medium for the isolation of escherichia coli o157.	sorbitol-macconkey medium has become widely used for the isolation of verotoxigenic (vt+) escherichia coli o157. however, many organisms other than vt+ e. coli o157, especially other serogroups of e. coli and proteus spp., may not ferment sorbitol, and thus may be confused initially with vt+ e. coli o157. rhamnose is not fermented by vt+ e. coli o157, but is by most sorbitol non-fermenting e. coli of other serogroups. cefixime is a cephalosporin antibiotic that is more active against proteus spp ...	1991	1875390
intravenous ciprofloxacin for the treatment of severe infections.	intravenous ciprofloxacin at a daily dosage of 400 mg divided in two doses was administered to 19 patients with severe infections caused by ciprofloxacin-susceptible bacteria. these infections included: 11 surgical would infections, 5 soft tissue infections, 2 respiratory tract infections, 1 urinary tract infection. the offending pathogens were: 8 coagulase-negative staphylococci, 3 staphylococcus aureus, 3 pseudomonas aeruginosa, 2 proteus spp., 1 escherichia coli, 1 branhamella catarrhalis, 1 ...	1991	1875231
in vitro activity of an oxycephem ocp 9-176 compared with its sulfur analog and other beta-lactams.	ocp 9-176 is an oxycephem antibiotic that contains a 2-aminothiazolyl, carboxypropyl side chain at c-7 and a pyridinium thiomethyl group at c-3. ocp 9-176 was generally twofold less active than its sulfur-containing analog me 1228 against escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, providencia stuartii, proteus vulgaris, and serratia marcescens. activity against enterobacter cloacae and citrobacter freundii was equivalent. ocp 9-176 was twofold less active than me-1228 against p ...	1991	1873972
anaesthesia for proteus syndrome.	a 14-year-old boy with proteus syndrome presented for orthopaedic surgery to his legs. no report in the literature exists on anaesthesia for this condition. our patients posed airway problems that were managed by tracheal intubation under sedation using a fibreoptic bronchoscope. anaesthesia was induced with thiopentone and maintained with nitrous oxide and isoflurane in oxygen. his lungs were ventilated mechanically throughout surgery, which was uneventful.	1991	1872427
intermittent urethral self-catheterisation: long term results (bacteriological evolution, continence, acceptance, complications).	forty six (9%) out of 520 patients from the years 1976 to 1982 who used intermittent urethral self-catheterisation (iusc) as the method of bladder drainage at the moment of discharge from the swiss paraplegic centre of basel were analysed during their rehabilitation period, on discharge, 3, 6 months later and again 5 years later regarding bacteriological changes and urinary tract infections, behaviour of some problem germs such as proteus, klebsiella and pseudomonas, sterile versus clean cathete ...	1991	1870888
[prenatal diagnosis of abdomino-pelvic cystic lymphangioma as part of proteus syndrome].	proteus syndrome is difficult to diagnose owing to its low incidence and to the late recognition of its identity, due to polymorphous border diseases and to the polymorphous lesions which characterize. the authors report a prenatal diagnosis of abdominal and pelvic cystic lymphangioma as part of proteus syndrome.	1991	1869778
[renal abscess. medical treatment].	we report a case of renal abscess caused by gram negative pathogens, possibly proteus mirabilis, that resolved with medical treatment. the infection was secondary to a purulent infection in the patient's left knee.	1991	1867511
intestinal colonization with enterobacteriaceae in pakistani and swedish hospital-delivered infants.	rectal cultures from swedish and pakistani hospital-delivered newborn infants were analysed regarding the early acquisition of enterobacteria. swedish infants were delivered vaginally, pakistani infants were delivered either vaginally or by caesarean section. the swedish infants were all breast-fed, whereas breastfeeding was incomplete and often started late among the pakistani infants. both groups of pakistani infants were more rapidly colonized with enterobacteria than were the swedish infants ...	1991	1867075
[dynamic anti-proteus immunity in plasma donors during plasmapheresis].	a study is presented of homeostasis in 18 volunteer donors after immunisation with proteus vaccine and in the dynamics of subsequent plasmapheresis for 12 months. it was shown that as a result of anti-proteus immunisation there occurs an increase of the titer of anti-proteus antibodies in the blood serum which was maintained at a high level in the course of plasmapheresis for three months. indices of the peripheral blood, urine, liver function, humoral unspecific and cellular immunity were withi ...	1991	1866911
biofilm and scleral buckle-associated infections. a mechanism for persistence.	scleral buckle infections tend to be persistent as well as resistant to antimicrobial treatment. often, scleral buckle infections require removal of the buckling elements for resolution. to determine if bacteria are able to persist on scleral buckles by elaborating a glycocalyx matrix or biofilm that offers protection against host defenses and antimicrobial treatment, the authors cultured 28 scleral buckle elements removed for infection and extrusion. bacteria were isolated from 18 elements (64% ...	1991	1866148
microbiological status of egyptian prawn.	a total of 110 prawn individuals were collected from different markets at cairo and giza governorates. the average counts of aerobes, psychrophiles, enterobacteriaceae, coliforms and staphylococci were 5 x 10(3), 3 x 10(2), less than 2 x 10(2), less than 3 and 2 x 10(2) organisms per gram newly caught prawn sample, respectively. such counts were significantly increased in samples collected from markets including raw prawn in shell, raw peeled samples and frozen samples either peeled or in shell. ...	1991	1865888
use of chromatofocusing for separation of beta-lactamases. ix. analytical chromatofocusing for the separation of a chromosomal cephalosporinase from proteus vulgaris 1028.	simultaneous purification and isoelectric point (pi) determination was carried out at analytical scale of the chromosomal cephalosporinase from the proteus vulgaris 1028 strain. comparison of the enzyme to the purification results with m-aminophenylboronic acid-agarose affinity chromatography with sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that minute amounts of accompanying proteins having identical pi values but different molecular masses were found in the chromatofocu ...	1991	1864899
mode of antibacterial action of cefprozil, a new cephalosporin, on escherichia coli, serratia marcescens and morganella morganii.	the mode of antibacterial action of cefprozil (cfpz, bmy-28100), a newly developed cephalosporin, was investigated using escherichia coli k12, serratia marcescens ifo 12648 and morganella morganii ifo 3848 as test organisms, in comparison with the action of cefaclor (ccl). the minimum inhibitory concentrations (mics) of cfpz for these organisms were 1.56, 800 and 25 micrograms/ml, whereas those of ccl were 1.56, 800 and 100 micrograms/ml, respectively. the addition of a subinhibitory concentrati ...	1991	1864765
gentamycin beads in the treatment of localised vascular graft infection--long term results in 17 cases.	seventeen patients were treated by the implantation of gentamycin beads for localised szilagyi type iii prosthetic graft infection. three patients had a pan prosthetic infection and half of the remaining patients were cured, but three of these died from other causes within 3 months. the mean observation time of the four survivors was 20 months (7-39 months). one of the seven patients, in whom the gentamycin beads failed, died of infection, giving a total mortality rate of 6% (1.5-29%). the remai ...	1991	1864394
epidemiology of beta-lactamases in africa: correlation with resistance to beta-lactam antibiotics.	in 45 centers from eight african countries, 2,888 bacterial isolates were collected from patients with community-acquired infections. isolated pathogens included staphylococcus aureus (29%), escherichia coli (20%), neisseria gonorrhoeae (6%), proteus species (6%), klebsiella species (6%), klebsiella pneumoniae (4%), and staphylococcus epidermidis (4%). an overall sensitivity of 16.2% was shown to penicillin g (number of isolates tested = 2,467), 31.8% to ampicillin (2,687), 45% to amoxicillin (1 ...	1991	1863940
[induced bacterial chemiluminescence].	oxygen-peroxide-induced superweak luminescence of some microorganisms has been studied by means of the native chemiluminometer khlm1ts-01. it is established that total chemiluminescence decrease in the bacteria as follows: escherichia coli----bacillus subtilis----proteus vulgaris----bacillus mycoides----b. licheniformis----pseudomonas aeruginosa----staphylococcus epidermidis----s. aureus----serratia marcescens----salmonella enteritidis. a hypothesis is put forward on a possible mechanisms of app ...	1991	1861655
suppression and recovery of the alveolar macrophage phagocytic system during continuous exposure to 0.5 ppm ozone.	short-term exposures to ozone (o3) are known to impair pulmonary antibacterial defenses and alveolar macrophage (am) phagocytosis in a dose-related manner. to determine the effect of prolonged o3 exposure, swiss mice were exposed continuously to 0.5 ppm o3. at 1, 3, 7, and 14 days, intrapulmonary killing was assessed by inhalation challenge with staphylococcus aureus or proteus mirabilis and by comparing the number of viable bacteria remaining in the lungs at 4 h between o3-exposed and control a ...	1991	1860453
bacterial flora of the uterus of cows after calving on two hygienically contrasting farms.	intrauterine swabs were obtained from cows after calving on two commercial dairy herds with contrasting hygienic environments and incidence of leucorrhea, and cultured aerobically and anaerobically. of 26 cows with a normal calving and puerperium, eight of 14 (57 per cent) were sterile on farm b where hygiene was poor, compared with five of 12 (42 per cent) on farm a where hygiene was better. two cows on farm b retained their placentas and subsequently developed metritis/endometritis. actinomyce ...	1991	1858270
characterization of the helicobacter pylori urease and purification of its subunits.	helicobacter pylori (formerly campylobacter pylori) is the causative agent of gastritis in man. helicobacter pylori cells contain a large amount of an extremely active urease (e.c.3.5.1.5). this enzyme is suspected to be a virulence factor since the ammonium ion produced from urea may be responsible for tissue injury and/or survival of h. pylori in the gastric environment. helicobacter pylori urease, native relative molecular mass approximately 600,000, was purified by agarose gel filtration and ...	1991	1857197
in vitro synthesis and o acetylation of peptidoglycan by permeabilized cells of proteus mirabilis.	the synthesis and o acetylation in vitro of peptidoglycan by proteus mirabilis was studied in microorganisms made permeable to specifically radiolabelled nucleotide precursors by treatment with either diethyl ether or toluene. optimum synthesis occurred with cells permeabilized by 1% (vol/vol) toluene in 30 mm mgcl2 in in vitro experiments with 50 mm tris-hcl buffer (ph 6.80). acetate recovered by mild base hydrolysis from sodium dodecyl sulfate-insoluble peptidoglycan synthesized in the presenc ...	1991	1856164
antimicrobial effects of lomefloxacin in vitro.	the mic of lomefloxacin was determined for 554 isolates from the urinary tract. some of the more resistant strains of escherichia coli, proteus mirabilis, klebsiella pneumoniae, and pseudomonas aeruginosa were studied in a dynamic in-vitro model in order to study dosing strategies. the model simulated, in mueller-hinton broth, the profile of plasma lomefloxacin concentrations in volunteers. bacteria were exposed to a range of lomefloxacin concentration profiles achievable by oral dosing. the fir ...	1991	1856127
bacteriology of the anal wound after open hemorrhoidectomy. qualitative and quantitative analysis.	the purpose of this study is to analyze the size of the bacterial colonies in anal wounds after open hemorrhoidectomy. twenty patients were studied during predetermined postoperative time periods. material was collected from the surface and from within the tissue of each patient's three open wounds, intraoperatively, on the 6th, 13th and 20th postoperative days for bacteriologic examination in aerobic, microaerophilic, and anaerobic media. the bacterium most commonly identified was escherichia c ...	1991	1855423
[pediatric bacteremia strains grow in blood culture media].	bacteremia strains may influence the clinical therapy, the isolation of bacteremia strains is important for patients. in order to understand the distribution and the growth in the blood culture media of bacteremia strains, from january 1982 through december 1988 we studied the blood culture sent to us from the pediatric ward. these were three hundred and forty one bacterial strains (3.1%) isolated from patients. in the meantime we also studied the bacteremia strains growth in the blood culture m ...	1991	1848461
prenatal sonographic findings in a fetus with proteus syndrome.		1991	1847725
[bacterial translocation in a model of intestinal obstruction. ii. bacteriological study and role of cellular immunity].	bacterial translocation (bt) from the gastrointestinal (gi) tract to systemic organs creates the possibility of infection and sepsis in a great number of pathologic entities. in a mouse model of intestinal obstruction (io), we evaluated the type of micro-organisms and the organs that bacteria frequent translocated. at 24 hours post-10, positive cultures where obtained at the mln, portal, systemic circulation and peritoneal cavity, establishing that the translocation is bi-directional. the more f ...	1991	1843960
bacterial swarming: an example of prokaryotic differentiation and multicellular behaviour.	bacterial swarming involves the differentiation of vegetative cells into hyperflagellated swarm cells which undergo cycles of rapid and coordinated population migration across solid surfaces. species capable of this simple form of developmental behaviour lie on the boundary between unicellular and multicellular organisms and provide processes for study which are not only of intrinsic interest but which are analogous to components of more complex eukaryotic systems. this review attempts to place ...	1991	1842857
effect of the fungicides tridemorph and vinclozolin on soil microorganisms and nitrogen metabolism.	the effects of tridemorph and vinclozolin were studied on different types of microorganisms, urea hydrolysis and nitrification in soil and in culture. the fungicides adversely affected the population of bacteria, fungi and actinomycetes as a function of time of incubation. urea hydrolysis both in culture and soil were also inhibited by the fungicides, and tridemorph was more detrimental. in soil, 45 micrograms/g of tridemorph inhibited 50% of ammonification of urea, id50 for nitrite production w ...	1991	1841872
nucleotide sequence and characterization of a carbenicillin-hydrolyzing penicillinase gene from proteus mirabilis.	the structural gene of a carbenicillinase was cloned from the chromosomal dna of proteus mirabilis gn79. this gene codes for a protein of 270 amino acids. alignment of the amino acid sequence with those of known beta-lactamases revealed that the enzyme is a novel class a beta-lactamase with a unique conserved triad, rtg. by using a dna fragment of the structural gene, a lack of cross hybridization was confirmed between the dna probe and total dnas from natural isolates of p. mirabilis, suggestin ...	1991	1840585
dna fingerprinting: the utilization of minisatellite probes to detect a somatic mutation in the proteus syndrome.	syndromes with localized or segmental abnormalities have been proposed to be the result of a somatic mutation leading to the presence of somatic mosaicism in the tissue. the proteus syndrome, with its hemihypertrophy, macrodactyly and exostoses, has features which would indicate that the phenotype results from such events. the success of utilizing dna fingerprint probes to detect somatic mutations in cancer raised the possibility that a similar approach might be successful in an investigation of ...	1991	1831169
[classification of sensitive and resistant strains based on the degree of antibiotic growth inhibition].	the course of growth suppression of sensitive strains depends on the type of antibiotic. based on the kinetics of the early growth phase in the microculture when the inoculum is relatively concentrated it is possible to divide in the presence of the antibiotic the gram negative fermenting rods into sensitive and resistant strains already within five hours. data obtained by automated photometric assessment at selected intervals were processed by the aggregation method using a block distance as th ...	1991	1828715
species incidence and antimicrobial-agent resistance patterns of enterobacteriaceae in charles nicolle hospital, tunis, from 1983 to 1987.	between july 1983 and december 1987, 13,108 strains of enterobacteriaceae were isolated at charles nicolle hospital in tunis. this study reports the prevalence of different species isolated, their resistance and the evolution of bacterial resistance during that period. there appeared to be a great stability in the distribution of bacterial groups. among the commonly sensitive species, proteus mirabilis showed a high proportion of strains resistant to ampicillin (79.3%), carbenicillin (75.9%), ce ...	1991	1823087
[the genus proteus: microbiological and clinical aspects].		1991	1822710
[vibrio alginolyticus and swimmer's otitis externa. 2 cases and review of the literature].	we describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the mediterranean shore, with vibrio alginolyticus recovered from ear fluid. we describe the biochemical profile and sensitivity pattern (mic) of both strains, comparing it to previously published data. a literature review was also performed, in which we found evidence for increasing concern of v. alginolyticus as an human pathogen. also there is a need for considering halophilic vibrio as potential pathog ...	1991	1822155
[the characteristics of the initial link in the intestinal lesion by opportunistic proteus mirabilis bacteria].	interaction of proteus mirabilis with the intestine epithelium in white mongrel mice has been studied by means of bacteriological, toxicological and electron microscopic methods. introduction of the agar culture of bacteria to the intestine lumen has permitted finding its general toxic action on enterocytes and other cell elements of epithelium which was accompanied by a potentiation of the striated margin membrane degradation but caused no destruction of cells. a damaging factor of protei was c ...	1991	1819759
a comparative study on aztreonam, ceftazidime and amikacin in the treatment of complicated urinary tract infections.	in a prospective, randomized trial, aztreonam (1 g intravenously or intramuscularly, twice daily) was compared with ceftazidime (1 g intravenously or intramuscularly, twice daily) and amikacin (500 mg intravenously or intramuscularly, twice daily) in 76 patients aged 24 to 84 years (mean, 59.7 years) with complicated urinary tract infections. initial pathogens included escherichia coli (47.5%), pseudomonas aeruginosa (22.5%), klebsiella spp. (9%), proteus spp. (7.5%) and enterobacter spp (6%). i ...	1991	1819621
[neonatal septicemia and meningitis caused by gram-negative bacilli in yaondé: clinical, bacteriological and prognostic aspects].	based on 139 cases collected in 36 months, the authors show that neonatal sepsis and meningitis with gram negative bacilli represent 62.33% of confirmed neonatal sepsis (223 cases out of 6,784 neonatal hospitalisations). 53.96% of cases are manifest within the first 72 hours of life. 35.25% of these infants are preemies. the principal predisposing factors are: foul-smelling leukorrhea (45.23%), prolonged rupture of membranes (26.62%) and notion of resuscitation (36.69%). the most important clini ...	1991	1819407
antibacterial activities of amoxicillin alone and in combination with clavulanic acid correlated with beta-lactamase production.	amoxicillin, a beta-lactam antibiotic, was tested for its effect in combination with clavulanic acid, a beta-lactamase inhibitor, against 9 species of bacteria isolated from clinical specimens. a total of 698 strains of bacteria were tested for beta-lactamase production by the rapid chromogenic cephalosporin method. their susceptibilities to amoxicillin alone and in combination with clavulanic acid were tested by the agar dilution method. the percentage of beta-lactamase producing strains ranged ...	1991	1818798
pathogenic escherichia coli o157:h7 and their detection.	enterohaemorrhagic escherichia coli o157:h7 is of major concern to the food industry due to high pathogenicity of this foodborne organism. for the detection of these bacteria a special agar medium with a fluorogenic substrate has been developed. the medium uses the characteristics of this e. coli serotype not to ferment sorbitol and not to produce beta-glucuronidase. in contrast, approximately 96% of all other strains of e. coli are sorbitol-positive and nearly all of them are beta-glucuronidase ...	1991	1817428
bacteriology of community acquired urinary tract infection. analysis of 1048 cases.	urine samples from 1048 cases clinically suspected to have urinary tract infection were examined for bacteriological culture and antibiotic sensitivity. of 1048 cultures, 530 (50.5%) were sterile, 163 (15.5%) showed the presence of nonpathogenic bacteria and 11 (1%) revealed nonsignificant bacteriuria with escherichia coli. significant bacteriuria (more than 10(5)/ml) was found in the remaining 344 (32.8%) cases. escherichia coli was the predominant pathogen isolated from 170 (16.2%) samples; kl ...	1991	1814873
clinical results of a multicenter study with sulbactam/ampicillin for the treatment of patients with lower respiratory and urinary tract infections.	a total of 124 patients with lower respiratory tract (44) or urinary tract infections (80) were enrolled in an open, multicenter study to evaluate the efficacy and tolerability of sulbactam/ampicillin, administered at the dosage of 3 g/die by intramuscular route. pretreatment pathogens from patients with lower respiratory tract infections included: streptococcus alpha-haemolyticus in 8 cases, streptococcus beta-haemolyticus in 2 cases, staphylococcus albus in 7 cases, haemophilus influenzae in 7 ...	1991	1809811
the incidence and beta-lactam resistance of proteus vulgaris in hospital infections: the last decade.	during the period of 1980-1990, 581 proteus vulgaris strains were obtained in a general hospital. they were considered as the significant isolate in 0.6% of soft tissue infections, 0.6% of urinary tract infections and in 0.2% of bacteremic episodes. sixty-three percent of the 393 tested strains showed resistance to ampicillin, cefazolin and cefamandole or cefuroxime. about 7% were susceptible to all beta-lactam drugs, and showed a very low beta-lactamase activity and 5% of the strains showed a p ...	1991	1809807
[the genus proteus. pathogenic and epidemiologic aspects].		1991	1805954
what is the diagnostic value of beta-d-glucuronidase (bdg) activity of bacteria using fluorocult ecd agar for their cultivation?	a total of 1510 strains from 15 genera of gram-negative and gram-positive bacteria were studied. more than 94% of 327 escherichia coli strains showed beta-d-glucuronidase (bdg) activity. seventeen serotypes from 170 e. coli o serogroup representatives were negative. relationship between the existence of bdg positive and negative e. coli strains in the same serogroup or serotype has not been observed. the rate of bdg positivity was 42% among salmonella arizonae strains and 42.2% among shigella st ...	1991	1805501
[long ureteral ammonium-magnesium phosphate (struvite) and calcium phospho-carbonate calculi].	the authors report about 12 cases of long ureteral calculi, 16 to 39 mm in size, observed over 10 years. they were all made of a mixture of ammonium-magnesium phosphate and calcium phosphocarbonate. infection was the revealing symptom, either in the form of simple bacteriuria or as acute pyelonephritis or sepsis. these calculi, found in a lumbar or pelvic location, were very long, radiopaque but with a moderate radiological density, homogeneous and have regular contours. they were straight, some ...	1991	1804876
acute anterior uveitis, arthritides and enteric antigens.	one hundred and fourteen patients with acute anterior uveitis were studied for the presence of the hla-b27 tissue type, the prevalence of spondylitis and arthritis and the occurrence of gastro-intestinal and urogenital infections or diarrhoeal illness in the history. eighty-seven (76%) were b27+ and 27 (24%) b27-. forty-two (48%) of the b27+ group had ankylosing spondylitis (as); 13 (30%) of them were females. sacroilitis (si) with no spinal involvement was present in 21 patients (24%), 13 (61%) ...	1991	1802494
[the effect of immunotherapy with cell-free vaccines made from the antigens of opportunistic microorganisms on the dynamics of dna antibody formation].	the sera of patients subjected to immunotherapy with staphylococcal vaccine and with multicomponent vaccine (i.e. the mixture of the antigenic preparations of staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris, escherichia coli) were studied by the method of the enzyme immunoassay on the basis of cattle spleen dna. immunotherapy with staphylococcal vaccine was given to patients with dermal diseases, chronic obstructive bronchitis and pulmonary abscess. multicomponent vaccine was intr ...	1991	1801489
[the immunogenicity of mono- and associated vaccines made from strains of opportunistic bacteria].	in experiments on white mice the immunogenic potency of two variants of combined vaccines prepared from strains of opportunistic bacteria was compared with that of monovaccines used as the components of the combined vaccines. all the vaccines under study were found highly immunogenic. decrease of monovaccine share in the combined vaccine did not reduce the immunogenic potency to any of the strains. an 8- to 16-fold increase in the level of specific antibodies in the colostric lactosera of cows i ...	1991	1801485
[the characteristics of the contact of proteolytic strains of proteus mirabilis and klebsiella pneumoniae with enterocytes in vivo].	the relationships of opportunistic p. mirabilis and k. pneumoniae, both having proteolytic properties, with enteric cells in white mice have been studied on a ligated intestinal loop, used as an experimental model, by electron microscopy. this investigation has shown that the strains under study are capable of damaging the membrane of the jagged edge of enteric cells at the distance of the spread of proteolytic metabolites. this damage is reversible, presenting as compensatory protective reactio ...	1991	1801481
cefpodoxime: comparative antibacterial activity, influence of growth conditions, and bactericidal activity.	the antimicrobial activity of cefpodoxime, the active metabolite of the new cephalosporin ester cefpodoxime proxetil, in comparison to cefixime, cefotiam, cefuroxime, and cefotaxime was determined against a broad spectrum of freshly isolated gram-positive and gram-negative bacterial strains. cefpodoxime was demonstrated to be inhibitory at concentrations of less than or equal to 1 mg/l against 90% of strains of moraxella catarrhalis, haemophilus influenzae, escherichia coli (beta-lactamase- nega ...	1991	1800379
antibacterial activity of cefpodoxime in comparison with cefixime, cefdinir, cefetamet, ceftibuten, loracarbef, cefprozil, bay 3522, cefuroxime, cefaclor and cefadroxil.	the new oral cephalosporins cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten demonstrate enhanced activity against enterobacteriaceae susceptible to the established compounds as well (e.g. cefuroxime, cefaclor, cefadroxil). in addition, cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten include in their spectrum species hitherto resistant to oral cephalosporins (proteus vulgaris, providencia spp., yersinia enterocolitica). besides, the majority of these compounds demonstrate rele ...	1991	1800377
[the genus proteus. pathogenic and epidemiologic aspects].		1991	1799614
epilepsy following brain abscess. the evaluation of possible risk factors with emphasis on new concept of epileptic focus formation.	the study is based on follow-up data of 108 patients treated for brain abscess (mean follow-up period 11 years). the overall incidence of epilepsy was 34% (37% in supratentorial lesions). the author compared the incidence of several parameters between two groups of patients: those presenting, and those not presenting with epilepsy after brain abscess treatment. parameters characterized by significantly different incidence in each group were taken into account as possible risk factors influencing ...	1991	1799152
[susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates].	we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ...	1991	1798067
otitis media in sudanese children: presentation and bacteriology.	two hundred sudanese children (105 males, 95 females) with otitis media (om) were studied. their age range was from 3 months to 15 years, with 41.5% being below 2 years of age. the presenting symptoms included ear discharge in 96% and pyrexia in 26.5% of patients. ear ache and itching were reported in 22.5% and 7.0% respectively and impaired hearing in 7.0%. bacterial pathogens were isolated from 115 (70.1%) of 164 cultures from children with chronic discharging ears. organisms isolated in order ...	1991	1797530
in vitro activity evaluations of cefdinir (fk482, ci-983, and pd134393). a novel orally administered cephalosporin.	cefdinir, a so-called third-generation oral cephalosporin was tested in vitro against over 700 pathogens from patients with bacteremia. cefdinir was very active against the enterobacteriaceae with a 50% minimum inhibitory concentration (mic50) value range of less than or equal to 0.03-8 micrograms/ml. the enteric species having the highest mic90s (greater than or equal to 16 micrograms/ml) were citrobacter freundii, and the enterobacters, morganella morganii, proteus vulgaris, and serratia marce ...	1991	1797457
in vitro activity of a new cephalosporin me-1206 compared with other agents.	the in vitro activity of me-1206, a new aminothiazolyl cephalosporin that can be orally absorbed when converted to an ester, was compared with that of other beta-lactams. me-1206 inhibited 50% of the enterobacteriaceae at 2 micrograms/ml, similar to cefotaxime, ceftazidime, and cefixime. it did not inhibit, mic greater than or equal to 32 micrograms/ml, enterobacter species or citrobacter freundii resistant to cefotaxime and ceftazidime, and it was less active than cefotaxime and ceftazidime aga ...	1991	1797456
the elephant man.		1991	1795079
synthesis and biological activity of pyrimido[2,1-b][1,3]thiazine, [1,3]thiazino[3,2-a]purine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives and thiazole analogues.	some series of thiazolo[3,2-a]pyrimidine, pyrimido[2,1-b] [1,3]thiazine, thiazolo[3,2-a]purine, [1,3]thiazino[3,2-a]purine, thiazolo[3,2-a][1,2,3]triazolo[4,5-d]pyrimidine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives, variously functionalized, were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely: e. coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, s. faecalis, ...	1991	1793474
[taxonomic characteristics of soil mycobacteria and their antibiotic properties].	morphological, cultural and chemotaxonomic properties of 12 gram-positive soil cultures isolated were studied by using a test system developed for screening the organisms producing broad-spectrum antibiotics among nocardiaforms (coryneforms). the cultures were found to belong to actinomycetales, nocardioforms, mycobacteriaceae and mycobacterium. the saprophytic rapidly growing soil mycobacteria showed antibiotic activity against a large number of gram-positive and gram-negative test microbes inc ...	1991	1793300
[prolonged antibacterial action of polymer coated suture materials].	the antimicrobial activity of capromed, a surgical polymer-coated sutural material containing dioxidine, quinoxidine or gentamicin was studied in vitro and in vivo. capromed was shown to be active against the hospital strains of s. aureus, e. coli, proteus spp., klebsiella spp. and p. aeruginosa. the antimicrobial activity of adh and ag threads was preserved for 3 to 4 days after implantation. the dh-2 and g-2 threads preserved their activity for 6 to 7 days. it was concluded that the duration o ...	1991	1793296
[quantitation of acinetobacter calcoaceticus in mixed bacterial cultures by an enzyme immunoassay].	an enzyme-linked immunosorbent assay using polyclonal antibodies from rabbits has been developed for quantification of acinetobacter calcoaceticus. bacteria were added to the wells of a microtiter plate coated with anti-acinetobacter immunoglobulin. for detecting bound cells the peroxidase-labelled immunoglobulin fraction was used. over a distinct range there is a linear correlation between bound bacteria and measured absorbance allowing a quantification of bacteria in an order from 10(7) to 10( ...	1991	1793089