Publications

phylogenetic relationships of the soybean sudden death syndrome pathogen fusarium solani f. sp. phaseoli inferred from rdna sequence data and pcr primers for its identification.	phylogenetic relationships of several species within the fusarium solani-complex were investigated using characters from the nuclear ribosomal dna. genetic variation within 24 isolates, including 5 soybean sudden death syndrome (sds) strains, was assessed using rdna sequence data and restriction fragment length polymorphic markers. by these techniques, the causal agent of soybean sds was identified as f. solani f. sp. phaseoli. in separate cladistic analyses, plectosphaerella cucumerina and nect ...	1995	7579615
triglyceride hydrolysis and stability of a recombinant cutinase from fusarium solani in aot-iso-octane reversed micelles.	a recombinant cutinase from fusarium solani was encapsulated in aot reversed micelles. physicochemical parameters of the system were optimized relative to triolein hydrolysis. kinetic studies of triglyceride hydrolysis showed a decrease in specificity with increase of the acyl chain length. stability of cutinase in the system under study is lower than in aqueous solution and decreases with increase in the water content in the system (w0 = [h2o]/[aot]). the products of triolein hydrolysis had lit ...	1995	7535515
pathogenic effects of fusarium sulphureum, fusarium solani var. coeruleum and dry rot affected potatoes on the internal organs of rats.	rats of the wistar race were used in toxicological experiments involving fusarium sulphureum schl., f. solani var. coeruleum (sacc.) booth and potatoes damaged by these fungi. the in vivo and postmortem studies revealed that both fungi and effected tubers had hepatotoxic and nephrotoxic effects on the animals. morphological changes in the internal organs were mainly manifested by disturbances in blood circulation and regressive metamorphosis. these changes intensified proportionally to the dose ...	1993	7504875
phosphonate analogues of triacylglycerols are potent inhibitors of lipase.	1,2-dioctylcarbamoylglycero-3-o-p-nitrophenyl alkylphosphonates, with alkyl being methyl or octyl, were synthesised and tested as irreversible inhibitors of cutinase from fusarium solani pisi and staphylococcus hyicus lipase. rapid inactivation of these enzymes occurred with a concomitant release of one mole of p-nitrophenol per mole of enzyme. with both lipases a higher reactivity was observed when the alkyl substituent on the phosphonate is a methyl rather than an octyl chain. both lipases are ...	1995	7492616
cloning of a new pectate lyase gene pelc from fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of the gene product expressed in pichia pastoris.	antibodies prepared against a pectate lyase (pla) produced by a phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating type vi) were previously found to protect the host against infection. the cdna and gene (pela) for pla were cloned and sequenced. a new pectate lyase gene, pelc, was isolated from a genomic library of f. solani pisi with pela cdna as a probe. a 1.3-kb dna fragment containing the pelc gene and its flanking regions was identified and sequenced. the coding ...	1995	7487098
a case of mycotic keratitis caused by fusarium solani.	a 36-year-old black man, without history of systemic disease or ocular trauma developed a corneal infection in his left eye. he was treated with antibacterial antibiotic and corticosteroids for one month prior to diagnosis. fungal hyphae and chlamydospores were found in a koh preparation of the corneal scrapings, and positive cultures for fusarium solani were obtained in sabouraud dextrose agar. it is emphasized the cautious use of antibiotics and steroids in corneal diseases, and the need of co ...	1995	7481475
fusarium solani infection of a hypertensive leg ulcer in a diabetic.		1980	7464854
[levanase from fusarium solani-68].	extracellular levanase was isolated from the culture of fusarium solani-68 by ammonium sulfate precipitation at 0.8 saturation and purified in part by sephadex g-150 gel chromatography. levanase showed maximum activity at ph = 6.0 and t = 45 degrees c. the levanase reaction (hydrolysis of high molecular levane with a molecular weight of 1--5 min) reached a maximum rate at a polysaccharide concentration of 6.7 mg/ml, michaelis constant being 1.5 x 10(-6) m. levanase remained stable at ph 5.4--7.5 ...	1980	7433428
structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the n terminus.	cutinase i and cutinase ii, two extracellular enzymes produced by fusarium solani pisi, were shown to be glycoproteins containing 4.3% and 5.1% carbohydrates, respectively. upon treatment with alkali both enzymes generated chromophores which absorbed at 241 nm. treatment of both proteins with alkaline nab3h4 gave labeled protein and labeled monosaccharides. hydrolysis of the labeled protein followed by chromatographic and enzymatic analyses of the products showed that alanine, 2-aminobutyrate, p ...	1980	7398618
fusarium solani keratitis. first report from malaysia.		1981	7343825
mechanics of chromosome separation during mitosis in fusarium (fungi imperfecti): new evidence from ultrastructural and laser microbeam experiments.	the anaphase-telophase spindle usually elongates, and it has been assumed that the spindle pushes the incipient daughter nuclei apart. to test this assumption, we used a laser microbeam to sever the central spindle of the fungus, fusarium solani, and measured the rate of separation of incipient daughter nuclei. when the microbeam was aimed beside the spindle separation occurred at a rate (8.6 micrometer/min) that did not differ significantly from the rate (7.6 micrometer/m) in unirradiated cells ...	1981	7309791
metabolites of fusarium solani related to dihydrofusarubin.		1980	7251476
ecological distribution of fusarium solani and its opportunistic action related to mycotic keratitis in cali, colombia.	corneal ulcera in patients treated at the university hospital cali, colombia have been attributed to the fungus fusarium solani, which was isolated from patients' eyes by deep scraping. the fungus, which was characterized by culture and morphology, was found to grow well at 37 degrees c in sabouraud and potato dextrose agars and in liquid asparagine medium, in which it produced very few spores; at 40 degrees c, it survived for 3 weeks. different levels of pathogenicity were shown by the fungus w ...	1980	7217337
interference in the gas chromatographic determination of deoxynivalenol in cultures of fusarium solani on corn.		1982	7182412
fusarium solani keratitis.		1982	7169026
the immunosuppressive effects of trichothecenes and cyclochlorotine on the antibody responses in guinea pigs.	immunosuppressive effects of trichothecenes of fusarium solani and fusarium nivale, t-2 toxin and fusarenon-x, and also of a mycotoxin of penicillium islandicum, cyclochlorotine, were studied by measuring the anti-2,4-dinitrophenyl (dnp) antibody responses in guinea pigs immunized with dnp-bovine serum albumin. among these mycotoxins, t-2 toxin alone suppressed strongly the anti-dnp antibody responses at a certain sublethal dose. with other mycotoxins, no effect was observed at any sublethal dos ...	1982	7120038
[perforatory keratoplasty for keratitis due to fusarium solani].		1981	7024056
fusarium solani endophthalmitis.		1980	6966137
studies on the etiology and symptomatology of root and storage rot disease of cocoyam in nigeria.	the losses caused by root and storage rot of cocoyam in nigeria are estimated as 40 to 45%. field symptoms of the disease include inhibited growth, leaf chlorosis followed by necrosis and shrivelling of affected parts, and finally premature death of the aerial portions of the plant. a large proportion of the roots are destroyed. poor production of cormels and reduced corm size are other field symptoms of the disease, differing according to the type of causal agent. botryodiplodia theobromae, fus ...	1982	6891209
ovicidal fungi in soils of cuba.	thirty-six strains of ovicidal fungi were isolated from 122 investigated soil samples collected in cuba. the isolated strains belong to 8 genera, 2 strains consist of sterile mycelium only. most of the strains belong to the genus fusarium (4 species, 1 undetermined strain), the remaining ones to mortierella (3 species and 6 undetermined strains), humicola (2 species), paecilomyces (2 species) and penicillium (2 species). the genera verticillium, gliocladium and cunninghamella were represented by ...	1982	6890012
septic arthritis due to fusarium solani.	a case of fusarium arthritis is reported. fusarium, a mold ubiquitous in soil and on plants commonly causes keratomycosis and infects burns. recent reports demonstrate that fusarium may produce serious visceral infection in compromised hosts. disseminated infection has been universally fatal. in contrast, our case and several others with localized visceral infection were cured by intravenous amphotericin b and drainage. although fusarium grows readily on mycologic media, blood and other cultures ...	1983	6842474
mechanism of action of cutinase: chemical modification of the catalytic triad characteristic for serine hydrolases.	cutinase from fusarium solani f. sp. pisi was inhibited by diisopropyl fluorophosphate and phenylboronic acid, indicating the involvement of an active serine residue in enzyme catalysis. quantitation of the number of phosphorylated serines showed that modification of one residue resulted in complete loss of enzyme activity. one essential histidine residue was modified with diethyl pyrocarbonate. this residue was buried in native cutinase and became accessible to chemical modification only after ...	1982	6809046
formation of trichothecenes by fusarium solani var. coeruleum and fusarium sambucinum in potatoes.	fusarium solani var. coeruleum can form deoxynivalenol in potato tubers and in liquid medium, although concentrations observed in the rot were highly variable; acetyldeoxynivalenol and ht-2 toxin were detected in 1 to 3 tubers only (of 57). trichothecenes were also detected in a very few (3 of 20) cultures of fusarium sambucinum in potato tubers.	1984	6742832
studies on the antigenic composition of aqueous cotton dust extracts.	an immune response to cotton dust components has been proposed as a possible mechanism of byssinosis. to evaluate this possibility, specific precipitating antibodies against aqueous cotton dust extracts (acde) were raised in rabbits, and a reference pattern for crossed immunoelectrophoresis (cie) was established using this extract. a significant portion of the immune response was directed against alternaria tenuis, aspergillus niger, and fusarium solani, all documented contaminants of cotton dus ...	1983	6642705
relative efficacy of miconazole against rhizoctonia sp., paecilomyces lilacinus and fusarium solani.		1983	6618536
anterior segment resection because of fusarium solani keratitis and endophthalmitis.		1984	6610405
degradation of humic acids, extracted from manure and soil by some streptomycetes and fungi.	humate-decomposing microorganisms were isolated from alluvial soil, farmyard manure and peat by direct plating and enrichment techniques. peat was found to harbour more of these organisms than soil and manure. out of 21 actinomycetes and 6 fungi 9 and 3 isolates, respectively, were found to utilize manure-humate as a sole source of carbon and to a less extent as a source of both carbon and nitrogen in liquid medium. these efficient cultures were identified as streptomyces spec. (a 11 and a 12). ...	1980	6448526
recurrent fusarium keratomycosis: a light and electron microscopic study.	corneal buttons infected with fusarium solani, were removed during penetrating keratoplasty, and studied by light and electron microscopy. histologically, it appeared that the hyphae penetrated descement 's membrane and the posterior stroma from the anterior chamber. their cell wall, plasmalemma, and cytoplasm were preserved indicating that the fungi might be still active despite antifungal therapy, which may not be effective once the fungus penetrates descemet's membrane. the other interesting ...	1984	6372614
[antifungal and antimicrobial activity of beta-ionone and vitamin a derivatives].	the antifungal and antimicrobic activity of some derivatives of beta-ionon and vitamin a was studied. these compounds (citral, pseudo-ionon. beta-ionon aldehyde c14, ketone c18 and its derivatives--4,18-diketone, alcohol c18, semicarbazide ketone c18), as well as vitamin a and its derivatives--retinal, acetate, retinoic acid--differ in composition, structure and substituents of c-atoms in beta-ionon ring and in polyenoic chain. fusarium solani, botrytis cenerea and verticillum dahliae ii, race 4 ...	1983	6364128
comparative effect of amphotericin b on rhizoctonia sp., paecilomyces lilacinus & fusarium solani in vitro & in experimental keratitis.		1981	6268532
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.	we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...	1983	6195179
susceptibility and resistance of several fungi to microbial lysis.	potgieter, h. j. (cornell university, ithaca, n.y.), and m. alexander. susceptibility and resistance of several fungi to microbial lysis. j. bacteriol. 91:1526-1532. 1966.-strains of streptomyces, nocardia, and pseudomonas capable of lysing hyphae of fusarium solani or neurospora crassa were obtained by selective culture, but attempts to isolate an organism lysing rhizoctonia solani failed. when provided with f. solani or n. crassa as carbon sources, the actinomycetes tested produced beta-(1 --> ...	1966	5929777
hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes of fusarium solani f. phaseoli.		1966	5901783
effects of tannins on spore germination and growth of fusarium solani f. phaseoli and verticillium albo-atrum.		1967	5625600
ribosomal competence and spore germination in fusarium solani.	extracts prepared from macroconidia of fusarium solani f. sp. phaseoli are capable, under defined conditions, of incorporating phenylalanine into polypeptide with exogenous polyuridylic acid as messenger. extracts from ungerminated and germinated spores have approximately the same activity. with endogenous template, leucine incorporation occurs, but in this reaction extracts from germinated spores have about 10 times more activity than do those from ungerminated spores. it is suggested that the ...	1971	5573727
[plants affecting the phytopathogenic fungi. iv. fusarium solani f. pisi (jones) snyd. et hans].		1970	5535932
exogenous carbon and nitrogen requirements for chlamydospore germination by fusarium solani: dependence on spore density.		1970	5521394
chlamydospore development in the absence of protein synthesis in fusarium solani.		1970	5485812
carbon and nitrogen requirements for macroconidial germination of fusarium solani: dependence on conidial density.		1970	5484061
herbicide transformation. ii. studies with an acylamidase of fusarium solani.	replacement cultures liberated 3,4-dichloroaniline (dca) from 3,4-dichloropropionanilide (propanil). the kinetics of the conversion suggest a requirement for de novo enzyme synthesis, but the system was not influenced by chloramphenicol or puromycin. enzyme activity was detected when acetanilide (k(m) = 0.195 mm) was used to replace propanil as substrate. fungal acylamidase (e.c. 3.5.1., an aryl acylamine amidohydrolase) was concentrated by salt precipitation and characterized. the fusarium sola ...	1970	5437306
herbicide transformation. i. studies with whole cells of fusarium solani.	a strain of fusarium solani isolated from soil by enrichment techniques used propanil (3', 4'-dichloropropionanilide) as a sole source of organic carbon and energy for growth in pure culture. the primary product of the transformation of propanil by f. solani was isolated and identified as 3,4-dichloroaniline (dca). this compound accumulated in the medium to a level (80 mug/ml) which stopped further herbicide utilization. herbicide utilization by f. solani was influenced by various environmental ...	1970	5437305
induction of chlamydospore formation in fusarium solani by soil bacteria.		1970	5436564
the cellulase of fusarium solani. resolution of the enzyme complex.	1. culture filtrates from fusarium solani were fractionated by ion-exchange chromatography on deae-sephadex, followed by gel chromatography on sephadex g-100, into a c(1) component, a c(x) component (cm-cellulase) and a beta-glucosidase (cellobiase) component. 2. the individual components showed little capacity for the solubilization of cotton fibre (cellulase activity), but when recombined in their original proportions 81% of the original cellulase activity was recovered. 3. the c(1) components ...	1969	5389535
proteins and catalase isoenzymes from fusarium solani and their taxonomic significance.		1967	5340501
the cellulase of fusarium solani. purification and specificity of the -(1-4)-glucanase and the -d-glucosidase components.	1. cell-free culture filtrates of the fungus fusarium solani were examined for homogeneity with respect to beta-d-glucosidase and c(x) activities. 2. o-nitrophenyl beta-d-glucoside and cellobiose were both used as substrates for beta-d-glucosidase activity. 3. no evidence for the non-identity of nitrophenyl beta-d-glucosidase and cellobiase activities could be found, either by heat treatment, gel filtration on sephadex g-100 or by isoelectric focusing. 4. the beta-d-glucosidase component was als ...	1971	5119766
reduction of the 20-carbonyl group of c-21 steroids by spores of fusarium solani and other microorganisms. i. side-chain degradation, epoxide cleavage, and substrate specificity.	the spores of fusarium solani reduced the c(2)-carbonyl group, 1-dehydrogenated ring "a" and cleaved the side chain of 16alpha, 17alpha-oxidopregn-4-ene-3, 20-dione (16alpha, 17alpha-oxidoprogesterone)(i) to give the following products: 20alpha-hydroxy-16alpha, 17alpha-oxidopregn-4-en-3-one(ii); 20alpha-hydroxy-16alpha, 17alpha-oxidopregna-1, 4-dien-3-one(iii); 16alpha-hydroxy-17a-oxa-androsta-1, 4-diene-3, 17-dione (16alpha-hydroxy-1-dehydrotestololactone)(iv); and 16alpha, 17beta-dihydroxy-and ...	1972	5021973
chlamydospore induction in pure culture in fusarium solani.		1971	4935359
corneal and intra-ocular infection due to fusarium solani.		1970	4925294
depolymerization of a hydroxy fatty acid biopolymer, cutin, by an extracellular enzyme from fusarium solani f. pisi: isolation and some properties of the enzyme.		1973	4784475
effect of aureofungin on seedling mortality and growth of two varieties of sesame with seed-borne infection of fusarium solani.		1973	4763647
steroid 1-dehydrogenation and side-chain degradation enzymes in the life cycle of fusarium solani.		1973	4756113
modification of the exogenous carbon and nitrogen requirements for chlamydospore germination of fusarium solani by contact with soil.		1973	4752344
distribution of steroid 1-dehydrogenation and side-chain degradation enzymes in the spores of fusarium solani: causes of metabolic lag and carbohydrate independence.	the spores of a strain of fusarium solani 1-dehydrogenate ring a and cleave the 17beta-acetyl side chain of 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-hydroxyprogesterone) to give 17alpha-hydroxypregna-1,4-diene-3,20-dione (the 1-dehydro analogue) and little androsta-1,4-diene-3,4-diene-3,17-dione (androstadienedione). a 4-h lag period is observed in the course of metabolism, and there are no requirements for external additives. exoenzymes or surface enzymes bound to the cell outside the pla ...	1973	4699223
observations on strains of fusarium solani, f. oxysporum and candida parapsilosis from ulcerated legs.		1972	4553714
toxicological approaches to the metabolites of fusaria. iv. microbial survey on "bean-hulls poisoning of horses" with the isolation of toxic trichothecenes, neosolaniol and t-2 toxin of fusarium solani m-1-1.		1972	4538152
fusarium solani keratitis treated with natamycin (pimaricin): eighteen consecutive cases.		1972	4537911
fusarium solani infection during treatment for acute leukemia.		1973	4518930
solaniol, a toxic metabolite of fusarium solani.	fusarium solani m-1-1 isolated from moldy bean hulls produces t-2 toxin, diacetoxyscirpenol, and a new toxic trichothecene, solaniol, in czapek-dox-peptone medium.	1971	4331775
calcofluor and ink-potassium hydroxide preparations for identifying fungi.	calcofluor and ink-potassium hydroxide preparations identified fusarium solani, aspergillus fumigatus, and candida albicans, the three most common ocular fungal pathogens, in scrapings, biopsy specimens, and tissue sections of corneal mycotic infections in rabbits. these stains also identified fungal organisms in specimens from four human patients with keratomycoses. neither procedure requires more than a few minutes to perform or extensive training or experience to interpret. the specimen stain ...	1985	4061554
high field and 2d-nmr studies with the aporphine alkaloid glaucine.	the aporphine alkaloid glaucine (1) was examined by comparison of the high field (600 mhz) 1h-nmr spectra of 1 vs. racemic 6a,7,7-trideutereoglaucine (4,5), by computer-simulated 1h-nmr spectra at 600 mhz, by using decoupled proton spectra, and two-dimensional cosy and hetcor experiments with 1 at 500 and 360 mhz, respectively, and using high field (90 mhz) 13c-nmr of s-(+)-glaucine (1). emphasis was placed on the resolution of the chemical shifts and coupling constants for the h-4 alpha, h-4 be ...	1986	3783155
isolation of a fusarium solani mutant reduced in cutinase activity and virulence.	fusarium solani isolate t-8 produces an extracellular enzyme, cutinase, which catalyzes the degradation of cutin in the plant cuticle. cutinase activity can be measured by the hydrolysis of either the artifical substrate, p-nitrophenylbutyrate (pnb), or radioactive cutin containing [14c]palmitic acid. in the present study, the culture filtrate contained basal levels of cutinase when t-8 was grown on acetate as a sole source of carbon. after mutagenesis, a cutinase-defective mutant (pnb-1) was id ...	1986	3782031
the pathogenicities of cylindrocarpon tonkinense and fusarium solani in the rabbit cornea.	the pathogenicity of cylindrocarpon tonkinense in the cornea was evaluated and compared with that of fusarium solani in rabbits. f. solani was inoculated into the right eyes of 14 rabbits and c. tonkinense was into the left eyes of same rabbits. the corneal lesions of both eyes were examined carefully by slit lamp every day for three weeks and the severity of infections were compared each other. for histopathologic study, several eyes were enucleated periodically. c. tonkinense has a pathogenici ...	1986	3762655
fusarium solani: prevalence of skin reactivity and antigenic allergenic analysis.	the prevalence of fusarium solani reactivity in atopic individuals with symptoms of mold allergy was assessed with skin test and rast. in addition, f. solani preparations were evaluated for antigenic/allergenic activity. atopic individuals tested, 24.5% (n = 69), had positive skin reactions to a phosphate-buffered saline extract of f. solani, and these responses were statistically correlated with rast results. immunoelectrophoretic techniques demonstrated that this extract was antigenic in rabbi ...	1986	3711551
the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases.	two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ...	1986	3707549
fast method for two-dimensional electrophoresis of proteins from biological samples.	the method for two-dimensional gel electrophoresis of j. klose and m. feller [(1981) electrophoresis 2, 12-24] has been simplified by reducing the thickness of the gels from 3.5 to 1.1 mm for isoelectric focusing gels and from 3.5 to 0.84 mm for sodium dodecyl sulfate slab gels. thin gels need less reagents and smaller sample volumes. cooling of the thin gels during electrophoresis is more effective, which allows the use of higher electric power. therefore, less time is required for an electroph ...	1987	3674367
enzymatic hydrolysis of diethylpyrocarbonate, a commonly used histidine modifying agent, by esterases.	diethylpyrocarbonate, a reagent commonly used to modify active site histidines in enzymes, was found to be hydrolyzed by several esterases. two of these, cutinase, a typical serine esterase from the fungus fusarium solani pisi, and thioesterase b from the uropygial gland of the mallard duck anus platyrhynchus, hydrolyzed diethylpyrocarbonate so rapidly that histidine modification could not be detected except when the enzymic activity was inhibited by diisopropylfluorophosphate treatment or by th ...	1987	3595987
antibiotic activity of the pyrenocines.	pyrenocine a, a phytotoxin produced by pyrenochaeta terrestris (hansen) gorenz, walker and larson, possesses general antibiotic activity against plants, fungi, and bacteria. effective doses for 50% inhibition (ed50s) are 4 micrograms/ml for onion seedling elongation; 14, 20, 20, and 25 micrograms/ml for the germination of asexual spores of fusarium oxysporum f. sp. cepae, fusarium solani f. sp. pisi, mucor hiemalis, and rhizopus stolonifer, respectively. pyrenocine a also inhibits the linear myc ...	1987	3594310
direct examination vs culture of biopsy specimens for the diagnosis of keratomycosis.	in two patients with fungal keratitis, direct examination of corneal biopsy specimens showed positive fungal elements, but cultures of biopsy specimens failed to disclose fungal growth. we compared the value of direct examination and culture of biopsy specimens in the diagnosis of keratomycosis in rabbits with experimental fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. cultures disclosed seven specimens (70%) positive for candida and eight (80%) for fusa ...	1987	3578461
microbiological diagnosis of suppurative keratitis in bangladesh.	experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ...	1987	3555609
comparison of the pathogenicities of fusarium solani and candida albicans in the rabbit cornea.	the pathogenicity of f. solani was compared with that of candida albicans in experimental keratitis in rabbits. f. solani was inoculated into the right eye of each rabbit, and c. albicans into the left eye. the corneal lesions in both eyes were examined by slit lamp every day for 3 weeks and the severity of infections compared clinically and histopathologically. f. solani produced significantly more severe clinical infection in the cornea, compared to c. albicans. histopathologically, f. solani ...	1986	3537261
fungemia due to fusarium solani in an immunocompromised child.		1986	3523454
rapid visualization of three common fungi using fluorescein-conjugated lectins.	the feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. using a panel of fluorescein-conjugated lectins, candida albicans, aspergillus fumigatus, and fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed histopathologic specimens taken from experimental mycoses. additionally, aspergillus and fusarium were consistently differenti ...	1986	3514531
corneal biopsy in the diagnosis of keratomycosis.	in two patients, a 55-year-old man and a 49-year-old man, who had fungal keratitis initially undiagnosed by corneal scrapings the condition was successfully diagnosed by corneal biopsy. we compared corneal biopsy specimens and corneal scraping in the diagnosis of keratomycosis in rabbits with experimental bilateral fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. corneal scrapings disclosed three specimens (30%) positive for candida, five (50%) for fusariu ...	1986	3485380
disseminated hyalohyphomycosis in a leukemic patient.	a 69-year-old man with acute myeloblastic leukemia was admitted to the hospital for induction chemotherapy. a complication caused by a disseminated mycotic infection occurred while the neutropenic patient was maintained on a course of cytotoxic drugs, antibiotics, and corticosteroid therapy. the causal isolate was identified as fusarium solani on the basis of its colonial and microscopic morphology. this fungus developed in the patient's tissue in the form of hyaline, branched, septate hyphae. t ...	1986	3464232
granulomatous peritonitis in fish associated with fusarium solani.		1987	3438995
two linear plasmids in mitochondria of fusarium solani f. sp. cucurbitae.	two linear plasmid-like dnas designated pfsc1 (9.2 kbp) and pfsc2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus fusarium solani f. sp. cucurbitae race 1. the plasmids were maternally inherited and copurified with mitochondrial dna obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. the plasmids did not share extensive sequence similarity. no homology was detected between either plasmid and the nuclear or mitochondrial genome w ...	1988	3399553
acute bovine pulmonary emphysema caused by the fungus fusarium semitectum.	acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse.	1988	3388752
cutaneous infection caused by fusarium solani and fusarium oxysporum.		1987	3325538
pectate lyase from fusarium solani f. sp. pisi: purification, characterization, in vitro translation of the mrna, and involvement in pathogenicity.	since indirect experimental evidence suggested that penetration of fusarium solani f. sp. pisi into its host (pisum sativum) involved pectin-degrading enzymes (w. köller, c. r. allan, and p. e. kolattukudy (1982) physiol, plant pathol. 20, 47-60), direct tests were made for the production of such degradative enzymes by this pathogen. when the organism was grown on pectin, a pectate lyase (ec 4.2.2.2) was released into the media. this lyase was purified to apparent homogeneity from the culture fi ...	1987	3310898
disseminated cutaneous fusarium infection with vascular invasion in a leukemic patient.	a 61-year-old male with leukemia manifested multiple cutaneous nodules on his whole body surface, subcutaneous nodules on his arms and a tongue tumor. septate hyphae were observed microscopically in scrapings from the surfaces of the cutaneous lesions. fusarium solani and f. anthophilum were isolated from scrapings from the several skin lesions. histological examination revealed the presence of numerous septate hyphae in the lumina of vessels in the dermis. the fungal elements in the cutaneous t ...	1987	3302198
mycoflora of air-conditioners dust from riyadh, saudi arabia.	using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ...	1988	3236219
[endophthalmitis caused by the microscopic fungus fusarium solani].		1988	3197144
fungal keratitis in contact lens wearers.	in a retrospective review from 1972 through 1987 of patients with microbial keratitis, fungal infection occurred in four (4%) of 90 cosmetic or aphakic contact lens wearers and in four (27%) of 15 patients using a therapeutic soft contact lens. predisposing factors included improper lens care by the refractive lens wearers and a chronic epithelial defect with topical corticosteroid use among the therapeutic lens wearers. the responsible organisms in the refractive lens group were fusarium solani ...	1988	3195650
fusarium solani osteomyelitis with probable nosocomial spread.	a case of fusarium solani osteomyelitis is reported in a previously healthy adult male who received multiple injuries during an automobile accident. combined histologic and mycologic data proved it to be a case of hyalohyphomycosis resulting from fusarium. to the best of the authors' knowledge, this is the first reported case of osteomyelitis resulting from f. solani. a concurrent case of saprophytic wound colonization by the same organism is reported in another patient in the same ward who had ...	1988	3195503
[fractional and fatty acid composition of the lipids in the fungus fusarium solani and its oligoconidial mutants].		1988	3185313
invasive fusarium solani infections in patients with acute leukemia.	two cases of disseminated hyalohyphomycosis due to fusarium solani in patients with acute nonlymphocytic leukemia were studied. the clinical features in both patients included fever, fungemia, severe myalgias, disseminated ecthyma gangrenosum-like skin lesions, ocular symptoms, and a fatal outcome despite systemic administration of amphotericin b in the setting of profound, persistent granulocytopenia. in vitro studies showed the resistance of both f. solani isolates to amphotericin b, 5-fluoroc ...	1988	3164881
purification and characterization of cutinase from a fluorescent pseudomonas putida bacterial strain isolated from phyllosphere.	cutinase, an extracellular enzyme, was induced by cutin in a fluorescent pseudomonas putida strain that was found to be cohabiting with an apparently nitrogen-fixing corynebacterium. this enzyme was purified from the culture fluid by acetone precipitation followed by chromatography on deae-cellulose, qae-sephadex, sepharose 6b, and sephadex g-100. the purified enzyme showed a single band when subjected to polyacrylamide electrophoresis and the enzymatic activity coincided with the protein band. ...	1988	3130804
disseminated fusarium solani infection with cutaneous nodules in a bone marrow transplant patient.	fusarium is a ubiquitous fungus that commonly colonizes ulcerated, burned, or traumatized skin and may cause keratitis and onychomycosis in healthy hosts. serious disseminated infection due to fusarium has been reported with increasing frequency in immunocompromised patients. we describe a bone marrow transplant patient who developed fungal septicemia and disseminated skin nodules due to fusarium solani. fusarium should be recognized as a potential cause of deep fungal infection in immunocomprom ...	1988	3069758
diagnosis and successful treatment of fusariosis in the compromised host.	we present six cases of fusariosis caused by fusarium solani that were diagnosed over a three-year period in 166 adult patients undergoing chemotherapy for acute leukemia. necrotic skin lesions were evident in four patients, fungemia in three, and a deep cellulitis around a great toe nail at the time of a febrile illness in two. the mean minimal inhibitory concentration (mic) of amphotericin b was 3.3 micrograms/ml and of miconazole, 5.3 micrograms/ml; all isolates were resistant to 5-fluorocyto ...	1988	3053920
fusarium oxysporum keratitis.	we describe herein a patient with a rare occurrence of mycotic keratitis caused by fusarium oxysporum. the fungus destroyed the cornea, and a perforating corneal graft had to be performed. the drug of choice in such diagnosed cases is natamycin. a comparison between fusarium oxysporum and fusarium solani is discussed.	1989	2919034
antifungal susceptibility of 44 clinical isolates of fusarium species determined by using a broth microdilution method.	the mics and minimum fungicidal concentrations of amphotericin b, natamycin, miconazole, itraconazole, and flucytosine against 17 isolates of fusarium solani, 14 isolates of fusarium moniliforme, 10 isolates of fusarium oxysporum, and 3 isolates of fusarium semitectum were determined by a broth microdilution method. amphotericin b and natamycin were the most active agents tested and failed to show any inoculum size effect. in contrast, miconazole and itraconazole showed poor inhibitory and fungi ...	1989	2817867
fatal fusarium solani infections in baby sharks.	the occurrence of fatal fusariosis in baby bonnethead sharks (sphyrna tiburo) born at the national aquarium, baltimore, maryland, is reported. an atypical strain of fusarium solani was cultured from the tissues of two of the infected sharks following postmortem examination. histopathology revealed an apparent predilection of the fungus for hyaline cartilage. invasion of the cartilage resulted in hyphae with a distorted morphology. in slide culture the fungus displayed the unusual characteristic ...	1989	2746438
structure of the cutinase gene and detection of promoter activity in the 5'-flanking region by fungal transformation.	the cutinase gene from fusarium solani f. sp. pisi (nectria hematococa) was cloned and sequenced. sau3a fragments of genomic dna from the fungus were cloned in a lambda charon 35 vector. when restriction fragments generated from the inserts were screened with 5' and 3' probes from cutinase cdna, a 5.5-kilobase ssti fragment hybridized with both probes, suggesting the presence of the entire cutinase gene. a 2,818-base pair segment was sequenced, revealing a 690-nucleotide open reading frame that ...	1989	2703464
potted plants in hospitals as reservoirs of pathogenic fungi.	the soils of five potted plants cultivated within a hospital were investigated for the presence of fungal opportunistic pathogens of humans. a total of 16 potentially pathogenic species were isolated, including aspergillus fumigatus at up to 53.5 colony-forming units (cfu) per gram dry soil and scedosporium apiospermum (pseudallescheria boydii) at up to 97.0 cfu/g. other common species included phialophora verrucosa and fusarium solani. scedosporium inflatum, a recently described emerging pathog ...	1989	2671744
characterization of the termini of linear plasmids from nectria haematococca and their use in construction of an autonomously replicating transformation vector.	the mitochondria of isolate fs37 from nectria haematococca mating population i (fusarium solani f. sp. cucurbitae) contain two linear plasmids, pfsc1 and pfsc2, of 9.2 and 8.3 kbp, respectively. evidence for a protein blocking the 5' termini of these plasmids was obtained from exonuclease digestion experiments. a single protein band with an apparent mr of 80 k was labeled when the dna-protein complex of either plasmid was reacted with [125i] bolton-hunter reagent and then digested with dnase i. ...	1989	2598274
fusariosis in the shrimp penaeus semisulcatus cultured in israel.	the first case of a mycotic infection in shrimp in israel is reported. fusarium solani produced a large melanized lesion in a specimen of penaeus semisulcatus cultured at eilat, on the red sea. fungal hyphae elicited a strong hemocytic response in cuticular and connective tissues. in the underlying muscle, the inflammatory reaction appeared weaker, suggesting a gradual failure by the host to resist mycelial invasion. three cases of human keratomycosis by f. solani have been reported in recent ye ...	1989	2594048
molecular transformation of fusarium solani with an antibiotic resistance marker having no fungal dna homology.	a vector was constructed for transformation of the plant pathogenic fungus fusarium solani. the promoter 35sp, from cauliflower mosaic virus, was fused to the bacterial gene aph(3')ii, which confers resistance to the aminoglycoside antibiotic g418. two transformation procedures were developed: one using isolated fungal protoplasts, the other using germinated fungal spores. a transformation frequency of 3.3 g418-resistant colonies were obtained per microgram dna. of 14 colonies analyzed, 12 had v ...	1989	2550150
[search for actinomycetes--antagonists of fungi causing sugar beet root rot].	actinomycetes belonging to streptomyces were isolated from the rhizosphere of sugar beet grown on an infected area after cultivation for many years. 44.1 per cent of them proved to be antagonists of phytopathogenic test fungi. the majority of the antagonists were detected among the cinereus. the lowest number of the antagonists was detected among the azureus, the maximum number of the antagonists was observed in the middle and at the end of the sugar beet vegetation period. during various period ...	1989	2534467
fusarium solani: evidence for common antigenic/allergenic determinants with other fungi imperfecti.	aqueous extracts of fusarium solani and other members of the fungi imperfecti were evaluated for the presence of common antigenic/allergenic determinants using skin-prick testing, radio-allergo-sorbent test (rast) inhibition, and immunoelectrophoretic methods. prevalence of skin reactivity in forty-four atopic individuals, tested with commercially available fungal extracts, ranged from 27.3% for alternaria tenuis to 6.8% for penicillium notatum. no specific patterns of reactivity emerged from st ...	1987	2438068
the value of calcofluor white in the diagnosis of mycotic and acanthamoeba infections of the eye and ocular adnexa.	in search of an easy and quick method for the diagnosis of fungal and acanthamoeba infections of the eye, the authors have used calcofluor white (cfw), a fluorescent brightener with marked affinity for chitin and cellulose which are present in the cell walls of fungi and cysts of acanthamoeba. paraffin-embedded tissues containing mycotic organisms were stained with cfw and examined under a fluorescent microscope. several fungi such as candida sp, aspergillus sp, phycomycetes, fusarium solani, hi ...	1987	2436125