Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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prevalence of multiple drug resistance and screening of enterotoxin (stn) gene in salmonella enterica serovars from water sources in lagos, nigeria. | to determine the prevalence, antibiotic resistance and carriage of enterotoxin (stn) gene among strains of salmonella isolated from water sources in lagos. | 2011 | 21277605 |
[cloning and identification of ipaj gene in salmonella pullorum]. | cloning of ipaj gene from salmonella pullorum c79-13, and identification of expressed ipaj protein as an immunogen of the pathogen. | 2010 | 21268902 |
[srna (srab) regulate the resistant ability of salmonella enterica serovar enteritidis to egg albumen]. | salmonella species are important food-borne pathogens of human and animal. s. enterica serovar enteritidis is the only serovar that routinely causes human infection through intact egg, the molecular basis of its ability to survive in egg is poorly understood. the importance of post-transcriptional regulation by small non-coding rnas (srna) has recently been recognized. the srnas play diverse physiological roles in stress responses, regulation of metabolism, control of bacterial envelope composit ... | 2010 | 21268901 |
minimal antimicrobial peptidic sequence from hemoglobin alpha-chain: kyr. | hemoglobin is an animal protein described as a source of biologically active peptides. peptic digestion of bovine hemoglobin alpha-chain allowed obtaining peptide fractions with antimicrobial activity. these peptides were purified by reverse-phase high-performance liquid chromatography (hplc) and characterized by mass spectrometry. the minimal inhibitory concentration and mode of action of these peptides were studied against five bacterial strains including escherichia coli and salmonella enteri ... | 2011 | 21262306 |
erythrophore cell response to food-associated pathogenic bacteria: implications for detection. | cell-based biosensors have been proposed for use as function-based detectors of toxic agents. we report the use of betta splendens chromatophore cells, specifically erythrophore cells, for detection of food-associated pathogenic bacteria. evaluation of erythrophore cell response, using bacillus spp., has revealed that this response can distinguish pathogenic bacillus cereus from a non-pathogenic b. cereus δplcr deletion mutant and a non-pathogenic bacillus subtilis. erythrophore cells were expos ... | 2008 | 21261862 |
gene expression profiles induced by salmonella infection in resistant and susceptible mice. | mouse models have been extensively used to investigate the mechanisms of salmonellosis. however, the role of the hosts' local intestinal responses during early stages of infection remain unclear. in this study, transcript array analysis was employed to investigate regulation of gene expression in the murine intestine following oral challenge with salmonella enterica serovar enteritidis. salmonella resistant c3h/hen mice elicited only weak transcription responses in the ileum even in the presence ... | 2011 | 21256241 |
salmonella sp. in game (sus scrofa and oryctolagus cuniculus). | the role of wildlife in the epidemiology of salmonella sp.-induced diseases is a matter of increasing concern to public health. however, to date, reports on the occurrence of salmonella sp. in game hunted for human consumption are very limited. the current study was designed to evaluate the prevalence of salmonella sp. in fecal samples of wild boars and wild rabbits hunted in northern portugal. the results show that 22% of the (17/77) wild boar and 48% (38/80) of the wild rabbit presented salmon ... | 2011 | 21254910 |
application of phage typing and pulsed-field gel electrophoresis to analyse salmonella enterica isolates from a suspected outbreak in lagos, nigeria. | inadequate potable water supply and poor sanitation predispose to food- and water-borne diseases associated with salmonella enterica serovars in developing countries. in this study the possible source of an unprecedented upsurge of salmonella-associated community gastroenteritis was traced using both phage-typing and pulsed-field gel electrophoresis (pfge). | 2010 | 21252464 |
antimicrobial resistance in salmonella enterica serovar enteritidis in morocco. | salmonella enterica is recognised worldwide as one of the major agents of human gastrointestinal infections. the aim of the present work is to ascertain the antimicrobial susceptibilities of 150 salmonella enterica serovar enteritidis isolates from humans in morocco during the period from 2000 to 2008. | 2010 | 21252460 |
cyclic diguanylate signaling proteins control intracellular growth of legionella pneumophila. | proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. these processes include motility, biofilm formation, cell division, differentiation, and virulence. the role of cyclic diguanylate signaling in the lifestyle of legionella pneumophila, the causative agent of legionnaires' disease, has not previously been examined. the l. pneumophila genome encodes 22 predicted proteins containing domains related to cycli ... | 2011 | 21249170 |
salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. | bacterial ghosts (bgs) are empty bacterial envelopes generated by expulsion of the bacterial genome and cytoplasmic contents from bacterial cells, and the process is mediated by lysis protein e encoded on bacteriophage phix174. bgs represent a new approach in vaccine development and have been applied to a variety of gram-negative bacterial vaccine candidates. in this study, a bg vaccine generated from salmonella enteritidis (s. enteritidis) strain dh091 was prepared using the highly efficient pl ... | 2010 | 21247655 |
adjuvant effect of a probiotic fermented milk in the protection against salmonella enteritidis serovar typhimurium infection: mechanisms involved. | probiotics may offer protection against salmonella enteritidis serovar typhimurium infection via different mechanisms. the aim of this study is to investigate, using mouse models, the effect of the administration of fermented milk containing the probiotic bacteria l. casei dn-114 001 in the protection against salmonella enteritidis serovar typhimurium when this product is administered continuously before and after infection or only post-infection. the adjuvant effect of this probiotic fermented ... | 2010 | 21244773 |
the synthesis and characterization of poly(γ-glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity. | magnetite nanoparticles (mnps) modified with sodium and calcium salts of poly(γ-glutamic acid) (napga and capga) were synthesized by the coprecipitation method, followed by characterization and evaluation of their antibacterial and cytotoxic effects. superparamagnetic mnps are particularly attractive for magnetic driving as well as bacterial biofilm and cell targeting in in vivo applications. characterization of synthesized mnps by the fourier transform infrared spectra and magnetization curves ... | 2011 | 21233545 |
effects of mono- and disaccharides on the antimicrobial activity of bovine lactoperoxidase system. | the effects of mono- and disaccharides on the antimicrobial activity of the lactoperoxidase (lpo) system against salmonella enteritidis were investigated. the results clearly reveal that most of the sugars inhibit the antimicrobial activity of the lpo system. the inhibitory potency varies depending on the structure of sugar. l-fructose and d-allose were strongly inhibitive to the action of the lpo system, while sucrose was the weakest inhibitor. the decreased antimicrobial activity is due to the ... | 2011 | 21219776 |
inhibitory activity of cheese whey fermented with kefir grains. | we investigated the chemical and microbiological compositions of three types of whey to be used for kefir fermentation as well as the inhibitory capacity of their subsequent fermentation products against 100 salmonella sp. and 100 escherichia coli pathogenic isolates. all the wheys after fermentation with 10% (wt/vol) kefir grains showed inhibition against all 200 isolates. the content of lactic acid bacteria in fermented whey ranged from 1.04 × 10(7) to 1.17 × 10(7) cfu/ml and the level of yeas ... | 2011 | 21219767 |
effect of satureja thymbra essential oil on growth-no growth interfaces of listeria monocytogenes scott a and salmonella enteritidis pt4, at various temperatures, ph, and water activities. | antimicrobial efficacy of satureja thymbra essential oil against listeria monocytogenes scott a and salmonella ser. enteritidis pt4 was evaluated in vitro by modeling the growth boundaries at various temperatures, ph, and nacl concentrations. growth-no growth (turbidity) data were modeled by logistic polynomial regression. the concordance indices and the hosmer- lemeshow statistics of both logistic models indicated a good fit to the observed data. salmonella enteritidis was more sensitive at inc ... | 2011 | 21219762 |
salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials. | contamination of food contact surfaces by microbes such as salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. in the present study, salmonella enterica enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were asses ... | 2011 | 21219760 |
role of incubation conditions and protein fraction on the antimicrobial activity of egg white against salmonella enteritidis and escherichia coli. | the mechanism of egg white antimicrobial activity involves specific molecules and environmental factors. however, it is difficult to compare the data from the literature because of the use of various bacterial strains and incubation conditions. the aim of our study was to determine the effect of temperature, ph, inoculum size, and egg white protein concentration on egg white antimicrobial activity and to investigate the putative interactions among these factors by conducting a complete factorial ... | 2011 | 21219759 |
thermal death time measurement using thin flexible sleeves: a new experimental approach for determining microbial destruction kinetics in fluids of arbitrary viscosity. | the thermal death time kinetics of salmonella enteritidis (se) was measured in buffer, egg yolk, and albumen using thin layer plastic sleeves. the sleeves allowed for the loading and sampling of liquids of high or unusual viscosity, as in the case of yolk and albumen, and accepted relatively large volumes (2 to 3 ml) of fluid. the sleeves maintained the volume of the fluid in a thin layer and could be easily handled for heat exposure. the thin layer maintained one-dimensional heat transfer and m ... | 2010 | 21219753 |
salmonella infections in food workers identified through routine public health surveillance in minnesota: impact on outbreak recognition. | the frequency of salmonella-infected food workers identified through routine surveillance from 1997 to 2004 in minnesota was determined in order to evaluate the impact of surveillance on the detection of outbreaks in restaurants and to quantify the duration of salmonella shedding in stool. of 4,976 culture-confirmed salmonella cases reported to the minnesota department of health, 110 (2.2%) were identified as food workers; this was less than one-half the number expected based on the incidence of ... | 2010 | 21219717 |
site-directed (is30-flja) transposon mutagenesis system to produce nonflagellated mutants of salmonella enteritidis. | site-directed integration/mutagenesis systems are used to carry out targeted transpositions on dna. the well-characterized is30-element and its transposase have numerous advantages that predestine it to be a good candidate for such applications. in order to generate nonflagellated mutants of salmonella enteritidis, a new site-directed mutagenesis system has been developed and applied. the system was constructed based on the assumption that the dna-binding flja component of the fusion transposase ... | 2011 | 21219416 |
preparation and pharmacokinetics of ceftiofur sodium liposomes in cows. | the objective of this study was to prepare ceftiofur sodium liposomes and assess their physical properties, stability, antibacterial effects, and pharmacokinetics. these liposomes appeared as a milky, light yellow suspension with encapsulation efficiency at 57.2 ± 1.17%, and there were no significant changes in all estimated indexes at 4 °c for 90 days. the minimum inhibitory concentrations of liposomes were all 1/4th that of ceftiofur sodium against streptococcus suis, staphylococcus aureus, es ... | 2011 | 21219342 |
modulation of chicken macrophage effector function by t(h)1/t(h)2 cytokines. | regulation of macrophage activity by t(h)1/2 cytokines is important to maintain the balance of immunity to provide adequate protective immunity while avoiding excessive inflammation. ifn-γ and il-4 are the hallmark t(h)1 and t(h)2 cytokines, respectively. in avian species, information concerning regulation of macrophage activity by t(h)1/2 cytokines is limited. here, we investigated the regulatory function of chicken t(h)1 cytokines ifn-γ, il-18 and t(h)2 cytokines il-4, il-10 on the hd11 macrop ... | 2011 | 21208811 |
structures of the sep22 dodecamer, a dps-like protein from salmonella enterica subsp. enterica serovar enteritidis. | the crystal structure of sep22, a dna-binding protein from starved cells from salmonella enterica subsp. enterica serovar enteritidis, has been determined in two forms: the native state at 1.25 å resolution and an iron-soaked form at 1.30 å resolution. the sep22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. along the threefold axes of the 23 symmetry, hydrophilic asp channels that consist of asp ... | 2010 | 21206015 |
serotypes, antibiotic resistance, and class 1 integrons in salmonella isolates from pediatric cases of enteritis in tehran, iran. | the present study was conducted to investigate serotype distribution, antimicrobial resistance patterns, carriage of class 1 integron, and clonality of salmonella strains isolated from patients aged 0-12 years in tehran, iran, during 2007-2008. a total of 139 salmonella isolates were studied. salmonella serotypes enteritidis, infantis, and typhimurium included 84.9% of isolates, enteritidis accounting for 41.7%. the most prevalent resistances were to doxycycline (64.7%), nalidixic acid (61.2%), ... | 2011 | 21204690 |
description and burden of travel-related cases caused by enteropathogens reported in a canadian community. | risk of infections by enteropathogens among individuals traveling outside their country of residence is considered important. such travel-related cases (trc) have been poorly estimated and described in canada. | 2010 | 21199137 |
assessment of 2 salmonella enterica serovar typhimurium-based vaccines against necrotic enteritis in reducing colonization of chickens by salmonella serovars of different serogroups. | this study assessed the protective efficacy of oral vaccination with 2 experimental attenuated salmonella typhimurium-vectored vaccines for necrotic enteritis in protecting chickens against intestinal colonization by common serovars of salmonella belonging to the 4 major serogroups affecting chickens. birds were vaccinated orally with 1 × 10⁸ colony-forming units (cfu) of 1 of the vaccine strains χ9241 and χ9352, which express a plasmid-encoded partial recombinant hypothetical protein gene (thp) ... | 2010 | 21197226 |
foreign travel and decreased ciprofloxacin susceptibility in salmonella enterica infections. | to determine antimicrobial drug resistance patterns, we characterized nontyphoidal salmonella enterica strains isolated in liverpool, uk, january 2003 through december 2009. decreased susceptibility to ciprofloxacin was found in 103 (20.9%) of 492 isolates. the lower susceptibility was associated with ciprofloxacin treatment failures and with particular serovars and phage types often acquired during foreign travel. | 2011 | 21192872 |
the impact of different housing systems on egg safety and quality. | a move from conventional cages to either an enriched cage or a noncage system may affect the safety or quality, or both, of the eggs laid by hens raised in this new environment. the safety of the eggs may be altered either microbiologically through contamination of internal contents with salmonella enterica serovar enteritidis (salmonella enteritidis) or other pathogens, or both, or chemically due to contamination of internal contents with dioxins, pesticides, or heavy metals. quality may be aff ... | 2011 | 21177467 |
cloning and sequencing of biofilm-associated protein (bapa) gene and its occurrence in different serotypes of salmonella. | salmonella spp. has the capability to form biofilm on various surfaces. biofilm-associated protein (bapa), a large surface protein has been shown to play a leading role in the development of biofilm in salmonella. objective of this study was to investigate the presence of bapa gene in different serotypes of salmonella spp. and to characterize dna fragment encoding bapa protein of salmonella enteritidis. | 2010 | 21175701 |
stress-induced survival strategies enable salmonella enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white: a hypothesis to explain a pandemic. | abstract: | 2010 | 21172004 |
effect of e-beam treatment on the safety and shelf life of mayonnaise potato salad. | the radioresistance of listeria monocytogenes, salmonella enterica serovar enteritidis, and s. enterica serovar typhimurium has been studied in a complex matrix like mayonnaise potato salad. d(10)-values of 0.56, 0.32-0.35, and 0.41-0.42 kgy were calculated for each organism, respectively. keeping in mind these values, the microbiological criteria, the characteristics of the microorganisms, and a shelf life of the products of 20 days stored at 4°c, an irradiation treatment of 1 kgy was calculate ... | 2010 | 21166582 |
[septic shock in a renal transplanted patient after consumption of uncooked pork meat.] | salmonellosis caused by salmonella enteritidis is an acute and in most cases zoonotic disease, but chronic human carriers are also known. mostly, affected persons recover without treatment, but severe complications occur occasionally. for the first time we report a case of probably food-borne invasive salmonella enteritidis infection with septic shock in a patient with tacrolimus treatment, 13 years after renal transplantation, probably acquired by uncooked ground pork meat. | 2010 | 21161641 |
effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan. | chitosan is a biopolymer with antimicrobial activity and film-forming properties. in this study, the effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. a chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against salmonella enterica serovar enteritidis (s. enteritidis). for this purpose, a contact plate method was developed and chitosans were applied at a concen ... | 2010 | 21146239 |
the incredible inedible egg. | 2010 | 21140977 | |
survival of salmonella enteritidis pt 30 on inoculated almonds after commercial fumigation with propylene oxide. | propylene oxide (ppo) is commonly used to reduce microbial populations in u.s. bulk raw almonds, but the process has not been validated for reduction of foodborne pathogens. the reduction of salmonella enteritidis phage type (pt) 30 inoculated onto almonds was evaluated after exposure to a standard commercial ppo treatment. almonds were inoculated with salmonella enteritidis pt 30 to approximately 8.0 log cfu/g after drying. inoculated almonds were placed in bags designed for gaseous sterilizati ... | 2005 | 21132968 |
the renal cyst infection caused by salmonella enteritidis in a patient with autosomal dominant polycystic kidney disease: how did this pathogen come into the renal cysts? | a 40-year-old japanese man was admitted to our hospital for investigation of fever and right flank pain starting after watery diarrhea. salmonella enteritidis was cultured from samples of stool and blood. although his diarrhea subsided soon, high fever persisted and flank pain got worse. magnetic resonance imaging (mri) revealed four areas of abnormal intensity in renal cysts and computed tomography showed the same findings. renal cysts infection was diagnosed. after ultrasound-guided aspiration ... | 2010 | 21127936 |
sers-based sandwich immunoassay using antibody coated magnetic nanoparticles for escherichia coli enumeration. | a method combining immunomagnetic separation (ims) and surface-enhanced raman scattering (sers) was developed to enumerate escherichia coli (e. coli). gold-coated magnetic spherical nanoparticles were prepared by immobilizing biotin-labeled anti-e. coli antibodies onto avidin-coated magnetic nanoparticles and used in the separation and concentration of the e. coli cells. raman labels have been constructed using rod shaped gold nanoparticles coated with 5,5-dithiobis-(2-nitrobenzoic acid) (dtnb) ... | 2010 | 21125089 |
inactivation of cronobacter sakazakii by ultrasonic waves under pressure in buffer and foods. | the objective of this research was to characterize the resistance of cronobacter sakazakii to ultrasonic waves under pressure (manosonication, ms). the d(ms) value (decimal reduction time value) of c. sakazakii in standard conditions (35°c, 117 μm, 200 kpa, citrate-phosphate buffer ph 7.0) was 0.41 min. this value was higher than that of yersinia enterocolitica (d(ms)=0.19 min) and lower than those of salmonella enterica serovar enteritidis (d(ms)=0.61 min), listeria monocytogenes (d(ms)=0.86 mi ... | 2010 | 21111503 |
heterogeneity of type iii secretion system (t3ss)-1-independent entry mechanisms used by salmonella enteritidis to invade different cell types. | salmonella causes a wide range of diseases from acute gastroenteritis to systemic typhoid fever, depending on the host. to invade non-phagocytic cells, salmonella has developed different mechanisms. the main invasion system requires a type iii secretion system (t3ss) known as t3ss-1, which promotes a trigger entry mechanism. however, other invasion factors have recently been described in salmonella, including rck and pagn, which were not expressed under our bacterial culture conditions. based on ... | 2010 | 21109565 |
effect of serenoa repens, lycopene, and selenium on proinflammatory phenotype activation: an in vitro and in vivo comparison study. | to investigate the antiinflammatory activity of serenoa repens (ser), ly, and) on proinflammatory phenotype in rat peritoneal macrophages (ms) stimulated with salmonella enteritidis lipopolysaccharide (lps) and in the prostate of rats with partial bladder outlet obstruction. ser, combined with other compounds, such as ly and se is used to relieve symptoms associated with benign prostatic hyperplasia (bph). inflammation plays a pivotal role in the pathogenesis of bph and represents a target for a ... | 2010 | 21109292 |
high prevalence of integron-mediated resistance in clinical isolates of salmonella enterica. | salmonella enterica has become progressively resistant to antimicrobial agents worldwide as a result of genes carried on different classes of integrons. the aim of the current study was to investigate the molecular diversity of these integrons and their association with antimicrobial resistance in clinical s. enterica isolates from tehran, iran. antimicrobial susceptibility testing was performed according to the clinical and laboratory standards institute. the presence of integrons was investiga ... | 2010 | 21099092 |
avian-specific tlrs and downstream effector responses to cpg-induction in chicken macrophages. | chickens possess toll-like receptor (tlr15), a pattern recognition receptor (prr) absent in mammals. we characterized the regulation and mechanism of cpg responsiveness via tlrs in chicken macrophage hd11 cells. tlr15 was significantly upregulated after induction with b- and c-type cpg oligonucleotides (odn), tripalmitoylated lipopeptide (pam3csk4), escherichia coli- and salmonella enteritidis-derived lipopolysaccharide (lps). in response to cpg-odn inhibitor, tlr15 and il1b were downregulated, ... | 2010 | 21095203 |
prevalence of antimicrobial resistance among salmonella isolates from chicken in china. | we evaluated the antimicrobial resistance of salmonella isolated in 2008 from a chicken hatchery, chicken farms, and chicken slaughterhouses in china. a total of 311 salmonella isolates were collected from the three sources, and two serogroups of salmonella were detected, of which 133 (42.8%) consisted of salmonella indiana and 178 (57.2%) of salmonella enteritidis. the lowest percentage of s. indiana isolates was found in the chicken hatchery (4.2%), followed by the chicken farms (54.9%) and th ... | 2010 | 21083518 |
enzymes of spz7 phage: isolation and properties. | bacteriophage enzyme preparations exolysin and endolysin were studied. exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated salmonella enteritidis cells. a new method for purification of these enzymes was developed, and their molecular masses were determined. the main catalytic properties of the studied enzymes (ph optimum and specificity to bacterial substrates) were found to be similar. both enzymes lyse escher ... | 2010 | 21077836 |
dna-based diagnostic tests for salmonella strains targeting hila, agfa, spvc and sef genes. | the goal of this study was to evaluate the suitability of the hila, agfa, spvc and sef genes amplification by pcr as a method for detection of salmonella strains. twenty nine isolates of salmonella spp. including 6 different serotypes were analyzed in this study. the bacteria were isolated between 2005 and 2007 and serotyped at the clinical hospital of infectious disease, cluj-napoca. ten non-salmonella strains were also tested by the same procedure. we used a direct pcr technique, dna extractio ... | 2010 | 21071133 |
transcriptional profiling of antimicrobial peptides avian β-defensins in the chicken ovary during sexual maturation and in response to salmonella enteritidis infection. | avian β-defensins (avβds) are antimicrobial peptides that play significant roles in the innate immune system in chickens. the aim of this study was to identify the types of avβds expressed in the chicken ovary, to investigate the effects of sexual maturation in the ovarian mrna abundance and to determine the changes in their expression levels as a result to salmonella enteritidis (se) infection. rna was extracted from the ovary of healthy prepubertal, sexually mature and aged birds, as well as f ... | 2010 | 21071048 |
β 1-4 mannobiose enhances salmonella-killing activity and activates innate immune responses in chicken macrophages. | salmonella spp. is one of the major causes of food-borne illness in humans, and salmonella enteritidis (se) infection in commercial poultry is a world-wide problem. here we have investigated the in vitro immune-modulating effects of β 1-4 mannobiose (mnb), which was previously found to prevent se infection in vivo in chickens, using chicken macrophage (mq-mcsu) cells. treatment of mq-ncsu cells with mnb dose-dependently increased both phagocytic activity and salmonella-killing activity of macrop ... | 2010 | 21067819 |
multiplication of salmonella enteritidis in egg yolks after inoculation outside, on, and inside vitelline membranes and storage at different temperatures. | prompt refrigeration to restrict bacterial growth is important for reducing eggborne transmission of salmonella enterica serovar enteritidis (se). the nutrient-rich yolk interior is a relatively infrequent location for initial se deposition in eggs, but migration across the vitelline membrane can result in rapid bacterial multiplication during storage at warm temperatures. the objective of the present study was to measure the multiplication of se in yolks after introduction at three different lo ... | 2010 | 21067679 |
fates of foodborne pathogens in raw hams manufactured rapidly using a new patented method. | to manufacture raw ham in an efficient manner, we recently developed a new system in which presliced pork loin was used, and the processing time was reduced to 5% of the conventional method. this study aimed to examine whether this raw ham could be as safe as ham produced by the conventional method. pork loin spiked with enterohemorrhagic escherichia coli serotype o157:h7, listeria monocytogenes serotype 1/2c, salmonella enterica serovar enteritidis, and staphylococcus aureus were processed usin ... | 2010 | 21067667 |
[genetic mechanisms of salmonella enteritidis biodiversity and clinical features of salmonellosis]. | to assess prevalence of fragments of escherichia coli pathogenicity islands in salmonella enteritidis strains as well as to study clinical signs of disease caused by these strains in adults. | 2010 | 21064226 |
[use of enterocin s760 for prevention and treatment of experimental salmonella infection in mice]. | to demonstrate treatment efficacy of bacteriocin s760 synthesized by enterococcus faecium 760 for septic salmonella infection in mice. | 2010 | 21061577 |
[antibacterial antibodies in human immunoglobulins and sera: past and present]. | to measure levels of several types of antibacterial antibodies in preparations of normal human immunoglobulin as well as in samples of donor sera obtained in 1965 and 2009. | 2010 | 21061576 |
a mathematical model of inactivation kinetics for a four-strain composite of salmonella enteritidis and oranienburg in commercial liquid egg yolk. | the goal of this study was to develop a general model of inactivation of salmonellae in commercial liquid egg yolk for temperatures ranging from 58°c to 66°c by studying the inactivation kinetics of salmonella in liquid egg yolk. heat-resistant salmonellae (three serovars of enteritidis [two of phage type 8 and one pt 13] and one oranienburg) were grown to stationary phase in tryptic soy broth and concentrated 10-fold by centrifugation. each inoculum was added to liquid egg yolk and mixed thorou ... | 2010 | 21056777 |
production of monoclonal antibody against salmonella h: g,m flagellar antigen and potential diagnostic application. | in this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from salmonella enteritidis in a yield of 3.9 mg/ml(-1) after being concentrated with polyethylene glycol (peg). a monoclonal antibody (mab), designated d7 clone, was generated from balb/c mice immunized with salmonella enteritidis flagella using the conventional hybridoma method. the d7 clone secreting mab was classified as igg2a isotype. elisa analyses of d7 mab to salmonella-specific f ... | 2010 | 21050043 |
multiple contaminations of chickens with campylobacter, escherichia coli and salmonella in yaounde (cameroon). | food-borne diseases associated with campylobacter, escherichia coli, and salmonella are mainly caused by the consumption of raw or undercooked poultry meat. the objective of this study was to evaluate the prevalence of campylobacter, escherichia coli, and salmonella in chickens. | 2010 | 21045373 |
evaluation of bacterial multiplication in cleaning cloths containing different quantities of organic matter. | to determine a proper length of time for cleaning cloth usage, the present work aimed to evaluate bacterial multiplication in artificially contaminated cleaning cloths containing different amounts of organic matter. | 2010 | 21045369 |
sources of salmonella contamination during broiler production in eastern spain. | prevention of salmonella contamination of poultry products requires detailed knowledge of the main sources associated with its presence in the production system. the aims of this study were to determine the main sources of salmonella contamination in broiler production during growing, to assess the risk factors for salmonella contamination at the end of the rearing period and to determine the main serovars involved in broiler production systems in eastern spain. a total of 65 different broiler h ... | 2010 | 21035883 |
antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. | the chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: citrus aurantium, c. limon, lavandula angustifolia, matricaria chamomilla, mentha piperita, m. spicata, ocimum basilicum, origanum vulgare, thymus vulgaris and salvia officinalis have been determined. the antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed ... | 2010 | 21030907 |
transmission of salmonella enteritidis by pulex irritans and ctenocephalus canis. | 1946 | 20994016 | |
adhesion of pathogenic bacteria to food contact surfaces: influence of ph of culture. | the adhesion of aeromonas hydrophila, escherichia coli o157:h7, salmonella enteritidis, and staphylococcus aureus to hydrophobic and hydrophilic surfaces in cultures with different phs (6, 7, and 8) was studied. the results indicated that the type of material had no effect on the attachment capacity of microorganisms, while environmental ph influenced the adhesion of a. hydrophila, e. coli, and s. aureus to both solid substrates. the attachment of s. enteritidis (p > .05) was not affected by the ... | 2010 | 20981289 |
simultaneous analysis of multiple enzymes increases accuracy of pulsed-field gel electrophoresis in assigning genetic relationships among homogeneous salmonella strains. | due to a highly homogeneous genetic composition, the subtyping of salmonella enterica serovar enteritidis strains to an epidemiologically relevant level remains intangible for pulsed-field gel electrophoresis (pfge). we reported previously on a highly discriminatory pfge-based subtyping scheme for s. enterica serovar enteritidis that relies on a single combined cluster analysis of multiple restriction enzymes. however, the ability of a subtyping method to correctly infer genetic relatedness amon ... | 2010 | 20980570 |
transcriptional profiling of toll-like receptors in chicken embryos and in the ovary during sexual maturation and in response to salmonella enteritidis infection. | one of the key members of the innate immune system in many vertebrate species is the family of toll-like receptors (tlrs). these molecules, which initiate the innate immune response and mount an anti-microbial response in both vertebrates and invertebrates, have recently been identified in the chicken genome. the recent findings of chicken tlrs (ctlrs) expression in ovarian follicles during follicular growth and in response to lipopolysaccharide (lps) infection are very important for reproductiv ... | 2010 | 20970931 |
damage to the intestinal epithelial barrier by antibiotic pretreatment of salmonella-infected rats is lessened by dietary calcium or tannic acid. | perturbation of the intestinal microbiota by antibiotics predisposes the host to food-borne pathogens like salmonella. the effects of antibiotic treatment on intestinal permeability during infection and the efficacy of dietary components to improve resistance to infection have not been studied. therefore, we investigated the effect of clindamycin on intestinal barrier function in salmonella-infected rats. we also studied the ability of dietary calcium and tannic acid to protect against infection ... | 2010 | 20962149 |
puo-sevr1 is an emergent virulence-resistance complex plasmid of salmonella enterica serovar enteritidis. | 2010 | 20961912 | |
evaluation of the taxonomic utility of six-enzyme pulsed-field gel electrophoresis in reconstructing salmonella subspecies phylogeny. | pulsed-field gel electrophoresis (pfge) remains an important tool in the molecular epidemiological evaluation of strains emerging from disease outbreak clusters. recent studies of escherichia coli o157:h7 and salmonella enteritidis have noted marked improvements in the discriminatory power of pfge when combining band profiles from up to six restriction enzyme datasets into a single concatenated analysis. this approach has provided more accurate assignments of genetic relationships among closely ... | 2010 | 20959148 |
cross-reactivity of anti-salmonella egg-yolk antibodies to salmonella serovars. | the cross-reactivity of egg yolk antibodies specific to antigens of salmonella enteritidis and salmonella typhimurium to killed bacterial cells of common salmonella serovars were tested using an indirect enzyme linked immunosorbent assay (elisa). egg yolk antibodies were produced against purified fimbriae, flagella and lipopolysaccharide (lps) of s. enteritidis strain atcc13076 and flagella, lps and outer membrane proteins (omp) of s. typhimurium strain atcc13311. for immunological specificity o ... | 2010 | 20954046 |
development and evaluation of candidate recombinant salmonella-vectored salmonella vaccines. | attenuated salmonella enteriditis (δse) recombinant vaccine vectors incorporating a salmonella flagellar filament protein (flic) subunit, a putative cell-mediated epitope, for expression of the lamb gene (encoding a maltose outer membrane porin), with or without co-expression of a putative immune-enhancing cd154 oligopeptide, were developed and compared with wild-type salmonella enteriditis (experiments 1 and 2) or the attenuated δse empty vector (experiment 3) as initial vaccine candidates agai ... | 2010 | 20952699 |
efficacy of high-intensity pulsed light for the microbiological decontamination of chicken, associated packaging, and contact surfaces. | the efficacy of high-intensity light pulse (hilp) technology (3 hz, maximum of 505 j/pulse, and a pulse duration of 360 μs) for the decontamination of raw chicken and associated packaging and surface materials was investigated. its ability to reduce microbial counts on raw chicken through plastic films was also examined. complete inactivation of campylobacter spp., escherichia coli, and salmonella enteritidis in liquid was achieved after 30 sec hilp treatment. reductions of 3.56, 4.69, and 4.60 ... | 2010 | 20932088 |
differentiation of salmonella enteritidis isolates by fluorescent amplified fragment length polymorphism. | salmonella enteritidis is responsible for human gastroenteritis outbreaks worldwide, and the molecular characterization of isolates is an important tool for epidemiological studies. fluorescent amplified fragment length polymorphism (faflp) analysis was performed on 31 salmonella enteritidis strains from south brazil isolated from human, foods, swine, broiler carcasses, and other poultry-related samples to subtype isolates in comparison to pulsed-field gel electrophoresis (pfge) analysis. five s ... | 2010 | 20932084 |
cardiovascular complications of salmonella enteritidis infection. | salmonella has the ability to adhere to damaged endothelium, predisposing individuals to complications rarely seen with other gram-negative organisms. potential complications include endocarditis, infected atheroma or aneurysms, myocarditis and pericarditis. the present report describes two cases of salmonella enteritidis-associated cardiovascular disease. patient 1 is a young adult who presented with myopericarditis complicated by recurrent cardiac arrests following return from a tropical clima ... | 2010 | 20931102 |
sodium dodecyl sulfate-agarose gel electrophoresis for the detection and isolation of amyloid curli fibers. | curli are amyloid-like fibers on the surface of some strains of escherichia coli and salmonella enteritidis. we tested the use of horizontal sodium dodecyl sulfate (sds)-agarose gel electrophoresis to detect, isolate, and quantitate curli. cell extracts fractionated in sds-agarose gels and stained with coomassie blue exhibited a soluble fraction that entered the gel and an insoluble fraction that remained in the well. much more insoluble material was observed with curli-proficient strains than w ... | 2010 | 20920455 |
proteomic analysis of salmonella enterica serovar enteritidis following propionate adaptation. | salmonella enteritidis is a highly prevalent and persistent foodborne pathogen and is therefore a leading cause of nontyphoidal gastrointestinal disease worldwide. a variety of stresses are endured throughout its infection cycle, including high concentrations of propionate (pa) within food processing systems and within the gut of infected hosts. prolonged pa exposure experienced in such milieus may have a drastic effect on the proteome of salmonella enteritidis subjected to this stress. | 2010 | 20920181 |
evaluation of salmonella enterica serovar enteritidis pathogenicity island-1 proteins as vaccine candidates against s. enteritidis challenge in chickens. | salmonella enterica serovar enteritidis (s. enteritidis) is a major cause of gastrointestinal disease in humans worldwide, which mainly results from the consumption of contaminated poultry meat and eggs. vaccination of chickens is an important strategy to lower the prevalence of salmonella in poultry flocks. the s. enteritidis type 3 secretion system (t3ss) encoded on salmonella pathogenicity island-1 (spi-1) is an important virulence factor that plays a role in invasion and systemic spread in c ... | 2010 | 20888713 |
immune response following vaccination against salmonella enteritidis using 2 commercial bacterins in laying hens. | the humoral and cell-mediated immune (cmi) response to 2 commercial killed salmonella enteritidis (se) vaccines (layermune and mbl se4c) was evaluated in laying hens. layers were distributed in 2 experimental groups. the first received a single immunization at 16 wk of age, while the second experimental group was immunized at 12 wk of age and again at 18 wk of age. serum immunoglobulin (ig)g antibodies were measured using a commercial se elisa kit and showed persistent levels from 3 to 32 and 34 ... | 2010 | 20885842 |
salmonella enteritidis infection of the musculoskeletal system - case report. | this article presents diagnostic and therapeutic problems in 2 patients with blood-borne infection of the coxofemoral joint and the humeral diaphysis caused by salmonella enteritidis (s. enteritidis). these patients accounted for 4.6% of cases of blood-borne infections treated between 2002 and 2009 and 1.1% of all musculoskeletal infections treated at the department of orthopaedics and traumatology of n. copernicus regional specialized hospital in łódź. the inflammations developed nearly a year ... | 2010 | 20876930 |
efficacy of various dietary calcium salts to improve intestinal resistance to salmonella infection in rats. | previous animal and human studies have shown protective effects of ca on the resistance to enteropathogenic infections. most interventions were performed with calcium phosphate and little is known about the protective effect of other dietary sources of ca. therefore, we investigated the efficacy of several ca salts to enhance intestinal resistance to salmonella enteritidis infection. rats (n 7-8 per group) were fed a high-fat, western human-style, purified diet with a low ca content (20 mmol cal ... | 2010 | 20875217 |
the influence of the housing system on salmonella infections in laying hens: a review. | from 2012 onwards, housing of laying hens in conventional battery cages will be forbidden in the european union and only enriched cages and non-cage housing systems such as aviaries, floor-raised, free-range and organic systems will be allowed. although this ban aims at improving the welfare of laying hens, it has also initiated the question whether there are any adverse consequences of this decision, especially with respect to the spread and/or persistence of zoonotic agents in a flock. a zoono ... | 2010 | 20875073 |
salmonella cases traced to egg producers: findings trigger recall of more than 500 million eggs. | 2010 | 20858872 | |
visualization of gold and platinum nanoparticles interacting with salmonella enteritidis and listeria monocytogenes. | rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. the objective of the present investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-au and nano-pt respectively), with salmonella enteritidis (gram-negative) and listeria monocytogenes (gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles. | 2010 | 20856838 |
salmonella antibiotic-mutant strains reduce fecal shedding and organ invasion in broiler chicks. | we investigated the exposure to antibiotics in the production of antibiotic-mutant strains of salmonella. ten isolates of poultry origin were assayed for antibiotic susceptibilities. one strain of salmonella enteritidis, one of salmonella heidelberg, and one of salmonella typhimurium were selected to induce antimicrobial resistance. each strain was exposed to high concentrations of streptomycin, rifampicin, and nalidixic acid, respectively. parent and antibiotic-mutant strains were assayed for a ... | 2010 | 20852104 |
occurrence of purulent arthritis broilers vertically infected with salmonella enterica serovar enteritidis in korea. | salmonella enterica serovar enteritidis (salmonella enteritidis) has been associated with morbidity and mortality in broiler chickens worldwide. the present study described purulent arthritis of broilers infected with salmonella enteritidis and investigated antibiograms and genetic characteristics of salmonella enteritidis isolates from epidemiologically related properties such as a hatchery and breeder farm in an attempt to elucidate the source of contamination. clinical disease and mortality w ... | 2010 | 20852102 |
salmonella prevalence in commercial raw shell eggs in japan: a survey. | summarywe examined 20 300 raw shell chicken eggs sold at retail stores in japan for salmonella outside and inside eggs. the eggs were purchased at 220 retail stores throughout japan between august 2007 and january 2008. of 2030 pooled egg samples (10 eggs/sample), salmonella was isolated from five shell samples (0·25%), but not from any of egg-content samples. the serovars of the isolates were salmonella enteritidis (2), s. derby, s. livingstone and s. cerro. the samples positive for salmonella ... | 2010 | 20843388 |
effect of temperature and time of storage on protein stability and anti-salmonella activity of egg white. | hen egg white contains numerous molecules of interest for human health, including antimicrobial proteins. little information is available concerning changes in the antimicrobial activity of egg white during storage; therefore, we analyzed the potential of egg white to inhibit growth of salmonella enterica serovar enteritidis following storage at 4, 20, or 37°c for 30 days prior to inoculation. egg white displayed higher anti-salmonella activity after a few days of storage at 20 and 37°c. the rat ... | 2010 | 20828465 |
molecular and virulence characteristics of multi-drug resistant salmonella enteritidis strains isolated from poultry. | forty-six salmonella enterica subspecies enterica serovar enteritidis (s. enteritidis) strains were isolated from chicken meat, faeces, and eggshells collected from hatcheries throughout korea. the strains were examined for the presence of antimicrobial resistance and virulence genes. all 46 isolates were resistant to at least one of 21 antibiotics used in this study, 30 (65.2%) were resistant to three or more antimicrobials, and a single remarkable isolate was resistant to 15 antimicrobials. th ... | 2010 | 20822940 |
pulsed uv light inactivation of salmonella enteritidis on eggshells and its effects on egg quality. | the majority of salmonella enteritidis outbreaks have been related to the consumption of raw or undercooked eggs or egg-containing foods. therefore, the u.s. department of agriculture mandates egg washing for all graded eggs by use of a detergent solution and sanitizer. these agencies and the egg industry have been investigating alternative decontamination techniques, which could better serve the public, minimize costs, and benefit both the public and the industry. pulsed uv light is an emerging ... | 2010 | 20819349 |
a multiplex nanoparticle-based bio-barcoded dna sensor for the simultaneous detection of multiple pathogens. | a highly amplified, nanoparticle-based, bio-barcoded electrochemical biosensor for the simultaneous multiple detection of the protective antigen a (paga) gene (accession number, m22589) of bacillus anthracis and the insertion element (iel) gene (accession number, z83734) of salmonella enteritidis is reported in this paper. the biosensor system is mainly composed of three nanoparticles: gold nanoparticles (aunps), magnetic nanoparticles (mnps), and nanoparticle tracers (nts, such as pbs and cds). ... | 2010 | 20810267 |
differential phenotypic diversity among epidemic-spanning salmonella enterica serovar enteritidis isolates from humans or animals. | nontyphoidal salmonellae are major causes of food-borne disease worldwide. in uruguay, salmonella enterica serovar enteritidis was the most commonly isolated serovar throughout the last decade, with a marked epidemic period between 1995 and 2004. in a previous study, we conducted comparative genomics of 29 epidemic-spanning s. enteritidis field isolates, and here we evaluated the pathogenic potential of the same set of isolates using several phenotypic assays. the sample included 15 isolates fro ... | 2010 | 20802078 |
induction of a homologous and heterologous invasion-inhibition effect after administration of salmonella strains to newly hatched chicks. | administration of live salmonella strains to day-old chicks provides protection against infection within hours by intestinal colonisation-inhibition. however, the extent to which the oral application of live salmonella wild-type or vaccine strains may induce an early invasion-inhibition effect is unknown. potentially protective pre-treatment strains of salmonella enteritidis and infantis were examined for their ability (i) to colonise the caeca, to invade the liver, to induce an influx of granul ... | 2010 | 20732468 |
[salmonellosis in poland in 2008]. | in 2008, 9 608 salmonellosis cases were reported, the incidence was 25.2 per 100 000 population. among them 9 478 cases were intestinal and 130 were extraintestinal. salmonellosis was cause of death in 6 cases. in total 201 outbreaks were reported involving 1 647 cases. the most common type of outbreaks were household outbreaks. the most frequently isolated serotype in outbreaks and single cases was s. enteritidis. like in previous years the highest incidence was in children aged 2 (307 per 100 ... | 2010 | 20731226 |
video-assisted thoracoscopic surgery (vats) drainage of salmonella enteritidis empyema and needle-localization for retrieval of a dropped gallstone. | spillage of gallstones into the peritoneal cavity (dropped gallstones) is more common in laparoscopic compared with open cholecystectomy. subsequent infectious complications are often delayed, occurring in 0.1% to 2.9% of cases. we present the first reported case of salmonella enterica subphrenic abscess and empyema complicating dropped gallstones, treated with video-assisted thoracoscopic surgery (vats), and surgical retrieval. | 2010 | 20729699 |
salmonella-infected submandibular gland cyst: case report and review of the literature. | 2010 | 20727636 | |
short communication: antimicrobial effect of lactoferrin and its amidated and pepsin-digested derivatives against salmonella enteritidis and pseudomonas fluorescens. | the antimicrobial effect of bovine lactoferrin (lf) and its amidated and pepsin-digested derivatives, at concentrations varying from 0.25 to 20 mg/ml, against 3 salmonella enteritidis strains and 3 pseudomonas fluorescens strains was investigated. lactoferrin showed its maximum antimicrobial effect at 10 mg/ml against the 3 salmonella strains, with reductions ranging from 1.3 to 2.0 log units, and the 3 pseudomonas strains, with reductions ranging from 1.8 to 5.4 log units. in the case of amidat ... | 2010 | 20723671 |
salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class i integrons. | while considerable foodborne pathogen research has been conducted on conventionally produced broilers and turkeys, few studies have focused on free-range (organic) or pastured poultry. the current surveillance study was designed to isolate, identify and genetically characterize salmonella from pastured poultry farm environment and from retail samples. | 2010 | 20722876 |
analysis of gut immune-modulating activity of beta-1,4-mannobiose using microarray and real-time reverse transcription polymerase chain reaction. | beta-1,4-mannobiose (mnb) supplementation has been shown to prevent salmonella enteritidis infection in broilers by improving salmonella enteritidis clearance and increasing iga production. this study examined in detail the gut immunomodulatory activity of mnb using microarray and real-time quantitative pcr analysis. one-day-old chicks were orally administered 0.1% (wt/wt) mnb 3 times a week for 28 d. control birds received vehicle alone. body weights and fecal iga levels were monitored weekly. ... | 2010 | 20709974 |
production of monoclonal antibodies in a mouse model via lipopolysaccharide conjugates with synthetic polymers specific to salmonella enteritidis o antigen. | monoclonal antibodies (mabs) specific for lipopolysaccharide (lps) of salmonella enteritidis were evaluated in a model of lps conjugated synthetic polymer immunization of balb/c mice by conventional hybridoma method. nine hybridoma cell lines were determined as antibody positive against lps. the clone 5a8 secreting the highest antibody was selected for further characterization. evaluated results indicate that the synthetic polymer can be used as an effective adjuvant in immunization with lps, be ... | 2010 | 20707736 |
screening of antibacterial activity of mucus extract of snakehead fish, channa striatus (bloch). | the objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, channa striatus against selected human and fish pathogenic microbes. | 2010 | 20707287 |
[report on salmonella isolates in livestock, food and feed, received at the german national reference laboratory for salmonella during 2004-2008]. | the german national reference laboratory for salmonella receives salmonella isolates from diverse laboratories in germany. most of the salmonella strains originated from livestock and food.this report summarizes the studies of the german national reference laboratory on the prevalence of salmonella ssp. in livestock, food and feed for the years 2004-2008. in the past five years, the national reference laboratory received 23,949 salmonella isolates with s. enterica serovar typhimurium and s. ente ... | 2010 | 20690538 |
removal of salmonella enteritidis from commercial unpasteurized liquid egg white using pilot scale cross flow tangential microfiltration. | effectiveness of a cross flow microfiltration (mf) process for removal of a cocktail of salmonella enterica serovar enteritidis species from commercial unpasteurized liquid egg white (lew) from a local egg breaking plant, while maintaining its functional properties was evaluated. to facilitate mf, lew was wedge screened, homogenized and then diluted (1:2 w/w) with distilled water containing 0.5% sodium chloride. diluted unpasteurized lew was inoculated with five strains of s. enteritidis (atcc 4 ... | 2010 | 20688406 |
evaluation of pulsed electric fields technology for liquid whole egg pasteurization. | this investigation evaluated the lethal efficiency of pulsed electric fields (pefs) to pasteurize liquid whole egg (lwe). to achieve this aim, we describe the inactivation of salmonella enteritidis and the heat resistant salmonella senftenberg 775 w in terms of treatment time and specific energy at electric field strengths ranging from 20 to 45 kv/cm. based on our results, the target microorganism for this technology in lwe varied with intensity of the pef treatment. for electric field strengths ... | 2010 | 20688225 |