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identification of odors from overripe mango that attract vinegar flies, drosophila melanogaster.bioassays with a variety of overripe fruits, including mango, plum, pear, and grape, and their extracts showed that odors from overripe mango were most attractive to adult vinegar flies, drosophila melanogaster. combined gas chromatography-electroantennographic detection (gc-ead) analyses of solid-phase microextraction (spme) and tenax extracts of overripe mango odors showed that several volatile compounds, including ethanol, acetic acid, amyl acetate, 2-phenylethanol, and phenylethyl acetate el ...200312775150
the neuronal structure of the red nucleus in newborn guinea pigs.the preparations, stained according to the nissl and klüver-barrera methods, were used to describe the topography and morphology of the red nucleus (rn) as well as the structure of the rubral perikarya in newborn (p0) guinea pigs. the golgi impregnated preparations were used to distinguish types of neurons. rn is a uniform cell group and has the length from 740 to 860 microm. the nissl stained perikarya were classified into three categories: big, medium-sized and small (a, b, c, respectively). t ...200312769171
climatic factors governing plant phenological phases along a norwegian fjord.in the present project, the time of leaf budding and flowering, and partly also of fruit ripening, was studied over 3 years in different cultivated and native plants on a gradient along a western norwegian fjord about 300 km long, from oceanic to relatively continental regions. in the plants investigated, flowering of the red currant was most strongly favoured by oceanic conditions in the outermost part of the fjord. on the other hand, flowering of the apple was earliest in the middle district, ...200312750970
ntp toxicology and carcinogenesis studies of benzyl acetate (cas no. 140-11-4) in f344/n rats and b6c3f1 mice (gavage studies).benzyl acetate, a water-white liquid with a pear-like odor, is a natural constituent of several essential oils and flower absolutes extracted from jasmine, hyacinth, gardenia, tuberose, ylang-ylang, cananga, and neroli. commercial benzyl acetate, a liquid prepared synthetically from benzyl chloride, acetic acid, and triethylamine is used primarily as a component of perfumes for soaps and as a flavoring ingredient. this compound is practically insoluble in water but is miscible in alcohol and eth ...198612748678
communicative deficits in agenesis of the corpus callosum: nonliteral language and affective prosody.while some individuals with agenesis of the corpus callosum can perform normally on standardized intelligence tests, clinical observations suggest that they nevertheless have deficits in the domains of fluid and social intelligence. particularly important for social competence is adequate understanding and use of paralinguistic information. this study examined the impact of callosal absence on the processing of pragmatic and paralinguistic information. young adult males with agenesis of the corp ...200312735947
determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor.the aim was to experimentally evaluate the antioxidant capacity of different fresh aromatic herbs (field balm, marjoram, parsley, rosemary, sage, sweet basil), several varieties of olives from central italy ('carboncello', 'rosciolo', 'olivastro', 'coratello', 'leccino', 'frantoio') and several types of fresh fruit (apple, apricot, banana, cherry, fig, grape, medlar, melon, peach, pear, pineapple, plum, water melon, yellow plum) using a superoxide dismutase (sod) biosensor developed by the prese ...200312733012
isolation and characterization of four ethylene perception elements and their expression during ripening in pears (pyrus communis l) with/without cold requirement.pear (pyrus communis l.) are climacteric fruit: their ripening is associated with a burst of autocatalytic ethylene production. some late pear cultivars, such as passe-crassane (pc) require a long (80 d) chilling treatment before the fruit will produce autocatalytic ethylene and ripen. as the cold requirement is linked to the capacity to respond to ethylene (or its analogue, propylene), three pear cdnas homologous to the arabidopsis ethylene receptor genes at-etr1, at-ers1, and at-etr2, designat ...200312730273
the neuronal structure of the medial geniculate body in the pig--nissi and golgi study.the studies were carried out on the brains of adult pigs. the preparations were made by means of the golgi technique as well as the nissl and klüver-barrera methods. four types of neurons were described in the medial geniculate body (mgb) of the pig: 1. multipolar neurons (perikarya 30-45 microm) with rounded, oval or quadrangular perikarya from which arise 4-7 dendritic trunks. the dendrites divide dichotomically twice, may send out collaterals and give off ramifications. the dendritic branches ...200212725496
is angiomatosis an intrinsic pathohistological feature of massive osteolysis? report of an autopsy case and a review of the literature.we report here an autopsied patient who had died during the clinical course of massive osteolysis (mo), which is a rare chronic disease that begins insidiously and is characterized by progressive regional loss of bone. since the original description by gorham and stout in 1955, vascular proliferation, e.g., hemangiomatosis, has been considered to be the characteristic feature related to the pathogenesis. however, no such vascular changes were observed in the present patient. it was also importan ...200312715175
evaluation of an enzyme immunoassay for the detection of the insect growth regulator fenoxycarb in environmental and biological samples.a competitive enzyme-linked immunosorbent assay (elisa) for fenoxycarb was adapted for quantitative detection of this insect growth regulator in various environmental, agricultural, food and biological matrices. environmental samples were taken from soil and surface waters in hungary. the elisa enabled fenoxycarb detection in surface waters in the 1.1-125 ng ml(-1) concentration range without sample cleanup. in contrast, soil produced a strong matrix effect due to humic acids and other soil comp ...200312701701
bacteriocin serratine-p as a biological tool in the control of fire blight erwinia amylovora.fire blight, caused by the bacterium erwinia amylovora (burill winslow et al.), is the most important bacterial disease in european pear growing. it can cause a lot of damage in some countries on apple and on pear trees in orchards and also in the fruit tree nurseries. in belgium, the disease is present since 1972. control of fire blight in belgian fruit orchards is made on a broad basis of measurements in and around the fruit trees. the use of an antibiotic is allowed for application only durin ...200212701444
determination of neonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry.a rapid and simple extraction method for the simultaneous analysis of five neonicotinoid insecticides has been developed. twelve different fruit and vegetable matrixes were extracted with methanol and cleaned up using a graphitized carbon solid phase extraction cartridge loading with a 20% methanol solution. the concentrated eluate after methanol elution was then analyzed for pesticide residues by liquid chromatography/mass spectrometry in the apci positive mode. the five pesticides including ni ...200312696927
non-conventional methods for the control of post-harvest pear diseases.pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. among the fungal diseases, blue mold caused by penicillium expansum, grey mould caused by botrytis cinerea, mucor rot caused by mucor piriformis are common on pear fruits. other (weak) pathogens like phialophora malorum, alternaria spp., and cladosporium herbarum tend to infect wounds and senescent fruits. a post-harvest fungicide treatment can reduce decay but effectiveness decrea ...200312694440
cloning, expression, and characterization of sorbitol transporters from developing sour cherry fruit and leaf sink tissues.the acyclic polyol sorbitol is a primary photosynthetic product and the principal photosynthetic transport substance in many economically important members of the family rosaceace (e.g. almond [prunus dulcis (p. mill.) d.a. webber], apple [malus pumila p. mill.], cherry [prunus spp.], peach [prunus persica l. batsch], and pear [pyrus communis]). to understand key steps in long-distance transport and particularly partitioning and accumulation of sorbitol in sink tissues, we have cloned two sorbit ...200312692316
bacteriophages of erwinia amylovora.fifty bacteriophage isolates of erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the niagara region of southern ontario and the royal botanical gardens, hamilton, ontario. forty-two phages survived the isolation, purification, and storage processes. the majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fire blight symptoms. only five phages were isolated from infected aerial tissue in pear and apple orchar ...200312676693
cutaneous vascular malformations in disappearing bone (gorham-stout) disease. 200312672746
molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from malus domestica and pyrus communis cultivars and the consequences for flavonoid metabolism.treatment with the dioxygenase inhibitor prohexadione-ca leads to major changes in the flavonoid metabolism of apple (malus domestica) and pear (pyrus communis) leaves. accumulation of unusual 3-deoxyflavonoids is observed, which have been linked to an enhanced resistance toward fire blight. the committed step in this pathway is the reduction of flavanones. crude extracts from leaves are able to perform this reaction. there was previous evidence that dfr enzymes of certain plants possess additio ...200312667486
instability of short-sequence dna repeats of pear pathogenic erwinia strains from japan and erwinia amylovora fruit tree and raspberry strains.an array of short-sequence dna repeats (ssrs) occurs in the plasmid pea29 of the fire blight pathogen erwinia amylovora. a large number of "fruit tree" strains, mainly from central and western europe, were screened for their ssr numbers, and the analyses were extended to five raspberry strains from north america and six pear pathogenic erwinia strains from japan. the repeat attacaga present in all e. amylovorastrains was found to be reiterated 3 to 15 times. the japanese strains contained the ma ...200312655400
localized graft incompatibility in pear/quince (pyrus communis/cydonia oblonga) combinations: multivariate analysis of histological data from 5-month-old grafts.to characterize the structural events associated with incompatibility of graft development, we conducted a histological study of compatible and incompatible pear/pear and pear/quince grafts that had been grown for five months in a greenhouse. multivariate analysis of histological data describing the structure of the graft union allowed us to discriminate between compatible and incompatible combinations before either macroscopic examination or qualitative microscopic examination differences betwe ...199912651320
activation of three pathogen-inducible promoters of tobacco in transgenic pear (pyrus communis l.) after abiotic and biotic elicitation.in order to improve pear resistance against fire blight caused by erwinia amylovora, a search for promoters driving high-level expression of transgenes specifically in response to this bacterial pathogen has been undertaken. we have examined the ability of hsr203j, str246c and sgd24 tobacco (nicotiana tabacum l.) promoters to drive expression of the uida reporter gene in pear. transgenic pear clones were obtained by agrobacterium tumefaciens-mediated transformation. beta-glucuronidase activity w ...200312624768
involvement of three pathogenicity factors of erwinia amylovora in the oxidative stress associated with compatible interaction in pear.erwinia amylovora, the causal agent of fire blight of maloideae, induces in its susceptible host plants an oxidative burst as does an incompatible pathogen. in this paper we present evidence that the elicitation of this phenomenon is the result of the combined action of two hrp effectors of the bacteria, hrpn and dspa. we also confirmed that desferrioxamine, the siderophore of e. amylovora, is necessary for the bacteria to tolerate high levels of hydrogen peroxide. two other pathogenicity factor ...200312606057
genetic linkage maps constructed by using an interspecific cross between japanese and european pears.genetic linkage maps of the european pear ( pyrus communis l.) cultivar 'bartlett' and the japanese pear ( pyrus pyrifolia nakai) cultivar 'housui' were constructed based on aflps, ssrs from pear, apple and prunus, isozymes and phenotypic traits by using their f(1) progenies. the map of the female parent bartlett consisted of 226 loci including 175 aflps, 49 ssrs, one isozyme and one s locus on 18 linkage groups over a total length of 949 cm, while that for 'housui' contained 154 loci including ...200212582866
comparative analysis of chloroplast dna in pyrus species: physical map and gene localization.a physical map of chloroplast dna (cpdna) of pear [pyrus ussuriensis var. hondoensis (nakai et kikuchi) rehder] was constructed using five restriction enzymes, sali, xhoi, bamhi, saci and psti. this information will make it possible to investigate the phylogenetic relationships between pyrus species. pear cpdna was found to be a circular molecule with a total size of about 156 kb in which two inverted repeats of 24.8 kb divide the molecule into small (17 kb) and large (90 kb) single-copy regions ...200312582856
west & west syndrome--a historical sketch about the eponymous doctor, his work and his family.the british surgeon william james west has not left a tremendous literary or scientific work as many of his contemporaries did. for this reason only a little has been known about him and the fate of his family for decades, even though the eponym was created in the 1960s. only in 1990 was a first biography published and later on supplemented. if his son had not suffered from the syndrome, which later on was named after him, he would not have published the first description of the west syndrome in ...200312581804
ethylene is required for both the initiation and progression of softening in pear (pyrus communis l.) fruit.in order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-mcp), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. the propylene treatment at the pre-ripe stage stimulated ethylene production and flesh softening while the 1-mcp treatment at the same stage markedly retarded the initiation of the ripening-related events ...200312554720
the cytoskeleton of giardia lamblia.giardia lamblia is a ubiquitous intestinal pathogen of mammals. evolutionary studies have also defined it as a member of one of the earliest diverging eukaryotic lineages that we are able to cultivate and study in the laboratory. despite early recognition of its striking structure resembling a half pear endowed with eight flagella and a unique ventral disk, a molecular understanding of the cytoskeleton of giardia has been slow to emerge. perhaps most importantly, although the association of giar ...200312547343
[effect of nos positive neurons in rats with learning-remembering barrier].to study the variation of nitric oxide synthase (nos) neurons in rats with learning-remembering barrier by immunohistochemistry and histochemistry. the number of nos neurons decreased apparently in hippocampus ca1-4 of rats with learning-remembering barrier, there were lots of new nos neurons in dentate gyrus granular cell grays and pear-shaped zone's cortex. it is suggested that nos neurons of hippocampus exert very important action in rat's learning remembering; new nos neurons in pear-shaped ...200112536726
[favourable effect of a standard elimination diet on the behavior of young children with attention deficit hyperactivity disorder (adhd): a pilot study].to determine whether a standard elimination diet can decrease the adhd-symptoms in a heterogeneous group of young children with adhd.200212532668
molecular characterization of natural erwinia pyrifoliae strains deficient in hypersensitive response.from necrotic tissue of a nashi pear tree, 24 erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. the defective gene hrpl was complemented with intact genes from e. pyrifoliae and erwinia amylovora.200312514060
effect of prickly pear (opuntia robusta) on glucose- and lipid-metabolism in non-diabetics with hyperlipidemia--a pilot study.besides others pectin, a soluble fibre, has been reported to be effective in lowering cholesterol levels in both animals and man with hyperlipidemia as well as being able to slow carbohydrate absorption and hence reduce the postprandial rise in blood glucose and serum insulin in patients with type-ii diabetes. aim of this pilot study was to assess the effect of prickly pear consumption on glucose- and lipid metabolism.200212503475
gorham-stout disease of the shoulder girdle and cervico-thoracic spine: fatal course in a 65-year-old woman.a case of gorham-stout disease of the shoulder girdle and cervico-thoracic spine in a 65-year-old woman is described. the patient presented with progressive neurologic symptoms, pain, and deformities of the cervico-thoracic spine as well as of her left shoulder following a traumatic shoulder luxation. since the patient had a history of uterine carcinoma, the current disease was clinically difficult to differentiate from osteolytic metastases. the results of the clinical, radiological and histopa ...200212483436
genetics of biosynthesis and structure of the capsular exopolysaccharide from the asian pear pathogen erwinia pyrifoliae.erwinia pyrifoliae is a novel bacterial pathogen, which causes asian pear blight and is related to erwinia amylovora, the causative agent of fire blight. e. pyrifoliae produces exopolysaccharide (eps) related to amylovoran in its sugar composition and sugar linkages. this was shown by degradation of the eps with a viral depolymerase, and by methylation analysis and esi/ms. the structure of the repeating units was confirmed by (1)h-nmr spectra. the eps of e. pyrifoliae carried side chains, which ...200212480905
benzoquinone, the substance essential for antibacterial activity in aqueous extracts from succulent young shoots of the pear pyrus spp.aqueous extracts of the tissue of succulent young shoots of the pear pyrus spp. exhibited strong antibacterial activity against the bacterium erwinia amylovora bv. 4. this activity was investigated quantitatively by a newly developed bioassay method. it was found that the activity changed with the age of the tissue. extracts of the youngest leaves and stems from the shoot tops showed the strongest activity, and the activity decreased with age of the leaves and stems. the activity also changed wi ...200312475625
characterization of erwinia amylovora strains using random amplified polymorphic dna fragments (rapds).the genetic diversity among 16 strains of erwinia amylovora, chosen to represent different host plant origins and geographical regions, was investigated by rapd analysis. one strain of erwinia herbicola and one of agrobacterium vitis were used as outgroups. ninety-eight different rapd fragments were produced by polymerase chain reaction amplification with six different 10-mer primers. rapd banding profiles were found that enabled the erw. amylovora strains to be distinguished from one another. c ...199712455904
antioxidant and antiproliferative activities of common fruits.consumption of fruits and vegetables has been associated with reduced risk of chronic diseases such as cardiovascular disease and cancer. phytochemicals, especially phenolics, in fruits and vegetables are suggested to be the major bioactive compounds for the health benefits. however, the phenolic contents and their antioxidant activities in fruits and vegetables were underestimated in the literature, because bound phenolics were not included. this study was designed to investigate the profiles o ...200212452674
characterization of serracin p, a phage-tail-like bacteriocin, and its activity against erwinia amylovora, the fire blight pathogen.serratia plymithicum j7 culture supernatant displayed activity against many pathogenic strains of erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against klebsiella pneumoniae, serratia liquefaciens, serratia marcescens, and pseudomonas fluorescens. this activity increased significantly upon induction with mitomycin c. a phage-tail-like bacteriocin, named serracin p, was purified from an induced culture supernatant of s. plymith ...200212406768
comparison of the nutrient content of fresh fruit juices vs commercial fruit juices.to compare the types and quantities of carbohydrate, electrolytes, ph and osmolarity of fresh fruit juices and commercial fruit juices.200212403253
distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations.total and free phenolic contents of 16 commonly consumed fruits (comprising 9 apples, 4 pears, and one each of peach, plum, and kiwi fruit cultivars) were measured by folin-ciocalteu assay. total phenol contents varied from 272 to 475 mg of cte/100 g of fresh weight. of the apple cultivars studied, braeburn and empire had the highest and lowest total phenol content, respectively. the apple cultivars ranked in the following decreasing order: braeburn > red delicious > crisp pink > granny smith > ...200212381107
a pistil-specific thaumatin/pr5-like protein gene of japanese pear (pyrus serotina): sequence and promoter activity of the 5' region in transgenic tobacco.the genomic clone encoding the pistil-specific thaumatin/pr5-like protein (pstl1) was isolated from japanese pear (pyrus serotina). sequence analysis showed that the genomic clone contained the 5'-flanking sequence of 2.4 kb, the 3'-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. a sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5'-flanking region of the pstl1 gene. the 2. ...200212369614
lesions in subclinical paratuberculosis of goats are associated with persistent gut-associated lymphoid tissue.the organized gut-associated lymphoid tissue (the peyer's patches [pps] of domestic ruminants) is an important site of lesions caused by mycobacterium avium subsp. paratuberculosis. to investigate the association between pp morphology and the lesions of paratuberculosis in goats, two experiments were performed. five healthy kids aged 4-5 weeks were examined and the morphology of organized lymphoid tissue in the small intestine was described. morphological similarities were observed between the i ...200212354531
a new class of regulatory genes underlying the cause of pear-shaped tomato fruit.a common, recurring theme in domesticated plants is the occurrence of pear-shaped fruit. a major quantitative trait locus (termed ovate) controlling the transition from round to pear-shaped fruit has been cloned from tomato. ovate is expressed early in flower and fruit development and encodes a previously uncharacterized, hydrophilic protein with a putative bipartite nuclear localization signal, von willebrand factor type c domains, and an approximately equal 70-aa c-terminal domain conserved in ...200212242331
protein synthesis and breakdown during heat shock of cultured pear (pyrus communis l.) cells.cultured pear (pyrus communis l. cv passe crassane) cells were subjected to temperatures of 39, 42, and 45[deg]c. heat-shock protein (hsp) synthesis was greater at 30[deg]c than at temperatures above 40[deg]c and continued for up to 8 h. both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. polysome levels decreased immediately when cells were placed at 39 or 42[deg]c, although at 39[deg]c the levels began to recove ...199412232178
effect of cations on effective permeability of leaf cuticles to sulfuric acid.many plants are exposed to prolonged episodes of anthropogenic acid precipitation with ph values of 4 or less, but there is little evidence of widespread direct damage to the plant cells. acids appear to permeate leaf cuticle via charged pores, which act as a fixed buffer that delays but does not stop acid movement. we investigated the effect of cations on the movement of protons through astomatous isolated leaf cuticles of pear (pyrus communis l.) and rough lemon (citrus limon [l.] burm. fils c ...199312231931
patterns of effective permeability of leaf cuticles to acids.plants in the field are frequently exposed to anthropogenic acid precipitation with ph values of 4 and below. for the acid to directly affect leaf tissues, it must pass through the leaf cuticle, but little is known about the permeability of cuticles to protons, or about the effect of different anions on this permeability. we investigated the movement of protons through isolated astomatous leaf cuticles of grapefruit (citrus x paradisi macfady.), rough lemon (citrus limon [l.] burm. fils cv ponde ...199312231681
in vitro prenylation of the small gtpase rac13 of cotton.previous work (d.p. delmer, j. pear, a. andrawis, d. stalker [1995] mol gen genet 248: 43-51) has identified a gene in cotton (gossypium hirsutum), rac13, that encodes a small, signal-transducing gtpase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. since rac13 may be important in signal transduction pathway(s), regulating the onset of fiber secondary wall synthesis, we continue to characterize rac13 by determining its ability to underg ...199612226460
alpha-glucosidase inhibitor from the seeds of balsam pear (momordica charantia) and the fruit bodies of grifola frondosa.alpha-glucosidase inhibitory activities were found in aqueous methanol extracts of the seeds of momordica charantia and the fruit bodies of grifola frondosa. an active principle against the enzyme prepared from rat small intestine acetone powders was isolated and characterized. the structure of the isolated compound was identified as d-(+)-trehalose by fdms, 1h-, 13c-nmr, and [alpha]d measurements. the inhibitory activity of trehalose was compared with 1-deoxynojirimycin. trehalose showed 45% in ...200212224646
pear transformed with a lytic peptide gene for disease control affects nontarget organism, pear psylla (homoptera: psyllidae).the biology and behavior of pear psylla, cacopsylla pyricola foerster, on a transgenic clone of 'bartlett' pear, pyrus communis l., containing a synthetic antimicrobial gene, d5c1, was compared with that of a nontransgenic parental clone to determine whether there were any nontarget effects. the gene construct also contained the marker gene nptii (aminoglycoside 3'-phosphotransferase ii) that encodes for antibiotic resistance to identify transformed plants. the purpose of the original transforma ...200212216823
modulation of antioxidant compounds in organic vs conventional fruit (peach, prunus persica l., and pear, pyrus communis l.).despite the increasing interest in organic products, knowledge about how different levels of fertilization affect nutritionally relevant components is still limited. the concentration of polyphenols and the activity of polyphenoloxidase (ppo), together with the content in ascorbic acid, citric acid, and alpha- and gamma-tocopherol, were assayed in conventional and organic peach (prunus persica l., cv. regina bianca) and pear (pyrus communis l., cv. williams). 2-thiobarbituric acid reactive subst ...200212207491
a novel multiplex rt-pcr probe capture hybridization (rt-pcr-elisa) for simultaneous detection of six viroids in four genera: apscaviroid, hostuviroid, pelamoviroid, and pospiviroid.a rapid and sensitive assay was developed for the detection and identification of viroids by standard or multiplex reverse transcription-polymerase chain reaction (rt-pcr)-probe capture hybridization (rt-pcr-elisa). the assay was applied successfully for the detection and identification of the following six viroid species from infected tissues: potato spindle tuber viroid (pospiviroid), peach latent mosaic viroid (pelamoviroid), apple scar skin viroid (apscaviroid), apple dimple fruit viroid (ap ...200212176148
an allergen from olea europaea pollen (ole e 7) is associated with plant-derived food anaphylaxis.several cross-reacting proteins have been identified as responsible of the co-occurrence of pollinosis and plant-derived food allergy. this association has been mainly described in the birch-apple syndrome but other pollens such as olea europaea and other fruits may also contain homologous proteins.200212173271
the role of horizontal categorization in retroactive and proactive interference.four paired-associate experiments with a total n of 291 participants investigated the effects of horizontal categorization on retroactive and proactive interference. (exclusively) horizontal categorization means that unique categorical relationships hold across the a-b and a-c stimulus-response pairs of successive word lists (e.g., fruit--pear, river--thames, in list 1; and fruit--plum, river--wolga, in list 2). experiment 1 found no significant amounts of interference with this type of list org ...200212152363
[massive post-traumatic osteolysis of the thorax and cervical spine--gorham-stout syndrome]. 200212142985
composition of phenolic compounds in a portuguese pear (pyrus communis l. var. s. bartolomeu) and changes after sun-drying.the composition of phenolic compounds of a portuguese pear cultivar (pyrus communis l. var. s. bartolomeu) was determined by hplc after thioacidolysis. the average concentration of phenolic compounds in pear harvested at commercial maturity stage was 3.7 g per kg of fresh pulp. procyanidins were the predominant phenolics (96%), with a mean degree of polymerization (mdp) of 13-44; hydroxycinnamic acids (2%), arbutin (0.8%), and catechins (0.7%) were also present. the most abundant monomer in the ...200212137473
remarkable variability of apple mosaic virus capsid protein gene after nucleotide position 141.eight new sequences of european isolates from almond, apple, hop, prune and pear of the apple mosaic ilarvirus (apmv) capsid protein gene are presented. a consensus sequence was established as having 654 nucleotides (nt) and two american and two european isolates were identified to have insertions 6 to 15 nucleotides after nt position 141. the insertion resulted in the american isolate a inframeshift repaired with two point insertions 17 and 68 nt downstream. the rna around the insertion point c ...200212111408
cultivation of babesia and babesia-like blood parasites: agents of an emerging zoonotic disease.babesia and its close relatives are members of a group of organisms called piroplasms, a name which comes from their pear-shaped outlines. long associated with blood diseases of cattle and other mammals, members of the genus babesia have been recognized since the 1950s as infectious agents in humans. species of this protozoan blood parasite that have routinely been isolated from mice (b. microti) or cattle (b. divergens) have also been isolated from humans. in addition to these familiar species, ...200212097245
characterization of bacillus strains from apple and pear trees in south africa antagonistic to erwinia amylovora.in order to find reasons for the absence of fire blight in most countries of the southern hemisphere, bark samples from apple and pear trees in orchards of the western cape region in south africa were extracted for bacteria which could be antagonistic to erwinia amylovora. screening was done in the late growth season and mainly gram-positive bacteria were isolated. approximately half of them produced growth inhibition zones on a lawn of e. amylovora. most isolates were classified as bacillus meg ...200212076820
neonatal pneumonia caused by trichomonas vaginalis.the authors present two cases of newborn babies infected by trichomonas vaginalis (hereafter referred to as t. vaginalis) and suffering from severe congenital breathing difficulties and needing artificial respiration. microscopic examination of the tracheal discharge revealed characteristically moving, flagellated, pear-shaped unicellular organisms. cultures on cplm medium proved the presence of t. vaginalis. during pregnancy the mothers' clinical status was negative and both of them mentioned l ...200212073821
[massive gorham-stout osteolysis of the temporal bone and the craniocervical transition].massive osteolysis gorham-stout is a rare, benign but locally aggressive angiomatosis which results in destruction and resorption of bone. the etiology and pathogenesis are undefined. the occurrence of the disease in the skull base is uncommon. a 54-year-old female presented with isolated, one-sided surditas. eight years before the patient underwent surgery and radiation therapy for treatment of hypopharyngeal cancer. a transtemporal biopsy was taken and a highly vascularized, cystic lesion with ...200212063694
flavonoid compounds from pyrus communis l. flowers.five flavonoid glycosides: kaempferol 3-o-beta-d-glucopyranoside (iv), isorhamnetin 3-o-beta-d-glucopyranoside (v), isorhamnetin 3-o-beta-d-(6''-o-alpha-l-rhamnopyranosyl)-glucopyranoside (viii), 8-methoxykaempferol 3-o-beta-d-(2''-o-alpha-d-glucopyranosyl)-glucopyranoside (vi), 8-methoxykaempferol 3-o-beta-d-(2''-o-alpha-l-rhamnopyranosyl)-glucopyranoside (vii) and chlorogenic acid (ix) were isolated from the flowers naturally growing pyrus communis l. they were identified by chemical and spect ...200912026113
a conditionally dispensable chromosome controls host-specific pathogenicity in the fungal plant pathogen alternaria alternata.the filamentous fungus alternaria alternata contains seven pathogenic variants (pathotypes), which produce host-specific toxins and cause diseases on different plants. previously, the gene cluster involved in host-specific ak-toxin biosynthesis of the japanese pear pathotype was isolated, and four genes, named akt genes, were identified. the akt homologs were also found in the strawberry and tangerine pathotypes, which produce af-toxin and act-toxin, respectively. this result is consistent with ...200212019223
the antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin c composition.recent studies are emphasising the importance and putative modes of action of specific flavonoids as bioactive components of the diet in in vivo and in vitro models. thus, it is important to have a clear idea of the major phenolic families of which fruit and vegetables are comprised and the levels contained therein. regularly consumed fruit and vegetables of mixed varieties available on the uk market were analysed for the composition of the major individual phenolic components. the total phenoli ...200211999391
gorham disease of the orbit.to report a case of gorham disease, also known as vanishing bone disease, of the orbit.200211992885
identification of betalains from yellow beet (beta vulgaris l.) and cactus pear [opuntia ficus-indica (l.) mill.] by high-performance liquid chromatography-electrospray ionization mass spectrometry.betaxanthins, the yellow-orange water-soluble pigments from yellow beet (beta vulgaris ssp. vulgaris cv. bejo zaden) and cactus pear (opuntia ficus-indica cv. gialla) have been investigated using an hplc system compatible with mass spectrometry. five novel betaxanthins were found and characterized as the immonium adducts of betalamic acid with serine, gamma-aminobutyric acid, valine, isoleucine, and phenylalanine. to enable concentration of betalain samples, desalting was performed by solid-phas ...200211929288
natural products used for diabetes.to review the efficacy and safety of natural products commonly used for diabetes.200211926665
food consumption and adipose tissue ddt levels in mexican women.this article analyzes food consumption in relation to levels of dde (the principal metabolite of ddt) in the adipose tissue of 207 mexican women residing in states with high and low exposure to ddt. data on the women's dietary habits and childbearing history were obtained from a personal interview. adipose tissue dde levels were measured by gas-liquid chromatography and compared by analysis of variance (anova) and multiple linear regression. adipose tissue dde levels increased significantly with ...200211923886
connections of the ventral telencephalon and tyrosine hydroxylase distribution in the zebrafish brain (danio rerio) lead to identification of an ascending dopaminergic system in a teleost.we studied the connections and catecholaminergic organization of the subpallium in the zebrafish, in particular to demonstrate the origin of the ascending dopaminergic system of teleosts, by using the tracers dii or biocytin in combination with tyrosine hydroxylase (th) immunohistochemistry. retrogradely labeled cells were found in the olfactory bulb, the area dorsalis telencephali, the preoptic region, the dorsal and ventral thalamus, the posterior tubercle, the preglomerular region, and the me ...201311922994
synthesis of theaflavin from epicatechin and epigallocatechin by plant homogenates and role of epicatechin quinone in the synthesis and degradation of theaflavin.oxidation products of (-)-epicatechin and (-)-epigallocatechin by treatment with homogenates of 62 plants belonging to 49 families were compared. forty-six plants were capable of synthesizing theaflavin, a black tea pigment, regardless of whether they contained catechins. loquat, japanese pear, and blueberry had activities higher than that of fresh tea leaves after 5 h of treatment; furthermore, these plants oxidized theaflavin to theanaphthoquinone. an additional new metabolite, dehydrotheasine ...200211902970
hydrolytic activity and ultrastructural changes in fruit skins from two prickly pear (opuntia sp.) varieties during storage.the activity of four cell wall hydrolases, pectinmethylesterase (pme), polygalacturonase (pg), cellulase, and beta-galactosidase (beta-gal), was measured in fruit skins of two prickly pear varieties, naranjona and charola, during storage at 18 degrees c and 85-95% relative humidity (rh). in naranjona (opuntia ficus indica), of short postharvest life (ca. 2 weeks), pg, cellulase, and beta-gal increased their activity more than twice, whereas pme activity tended to increase only slightly during st ...200211879058
comparative analysis of phenolic acids in mistletoe plants from various hosts.phenolic acids present in mistletoe plants collected from various hosts were analysed with the use of hplc. the following numbers of compounds were found in the mistletoe plant material gathered from respective hosts: sorbus aucuparia- 12 compounds; acer plantanoides--14 compounds: malus domestica, pyrus communis and populus nigra--13 compounds each; quercus robur--15 compounds. altogether 21 phenolic acids were chromatographically identified in the tested material. the compounds were either fre ...201211876445
high-performance liquid chromatographic analysis of free amino acids in fruit juices using derivatization with 9-fluorenylmethyl-chloroformate.a simple, rapid, and reliable reversed-phase high-performance liquid chromatographic method for the analysis of 16 amino acids of main interest in commercial fruit juices (pear, orange, grapefruit, pineapple, peach, and apricot) is described. no sample cleanup is required. the ph of the fruit juices is adjusted to alkaline value (8.5) using 200 mm borate buffer, then amino acid is converted to stable derivatives using 9-fluorenylmethyl-chloroformate. the excess of derivatization reagent is remov ...200211866381
presence of thyrotropin-releasing-hormone-immunoreactive (trhir) amacrine cells in the retina of anuran and urodele amphibians.the presence of thyrotropin-releasing-hormone-immunoreactive (trh-ir) amacrine cells in the retina of amphibians is reported for the first time. the anuran and urodele retinas studied exhibit major differences in the distribution of trh-ir cells. in the two urodele species investigated, most trh-ir amacrine cells were located in the ganglion cell layer (gcl). these pear-shaped cells originate a dense trh-ir dendritic plexus in strata 4-5 of the inner plexiform layer (ipl). a small number of trh- ...200211814410
[epiphytic phase of erwinia amylovora and pseudomonas syringae pv. syringae on orchard weeds].epiphyte phase of phytopathogenic bacteria erwinia amylovora and pseudomonas syringae pv. syringae on the fruit garden weeds has been studied. it has been shown that healthy weeds of the fruit-tree stands can be an ecologic niche for erwinia amylovora and pseudomonas syringae pv. syringae which gives them an opportunity to survive as epiphytes. strains of pseudomonas syringae pv. syringae were isolated from seven studied weeds (47-49%) during the whole vegetation period (march-october). strains ...200111785263
overwintering of codling moth (lepidoptera: tortricidae) larvae in apple harvest bins and subsequent moth emergence.codling moths, cydia pomonella (l.), have long been suspected of emerging from stacks of harvest bins in the spring and causing damage to nearby apple and pear orchards. with increased use of mating disruption for codling moth control, outside sources of infestation have become more of a concern for growers using pheromone based mating disruption systems. studies were designed to provide information on bins as a source of codling moth and the pattern of codling moth emergence from stacks of bins ...200111777057
evaluation of the antioxidant properties of mediterranean and tropical fruits compared with common food additives.several mediterranean and tropical fruits have been analyzed in order to assess their antioxidant activity compared with that of common food additives (butylated hydroxyanisole [bha], butylated hydroxytoluene [bht] and propyl gallate). among mediterranean fruits, red grape and plum were more effective (p < 0.05) scavengers of peroxyl radicals than bha, bht, and propyl gallate. of the tropical fruits, banana was the most effective scavenger of peroxyl radicals. mediterranean and tropical fruits s ...200111770635
phenological modifications in plants by various edaphic factors.various mechanical, chemical and physical soil analyses were carried out, in addition to weather observations, for 3 years at several sites along an oceanic-continental gradient in a fjord district in western norway. all the environmental factors observed were correlated with the spring and a few late-season phenophases of many native and cultivated woody plants and some herbs by simple, linear correlations and by stepwise multiple and partial analyses. different techniques were used to try and ...200111769320
biological control of postharvest pear diseases using a bacterium, pantoea agglomerans cpa-2.epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against penicillium expansum on pears. from 247 microorganisms tested for antagonistic properties against p. expansum, a bacterium strain identified as pantoea agglomerans (cpa-2) was selected. this bacterium was very effective against botrytis cinerea, p. expansum and rhizopus stolonifer. complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x ...200111759762
cellular and humoral mechanisms of osteoclast formation and bone resorption in gorham-stout disease.gorham-stout disease (gsd) is a rare, massively osteolytic condition which is associated with increased vascularity and an increase in osteoclast numbers. to determine the cellular and humoral mechanisms underlying the increase in osteoclast numbers and osteolysis in gsd, this study analysed circulating osteoclast precursor numbers and sensitivity to osteoclastogenic factors in a gsd patient and age/sex-matched controls. monocytes were cultured with m-csf (25 ng/ml) and rankl (30 ng/ml) and oste ...200111745700
expression of antisense snrk1 protein kinase sequence causes abnormal pollen development and male sterility in transgenic barley.a chimaeric gene was constructed comprising a wheat high molecular weight glutenin subunit gene promoter, a 304-bp sucrose non-fermenting-1-related (snrk1) protein kinase sequence in the antisense orientation, and the cauliflower mosaic virus 35s rna gene terminator. transgenic barley plants containing the antisense snrk1 chimaeric gene were produced by particle bombardment of barley immature embryos with the aim of obtaining plants expressing the antisense snrk1 sequence in the seeds. despite t ...200111737780
cutaneous and gastrointestinal tract hemangiomas associated with disappearing bones: gorham syndrome. 200111737444
trichorhinophalangeal syndrome type i: clinical and molecular characterization of 3 members of a family and 1 sporadic case.trichorhinophalangeal syndrome type i (trps i) is a rare autosomal dominant disorder clinically characterized by sparse and slow-growing hair, pear-shaped nose, elongated philtrum, thin upper lip, and bone deformities, in particular, cone-shaped epiphyses of the phalanges. very recently, the responsible gene trps1 has been cloned on human chromosome 8q24.200111708946
molecular comparison of pathogenic bacteria from pear trees in japan and the fire blight pathogen erwinia amylovora.several strains of the genus erwinia, which were isolated in japan from pear trees with necrotic symptoms that resembled fire blight, and tentatively identified as erwinia amylovora, were reinvestigated for their relationship to the fire blight pathogen. these isolates produced ooze on slices of immature pears and were mucoid on mm2cu agar plates, but did not synthesize levan and did not give the expected pcr signals with several primer pairs specific for erwinia amylovora. the isolates tested p ...200111700346
a sensory neuron subpopulation with unique sequential survival dependence on nerve growth factor and basic fibroblast growth factor during development.we characterized a subpopulation of dorsal root ganglion (drg) sensory neurons that were previously identified as preferential targets of enkephalins. this group, termed p-neurons after their "pear" shape, sequentially required nerve growth factor (ngf) and basic fibroblast growth factor (bfgf) for survival in vitro during different developmental stages. embryonic p-neurons required ngf, but not bfgf. ngf continued to promote their survival, although less potently, up to postnatal day 2 (p2). co ...200111698599
a new type of exocrine gland and its function in mass recruitment in the ant cylindromyrmex whymperi (formicidae, cerapachyinae).workers of the ant cylindromyrmex whymperi display mass trail recruitment. bioassays show that the trail pheromone originates from a unique gland between abdominal sternites 6 and 7. the gland has a hitherto unknown structural organization. upon leaving the secretory cell, the duct cell widens to form a sclerotized pear-shaped reservoir chamber, lined with multiple duct cells. each duct thus forms a miniature reservoir for the secretions of each single secretory cells, a novel structural arrange ...200111688416
detection of four apple viruses by multiplex rt-pcr assays with coamplification of plant mrna as internal control.two multiplex rt-pcr assays with specific coamplification of plant mrna as an internal control from total nucleic acids are described for the parallel detection of apple chlorotic leaf spot virus, apple stem pitting virus, apple mosaic virus and apple stem grooving virus. all are important economically and common pathogens in commercial apple and pear cultivars, except for apple mosaic virus. four virus specific primer pairs and one primer pair which allows the specific amplification of mrna of ...200211684306
springtime pollinosis and oral allergy syndrome in sapporo.the purpose of this present study was to investigate pollinosis in the spring and oral allergy syndrome (oas) in sapporo to utilize it for future treatment.200111683343
with the ambulance service in france: the wartime letters of william gorham rice, jr. 198111639040
phenylcoumaran benzylic ether and isoflavonoid reductases are a new class of cross-reactive allergens in birch pollen, fruits and vegetables.we investigated the biochemical function of the birch pollen allergen bet v 6 and its role in the ige-cross-reactivity between birch pollen and plant foods, and characterized pyr c 5, a bet v 6-related food allergen, from pear; the proteins were expressed as his-tag fusion proteins in eschershia coli and purified by ni-chelate affinity chromatography under native conditions. nonfusion proteins were obtained by factor xa protease treatment. the highest degree of amino-acid sequence identity of py ...200111606193
seasonal occurrence of specialist and generalist insect predators of spider mites and their response to volatiles from spider-mite-infested plants in japanese pear orchards.in two adjacent japanese pear orchards (orchards i and 2), we studied the seasonal occurrence of the kanzawa spider mite. tetranychus kanzawai, and its predators. also the response of these predators to the volatiles from kidney bean plants infested with t kanzawai was investigated using trap boxes in orchard 1. the mite density in orchard 1 was unimodal. with one peak at the end of august. in this orchard, population development of the specialist insect predators, scolothrips takahashii, oligot ...200111603732
isolation and identification of mycoplasma genitalium from high risk populations of sexually transmitted diseases in china.to investigate mycoplasma genitalium (mg) infection in high risk populations of sexually transmitted diseases (stds) in china by means of culture, mg-dna detection, observation under an electron microscope (em) and an atomic force microscope (afm).199911601323
methylamine in human urine.methylamine is the simplest aliphatic amine found in human urine. in the body it is thought to play a significant part in central nervous system disturbances observed during renal and hepatic disease and also has a role in general toxicity caused by oxidative stress. the present study provides data on the daily urinary excretion of methylamine in a population of unrelated healthy volunteers and investigates a variety of food substances as potential dietary sources.200111580915
pesticide applications of copper on perennial crops in california, 1993 to 1998.inorganic copper is used as a broad-spectrum fungicide and bacteriocide on a variety of agricultural crops. after application, the copper residue typically accumulates in the upper 15 cm of soil. data from the california pesticide use reports were used to estimate the augmentation of copper in the soil that resulted from pesticide applications for the six years from 1993 to 1998 on 12 crops that are grown without rotation. the estimated mean mg cu kg(-1) soil added to the upper 15 cm during the ...200111577894
attractants from bartlett pear for codling moth, cydia pomonella (l.), larvae.the alkyl ethyl and methyl esters of (2e,4z)-2,4-decadienoic acid found in head-space samples of ripe bartlett pear (pyrus communis l.) stimulated a response from neonate larvae of the codling moth (cm), cydia pomonella (l.), in both static-air petri-plate and in upwind y-tube and straight-tube olfactometer bioassays. in comparison with the known cm neonate attractant, (e,e)-alpha-farnesene, ethyl (2e,4z)-2,4-decadienoate was attractive at 10-fold and 1,000-fold lower threshold dosages in the pe ...200111572015
a pear-derived kairomone with pheromonal potency that attracts male and female codling moth, cydia pomonella (l.).ethyl (2e, 4z)-2,4-decadienoate, a pear-derived volatile, is a species-specific, durable, and highly potent attractant to the codling moth (cm), cydia pomonella (l.), a serious pest of walnuts, apples, and pears worldwide. this kairomone attracts both cm males and virgin and mated females. it is highly attractive to cm in both walnut and apple orchard contexts, but has shown limited effectiveness in a pear orchard context. rubber septa lures loaded with ethyl (2e, 4z)-2,4-decadienoate remained a ...200111572014
a study of u.s. orchards to identify potential sources of escherichia coli o157:h7.the association of unpasteurized apple cider with escherichia coli o157:h7 foodborne illness has led to increased interest in potential reservoirs of this pathogen in the orchard. fourteen u.s. orchards were surveyed in autumn 1999 to determine the incidence and prevalence of e. coli o157:h7, e. coli, total aerobic microflora, and yeasts and molds. fruit samples (n = 63) (eight apple and two pear varieties) and soil, water, and fecal samples were collected. samples were plated on (i) tryptic soy ...200111563507
allergy to nonspecific lipid transfer proteins in rosaceae: a comparative study of different in vivo diagnostic methods.lipid transfer proteins (ltps) are the major allergens in patients sensitive to rosaceae (apple, peach, apricot, cherry, plum, and pear) who are not allergic to birch pollen.200111476467
live human germ cells in the context of their spermatogenic stages.various types of live, dispersed, human testicular cells in vitro were previously compared with the morphologic characteristics of human spermatogenic germ cells in situ within seminiferous tubules. the current study extends those observations by placing live human germ cells in the context of their developmental steps and stages of the spermatogenic cycle.200111473945
fingerprinting of polysaccharides attacked by hydroxyl radicals in vitro and in the cell walls of ripening pear fruit.hydroxyl radicals (*oh) may cause non-enzymic scission of polysaccharides in vivo, e.g. in plant cell walls and mammalian connective tissues. to provide a method for detecting the action of endogenous *oh in vivo, we investigated the products formed when polysaccharides were treated with *oh (generated in situ by ascorbate-h(2)o(2)-cu(2+) mixtures) followed by nab(3)h(4). treatment with *oh increased the number of nab(3)h(4)-reacting groups present in citrus pectin, homogalacturonan and tamarind ...200111463343
iron deficiency-associated changes in the composition of the leaf apoplastic fluid from field-grown pear (pyrus communis l.) trees.experiments have been carried out with field-grown pear trees to investigate the effect of iron chlorosis on the composition of the leaf apoplast. iron deficiency was associated with an increase in the leaf apoplastic ph from the control values of 5.5-5.9 to 6.5-6.6, as judged from direct ph measurements in apoplastic fluid obtained by centrifugation and fluorescence of leaves incubated with 5-cf. the major organic acids found in leaf apoplastic fluid of iron-deficient and iron-sufficient pear l ...200111457909
development and maturation of taste buds of the palatal epithelium of the rat: histological and immunohistochemical study.palatal taste buds are intriguing partners in the mediation of taste behavior and their spatial distribution is functionally important for suckling behavior, especially in the neonatal life. their prenatal development has not been previously elucidated in the rat, and the onset of their maturation remains rather controversial. we delineated the development and frequency distribution of the taste buds as well as the immunohistochemical expression of alpha-gustducin, a g protein closely related to ...200111455535
effects of tomato extract on human platelet aggregation in vitro.among all fruits tested in vitro for their anti-platelet property, tomato had the highest activity followed by grapefruit, melon, and strawberry, whereas pear and apple had little or no activity. tomato extract (20-50 microl of 100% juice) inhibited both adp- and collagen-induced aggregation by up to 70% but could not inhibit arachidonic acid-induced platelet aggregation and concomitant thromboxane synthesis under similar experimental conditions. the anti-platelet components (mw <1000 da) in tom ...200111454256
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