Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| a pseudoknot improves selection efficiency in ribosome display. | the size and diversity of ribosome display libraries depends upon stability of the complex formed between the ribosome, mrna and translated protein. to investigate if mrna secondary structure improves stability of the complex, we tested a pseudoknot, originating from the genomic rna of infectious bronchitis virus (ibv), a member of the positive-stranded coronavirus group. we used the previously-isolated anti-dna scfv, 3d8, as a target protein. during in vitro translation in rabbit reticulocyte l ... | 2007 | 17827535 |
| ultrastructural observations on effects of infectious bronchitis virus in eggshell-forming regions of the oviduct of the commercial laying hen. | the pathogenesis of 2 strains of infectious bronchitis virus (t and n1/88 strains) was studied in the eggshell-forming regions of the oviduct of commercial laying hens. there were no external shell deformities, except for paler shells. there was no decline in egg production in either of the infected groups. one hen from the n1/88-infected group and 2 hens from the t-infected group were out of lay. the light, scanning, and transmission electron microscopic changes in infected shell-forming region ... | 2007 | 17704379 |
| a massachusetts prototype like coronavirus isolated from wild peafowls is pathogenic to chickens. | coronavirus infection was investigated in apparently healthy wild peafowls in guangdong province of china in 2003, while severe acute respiratory syndrome (sars) broke out there. no sars-like coronavirus had been isolated but a novel avian coronavirus strain, peafowl/gd/kq6/2003 (kq6), was identified. sequence analysis revealed that kq6 was an avian coronavirus infectious bronchitis virus (ibv), a member of coronavirus in group 3. the genome sequence of kq6 had extremely high degree of identity ... | 2007 | 17629993 |
| intratracheally administered pathogen-associated molecular patterns affect antibody responses of poultry. | various potential immune-modulating microbially derived pathogen-associated molecular patterns (pamp), or so called homotopes, are present in high concentrations in the environment of food animals. in previous studies, intravenously administered pamp had variable effects on specific primary and secondary immune responses of poultry to systemically administered antigens. in the present study, we evaluated the effects of intratracheal (i.t.) challenge with the pamp lipopolysaccharide, lipoteichoic ... | 2007 | 17626812 |
| the efficacy of an avian metapneumovirus vaccine applied simultaneously with infectious bronchitis and newcastle disease virus vaccines to specific-pathogen-free chickens. | avian metapneumovirus (ampv), newcastle disease virus (ndv), and infectious bronchitis virus (ibv) are important respiratory pathogens of chickens. to achieve early posthatch protection against all three diseases it would be helpful to deliver live ampv, ibv, and ndv vaccines simultaneously at 1 day of age. however, previous work has indicated that the efficacy of ampv vaccines may be affected when codelivered with ibv or ndv vaccines. the efficacy of an ampv vaccine when codelivered to chickens ... | 2007 | 17626490 |
| evaluation of the effectiveness of two infectious bronchitis virus vaccine programs for preventing disease caused by a california ibv field isolate. | infectious bronchitis virus ca99 serotype was isolated from several broiler flocks in northern california. the virus caused late-onset respiratory disease and increased airsacculitis condemnation in affected flocks despite the use of an established infectious bronchitis virus vaccination program. an experimental study compared holland/arkansas and massachusetts/arkansas vaccination protocols to determine the efficacy of commercial infectious bronchitis virus vaccines in reducing respiratory dise ... | 2007 | 17626488 |
| molecular and serologic characterization, pathogenicity, and protection studies with infectious bronchitis virus field isolates from california. | in this study, we characterized three variant infectious bronchitis virus (ibv) strains isolated in 2003 and 2004 from broiler chickens in california and compared them to previously isolated california variant viruses and to common vaccine serotypes used in the united states. we conducted genetic, serologic, and pathogenicity studies on all three isolates, then tested different vaccines against one of the viruses. genetically the three variant ibv strains, designated ca557/03, ca706/03, and ca17 ... | 2007 | 17626478 |
| infection of hela cells by avian infectious bronchitis virus is dependent on cell status. | to investigate the adaptation of avian infectious bronchitis virus (ibv) in a human cell line may be beneficial to understanding the potential mechanisms of coronavirus interspecies infection. the current study addressed the poor replication of ibv in the hela human cell line demonstrated in previous reports. we showed that ibv strains m41, h52, h120 and gray could be propagated in hela cells with distinct cytopathic effect. the virus titre in freshly dispersed hela cells was 1000-fold higher th ... | 2007 | 17620171 |
| infectious bronchitis virus replication in the chicken embryo related cell line. | the susceptibility of the chicken embryo related (cer) cell line to infectious bronchitis virus (ibv m41) was characterized after five consecutive passages in cer cells. virus replication was monitored by cytopathic effect observation, electron microscopy, indirect immunofluorescence, and reverse transcription polymerase chain reaction (rt-pcr). at 96 h post-infection (p.i.), the cytopathic effect was graded 75% by cell fusion, rounding up of cells and monolayer detachment, and the electron micr ... | 2003 | 17585465 |
| detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in pakistan. | this study was conducted to investigate the incidence of infectious bronchitis virus (ibv) in commercial broiler and layer flocks in pakistan. serum samples from 16 layers and 9 broiler flocks were screened against m-41, d-274, d-1466, and 4-91 strain antigens using hemagglutination inhibition assay. overall, 88% of the flocks were seropositive for m-41 antibodies, whereas 40, 52, and 8% of the flocks were positive for d-274, d-1466, and 4-91 ibv strains, respectively. the m-41 antigen was also ... | 2007 | 17575179 |
| antiviral activity of carbohydrate-binding agents against nidovirales in cell culture. | coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like sars-cov, hku1 and nl63. together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order nidovirales. so far antivirals are hardly available to combat infections with viruses of this order. therefore, various antiviral strategies to counter nidoviral infections are under evaluation. lectins, which bind to n-linked oligosacch ... | 2007 | 17560666 |
| [construction of recombinant bcg bearing s1 glycoprotein of nephropathogenic ibv and study on its immunogenicity on chickens]. | glycoprotein si was the major protein to determine infection and immunogenicity of infectious bronchitis virus (ibv). the s1 glycoprotein gene of ibv isolates were amplified by reverse transcriptase-polymerase chain reaction (rt-pcr) and proved to be s1 gene by sequencing. the e. coli-mycobacterium expression shuttle plasmid pr-alpha-s1 was constructed by inserting the s1 gene to the prr3 with human mycobacterium tuberculosis hsp70 promoter and a signal peptide. then the plasmid pr-alpha-s1 was ... | 2007 | 17552243 |
| coronavirus associated with an enteric syndrome on a quail farm. | an enteric syndrome was observed in quail (coturnix coturnix) semi-intensively reared for restocking in apulia (southern italy). the birds showed depression, severe diarrhoea, dehydration and reduced growth. mortality occurred particularly in young birds. at necropsy the prominent lesion was enteritis. a coronavirus was detected by electron microscopy and reverse transcriptase-polymerase chain reaction in the faeces and in the intestinal content of the dead quails. the virus could not be cultiva ... | 2007 | 17497340 |
| s1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives. | infectious bronchitis virus ck/ch/ldl/97i was attenuated by serial passage in chicken embryos. virus of passage 115 was attenuated as determined by clinical response to inoculation to 15-day-old specific pathogen free chickens. the vaccination-challenge test showed that the attenuated passage 115 virus could afford protection against the homologous pathogenic virus, passage 5, by the clinical response. based on the sequence analysis and comparison of the s1 gene, both the pathogenic and attenuat ... | 2007 | 17497337 |
| cell cycle arrest and apoptosis induced by the coronavirus infectious bronchitis virus in the absence of p53. | manipulation of the cell cycle and induction of apoptosis are two common strategies used by many viruses to regulate their infection cycles. in cells infected with coronaviruses, cell cycle perturbation and apoptosis were observed in several reports. however, little is known about how these effects are brought out, and how manipulation of the functions of host cells would influence the replication cycle of coronavirus. in this study, we demonstrate that infection with coronavirus infectious bron ... | 2007 | 17493653 |
| lithium chloride inhibits the coronavirus infectious bronchitis virus in cell culture. | the avian coronavirus infectious bronchitis virus (ibv) is a major economic pathogen of domestic poultry that, despite vaccination, causes mortality and significant losses in production. during replication of the rna genome there is a high frequency of mutation and recombination, which has given rise to many strains of ibv and results in the potential for new and emerging strains. currently the live-attenuated vaccine gives poor cross-strain immunity. effective antiviral agents may therefore be ... | 2007 | 17479370 |
| heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus beaudette. | the avian coronavirus infectious bronchitis virus (ibv) strain beaudette is an embryo-adapted virus that has extended species tropism in cell culture. in order to understand the acquired tropism of the beaudette strain, we compared the s protein sequences of several ibv strains. the beaudette strain was found to contain a putative heparan sulfate (hs)-binding site, indicating that the beaudette virus may use hs as a selective receptor. to ascertain the requirements of cell-surface hs for beaudet ... | 2007 | 17461266 |
| different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in china. | chicken nephropathogenic infectious bronchitis (ib) was prevalent in the most chicken farms during recent years, although the ib vaccination program has been widely performed in china. to characterize the s1 protein of infectious bronchitis virus (ibv) from china, five representative nephropathogenic ib viruses isolated from chickens in different provinces were genetically and phylogenetically analyzed. the results showed that the length of the s1 genes of the isolates were quite different (1,61 ... | 2007 | 17431755 |
| the nephrosis-nephritis syndrome in chickens caused by a holte strain of infectious bronchitis virus. | 1969 | 17421918 | |
| manipulation of the infectious bronchitis coronavirus genome for vaccine development and analysis of the accessory proteins. | infectious bronchitis coronavirus (ibv) is the cause of the single most economically costly infectious disease of domestic fowl in the uk--and probably so in many countries that have a developed poultry industry. a major reason for its continued dominance is its existence as many serotypes, determined by the surface spike protein (s), cross-protection being poor. although controlled to some degree by live and inactivated vaccines, a new generation of ib vaccines is called for. reverse genetic or ... | 2007 | 17416443 |
| correlation between mechanical strength of messenger rna pseudoknots and ribosomal frameshifting. | programmed ribosomal frameshifting is often used by viral pathogens including hiv. slippery sequences present in some mrnas cause the ribosome to shift reading frame. the resulting protein is thus encoded by one reading frame upstream from the slippery sequence and by another reading frame downstream from the slippery sequence. although the mechanism is not well understood, frameshifting is known to be stimulated by an mrna structure such as a pseudoknot. here, we show that the efficiency of fra ... | 2007 | 17389398 |
| structure of the sars coronavirus nucleocapsid protein rna-binding dimerization domain suggests a mechanism for helical packaging of viral rna. | coronavirus nucleocapsid proteins are basic proteins that encapsulate viral genomic rna to form part of the virus structure. the nucleocapsid protein of sars-cov is highly antigenic and associated with several host-cell interactions. our previous studies using nuclear magnetic resonance revealed the domain organization of the sars-cov nucleocapsid protein. rna has been shown to bind to the n-terminal domain (ntd), although recently the c-terminal half of the protein has also been implicated in r ... | 2007 | 17379242 |
| sequence changes of infectious bronchitis virus isolates in the 3' 7.3 kb of the genome after attenuating passage in embryonated eggs. | attenuated infectious bronchitis virus (ibv) vaccines are available but the relationship between sequence and virulence is not clear. in this study, the sequences of the 3' 7.3 kb of the genome, amplified using reverse transcription-polymerase chain reaction before and after attenuation, were compared to study the relationship between virulence and the sequences of three ibv strains. after attenuation, two to six amino acid substitutions were found in the spike 1 subunit, and two or three amino ... | 2007 | 17364511 |
| characterisation of cyclin d1 down-regulation in coronavirus infected cells. | the positive strand rna coronavirus, infectious bronchitis virus (ibv), induces a g2/m phase arrest and reduction in the g1 and g1/s phase transition regulator cyclin d1. quantitative real-time rt-pcr and western blot analysis demonstrated that cyclin d1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. confocal microscopy revealed that cyclin d1 decreased in ibv-infected cells as infection progressed and inhibition studies i ... | 2007 | 17359980 |
| production and characterization of monoclonal antibodies to (poly100)s1 protein of avian infectious bronchitis virus. | fragments within s1 genes ((poly100)s1) of infectious bronchitis virus (ibv) strains zj971, m41 and sc021202 (sc) were subcloned into a prokaryotic expression vector and expressed in escherichia coli. monoclonal antibodies (mabs) against the recombinant (poly100)s1 proteins were produced, characterized and used to analyse epitopes on the s1 subunit of ibv. nine mabs raising from the three (poly100)s1 proteins recognized five different epitopes of the s1 subunit, designated as s1-a, b, c, d and e ... | 2007 | 17348910 |
| strong antiviral activity of heated and hydrated dolomite--preliminary investigation. | heated and hydrated naturally occurring dolomite showed very strong antiviral activity. infectivity of avian and human influenza, avian infectious bronchitis (coronavirus), newcastle disease (paramyxovirus) and avian laryngotracheitis (herpesvirus) viruses dropped at least 1,000 fold following contact with the dolomite for five minutes at 4 degrees c. dolomite is expected to be useful to inhibit the incidence of emerging and re-emerging infectious diseases. | 2007 | 17339770 |
| feline aminopeptidase n is not a functional receptor for avian infectious bronchitis virus. | coronaviruses are an important cause of infectious diseases in humans, including severe acute respiratory syndrome (sars), and have the continued potential for emergence from animal species. a major factor in the host range of a coronavirus is its receptor utilization on host cells. in many cases, coronavirus-receptor interactions are well understood. however, a notable exception is the receptor utilization by group 3 coronaviruses, including avian infectious bronchitis virus (ibv). feline amino ... | 2007 | 17324273 |
| ultrastructural study of infectious bronchitis virus infection in infundibulum and magnum of commercial laying hens. | the infundibulum and magnum of the oviduct were examined in hens in full lay which were infected with two australian strains of infectious bronchitis virus (ibv). the ultramicroscopic changes in the infundibulum and magnum were compared with control hens which had eggs at different positions in the oviduct. the ciliated and granular cells of the surface epithelia and secretory epithelial cells of the tubular glands were the target cells of ibv. no pathological changes were recorded during 2-8 da ... | 2007 | 17321700 |
| health evaluation of free-ranging and captive blue-fronted amazon parrots (amazona aestiva) in the gran chaco, bolivia. | bolivia has a total of 47 species of psittacidae, seven of which have been identified in our study site, the semiarid gran chaco of the isoso. one species, the blue-fronted parrot (amazona aestiva), is frequently captured by local isoseño guaraní indians for exploitation on the national and international market. these birds are often temporarily housed in small villages under unhygienic conditions with poultry and other domestic species. on occasion, these parrots escape back to the wild. additi ... | 2005 | 17312715 |
| coronavirus avian infectious bronchitis virus. | infectious bronchitis virus (ibv), the coronavirus of the chicken (gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. the virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney, oviduct and testes. it can be detected in both respiratory and faecal material. there is increasing ev ... | 2007 | 17296157 |
| isolation and identification of four infectious bronchitis virus strains in china and analyses of their s1 glycoprotein gene. | between 2003 and 2005, four strains of infectious bronchitis virus (ibv) were isolated from the vaccinated chicken flocks in china. the results from chicken embryo cross-neutralization assays showed that all the four isolates were relative to strain a2 of ibv, which was isolated in 1996 in beijing and related to strain 4/91. the s1 gene of the spike protein was amplified and sequenced. the nucleotide and amino acid sequence of the s1 gene had a similar degree of identity (88.98-99.28%) among the ... | 2007 | 17289305 |
| epididymal stone formation and decreased sperm production in roosters vaccinated with a killed strain of avian infectious bronchitis virus. | our objective was to determine if vaccination with killed avian infectious bronchitis virus (aibv) causes epididymal calcium stones in the rooster as is seen following vaccination with live attenuated aibv. specific-pathogen-free roosters were divided into three groups: nonvaccinated (nonvac), live attenuated aibv-vaccinated (lvac), and killed aibv-vaccinated (kvac) groups. roosters were vaccinated at 2, 6, 10, and 14 wk of age and the epididymal region was observed at 27 wk of age. epididymal s ... | 2006 | 17274299 |
| molecular analysis of brazilian infectious bronchitis field isolates by reverse transcription-polymerase chain reaction, restriction fragment length polymorphism, and partial sequencing of the n gene. | molecular analysis of 15 brazilian infectious bronchitis virus (ibv) isolates, obtained from clinical outbreaks of the disease in chickens (broilers or layers) in the state of minas gerais (brazil) between 1972 and 1989, is reported. using the n protein gene as target, ibvs were analyzed by reverse transcription-polymerase chain reaction/restriction fragment length polymorphism (rt-pcr/rflp) with the restriction enzymes avaii, hphi, sau96i, and tsp509i and cdna sequencing. results obtained from ... | 2006 | 17274284 |
| ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the n-terminal domain of n protein. | conserved among all coronaviruses are four structural proteins: the matrix (m), small envelope (e), and spike (s) proteins that are embedded in the viral membrane and the nucleocapsid phosphoprotein (n), which exists in a ribonucleoprotein complex in the lumen. the n-terminal domain of coronaviral n proteins (n-ntd) provides a scaffold for rna binding, while the c-terminal domain (n-ctd) mainly acts as oligomerization modules during assembly. the c terminus of the n protein anchors it to the vir ... | 2007 | 17229691 |
| characterization of the nuclear export signal in the coronavirus infectious bronchitis virus nucleocapsid protein. | the nucleocapsid (n) protein of infectious bronchitis virus (ibv) localizes to the cytoplasm and nucleolus and contains an eight-amino-acid nucleolar retention motif. in this study, a leucine-rich nuclear export signal (nes) (291-lqldglhl-298) present in the c-terminal region of the ibv n protein was analyzed by using alanine substitution and deletion mutagenesis to investigate the relative contributions that leucine residues make to nuclear export and where these residues are located on the str ... | 2007 | 17202223 |
| transcriptome of local innate and adaptive immunity during early phase of infectious bronchitis viral infection. | to understand the mechanistic basis of local innate and adaptive immunity against infectious bronchitis virus (ibv) at the molecular level, we examined the gene transcription profile of tracheal epithelial layers 3 d after infection of chickens with an attenuated ibv-massachusetts strain. results suggested that the transcription levels of 365 genes were either upregulated or downregulated (2-fold and higher) after ibv infection. among the upregulated 250 genes, 25 were directly immune-related ge ... | 2006 | 17201672 |
| preliminary crystallographic analysis of avian infectious bronchitis virus main protease. | infectious bronchitis virus (ibv) is the prototype of the genus coronavirus. it causes a highly contagious disease which affects the respiratory, reproductive, neurological and renal systems of chickens, resulting great economic losses in the poultry industry worldwide. the coronavirus (cov) main protease (m(pro)), which plays a pivotal role in viral gene expression and replication through a highly complex cascade involving the proteolytic processing of replicase polyproteins, is an attractive t ... | 2007 | 17183167 |
| histopathology of two serotypes of infectious bronchitis virus in laying hens vaccinated in the rearing phase. | the comparative histopathology of 2 different strains of infectious bronchitis virus (t and n1/ 88) in vaccinated hens was studied at 110 wk of age. the harderian gland showed similar histopathology in t- and n1/88-infected hens. the trachea and kidney of challenged vaccinated hens were protected to a moderate extent, but the oviduct was protected to only a small extent. the severity and persistence of lesions were greater in tubular shell gland, shell gland pouch, and kidney of the t-infected h ... | 2007 | 17179416 |
| comparative histopathology of two serotypes of infectious bronchitis virus (t and n1/88) in laying hens and cockerels. | the comparative and sequential histopathology of different tissues of unvaccinated laying hens and cockerels were studied in chickens exposed to t and n1/88 strain of infectious bronchitis virus (ibv). the harderian gland and trachea of hens and cockerels in both t- and n1/88-infected groups were damaged to a similar extent. the cecum was unaffected for both strains of ibv in both hens and cockerels. the sequential histopathological changes in hens revealed that ibv multiplies initially in the h ... | 2007 | 17179415 |
| infectious bronchitis virus serotypes in poultry flocks in jordan. | infectious bronchitis virus (ibv) causes respiratory disease in chickens all over the world. ibv has many serotypes that do not confer cross protection against each other. hemagglutination inhibition (hi) test has been used to determine the serotypes of ibv as a substitute to the more laborious virus neutralization test and the more sophisticated restriction endonuclease digestion or sequencing of the s1 gene. in jordan, no previous studies have been carried out to determine the involvement of i ... | 2007 | 17161477 |
| diseases of indigenous chickens in bokaa village, kgatleng district, botswana. | this study examined flock size and management, level of internal and external parasite burden and seroprevalence of antibodies to poultry pathogens in indigenous chickens in bokaa village, kgatleng district, botswana. the mean flock size was 22.6 +/- 6.85 with a range of 11-34. the mean body weights of cocks and hens were 2.28 +/- 0.56 kg and 1.70 +/- 0.38 kg, respectively. housing and commercial poultry feed were not provided. ascaridia galli, heterakis gallinarum and syngamus trachea were foun ... | 2006 | 17137053 |
| epidemiological and experimental evidence for immunodeficiency affecting avian infectious bronchitis. | we evaluated the effects of viral immunodeficiency on the outcome of infectious bronchitis virus (ibv) infection in chickens as a hypothetical cause for failure of adequate protection in vaccinated chickens. initially, we investigated ibv isolations from cases of respiratory disease in association with the presence of thymic and/or bursal atrophy in 322 submissions during 1997 to 2002. arkansas (ark)-type ibv was most frequently isolated in spite of extensive arkdpi vaccination in the broiler in ... | 2006 | 17121734 |
| capillary zone electrophoresis method for fingerprint of allantoic fluid in normal and infected spf embryonated chicken eggs. | a capillary zone electrophoresis method was developed for fingerprint determination of allantoic fluid in specific pathogen free (spf) embryonated chicken eggs. the effects of some crucial parameters, such as buffer type, ph, wavelength and running voltage on the separation were studied systematically. the components of the allantoic fluid were well separated using a fused-silica uncoated capillary with an effective length of 50 cm and an internal diameter of 50 microm. one hundred millimolars s ... | 2007 | 17055591 |
| characterisation of the rna binding properties of the coronavirus infectious bronchitis virus nucleocapsid protein amino-terminal region. | the coronavirus nucleocapsid (n) protein binds viral rna to form the ribonucleocapsid and regulate rna synthesis. the interaction of n protein with viral rna was investigated using circular dichroism and surface plasmon resonance. n protein underwent a conformational change upon binding viral rna and the data indicated electrostatic interactions were involved in the binding of the protein to rna. kinetic analysis suggested the amino-terminal region facilitates long-range non-specific interaction ... | 2006 | 17052713 |
| phylogenetic analysis of partial s1 and n gene sequences of infectious bronchitis virus isolates from italy revealed genetic diversity and recombination. | a total of ten infectious bronchitis virus (ibv) isolates collected from commercial chickens in italy in 1999 were characterized by rt-pcr and sequencing of the s1 and n genes. phylogenetic analysis based on partial s1 gene sequences showed that five field viruses clustered together with 793/b-type strains, having 91.3-98.5% nucleotide identity within the group, and one isolate had very close sequence relationship (94.6% identity) with 624/i strain. these two ibv types have been identified in it ... | 2007 | 17043759 |
| genes 3 and 5 of infectious bronchitis virus are accessory protein genes. | 2006 | 17037560 | |
| infectious bronchitis coronavirus induces cell-cycle perturbations. | 2006 | 17037559 | |
| three-dimensional reconstruction of the nucleolus using meta-confocal microscopy in cells expressing the coronavirus nucleoprotein. | 2006 | 17037551 | |
| avian infectious bronchitis virus enters cells via the endocytic pathway. | 2006 | 17037550 | |
| fluorescence dequenching assays of coronavirus fusion. | 2006 | 17037536 | |
| the transmembrane domain of the infectious bronchitis virus e protein is required for efficient virus release. | 2006 | 17037529 | |
| expression and structural analysis of infectious bronchitis virus nucleoprotein. | 2006 | 17037519 | |
| transcriptional regulation of rna3 of infectious bronchitis virus. | 2006 | 17037515 | |
| infectious bronchitis virus: s1 gene characteristics of vaccines used in china and efficacy of vaccination against heterologous strains from china. | the entire s1 protein genes of eight infectious bronchitis (ib) vaccine strains used in china were compared with those of the ib virus isolates present in the field in china. the nucleotide and amino acid similarities between the eight ib vaccine strains and the field strain, tl/ch/ldt3/03, which was isolated from a teal (anas sp.), were not more than 81.1% and 79.2%, respectively. phylogenetic analysis based on the s1 genes showed that the vaccines and field strains belonged to different cluste ... | 2006 | 16990149 |
| molecular epizootiology of avian infectious bronchitis in russia. | molecular characterization of infectious bronchitis viruses (ibvs) isolated between 1998 and 2002 from chickens in russia was performed. more than 250 field samples were tested by reverse transcriptase-polymerase chain reaction using two sets of primers corresponding to the most conserved 3'-untranslated region and the most variable s1 gene region of the viral genome. ninety-one ibv isolates were characterized by phylogenetic analysis of the s1 gene hypervariable region comprising 136 to 558 nuc ... | 2006 | 16990148 |
| influenza b viruses isolated in uruguay during the 2002-2005 seasons: genetic relations and vaccine strain match. | monitoring antigenic and genetic variations of circulating influenza viruses is critical for the selection of annual vaccine strains. in order to gain insight into the molecular evolution of influenza b viruses (ibv) isolated in uruguay in 2002 and 2005 outbreaks, antigenic and phylogenetic studies were carried out for the hemagglutinin (ha) gene. antigenic relations among uruguayan and reference strains isolated elsewhere were performed by means of hemagglutination inhibition assays (hai). gene ... | 2007 | 16987563 |
| s1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in egypt. | infectious bronchitis is highly contagious and constitutes one of the most common and difficult poultry diseases to control. ibv is endemic in probably all countries that raise chickens. it exists as dozens of serotypes/genotypes. only a few amino acid differences in the s1 protein of vaccine and challenge strains of ibv may result in poor protection. tropism of ibv includes the respiratory tract tissues, proventriculus and caecal tonsils of the alimentary tract, the oviduct and the kidney. | 2006 | 16987422 |
| an arginine-to-proline mutation in a domain with undefined functions within the helicase protein (nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells. | genetic manipulation of the rna genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of rna viruses. during construction of an infectious clone from a vero cell-adapted coronavirus infectious bronchitis virus (ibv), we found that a g-c point mutation at nucleotide position 15526, causing arg-to-pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of ibv on vero cells. when the in vitro-synthesized full-length tran ... | 2007 | 16979681 |
| effects of feeding grains naturally contaminated with fusarium mycotoxins on performance and metabolism of broiler breeders. | a study was conducted to investigate the effects of feeding grains naturally contaminated with fusarium mycotoxins on performance and metabolism of broiler breeders. forty-two 26-wk-old broiler breeder hens and nine 26-wk-old roosters were fed the following diets: (1) control, (2) contaminated grains, and (3) contaminated grains + 0.2% polymeric glucomannan mycotoxin adsorbent (gma) for 12 wk. the major contaminant was deoxynivalenol (12.6 mg/kg of feed), with lesser amounts of zearalenone and 1 ... | 2006 | 16977839 |
| amino acid residues critical for rna-binding in the n-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells. | the n-terminal domain of the coronavirus nucleocapsid (n) protein adopts a fold resembling a right hand with a flexible, positively charged beta-hairpin and a hydrophobic palm. this domain was shown to interact with the genomic rna for coronavirus infectious bronchitis virus (ibv) and severe acute respiratory syndrome coronavirus (sars-cov). based on its 3d structure, we used site-directed mutagenesis to identify residues essential for the rna-binding activity of the ibv n protein and viral infe ... | 2006 | 16971454 |
| comparison of sybr green i, elisa and conventional agarose gel-based pcr in the detection of infectious bursal disease virus. | the current available molecular method to detect infectious bursal disease virus (ibdv) is by reverse transcriptase-polymerase chain reaction (rt-pcr). however, the conventional pcr is time consuming, prone to error and less sensitive. in this study, the performances of sybr green i real-time pcr, enzyme-linked immunosorbent assay (elisa) and conventional agarose detection methods in detecting specific ibdv pcr products were compared. we found the real-time pcr was at least 10 times more sensiti ... | 2008 | 16971101 |
| isolation and characterization of a coronavirus from pigeons with pancreatitis. | to identify and partially characterize a coronaviruslike virus isolated from naturally infected pigeons. | 2006 | 16948604 |
| new genotype of infectious bronchitis virus in chickens in scotland. | 2006 | 16946315 | |
| sybr green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses. | coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. in this study, a one-step real-time reverse transcription-polymerase chain reaction (rt-pcr) assay based on sybr green chemistry and degenerate primers was developed for the generic detection of coronaviruses. the primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis v ... | 2007 | 16941059 |
| development and evaluation of a real-time taqman rt-pcr assay for the detection of infectious bronchitis virus from infected chickens. | it is important to rapidly differentiate infectious bronchitis virus (ibv) from disease agents like highly pathogenic avian influenza virus and exotic newcastle disease virus, which can be extremely similar in the early stages of their pathogenesis. in this study, we report the development and testing of a real-time rt-pcr assay using a taqman-labeled probe for early and rapid detection of ibv. the assay amplifies a 143-bp product in the 5'-utr of the ibv genome and has a limit of detection and ... | 2006 | 16934878 |
| genotypic and phenotypic characterization of the california 99 (cal99) variant of infectious bronchitis virus. | the california 99 (cal99) variant of infectious bronchitis virus (ibv) was first recovered in 1999 from vaccinated broiler chicken flocks in central california. the s1 hypervariable region of cal99 genome was most closely related to arkansas (ark) serotype viruses. in this study, the complete genome of cal99 was sequenced, and the structural protein genes were compared with those of commonly used ibv vaccines as well as those of isolates from naturally occurring outbreaks in different parts of t ... | 2007 | 16927130 |
| serological survey of the infectious disease status of old english game fowl in the lower north island, new zealand. | to investigate the serological status of old english game (oeg) cockerels for a range of infectious diseases of poultry. | 2006 | 16915341 |
| maternal antibody transfer from dams to their egg yolks, egg whites, and chicks in meat lines of chickens. | maternal antibodies are transferred from hens to the chicks via the egg. to gain insight into maternal antibody transfer and endogenous production of antibodies in broiler chicks, total igy, iga, igm, as well as anti-newcastle disease virus (ndv) and anti-infectious bronchitis (ibv) antibody levels were examined in the dams' plasma, egg yolks, egg whites, and chicks' plasma on d 3, 7, 14, and 21. blood was collected from 39-wk-old breeder hens (line 1, n = 17; line 2, n = 21). fertile eggs were ... | 2006 | 16903465 |
| detection of massachusetts and arkansas serotypes of infectious bronchitis virus in broilers. | the objective of this study was to compare the presence of the arkansas (ark) and massachusetts (mass) serotypes of infectious bronchitis virus (ibv) in the tracheas and cecal tonsils of commercial broilers after vaccination at 1 day of age by coarse spray. when given as a single serotype vaccine, the mass strain was detected by reverse transcriptase-polymerase chain reaction (rt-pcr)-restriction fragment length polymorphism (rflp) only in the tracheas, whereas the ark strain was detected in bot ... | 2006 | 16863085 |
| seroprevalence of avian influenza virus, infectious bronchitis virus, reovirus, avian pneumovirus, infectious laryngotracheitis virus, and avian leukosis virus in nigerian poultry. | eight poultry farms in nigeria, including chickens from nine breeder, 14 broiler, 28 pullet, 11 layer, and three cockerel flocks, were tested for antibody seroprevalence to the following poultry viruses of potential economic importance: infectious bronchitis virus (ibv), avian reovirus, avian pneumovirus (apv), infectious laryngotracheitis virus (iltv), avian influenza virus (aiv), and avian leukosis virus (alv). serum samples were collected between 1999 and 2004 and were tested for antibodies u ... | 2006 | 16863071 |
| safety and efficacy of an infectious bronchitis virus used for chicken embryo vaccination. | commercial vaccines for in ovo vaccination have not yet been developed for infectious bronchitis virus (ibv), the major coronavirus in the poultry industry. recombinant ibvs based on the beaudette strain expressing the beaudette spike protein (beau-r) or that from the virulent m41 strain (beaur-m41(s)) were assessed for their potential as prototype vaccines for application to 18-day-old embryos. pathogenicity was assessed by observing the effect on hatchability, and/or the production of nasal di ... | 2006 | 16860445 |
| changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus. | the nucleolus is a dynamic subnuclear structure involved in ribosome subunit biogenesis, cell cycle control and mediating responses to cell stress, among other functions. while many different viruses target proteins to the nucleolus and recruit nucleolar proteins to facilitate virus replication, the effect of infection on the nucleolus in terms of morphology and protein content is unknown. previously we have shown that the coronavirus nucleocapsid protein will localize to the nucleolus. in this ... | 2006 | 16819967 |
| hexamethylene amiloride blocks e protein ion channels and inhibits coronavirus replication. | all coronaviruses encode a small hydrophobic envelope (e) protein, which mediates viral assembly and morphogenesis by an unknown mechanism. we have previously shown that the e protein from severe acute respiratory syndrome coronavirus (sars-cov) forms cation-selective ion channels in planar lipid bilayers (wilson, l., mckinlay, c., gage, p., ewart, g., 2004. sars coronavirus e protein forms cation-selective ion channels. virology 330(1), 322-331). we now report that three other e proteins also f ... | 2006 | 16815524 |
| x-ray structures of the n- and c-terminal domains of a coronavirus nucleocapsid protein: implications for nucleocapsid formation. | coronaviruses cause a variety of respiratory and enteric diseases in animals and humans including severe acute respiratory syndrome. in these enveloped viruses, the filamentous nucleocapsid is formed by the association of nucleocapsid (n) protein with single-stranded viral rna. the n protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. we describe the structure of the two proteolytically resistant domains of the n p ... | 2006 | 16775348 |
| new variant of ibv in poland. | 2006 | 16766733 | |
| reciprocal antibody and complement responses of two chicken breeds to vaccine strains of newcastle disease virus, infectious bursal disease virus and infectious bronchitis virus. | serum antibody responses and haemolytic complement activity were evaluated in white leghorn (wlh) and rhode island red (rir) chickens that were vaccinated with live-attenuated vaccines of newcastle disease virus, or infectious bronchitis virus, or infectious bursal disease virus by means of ocular challenge at 10 times the normal vaccination dose. complement titres in non-vaccinated birds were significantly higher in wlh birds compared to rir birds. the lentogenic viral infection resulted in an ... | 2006 | 16755366 |
| delineation and modelling of a nucleolar retention signal in the coronavirus nucleocapsid protein. | unlike nuclear localization signals, there is no obvious consensus sequence for the targeting of proteins to the nucleolus. the nucleolus is a dynamic subnuclear structure which is crucial to the normal operation of the eukaryotic cell. studying nucleolar trafficking signals is problematic as many nucleolar retention signals (norss) are part of classical nuclear localization signals (nlss). in addition, there is no known consensus signal with which to inform a study. the avian infectious bronchi ... | 2006 | 16734668 |
| identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro. | purified avian infectious bronchitis virus (ibv) was used to screen a random phage display peptide library. after the fourth panning, 10 positive phages were sequenced and characterized. the phages specifically inhibited ibv infectivity in hela cells and blocked ibv haemagglutination. one linear peptide "gsh hrh vhs pfv" from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited ibv infection in hela as well. the results may contribute to developmen ... | 2006 | 16704119 |
| biochemical evidence for the presence of mixed membrane topologies of the severe acute respiratory syndrome coronavirus envelope protein expressed in mammalian cells. | coronavirus envelope (e) protein is a small integral membrane protein with multi-functions in virion assembly, morphogenesis and virus-host interaction. different coronavirus e proteins share striking similarities in biochemical properties and biological functions, but seem to adopt distinct membrane topology. in this report, we study the membrane topology of the sars-cov e protein by immunofluorescent staining of cells differentially permeabilized with detergents and proteinase k protection ass ... | 2006 | 16684538 |
| structure- and oil type-based efficacy of emulsion adjuvants. | oil-based emulsions are well-known immunopotentiators for inactivated, "killed" vaccines. we addressed the relationship between emulsion structure and levels of in vivo antibody formation to inactivated new castle disease virus (ndv) and infectious bronchitis virus (ibv) as antigens in 3-week-old chickens. the use of a polymeric emulsifier allowed for direct comparison of three types of emulsions, water-in-oil (w/o), oil-in-water (o/w) and w/o-in-water (w/o/w), while maintaining an identical con ... | 2006 | 16675072 |
| detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay. | the recombinant antigen obtained by cloning and expressing two ibv nucleocapsid protein fragments (143-414 aa, 281-414 aa) in escherichia coli was used for the detection of avian infectious bronchitis virus (ibv) specific antibodies in chicken sera by the indirect elisa (rnpibv-elisa). as a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rnpibv-elisa when comparing those of the routine whole ibv elisa have been shown to be 93.81% and 87.36%, respectively. the a ... | 2006 | 16675032 |
| determination of the effective dose of the live mycoplasma synoviae vaccine, vaxsafe ms (strain ms-h) by protection against experimental challenge. | the minimum effective dose of the mycoplasma synoviae-h (ms-h) vaccine was determined through protection against experimental challenge. chickens were vaccinated by eyedrop with the following doses of a vaccine: 1.2 x 10(5), 2.4 x 10(5) 4.8 x 10(5), 9.6 x 10(5), 1.92 x 10(6), and 3.84 x 10(6) color change units (ccu), then challenged 6 wk after vaccination. rapid serum agglutination results indicated that 100% of birds receiving an ms-h dose of > or = 4.8 x 10(5) ccu had antibodies to ms and enz ... | 2006 | 16617988 |
| sialic acid is a receptor determinant for infection of cells by avian infectious bronchitis virus. | the importance of sialic acid for infection by avian infectious bronchitis virus (ibv) has been analysed. neuraminidase treatment rendered vero, baby hamster kidney and primary chicken kidney cells resistant to infection by the ibv-beaudette strain. sialic acid-dependent infection was also observed with strain m41 of ibv, which infects primary chicken kidney cells but not cells from other species. in comparison with influenza a virus and sendai virus, ibv was most sensitive to pre-treatment of c ... | 2006 | 16603523 |
| infectious bronchitis virus s1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization. | five strains of infectious bronchitis virus isolated from commercial chickens from the state of pennsylvania, usa during the years 1998 and 1999 were studied. the strains were selected for cross-challenge in specific pathogen free chickens and virus neutralization in chick embryos on the basis of partial s1 sequence amino acid identity values. the partial sequences analysed spanned the hypervariable amino terminus region of s1 from amino acid residues 48 to 219, based on the beaudette strain. us ... | 2006 | 16595305 |
| isolation of a coronavirus from a green-cheeked amazon parrot (amazon viridigenalis cassin). | a virus (av71/99) was isolated from a green-cheeked amazon parrot by propagation and passage in both primary embryo liver cells derived from blue and yellow macaw (ara ararauna) embryos and chicken embryo liver cells. electron microscopic examination of cytopathic agents derived from both types of cell cultures suggested that it was a coronavirus. this was confirmed using a pan-coronavirus reverse transcriptase polymerase chain reaction that amplified part of gene 1 that encodes the rna-dependen ... | 2006 | 16595304 |
| antigenic and molecular characterization of isolates of the italy 02 infectious bronchitis virus genotype. | as part of an epidemiological surveillance of infectious bronchitis virus (ibv) in spain, four spanish field isolates showed high s1 spike sequence similarities with an ibv sequence from the genbank database named italy 02. given that little was known about this new emergent ibv strain we have characterized the four isolates by sequencing the entire s1 part of the spike protein gene and have compared them with many reference ibv serotypes. in addition, cross-virus neutralization assays were cond ... | 2006 | 16595297 |
| cell cycle perturbations induced by infection with the coronavirus infectious bronchitis virus and their effect on virus replication. | in eukaryotic cells, cell growth and division occur in a stepwise, orderly fashion described by a process known as the cell cycle. the relationship between positive-strand rna viruses and the cell cycle and the concomitant effects on virus replication are not clearly understood. we have shown that infection of asynchronously replicating and synchronized replicating cells with the avian coronavirus infectious bronchitis virus (ibv), a positive-strand rna virus, resulted in the accumulation of inf ... | 2006 | 16571830 |
| demonstration of an interference phenomenon associated with infectious bronchitis virus (ibv) of chickens. | 1949 | 16561757 | |
| evolutionary implications of avian infectious bronchitis virus (aibv) analysis. | for developing efficient vaccines, it is essential to identify which amino acid changes are most important to the survival of the virus. we investigate the amino acid substitution features in the avian infectious bronchitis virus (aibv) antigenic domain of a vaccine serotype (de072) and a virulent viral strain (ga98) to better understand adaptive evolution of aibv. in addition, the sars coronavirus (sars-cov) was also analyzed in the same way. it is interesting to find that extreme comparability ... | 2006 | 16541132 |
| the avian coronavirus infectious bronchitis virus undergoes direct low-ph-dependent fusion activation during entry into host cells. | coronaviruses are the causative agents of respiratory disease in humans and animals, including severe acute respiratory syndrome. fusion of coronaviruses is generally thought to occur at neutral ph, although there is also evidence for a role of acidic endosomes during entry of a variety of coronaviruses. therefore, the molecular basis of coronavirus fusion during entry into host cells remains incompletely defined. here, we examined coronavirus-cell fusion and entry employing the avian coronaviru ... | 2006 | 16537586 |
| coronaviruses in poultry and other birds. | the number of avian species in which coronaviruses have been detected has doubled in the past couple of years. while the coronaviruses in these species have all been in coronavirus group 3, as for the better known coronaviruses of the domestic fowl (infectious bronchitis virus [ibv], in gallus gallus), turkey (meleagris gallopavo) and pheasant (phasianus colchicus), there is experimental evidence to suggest that birds are not limited to infection with group 3 coronaviruses. in china coronaviruse ... | 2005 | 16537157 |
| evaluation of the health of free-ranging greater rheas (rhea americana) in argentina. | the health of 22 free-ranging adult rheas (rhea americana) examined and sampled during a translocation/reintroduction project and six juvenile rheas kept in semicaptivity was investigated, and details of their haematology and plasma biochemistry are presented. serological testing for antibodies to infectious agents was negative for infectious laryngotracheitis, avian adenovirus, avian influenza, avian reovirus, infectious bursal disease, infectious bronchitis virus, paramyxovirus types 1, 2, and ... | 2006 | 16517822 |
| [biological properties of the chick infectious bronchitis virus isolated in russia]. | a field chick infectious bronchitis virus (ibv) was isolated from the pathological material on chick embryos. the nucleotide sequence of the s1 gene was determined and comparatively analyzed with some sequences of this gene of foreign and russian vaccine strains and isolates. a cross-neutralization test using sera to various ibv seroptypes was performed. the isolate was shown to antigenically differ from the reference strains. bioassay was carried out, by using one-day chicks and the immunogenic ... | 2006 | 16515044 |
| detection and identification of avian, duck, and goose reoviruses by rt-pcr: goose and duck reoviruses are part of the same genogroup in the genus orthoreovirus. | a reverse transcription-polymerase chain reaction (rt-pcr) procedure for the detection of avian, duck, and goose reovirus (arv, drv, and grv) rna from cell culture supernatant and clinical samples was established. based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. the amplified, cloned, and sequenced 598-base-pair products from the sigmaa-encoding gene fragment from 16 isolates (ranging over 30 years) indicated that the primer regions were well conse ... | 2006 | 16502280 |
| isolation of a variant infectious bronchitis virus in australia that further illustrates diversity among emerging strains. | australian infectious bronchitis viruses (ibv) have undergone a separate evolution due to geographic isolation. consequently, changes occurring in australian ibv illustrate, independently from other countries, types of variability that could occur in emerging ibv strains. previously, we have identified two distinct genetic groups of ibv, designated subgroups 1 and 2. ibv strains of subgroup 1 have s1 and n proteins that share a high degree of amino acid identity, 81 to 98% in s1 and 91 to 99% in ... | 2006 | 16501892 |
| rapid differentiation of current infectious bronchitis virus vaccine strains and field isolates in australia. | rapid differentiation of vaccine strains of infectious bronchitis virus (ibv) from wild type strains would enhance investigations of disease outbreaks. this study aimed to develop a reverse transcription-polymerase chain reaction (rt-pcr) assay to differentiate between australian vaccine strains of ibv and field isolates. | 2006 | 16498838 |
| comparison of 3'-end encoding regions of turkey coronavirus isolates from indiana, north carolina, and minnesota with chicken infectious bronchitis coronavirus strains. | to analyze the 3'-end structural protein-encoding region of turkey coronavirus (tcov) isolates associated with outbreaks of acute enteritis in indiana, north carolina, or minnesota. | 2006 | 16491018 |
| oral immunoadjuvant activity of lactobacillus casei subsp. casei in dextran-fed layer chickens. | we recently reported that synbiotic lactobacillus casei subsp. casei together with specific substrate dextran elicited an enhancement in humoral immune response against bovine serum albumin (bsa) as a model antigen in balb/c mice. the present study was designed to evaluate the oral immunoadjuvant effects of the synbiotic in layer chickens. using a pcr assay, l. casei subsp. casei was detected specifically in the intestinal chyme of chickens (10 d of age, julia strain) fed ad libitum on a diet su ... | 2006 | 16469163 |
| typing infectious bronchitis virus strains using reverse transcription-polymerase chain reaction and restriction fragment length polymorphism analysis to compare the 3' 7.5 kb of their genomes. | typing infectious bronchitis virus (ibv) strains is useful for implementation of control measures and for understanding the epidemiology and evolution of ibv. the aim of the work reported here was to develop a rapid and sensitive method for typing isolates of ibv, if possible directly from tissues of infected birds. a procedure was developed for differentiation of ibv strains by restriction endonuclease fragment length polymorphism (rflp) analysis of a 7.5 kb dna fragment amplified from their ge ... | 2006 | 16448945 |
| murine encephalitis caused by hcov-oc43, a human coronavirus with broad species specificity, is partly immune-mediated. | the human coronavirus hcov-oc43 causes a significant fraction of upper respiratory tract infections. most coronaviruses show a strong species specificity, although the sars-coronavirus crossed species from palm civet cats to infect humans. similarly, hcov-oc43, likely a member of the same coronavirus group as sars-cov, readily crossed the species barrier as evidenced by its rapid adaptation to the murine brain [mcintosh, k., becker, w.b., chanock, r.m., 1967. growth in suckling-mouse brain of "i ... | 2006 | 16413043 |