Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| malate dehydrogenase is negatively regulated by mir-1 in trichomonas vaginalis. | micrornas are highly conserved small noncoding rnas that can suppress protein translation through complementary binding to target mrnas. we used a novel approach to identify mirna targets in the protist trichomonas vaginalis by comparing the levels of differentially expressed proteins and genes in the trophozoite and amoeboid stages. we observed that the t. vaginalis malate dehydrogenase (tv_mdh) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold. b ... | 2009 | 19777264 |
| axenization and optimization of in vitro growth of clonal cultures of tetratrichomonas gallinarum and trichomonas gallinae. | a rapid and simple procedure was established to obtain clonal axenic cultures of tetratrichomonas gallinarum and trichomonas gallinae and to optimize their in vitro growth conditions. medium 199 was used for axenization of two genetically different clones of t. gallinarum and t. gallinae. six different media were used to optimize the growth behaviour of axenically grown parasites: medium 199, tym, tyi-s-33, hollander fluid (hf), trichomonas vaginalis (tv) and modified tv media. the highest cell ... | 2010 | 19766633 |
| the mercaptopyruvate sulfurtransferase of trichomonas vaginalis links cysteine catabolism to the production of thioredoxin persulfide. | trichomonas vaginalis is a protozoan parasite of humans that is able to synthesize cysteine de novo using cysteine synthase but does not produce glutathione. in this study, high pressure liquid chromatography analysis confirmed that cysteine is the major intracellular redox buffer by showing that t. vaginalis contains high levels of cysteine ( approximately 600 mum) comprising more than 70% of the total thiols detected. to investigate possible mechanisms for the regulation of cysteine levels in ... | 2009 | 19762467 |
| the evolution of an integrated sexual health service. | the objective of the study was to assess the contributions of different providers across an integrated sexual health-care network to the diagnosis of sexually transmitted infections (stis). the method employed was retrospective cross-sectional survey of laboratory tests for chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis between 01 april 2004 and 31 march 2007. the total number of sti screens in all venues rose from 17,126 to 23,679. the proportion of sti screens occurring ... | 2009 | 19759047 |
| steroid hormones alter amp hydrolysis in intact trophozoites of trichomonas vaginalis. | trichomonas vaginalis infection may be influenced by the vaginal concentrations of estrogens. we have investigated the effects of 17beta-estradiol and dehydroepiandrosterone sulfate (dheas) on the ecto-5'-nucleotidase activity in fresh clinical (vp60) and in long-term-grown (30236 atcc) isolates of t. vaginalis. in vitro exposure to dheas and 17beta-estradiol did not induce any changes in adenosine monophosphate (amp) hydrolysis in these isolates. the treatment of parasites in the presence of dh ... | 2009 | 19756747 |
| synthesis and biological evaluation of new heterocyclic quinolinones as anti-parasite and anti-hiv drug candidates. | we have synthesized quinolinones with potential antiparasitic and anti-hiv activities by an original two-step method involving microwave irradiation and have evaluated their activities against plasmodium falciparum, leishmania donovani, trichomonas vaginalis, and hiv. none of the tested compounds had been previously described using this method of synthesis. one of the compounds had interesting antiparasitic and anti-hiv activity, which could be improved by substitution with different radicals. | 2009 | 19748781 |
| genomic organization and promoter analysis of the trichomonas vaginalis core histone gene families. | core histone gene is a well-established model to study eukaryote gene transcription regulation mechanism. however, the protozoan core histone gene regulation mechanism remains largely unknown. in this study, we observed almost all protozoan trichomonas vaginalis core histone genes (60/74) organize as gene pairs in a head-to-head manner, thus facilitating the divergent transcription of both partners. additionally, the majority of both t. vaginalis core histone genes pairs (50/60) and solitary gen ... | 2010 | 19744576 |
| prospective study of trichomonas vaginalis infection and prostate cancer incidence and mortality: physicians' health study. | a recent nested case-control study found that the presence of antibodies against trichomonas vaginalis, a common nonviral sexually transmitted infection, was positively associated with subsequent incidence of prostate cancer. we confirmed these findings in an independent population and related serostatus for antibodies against t vaginalis to prostate cancer incidence and mortality. | 2009 | 19741211 |
| an iatrogenic confounding variable. | 2009 | 19741210 | |
| risk factors for sexually transmitted infections in women in rural northeast brazil. | sexually transmitted infections (stis) are highly prevalent in northeast brazil, but factors associated with the presence of an sti have rarely been studied systematically. | 2008 | 19738353 |
| trichomoniasis and other sexually transmitted infections: results from the 2001-2004 national health and nutrition examination surveys. | to estimate the association between trichomonas vaginalis infection (tv) and 6 sexually transmitted infections: chlamydia, gonorrhea, herpes simplex virus (types 1 and 2), syphilis, and human immunodeficiency virus (hiv) in a nationally representative sample. | 2009 | 19734826 |
| proinflammatory cytokine and nitric oxide production by human macrophages stimulated with trichomonas vaginalis. | trichomonas vaginalis commonly causes vaginitis and perhaps cervicitis in women and urethritis in men and women. macrophages are important immune cells in response to t. vaginalis infection. in this study, we investigated whether human macrophages could be involved in inflammation induced by t. vaginalis. human monocyte-derived macrophages (hmdm) were co-cultured with t. vaginalis. live, opsonized-live trichomonads, and t. vaginalis lysates increased proinflammatory cytokines, such as tnf-alpha, ... | 2009 | 19724692 |
| trichomonas vaginalis kills and eats--evidence for phagocytic activity as a cytopathic effect. | this study reports that the cytopathic effect of trichomonas vaginalis, an important human parasite of the urogenital tract, occurs due to mechanical stress and subsequent phagocytosis of the necrotic cells. the investigation was done using a primary culture of bovine oviduct epithelial cells (boecs), grown either in monolayers or as floating cells. trophozoites displaying different virulence levels were co-incubated with boecs for times varying between 1 min and 48 h. analyses were performed us ... | 2010 | 19723359 |
| mycoplasma genitalium as a contributor to the multiple etiologies of cervicitis in women attending sexually transmitted disease clinics. | the purpose of this study was to investigate the prevalence of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and mycoplasma genitalium, in women attending a sexually transmitted disease (std) clinic, as well as the frequency of coinfections, and relationship of each organism to cervicitis. | 2009 | 19704398 |
| correlates of sexually transmitted infection histories in a cohort of american male health professionals. | several epidemiologic studies have investigated sexually transmitted infections (stis) and later risk of genitourinary conditions with suggestive positive results. while these results may reflect causal associations, other possible explanations include confounding by factors possibly related to both sti acquisition and genitourinary condition risk such as recognized sti-risk factors/correlates, and other factors not typically considered in relation to stis (e.g., general health-related behaviors ... | 2009 | 19655261 |
| physical health consequences of sexual assault victims. | to describe health consequences of sexual assault victims attending police general hospital, thailand. | 2009 | 19626805 |
| the protist trichomonas vaginalis harbors multiple lineages of transcriptionally active mutator-like elements. | for three decades the mutator system was thought to be exclusive of plants, until the first homolog representatives were characterized in fungi and in early-diverging amoebas earlier in this decade. | 2009 | 19622157 |
| a quantitative resazurin assay to determinate the viability of trichomonas vaginalis and the cytotoxicity of organic solvents and surfactant agents. | trichomonas vaginalis causes trichomonosis, the most common, non-viral sexually transmitted disease. to test anti-trichomonas agents, usually many with low water solubility, organic solvents and surfactant agents should be used. therefore, the minimal inhibitory concentration (mic) of acetone, methanol, ethanol, isopropanol, dmso, tween 20, tween 80, and triton x-100 was determined against t. vaginalis isolates using the quantitative resazurin method. our results showed that solvents and surfact ... | 2009 | 19619538 |
| trichomonas vaginalis infection and associated risk factors in a socially-marginalized female population in coastal peru. | the epidemiology of trichomonas vaginalis infection among sexually active socially-marginalized women in three urban, coastal peruvian cities was examined in order to quantify the prevalence of trichomonas infection and identify associated risk factors. | 2009 | 19584943 |
| depression, sexually transmitted infection, and sexual risk behavior among young adults in the united states. | to measure associations among depression, sexual risk behaviors, and sexually transmitted infection (sti) among white and black youth in the united states. | 2009 | 19581548 |
| diagnosis of trichomonas vaginalis in female children and adolescents evaluated for possible sexual abuse: a comparison of the inpouch tv culture method and wet mount microscopy. | to compare the performance of wet mount microscopy and the inpouch tv culture method in diagnosing trichomonas vaginalis in a cohort of female children and adolescents evaluated for possible sexual abuse. | 2009 | 19576816 |
| epidemiology of sexually transmitted infections in suspected child victims of sexual assault. | the objective of this study was to describe the epidemiology of neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, treponema pallidum, hiv, and herpes simplex virus type 2 (hsv-2) infection diagnosed by culture or by serologic or microscopic tests and by nucleic acid amplification tests in children who are evaluated for sexual victimization. | 2009 | 19564286 |
| vaginal and endocervical microorganisms in symptomatic and asymptomatic non-pregnant females: risk factors and rates of occurrence. | physiological or non-physiological factors may affect the vaginal flora. the occurrence of genital microorganisms in non-pregnant females of all ages was studied, as were the risk factors associated with each microorganism. a retrospective analysis of vaginal and endocervical cultures and wet smears from 27,172 non-pregnant women, between 1996 to 2005, was performed taking into consideration clinical and socio-demographic characteristics. no microorganisms were observed in 55.7% of the individua ... | 2009 | 19558525 |
| the association between oral contraceptives, depot-medroxyprogesterone acetate, and trichomoniasis. | hormonal contraception use by women may increase the risk of acquiring certain sexually transmitted infections. we explored the effect of hormonal contraceptive use, specifically oral contraception (oc), and depot-medroxyprogesterone acetate (dmpa) on trichomonas vaginalis infections in women. | 2009 | 19556926 |
| redox properties of the oxygen-detoxifying flavodiiron protein from the human parasite giardia intestinalis. | flavodiiron proteins (fdps) are enzymes identified in prokaryotes and a few pathogenic protozoa, which protect microorganisms by reducing o(2) to h(2)o and/or no to n(2)o. unlike most prokaryotic fdps, the protozoan enzymes from the human pathogens giardia intestinalis and trichomonas vaginalis are selective towards o(2). uv/vis and epr spectroscopy showed that, differently from the no-consuming bacterial fdps, the giardia fdp contains an fmn with reduction potentials for the formation of the si ... | 2009 | 19545535 |
| adult male circumcision does not reduce the risk of incident neisseria gonorrhoeae, chlamydia trachomatis, or trichomonas vaginalis infection: results from a randomized, controlled trial in kenya. | we examined the effect of male circumcision on the acquisition of 3 nonulcerative sexually transmitted infections (stis). | 2009 | 19545209 |
| [tinidazole: a classical anaerobical drug with multiple potential uses nowadays]. | tinidazole is a 5-nitroimidazole active in vitro against a wide variety of anaerobic bacteria and protozoa. tinidazole is an effective treatment against anaerobic microorganisms based on its pharmacokinetic characteristics (c(max) 51 microg/ml, t(1/2) 12.5 h) and its excellent in vitro activity. its long half-life allows once a day regimens. tinidazole is as effective as metronidazole in the treatment of infections caused by t. vaginalis, giardiasis and amebiasis and bacterial vaginosis, malaria ... | 2009 | 19544102 |
| responsiveness of trichomonas vaginalis to iron concentrations: evidence for a post-transcriptional iron regulation by an ire/irp-like system. | trichomonas vaginalis has high iron-dependency, favoring its growth and multiplication in culture. iron also regulates some of the trichomonal virulence properties by yet unknown mechanisms. iron is an essential but potentially toxic metal for the majority of organisms. thus, its concentration must be tightly regulated within the cell. in mammals, the iron homeostasis is mainly regulated at the post-transcriptional level by a well known mechanism mediated by the binding of iron regulatory protei ... | 2009 | 19539055 |
| efficacy of two plant extracts against vaginal trichomoniasis. | trichomoniasis vaginalis is now an important worldwide health problem. metronidazole has so far been used in treatment, but the metronidazole-resistant strains and unpleasant adverse effects have been developed. treatment of patients with metronidazole refractory vaginal trichomoniasis constitutes a major therapeutic challenge and treatment options are extremely limited. in the present study, 33 metronidazole-resistant t. vaginalis females were treated with a combined course of metronidazole and ... | 2009 | 19530609 |
| polymerase chain reaction versus conventional methods in the diagnosis of vaginal trichomoniasis. | a total of 200 females of whom 120 had manifestations of vaginal trichomoniasis and 80 asymptomatic ones were studied. in 54/120 symptomatic female (45%) and in 28/80 asymptomatic ones (35%), t. vaginalis was diagnosed by wet mount of bedside vaginal swab samples. of 120 samples from symptomatic females, t. vaginalis was detected in 93 (77.5%) when cultured onto inpouch & 95 (79.16%) in modified thioglycolate media. culturing 80 samples of asymptomatic females showed t. vaginalis in 35 (43.75%) ... | 2009 | 19530606 |
| [leucoplakia vesicae in females: diagnosis and treatment]. | sixty patients suffering from leucoplakia vesicae (lv) were examined using cystoscopy with biopsy of the urinary bladder wall, blood enzyme immunoassay for detection of antibodies to agents of sexually transmitted infections (sti), uroflowmetry, culture analysis of cervical canal and mucosa samples for sti. as shown by a pathomorphological examination of the vesical mucosa biopsy specimens, long-term persistence of pathogenic (chlamydia, trichomonades) and opportunistic (mycoplasma, ureaplasma, ... | 2009 | 19526870 |
| a study comparing sexually transmitted infections and hiv among ex-red-light district and non-red-light district sex workers after the demolition of baina red-light district. | in june 2004, the red-light area of goa was demolished. we compare hiv and sexually transmitted infections (stis) between sex workers who had been based in baina red-light district (ex-rld-sws) with sex workers who had never worked in baina (non-rld-sws). | 2009 | 19525855 |
| trichomoniasis in teens: an update. | trichomoniasis, caused by the protozoa trichomonas vaginalis, is one of the oldest sexually transmitted infections. since the advent of more accurate diagnostic tests, the epidemiology and consequences of infection with t. vaginalis can be described more precisely. this review will highlight new diagnostic methods, the epidemiology of trichomoniasis, and discuss the merits of improved screening for this pathogen in adolescent women. | 2009 | 19491679 |
| bacterial vaginosis in female facility workers in north-western tanzania: prevalence and risk factors. | to determine prevalence of, and risk factors for, bacterial vaginosis (bv) among herpes simplex virus (hsv) 2 seropositive tanzanian women at enrollment into a randomised, placebo-controlled trial of hsv suppressive treatment. | 2009 | 19473997 |
| in vitro activities of three synthetic peptides derived from epinecidin-1 and an anti-lipopolysaccharide factor against propionibacterium acnes, candida albicans, and trichomonas vaginalis. | the synthetic epinecidin-1(22-42) peptide was derived from positions 22-42 of epinephelus coioides epinecidin-1. the synthetic salf(55-76) cyclic peptide (cssalf(55-76)) and salf(55-76) linear peptide (lssalf(55-76)) contained sequences from positions 55 to 76 of the penaeus monodon anti-lipopolysaccharide factor (alf), respectively. we studied the in vitro activities of epinecidin-1(22-42), cssalf(55-76), and lssalf(55-76) against propionibacterium acnes, candida albicans, and trichomonas vagin ... | 2009 | 19463737 |
| anal sex is a behavioural marker for laboratory-confirmed vaginal sexually transmissible infections and hiv-associated risk among african-american female adolescents. | african-american females are disproportionately affected by hiv and sexually transmissible infections (stis). the prevalence of anal sex and its association with other sexual risk behaviours is understudied in this population. | 2009 | 19457289 |
| examination for double-stranded rna viruses in trichomonas gallinae and identification of a novel sequence of a trichomonas vaginalis virus. | to determine if double-stranded rna (dsrna) viruses exist and are potential virulence factors in trichomonas gallinae, virus purification via ultracentrifugation was attempted for 12 t. gallinae isolates recovered from wild birds. following purification, virus-like particles were not observed by transmission electron microscopy, nor were dsrna segments visualized in agarose gels after electrophoresis of extracted rna from any of the 12 t. gallinae isolates. however, virus particles and dsrna seg ... | 2009 | 19452169 |
| identification of microrna in the protist trichomonas vaginalis. | micrornas (mirnas) are a class of small noncoding rnas that have important regulatory roles in multicellular organisms. however, mirna has never been identified experimentally in protist. direct cloning of 438 expressed mirna tags by microrna serial analysis of gene expression from the parasitic protist trichomonas vaginalis identified nine candidate mirnas. bioinformatics analysis of the corresponding genomic region revealed that these mirna candidates contain a classical stem-loop-stem structu ... | 2009 | 19442639 |
| disentangling contributions of reproductive tract infections to hiv acquisition in african women. | : to estimate the effects of reproductive tract infections (rtis) on hiv acquisition among zimbabwean and ugandan women. | 2009 | 19434010 |
| comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome. | we examined a total of 194 patients over 18 years of age with chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities. the following data were obtained for each patient: clinical history--the severity of chronic prostatitis symptoms scored by a croatian translation of the nih cpsi questionnaire, clinical status including digitorectal examination, urethral swab specimens, and selective samples of urine and expressed prostatic secretion, acc ... | 2009 | 19423471 |
| [steroidal saponins from the plant agave brittoniana with activity against the parasite trichomona vaginalis]. | the genus agave (agavaceae), includes more than 300 species; around 16 of them show an homogeneous distribution throughout cuba. agave brittoniana (ssp. brachypus), is an endemic subspecies that grows in the central region of the country and its leaves are traditionally used in the treatment of parasitic diseases. the parasite trichomonas vaginalis causes the disease known as trichomoniasis, that infects the genital tract. to test in vitro the plant against trichomona vaginalis, the dried and po ... | 2008 | 19419073 |
| trichomonas vaginalis: metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. implications for nitroimidazole toxicity and resistance. | infections with the microaerophilic parasite trichomonas vaginalis are treated with the 5-nitroimidazole drug metronidazole, which is also in use against entamoeba histolytica, giardia intestinalis and microaerophilic/anaerobic bacteria. here we report that in t. vaginalis the flavin enzyme thioredoxin reductase displays nitroreductase activity with nitroimidazoles, including metronidazole, and with the nitrofuran drug furazolidone. reactive metabolites of metronidazole and other nitroimidazoles ... | 2009 | 19415801 |
| use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. | to evaluate a new dual priming oligonucleotide (dpo)-based multiplex polymerase chain reaction (pcr) assay for detection of six sexually transmitted pathogens, including chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum and trichomonas vaginalis. | 2009 | 19413770 |
| characteristics of hiv-1 discordant couples enrolled in a trial of hsv-2 suppression to reduce hiv-1 transmission: the partners study. | the partners hsv-2/hiv-1 transmission study (partners study) is a phase iii, placebo-controlled trial of daily acyclovir for genital herpes (hsv-2) suppression among hiv-1/hsv-2 co-infected persons to reduce hiv-1 transmission to their hiv-1 susceptible partners, which requires recruitment of hiv-1 serodiscordant heterosexual couples. we describe the baseline characteristics of this cohort. | 2009 | 19404392 |
| alfred francois donné (1801-78): a pioneer of microscopy, microbiology and haematology. | alfred françois donné is widely known in the scientific community as the discoverer of trichomonas vaginalis, since he was the first to illustrate the parasite that later was recognized to cause vaginal infections. however, his other, less-known findings are equally important: he was also the inventor of the photoelectric microscope, with the assistance of his student léon foucault, as well as the first to apply photography to microscopic preparations (daguerreotype). his research in microscopy ... | 2009 | 19401511 |
| simple and inexpensive point-of-care tests improve diagnosis of vaginal infections in resource constrained settings. | bacterial vaginosis (bv) and trichomonas vaginalis infection (tv) have been associated with adverse birth outcomes and increased risk for hiv. we compare the performance of simple inexpensive point-of-care (poc) tests to laboratory diagnosis and syndromic management of bv and tv in poor settings. | 2009 | 19392745 |
| three-dimensional structure of the cytoskeleton in trichomonas vaginalis revealed new features. | the flagellated protozoan trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. the complicated three-dimensional (3d) structure of the cellular components of t. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. electron tomography was used to examine the detailed structure of the cellular organelles. tomographic reconstruction showed the mastigont system and the parabasal filament of ... | 2009 | 19386993 |
| high rates of std and sexual risk behaviors among garífunas in honduras. | honduras has the highest concentration of hiv and aids cases in central america, with an estimated adult hiv prevalence of 1.5%. prevalence is higher among certain ethnic groups such as the garífuna with a reported hiv prevalence of 8%. | 2009 | 19384098 |
| mycoplasma genitalium compared to chlamydia, gonorrhoea and trichomonas as an aetiological agent of urethritis in men attending std clinics. | to investigate prevalence of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis in men, frequency of co-infections, and association of organisms with urethritis in men. | 2009 | 19383597 |
| glyceraldehyde-3-phosphate dehydrogenase is a surface-associated, fibronectin-binding protein of trichomonas vaginalis. | trichomonas vaginalis colonizes the urogenital tract of humans and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. we have shown an association of t. vaginalis with basement membrane extracellular matrix components, a property which we hypothesize is important for colonization and persistence. in this study, we identify a fibronectin (fn)-binding protein of t. vaginalis. a monoclonal antibody (mab) from a library of hybridomas that inhibited the binding of t. vagi ... | 2009 | 19380472 |
| simultaneous identification of 14 genital microorganisms in urine by use of a multiplex pcr-based reverse line blot assay. | the aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. a multiplex pcr-based reverse line blot (mpcr/rlb) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely trichomonas vaginalis, streptococcus pneumoniae, neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma parvum, u. urealyticum, gardnerella vaginalis, haemophilus influenzae, herpes simplex virus type 1 (hsv1) and hsv2, ... | 2009 | 19357202 |
| association between group a beta-haemolytic streptococci and vulvovaginitis in adult women: a case-control study. | guidelines for the management of vaginal discharge mention candida albicans, trichomonas vaginalis, bacterial vaginosis, chlamydia trachomatis and neisseria gonorrhoeae as causes and do not recommend full microbiological culture. the role of non-group b beta-haemolytic streptococci in vaginal cultures is unclear, except for group a streptococci that are known to cause vulvovaginitis in children. in a case-control study, we investigated the association between non-group b beta-haemolytic streptoc ... | 2009 | 19343383 |
| clinical significance of trichomonas vaginalis detected in papanicolaou smear: a survey in female social hygiene clinic. | to evaluate the clinical significance of trichomonas vaginalis detected in papanicolaou (pap) smears in our local population. | 2009 | 19342733 |
| the microaerophilic flagellate, trichomonas vaginalis, contains unusual acyl lipids but no detectable cardiolipin. | previous lipid analysis of trichomonads has led to controversy as to whether these hydrogenosome-containing organisms contain cardiolipin (cl), which is a characteristic component of mitochondria. here we report a careful lipid analysis of the sexually transmitted protist trichomonas vaginalis. major lipids were phosphatidylethanolamine (42%) and phosphatidylcholine (20%) with lesser amounts of phosphatidylglycerol (pg) (12%) and non-polar components. two unusual lipids, acyl-pg (8%) and ceramid ... | 2009 | 19335774 |
| sexually transmitted infections and reproductive tract infections in female sex workers. | the present study was conducted in 300 female sex workers (fsws) from surat city in 2005-2006. vaginal swabs, endocervical swabs and serum samples were collected from each of these fsws. vaginal samples were screened for bacterial vaginosis (bv), candidiasis and trichomonas vaginalis . endocervical swabs were screened for gonococcal infection. serological tests for human immunodeficiency virus (hiv), hepatitis b surface antigen (hbsag) and syphilis were performed. from a total of 300 fsws, bv wa ... | 2009 | 19332911 |
| prospective study of correlates of vaginal lactobacillus colonisation among high-risk hiv-1 seronegative women. | vaginal colonisation with lactobacillus species is characteristic of normal vaginal ecology. the absence of vaginal lactobacilli, particularly hydrogen peroxide (h(2)o(2))-producing isolates, has been associated with symptomatic bacterial vaginosis (bv) and increased risk for hiv-1 acquisition. identification of factors associated with vaginal lactobacillus colonisation may suggest interventions to improve vaginal health. | 2009 | 19329442 |
| genetic identity and differential gene expression between trichomonas vaginalis and trichomonas tenax. | trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (std) worldwide, while t. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. the extent of genetic identity between t. vaginalis and its oral commensal counterpart is unknown. | 2009 | 19296850 |
| comparative analyses among the trichomonas vaginalis, trichomonas tenax, and tritrichomonas foetus 5s ribosomal rna genes. | the 5s ribosomal rna (5s rrna) is an essential component of ribosomes. throughout evolution, variation is found among 5s rrna genes regarding their chromosomal localization, copy number, and intergenic regions. in this report, we describe and compare the gene sequences, motifs, genomic copy number, and chromosomal localization of the trichomonas vaginalis, trichomonas tenax, and tritrichomonas foetus 5s rrna genes. t. vaginalis and t. foetus have a single type of 5s rrna-coding region, whereas t ... | 2009 | 19290527 |
| validity of the symptomatic approach used by nurses in diagnosing vaginal infections. | to examine the validity and effectiveness of a symptomatic approach based on symptoms, administered by a nurse working in women's health area, in identifying vaginal infections in women with or without vaginal discharge. | 2009 | 19284438 |
| prevalence of vulvovaginitis and bacterial vaginosis in patients with koilocytosis. | empirical discussion regarding an association between koilocytosis and vulvovaginitis often occurs. thus, the objective of this study was to assess the prevalence of microorganisms associated with bacterial vaginosis and vulvovaginitis in women with and without koilocytosis. | 2008 | 19274321 |
| horizontal gene transfer in eukaryotic parasites: a case study of entamoeba histolytica and trichomonas vaginalis. | over the past few years it has become apparent that horizontal gene transfer (hgt) has played an important role in the evolution of pathogenic prokaryotes. what is less clear is the exact role that hgt has played in shaping the metabolism of eukaryotic organisms. the main problems are the reliable inference of hgt on a genomic scale as well as the functional assignment of genes in these poorly studied organisms. we have screened the completed genomes of the protists entamoeba histolytica and tri ... | 2009 | 19271203 |
| prevalence of and risk factors for sexually-transmitted infections in hidden female sex workers. | we wanted to determine the age-specific prevalence of selected sexually transmitted infections while assessing the risk factors among hidden female sex workers (hfsw). | 2009 | 19269760 |
| anal intercourse among young heterosexuals in three sexually transmitted disease clinics in the united states. | to examine factors associated with heterosexual anal intercourse (ai). | 2009 | 19265740 |
| multiplex pcr testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and trichomonas and viral agent infections in sexually active australian women. | knowing the prevalence of potential etiologic agents of nongonococcal and nonchlamydial cervicitis is important for improving the efficacy of empirical treatments for this commonly encountered condition. we describe four multiplex pcrs (mpcrs), designated vdl05, vdl06, vdl07, and vdl09, which facilitate the detection of a wide range of agents either known to be or putatively associated with cervicitis, including cytomegalovirus (cmv), enterovirus (ev), epstein-barr virus (ebv), varicella-zoster ... | 2009 | 19261782 |
| nmr structural analysis of dna recognition by a novel myb1 dna-binding domain in the protozoan parasite trichomonas vaginalis. | the transcription regulator, tvmyb1, is the first myb family protein identified in trichomonas vaginalis. using an electrophoretic mobility shift assay, we defined the amino-acid sequence from lys(35) to ser(141) (tvmyb1(35-141)) as the minimal dna-binding domain, encompassing two myb-like dna-binding motifs (designated as r2 and r3 motifs) and an extension of 10 residues at the c-terminus. nmr solution structures of tvmyb1(35-141) show that both the r2 and r3 motifs adopt helix-turn-helix confo ... | 2009 | 19246540 |
| association of public versus private health care utilization and prevalence of trichomonas vaginalis in maringá, paraná, brazil. | to determine the association of public versus private health care utilization and prevalence of trichomonas vaginalis in maringá, paraná, brazil. | 2009 | 19225796 |
| population-based assessment of mycoplasma genitalium in vietnam--low prevalence among married women of reproductive age in a rural area. | to analyse the prevalence of mycoplasma genitalium infection in a population-based study among married women from a demographic surveillance site in a rural geographical area of vietnam. | 2009 | 19220645 |
| validation of an immunologic diagnostic kit for infectious vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis. | fempure is a kit for the rapid diagnosis of vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis, based on aggregation of latex particles joined to specific antibodies. the validation of the method involved the parameters specificity, detection limit, robustness, clinical sensitivity, and clinical specificity. also, samples analyzed in parallel by the validated test and other recognized tests conducted by external laboratory were included. the method was specific for the 3 ... | 2009 | 19216938 |
| human papillomaviruses and genital co-infections in gynaecological outpatients. | high grade hpv infections and persistence are the strongest risk factors for cervical cancer. nevertheless other genital microorganisms may be involved in the progression of hpv associated lesions. | 2009 | 19216747 |
| comparison between trichmonas vaginalis symptomatic and asymptomatic isolates effects in intravaginally infected rats. | in this study, histopathological and immunohistochemical changes of the posterior vaginal fornix's and upper portion of the vagina were compared on rats infected with symptomatic and asymptomatic human isolates. eighteen symptomatic and asymptomatic female isolates were used (nine/ each). two groups of infected female rats were included in this study (3 rats /isolate). the results showed that there were no differences between symptomatic and asymptomatic isolates in histopathological changes; t. ... | 2008 | 19209767 |
| infectivity of trichomonas vaginalis pseudocysts inoculated intra-vaginally in mice. | in addition to the trophozoite, pseudocyst is another morphological form which is recently identified among genitourinary trichomonads. although, this pseudocyst is competent to divide, its role in trichomonas life cycle has not yet been confirmed. in this study the ability of intra-vaginally inoculated t. vaginalis pseudocysts to induce trichomoniasis in infected mice was evaluated in comparison to the trophozoites. pseudocysts formation was induced by using thermal-freezing cycle method. the i ... | 2008 | 19209760 |
| synthesis and in vitro trichomonicidal, giardicidal and amebicidal activity of n-acetamide(sulfonamide)-2-methyl-4-nitro-1h-imidazoles. | two new series of imidazole derivatives (acetamides: 1-8 and sulfonamides: 9-15) were synthesized using a short synthetic route. compound 1 as well as the intermediate 16g were characterized by x-ray crystallography. imidazole derivatives 1-15 were tested in vitro against three unicellular parasites (giardia intestinalis, trichomonas vaginalis and entamoeba histolytica) in comparison with benznidazole (bzn) and metronidazole. compound 1 [n-benzyl-2-(2-methyl-4-nitro-1h-imidazol-1-yl)acetamide] w ... | 2009 | 19208443 |
| trichomonal sinusitis in an adolescent patient with multiple trauma. | trichomonal disease typically involves the genital and occasionally respiratory tracts. although exposure of the upper respiratory tract to infected genital secretions is not uncommon with contemporary sexual practices, trichomonal sinus disease has been rarely described. the present report describes the case of a healthy 17-year-old male admitted to an intensive care unit following multiple trauma, who developed purulent sinusitis on the 4th day of hospitalization. numerous trichomonads were no ... | 2009 | 19204647 |
| [utility of molecular biology techniques in the diagnosis of sexually transmitted diseases and genital infections]. | historically, the diagnosis of sexually transmitted diseases (stds) has been difficult. the introduction of molecular biology techniques in microbiological diagnosis and their application to non-invasive samples has produced significant advances in the diagnosis of these diseases. overall, detection of neisseria gonorrhoeae by molecular biology techniques provides a presumptive diagnosis and requires confirmation by culture in areas with a low prevalence. for chlamydia trachomatis infections, th ... | 2008 | 19195446 |
| genetic diversity of trichomonas vaginalis clinical isolates determined by ecori restriction fragment length polymorphism of heat-shock protein 70 genes. | restriction fragment length polymorphism (rflp) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (hsp70) hybridization probe with ecori-digested genomic dna was used in molecular typing of 129 trichomonas vaginalis isolates. results indicate that trichomonas organisms exhibit considerable polymorphism in their hsp70 rflp patterns. analysis of seven american type culture collection reference strains and 122 clinical isolates, including 84 isolates from jackson, mississ ... | 2009 | 19190222 |
| cysteine peptidase expression in trichomonas vaginalis isolates displaying high- and low-virulence phenotypes. | in the present study, we identified and characterized the cysteine peptidase (cp) profiles of trichomonas vaginalis isolates exhibiting high- and low-virulence phenotypes using a combination of two-dimensional sds-page (2de), tandem mass spectrometry (ms/ms), and data mining. seven of the eight cps identified belong to clan ca, family c1, cathepsin l-like cp, and one belongs to clan cd, family c13, asparaginyl endopeptidase-like cp. quantitative and qualitative differences in cp expression were ... | 2009 | 19186947 |
| synthesis and antiprotozoal activity of novel 1-methylbenzimidazole derivatives. | in this paper are reported the synthesis and antiprotozoal activity in vitro of 24 1-methylbenzimidazole derivatives (13-36) substituted at position 2 with aminocarbonyl, n-methylaminocarbonyl, n,n-dimethylaminocarbonyl, ethoxycarbonyl, 1-hydroxyethyl and acetyl groups, some of them with chlorine atoms at the benzenoid ring. compounds 13-36 were more active than metronidazole, the choice drug against giardia intestinalis and most of them against trichomonas vaginalis. the most active group of co ... | 2009 | 19186059 |
| comparison of aptima trichomonas vaginalis transcription-mediated amplification to wet mount microscopy, culture, and polymerase chain reaction for diagnosis of trichomoniasis in men and women. | we evaluated the performance characteristics of aptima trichomonas vaginalis (atv) transcription-mediated amplification (tma) for diagnosis of t vaginalis (tv) infection from female vaginal swab, endocervical swab, and urine specimens and from male urethral swab and urine specimens. performance of atv tma was compared with wet mount microscopy, culture, and polymerase chain reaction (pcr). | 2009 | 19185101 |
| can bacterial vaginosis help to find sexually transmitted diseases, especially chlamydial cervicitis? | this study was undertaken to establish reliable factors in order to identify chlamydial cervicitis among suspicious patients. between january and december 2007, 406 patients who were suspected to have cervicitis due to clinical symptoms, were tested with polymerase chain reaction (pcr) for chlamydia trachomatis (ct), vaginal ph and nugent score (ns) in our university hospital and related clinics. during the same period, 67 patients who were diagnosed as having other sexually transmitted diseases ... | 2009 | 19182056 |
| [trichomoniasis]. | trichomonas vaginalis is the most common curable sexually transmitted disease worldwide. in spite of serious health sequelae including facilitation of hiv transmission, pid, and adverse outcomes of pregnancy, it remains an underestimated condition, as a half of female infections and the majority of male infections are asymptomatic. t. vaginalis infection was not restricted in high-risk groups, so screening or empiric treatment of low-risk groups should be considered. to develop diagnostic tests ... | 2009 | 19177767 |
| engineering escherichia coli for fermentative dihydrogen production: potential role of nadh-ferredoxin oxidoreductase from the hydrogenosome of anaerobic protozoa. | trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for h2 production. pyruvate ferredoxin oxidoreductase and nadh dehydrogenase (nadh-dh) are the two enzymes catalyzing the production of reduced ferredoxin. the genes encoding the two subunits of nadh-dh were cloned and expressed in escherichia coli. kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. th ... | 2009 | 19172236 |
| beyond douching: use of feminine hygiene products and sti risk among young women. | use of feminine hygiene products (feminine wipes, sprays, douches, and yeast creams) by adolescent women is common, yet understudied. | 2009 | 19170863 |
| cervicovaginal cytological abnormalities in patients with human immunodeficiency virus infection, in relation to disease stage, cd4 cell count and viral load. | the objective of the present study was to assess infections and cytologic abnormalities in cervicovaginal smears from 153 hiv-positive women and 169 hiv-negative followed up at the uftm school of medicine between may 1999 and may 2002. the medical records and cervicovaginal smears were reviewed and the hiv-positive group was classified according to cd4 cell count, hiv viral load, antiretroviral therapy and hiv subgroups (with or without disease; with or without therapy) and compared to hiv-negat ... | 2009 | 19170167 |
| [genetic variation and clustal analysis of trichomonas vaginalis cysteine proteases]. | to clone the genes coding for cysteine proteases (cps, tvcps) from trichomonas vaginalis and to analyze their genetic variations with the related sequences from ncbi database (genbank) and t. vaginalis genome project database from the institute for genomic research (tigr). | 2008 | 19160965 |
| [observation on mitosis of trichomonas vaginalis cultivated in vitro using modified giemsa staining]. | the observation showed that the percentage of trichomonas vaginalis trophozoites at the stages of interphase, binary fission and multiple fission was 66.5%, 24.1% and 9.4% respectively. cells in binary fission could be classified as premitotic phase, prophase, metaphase, anaphase and telophase. 3 to 8 microcosms were seen in one trophozoite under multiple fission and the percentage of trophozoites with 3 and 4 microcosms occupied 69% and 24.5% respectively. cells with abnormal morphs were also o ... | 2008 | 19157311 |
| [killing effect of polymorphonuclear neutrophils on trichomonas vaginalis]. | to study the killing effect of polymorphonuclear neutrophils (pmns) on trichomonas vaginalis. | 2008 | 19157301 |
| a comparison of cytological and parasitological methods in the diagnosis of trichomonas vaginalis. | trichomonas vaginalis (t. vaginalis), which causes urogenital system infections in humans, develops symptomatically and asymptomatically. t. vaginalis in females is diagnosed using direct microscopy, giemsa staining, and cultivation methods for examination of samples derived from the vaginal posterior fornix. serologic methods can also be employed. in cytological diagnosis, the ectocervical smear is examined using the papanicolaou (paps) stain. the aim of the present study was to compare the eff ... | 2008 | 19156601 |
| identification and molecular characterization of numerous histomonas meleagridis proteins using a cdna library. | summaryhistomonas meleagridis is a protozoan parasite of various galliform birds causing a type of enterohepatitis termed histomonosis or 'blackhead disease'. due to the ban of chemotherapeutic substances and an increase in free-range poultry production, histomonosis is currently a re-emerging disease. so far limited molecular knowledge is available. in the present work, mrnas coding for antigenic proteins of h. meleagridis were identified. for this purpose, a cdna expression library was constru ... | 2009 | 19154645 |
| transcriptional regulation of an iron-inducible gene by differential and alternate promoter entries of multiple myb proteins in the protozoan parasite trichomonas vaginalis. | iron-inducible transcription of a malic enzyme gene (also reputed to be ap65-1) in trichomonas vaginalis was previously shown to involve a myb1 repressor and a myb2 activator, each of which may preferentially select two closely spaced promoter sites, mre-1/mre-2r, which comprises overlapping promoter elements, and mre-2f. in the present study, an iron-inducible approximately 32-kda myb3 nuclear protein was demonstrated to bind only the mre-1 element. changes in the iron supply, which produced an ... | 2009 | 19151329 |
| current issues and considerations regarding trichomoniasis and human immunodeficiency virus in african-americans. | trichomonas vaginalis has long been recognized as one of the most prevalent sexually transmitted infections. however, it is only in recent years that it has been appreciated that trichomonas may play a critical role in amplifying human immunodeficiency virus (hiv) transmission. given the evidence that t. vaginalis likely promotes hiv infection, the apparent high level of trichomonas infection in the african-american community is cause for concern. even if t. vaginalis increases the risk of hiv t ... | 2009 | 19136432 |
| patient teenagers? a comparison of the sexual behavior of virginity pledgers and matched nonpledgers. | the us government spends more than $200 million annually on abstinence-promotion programs, including virginity pledges. this study compares the sexual activity of adolescent virginity pledgers with matched nonpledgers by using more robust methods than past research. | 2009 | 19117832 |
| trichomonosis and subsequent risk of prostate cancer in the prostate cancer prevention trial. | we previously observed a positive association between a history of trichomonosis, a sexually transmitted infection caused by the protozoan, trichomonas vaginalis, and prostate cancer risk in the health professionals follow-up study. to determine the reproducibility of this finding, we conducted a second, prospective investigation of trichomonosis and prostate cancer in the prostate cancer prevention trial. participants were men (>or=55 years of age) with no evidence of prostate cancer at enrollm ... | 2009 | 19117055 |
| persistent, undetected trichomonas vaginalis infections? | 2009 | 19113985 | |
| trichomonas vaginalis: the adhesins ap51 and ap65 bind heme and hemoglobin. | trichomonas vaginalis is the cause of human trichomoniasis, the most common non-viral sexually transmitted disease worldwide. although acquisition of iron by binding to host hemoglobin through distinct receptor(s) has been described, no specific heme- or hemoglobin-binding site has been reported in this parasite. to determine the presence of hemoglobin-binding protein(s), membrane proteins were subjected to hemoglobin-affinity chromatography. eluted proteins were analysed by sds-page. two protei ... | 2009 | 19105955 |
| accuracy of the clinical diagnosis of vaginitis compared with a dna probe laboratory standard. | to estimate the accuracy of the clinical diagnosis of the three most common causes of acute vulvovaginal symptoms (bacterial vaginosis, candidiasis vaginitis, and trichomoniasis vaginalis) using a traditional, standardized clinical diagnostic protocol compared with a dna probe laboratory standard. | 2009 | 19104364 |
| acetate:succinate coa-transferase in the anaerobic mitochondria of fasciola hepatica. | fasciola hepatica contains anaerobically functioning mitochondria that produce acetate and propionate, the main endproducts excreted by this parasite. the final reactions in the pathways leading to these endproducts are performed by acetate:succinate coa-transferase (asct) and propionate:succinate coa-transferase (psct), respectively. the enzymes catalysing these essential reactions in anaerobic mitochondria are still not characterized, nor are the corresponding genes identified. here we describ ... | 2009 | 19103231 |
| partial sequence of the beta-tubulin of histomonas meleagridis and the activity of benzimidazoles against h. meleagridis in vitro. | the protozoan parasite histomonas meleagridis is a member of the family monocercomonadidae in the class trichomonada. due to food safety concerns, currently no prophylactic or therapeutic drug against the parasite is licensed in the european union. benzimidazoles are antiparasitic drugs, and some of them are licensed for use in food-producing animals. benzimidazoles act on beta-tubulin, and the beta-tubulin sequence allows predictions about the efficacy of benzimidazoles. in this study, we seque ... | 2009 | 19101732 |
| reductive evolution of the mitochondrial processing peptidases of the unicellular parasites trichomonas vaginalis and giardia intestinalis. | mitochondrial processing peptidases are heterodimeric enzymes (alpha/betampp) that play an essential role in mitochondrial biogenesis by recognizing and cleaving the targeting presequences of nuclear-encoded mitochondrial proteins. the two subunits are paralogues that probably evolved by duplication of a gene for a monomeric metallopeptidase from the endosymbiotic ancestor of mitochondria. here, we characterize the mpp-like proteins from two important human parasites that contain highly reduced ... | 2008 | 19096520 |
| in vitro testing of rationally designed spermicides for selectively targeting human sperm in vagina to ensure safe contraception. | rational synthesis of novel structures resulted in two unique molecules (dse-36 and dse-37, disulphide esters of carbothioic acid) that killed sperm 25 times more strongly and with a precisely targeted action than nonoxynol-9 (n-9). we examine the effects of dse-36 and dse-37 on human spermatozoa versus hela cells to establish specificity and safety compared with n-9. | 2009 | 19088111 |
| male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis: observations after a randomised controlled trial for hiv prevention. | to assess the association between male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis using data from a male circumcision randomised controlled trial. | 2009 | 19074928 |