Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| rhodococcus equi can survive a phagolysosomal environment in macrophages by suppressing acidification of the phagolysosome. | rhodococcus equi is one of the most important causes of pneumonia in foals and has emerged as a significant opportunistic pathogen of immunosuppressed hosts such as human immunodeficiency virus-infected patients. virulent r. equi harbouring an 85 kb plasmid, but not the avirulent form lacking the plasmid, has the ability to survive in macrophages. however, the survival mechanism is not known. in the present study, morphological interactions were observed between virulent or plasmid-cured avirule ... | 2005 | 16192430 |
| septic pleuritis and abdominal abscess formation caused by rhodococcus equi in a foal. | a 3-month-old female arabian horse was evaluated because of fever, respiratory distress, lethargy, and decreased appetite of 5 days' duration. pleural effusion was diagnosed on the basis of ultrasonographic and radiographic examinations. cytologic examination of pleural fluid collected via thoracocentesis revealed septic inflammation; bacteriologic culture of a sample of that fluid yielded rhodococcus equi. a large intra-abdominal mass adjacent to the body wall was identified ultrasonographicall ... | 2005 | 16190597 |
| molecular and biochemical analysis of phthalate and terephthalate degradation by rhodococcus sp. strain dk17. | alkylbenzene-degrading rhodococcus sp. strain dk17 is able to utilize phthalate and terephthalate as growth substrates. the genes encoding the transformation of phthalate and terephthalate to protocatechuate are organized as two separate operons, located 6.7kb away from each other. interestingly, both the phthalate and terephthalate operons are induced in response to terephthalate while expression of the terephthalate genes is undetectable in phthalate-grown cells. in addition to two known plasm ... | 2005 | 16181748 |
| changes in structure of lymphatic organs in foals died due to rhodococcus equi infection. | the aim of this study was to investigate the structure of lymphatic organs in foals which died due to rhodococcus equi infection. the material for this study consisted of 9 foals, of both genders, various breeds, 6-14 weeks old. the routine histopathological examination and immunocytochemical reaction for cytokeratine-3 receptors revealed the disturbances in the thymus epithelial cells development. this was manifested by a complete lack of cells expressing cytokeratine-3 receptors, as well as ab ... | 2005 | 16180583 |
| protective role of neutrophils in mice experimentally infected with rhodococcus equi. | neutrophils are important in controlling early infections with the intracellular bacterium rhodococcus equi. antineutrophil monoclonal antibody (rb6-8c5)-induced neutrophil deficiency during the first week after experimental infection of mice with r. equi resulted in more severe disease and significantly increased tissue concentrations of r. equi. | 2005 | 16177388 |
| isocitrate lyase activity is required for virulence of the intracellular pathogen rhodococcus equi. | rhodococcus equi is an important pathogen of foals, causing severe pyogranulomatous pneumonia. virulent r. equi strains grow within macrophages, a process which remains poorly characterized. a potential source of carbon for intramacrophage r. equi is membrane lipid-derived fatty acids, which following beta oxidation are assimilated via the glyoxylate bypass. to assess the importance of isocitrate lyase, the first enzyme of the glyoxylate bypass, in virulence of a foal isolate of r. equi, a mutan ... | 2005 | 16177351 |
| evaluation of a multiplex polymerase chain reaction assay for simultaneous detection of rhodococcus equi and the vapa gene. | to evaluate sensitivity and specificity of a multiplex polymerase chain reaction (pcr) assay for simultaneous detection of rhodococcus equi and differentiation of strains that contain the virulence-associated gene (vapa) from strains that do not. | 2005 | 16173481 |
| atrazine degradation by encapsulated rhodococcus erythropolis ni86/21. | to develop an encapsulation procedure for rhodococcus erythropolis ni86/21 and demonstrate its use as a slow-release inoculant for reducing atrazine levels in aquatic and terrestrial environments. | 2005 | 16162227 |
| the signature amidase from sulfolobus solfataricus belongs to the cx3c subgroup of enzymes cleaving both amides and nitriles. ser195 and cys145 are predicted to be the active site nucleophiles. | the signature amidase from the extremophile archeum sulfolobus solfataricus is an enantioselective enzyme that cleaves s-amides. we report here that this enzyme also converts nitriles in the corresponding organic acid, similarly to the well characterized amidase from rhodococcus rhodochrous j1. the archaeal and rhodococcal enzymes belong to the signature amidases and contain the typical serine-glycine rich motif. they work at different optimal temperature, share a high sequence similarity and bo ... | 2005 | 16156792 |
| constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc. | the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ... | 2006 | 16156722 |
| comparison of selective media for the isolation of rhodococcus equi and description of a new selective plating medium. | in order to improve the isolation rate of rhodococcus equi from animals and soil, the efficacy of four previously described selective media (caz-nb, m3t, nanat and tinsdale) and that of four other media (nc, pnp, tcp and tvp) composed by us was compared and evaluated. two selective plating media proved to be the best for the isolation of r. equi from contaminated samples. one of them was caz-nb containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed tcp conta ... | 2005 | 16156123 |
| rhodococcus sp. f92 immobilized on polyurethane foam shows ability to degrade various petroleum products. | this work reports on the immobilization and performance of a hydrocarbon-degrading microorganism on polyurethane foam (puf) in the bioremediation of petroleum hydrocarbons. the ability of four different microorganisms to immobilize on puf and to degrade various petroleum products (arabian light crude (alc), al-shaheen crude (asc), diesel and oil slops) was assessed by measuring the n-alkane fraction remaining in the petroleum products over time. a rhodococcus sp. (designated as f92) had the high ... | 2006 | 16154500 |
| extracellular dna in single- and multiple-species unsaturated biofilms. | the extracellular polymeric substances (eps) of bacterial biofilms form a hydrated barrier between cells and their external environment. better characterization of eps could be useful in understanding biofilm physiology. the eps are chemically complex, changing with both bacterial strain and culture conditions. previously, we reported that pseudomonas aeruginosa unsaturated biofilm eps contains large amounts of extracellular dna (edna) (r. e. steinberger, a. r. allen, h. g. hansma, and p. a. hol ... | 2005 | 16151131 |
| isolation and characterization of a rhodococcus species strain able to grow on ortho- and para-xylene. | rhodococcus sp. strain yu6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. unlike most other o-xylene-degrading bacteria, yu6 is able to grow on p-xylene. numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that yu6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. this leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pa ... | 2005 | 16145546 |
| in vitro and intra-macrophage gene expression by rhodococcus equi strain 103. | rhodococcus equi is a facultative intracellular respiratory pathogen of foals that persists and multiplies within macrophages. in foals, virulence is associated with 80-90 kb plasmids, which include a pathogenicity island (pi) containing the virulence-associated protein (vap) gene family, but detailed understanding of the basis of virulence is still poor. a 60 spot-based dna microarray was developed containing eight pi genes and 42 chromosomal putative virulence or virulence-associated genes sel ... | 2005 | 16143469 |
| detection of equine herpesvirus type 1 using a real-time polymerase chain reaction. | equid herpesvirus 1 (ehv1) is a major disease of equids worldwide causing considerable losses to the horse industry. a variety of techniques, including pcr have been used to diagnose ehv1. some of these pcrs were used in combination with other techniques such as restriction enzyme analysis (rea) or hybridisation, making them cumbersome for routine diagnostic testing and increasing the chances of cross-contamination. furthermore, they involve the use of suspected carcinogens such as ethidium brom ... | 2006 | 16137772 |
| identification and structural characterisation of novel trehalose dinocardiomycolates from n-alkane-grown rhodococcus opacus 1cp. | rhodococcus opacus 1cp, a potent degrader of (chloro-) aromatic compounds was found to utilise c10-c16 n-alkanes as sole carbon sources. highest conversion rates were observed with n-tetradecane and n-hexadecane, whereas the utilisation of n-dodecane and n-decane was considerably slower. thin-layer chromatography of organic extracts of n-alkane-grown 1cp cultures indicated the growth-associated formation of a glycolipid which was characterised as a trehalose dimycolate by 1h-nmr spectroscopy and ... | 2006 | 16133336 |
| metabolic engineering for synthesis of aryl carotenoids in rhodococcus. | rhodococcus erythropolis naturally synthesizes monocyclic carotenoids: 4-keto-gamma-carotene and gamma-carotene. the genes and the pathway for carotenoid synthesis in r. erythropolis were previously described. we heterologously expressed a beta-carotene desaturase gene (crtu) from brevibacterium in rhodococcus to produce aryl carotenoids such as chlorobactene. expression of the crtu downstream of a chloramphenicol resistance gene on prhbr171 vector showed higher activity than expression downstre ... | 2006 | 16133327 |
| pyogranuloma caused by mycobacterium asiaticum infection in a steer. | in 2003, a steer carcass was condemned at a central queensland abattoir because of metastatic tumors. in addition, a granulomatous lesion was found in the mediastinal lymph node. histological examination showed this to be a pyogranuloma, typically associated with rhodococcus or the nocardia/streptomyces group. however, in this case, the only etiological agent was an acid-fast bacillus, which would normally be associated with a more fibrous lesion. a number of nucleic acid-based techniques were u ... | 2005 | 16130999 |
| in vitro activity and killing effect of uperin 3.6 against gram-positive cocci isolated from immunocompromised patients. | the in vitro activity of uperin 3.6, alone or combined with six antibiotics, against gram-positive cocci, including rhodococcus equi, methicillin-resistant staphylococci, and vancomycin-resistant enterococci, was investigated. all isolates were inhibited at concentrations of 1 to 16 mg/liter. synergy was demonstrated when uperin 3.6 was combined with clarithromycin and doxycycline. | 2005 | 16127075 |
| [the microbial transformation of phenanthrene and anthracene]. | the transformation of phenanthrene and anthracene by rhodococcus rhodnii 135, pseudomonas fluorescens 26k, and arthrobacter sp. k3 is studied. twenty-one intermediates of phenanthrene and anthracene transformation are identified by hplc, mass spectrometry, and nmr spectroscopy. p. fluorescens 26k and arthrobacter sp. k3 are found to produce a wide range of intermediates, whereas r. rhodnii 135 oxidizes phenanthrene, resulting in the formation of a sole product, 3-hydroxyphenanthrene. putative tr ... | 2005 | 16119849 |
| isotopic fractionation indicates anaerobic monochlorobenzene biodegradation. | the concentration and isotopic composition of monochlorobenzene (mcb) was monitored in the plume of an anaerobic, contaminated aquifer in bitterfeld, germany. an enrichment in the carbon isotopic composition of more than 4 delta units was found at the fringes of the plume relative to the center (-26.5 %), suggesting the occurrence of in situ biodegradation of mcb. a similar enrichment was measured in a detailed cross-section of the plume and in depth-specific samples obtained in a multilevel sam ... | 2005 | 16117106 |
| virulence-associated protein-specific serum immunoglobulin g-isotype expression in young foals protected against rhodococcus equi pneumonia by oral immunization with virulent r. equi. | the purpose of this study was to determine whether foals immunized orally from 2 days of age with virulent rhodococcus equi developed a protective pulmonary immune response and to characterise the antibody response of the immunized foals to the virulence-associated proteins (vaps) of the bacterium. two groups of foals were used. one (n=4) was given live r. equi atcc 33701 orally at 2, 7, and 14 days of age. the second group comprised three non-immunized foals age-matched to the vaccinates. at 3 ... | 2005 | 16112256 |
| rhodococcus equi pneumonia in aids: high-resolution ct findings in five patients. | the aim of this study was to describe the high-resolution ct scan findings in five patients with aids and pulmonary infection due to rhodococcus equi. the study included five patients with aids and proven r. equi infection. the ct scans were reviewed by two observers. the patients included four men and one woman ranging from 39 years to 49 years in age (mean 42 years). the findings included areas of consolidation (n=5) with single (n=1) or multiple cavitation (n=4), ground-glass opacities (n=5), ... | 2005 | 16110097 |
| construction of random transposition mutagenesis system in rhodococcuserythropolis using is1415. | recent studies on the metabolic activities of genus rhodococcus have shown rhodococci to be of important use in industrial, pharmaceutical and environmental biotechnology. the increasing economic significance of rhodococcus encourages renewed efforts to characterize their genetic systems, as rhodococcus genetics are still poorly understood. the goal of this study is to adapt a transposon system for use in creating random mutagenesis in rhodococcus erythropolis. a plasmid carrying is1415, a membe ... | 2006 | 16107286 |
| rapid detection and identification of the metabolically diverse genus gordonia by 16s rrna-gene-targeted genus-specific primers. | the importance of the emerging genus gordonia in industrial and environmental biotechnology is evidenced by the recent increase in associated publications and patents. but, investigations into potentially valuable gordonia members are restricted by the limitations of current isolation and detection techniques. this motivated us to design a genus-specific oligonucleotide primer pair which could assist in rapid detection of species of the genus gordonia by means of pcr-specific amplification. the ... | 2005 | 16098691 |
| metabolism of the aliphatic nitramine 4-nitro-2,4-diazabutanal by methylobacterium sp. strain js178. | the aliphatic nitramine 4-nitro-2,4-diazabutanal (ndab; c2h5n3o3) is a ring cleavage metabolite that accumulates during the aerobic degradation of the energetic compound hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by various rhodococcus spp. ndab is also produced during the alkaline hydrolysis of either rdx or octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (hmx) and during the photolysis of rdx. traces of ndab were observed in a soil sampled from an ammunition-manufacturing facility contamin ... | 2005 | 16085803 |
| characterization of microorganisms isolated from lignite excavated from the záhorie coal mine (southwestern slovakia). | microorganisms were isolated from lignite freshly excavated in the záhorie coal mine (southwestern slovakia) under conditions excluding contamination with either soil or air-borne microorganisms. the isolates represented both prokarya and eukarya (fungi). all were able to grow on standard media, although some microorganisms were unstable and became extinct during storage of coal samples. bacteria belonged to the genera bacillus, staphylococcus, and rhodococcus, according to both morphological cr ... | 2005 | 16085397 |
| novel microbial epoxide hydrolases for biohydrolysis of glycidyl derivatives. | microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous fungi, four were selected based on the enantioselectivities of their epoxide hydrolases determined in biotransformation reactions. the enzyme of aspergillus niger m200 preferentiall ... | 2005 | 16061300 |
| degradation of aflatoxin b(1) by cell-free extracts of rhodococcus erythropolis and mycobacterium fluoranthenivorans sp. nov. dsm44556(t). | biological degradation of aflatoxin b(1) (afb(1)) by rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. dramatic reduction of afb(1) was observed during incubation in the presence of r. erythropolis cells (17% residual afb(1) after 48 h and only 3-6% residual afb(1) after 72 h). cell-free extracts of four bacterial strains, r. erythropolis dsm 14,303, nocardia corynebacterioides dsm 12,676, n. corynebacterioides dsm 20,151, and mycobacterium fluoranthenivorans sp ... | 2005 | 16061299 |
| genetic diversity of culturable bacteria in oil-contaminated rhizosphere of galega orientalis. | a collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16s rdna partial sequencing showed the presence of five major lineages of the bacteria domain. gram-positive rhodococcus, bacillus and arthrobacter and gram-negative pseudomonas were the most abundant genera. only one-fifth of the strains that tolerated m-toluate also degraded m-tolu ... | 2006 | 16055251 |
| structure of an atypical epoxide hydrolase from mycobacterium tuberculosis gives insights into its function. | epoxide hydrolases are vital to many organisms by virtue of their roles in detoxification, metabolism and processing of signaling molecules. the mycobacterium tuberculosis genome encodes an unusually large number of epoxide hydrolases, suggesting that they might be of particular importance to these bacteria. we report here the first structure of an epoxide hydrolase from m.tuberculosis, solved to a resolution of 2.5 a using single-wavelength anomalous dispersion (sad) from a selenomethionine-sub ... | 2005 | 16051262 |
| isolation of a bacterium that degrades urethane compounds and characterization of its urethane hydrolase. | a bacterium which degrades urethane compounds was isolated and identified as rhodococcus equi strain tb-60. strain tb-60 degraded toluene-2,4-dicarbamic acid dibutyl ester (tdcb) and accumulated toluene diamine as the degradation product. the enzyme which cleaves urethane bond in tdcb was strongly induced by acetanilide. the purified enzyme (urethane hydrolase) was found to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weight was estimated to be 55 kd ... | 2006 | 16041575 |
| the association between weather, frenzied behaviour, percutaneous invasion by strongyloides westeri larvae and rhodococcus equi disease in foals. | episodes of frenzy lasting approximately 30 minutes were observed among horses confined to enclosures surfaced with sand or soil. the probability of sighting these episodes increased by a factor of three when within 24 hours there was 0.2mm or more of rain, a maximum air temperature between 16.7 - 26.6 degrees c and a soil temperature of 16.3 - 23.9 degrees c at 30 cm. high egg counts of strongyloides westeri appeared in faeces four to five days later and persisted for several days. rhodococcus ... | 1989 | 16031523 |
| distribution of rhodococcus equi in animals, birds and from the environment. | 1987 | 16031395 | |
| endosulfan degradation by a rhodococcus strain isolated from earthworm gut. | a rhodococcus mtcc 6716 bacterial strain was isolated apparently for the first time from the gut microflora of an indian earthworm (metaphire posthuma). endosulfan was used as a carbon source by the strain and degraded it up to 92.58% within 15 days. furthermore, the isolated strain of the bacterium did not produce the persistent form of the toxic metabolite endosulfan sulfate. this strain exhibits luxury growth in minimal medium with high concentrations of endosulfan (80 microg ml(-1)). degrada ... | 2006 | 16029891 |
| novel mycolic acid-containing bacteria in the family segniliparaceae fam. nov., including the genus segniliparus gen. nov., with descriptions of segniliparus rotundus sp. nov. and segniliparus rugosus sp. nov. | four strains of novel, rapidly growing, acid-alcohol-fast-staining bacteria were characterized with a polyphasic approach. isolates were received by the centers for disease control and prevention from domestic health department laboratories for reference testing as unidentifiable, clinical mycobacteria. bacteria were rod-shaped and produced non-pigmented (white to beige), non-photochromogenic, smooth or wrinkled-rough colonies on middlebrook 7h10 and 7h11 media at 33 degrees c. the smooth and wr ... | 2005 | 16014491 |
| rhodococcus triatomae sp. nov., isolated from a blood-sucking bug. | two bacterial isolates, strains immib riv-085(t) and immib riv-095, isolated from a blood-sucking bug of the genus triatoma, were characterized by phenotypic and molecular taxonomic methods. chemotaxonomic investigations revealed the presence of cell-wall chemotype iv and mycolic acids consistent with the genus rhodococcus. comparative 16s rrna gene sequencing showed that the two isolates are genealogically highly related (100% sequence similarity) and constitute a new subline within the genus r ... | 2005 | 16014484 |
| maturation of rhodococcus equi-containing vacuoles is arrested after completion of the early endosome stage. | rhodococcus equi is a facultative intracellular bacterium that can cause bronchopneumonia in foals and aids patients. here, we have analyzed r. equi-containing vacuoles (rcvs) in murine macrophages by confocal laser scanning microscopy, by transmission electron microscopy and by immunochemistry upon purification. we show that rcvs progress normally through the early stages of phagosome maturation acquiring pi3p, early endosome antigen-1, and rab5, and loosing all or much of them within minutes. ... | 2005 | 15998320 |
| the absence of rhodococcus equi in mongolian horses. | in native mongolian horses, the incidence and distribution of rhodococcus equi are poorly understood. one hundred and fourteen equine fecal samples and 71 soil samples were collected from the camp sites of 26 nomadic families located in three areas less than 100 km from ulaanbaatar, mongolia. five fecal samples were also collected from foals of przewalski's horses introduced into the hustai national park, mongolia. no r. equi was isolated from the mongolian horses or the soil samples. however, t ... | 2005 | 15997190 |
| gas vesicles in actinomycetes: old buoys in novel habitats? | gas vesicles are gas-filled prokaryotic organelles that function as flotation devices. this enables planktonic cyanobacteria and halophilic archaea to position themselves within the water column to make optimal use of light and nutrients. few terrestrial microbes are known to contain gas vesicles. genome sequences that have become available recently for many bacteria from non-planktonic habitats reveal gas vesicle gene clusters in members of the actinomycete genera streptomyces, frankia and rhod ... | 2005 | 15993071 |
| in vitro activity of citropin 1.1 alone and in combination with clinically used antimicrobial agents against rhodococcus equi. | the aim of this study was to investigate the in vitro activity of citropin 1.1, an antimicrobial peptide derived from the australian tree frog litoria citropa, alone and in combination with ampicillin, ceftriaxone, doxycycline, netilmicin, ciprofloxacin, rifampicin, linezolid, vancomycin, clarithromycin and imipenem against 12 nosocomial isolates of rhodococcus equi. | 2005 | 15983026 |
| phenylacetate catabolism in rhodococcus sp. strain rha1: a central pathway for degradation of aromatic compounds. | in gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (paa) that proceeds via phenylacetyl-coenzyme a (coa) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. in contrast, the paa pathway and its role are not well characterized in gram-positive bacteria. a cluster including 13 paa genes encoding enzymes orthologous to those of gram-negative bacteria was identified on the chromosome of rhodococcus sp. strain rha1. thes ... | 2005 | 15968060 |
| biodegradation and rhodococcus--masters of catabolic versatility. | the genus rhodococcus is a very diverse group of bacteria that possesses the ability to degrade a large number of organic compounds, including some of the most difficult compounds with regard to recalcitrance and toxicity. they achieve this through their capacity to acquire a remarkable range of diverse catabolic genes and their robust cellular physiology. rhodococcus appear to have adopted a strategy of hyper-recombination associated with a large genome. notably, they harbour large linear plasm ... | 2005 | 15961029 |
| management and visualization of whole genome shotgun assemblies using sam. | we have designed and implemented a system to manage whole genome shotgun sequences and whole genome sequence assembly data flow. the sequence assembly manager (sam) consists primarily of a mysql relational database and perl applications designed to easily manipulate and coordinate the analysis of sequence information and to view and report genome assembly progress through its common gateway interface (cgi) web interface. the application includes a tool to compare sequence assemblies to fingerpri ... | 2005 | 15945370 |
| adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases. | alkene monooxygenase (amo) from rhodococcus rhodochrous (formerly nocardia corallina) b-276 belongs to a family of multicomponent nonheme binuclear iron-centre oxygenases that includes the soluble methane monooxygenases (smmos) found in some methane-oxidizing bacteria. the enzymes catalyse the insertion of oxygen into organic substrates (mostly hydrocarbons) at the expense of o2 and nad(p)h. amo is remarkable in its ability to oxidize low molecular-mass alkenes to their corresponding epoxides wi ... | 2005 | 15943801 |
| evaluation of a real-time quantitative polymerase chain reaction assay for detection and quantitation of virulent rhodococcus equi. | to evaluate a real-time quantitative polymerase chain reaction (qpcr) assay in the detection and quantitation of virulent rhodococcus equi. | 2005 | 15940818 |
| catabolism of benzoate and phthalate in rhodococcus sp. strain rha1: redundancies and convergence. | genomic and proteomic approaches were used to investigate phthalate and benzoate catabolism in rhodococcus sp. strain rha1, a polychlorinated biphenyl-degrading actinomycete. sequence analyses identified genes involved in the catabolism of benzoate (ben) and phthalate (pad), the uptake of phthalate (pat), and two branches of the beta-ketoadipate pathway (catrabc and pcajihgblfr). the regulatory and structural ben genes are separated by genes encoding a cytochrome p450. the pad and pat genes are ... | 2005 | 15937168 |
| nonconventional hydrolytic dehalogenation of 1-chlorobutane by dehydrated bacteria in a continuous solid-gas biofilter. | rhodococcus erythropolis ncimb 13064 and xanthobacter autotrophicus gj10 are able to catalyze the conversion of halogenated hydrocarbons to their corresponding alcohols. these strains are attractive biocatalysts for gas phase remediation of polluted gaseous effluents because of their complementary specificity for short or medium and for mono-, di-, or trisubstituted halogenated hydrocarbons (c2-c8 for rhodococcus erythropolis and c1-c4 for xanthobacter autotrophicus). after dehydration, these ba ... | 2005 | 15929125 |
| catabolism of mesamoll, a technical formulation of alkylsulfonic acid phenyl esters, by two strains of rhodococcus rhodochrous. | the bacterial catabolism of a technical formulation of alkylsulfonic acid phenyl esters employed as a general purpose plasticizer, mesamoll, was elucidated. two strains of the genus rhodococcus were found able to utilize this substrate mixture as sole source of carbon and energy. growth experiments along with enzymatic measurements indicated that both strains utilized the phenol that was released from the corresponding alkylsulfonic acid phenyl ester-probably by enzymatic hydrolysis catalyzed by ... | 2005 | 15921896 |
| the two-phase water/silicon oil bioreactor prospects in off-gas treatment. | research was carried out to develop a biphasic biologic reactor able to clean the gas effluents polluted by volatile organic compounds. initially, rhodococcus erythropolis t 902.1 was selected on the basis of its capacity to degrade isopropylbenzene (ipb). the effect of gas flow and ipb concentration on the biodegradation of ipb was evaluated. the results show that the use of silicon oil allows large quantities of ipb to be absorbed within the medium of biologic abatement. on the other hand, the ... | 2005 | 15920274 |
| molecular analyses of mycobacteria other than the m. tuberculosis complex isolated from northern ireland cattle. | mycobacteria other than the mycobacterium tuberculosis complex (mott), isolated from northern ireland cattle, were identified by pcr amplification of the 16s rrna gene, and subsequent reverse cross blot hybridisation and sequence analyses. elucidation of the mott species was to facilitate specificity testing of new and existing diagnostic test reagents for bovine tuberculosis. the presence of the genes for potential diagnostic antigens: mpb70, mpb64, esat-6 and cfp-10 in the isolated mott specie ... | 2005 | 15917138 |
| polymerase chain reaction for identification of aldoxime dehydratase in aldoxime- or nitrile-degrading microorganisms. | we developed a molecular screening procedure using southern hybridization and polymerase chain reaction (pcr) to identify aldoxime dehydratase (oxd) encoding genes (oxds) among 14 aldoxime- or nitrile-degrading microorganisms. when an oxd gene of rhodococcus erythropolis n-771 was used as a probe, positive hybridization signals were seen with the chromosomal dna of eight strains, suggesting that these strains have similar oxd genes to r. erythoropolis n-771. by analyzing the pcr-amplified fragme ... | 2005 | 15899412 |
| study of serum amyloid a concentrations as a means of achieving early diagnosis of rhodococcus equi pneumonia. | prognosis of rhodococcus equi pneumonia can be challenging because the course of the disease is often insidious and overt clinical signs are subtle. early diagnosis is considered desirable because it may offer the chance of more successful implementation of treatment and, thereby, improved outcome. serological tests have previously failed to be accurate for early detection or diagnosis. measurement of serum amyloid a (saa) prior to and at the time of clinical signs was therefore chosen in order ... | 2005 | 15892228 |
| [necrotizing pneumonia due to rhodococcus equi in non hiv immunocompromised host. case report and review]. | rhodococcus equi, is a grampositive intracellular bacillus, that causes infection mainly in immunocompromised hosts. we report the case of a 52 years old woman, with a systemic lupus erythematosus and a progressive 10 months evolution with cough, dyspnea, mucous-purulent sputum, occasionally hemoptysis, intermittent fever, and weight loss of 10%. with partial response to antibiotic treatment, radiological evolution of thoracic scanners evidenced the development of multiple bilateral areas of con ... | 2005 | 15891796 |
| characterization of clinical isolates of pathogenic nocardia strains and related actinomycetes in thailand from 1996 to 2003. | in thailand from 1996 to 2003, 171 strains of pathogenic aerobic actinomycetes from clinical specimens were isolated. of those strains, 134 were mycolic acid containing actinomycetes, including 96 strains of nocardia species. others included 10 strains of gordonia, 14 strains of rhodococcus, and 22 strains of mycobacterium. one strain each of the genera tsukamurella and corynebacterium were also isolated. also identified were 27 strains of non-mycolic acid containing actinomycetes. our identific ... | 2005 | 15883719 |
| microbial metabolism of 2-chlorophenol, phenol and rho-cresol by rhodococcus erythropolis m1 in co-culture with pseudomonas fluorescens p1. | chlorophenolic waste most often contains phenol and rho-cresol along with chlorophenols. a rhodococcus erythropolis strain m1 was isolated with the ability to degrade 2-chlorophenol, phenol and p-cresol (100 mgl(-1), each) in 18, 24 and 20 h, respectively, with negligible lag. however, rhodococcus sp. characterized by low growth rate, pose a threat to be outgrown by bacteria occurring in natural habitats. in the present study, interaction of r. erythropolis m1 with another isolated bacteria gene ... | 2005 | 15881826 |
| the effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal. | the nitrilase of rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g ... | 2005 | 15880828 |
| reclassification of nocardia corynebacterioides serrano et al. 1972 (approved lists 1980) as rhodococcus corynebacterioides comb. nov. | the type strain of nocardia corynebacterioides was the subject of a polyphasic taxonomic study. the 16s rrna gene sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordonia, mycobacterium, nocardia, rhodococcus, skermania, tsukamurella and williamsia, and phylogenetic trees were constructed by using maximum-parsimony, maximum-likelihood and neighbour-joining methods. it was evident from the phylogenetic analysis that n. corynebacterioides represen ... | 2005 | 15879280 |
| rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. | a gram-positive, aerobic, non-motile, mesophilic strain, designated yim 70056(t), was isolated from a forest soil sample in yunnan province, china. phylogenetic analysis based on 16s rrna gene sequences revealed that this isolate had less than 97.0 % similarity to any rhodococcus species with validly published names, with the exception of rhodococcus fascians (dsm 20669(t)), which was found to be its closest neighbour (98.9 % similarity). chemotaxonomic data, including peptidoglycan type, diagno ... | 2005 | 15879245 |
| isolation and characterization of a diverse group of phenylacetic acid degrading microorganisms from pristine soil. | a diverse range of microorganisms capable of growth on phenylacetic acid as the sole source of carbon and energy were isolated from soil. sixty six different isolates were identified and grouped according to 16s rrna gene rflp analysis. subsequent sequencing of 16s rdna from selected strains allowed further characterization of the phenylacetic acid degrading population isolated from soil. nearly half (30) of the isolates are bacillus species while the rest of the isolates are strains from a vari ... | 2005 | 15869782 |
| the iron-regulated iupabc operon is required for saprophytic growth of the intracellular pathogen rhodococcus equi at low iron concentrations. | rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by r. equi to thrive under these conditions. this paper reports the characterization of an r. equi transposome mutant that fails to grow at low iron concentrations. the transposome was shown to be inserted into iupa ... | 2005 | 15866930 |
| [selectively desulfurizing organic sulfur of diesel oil by resting cells]. | rhodococcus sp. fs-1 ,which can specially break the c[single bond]s bond of dibenzothiophene (dbt) and convert dbt into 2-hydrobenzophene by "4s" pathway, is used to decrease the sulfur content in diesel oil. this research focuses on the diesel oil desulfurization using resting cells of rhodococcus sp. fs-1. the research result indicate that the desulfurization activity of the resting cells of rhodococcus sp. fs-1 was strongly high. when the concentration of dbt was 0.5 - 1.0mmol/l and the ratio ... | 2005 | 15861535 |
| adaptation of rhodococcus erythropolis dcl14 to growth on n-alkanes, alcohols and terpenes. | rhodococcus erythropolis dcl14 has the ability to convert the terpene (-)-carveol to the valuable flavour compound (-)-carvone when growing on a wide range of carbon sources. to study the effect of carbon and energy sources such as alkanes, alkanols and terpenes on the biotechnological process, the cellular adaptation at the level of fatty acid composition of the membrane phospholipids and the (-)-carvone production were examined. all tested carbon sources caused a dose-dependent increase in the ... | 2004 | 15856218 |
| transient accumulation of gamma-butyrolactone during degradation of bis(4-chloro-n-butyl) ether by diethylether-grown rhodococcus sp. strain dtb. | rhodococcus sp. strain dtb (dsm 44534) grows aerobically on diethylether as sole source of carbon and energy. dense cell suspension experiments showed that the induced ether-cleaving enzyme system attacks a broad range of ethers like tetrahydrofuran, phenetole and chlorinated alkylethers including calpha-substituted alkylethers. identification of metabolites revealed that degradation of the ethers started by an initial attack of the ether bond. diethylether-grown cells degraded bis(4-chloro-n-bu ... | 2005 | 15843929 |
| cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system. | carvone has previously been found to highly inhibit its own production at concentrations above 50 mm during conversion of a diastereomeric mixture of (-)-carveol by whole cells of rhodococcus erythropolis. adaptation of the cells to the presence of increasing concentrations of carveol and carvone in n-dodecane prior to biotransformation proved successful in overcoming carvone inhibition. by adapting r. erythropolis cells for 197 h, an 8.3-fold increase in carvone production rate compared to non- ... | 2005 | 15841371 |
| [dependence of the relative value of redox potential in rhodococcus rhodochrous cells on their growth rate in culture]. | 2005 | 15835791 | |
| [auxin production by bacteria associated with orchid roots]. | bacteria associated with the roots of greenhouse tropical orchids were shown to produce indole-3-acetic acid (iaa) and to excrete it into the culture liquid. the presence and activity of iaa were demonstrated colorimetrically, by thin-layer chromatography, and by biotests. the associated bacteria varied in their ability to excrete indole compounds (1-28 microg/ml nutrient broth). addition of tryptophan to the growth medium enhanced phytohormone production. upon addition of 200 microg/ml tryptoph ... | 2005 | 15835779 |
| modified electrodes based on lipidic cubic phases. | the lipidic cubic phase can be characterized as a curved bilayer forming a three-dimensional, crystallographical, well-ordered structure that is interwoven by aqueous channels. it provides a stable, well-organized environment in which diffusion of both water-soluble and lipid-soluble compounds can take place. cubic phases based on monoacylglycerols form readily and attract our interest due to their ability to incorporate and stabilize proteins. their lyotropic and thermotropic phase behaviour ha ... | 2005 | 15833697 |
| analysis of bacterial diversity in acidic pond water and compost after treatment of artificial acid mine drainage for metal removal. | the microbial population of a sludge amended leaf compost material utilized for treatment of artificial acid mine drainage was studied by culture-independent molecular methods. iron-rich and sulfurous wastewater (artificial acid mine drainage) was circulated through a column bioreactor for 16 months. after 12 months the column was inoculated with a mixed culture from an acidic pond receiving acid mine drainage from a tailings impoundment at a decommissioned site in kristineberg, north sweden. hy ... | 2005 | 15818559 |
| cloning, expression, and site-directed mutagenesis of the propene monooxygenase genes from mycobacterium sp. strain m156. | propene monooxygenase has been cloned from mycobacterium sp. strain m156, based on hybridization with the amoabcd genes of rhodococcus corallinus b276. sequencing indicated that the mycobacterial enzyme is a member of the binuclear nonheme iron monooxygenase family and, in gene order and sequence, is most similar to that from r. corallinus b-276. attempts were made to express the pmoabcd operon in escherichia coli and mycobacterium smegmatis mc(2)155. in the former, there appeared to be a proble ... | 2005 | 15812019 |
| web-type evolution of rhodococcus gene clusters associated with utilization of naphthalene. | clusters of genes which include determinants for the catalytic subunits of naphthalene dioxygenase (naraa and narab) were analyzed in naphthalene-degrading rhodococcus strains. we demonstrated (i) that in the region analyzed homologous gene clusters are separated from each other by nonhomologous dna, (ii) that there are various degrees of homology between related genes, and (iii) that nar genes are located on plasmids in strains ncimb12038 and p400 and on a chromosome in p200. these observations ... | 2005 | 15811998 |
| analysis of the domain properties of the novel cytochrome p450 rhf. | the properties of the heme, flavin mononucleotide (fmn) and fes domains of p450 rhf, from rhodococcus sp. ncimb 9784, expressed separately and in combination are analysed. the nucleotide preference, imidazole binding and reduction potentials of the heme and fmn domains are unaltered by their separation. the intact enzyme is monomeric and the flavin is confirmed to be fmn. the two one-electron reduction potentials of the fmn are -240 and -270 mv. the spectroscopic and thermodynamic properties of ... | 2005 | 15811344 |
| [use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil]. | oil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as acinetobacter calcoaceticus k-4, nocardia vaceinii k-8, rhodococcus erythropolis ek-1, and mycobacterium sp. k-2. it was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. it was also found that water polluted with 100 mg/l oil could be purified with rhodococcus erythropolis ek-1 and nocardia vaceinii k-8 cells im ... | 2005 | 15810734 |
| characterization of ltsa from rhodococcus erythropolis, an enzyme with glutamine amidotransferase activity. | the nocardioform actinomycete rhodococcus erythropolis has a characteristic cell wall structure. the cell wall is composed of arabinogalactan and mycolic acid and is highly resistant to the cell wall-lytic activity of lysozyme (muramidase). in order to improve the isolation of recombinant proteins from r. erythropolis host cells (n. nakashima and t. tamura, biotechnol. bioeng. 86:136-148, 2004), we isolated two mutants, l-65 and l-88, which are susceptible to lysozyme treatment. the lysozyme sen ... | 2005 | 15805504 |
| characterisation of the nitrile hydratase gene clusters of rhodococcus erythropolis strains aj270 and aj300 and microbacterium sp. aj115 indicates horizontal gene transfer and reveals an insertion of is1166. | the nitrile metabolising strains aj270, aj300 and aj115 were isolated from the same location. the strains have very similar nitrile metabolising profiles. sequencing of the 16s rrna gene indicates that strains aj270 and aj300 are novel strains of rhodococcus erythropolis while strain aj115 is a novel microbacterium strain very closely related to microbacterium oxydans and microbacterium liquefaciens. analysis of the structure of the nitrile hydratase/amidase gene clusters in the three strains in ... | 2005 | 15803388 |
| rhodococcus equi brain abscess in a patient without hiv. | rhodococcus equi, a gram positive organism, is a cause of infections in immunocompromised individuals. in humans, it mainly causes disease in those infected with human immunodeficiency virus (hiv), and generally presents as chronic pulmonary infection. it may also cause intracranial infections, which manifest as brain abscesses. this report describes a case of rhodococcus brain and pulmonary infection in a patient who did not have hiv or another disorder of cell mediated immunity. he was treated ... | 2005 | 15790711 |
| nitrile biotransformations for highly enantioselective synthesis of oxiranecarboxamides with tertiary and quaternary stereocenters; efficient chemoenzymatic approaches to enantiopure alpha-methylated serine and isoserine derivatives. | [reaction: see text] biotransformations of a number of differently substituted and configured oxiranecarbonitriles using rhodococcus sp. aj270, a microbial whole-cell catalyst that contains nitrile hydratase/amidase, were studied. while almost all trans-configured 3-aryl-2-methyloxiranecarbonitriles and 2,3-dimethyl-3-phenyloxiranecarbonitrile were efficiently hydrated by the action of the less enantioselective nitrile hydratase, the amidase exhibited excellent 2s,3r-enantioselectivity against 2 ... | 2005 | 15787529 |
| rhodococcus equi-specific cytotoxic t lymphocytes in immune horses and development in asymptomatic foals. | rhodococcus equi is an important cause of pneumonia in young horses; however, adult horses are immune due to their ability to mount protective recall responses. in this study, the hypothesis that r. equi-specific cytotoxic t lymphocytes (ctl) are present in the lung of immune horses was tested. bronchoalveolar lavage (bal)-derived pulmonary t lymphocytes stimulated with r. equi lysed infected alveolar macrophages and peripheral blood adherent cells (pbac). as with ctl obtained from the blood, ki ... | 2005 | 15784549 |
| aging of triazine amine in soils demonstrated through sorption, desorption, and bioavailability measurements. | the aging of triazine amine in soil was studied during a time course of 119 d by measuring bioavailability in terms of mineralization after inoculation of the triazine amine-degrading bacterium rhodococcus erythropolis ta57. the bioavailability was measured in four soil samples: a-, b-, and c-horizons from an agricultural soil profile and in a peat soil. the sorption of triazine amine in the soil samples was quantified during the period of aging in terms of sorption distribution coefficients (kd ... | 2005 | 15779748 |
| crystal structure of the hydroxyquinol 1,2-dioxygenase from nocardioides simplex 3e, a key enzyme involved in polychlorinated aromatics biodegradation. | hydroxyquinol 1,2-dioxygenase (1,2-hqd) catalyzes the ring cleavage of hydroxyquinol (1,2,4-trihydroxybenzene), a central intermediate in the degradation of aromatic compounds including a variety of particularly recalcitrant polychloro- and nitroaromatic pollutants. we report here the primary sequence determination and the analysis of the crystal structure of the 1,2-hqd from nocardioides simplex 3e solved at 1.75 a resolution using the multiple wavelength anomalous dispersion of the two catalyt ... | 2005 | 15772073 |
| gene expression systems in corynebacteria. | corynebacterium belongs to a group of gram-positive bacteria having moderate to high g+c content, the other members being mycobacterium, nocardia, and rhodococcus. considerable information is now available on the plasmids, gene regulatory elements, and gene expression in corynebacteria, especially in soil corynebacteria such as corynebacterium glutamicum. these bacteria are non-pathogenic and, unlike bacillus and streptomyces, are low in proteolytic activity and thus have the potential of becomi ... | 2005 | 15766862 |
| identification of two-component regulatory genes involved in o-xylene degradation by rhodococcus sp. strain dk17. | putative genes for a two-component signal transduction system (akbs and akbt) were detected near the alkylbenzene-degrading operon of rhodococcus sp. dk17. sequence analysis indicates that akbs possesses potential atp-binding and histidine autophosphorylation sites in the n- and c-terminal regions, respectively, and that akbt has a typical response regulator domain. mutant analysis combined with rt-pcr experiments further shows that akbs is required to induce the expression of o-xylene dioxygena ... | 2005 | 15765058 |
| effect of pseudomonas sp. mt5 baths on flavobacterium columnare infection of rainbow trout and on microbial diversity on fish skin and gills. | use of pseudomonas sp. strain mt5 to prevent and treat flavobacterium columnare infection was studied in 2 experiments with fingerling rainbow trout oncorhynchus mykiss. in the first experiment, length heterogeneity analysis of pcr-amplified dna fragments (lh-pcr) was used to assess the effect of antagonistic baths on the microbial diversity of healthy and experimentally infected fish. in the 148 samples studied, no difference was found between bathed and unbathed fish, and 3 fragment lengths we ... | 2005 | 15759801 |
| pulmonary malakoplakia coexistent with tuberculosis of the hilar lymph node mimicking malignancy. | pulmonary malakoplakia is a disease that is difficult to diagnose because its symptoms vary from those mimicking bronchogenic carcinoma to pneumonitis-like tuberculous infections. malakoplakia is an unusual inflammatory condition characterized by the accumulation of macrophages with diagnostic michaelis-gutmann bodies. in this study, an immunocompromised patient with a tumor mass in the upper lobe of the right lung coexisting with hilar node enlargement is presented. a thoracoscopic biopsy revea ... | 2005 | 15753643 |
| characterization of virulence plasmids and serotyping of rhodococcus equi isolates from submaxillary lymph nodes of pigs in hungary. | the plasmid types and serotypes of 164 rhodococcus equi strains obtained from submaxillary lymph nodes of swine from different piggeries in 28 villages and towns located throughout the country were examined. the strains were tested by pcr for the presence of 15- to 17-kda virulence-associated protein antigen (vapa) and 20-kda virulence-associated protein antigen (vapb) genes. plasmid dnas were isolated and analyzed by digestion with restriction endonucleases to estimate size and compare their po ... | 2005 | 15750091 |
| biosynthesis of auxin by the gram-positive phytopathogen rhodococcus fascians is controlled by compounds specific to infected plant tissues. | the role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. the phytopathogen rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. we show here for the first t ... | 2005 | 15746315 |
| assessment in mice of vapa-dna vaccination against rhodococcus equi infection. | there is a need to produce a vaccine against rhodococcus equi pneumonia in foals in which immunity against infection is largely based on a type 1, cell-mediated, immune response. the vapa protein of the virulence plasmid of r. equi is highly immunogenic. to assess the potential of vapa-dna to produce immunity, c57bl/6 and balb/c mice were immunized with a dna vaccine constructed from vapa incorporated into pcdna3.1. the plasmid construct expressed vapa in a cos-7 cell line. intramuscular immuniz ... | 2005 | 15734542 |
| vapb-positive rhodococcus equi infection in an hiv-infected patient in japan. | rhodococcus equi, a bacterium present in soil, is a common cause of pneumonia in foals. this organism has been recognized as an opportunistic pathogen in humans, typically causing infection in immunocompromised hosts such as hiv-infected patients and organ transplant recipients. however, human infection with r. equi has not been reported in japan except in a case involving a laboratory worker. we report the first human case of vapb-positive r. equi pneumonia, which involved an hiv-infected patie ... | 2005 | 15729486 |
| diversity of cultivable actinobacteria in geographically widespread marine sediments. | reports describing actinobacteria isolated from marine environments have been dominated by micromonospora, rhodococcus and streptomyces species. recent culture-independent studies have shown that marine environments contain a high diversity of actinobacterial species that are rarely, if at all, recovered by cultivation-based methods. in this study, it is shown that cultivation-independent methods can be used to guide the application of selective isolation methods. the detection of marine-derived ... | 2005 | 15726286 |
| versatile rhodococcus equi-escherichia coli shuttle vectors. | rhodococcus equi is an intracellular pathogen of macrophages, causing disease in young foals, humans, and sporadically other animals. although r. equi is easy to grow and manipulate, the analysis of virulence is hampered by a lack of molecular tools. this paper describes the development of a number of versatile plasmids for use in r. equi. plasmids prev2 and prev5 use origins of replication derived from the mycobacterium fortuitum plasmids pal5000 and pmf1. these plasmids and their derivatives a ... | 2005 | 15723178 |
| characterization of rhodococcus wratislaviensis strain j3 that degrades 4-nitrocatechol and other nitroaromatic compounds. | the bacterial strain j3 was isolated from soil by selective enrichment on mineral medium containing 4-nitrocatechol as the sole carbon and energy source. this strain was identified as rhodococcus wratislaviensis on the basis of morphology, biochemical, physiological and chemotaxonomic characterization and complete sequencing of the 16s rdna gene. the isolated bacterium could utilize 4-nitrocatechol, 3-nitrophenol and 5-nitroguaiacol as sole carbon and energy sources. stoichiometric release of ni ... | 2005 | 15723176 |
| desulphurization of dibenzothiophene and diesel oils by bacteria. | to study the desulphurization of dibenzothiophene (dbt), a recalcitrant thiophenic component of fossil fuels, by two bacteria namely rhodococcus sp. and arthrobacter sulfureus isolated from oil-contaminated soil/sludge in order to use them for reducing the sulphur content of diesel oil in compliance with environmental regulations. | 2005 | 15715638 |
| immune response to vaccines based upon the vapa protein of the horse pathogen, rhodococcus equi, in a murine model. | rhodococcus equi is a significant pathogen in foals predominantly causing a pyogranulomatous bronchopneumonia. many vaccine candidates have been tested for the prevention of r. equi disease in foals. however, none of these have been developed for widespread commercial use. previous studies have shown that a th1 immune response is imperative for the protection of foals against r. equi disease. in this study a dna and a protein vaccine based upon the well-characterised r. equi virulence-associated ... | 2005 | 15715172 |
| the remarkable rhodococcus erythropolis. | rhodococcus erythropolis cells contain a large set of enzymes that allow them to carry out an enormous number of bioconversions and degradations. oxidations, dehydrogenations, epoxidations, hydrolysis, hydroxylations, dehalogenations and desulfurisations have been reported to be performed by r. erythropolis cells or enzymes. this large array of enzymes fully justifies the prospective application of this bacterium in biotechnology. | 2005 | 15711940 |
| farm characteristics and management practices associated with development of rhodococcus equi pneumonia in foals. | to identify farm characteristics and management practices associated with development of rhodococcus equi pneumonia in foals. | 2005 | 15702691 |
| degradation of 5-nitroguaiacol by soil bacteria of the genus rhodococcus. | two bacterial strains were isolated from forest soil by selective enrichment of the mineral medium containing 4-nitropyrocatechol as the sole carbon and energy source. both strains could utilize 4-nitropyrocatechol and 5-nitroguaiacol. degradation of 5-nitroguaiacol and stoichiometric release of nitrites was measured during its degradation both in growing culture and for resting cells. both strains were unable to degrade other nitroaromatic compounds such as 4-nitroguaiacol, 2-nitrophenol, 3-nit ... | 2004 | 15702555 |
| pyogenic liver abscess due to rhodococcus equi in an immunocompetent host. | a case of pyogenic liver abscess (pla) due to rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. the r. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective organism. | 2005 | 15695730 |
| inhibition of diethyl ether degradation in rhodococcus sp. strain dee5151 by glutaraldehyde and ethyl vinyl ether. | alkyl ether-degrading rhodococcus sp. strain dee5151, isolated from activated sewage sludge, has an activity for the oxidation of a variety of alkyl ethers, aralkyl ethers and dibenzyl ether. the whole cell activity for diethyl ether oxidation was effectively inhibited by 2,3-dihydrofurane, ethyl vinyl ether and glutaraldehyde. glutaraldehyde of less than 30 microm inhibited the activity by a competitive manner with the inhibition constant, k(i) of 7.07+/-1.36 microm. the inhibition type became ... | 2005 | 15686830 |
| a large, mobile pathogenicity island confers plant pathogenicity on streptomyces species. | potato scab is a globally important disease caused by polyphyletic plant pathogenic streptomyces species. streptomyces acidiscabies, streptomyces scabies and streptomyces turgidiscabies possess a conserved biosynthetic pathway for the nitrated dipeptide phytotoxin thaxtomin. these pathogens also possess the nec1 gene which encodes a necrogenic protein that is an independent virulence factor. in this article we describe a large (325-660 kb) pathogenicity island (pai) conserved among these three p ... | 2005 | 15686551 |