Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| prevalence of multidrug resistant gram-positive cocci in a chinese hospital over an 8-year period. | gram-positive cocci are common causes of bloodstream and nosocomial infections, and their multi-drug resistance is an increasingly serious problem. the present study aimed to assess the prevalence of multi-drug-resistant gram-positive cocci in a chinese population. in this retrospective study, data about gram-positive cocci from in-patients (january 2006 and december 2013) at the second hospital of shanxi medical university, taiyuan, china, were reviewed. antimicrobial susceptibility profile of ... | 2015 | 26309609 |
| use of enterococcus faecalis and bacillus atrophaeus as surrogates to establish and maintain laboratory proficiency for concentration of water samples using ultrafiltration. | the u.s. environmental protection agency's (epa) water laboratory alliance (wla) currently uses ultrafiltration (uf) for concentration of biosafety level 3 (bsl-3) agents from large volumes (up to 100-l) of drinking water prior to analysis. most uf procedures require comprehensive training and practice to achieve and maintain proficiency. as a result, there was a critical need to develop quality control (qc) criteria. because select agents are difficult to work with and pose a significant safety ... | 2015 | 26306940 |
| regulatory crosstalk between type i and type ii toxin-antitoxin systems in the human pathogen enterococcus faecalis. | we discovered a chromosomal locus containing 2 toxin-antitoxin modules (tas) with an antisense transcriptional organization in the e. faecalis clinical isolate v583. these tas are homologous to the type i txpa-rata system and the type ii mazef, respectively. we have shown that the putative mazf is toxic for e. coli and triggers rna degradation, and its cognate antitoxin maze counteracts toxicity. the second module, adjacent to mazef, expresses a toxin predicted to belong to the txpa type i famil ... | 2015 | 26305399 |
| correction: enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. | 2015 | 26301715 | |
| unexpected abscess localization of the anterior abdominal wall in an adpkd patient undergoing hemodialysis. | autosomal dominant polycystic kidney disease (adpkd) is one of the most common monogenic disorders and the leading inheritable cause of end-stage renal disease worldwide. cystic and noncystic extrarenal manifestations are correlated with variable clinical presentations so that an inherited disorder is now considered a systemic disease. kidney and liver cystic infections are the most common infectious complications in adpkd patients. furthermore, it is well known that adpkd is commonly associated ... | 2015 | 26301109 |
| transcriptome analysis of enterococcus faecalis in response to alkaline stress. | enterococcus faecalis is the most commonly isolated species from endodontic failure root canals; its persistence in treated root canals has been attributed to its ability to resist high ph stress. the goal of this study was to characterize the e. faecalis transcriptome and to identify candidate genes for response and resistance to alkaline stress using illumina hiseq 2000 sequencing. we found that e. faecalis could survive and form biofilms in a ph 10 environment and that alkaline stress had a g ... | 2015 | 26300863 |
| dentin tubule invasion by enterococcus faecalis under stress conditions ex vivo. | enterococcus faecalis is the species most frequently isolated from failed endodontic treatments because it can survive under stress conditions imposed by root canal treatment. the objective of this study was to determine the ability of e. faecalis to invade dentine tubules under alkaline and energy-starvation stress and to explore the potential mechanisms. roots from single-rooted human teeth were infected with e. faecalis under alkaline and energy-starvation stress conditions. after 4 wk of cul ... | 2015 | 26296719 |
| adhesion potential of intestinal microbes predicted by physico-chemical characterization methods. | bacterial adhesion to epithelial surfaces affects retention time in the human gastro-intestinal tract and therefore significantly contributes to interactions between bacteria and their hosts. bacterial adhesion among other factors is strongly influenced by physico-chemical factors. the accurate quantification of these physico-chemical factors in adhesion is however limited by the available measuring techniques. we evaluated surface charge, interfacial rheology and tensiometry (interfacial tensio ... | 2015 | 26295945 |
| antimicrobial susceptibility patterns of enterococcus faecalis and enterococcus faecium isolated from poultry flocks in germany. | between 2010 and 2011, 145 enterococcus isolates (enterococcus faecalis, n = 127; enterococcus faecium, n = 18) were collected during routine bacteriologic diagnostics from broilers, layers, and fattening turkeys in germany showing various clinical signs. the susceptibility to 24 antimicrobial agents was investigated by broth microdilution test to determine minimum inhibitory concentrations (mics). all e. faecalis isolates (n = 127) were susceptible to the beta-lactam antibiotics ampicillin, amo ... | 2015 | 26292548 |
| effects of ozone and photo-activated disinfection against enterococcus faecalis biofilms in vitro. | the purpose of this study was to compare the antibacterial effects of gaseous ozone (o3) and photo-activated disinfection (pad) methods against enterococcus faecalis (e. faecalis) biofilms. | 2015 | 26289524 |
| biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study. | the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants. | 2017 | 26288622 |
| antibiotic resistance pattern of enterococci isolates from nosocomial infections in a tertiary care hospital in eastern india. | resistance to commonly used antibiotics by enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. the present study was conducted to review the prevalence and identify therapeutic options for nosocomial enterococcal infections in our tertiary care hospital. | 2017 | 26283837 |
| in vitro antimicrobial activity of plants used in traditional medicine in gurage and silti zones, south central ethiopia. | to overcome the escalating problems associated with infectious diseases and drug resistance, discovery of new antimicrobials is crucial. the present study aimed to carry out in vitro antimicrobial analysis of 15 medicinal plant species selected according to their traditional medicinal uses in gurage and silti zones, south central ethiopia. | 2015 | 26283630 |
| functional analysis of a bacitracin resistance determinant located on icecp1, a novel tn916-like element from a conjugative plasmid in clostridium perfringens. | bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. they act by interfering with the biosynthesis of the bacterial cell wall. in this study, we analyzed an avian necrotic enteritis strain of clostridium perfringens that was resistant to bacitracin and produced netb toxin. we identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from enterococcus faecalis. it contained the structural genes bcrabd and a putative reg ... | 2015 | 26282424 |
| [study on the antibacterial activity of four kinds of nano-hydroxyapatite composites against enterococcus faecalis]. | this study aims to compare and determine a kind of nano-hydroxyapatite composite material with good antibacterial efficacy on enterococcusfaecalis (e. faecalis) in vitro. | 2015 | 26281262 |
| cytotoxicity, genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer. | to compare the characteristics of bioceramic endodontic sealer endosequence bc sealer with those of ah plus sealer. | 2015 | 26281002 |
| sterilization of rotary niti instruments within endodontic sponges. | to determine whether the following can be sterilized by autoclaving - endodontic sponges, rotary nickel-titanium (niti) instruments within endodontic sponges, and rotary niti instruments with rubber stoppers. | 2015 | 26280766 |
| antibacterial effects and action modes of asiatic acid. | in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ... | 2015 | 26280399 |
| dna sequence signatures for rapid detection of six target bacterial pathogens using pcr assays. | using streptococcus pyogenes as a model, we previously established a stepwise computational workflow to effectively identify species-specific dna signatures that could be used as pcr primer sets to detect target bacteria with high specificity and sensitivity. in this study, we extended the workflow for the rapid development of pcr assays targeting enterococcus faecalis, enterococcus faecium, clostridium perfringens, clostridium difficile, clostridium tetani, and staphylococcus aureus, which are ... | 2015 | 26279626 |
| cryptogenic mycotic aneurysm of the superior mesenteric artery. | visceral artery aneurysms as a result of arterial degenerative disease are rare (0.1-2%), and superior mesenteric artery (sma) accounts for 3.2% of all reported series. however, mycotic sma aneurysms (smaas) are even rarer, and to the best of our knowledge, this is the first report of cryptogenic mycotic aneurysm of sma by enterococcus faecalis (ef). we report a case of 77-year-old man with 6-week history of supra pubic/left iliac fossa pain, weight loss and fever. the computed tomography demons ... | 2015 | 26276703 |
| effects of a one year reusable contraceptive vaginal ring on vaginal microflora and the risk of vaginal infection: an open-label prospective evaluation. | a contraceptive vaginal ring (cvr) containing nestorone® (nes) and ethinyl estradiol (ee) that is reusable for 1- year (13 cycles) is under development. this study assessed effects of this investigational cvr on the incidence of vaginal infections and change in vaginal microflora. | 2015 | 26267119 |
| structural and functional adaptation of vancomycin resistance vant serine racemases. | vancomycin resistance in gram-positive bacteria results from the replacement of the d-alanyl-d-alanine target of peptidoglycan precursors with d-alanyl-d-lactate or d-alanyl-d-serine (d-ala-d-ser), to which vancomycin has low binding affinity. vant is one of the proteins required for the production of d-ala-d-ser-terminating precursors by converting l-ser to d-ser. vant is composed of two domains, an n-terminal membrane-bound domain, likely involved in l-ser uptake, and a c-terminal cytoplasmic ... | 2015 | 26265719 |
| investigations of the presence of caffeine in the rudawa river, kraków, poland. | caffeine concentration in surface water (rudawa river, kraków, poland) has been being investigated since 2011. the method applied for investigations was developed in 2011, and the first series of measurements of caffeine concentration in surface water began in 2011. caffeine concentration was determined by the gas chromatography-mass spectrometry (gc-ms) method. solid phase extraction (spe) was used to enrich the concentration of caffeine in water samples. as an internal standard, the caffeine i ... | 2015 | 26264791 |
| selective elimination of bacterial faecal indicators in the schmutzdecke of slow sand filtration columns. | slow sand filtration (ssf) is an effective low-tech water treatment method for pathogen and particle removal. yet despite its application for centuries, it has been uncertain to which extent pathogenic microbes are removed by mechanical filtration or due to ecological interactions such as grazing and competition for nutrients. in this study, we quantified the removal of bacterial faecal indicators, escherichia coli and enterococcus faecalis, from secondary effluent of a wastewater treatment plan ... | 2015 | 26264137 |
| an in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (mta) against five oral bacteria. | the purpose of this study was to evaluate the antibacterial ability of three mta (mta-angelus, endocem mta, and proroot mta) against five typical oral bacteria (streptococcus mutans, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus paracasei, and porphyromonas gingivalis). | 2015 | 26263538 |
| loss of antibiotic tolerance in sod-deficient mutants is dependent on the energy source and arginine catabolism in enterococci. | enterococci are naturally tolerant to typically bactericidal cell wall-active antibiotics, meaning that their growth is inhibited but they are not killed even when exposed to a high concentration of the drug. the molecular reasons for this extraordinary tolerance are still incompletely understood. previous work showed that resistance to killing collapsed specifically in mutants affected in superoxide dismutase (sod) activity, arguing that bactericidal antibiotic treatment led to induction of a s ... | 2015 | 26260456 |
| evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on enterococcus faecalis and treponema denticola. | a triple antibiotic mixture (ciprofloxacin; cf, metronidazole; mn, and minocycline; mc) has been used for dental root canal medicaments in pulp regeneration therapy. however, tooth discolorations, cervical root fractures, and inadequate pulp-dentin formation have been reported due to the triple antibiotic regimen. therefore, an antibiotic encapsulated biomimetic nanomatrix gel was developed to minimize the clinical limitations and maximize a natural healing process in root canal infections. in t ... | 2015 | 26257918 |
| effect of starch source (corn, oats or wheat) and concentration on fermentation by equine faecal microbiota in vitro. | the goal was to determine the effect of starch source (corn, oats and wheat) and concentration on: (i) total amylolytic bacteria, group d gram-positive cocci (gpc), lactobacilli and lactate-utilizing bacteria, and (ii) fermentation by equine microbiota. | 2015 | 26255645 |
| a new uv-led device for automatic disinfection of stethoscope membranes. | stethoscopes are widely used by doctors and nurses. poor stethoscope hygiene is a potential source of nosocomial infection. this study aimed to propose an innovative solution, based on the latest advances in ultraviolet (uv) light-emitting diodes (leds), for disinfecting stethoscope membranes automatically and efficiently. | 2015 | 26254501 |
| in vitro activities of tedizolid and linezolid against gram-positive cocci associated with acute bacterial skin and skin structure infections and pneumonia. | tedizolid is a novel, expanded-spectrum oxazolidinone with potent activity against a wide range of gram-positive pathogens. a total of 425 isolates of gram-positive bacteria were obtained consecutively from patients with acute bacterial skin and skin structure infections (absssis) or pneumonia. these isolates included methicillin-susceptible staphylococcus aureus (mssa) (n = 100), methicillin-resistant staphylococcus aureus (mrsa) (n = 100), streptococcus pyogenes (n = 50), streptococcus agalact ... | 2015 | 26248355 |
| composition, cytotoxic and antimicrobial activities of satureja intermedia c.a.mey essential oil. | in this study, the essential oil (eo) constituents from the aerial parts of satureja intermedia c.a.mey were detected by gc and gc/ms. the antimicrobial activity of eo on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. thirty-nine compounds were identified and the main eo constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myr ... | 2015 | 26247936 |
| low level of resistance in enterococci isolated in four hospitals, marseille, france. | enterococci are gram-positive cocci responsible for various infections worldwide, and their prevalence of antibiotic resistance greatly varies worldwide. this study investigates the prevalence of resistance to antibiotics in enterococci from patients admitted in the four university hospitals of marseille between january 2013 and september 2014. two thousand nine hundred seventy-six patients-bacteria couples were identified (2,507 enterococcus faecalis and 469 enterococcus faecium) in the four un ... | 2016 | 26247097 |
| aortico-left atrial fistula: a rare complication of bioprosthetic aortic valve endocarditis secondary to enterococcus faecalis. | paravalvular aortic root abscess with intracardiac fistula formation is an exceedingly rare complication of infective endocarditis. this condition is even more rarely encountered in patients with bioprosthetic valve endocarditis. we report an unusual case of a 68-year-old bosnian female with a bioprosthetic aortic valve, who developed an extensive aortic root abscess, complicated by an aortico-left atrial intracardiac fistula. this case illustrates that a high index of suspicion, prompt diagnosi ... | 2015 | 26246917 |
| phytochemical screening, antioxidant and antibacterial activities of extracts prepared from different tissues of schinus terebinthifolius raddi that occurs in the coast of bahia, brazil. | schinus terebinthifolius is widely used in traditional medicine by brazilian quilombola and indigenous communities for treatment of several diseases. extracts from different tissues are being used to produce creams to treat cervicitis and cervicovaginitis. however, most studies are limited to the assessment of the essential oils and extracts obtained from the leaves. | 2017 | 26246739 |
| antibacterial and residual antimicrobial activities against enterococcus faecalis biofilm: a comparison between edta, chlorhexidine, cetrimide, mtad and qmix. | we compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% edta,2% chlorhexidine,0.2% cetrimide, mtad, and qmix) in a model of enterococcus faecalis biofilm formation. sixty dentin blocks with 3-week e. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. a blank control group was also established. antibacterial activities of the irrigants were evaluated by counting colony forming units. to test resi ... | 2015 | 26245711 |
| antimicrobial susceptibility profile of uropathogens in maluti adventist hospital patients, 2011. | urinary tract infections (utis) are amongst the most common infections encountered globally and are usually treated empirically based on bacterial resistance to antibiotics for a given region. unfortunately in lesotho, no published studies are available to guide doctors in the treatment of utis. treatment protocols for western countries have been adopted, which may not be applicable for this region. | 2015 | 26245603 |
| vancomycin-resistant enterococcal infections: epidemiology, clinical manifestations, and optimal management. | since its discovery in england and france in 1986, vancomycin-resistant enterococcus has increasingly become a major nosocomial pathogen worldwide. enterococci are prolific colonizers, with tremendous genome plasticity and a propensity for persistence in hospital environments, allowing for increased transmission and the dissemination of resistance elements. infections typically present in immunosuppressed patients who have received multiple courses of antibiotics in the past. virulence is variab ... | 2015 | 26244026 |
| synthesis, antibacterial and qsar evaluation of 5-oxo and 5-thio derivatives of 1,4-disubstituted tetrazoles. | a series of 1,4-disubstituted tetrazol-5-ones 3a, 5, 7, 12, 13 and 1,4-disubstituted tetrazol-5-thiones 3b, 9, 10 was synthesized and fully characterized by ir, ms, (1)h nmr and (13)c nmr. the series was evaluated for in vitro antibacterial activity against four gram negative (escherichia coli, proteus mirabilis, klebsiella pneumonia, and pseudomonas aeruginosa) and three gram positive (staphylococcus aureus, enterococcus faecalis, and bacillus subtilis) bacteria. the zone of inhibition was meas ... | 2015 | 26243367 |
| biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples. | the goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients as well as environmental samples in order to determine possible virulence factors and clonal relationship. during a two-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients as well as environmental water samples were tested for the presence of e ... | 2015 | 26242895 |
| a comparison of the antibacterial activity of the two methods of photodynamic therapy (using diode laser 810 nm and led lamp 630 nm) against enterococcus faecalis in extracted human anterior teeth. | failure of endodontic treatment is usually due to an inadequate disinfection of the root canal system. enterococcus faecalis has been widely used as a valuable microbiological marker for in-vitro studies because of its ability to colonize in a biofilm like style in root canals, invading dentinal tubules and resistance to some endodontic treatments. the aim of this study was to investigate the antibacterial effects of two methods of photodynamic therapy using a light emitting diode lamp (led lamp ... | 2016 | 26241781 |
| diversity and dynamics of antibiotic-resistant bacteria in cheese as determined by pcr denaturing gradient gel electrophoresis. | this work reports the composition and succession of tetracycline- and erythromycin-resistant bacterial communities in a model cheese, monitored by polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge). bacterial 16s rrna genes were examined using this technique to detect structural changes in the cheese microbiota over manufacturing and ripening. total bacterial genomic dna, used as a template, was extracted from cultivable bacteria grown without and with tetracycline or e ... | 2015 | 26241491 |
| antibiotic resistance patterns of bacteria causing urinary tract infections in the elderly living in nursing homes versus the elderly living at home: an observational study. | antibiotic resistance is a problem in nursing homes. presumed urinary tract infections (uti) are the most common infection. this study examines urine culture results from elderly patients to see if specific guidelines based on gender or whether the patient resides in a nursing home (nh) are warranted. | 2015 | 26238248 |
| the impact of absorbed photons on antimicrobial photodynamic efficacy. | due to increasing resistance of pathogens toward standard antimicrobial procedures, alternative approaches that are capable of inactivating pathogens are necessary in support of regular modalities. in this instance, the photodynamic inactivation of bacteria (pib) may be a promising alternative. for clinical application of pib it is essential to ensure appropriate comparison of given photosensitizer (ps)-light source systems, which is complicated by distinct absorption and emission characteristic ... | 2015 | 26236292 |
| combined effects of photodynamic therapy and irrigants in disinfection of root canals. | in this study, the combined effects of photodynamic therapy and irrigants in eradicating common endodontic pathogens are evaluated. roots of 80 extracted single rooted teeth are divided into 2 groups (1) mechanical flushing; (2) antibacterial irrigation. after cleaning and shaping, they are inoculated with either (a) streptococcus mutans or (b) enterococcus faecalis and incubated. they are again subdivided and either only irrigated or irrigated and lased. dentin shavings are taken from root cana ... | 2016 | 26235897 |
| additional disinfection with a modified salt solution in a root canal model. | the aim of this study is to investigate the disinfecting properties of a modified salt solution (mss) and calcium hydroxide (ca(oh)2) in a non-direct-contact ex-vivo model. | 2015 | 26234625 |
| infection control in retreatment cases: in vivo antibacterial effects of 2 instrumentation systems. | this in vivo study compared the antibacterial effects of 2 instrumentation systems in root canal-treated teeth with apical periodontitis. | 2015 | 26234543 |
| malignant otitis externa in a healthy non-diabetic patient. | a healthy 60-year-old male was initially treated for external otitis, and subsequently received multiple surgeries including abscess drainage, temporal bone debridement, canaloplasty of the external auditory meatus, and fistula excision and was treated with numerous antibiotics at another hospital over a 1-year period. he was seen at our hospital on february 14, 2014 with a complaint of a non-healing wound behind the left ear and drainage of purulent fluid. he had no history of diabetes mellitus ... | 2016 | 26233245 |
| antimicrobial efficacy of photodynamic therapy, nd:yag laser and qmix solution against enterococcus faecalis biofilm. | lasers have been recommended in final root canal disinfection protocol, however, there is no clear evidence about their efficacy against bacteria in biofilms. the aim of the study was to evaluate and compare the disinfection effect of antimicrobial photodynamic therapy (apdt), nd:yag laser and qmix solution against enterococcus faecalis biofilm. | 2016 | 26232719 |
| identification of vancomycin interaction with enterococcus faecalis within 30 min of interaction time using raman spectroscopy. | vancomycin is an important glycopeptide antibiotic which is used to treat serious infections caused by gram-positive bacteria. however, during the last years, a tremendous rise in vancomycin resistances, especially among enterococci, was reported, making fast diagnostic methods inevitable. in this contribution, we apply raman spectroscopy to systematically characterize vancomycin-enterococci interactions over a time span of 90 min using a sensitive enterococcus faecalis strain and two different ... | 2015 | 26231687 |
| the in vitro effect of irrigants with low surface tension on enterococcus faecalis. | due to the complex anatomy of the root canal system and high surface tension of common root canal irrigants (rci), conducting an investigation on rcis containing surfactants is a priority. the aim of this in vitro study was to verify the antibacterial potential of rci with low surface tension in root canals infected with enterococcus faecalis (e. faecalis). | 2015 | 26229541 |
| the enterococcal cytolysin synthetase has an unanticipated lipid kinase fold. | the enterococcal cytolysin is a virulence factor consisting of two post-translationally modified peptides that synergistically kill human immune cells. both peptides are made by cylm, a member of the lanm lanthipeptide synthetases. cylm catalyzes seven dehydrations of ser and thr residues and three cyclization reactions during the biosynthesis of the cytolysin large subunit. we present here the 2.2 å resolution structure of cylm, the first structural information on a lanm. unexpectedly, the stru ... | 2015 | 26226635 |
| leukocyte presence does not increase microbicidal activity of platelet-rich plasma in vitro. | human platelets are a rich reservoir of molecules that promote regenerative processes and microbicidal activity. this activity might be increased by concentration in platelet-rich plasma (prp) products and modulated by the presence of leukocytes. despite extensive use in clinical procedures, only few studies have investigated prp's real microbicidal potential. therefore, this study aimed at comparing the in vitro microbicidal activity of platelets and leukocyte-enriched prp (l-prp) to pure plate ... | 2015 | 26223356 |
| surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic escherichia coli biofilms against pathogen colonization. | prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates the production of biomass leading to biofouling of the device. since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. crucial to the success of this stra ... | 2015 | 26221844 |
| epidemiological alteration in pathogens found in ground meat in iran: unexpected predominance of vancomycin-resistant enterococcus faecalis. | colonization of the human and animal intestinal tract with potential pathogenic bacteria is correlated with the risk of contamination of food products. the current study analyzed the prevalence of enterococcus faecalis and escherichia coli o157h7 in ground meat in ilam, iran. both index organisms were identified following standard food microbiological methods. for e. faecalis, the susceptibility to vancomycin was tested, and pcr was used to check for the vana gene. e. faecalis was present in all ... | 2015 | 26213680 |
| antimicrobial activity of calcium hydroxide and betamethasone on enterococcus faecalis; an in vitro assessment. | calcium hydroxide (ch) is one of the most common intracanal medications. corticosteroids (cs) are used in endodontics because of their anti-inflammatory activity. this study aimed to evaluate the antimicrobial effect of ch+betamethasone and ch+saline against enterococcus faecalis (e. faecalis) using agar diffusion test and measuring the microbial zone of inhibition (zoi). | 2015 | 26213541 |
| investigation of mechanisms and molecular epidemiology of linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china. | the epidemiological and molecular characteristics of eight linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china (january to july 2014) were investigated. the target site modifications and cfr gene associated with linezolid resistance were not found. results of the epidemiological investigation indicated that linezolid resistance possibly occurred on several independent occasions and was often not related to linezolid administration. | 2016 | 26210761 |
| antibacterial efficacy of pastes against e faecalis in primary root dentin: a confocal microscope study. | management of abscessed primary teeth often present endodontic failure owing to questioned efficiency of dressings or obturating pastes to eliminate enterococcus faecalis, a resistant bacterium, residing in depth of dentinal tubules. the present study evaluates the antimicrobial efficacy of two antibacterial and two obturating pastes in dentinal tubules of primary teeth infected with enterococcus faecalis using viability stain and confocal laser scanning microscope (clsm). | 2015 | 26208070 |
| genome sequences of copper resistant and sensitive enterococcus faecalis strains isolated from copper-fed pigs in denmark. | six strains of enterococcus faecalis (s1, s12, s17, s18, s19 and s32) were isolated from copper fed pigs in denmark. these gram-positive bacteria within the genus enterococcus are able to survive a variety of physical and chemical challenges by the acquisition of diverse genetic elements. the genome of strains s1, s12, s17, s18, s19 and s32 contained 2,615, 2,769, 2,625, 2,804, 2,853 and 2,935 protein-coding genes, with 41, 42, 27, 42, 32 and 44 genes encoding antibiotic and metal resistance, re ... | 2015 | 26203344 |
| probiotic properties of enterococcus strains isolated from traditional naturally fermented cream in china. | the purpose of this study was to evaluate the probiotic properties of enterococcus strains isolated from traditional naturally fermented cream in china. four enterococcus isolates showed high cholesterol removal ability in media were identified as enterococcus durans (klds 6.0930 and 6.0933) and enterococcus faecalis (klds 6.0934 and 6.0935) by 16s rrna and phes gene sequences, respectively, and selected for further evaluation. in order to assess the probiotic potential and safety of these strai ... | 2015 | 26200795 |
| antibacterial and anti-inflammatory activities of an extract, fractions, and compounds isolated from gochnatia pulchra aerial parts. | this paper reports on the in vitro antibacterial and in vivo anti-inflammatory properties of a hydroethanolic extract of the aerial parts of gochnatia pulchra (hegp). it also describes the antibacterial activity of hegp fractions and of the isolated compounds genkwanin, scutellarin, apigenin, and 3,5-o-dicaffeoylquinic acid, as evaluated by a broth microdilution method. while hegp and its fractions did not provide promising results, the isolated compounds exhibited pronounced antibacterial activ ... | 2015 | 26200228 |
| structures of folt in substrate-bound and substrate-released conformations reveal a gating mechanism for ecf transporters. | energy-coupling factor (ecf) transporters are a new family of abc transporters that consist of four subunits, two cytoplasmic atpases ecfa and ecfa' and two transmembrane proteins namely ecfs for substrate-specific binding and ecft for energy coupling. here, we report the 3.2-å resolution crystal structure of the ecfs protein of a folate ecf transporter from enterococcus faecalis-effolt, a close homologue of folt from lactobacillus brevis-lbfolt. structural and biochemical analyses reveal the re ... | 2015 | 26198469 |
| anti-inflammatory, antioxidant and antimicrobial activity characterization and toxicity studies of flowers of "jarilla", a medicinal shrub from argentina. | zuccagnia punctata cav. (fabaceae) is an argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). the chalcones were identified as pigments responsible for the yellow color of the flowers. hydroethanolic extracts were obtained both from fresh flowers and from flowers dried by lyophilization. the extracts were standardized by their phenolic and flavonoids content. their fingerprints by hplc-dad indicated the presence of two chalcones as major compounds (2',4'-dihydrox ... | 2015 | 26197533 |
| in vitro activity of azd0914, a novel bacterial dna gyrase/topoisomerase iv inhibitor, against clinically relevant gram-positive and fastidious gram-negative pathogens. | azd0914, a new spiropyrimidinetrione bacterial dna gyrase inhibitor with a novel mode of inhibition, has activity against bacterial species commonly cultured from patient infection specimens, including fluoroquinolone-resistant isolates. this study assessed the in vitro activity of azd0914 against key gram-positive and fastidious gram-negative clinical isolates collected globally in 2013. azd0914 demonstrated potent activity, with mic90s for azd0914 of 0.25 mg/liter against staphylococcus aureus ... | 2015 | 26195518 |
| protective effect of enterococcus faecalis dapto 512 on the intestinal tract and gut mucosa: milk allergy application. | the allergenicity of β-lactoglobulin (β-lg) was studied by using ussing chamber in a murine model of β-lg allergy supplemented with hydrolysates obtained after fermentation of milk for 48 h at 37 (°)c with enterococcus faecalis dapto 512, isolated from cow milk and identified by 16s rdna sequence analysis. balb/c mice were sensitised intraperitoneally with β-lg. three groups of mice were formed: group 1, composed of naive mice used as control received only nacl; group 2, positive control compose ... | 2015 | 26192744 |
| microbiological toxicity of tilmicosin on human colonic microflora in chemostats. | to evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/ml) for 7 days. prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. colonization resistance of each community was determined by 3 successive daily challenges of salmonella typhimurium ... | 2015 | 26190303 |
| antimicrobial activity and chemometric modelling of south african propolis. | this study reports on the inhibitory and bactericidal properties of 39 south african (sa) propolis samples and three propolis samples from brazil. | 2015 | 26189549 |
| repeated exposures to blue light-activated eosin y enhance inactivation of e. faecalis biofilms, in vitro. | in dentistry, antibacterial photodynamic therapy (a-pdt) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. in the current study, we assessed the ability of eosin y exposed to 3 irradiation protocols at inactivating enterococcus faecalis biofilms, in vitro. | 2015 | 26188278 |
| synthesis and evaluation of isatin-β-thiosemicarbazones as novel agents against antibiotic-resistant gram-positive bacterial species. | methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre) have caused an increasing mortality rate, which means that antibiotic resistance is becoming an important health issue. in the course to screen new agents for resistant bacteria, we identified that a series of isatin-β-thiosemicarbazones (ibts) could inhibit the growth of mrsa and vre. this was the first time that the "familiar" ibt compounds exhibited significant anti gram-positive pathogen activity. ... | 2015 | 26185006 |
| antimicrobial activity of silver and gold in toothpastes: a comparative analysis. | in this study, we compared the antimicrobial activity of identical toothpastes differing only in silver or gold nanoparticles against the activity of one of the common toothpastes containing a chemical active ingredient. we also compared the active concentrations of the toothpastes. | 2015 | 26183852 |
| reduction of enterococcus faecalis in curved root canals after various sizes and tapers of canal preparation. | the aim of this study was to evaluate the reduction of enterococcus faecalis in curved root canals after various sizes and tapers of the canal preparation. | 2017 | 26180416 |
| detection of identical isolates of enterococcus faecalis from the blood and oral mucosa in a patient with infective endocarditis. | the detection of infective endocarditis (ie) of oral origin has been previously discussed. however, there are few reports confirming this infection using molecular biological techniques. we herein describe the case of a 67-year-old man who developed ie. blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to enterococcus faecalis jcm 5803 on sequencing of 16s rrna gene fragments. a random amplification of polymorphic dna (rapd) analysis showed the s ... | 2015 | 26179542 |
| enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. | in this study, we investigated the impact of the cell membrane composition of e. faecalis on its recognition by the host immune system. to this end, we employed an e. faecalis deletion mutant (δbgsa) that does not synthesize the major cell membrane glycolipid diglycosyl-diacylglycerol (dglcdag). proteomic analysis revealed that 13 of a total of 21 upregulated surface-associated proteins of e. faecalis δbgsa were lipoproteins. this led to a total lipoprotein content in the cell membrane of 35.8% ... | 2015 | 26172831 |
| transcription factor rex in regulation of pathophysiology in oral pathogens. | the nad(+) and nadh-sensing transcriptional regulator rex is widely conserved across gram-positive bacteria. rex monitors cellular redox poise and controls the expression of genes/operons involved in diverse pathways including alternative fermentation, oxidative stress responses, and biofilm formation. the oral cavity undergoes frequent and drastic fluctuations in nutrient availability, ph, temperature, oxygen tension, saliva, and shear forces. the oral streptococci are major colonizers of oral ... | 2016 | 26172563 |
| strains of enterococcus faecalis differ in their ability to coexist in biofilms with other root canal bacteria. | to investigate the relationship between protease production and the ability of enterococcus faecalis strains to coexist in biofilms with other bacteria commonly recovered from infected root canals. | 2015 | 26172346 |
| association between triclosan-coated sutures for abdominal wall closure and incisional surgical site infection after open surgery in patients presenting with fecal peritonitis: a randomized clinical trial. | a factor that may influence the incidence of surgical site infections (ssis) is the suture used for closure of the abdominal wall because bacteria may adhere to the suture material. sutures can be coated with antibacterial substances that may reduce the bacterial load in the incision. | 2015 | 26171624 |
| histopathological changes induced in an animal model by potentially pathogenic enterococcus faecalis strains recovered from ready-to-eat food outlets in osun state, nigeria. | enterococci have been implicated as an emerging important cause of several diseases and multiple antibiotic resistance. however, there is little information about the prevalence of pathogenic and/or antibiotic-resistant enterococcus faecalis in ready-to-eat foods in nigeria. here we report the pathogenic potential of three selected antibiotic-resistant e. faecalis strains isolated from food canteens and food outlets with different virulence determinant genes, including efc 12 (with gel (+), esp ... | 2015 | 26170700 |
| l-lactic acid production from glycerol coupled with acetic acid metabolism by enterococcus faecalis without carbon loss. | glycerol is a by-product in the biodiesel production process and considered as one of the prospective carbon sources for microbial fermentation including lactic acid fermentation, which has received considerable interest due to its potential application. enterococcus faecalis isolated in our laboratory produced optically pure l-lactic acid from glycerol in the presence of acetic acid. gas chromatography-mass spectrometry analysis using [1, 2-(13)c2] acetic acid proved that the e. faecalis strain ... | 2016 | 26168904 |
| plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties. | a novel and versatile plasma reactor was used to modify polyethersulphone commercial membranes. the equipment was applied to: i) functionalize the membranes with low-temperature plasmas, ii) deposit a film of poly(methyl methacrylate) (pmma) by plasma enhanced chemical vapor deposition (pecvd) and, iii) deposit silver nanoparticles (snp) by gas flow sputtering. each modification process was performed in the same reactor consecutively, without exposure of the membranes to atmospheric air. scannin ... | 2015 | 26166926 |
| japanese nationwide surveillance in 2011 of antibacterial susceptibility patterns of clinical isolates from complicated urinary tract infection cases. | to investigate antimicrobial susceptibility patterns of various bacterial pathogens isolated from complicated urinary tract infection (uti) cases, the japanese society of chemotherapy, the japanese association of infectious disease, and the japanese society of clinical microbiology conducted the second nationwide surveillance from january to september 2011. with the cooperation of 42 medical institutions throughout japan, 1036 strains belonging to 8 clinically relevant bacterial species were col ... | 2015 | 26166322 |
| the bacterial spectrum and antimicrobial susceptibility in female recurrent urinary tract infection: how different they are from sporadic single episodes? | to analyze and compare the etiological uropathogens and the susceptibility profile findings on urine culture between sporadic cases of community-acquired, uncomplicated urinary tract infection (uti) and recurrent uti cases in women. | 2015 | 26163810 |
| bivalent transition metal complexes of ono donor hydrazone ligand: synthesis, structural characterization and antimicrobial activity. | mononuclear transition metal complexes of mn(ii), co(ii), ni(ii), cu(ii), zn(ii) and cd(ii) with a new hydrazone ligand derived from pyrazine-2-carbohydrazide and 2-hydroxyacetophenone have been synthesized. the isolated complexes were characterized by elemental analysis, spectral and analytical methods including elemental analyses, ir, diffuse reflectance, (1)h-nmr, mass spectra, molar conductance, magnetic moment, esr, xrd, tg and sem analysis. from the elemental analyses data, the stoichiomet ... | 2015 | 26163785 |
| an in vitro comparison of endodontic medicaments propolis and calcium hydroxide alone and in combination with ciprofloxacin and moxifloxacin against enterococcus faecalis. | to evaluate and compare the antimicrobial properties of propolis and calcium hydroxide alone and in combination with ciprofloxacin and moxifloxacin against enterococcus faecalis (e. faecalis). | 2015 | 26162260 |
| antibacterial potential of 2.5% sodium hypochlorite in distinct irrigation protocols on enterococcus faecalis biofilm. | the aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% naocl on enterococcus faecalis biofilm. | 2015 | 26162251 |
| spread of enterococcal surface protein in antibiotic resistant entero-coccus faecium and enterococcus faecalis isolates from urinary tract infections. | enterococci rank among leading cause of nosocomial bacteremia and urinary tract infection in hospital and community acquired infections. several traits that may contribute to enhanced virulence have been identified in enterococci. extracellular surface protein (esp) is a virulence factor that contributes in biofilm formation and resistance to environmental stresses. in this study we aimed to determine occurrence of esp in e. faecium and e. faecalis isolates isolated from urinary tract infections ... | 2015 | 26161154 |
| antimicrobial activity of the essential oil of plectranthus neochilus against cariogenic bacteria. | this work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves of plectranthus neochilus (pn-eo) against a representative panel of oral pathogens. we assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (mic). pn-eo displayed moderate activity against enterococcus faecalis (mic = 250 μg/ml) and streptococcus salivarus (mic = 250 μg/ml), significant activity against streptococcus sob ... | 2015 | 26161115 |
| adaptation to nacl reduces the susceptibility of enterococcus faecalis to melaleuca alternifolia (tea tree) oil. | this study investigated the hypothesis that the salt adaptation response of enterococcus faecalis alters susceptibility to tea tree oil (tto). six e. faecalis isolates were adapted to 6.5 % nacl, and then exposed to tto in phosphate-buffered saline (pbs). one isolate was also exposed to tto in brain heart infusion broth (bhib). the viability of salt-adapted and non-adapted control cells was determined at 0, 45 and 90 min and compared. mics for several antibiotics and tto were also determined by ... | 2015 | 26159776 |
| confocal microscopy evaluation of the effect of irrigants on enterococcus faecalis biofilm: an in vitro study. | the purpose of this study was to evaluate in vitro the effectiveness of two endodontic irrigants and their association against enterococcus faecalis (e. faecalis) by confocal laser scanning microscope (clsm). twenty-four bovine incisors were inoculated in a monoculture of e. faecalis for 21 days. after this period, the teeth were divided into three test groups (n = 5) according to the chemical used. group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine gel (chx), group 3: 2.5% nao ... | 2016 | 26153228 |
| cyclodepsipeptides produced by actinomycetes inhibit cyclic-peptide-mediated quorum sensing in gram-positive bacteria. | cyclic peptides are commonly used as quorum-sensing autoinducers in gram-positive firmicutes bacteria. well-studied examples of such molecules are thiolactone and lactone, used to regulate the expression of a series of virulence genes in the agr system of staphylococcus aureus and the fsr system of enterococcus faecalis, respectively. three cyclodepsipeptides ws9326a, ws9326b and cochinmicin ii/iii were identified as a result of screening actinomycetes culture extracts for activity against the a ... | 2015 | 26149266 |
| antimicrobial resistance and virulence traits in enterococcus strains isolated from dogs and cats. | we investigated presence and prevalence of antibiotic-resistances and other biological characters in enterococci isolated from faeces of healthy dogs and cats because these microorganisms represent important human and veterinary pathogens/opportunists, and a significant burden for healthcare systems. in all samples (n=115) we detected enterococci, with a predominance of enterococcus faecium (42; 36.5%) and enterococcus faecalis (36; 31.3%) species, endowed with virulence traits and multidrug-res ... | 2015 | 26147147 |
| estradiol protects female rats against sepsis induced by enterococcus faecalis improving leukocyte bactericidal activity. | enterococcus faecalis is a gram-positive bacteria described as an important causative agent of sepsis. the contact between host leukocytes and bacteria activates the innate immunity, participating as the first defense mechanism against infection. pro-inflammatory cytokines [including tumor necrosis factor (tnf)-α and interleukin-1β] and nitric oxide (no) are essential to recruitment of leukocytes into the infectious focus as well as their activation for phagocytosis. beyond the bacteria species, ... | 2015 | 26143494 |
| [microbiological characteristics and patterns of resistance in prosthetic joint infections in a referral hospital]. | the prosthetic joint infection is the most feared and catastrophic complication for cause severe physical damage to patients and, generates high economic costs. | 2015 | 26143043 |
| biological activities of morus celtidifolia leaf extracts. | the aims of this research were to examine the antibacterial, cytotoxic and antiradical/antioxidant activities of the organic extracts obtained from the leaves of the medicinal plant morus celtidifolia (family: moraceae). to evaluate its antimicrobial properties, m. celtidifolia was tested against the bacteria of medical importance: bacillus subtilis, staphyloccocus aureus, enterococcus faecalis, escherichia coli, enterobacter cloacae and enterobacter aerogenes. cytotoxic activity was assessed by ... | 2015 | 26142508 |
| enterococcus faecalis inhibits osteoblast differentiation and induces chemokine expression. | enterococcus faecalis is commonly found in root canals of patients with refractory apical periodontitis, often accompanying inflammation and malfunctioning bone regeneration. in this study, we investigated the effect of e. faecalis on osteoblast differentiation and the ability to induce chemokine expression to recruit inflammatory cells. | 2015 | 26141768 |
| enterococcus faecalis promotes osteoclastogenesis and semaphorin 4d expression. | enterococcus faecalis is considered a major bacterial pathogen implicated in endodontic infections and contributes considerably to periapical periodontitis. this study aimed to investigate the potential mechanisms by which e. faecalis accounts for the bone destruction in periapical periodontitis in vitro. osteoclast precursor raw264.7 cells were treated with e. faecalis atcc 29212 and a wild strain of e. faecalis derived clinically from an infected root canal. the results showed that, to some ex ... | 2015 | 26138525 |
| antibacterial activity of chlorhexidine after final irrigation with ethanol: clsm and culture-based method analysis. | this study investigated the effect of 95% ethanol on the antibacterial properties of 2% chlorexidine (chx) over monospecies biofilm (enterococcus faecalis) through a culture-based method, and over multispecies biofilm using confocal laser scanning microscopy (clsm). for monospecies model, e. faecalis biofilm was induced in 40 root canals. the irrigation procedures were: s-saline solution; s/chx-saline solution + chx; e-ethanol; and e/chx-ethanol + chx. microbial sampling was performed at three p ... | 2015 | 26138134 |
| antibacterial monoterpenoid indole alkaloids from alstonia scholaris cultivated in temperate zone. | three new monoterpenoid indole alkaloids, named normavacurine-21-one (1), 5-hydroxy-19, 20-e-alschomine (2), and 5-hydroxy-19, 20-z-alschomine (3), together with thirteen known indole alkaloids (4-16) were isolated from the leaves of alstonia scholaris cultivated in kunming. their structures were elucidated on the basis of extensive spectroscopic analysis, as well as by comparison with the reported spectroscopic data. the leaves of a. scholaris cultivated in kunming, contained picrinine-type alk ... | 2015 | 26136061 |
| molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in belgrade, serbia. | in this study the distribution of species and antimicrobial resistance among vancomycin resistant enterococci (vre) recovered from clinical specimens obtained from five hospitals in belgrade was analyzed. strains were further characterized by pulsed-field gel electrophoresis (pfge). polymerase chain reaction (pcr) was used to investigate the presence of vana and vanb genes and pathogenicity factor genes. identification of 194 vre isolates revealed 154 enterococcus faecium, 21 enterococcus faecal ... | 2015 | 26132835 |
| establishment and characterization of uti and cauti in a mouse model. | urinary tract infections (uti) are highly prevalent, a significant cause of morbidity and are increasingly resistant to treatment with antibiotics. females are disproportionately afflicted by uti: 50% of all women will have a uti in their lifetime. additionally, 20-40% of these women who have an initial uti will suffer a recurrence with some suffering frequent recurrences with serious deterioration in the quality of life, pain and discomfort, disruption of daily activities, increased healthcare ... | 2015 | 26132341 |
| clinical features and treatment of endophthalmitis after cataract surgery. | the aim of this study was to investigate the clinical features and treatment results of endophthalmitis after cataract surgery. five patients with endophthalmitis after phacoemulsification with intraocular lens implantation were enrolled in this study. the pathogenesis, clinical manifestation, and surgical outcomes of 5 patients were compared. three patients were surgically treated with anterior chamber irrigation and vitrectomy with intravitreal injection. the remaining two patients were medica ... | 2015 | 26125869 |
| evaluation of antimicrobial and antifungal efficacy of inter appointment intracanal medicaments against enterococcus and candida albicans: an in vitro study. | microorganisms and their by-products in pulpal and periapical diseases are to be considered as the primary etiological agents of the pulpal necrosis and apical periodontitis. enterococcus faecalis, which is the most common organism isolated from failed root canals, is a gram-positive facultative anaerobe. yeasts can be detected in 7-18% of infected root canals. | 2015 | 26124609 |