Publications

treatment of post-cesarean section endometritis with ampicillin and sulbactam or clindamycin and gentamicin.	seventy-seven patients were prospectively enrolled in a randomized clinical trial to compare two antimicrobial regimens for the treatment of post-cesarean section endometritis. the two groups were not significantly different with respect to age, race, gravidity, parity, hours in labor, cesarean section indication, preoperative or postoperative hemoglobin/hematocrit, pretreatment white blood cell count or pretreatment temperature. pretreatment urine, blood and endometrial cultures were obtained. ...	1993	8277478
anaerobic infection in chronic maxillary sinusitis.	1) ten per cent of cases of chronic maxillary sinusitis in a series of 50 cases treated in the ent department of dayanand medical college, ludhiana, were caused by anaerobic infection. 2) the causative anaerobic micro-organisms encountered were peptostreptococcus and clostridium species. 3) treatment should, therefore, include metronidazole, especially in those cases refractory to conventional antibiotic therapy.	1993	8270303
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.	the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...	1993	8265205
role of suspected periodontopathogens in microbiological monitoring of periodontitis.	periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ...	1993	8260004
[the role anaerobes in dermatitis digitalis et interdigitalis in cattle].	altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species peptococcus asacharolyticus, pc. sacharolyticus, peptostreptococcus anaerobius, bacteroides asacharolyticus and b. melaninogenicus. the remaining isolates were classified as bacteroides, fusobacterium, streptococcus and clostridium. in the above-mentioned strains observat ...	1993	8259638
antimicrobial activity of dv-7751a, a new fluoroquinolone.	we compared the in vitro antibacterial activity of dv-7751a against gram-positive and -negative bacteria with those of quinolones currently available. mics for 90% of the strains tested (mic90s) against clinical isolates of methicillin-susceptible and -resistant staphylococcus aureus and staphylococcus epidermidis were 0.20, 0.39, 0.20, and 0.78 micrograms/ml, respectively. moreover, mic50s for dv-7751a against ofloxacin-resistant methicillin-resistant s. aureus were 4-, 8-, 16-, 32-, and 64-fol ...	1993	8257132
[study of obligate anaerobic bacterial sensitivity to tinidazole and metronidazole (determination of minimal inhibiting concentration--mic)].	comparative susceptibility testing of 428 strains of obligate anaerobic bacteria belonging to genera propionibacterium, arachnia, actinomyces, bacteroides, prevotella, porphyromonas, anaerorhabdus, fibrobacter, fusobacterium, peptostreptococcus and clostridium to metronidazole and tinidazole was performed. the study of the susceptibility of anaerobic bacteria was carried out by the method of serial dilution in brucella agar according to finegold and sutter (1972). strains of b. fragilis species, ...	1993	8249400
description of two morphotypes of peptostreptococcus micros.	peptostreptococcus micros is often isolated from abscesses in several parts of the human body. the oral cavity is considered the natural habitat for the species, which has been implicated as a periodontal pathogen. in plaque samples from periodontitis patients we observed the presence of a rough morphotype of p. micros in addition to the previously recognized smooth morphotype. the rough morphotype has not been described previously. both morphotypes are frequently isolated simultaneously from th ...	1993	8240959
bacterial l-serine dehydratases: a new family of enzymes containing iron-sulfur clusters.	two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. one family, the pyridoxal-5'-phosphate (plp)-dependent l-threonine dehydratases, also use l-serine as substrate. the other, hitherto unrecognized family is the iron-dependent, highly specific bacterial l-serine dehydratases. it has been shown that l-serine dehydratase from the anaerobic bacterium peptostreptococcus asaccharolyticus contai ...	1993	8236444
cleavages of the o- and c-glucosyl bonds of anthrone and 10,10'-bianthrone derivatives by human intestinal bacteria.	a strictly anaerobic bacterium, bifidobacterium sp. sen, capable of hydrolyzing the o-glucosyl of sennosides was isolated from human feces. the bacterium stepwisely hydrolyzed sennoside b to sennidin b through sennidin-8-monoglucoside in pyf medium but not in gam broth. addition of d-glucose to pyf medium resulted in loss of the hydrolyzing activity in culture but addition of d-fructose did not affect the activity. coculture of this bacterium with peptostreptococcus intermedius led to rapid accu ...	1993	8234419
epidural spinal abscess containing gas: mri demonstration.		1993	8232872
[cytotoxicity of anaerobic bacteria isolated from infected root canal].	bacteria in the infected root canal play an important role in the progression of the periapical lesion. the purpose of the present study was to investigate the cytotoxic effects of bacteria isolated from an infected root canal on the periapical lesion. four obligately anaerobic bacteria were isolated and their sonic extracts (se) were prepared. the cytotoxic assay of each se for mc3t3-e1 cell was used with respect to the morphological cell changes and [3h]-thymidine uptake. the following results ...	1993	8228523
trypsin-dependent production of an antibacterial substance by a human peptostreptococcus strain in gnotobiotic rats and in vitro.	an antibacterial substance appeared within 1 day in feces of gnotobiotic rats harboring a human intestinal peptostreptococcus strain. it disappeared when the rat bile-pancreatic duct was ligatured or when the rats ingested a trypsin inhibitor. anaerobic cultures of the peptostreptococcus strain in a medium supplemented with trypsin also exhibited an antibacterial activity, which was also inhibited by the trypsin inhibitor. in vitro the antibacterial substance from both feces and culture medium w ...	1993	8215361
[anaerobic respiratory infection--evaluation of methods of obtaining specimens].	anaerobic cultures were performed on various kinds of specimens obtained from 67 patients with respiratory infections. the infections consisted of 33 cases of pneumonia, 14 cases of lung abscess, 6 cases of pyothorax, 9 cases of chronic lower respiratory tract infection, and 5 cases of infection of pulmonary cyst. specimens included bronchoscopic specimens obtained using a single tube or protected catheter brush (pcb), transtracheal aspirate, percutaneous lung aspirate, aspirate using a suction ...	1994	8207291
in vitro antibacterial activity of fk037, a new parenteral broad-spectrum cephalosporin, against recent clinical isolates in the fields of obstetrics and gynecology.	the antibacterial activity of a new parenteral cephalosporin, fk037 was assessed against recent aerobic and anaerobic strains isolated from patients in the fields of obstetrics and gynecology during the period between january 1992 and june 1993. the mics of fk037 for 90% of the clinical isolates tested were 0.10 microgram/ml for escherichia coli and klebsiella pneumoniae, 0.20 microgram/ml for streptococcus agalactiae, 0.39 microgram/ml for gardnerella vaginalis, 0.78 microgram/ml for staphyloco ...	1994	8205934
[antimicrobial activities of cefuroxime against recent clinical isolates].	antimicrobial activity of cefuroxime axetil (cxm-ax) was compared with those of other cephem antibiotics against clinically isolated strains obtained mainly from outpatients of our center in a period from january to september of 1990 and 1993. minimum inhibitory concentrations were determined and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic80 of cxm were equal to or lower against staphyl ...	1994	8201767
bacterial endocarditis in patients with chronic liver disease.	although patients with cirrhosis have an increased susceptibility for bacterial infections, endocarditis complicating cirrhosis has been reported only infrequently. in this study, our objective was to determine whether, bacterial endocarditis is, in fact, a complicating factor in cirrhosis.	1994	8198106
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.	the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...	1994	8195981
influence of the thymus and the normal microflora on the plasma fibronectin concentration in mice.	the plasma fibronectin (pfn) concentration (cc) of untreated genetically or artificially athymic mice, or treated with tp-4 (thymus hormone sequence analog synthetic preparation) showed no significant difference from their euthymic or untreated controls. in contrast, the pfn cc in mice with different microbiological state showed significant alterations; the highest level occurred in conventional mice and the lower level in germfree mice was increased by bacterial monocontamination. the alternati ...	1993	8184670
antibacterial activity of clavulanate and tazobactam on peptostreptococcus spp.		1994	8182024
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.	bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...	1994	8177022
protein pab, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.	some strains of the anaerobic human commensal and pathogen peptostreptococcus magnus bind human serum albumin (hsa), whereas other strains of this species express protein l, an immunoglobulin light chain-binding surface protein. a novel hsa-binding protein called protein pab was purified in one step from the culture supernatant of an hsa-binding strain of p. magnus by affinity chromatography on hsa-sepharose. the apparent size of the molecular was 47 kda on sds-polyacrylamide gel electrophoresis ...	1994	8163519
relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults.	to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...	1994	8157769
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].	to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...	1994	8151907
[a case of niddm with non-clostridial gas-producing infection in the lower limb--the effects of hyperbaric oxygen therapy].	a 36-year-old male was admitted because of swelling and pain in the lower left limb. he had been diagnosed as having non-insulin dependent diabetes mellitus 4 years previously and had been treated unsuccessfully with insulin. since gas formation was radiographically demonstrated in the soft tissue of the left foot, we treated him with antibiotics and hyperbaric oxygen under the diagnosis of gas gangrene. despite this therapy, the gangrenous lesions progressed. amputation of the left foot was nec ...	1994	8151155
[what kinds of species are in the conjunctiva?].	with suitable culture methods less than 5% of conjunctival fluid specimens remain negative. from approximately 95% of the swab cultures at least 65 bacterial species - although in low numbers - were recovered. among the predominant organisms were gram-positive species like several coagulase-negative staphylococcus spp., propionibacterium spp., corynebacterium spp. and members of the genera peptostreptococcus, streptococcus and actinomyces. among the frequently isolated gram-negative species were ...	1993	8144184
in vitro evaluation of the effectiveness of calcium hydroxide and paramonochlorophenol on anaerobic bacteria from the root canal.	the effectiveness of calcium hydroxide and paramonochlorophenol (pmcp) was tested on anaerobic bacteria isolated from infected root canals, at time intervals 5, 15, 30 and 60 min. the experimental method used was similar to that proposed by the german society of hygiene and microbiology (dghm), with slight modifications. testing revealed that calcium hydroxide was significantly more effective than pmcp against the total number of anaerobes. additionally, it was proved to be quickly and highly ef ...	1993	8143576
determination of 16s ribosomal rna sequences of all members of the genus peptostreptococcus and their phylogenetic position.	a comparative analysis of 16s ribosomal rna sequences of all peptostreptococcus species revealed that most members of the genus peptostreptococcus should be divided into many different genera. the relationship between clostridia and peptostreptococci was analysed to find the phylogenetic position of peptostreptococci.	1994	8132148
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.	the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...	1994	8126176
bacterial colonization and degradation of demineralized dentin matrix in situ.	lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...	1994	8124693
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.	the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...	1994	8123556
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.	peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...	1994	8113169
an unclassified eubacterium taxon in acute dento-alveolar abscess.	the microflora of pus samples aspirated from 50 acute dento-alveolar abscesses was examined. a total of 143 bacterial strains was isolated, consisting predominantly of prevotella spp., alpha-haemolytic streptococcus spp., peptostreptococcus spp. and eubacterium spp. an unclassified asaccharolytic eubacterium taxon was encountered in 17 (34%) of the abscesses. this taxon was found to have a positive association with fusobacterium spp. and a negative association with alpha-haemolytic streptococcus ...	1994	8107059
bacterial and fungal contamination of human fetal liver collected transvaginally for hematopoietic stem cell transplantation.	transplantation of fetal hematopoietic stem cells is a new therapy for fetuses diagnosed in utero with inherited disorders. however, prior to transplantation of fetal stem cells, the cells must be free of microbial contamination. in order to investigate the contamination of human fetal liver tissue, we determined the rate and types of bacterial and fungal contamination of human fetal liver tissue collected transvaginally for use in stem cell transplantation by using the us pharmacopoiea assay. w ...	1993	8101709
management of maxillofacial infections: a review of 50 cases.	maxillofacial infections often place the oral and maxillofacial surgeon in situations where timely decisions have to be made. these decisions can be life-saving. this study reviews 50 infections treated over a 3-year period. the results reveal rapid resolution of the infections by adhering to fundamental principles in their management: recognition of airway compromise, surgical intervention, and the administration of the appropriate antibiotic. a protocol for the management of maxillofacial infe ...	1993	8101564
cytotoxicity versus antibacterial activity of some antiseptics in vitro.	through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...	1993	8100851
an in-vitro study of the comparative viability on different swab types of simulated specimens of bacteria commonly present in oro-facial infections.	an in-vitro study was undertaken to investigate the survival on bacteriological swabs of three potentially pathogenic organisms found in oro-facial infections, by using simulated clinical specimens incubated in the presence or absence of amies transport medium. standard inocula of pure cultures of streptococcus milleri, prevotella melaninogenica and peptostreptococcus anaerobius were soaked on to swabs and plated out on to solid media at various intervals up to 3 days, enabling an estimate of th ...	1993	8099810
iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity.	investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ...	1994	8082806
changes in vaginal flora during pregnancy and association with preterm birth.	a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...	1994	8077737
case report: anaerobic meningitis caused by peptostreptococcus magnus after head and neck surgery.	although anaerobic bacterial meningitis is uncommon, patients subjected to resection of head and neck malignancy appear at special risk. in this article, the authors report on a 72-year-old man in whom meningitis developed after extensive resection of the right sinuses for squamous cell carcinoma; initial treatment consisted of intravenous vancomycin and ceftazidime. intravenous penicillin g was added after the fortuitous early finding of intracellular cocci in wright-giemsa stained cerebral spi ...	1994	8074138
identification of five peptostreptococcus species isolated predominantly from the female genital tract by using the rapid id32a system.	the rapid id32a kit (biomérieux vitek, inc., hazelwood, mo.) was evaluated for its ability to identify peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (virginia polytechnic institute), the current "gold standard" method. a total of 5 peptostreptococcus american type culture collection strains and 95 clinical isolates comprising peptostreptococcus anaerobius, p. asaccharolyticus, p. magnus, p. micros, and p. prevotii isolates were included for ...	1994	8051260
bacteria invading periapical cementum.	the aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. we therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. from two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after a ...	1994	8035155
a quantitative comparison of selected bacteria in human carious dentine by microscopic counts.	the levels of selected cariogenic and obligately anaerobic bacteria have been compared in carious dentine taken from fissures, smooth surfaces, and root surfaces. the numbers of infected dentinal tubules were determined by immunohistological staining using species-specific antisera against selected bacteria. selective localization was observed; mutans streptococci were the predominant bacteria in dentine from fissures and smooth surface coronal caries, but not from root surface caries. the propo ...	1994	8033185
[infectious osteoarthritis of the pubis in sportmen. apropos of 2 cases].	in athletes, osteoarthritis of the symphysis pubis is an uncommon condition that should be promptly differentiated from pubic pain due to microtrauma. the symphysis pubis is infected via the bloodstream, usually by a staphylococcus. pubic pain with fever and severe disability suggests the diagnosis. increased uptake of bone-seeking radionuclides is an early finding. microbiological and histological studies of specimens obtained under radiological guidance confirm the diagnosis. two new cases of ...	1993	8012337
the clinical importance of gram-positive anaerobic cocci isolated at st bartholomew's hospital, london, in 1987.	the clinical importance of the gram-positive anaerobic cocci (gpac) isolated in 1987 at st bartholomew's hospital, london, is assessed. of about 800 anaerobic isolates, 209 (27%) were gpac, of which 67 (32%) were from abscesses and 22 (11%) were in pure growth. four species comprised 77% of the 168 isolates available for study: peptostreptococcus magnus (55 isolates, 33%), p. micros (23, 14%), p. asaccharolyticus (24, 14%) p. asaccharolyticus (24, 14%) and p. anaerobius (27, 16%). different spec ...	1994	8006943
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.	obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...	1994	8004324
distribution of clindamycin in cortical bone during direct local infusion of the canine tibia.	the distribution of clindamycin in tibial cortical bone, administered via direct local infusion with an implantable pump, is described. clindamycin concentrations in cortical bone were measured after 3, 7, and 21 days of intraosseous infusion. the tibia were divided into four quadrants relative to the outflow infusion catheter site located in the medial aspect of the mid-diaphysis. a gradient of 5-30 mm from the infusion site was documented in all four quadrants (proximal lateral, proximal media ...	1994	7996320
[an evaluation of pathogens in patients with bronchopulmonary infection by transtracheal aspiration: december 1978-march 1993].	we performed transtracheal aspiration (tta) in 1165 patients, who were suspected to have bronchopulmonary infection, from december 1978 to march 1993. we isolated pathogens from tta in 806 patients (69.2%). we isolated h. influenzae (62 cases), s. pneumoniae (39 cases) and m. catarrhalis (24 cases) in patients with acute bronchitis, s. pneumoniae (65 cases), alpha-streptococcus sp. (52 cases), h. influenzae (32 cases) and s. aureus (29 cases) in patients with pneumonia or lung abscess and h. inf ...	1994	7996025
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.	this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...	1994	7995646
prophylactic antibiotic treatment in therapeutic or complicated diagnostic ercp: results of a randomized controlled clinical study.	the present study evaluated the effects of prophylactic administration of cefotaxime in patients undergoing therapeutic or complicated diagnostic ercp. one hundred patients were randomized to two groups of 50 patients each. patients in group 1 received an intravenous infusion of 2 g cefotaxime 15 minutes before endoscopy; patients in group 2 did not receive an intravenous antibiotic before ercp (control group). blood samples were drawn for bacteriologic cultures before endoscopy and 5, 15, 30, a ...	1994	7988813
[brain abscess complicating dental caries in children].	brain abscess following dental or periapical infection is rare in childhood. this report describes brain abscesses found in two children with dental caries. case 1.--a 12 year-old boy was admitted because he had suffered from acute meningitis for 3 days. clinical examination showed symptoms of meningitis plus palsy of the right third and fourth cranial nerves and of the left facial nerve, and a defect in the left temporal field. funduscopic examination showed papilledema; ct scan and mri showed ...	1994	7987445
nucleotide sequence of the gene for peptostreptococcal protein l.	a gene bank of peptostreptococcus magnus dna was established using an e. coli host-vector system. western blot analysis identified a clone expressing protein l which bound to the light chain of human immunoglobulins. dna sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kd with a pl of 4.67.	1994	7987012
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.	a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...	1994	7979307
[etiologic factors in acute inflammatory states of the abdominal cavity].	three hundred and two samples of biological material from patients operated because of acute inflammatory states of abdominal cavity were investigated. both aerobic and anaerobic bacteria were investigated. among aerobic flora most frequently occurred enterococci, e. coli and pseudomonas. bacteroides and peptostreptococcus were most frequently occurring anaerobes. mixed flora was seen very often. isolated microorganisms were tested for susceptibility to ciprofloxacin, cefoperazone, cefotaxime, g ...	1994	7967931
rapid diagnosis of anaerobic gram-positive cocci by salt tolerance.	the maximum salt tolerance was 2.5% in cases of peptostreptococcus anaerobius and peptococcus prevotii, 3% in ruminococcus albus, 4% in acidaminococcus fermentans, peptococcus niger and megasphaera elsdenii and 5% in peptococcus magnus. the biochemical tests being largely inactive and time consuming the findings of salt tolerance if combined with those of morphological data provide clue to the rapid identification of gram-positive anaerobic cocci.	1994	7963612
study on treatment of bacterial vaginosis with oral administration of metronidazole or cefdinir.	bacterial vaginosis (bv) is considered to be one of the most common vaginal infections in women. fifteen symptomatic women with bv were enrolled in this study. ten patients with the diagnosis of bv were treated with 10 days oral administration of metronidazole (mtn), 500 mg twice a day, and five patients with cefdinir (cfdn), 300 mg three times a day. in the mtn therapy, the rate of abnormal vaginal discharge subjectively decreased from 100 to 60%, the rate of abnormal vaginal discharges objecti ...	1994	7956461
three-dimensional solution structure of an immunoglobulin light chain-binding domain of protein l. comparison with the igg-binding domains of protein g.	protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. it has affinity for immunoglobulin (ig) through interaction with framework structures in the variable ig light chain domain. the ig-binding activity is located to five homologous repeats called b1-b5 in the n-terminal part of the protein. we have determined the three-dimensional solution structure of the 76 amino acid residue long b1 domain using nmr spectrosc ...	1994	7947810
diagnosis and management of abscesses in the basal ganglia and thalamus: a survey.	a review is made of the current management strategies of abscesses in basal ganglia and thalamus, based on a review of the literature and three of our own cases. clinical picture, aetiology, diagnostic, surgical treatment and outcome are discussed. stereotactic abscess puncture in combination with temporary drainage and rinsing of the abscess cavity in combination with systemic medication of antibiotics has become the management of choice with satisfactory results.	1994	7942190
changes in root canal microbiota during the development of rat periapical lesions.	periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ...	1994	7936717
the functional units of a peptostreptococcal protein l.	protein l is a cell-surface protein from peptostreptococcus which interacts with immunoglobulin kappa light chains. a gene from peptostreptococcus strain 3316 coding for protein l and fragments thereof were expressed in escherichia coli. the peptides were examined for binding to immunoglobulin and serum albumin. the four c units were shown to be responsible for binding to immunoglobulin and the four d units for binding to albumin. this protein l molecule therefore binds to albumin at a site sepa ...	1994	7934898
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.	the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...	1994	7933531
peritonitis following endoscopic polypectomy in a peritoneal dialysis patient: the need for antibiotic prophylaxis.		1994	7927824
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations.	in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...	1994	7924212
study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from lactococcus lactis strains to various strains belonging to human intestinal flora.	the use of genetically modified organisms (gmo) in dairy products requires evaluation of the dna transfer capacity from such organisms among the human intestinal microflora. thus, both in vitro and in vivo [in the digestive tract (dt) of mice] transfer from lactococcus lactis donor strains of the conjugative plasmid pil205 (cmr) and the non-conjugative plasmid pil253 (emr) to: (1) recipient strains isolated from human faecal flora bacteroides sp., bifidobacterium sp., peptostreptococcus sp. (str ...	1994	7921350
advanced periodontitis associated with larsen's syndrome: case report.	larsen's syndrome is a genetic disorder of connective tissue characterized by multiple large-joint dislocations, abnormalities of hands and feet, and anomalous facial features. although findings involving multiple systems have been added to its clinical and pathological entity, oral pathology besides cleft palate has not been reported. we report here advanced periodontitis with severe gingival inflammation in a 29-year-old larsen's syndrome patient. bacterial culture of subgingival plaque identi ...	1995	7899108
major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes.	two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ...	1994	7874880
the gingival immune response to periodontal pathogens in juvenile periodontitis.	a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...	1994	7870467
bacteriological study of bronchoalveolar lavage in immunocompromised hosts.	sixty bronchoalveolar lavage samples collected from lung cancer patients attending the pulmonary tuberculosis and chest diseases unit of the kasturba medical college hospital, manipal; were cultured for both the aerobic and anaerobic organisms. fifty nine samples yielded bacteria in pure culture. pseudomonas aeruginosa (34.8%) was the commonest aerobe, peptostreptococcus was the commonest anaerobe (45.2%) isolated. bacteroides fragilis was isolated in (23.8%) of cases. gentamycin was found to be ...	1994	7868174
peptostreptococcal infection in children.	680 peptostreptococcus sp. (ps) were recovered from 598 (34%) of 1,750 specimens obtained from 554 patients. they included 103 ps asaccharolyticus, 74 ps magnus, 56 ps prevotii, 51 ps micros, 46 ps anaerobius, 11 ps morbilorum, and 10 ps saccharolyticus. most infections were polymicrobial (in 553 instances or 92%) but in 45 (8%), ps were recovered in pure culture. most ps were isolated from abscesses (237), ears (104), peritoneal fluid (95), lung infections (66), bone (30) and sinuses (24). pred ...	1994	7855547
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.	the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...	1994	7853132
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.	a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...	1994	7851086
in vitro activity of du-6859a against anaerobic bacteria.	the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...	1994	7840599
[susceptibility of strict anaerobic bacteria to antibiotics in france: a multicenter study].	during 1992, the in vitro antibiotic susceptibility of 462 anaerobic bacteria was performed in 7 hospitals, by the reference agar dilution method. among the 222 bacteroides fragilis group strains, only one bacteroides fragilis strain was resistant to imipenem and all bêtalactams, even combined with bêtalactamase-inhibitors while metronidazole resistance could not be detected. one major outer membrane protein (probably a porin) was lacking in some of the six amoxicillin-clavulanic acid resistant ...	1994	7824321
on the interaction between single chain fv antibodies and bacterial immunoglobulin-binding proteins.	using four bacterial immunoglobulin-binding proteins, we have analyzed the binding characteristics of a panel of 34 human single chain fv antibodies, expressed in e. coli and with known specificity and sequence. several of the single chain fv antibodies showed affinity for staphylococcal protein a and peptostreptococcal protein l, but not for the streptococcal proteins g or h. the affinity of the binding was higher for protein l (4.5 and 1.4 x 10(9) m-1) than for protein a (7.7 and 6.7 x 10(8) m ...	1994	7822821
[treatment of puerperal endometritis. evaluation of the efficacy and safety of clindamycin + gentamycin vs. penicillin + chloramphenicol + gentamycin].	this was a prospective, single-blind, comparative study in patients with diagnosis of puerperal endometritis, carried out at the loayza hospital in lima, peru. the objective of this study was to evaluate the efficacy and safety of clindamycin and gentamicin in the management of endometritis vs. penicillin, chloramphenicol and gentamicin for 10 days. sixty-five patients were enrolled and 62 were evaluable for efficacy. both treatment groups were comparable in the pre-treatment period in terms of ...	1994	7821833
effect of different combinations of antibiotics on experimental septic peritonitis in rabbits.	this study was undertaken to evaluate the efficacy of different combinations of antimicrobial agents in the treatment of experimental septic peritonitis. two hundred rabbits, divided into ten groups of 20 rabbits each, were used. septic peritonitis was provoked in two stages: treatment of animals by mechanical lavage or antibiotics was performed during the first stage; blood cultures, isolation and identification of aerobic and anaerobic bacteria in the peritoneal cavity were performed during th ...	1994	7819597
role of anaerobic bacteria in chronic otitis media and cholesteatoma.	otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...	1995	7782173
sinusitis and bronchial asthma in children.	sinus disease has been assumed to exist in children with respiratory tract allergies. we consecutively evaluated 375 patients (245 male, 130 female) with childhood onset asthma, between 5 and 15 years of age (mean 7.8 years of age). abnormal waters radiographs were found in 205 patients (54.7%) which included mucosal wall thickening greater than 6 mm (67.3%), complete opacification (22.9%) and air-fluid levels (9.8%). the level of immunoglobulin (ig) e was found to be much higher in non-sinusiti ...	1995	7778440
periodontal findings in spouses. a clinical, radiographic and microbiological study.	clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...	1995	7775676
timing of surgery in patients with infected spinal dermal sinuses: report of two cases.	in the past, if infection was present at the time a spinal dermal sinus was discovered, the sinus opening and cerebrospinal fluid were sterilized with a course of appropriate antibiotic therapy prior to the surgical intervention. the cases of two children with an infected spinal dermal sinus are reported here. one patient developed acute paraplegia during the initial stage of antibiotic therapy, and the second patient, who was admitted only with signs of fever and irritability, experienced after ...	1995	7773970
crystalline surface protein of peptostreptococcus anaerobius.	the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ...	1995	7773401
[the prospects for using the new macrolide antibiotic azithromycin (sumamed) in the combined treatment of periodontitis].	antibacterial activity of sumamed, a macrolide antibiotic, in respect of anaerobic microflora of the oral cavity, such as bacteroides, prevotella, porphyromonas, fusobacterium, peptostreptococcus, str. sanguis and staph. aureus, was assessed. the drug was found to be highly effective in respect of bacteroids (mpc90: 1,4 to 30 mg/mlmg/ml, this permitting the use of sumamed in the treatment of 9 patients with chronic generalized periodontitis in the exacerbation stage. bacteriological and clinical ...	1995	7770869
necrotizing fasciitis of the head and neck with intrathoracic extension.	we present the case history of a white male patient who initially presented in the emergency department with a peritonsillar abscess and subsequently returned several days later with necrotizing cervical fasciitis with intrathoracic extension. the successful treatment, utilizing surgical debridement and hyperbaric oxygen, will be discussed for this disease, which causes up to 50% or greater mortality.	1995	7769648
[bone and joint infections due to unusual pathogens].	between 1989 and 1993, 7 patients--2 men and 5 women from 19 to 70 years of age--with osteomyelitis due to an unusual organism were observed. 3 cases with salmonella typhimurium and one at a time with escherichia coli, peptostreptococcus, propionibacterium acnes and pasteurella multocida. 5 patients were treated operative accompanied with antibiotics and 1 without operation. 1 patient declined the indicated surgical therapy. the outcome were total healing in 3 cases, healing with ankylosis of th ...	1995	7754663
associations of specific bacteria with some endodontic signs and symptoms.	although a wide range of bacterial species has been isolated from infected dental root canals it remains necessary to determine whether any particular group of such bacteria is associated with specific endodontic symptoms and clinical signs. in this study 30 root canals were examined microbiologically; of these, 14 were associated with pain, 20 with tenderness to percussion, 23 presented with wet root canals, seven with swelling, five with purulent exudate and four with a sinus. clinical and mic ...	1994	7751061
adherence of bacteria to intraocular lenses: a prospective study.	the study was designed to investigate the bacterial flora of the operating field during routine cataract surgery and the source of intraocular lens contamination during the surgery.	1995	7742281
surgical sepsis: constancy of antibiotic susceptibility of causative organisms.	it is well documented that antibiotic therapy exerts selective pressure on bacteria. conversion of bacteria from susceptible to resistant to antibiotics has been observed often during antimicrobial therapy. it has been postulated that human intestinal reservoirs facilitate communication of transposons that can transfer resistance determinants among various bacterial species.	1995	7740421
recombinant proteins l and lg. two new tools for purification of murine antibody fragments.	several bacterial cell wall proteins, like proteins a and g, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. in 1988, protein l, a bacterial cell wall protein with ig-binding capacity, was isolated from the anaerobic bacterial species peptostreptococcus magnus. binding data with immunoglobulin fragments suggested that protein l could selectively bind the variable region of human kappa light chains. more recently a recombinan ...	1994	7736533
microbiology of chronic suppurative otitis media in children in surabaya, indonesia.	the aerobic and anaerobic microbiology of 38 children from surabaya, indonesia, who suffered from chronic suppurative otitis media (csom) was studied using strict microbiological methodology. a total of 106 isolates (49 anaerobic and 57 aerobic) were recovered. aerobic organisms alone were isolated from 11 (29%), anaerobic bacteria only in 4 (11%) and mixed aerobic and anaerobic flora were present in 23 (60%). the predominant organisms were peptostreptococcus sp., prevotella sp., bacteroides sp. ...	1995	7729990
intracranial complications of sinusitis in childhood.	complications of sinusitis in children, such as intracranial abscess formation, are uncommon and are often clinically unremarkable in comparison to similar disease processes in adults. between 1983 and 1991, 443 children were admitted to children's national medical center in washington, dc, for treatment of sinusitis. fourteen of these children presented with intracranial extension of the infection and abscess formation. a retrospective review of these patients revealed that the risk of developi ...	1995	7717619
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and peritonitis.	one hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...	1994	7705035
positive and negative associations between bacterial species in dental root canals.	significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ...	1994	7700162
endoscopic treatment of acute frontal sinusitis: indications and limitations.		1995	7675495
multiple ligand interactions for bacterial immunoglobulin-binding proteins on human and murine cells of the hematopoetic lineage.	a group of bacterial ig-binding surface proteins were studied: protein h and m1 are from streptococcus pyogenes and interact with igg, protein l is expressed by peptostreptococcus magnus and shows affinity for ig light chains, whereas protein lg is a chimeric construction combining the binding properties of protein l with the igg-binding activity of protein g from group c and g streptococci. proteins l and h coupled to sepharose were mitogenic for human peripheral blood lymphocytes (pbl) and mou ...	1995	7660068
microbiology of gastrostomy site wound infections in children.	specimens of pus were obtained from gastrostomy site wound infections in 22 children. polymicrobial flora was found in 21 of the 22 wounds. aerobic or facultative bacteria only were isolated in eight (36%) instances and mixed aerobic-anaerobic flora were isolated from the other 14 (64%) wounds. a total of 102 bacterial isolates (57 aerobic and 45 anaerobic) and seven cultures of candida were obtained. the most frequent isolates were escherichia coli (16 isolates). peptostreptococcus spp. (14), e ...	1995	7650731
bacteremia in conjunction with endodontic therapy.	this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...	1995	7641631
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.	when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...	1995	7641626
liver abscesses as initial presentation of crohn's disease.		1995	7639260
[antibacterial activities of cefmenoxime against recent fresh clinical isolates from patients in sinusitis].	in order to evaluate antimicrobial activity of cefmenoxime (cmx), minimum inhibitory concentrations (mics) of cmx and control drugs were determined against clinical isolates from patients of sinusitis that were obtained in our laboratory from october of 1993 to march of 1994. the results are summarized as follows; 1. cmx showed strong antimicrobial activities against streptococcus pneumoniae, haemophilus influenzae and moraxella subgenus branhamella catarrhalis that were 3 major aerobic bacteria ...	1995	7637194
a numerical taxonomic study of the gram-positive anaerobic cocci.	clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...	1995	7629855