Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| Rapid typing of Coxiella burnetii. | Coxiella burnetii has the potential to cause serious disease and is highly prevalent in the environment. Despite this, epidemiological data are sparse and isolate collections are typically small, rare, and difficult to share among laboratories as this pathogen is governed by select agent rules and fastidious to culture. With the advent of whole genome sequencing, some of this knowledge gap has been overcome by the development of genotyping schemes, however many of these methods are cumbersome an ... | 2011 | 22073151 |
| developing a new clinical tool for diagnosing chronic q fever: the coxiella elispot. | definitively establishing a clinical diagnosis of chronic q fever remains challenging, as the diagnostic performance of both conventional serological tests and pcr is limited. given the importance of an early diagnosis of chronic q fever, there is a need for a reliable diagnostic test. we developed an enzyme-linked immunospot assay to measure coxiella burnetii (c. burnetii)-specific t-cell responses (coxiella elispot) to both phase i and phase ii antigens and tested convalescent q fever patients ... | 2011 | 22067057 |
| The ligands of Numb proteins X1 and X2 are specific markers for chronic Q fever. | Q fever is a disease caused by Coxiella burnetii, an obligate intracellular bacterium. Acute Q fever is spontaneously resolutive and is characterized by an efficient immune response. In contrast, chronic Q fever is characterized by dysregulated immune response, as demonstrated by the failure of C. burnetii to induce lymphoproliferation and the lack of granulomas. Recently, it has been demonstrated that when co-expressed in heterologous mammalian cell lines, the ligands of Numb proteins X1 and X2 ... | 2011 | 22066909 |
| the 2007-2010 q fever epidemic in the netherlands: characteristics of notified acute q fever patients and the association with dairy goat farming. | we describe the q fever epidemic in the netherlands with emphasis on the epidemiological characteristics of acute q fever patients and the association with veterinary factors. data from 3,264 notifications for acute q fever in the period from 2007 through 2009 were analysed. the patients most affected were men, smokers and persons aged 40-60 years. pneumonia was the most common clinical presentation (62% in 2007 and 2008). only 3.2% of the patients were working in the agriculture sector and 0.5% ... | 2011 | 22066649 |
| coxiella burnetii vaginal shedding and antibody responses in dairy goat herds in a context of clinical q fever outbreaks. | this study, carried out in three goats herds, was aimed at describing individual responses to q fever infection in an abortive context, focusing on both antibody and shedding levels. seroprevalence of coxiella burnetii (cb) infection and vaginal shedding of 1083 goats were investigated using elisa and real-time qpcr assays, respectively. at the end of the outbreaks, a seroprevalence of 45.0% was found and vaginal shedding appeared massive with levels above 10(4) cb/swab in 42.3% of the whole pop ... | 2011 | 22066517 |
| assessment of vaccination by a phase i coxiella burnetii inactivated vaccine in goat herds in clinical q fever situation. | a study was carried out in order to assess the efficacy of vaccination, using a phase i coxiella burnetii inactivated vaccine (coxevac(®), ceva), within three goat herds experiencing q fever abortions waves. the stratification of the population (n=905) was based on parity and on infection status related to both serological and qpcr vaginal shedding results. control (n=443) and vaccinated (n=462) groups were established in each farm. vaccination was administered to does before mating and to kids ... | 2011 | 22066485 |
| characterization of the gdp-d-mannose biosynthesis pathway in coxiella burnetii: the initial steps for gdp-β-d-virenose biosynthesis. | coxiella burnetii, the etiologic agent of human q fever, is a gram-negative and naturally obligate intracellular bacterium. the o-specific polysaccharide chain (o-ps) of the lipopolysaccharide (lps) of c. burnetii is considered a heteropolymer of the two unusual sugars β-d-virenose and dihydrohydroxystreptose and mannose. we hypothesize that gdp-d-mannose is a metabolic intermediate to gdp-β-d-virenose. gdp-d-mannose is synthesized from fructose-6-phosphate in 3 successive reactions; isomerizati ... | 2011 | 22065988 |
| genomotyping of coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates. | c. burnetii is a gram-negative intracellular y-proteobacteria that causes the zoonotic disease q fever. q fever can manifest as an acute or chronic illness. different typing methods have been previously developed to classify c. burnetii isolates to explore its pathogenicity. here, we report a comprehensive genomotyping method based on the presence or absence of genes using microarrays. the genomotyping method was then tested in 52 isolates obtained from different geographic areas, different host ... | 2011 | 22046248 |
| relevance of the positron emission tomography in the diagnosis of vascular graft infection with coxiella burnetii. | coxiella burnetii, the causative agent of q fever, may cause culture-negative vascular graft infections that can be diagnosed by serology and molecular biology. we present a case of vascular graft infection detected by positron emission tomography (pet) scanner. the presence of c. burnetii was confirmed by high antibody titers and positive polymerase chain reaction specific for c. burnetii. this report emphasizes the relevance of the pet scanner in the diagnosis of infection when used in associa ... | 2011 | 22033287 |
| Coxiella burnetii, the Agent of Q Fever, in Domestic Sheep Flocks from Wyoming, United States. | Abstract Coxiella burnetii, the agent of Q fever, is an intracellular bacterial pathogen. It has a nearly cosmopolitan distribution. We conducted a serological survey of domestic sheep herds for infections with C. burnetii in Wyoming following reports of abortion and open ewes. Based on the serologic evidence, there was no link between reproductive problems and exposure to C. burnetii. However, the overall prevalence of C. burnetii in WY sheep was 7%, which indicates that the agent is present ... | 2011 | 22022809 |
| chronic q fever detection in the netherlands. | 2011 | 22021923 | |
| Bartonella quintana in Ethiopian lice. | Head and clothing lice from Jimma, Ethiopia were investigated for pathogenic bacteria. Genomic DNA from pools of lice was subjected to PCR analysis for Bartonella spp., Borrelia spp. Coxiella burnetii, Rickettsia spp. and Yersinia pestis. All 102 lice pools were negative for the afore mentioned pathogens, with the exception of Bartonella species found among 6 of 65 (9.2%) head lice pools and1 of 33 clothing lice pools. Identification was achieved by sequencing the ribosomal intragenic transcribe ... | 2011 | 22019400 |
| coxiella burnetii in northern fur seal (callorhinus ursinus) placentas from st. paul island, alaska. | abstract the decline in the number of northern fur seal (nfs; callorhinus ursinus) pups on st. paul island, alaska, has led to multidisciplinary research, including investigation into issues of reproductive health and success. given the recent identification of coxiella burnetii in the placenta of two other marine mammal species, nfs placentas were collected from reef rookery on st. paul island, alaska, during the 2010 pupping season, examined histologically, and tested for c. burnetii using p ... | 2011 | 22017469 |
| the coxiella burnetii dot/icm system creates a comfortable home through lysosomal renovation. | understanding the molecular pathogenesis of coxiella burnetii, the causative agent of human q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. the recent development of culture conditions suitable for axenic propagation of c. burnetii has paved the way for the application of a range of genetic techniques to address key questions within the field. recent studies using mutational analysis have revealed that the c. burn ... | 2011 | 22010216 |
| In vitro activity of pentamidine against Tropheryma whipplei. | Pentamidine is a group I intron splice inhibitor used as a chemotherapeutic agent to treat parasitic infections. It was recently found to be efficient intracellularly against Coxiella burnetii, the bacterial agent of Q fever. This in vitro activity was linked to the presence of self-splicing group I introns that disrupt the 23S rRNA of C. burnetii. However, there are several indications that pentamidine may have a wider range of antibacterial activity. The aim of this study was to determine the ... | 2011 | 22005072 |
| Comparison of ELISA and indirect immunofluorescent antibody assay detecting Coxiella burnetii IgM phase II for the diagnosis of acute Q fever. | A commercially available enzyme-linked immunosorbent assay (ELISA) detecting Coxiella burnetii phase II-specific IgM for the diagnosis of acute Q fever was compared with indirect immunofluorescent antibody assay (IFA). IFA is the current reference method for the detection of antibodies against C. burnetii, but has disadvantages because the judgment of fluorescence is subjective and tiring, and the test is expensive and automation is not possible. To examine whether phase II IgM ELISA could be us ... | 2011 | 21997772 |
| shedding and serological patterns of dairy cows following abortions associated with coxiella burnetii dna detection. | to describe both shedding and serological patterns following abortions detected as being associated with coxiella burnetii (cb), 24 cows experiencing an abortion due to cb were followed over a one month period. samples taken on the day of abortion (d0) were followed 3-fold by weekly samplings from day 14 (d14) to d28 after the abortion. milk and vaginal mucus were collected at each weekly sampling and tested using real-time pcr while blood samples were collected 2-fold on d21 and d28 and tested ... | 2011 | 21996545 |
| Notes from the field: Q fever outbreak associated with goat farms--Washington and Montana, 2011. | On April 22, 2011, the Q fever bacterium Coxiella burnetii was detected in a goat placenta collected from a farm in Washington, where 14 of 50 (28%) pregnant does had aborted since January. A county health alert advised health-care providers to ask patients with symptoms compatible with Q fever (e.g., fever, headache, chills, and myalgia) about exposure to goats, and the owners of the farm informed purchasers of their goats that C. burnetii had been detected in their herd. On May 25, the county ... | 2011 | 21993345 |
| development of a method for detecting coxiella burnetii in cheese samples. | coxiella burnetii is the causative agent of q fever, and the main route of infection in humans is inhalation of contaminated aerosols. although oral transmission by contaminated raw milk or dairy products is also a possible route of human infection, there have been few studies investigating the presence of c. burnetii in dairy products. we developed a new method of extracting dna from cheese and detecting c. burnetii dna in cheese samples with a nested pcr assay. the limit of detection was 6.0 × ... | 2011 | 21979453 |
| q fever: a new ocular manifestation. | q fever is a zoonosis caused by coxiella burnetii. ocular manifestations are rare in this infection. we describe the case of a man complaining of an intense retro-orbital headache, fever, arthralgia, and bilateral loss of vision, who showed an anterior uveitis accompanied by exudative bilateral inferior retinal detachment and optic disk edema. at the beginning, a vogt-koyanagi-harada (vkh) syndrome was suspected, but the patient was diagnosed with q fever and treatment with doxycycline was initi ... | 2011 | 21966200 |
| Peripheral blood gene expression in postinfective fatigue syndrome following from three different triggering infections. | Several infections trigger postinfective fatigue syndromes, which share key illness characteristics with each other and with chronic fatigue syndrome (CFS). Previous cross-sectional case-control studies of CFS have suggested that unique gene expression signatures are evident in peripheral blood samples. | 2011 | 21964398 |
| apparent prevalence of antibodies to coxiella burnetii (q fever) in bulk tank milk from dairy herds in southern belgium. | in 2006, a cross-sectional survey was carried out in 206 herds in southern belgium to estimate the proportion of herds with q fever-seropositive bulk tank milk (btm), i.e. herds with an intra-herd seroprevalence >10%. of the tested herds, 119 (57.8%; 95% ci: 51.1-64.4%) were seropositive. most had a low antibody titre. of these herds, 50 were randomly subjected to a real time coxiella burnetii pcr analysis. the proportion of herds excreting coxiella in btm was 30.0% with only 2.0% of herds havin ... | 2011 | 21962829 |
| serological evidence of coxiella burnetii infection in dogs in a regional centre. | objective investigate the seroprevalence of the causative agent of q fever, coxiella burnetii in domestic dogs in the townsville region, north queensland, australia. method blood samples were collected from dogs attending veterinary clinics for routine procedures. results an overall seropositivity of 21.8% (95% confidence interval (ci) 21.6-22.1%) was observed. a retrospective study of samples collected in the same region during 1984-85 was also performed, with an overall seropositivity of 16.0% ... | 2011 | 21933165 |
| culture-negative endocarditis: "whatever remains, however improbable, must be the truth"; the importance of asking the right questions! | the complicated case of a patient with recurrent culture-negative endocarditis with a history of repeated mitral valve replacement is described. investigations disqualified common pathology but serology revealed a diagnosis of q fever endocarditis. the ongoing problematic management of this patient is described, followed by a brief review of the clinical features, investigations and treatment of q fever. | 2009 | 21931578 |
| determination of coxiella burnetii seroprevalence in macropods in australia. | many animal species, including macropods, have the potential to act as atypical reservoirs of the causative agent of q fever, coxiella burnetii. the objective of this study was to determine the seroprevalence of c. burnetii in various macropod species in australia. competitive and indirect elisas were developed for the testing of macropod sera for antibodies to phase ii and i c. burnetii antigens separately. a total of 500 macropod serum samples from selected species sampled in eastern and weste ... | 2011 | 21925808 |
| q fever diagnosis in domestic ruminants: comparison between complement fixation and commercial enzyme-linked immunosorbent assays. | q fever is an important zoonotic disease caused by infection with the bacterium coxiella burnetii. veterinary diagnostic laboratories, including the veterinary laboratories agency (vla) in england and wales, have traditionally relied on the complement fixation test (cft) for serological diagnosis. however, q fever has assumed greater significance in recent years following several large human outbreaks linked to exposure to infected ruminants and it is essential that more reliable tests are intro ... | 2011 | 21908348 |
| Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. | The current study describes the development of a set of 5 multiplex polymerase chain reaction (mPCR) assays for the simultaneous detection of abortive infection agents in bovine fetal tissues, including Brucella spp., Leptospira spp., and Campylobacter fetus (mPCR1); Hammondia heydorni, Neospora caninum, and Toxoplasma gondii (mPCR2); Coxiella burnetii and Chlamydophila psittaci (mPCR3); Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum (mPCR4); and Bovine viral diarrhea virus ... | 2011 | 21908306 |
| prevalence of coxiella burnetii in livestock abortion material using pcr. | 2011 | 21900261 | |
| serological evidence of coxiella burnetii exposure in native marsupials and introduced animals in queensland, australia. | summarythe state of queensland has the highest incidence of q fever in australia. in recent years, there has been an increase in human cases where no contacts with the typical reservoir animals or occupations were reported. the aim of this study was to determine the seroprevalence of coxiella burnetii in australian native animals and introduced animals in northern and southeastern queensland. australian native marsupials sampled included the brushtail possum (trichosurus vulpecula) and common no ... | 2011 | 21892986 |
| Chronic Q fever: relevance of serology. | 2011 | 21890784 | |
| Q fever among culling workers, the Netherlands, 2009-2010. | In 2009, dairy goat farms in the Netherlands were implicated in >2,300 cases of Q fever; in response, 51,820 small ruminants were culled. Among 517 culling workers, despite use of personal protective equipment, 17.5% seroconverted for antibodies to Coxiella burnetii. Vaccination of culling workers could be considered. | 2011 | 21888803 |
| Efficient activation of T cells by human monocyte-derived dendritic cells (HMDCs) pulsed with Coxiella burnetii outer membrane protein Com1 but not by HspB-pulsed HMDCs. | Coxiella burnetii is an obligate intracellular bacterium and the etiologic agent of Q fever; both coxiella outer membrane protein 1 (Com1) and heat shock protein B (HspB) are its major immunodominant antigens. It is not clear whether Com1 and HspB have the ability to mount immune responses against C. burnetii infection. | 2011 | 21888659 |
| Seroprevalence of Coxiella burnetii antibodies in sheep and goats in the Republic of Ireland. | 2011 | 21881024 | |
| [q-fever caused spontaneous abortion]. | q-fever is a zoonotic infection. pregnant women constitute a specific risk group as the infection may cause spontaneous abortion, intrauterine death, growth retardation, oligohydramnios and premature birth. a 39 year-old veterinarian had a spontaneous abortion in pregnancy week seven. during the first weeks of her pregnancy, she handled birth by-products from cows with q-fever. the q-fever titres revealed that she was most likely infected in very early pregnancy. according to the literature, inf ... | 2011 | 21867662 |
| virulence of pathogenic coxiella burnetii strains after growth in the absence of host cells. | coxiella burnetii is a gram-negative bacterium that causes the zoonotic disease q fever. traditionally considered an obligate intracellular agent, the requirement to be grown in tissue culture cells, embryonated eggs, or animal hosts has made it difficult to isolate strains and perform genetic studies on c. burnetii. however, it was recently demonstrated that the attenuated nine mile phase 2 (nm2) c. burnetii strain will grow axenically in acidified citrate cysteine medium (accm) in a 2.5% oxyge ... | 2011 | 21867419 |
| dot/icm type ivb secretion system requirements for coxiella burnetii growth in human macrophages. | central to q fever pathogenesis is replication of the causative agent, coxiella burnetii, within a phagolysosome-like parasitophorous vacuole (pv) in mononuclear phagocytes. c. burnetii modulates pv biogenesis and other host cell functions, such as apoptotic signaling, presumably via the activity of proteins delivered to the host cytosol by a dot/icm type ivb secretion system (t4bss). in this study, we utilized a c. burnetii strain carrying icmd inactivated by the himar1 transposon to investigat ... | 2011 | 21862628 |
| Serological screening for Coxiella burnetii infection and related reproductive performance in high producing dairy cows. | The possible relationship between Coxiella-seropositivity and the reproductive performance of cows during the previous year to the serological screening was examined in three high producing dairy herds. The herds had a history of subfertility (<25% of pregnancies for the total number of AI), abortion (>18% abortions) and a positive polymerase chain reaction test for Coxiella burnetii in the bulk tank milk with an excretion higher than 10(4)Coxiella /ml for all three herds. Antibodies against C. ... | 2011 | 21862091 |
| four-year evaluation of the effect of vaccination against coxiella burnetii on reduction of animal infection and environmental contamination in a naturally infected dairy sheep flock. | vaccination is considered one of the best options for controlling coxiella burnetii infection in livestock. the efficacy of a phase i vaccine was investigated over 4 years in a sheep flock with confirmed c. burnetii infection. shedding was not detected in ewes and yearlings in the last 2 years, but c. burnetii still persisted in the environment. | 2011 | 21856829 |
| Contemporary unconventional clinical use of co-trimoxazole. | Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ... | 2012 | 21851485 |
| [Epidemiological enquiries in two Q fever outbreaks in a community of Baden-Württemberg during 2008 and 2009]. | In 2008 and 2009, two consecutive outbreaks of Q fever in humans were recorded in the district of Freudenstadt, northern Black Forrest, Baden-Württemberg, Germany. In 2008, a total of 41 persons from a single local community fell ill and were found infected with Coxiella burnetii. Although comprehensive diagnostic and epidemiological outbreak investigations were conducted and control measures taken which included vaccination of ruminants at risk in three parts of the affected community, re-occur ... | 2011 | 21848037 |
| coxiella burnetii: a genuinely novel causative agent of pneumonia in the netherlands since may 2007. | 2011 | 21835068 | |
| visits on 'lamb-viewing days' at a sheep farm open to the public was a risk factor for q fever in 2009. | summarybetween february and may 2009, 347 laboratory-confirmed cases of acute q fever were reported in a southern municipal health service region in the netherlands. commercial dairy-goat farms were implicated and control measures were initially targeted there. a preliminary investigation also implicated a non-dairy sheep farm, open to the public on 'lamb-viewing days'. this study tested the association between visiting the non-dairy sheep farm and developing q fever in residents of the region b ... | 2011 | 21835066 |
| what you need to know about q fever. | 2011 | 21834200 | |
| advances in genetic manipulation of obligate intracellular bacterial pathogens. | infections by obligate intracellular bacterial pathogens result in significant morbidity and mortality worldwide. these bacteria include chlamydia spp., which causes millions of cases of sexually transmitted disease and blinding trachoma annually, and members of the +¦-proteobacterial genera anaplasma, ehrlichia, orientia, and rickettsia, agents of serious human illnesses including epidemic typhus. coxiella burnetii, the agent of human q fever, has also been considered a prototypical obligate in ... | 2011 | 21833334 |
| seroprevalence of seven zoonotic infections in nunavik, quebec (canada). | in nunavik, common practices and food habits such as consumption of raw meat and untreated water place the inuit at risk for contracting zoonotic diseases. the aim of this study was to determine the seroprevalence of seven zoonotic infections among the permanent residents of nunavik. the study was conducted in the fall 2004 as part of the nunavik health survey. blood samples from adults aged 18-74ôçâyears (nôçâ=ôçâ917) were collected and analysed for the presence of antibodies against trichinell ... | 2011 | 21824376 |
| prevalence of coxiella burnetii in ticks after a large outbreak of q fever. | q fever has emerged as an important human and veterinary public health problem in the netherlands with major outbreaks in three consecutive years. goat farms are probably the prime source from which coxiella burnetii have spread throughout the environment, infecting people living in the vicinity. coxiella burnetii infection not only spilled over from animal husbandry to humans but could also have spread to neighbouring wildlife and pets forming novel reservoirs and consequently posing another an ... | 2011 | 21824373 |
| epidemiology and clinical features of human infection with coxiella burnetii in denmark during 2006-07. | query (q) fever was virtually unknown in denmark in 2005, when, after the introduction of new sensitive diagnostic methods for coxiella burnetii, an increasing number of positive cattle created concern among people with frequent exposure. this led to a dramatic rise in examinations for q fever among humans in the following 2ôçâyears. the aim of our study was to assess indication for testing and symptoms in individuals with serological signs of infection with c. burnetii. we performed a case-revi ... | 2011 | 21824371 |
| profiling the humoral immune response of acute and chronic q fever by protein microarray. | antigen profiling using comprehensive protein microarrays is a powerful tool for characterizing the humoral immune response to infectious pathogens. coxiella burnetii is a cdc category b bioterrorist infectious agent with worldwide distribution. in order to assess the antibody repertoire of acute and chronic q fever patients we have constructed a protein microarray containing 93% of the proteome of coxiella burnetii, the causative agent of q fever. here we report the profile of the igg and igm s ... | 2011 | 21817167 |
| q fever, spotted fever group, and typhus group rickettsioses among hospitalized febrile patients in northern tanzania. | background. the importance of q fever, spotted fever group rickettsiosis (sfgr), and typhus group rickettsiosis (tgr) as causes of febrile illness in sub-saharan africa is unknown; the putative role of q fever as a human immunodeficiency virus (hiv) coinfection is unclear. methods. we identified febrile inpatients in moshi, tanzania, from september 2007 through august 2008 and collected acute- and convalescent-phase serum samples. a ≥4-fold increase in immunoglobulin (ig) g immunfluorescence ass ... | 2011 | 21810740 |
| undifferentiated febrile illnesses amongst british troops in helmand, afghanistan. | undifferentiated febrile illnesses have been a threat to british expeditionary forces ever since the crusades. the infections responsible were identified during the colonial era, both world wars and smaller conflicts since, but nearly all remain a significant threat today. undiagnosed febrile illnesses have occurred amongst british troops in helmand, afghanistan since 2006 and so a fever study was performed to identify them. | 2011 | 21805764 |
| ribozyme stability, exon skipping and potential role for rna helicase in group i intron splicing by coxiella burnetii. | the 23s rrna gene of coxiella burnetii, the agent of q fever in humans, contains an unusually high number of conserved, selfish genetic elements, including two group i introns, termed cbu.l1917 (l1917) and cbu.l1951 (l1951). to better understand the role that introns play in coxiella's biology, we determined the intrinsic stability (in vitro half-lives) of the encoded ribozymes to be ~15 d for l1917 and ~5 d for l1951, possibly due to differences in their size (551 and 1559 bases, respectively), ... | 2011 | 21803999 |
| investigation of anaplasmosis in yiyuan county, shandong province, china. | to investigate the situation of anaplasmosis in yiyuan county, shandong province. | 2011 | 21803311 |
| proteomics paves the way for q fever diagnostics. | abstract: q fever is a worldwide zoonosis caused by coxiella burnetii. the disease most frequently manifests clinically as a self-limited febrile illness, as pneumonia (acute q fever) or as a chronic illness that presents mainly as infective endocarditis. the extreme infectivity of the bacterium results in large outbreaks, and the recent outbreak in the netherlands underlines its impact on public health. recent studies on the bacterium have included genome sequencing, the investigation of host-b ... | 2011 | 21801463 |
| unraveling persistent host cell infection with coxiella burnetii by quantitative proteomics. | the interaction between the immune system and invading bacteria is sufficient to eradicate microorganisms for the majority of bacterial infections, but suppression of the microbicidal response leads to reactivation or chronic evolution of infections and to bacterial persistence. to identify the cellular pathways affected by bacterial persistence, we applied the ms-driven combined fractional diagonal chromatography (cofradic) proteomics technique for a comparative study of protein expression in t ... | 2011 | 21790200 |
| travel-associated zoonotic bacterial diseases. | purpose of review: bacterial zoonoses are increasingly described in association with travel. some bacterial zoonoses constitute important causes of post-travel illness. we focus on leptospirosis and rickettsiosis - the most common travel-associated bacterial zoonoses. recent findings: leptospirosis is regarded to be the most common zoonotic disease worldwide. in industrialized countries recreational exposures, both domestic and overseas, are increasingly becoming a major source of infection. asy ... | 2011 | 21788890 |
| [laboratory tests in the diagnosis of feral herd infections]. | immunological laboratory tests play an important role in establishing the depth of extent of this or that infectious disease. scheduled immunological monitoring (serological screening) is made to find out whether there are antibodies (abs) against the causative agents of individual feral herd infections. the immunological examination is aimed at detecting abs against the pathogens of infectious diseases of bacterial (ixodes tick-borne borrelioses, tularemia, leptospiroses, human granulocytic ana ... | 2011 | 21786619 |
| detection of coxiella burnetii in complex matrices by using multiplex qpcr during a major q fever outbreak in the netherlands. | q fever, caused by coxiella burnetii, is a zoonosis with a worldwide distribution. a large rural area in the southeast of the netherlands was heavily affected by q fever between 2007 and 2009. this initiated the development of a robust and internally controlled multiplex qpcr assay for detection of c. burnetii dna in veterinary and environmental matrices on suspected q fever affected farms. the qpcr detects three c. burnetii targets (icd, com1 & is1111), and one bacillus thuringiensis internal c ... | 2011 | 21784920 |
| biological terrorism and the allergist's office practice. | during the anthrax outbreak and threat in trenton (2001), our allergy practice experienced increased visits from approximately 50 of our regular patients with symptoms they believed resulted from anthrax exposure. in all cases, their symptoms were caused by a combination of an exacerbation of their underlying allergic disease and anxiety because of possible exposure to anthrax. our objective is to present an orderly approach to the allergist's outpatients presenting with possible exposure to a b ... | 2011 | 21781403 |
| host kinase activity is required for coxiella burnetii parasitophorous vacuole formation. | coxiella burnetii is the etiologic agent of human q fever and targets alveolar phagocytic cells in vivo wherein the pathogen generates a phagolysosome-like parasitophorous vacuole (pv) for replication. c. burnetii displays a prolonged growth cycle, making pv maintenance critical for bacterial survival. previous studies showed that c. burnetii mediates activation of eukaryotic kinases to inhibit cell death, indicating the importance of host signaling during infection. in the current study, we exa ... | 2010 | 21772829 |
| emergence of bovine ehrlichiosis in belgian cattle herds. | bovine ehrlichiosis is a tick-borne rickettsial disease caused by anaplasma phagocytophilum. the disease can also be transmitted to humans. outbreaks in cattle have been described in many european countries. in belgium, infections caused by a. phagocytophilum have been reported in humans and dogs; however, this paper details the first report of ehrlichiosis in cattle herds in belgium. the first case described was in a dairy herd located in eastern belgium. clinical signs included hyperthermia, p ... | 2011 | 21771546 |
| prevalence of coxiella burnetii and rickettsia spp. in ticks and rodents in southern germany. | coxiella burnetii, the causative agent of q fever, and rickettsia spp. are bacterial pathogens that can be transmitted by ticks of the genus dermacentor (i.e., dermacentor marginatus and d. reticulatus). in germany, the occurrence of these ticks is currently limited to few areas. however, due to increasing temperatures, these vectors will likely extend their distribution in the future, and c. burnetii and rickettsia spp. might spread with them. to assess the prospective risk of human infections ... | 2010 | 21771522 |
| human seroreactivity against bartonella species in the democratic republic of congo. | to assess the presence and identity of bartonella species in a pool of human blood samples from drc congo. | 2011 | 21771478 |
| herd-prevalence of coxiella burnetii (q fever) antibodies in dairy cattle farms based on bulk tank milk analysis. | to determine the prevalence of coxiella burnetii (c. burnetii) antibody positive randomly selected dairy herds in southeast iran (kerman). | 2011 | 21771417 |
| coxiella burnetii infection among blood donors during the 2009 q-fever outbreak in the netherlands. | background: in 2007, 2008, and 2009 outbreaks of q-fever occurred in the netherlands with increasing magnitude. the 2009 outbreak with 2354 reported cases is the largest human q-fever outbreak ever recorded. to assess the extent of infection and the safety of donated blood, we tested local blood donations for presence of coxiella burnetii antibodies and dna. study design and methods: starting may 2009, more than 40,000 serum samples were collected from all consenting blood donors in the areas wi ... | 2011 | 21756265 |
| coxiella burnetii culture-negative endocarditis diagnosed by 16s rrna sequencing of heart valve tissue. | 2011 | 21753722 | |
| type ivb secretion systems of legionella and other gram-negative bacteria. | type iv secretion systems (t4sss) play a central role in the pathogenicity of many important pathogens, including agrobacterium tumefaciens, helicobacter pylori, and legionella pneumophila. the t4sss are related to bacterial conjugation systems, and are classified into two subgroups, type iva (t4ass) and type ivb (t4bss). the t4bss, which is closely related to conjugation systems of inci plasmids, was originally found in human pathogen l. pneumophila; pathogenesis by l. pneumophila infection req ... | 2011 | 21743810 |
| potential association between coxiella burnetii seroprevalence and selected risk factors among veterinary students in slovakia. | the present study investigated the prevalence of antibodies to coxiella burnetii and the possible factors predisposing students of veterinary medicine to c. burnetii infections. igg antibodies to phase i and phase ii c. burnetii antigens were determined by elisa. out of 77 students examined, 13 were positive for phase i and 45 were positive for phase ii antibodies. the titres determined were in the range of 1 : 100-1 : 3200. some risk factors may have contributed to the high seroprevalence found ... | 2011 | 21736269 |
| modelling the effect of heterogeneity of shedding on the within herd coxiella burnetii spread and identification of key parameters by sensitivity analysis. | coxiella burnetii is the bacterium responsible for q fever, a worldwide zoonosis. ruminants, especially cattle, are recognized as the most important source of human infections. although a great heterogeneity between shedder cows has been described, no previous studies have determined which features such as shedding route and duration or the quantity of bacteria shed have the strongest impact on the environmental contamination and thus on the zoonotic risk. our objective was to identify key param ... | 2011 | 21723294 |
| epizootiologic investigations of selected abortive agents in free-ranging alpine ibex (capra ibex ibex) in switzerland. | in the early 2000s, several colonies of alpine ibex (capra ibex ibex) in switzerland ceased growing or began to decrease. reproductive problems due to infections with abortive agents might have negatively affected recruitment. we assessed the presence of selected agents of abortion in alpine ibex by serologic, molecular, and culture techniques and evaluated whether infection with these agents might have affected population densities. blood and fecal samples were collected from 651 ibex in 14 col ... | 2011 | 21719818 |
| risks, trust and knowledge: determinants of pregnant women's decisions regarding participation in a future q fever screening and treatment program during a large epidemic in the netherlands. | contracting q fever during pregnancy carries a risk of developing obstetric complications. the aim of this study was to gain insight into pregnant women's decisions regarding participation in a future q fever screening and treatment program. | 2011 | 21717482 |
| coxiella burnetii causing haemophagocytic syndrome: a rare complication of an unusual pathogen. | we describe an unusual presentation of q fever with associated haemophagocytic syndrome, confirmed by bone marrow aspirate, q fever polymerase chain reaction (pcr) and serological testing. clinical recovery was observed after the commencement of doxycycline with normalisation of the patient's full blood count and serum biochemistry. serial monitoring of the q fever serology revealed the subsequent development of sustained high phase 1 igg antibodies, suggestive of chronic q fever. although many ... | 2011 | 21713429 |
| [q fever in petting zoos]. | 2011 | 21702192 | |
| serological evidence of coxiella burnetii infection in beef cattle in queensland. | queensland has the highest incidence of q fever in australia. the aim of this study was to undertake a cross-sectional seroprevalence survey of coxiella burnetii, the causative agent of q fever, in beef cattle in queensland. | 2011 | 21696375 |
| in this year of the veterinarian, we are including a series of papers on the history of veterinary medicine in australia. | disease control during the colonial period in australiaôçâ-àôçâneurological diseases of ruminant livestock in australiaôçâ-àôçâcoxiella burnetii in beef cattle in queenslandôçâ-àôçâdesmotomy for flexural deformity in thoroughbredsôçâ-àôçâacute myeloid leukaemia in a thoroughbred foalôçâ-àôçâgrowth variants of avibacterium paragallinarumôçâ-àôçâvolvulus in a dugong. | 2011 | 21696368 |
| detection of coxiella burnetii, the agent of q fever, in oviducts and uterine flushing media and in genital tract tissues of the non pregnant goat. | the aim of the present study was the detection and quantification of coxiella burnetii dna in the flushing media (oviducts and uterine horns) and genital tract tissues of non pregnant goats from 20 goats chosen at random from 86 goats originating from 56 different breeding herds in south-west france. the serological prevalence rate of c. burnetii in the study population was 70.3%. the dna of c. burnetii was identified using conventional pcr in the flushing media from the oviducts and uterus in 8 ... | 2011 | 21680021 |
| diversity and coexistence of tick-borne pathogens in central germany. | in total, 1000 ixodes ricinus l. ticks were collected from a small recreational forest area in central germany (thuringia) and investigated for the presence of borrelia spp., babesia spp., anaplasma spp., rickettsia spp., coxiella burnetii, and francisella tularensis. overall, 43.6% of the ticks were infected with at least one pathogen. in 8.4% of ticks double infections were detected, and 1.6% harbored more than two pathogens. in this study, we present data on the coexistence of established and ... | 2011 | 21661327 |
| seroprevalence of q fever in naturally infected dairy cattle herds. | coxiella burnetii is the causal agent of q fever, a worldwide spread zoonosis. prevention of c. burnetii shedding in cattle is critical to control the spread of the pathogen between animals, and from animals to humans. vaccination with a phase 1 vaccine has been shown to be effective in preventing shedding when implemented in still susceptible animals, even in infected cattle herds. the identification of these animals (dairy cows and nulliparous females) as targets for vaccination consequently i ... | 2011 | 21645936 |
| coxiella burnetii (q fever) in rattus norvegicus and rattus rattus at livestock farms and urban locations in the netherlands; could rattus spp. represent reservoirs for (re)introduction? | the q fever outbreak in the netherlands in 2007-2010 prompted government interventions to reduce the human incidence by reduction of q fever shedding at dairy goat farms. mandatory hygiene measures were taken, including the control of animal reservoirs. it has been postulated that brown rats, through their commensal nature, form an important factor in the persistent dissemination of endemic circulating coxiella burnetii in nature to domestic animals, livestock and humans. here, the occurrence of ... | 2011 | 21640416 |
| life on the outside: the rescue of coxiella burnetii from its host cell. | for over seven decades, coxiella burnetii, the causative agent of human q fever, has been considered a prototypical obligate intracellular bacterium that relies exclusively on a eukaryotic cell for growth. intracellularly, the organism prospers in an acidified, phagolysosomelike vacuole. c. burnetii has evolved to replicate in this harsh compartment by a mechanism involving acid activation of metabolism. the ∼2 mb genome of c. burnetii is about twice the size of genomes of most obligate intracel ... | 2011 | 21639786 |
| the coxiella burnetii dot/icm system delivers a unique repertoire of type iv effectors into host cells and is required for intracellular replication. | coxiella burnetii, the causative agent of human q fever, is an intracellular pathogen that replicates in an acidified vacuole derived from the host lysosomal network. this pathogen encodes a dot/icm type iv secretion system that delivers bacterial proteins called effectors to the host cytosol. to identify new effector proteins, the functionally analogous legionella pneumophila dot/icm system was used in a genetic screen to identify fragments of c. burnetii genomic dna that when fused to an adeny ... | 2011 | 21637816 |
| follow-up of 686 patients with acute q fever and detection of chronic infection. | background. recent outbreaks in the netherlands allowed for laboratory follow-up of a large series of patients with acute q fever and for evaluation of test algorithms to detect chronic q fever, a condition with considerable morbidity and mortality. methods. for 686 patients with acute q fever, igg antibodies to coxiella burnetii were determined using an immunofluorescence assay at 3, 6, and 12 months of follow-up. polymerase chain reaction (pcr) was performed after 12 months and on earlier seru ... | 2011 | 21628483 |
| vascular complications of q-fever infections. | introduction: coxiella burnetii is a strict intracellular pathogen causing q fever, a worldwide zoonosis with an extensive animal reservoir. chronic q fever infections are frequently associated with cardiovascular complications, mainly endocarditis, and also aortic aneurysms and vascular-graft infection. we present four cases of chronic q fever infections and associated vascular complications, and review the literature to identify major symptoms and assess the prevalence, treatment and outcome i ... | 2011 | 21622013 |
| endocarditis of bovine jugular vein conduit due to q fever. | contegra (medtronic, minneapolis, mn) conduits are routinely used in cases of right ventricular outflow tract reconstruction during congenital heart surgery. we report two cases of q fever endocarditis involving contegra conduits. surgical treatment and distinct aspects of both unusual cases are described. | 2011 | 21620004 |
| frequency of seropositivity for coxiella burnetii immunoglobulins in livestock and abattoir workers in trinidad. | coxiella burnetii is a zoonotic, rickettsial pathogen which causes mild and severe diseases often referred to as q-fever in humans, particularly those occupationally exposed. this study determined the seropositivity for coxiella burnetii igm immunoglobulins using the enzyme immunoassay (eia) in livestock and abattoir workers in trinidad and related to selected personal characteristics to seroprevalence. overall, of the 455 humans whose serum samples were tested, 20 (4.4%) were seropositive for c ... | 2011 | 21617835 |
| proteomic comparison of virulent phase i and avirulent phase ii of coxiella burnetii, the causative agent of q fever. | coxiella burnetii, a category b biological warfare agent, causes multiple outbreaks of the zoonotic disease q fever world-wide, each year. the virulent phase i and avirulent phase ii variants of the nine mile rsa 493 and 439 strains of c. burnetii were propagated in embryonated hen eggs and then purified by centrifugation through renografin gradients. total protein fractions were isolated from each phase and subjected to analysis by one-dimensional electrophoresis plus tandem mass spectrometry. ... | 2011 | 21616182 |
| [q fever in the netherlands: current status, results from veterinary research and expectations of the coming years]. | 2011 | 21614850 | |
| tick-borne bacteria in free-living jaguars (panthera onca) in pantanal, brazil. | abstract tick-borne bacteria were investigated in 10 free-living jaguars and their ticks in the pantanal biome, brazil. jaguar sera were tested by indirect fluorescent antibody assays using rickettsia rickettsii, rickettsia parkeri, rickettsia amblyommii, rickettsia rhipicephali, rickettsia felis, rickettsia bellii, ehrlichia canis, and coxiella burnetii as crude antigens. all 10 jaguar sera reacted (titer ≥64) to at least one rickettsia species; 4 and 3 sera reacted with e. canis and c. burneti ... | 2011 | 21612532 |
| coxiella symbionts in the cayenne tick amblyomma cajennense. | members of the coxiella genus are intracellular bacteria that can infect a variety of animals including humans. a symbiotic coxiella was recently described in amblyomma americanum ticks in the northern hemisphere with no further investigations of other amblyomma species in other geographic regions. these ixodid ticks represent a group of important vectors for human infectious agents. in the present work, we have demonstrated that symbiotic coxiella (scox) are widespread, occurring in south ameri ... | 2011 | 21611689 |
| modelling effectiveness of herd level vaccination against q fever in dairy cattle. | abstract: q fever is a worldwide zoonosis caused by the bacterium coxiella burnetii. the control of this infection in cattle is crucial: infected ruminants can indeed encounter reproductive disorders and represent the most important source of human infection. in the field, vaccination is currently advised in infected herds but the comparative effectiveness of different vaccination protocols has never been explored: the duration of the vaccination programme and the category of animals to be vacci ... | 2011 | 21605376 |
| application of broad-range resequencing array rpm-tei for detection of pathogens in desert dust samples from kuwait and iraq. | a significant percentage of the human population is exposed to high levels of naturally occurring airborne dusts. although the link between airborne particulate inhalation and a variety of respiratory diseases has been long established, little is known about the pathogenic role of the microbial component of the dust. in this study, we applied highly multiplexed pcr and a high-density resequencing microarray (rpm-tei 1.0) to screen samples of fine topsoil particles and airborne dust collected in ... | 2011 | 21571877 |
| a proteomic approach to investigate the differential antigenic profile of two coxiella burnetii strains. | q fever is a widespread zoonosis caused by coxiella burnetii, an obligate intracellular gram-negative bacterium. current diagnostics of q fever is based on serological testing of patient serum. biological distinction among c. burnetii strains has been referred at the genetic level as well as in virulence in animal models of q fever. disclosure of strain specific antigens might show insight into the biology and pathogenesis of this query pathogen, as well as it can provide the literature with pot ... | 2011 | 21565289 |
| q fever in the netherlands: the role of local environmental conditions. | the netherlands is facing a q fever epidemic in which dairy goats are implicated. people living close to an affected farm have an increased risk. however, no human cases were reported around a number of farms with serious q fever problems. to assess the role of local environmental conditions which may add to the transmission or risk of q fever, we gathered datasets on vegetation, land use, soil characteristics, and weather conditions in 5 km areas around infected farms. areas without transmissio ... | 2011 | 21563011 |
| compendium of measures to prevent disease associated with animals in public settings, 2011: national association of state public health veterinarians, inc. | certain venues encourage or permit the public to be in contact with animals, resulting in millions of human-animal interactions each year. these settings include county or state fairs, petting zoos, animal swap meets, pet stores, feed stores, zoologic institutions, circuses, carnivals, educational farms, livestock-birthing exhibits, educational exhibits at schools and child-care facilities, and wildlife photo opportunities. although human-animal contact has many benefits, human health problems a ... | 2011 | 21546893 |
| [q fever as a cause of acute hepatitis accompanying fever.] | q fever is a zoonotic infection caused by coxiella burnetti, which has been previously regarded as an uncommon infectious disease in korea but is sporadically reported recently. common manifestations of acute q fever usually present as influenza-like illness, pneumonia and occasionally hepatitis. herein, we report 4 cases of acute q fever as a cause of acute hepatitis and fever. all patients had fever and non-specific symptoms, and laboratory test showed acute hepatitis. antibody surveys for man ... | 2011 | 21519168 |
| use of a single-tube nested real-time pcr assay to facilitate the early diagnosis of acute q fever. | we have developed a single-tube nested real-time pcr (stn-rt pcr) assay using the repetitive, transposon-like element is1111 as the dna target to facilitate early diagnosis of acute q fever. the use of our proposed diagnostic procedures, including igm detection by serology and the stn-rt pcr assay, significantly increased the diagnostic sensitivity for q fever to 78%, compared to 29% when serology alone was used for subjects providing mainly acute-phase blood samples. | 2011 | 21519134 |
| immunological arousal during acute q fever infection. | physicians often encounter patients who present with a vague clinical syndrome. a wide serological workup is often ordered, which may include tests for coxiella burnetii in endemic areas. often, the results of these tests pose new dilemma, with overlapping positive laboratory assays. the objective of this investigation was to characterise the serological overlap between acute q fever and other infectious and immunological diseases. we retrospectively scanned the files of patients with a positive ... | 2011 | 21509477 |
| evaluation of a diagnostic algorithm for acute q fever in an outbreak setting. | in the peak of the 2009 q fever outbreak in the netherlands, we introduced a diagnostic algorithm for acute q fever with an enzyme-linked immunosorbent assay for immunoglobulin m antibodies to coxiella burnetii phase ii antigens (mii screen) as an initial step. subsequently, an immunofluorescence assay or pcr was performed depending on the mii screen outcome, date of onset of disease, and inpatient or outpatient setting. the impact of mii screen on the number of immunofluorescence assays perform ... | 2011 | 21508172 |
| coxiella burnetii seropositivity is highly stable throughout gestation in lactating high-producing dairy cows. | the aims of this study were to analyse, in high-producing dairy cows, plasma coxiella burnetii antibody titres and seroconversion throughout gestation, along with possible factors affecting such titres. the study was performed on 65 lactating pregnant non-aborting animals in a commercial holstein-friesian dairy herd in northeastern spain. blood samples for antibody determinations were collected on days 40, 90, 120, 150, 180 and 210 of gestation. by general linear model (glm) repeated measures an ... | 2011 | 21507080 |
| the health status of q-fever patients after long-term follow-up. | in the netherlands, from 2007 to 2009, 3,522 q-fever cases were notified from three outbreaks. these are the largest documented outbreaks in the world. previous studies suggest that symptoms can persist for a long period of time, resulting in a reduced quality of life (qol). the aim of this study was to qualify and quantify the health status of q-fever patients after long-term follow-up. | 2011 | 21501483 |
| q fever seroprevalence in metropolitan samples is similar to rural/remote samples in queensland, australia. | q fever is a vaccine preventable disease; however, despite this, high notification numbers are still recorded annually in australia. we investigated the seroprevalence of coxiella burnetii, the q fever agent, in a queensland sample population. notification data (n = 6425) from 1984-2008 were collated, identifying high risk areas of q fever exposure. of these 177 were recorded in children. serum samples were collected from queensland and screened using both an immunoflourescence assay at 1:10 dil ... | 2011 | 21499708 |