Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections. | campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates. | 2007 | 17220252 |
| the orphan response regulator hp1021 of helicobacter pylori regulates transcription of a gene cluster presumably involved in acetone metabolism. | helicobacter pylori is a gastric pathogen for which no nonhuman reservoir is known. in accordance with the tight adaptation to its unique habitat, the human stomach, h. pylori is endowed with a very restricted repertoire of regulatory proteins. nevertheless, the three complete two-component systems of h. pylori were shown to be involved in the regulation of important virulence traits like motility and acid resistance and in the control of metal homeostasis. hp1021 is an orphan response regulator ... | 2007 | 17220217 |
| antibiotic resistance of campylobacter jejuni and campylobacter coli clinical isolates from poland. | we tested 102 campylobacter jejuni and 6 campylobacter coli clinical isolates from poland. all were susceptible to erythromycin. among the tested c. jejuni isolates 55.9% and 13.7% were resistant to ciprofloxacin and tetracycline, respectively. replacement of thr86 with ile in gyra and a plasmid-borne tet(o) gene were the main resistance mechanisms for fluoroquinolones and tetracycline, respectively. | 2007 | 17210776 |
| susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites. | susceptibilities to metabolites of tiamulin (tia) and enrofloxacin (enr) were tested using selected bacteria with previously defined minimal inhibitory concentrations (mic). the tia metabolites tested were: n-deethyl-tiamulin (dtia), 2beta-hydroxy-tiamulin (2beta-htia) and 8alpha-hydroxy-tiamulin (8alpha-htia), and the enr metabolites were: ciprofloxacin (cip) and enrofloxacin n-oxide (enr-n). bacteria, all of porcine origin, were selected as representatives of bacterial infections (staphylococc ... | 2007 | 17194550 |
| the absence of intervening sequences in 23s rrna genes of campylobacter coli isolates from turkeys is a unique attribute of a cluster of related strains which also lack resistance to erythromycin. | certain campylobacter strains harbor a transcribed intervening sequence (ivs) in their 23s rrna genes. following transcription, the ivs is excised, leading to fragmentation of the 23s rrna. the origin and possible functions of the ivs are unknown. furthermore, the distribution of ivs-harboring strains within campylobacter populations is poorly understood. in this study, 104 strains of campylobacter coli from turkeys, representing 27 different multilocus sequence typing-based sequence types (sts) ... | 2007 | 17189444 |
| prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from raw chicken meat and human stools in korea. | prevalence of campylobacter in raw chicken meat and human stools and subsequent antibiotic resistance profiles of the pathogenic isolates obtained from 2000 through 2002 were investigated. campylobacter jejuni and campylobacter coli were isolated from 570 of the 923 raw chicken meat samples collected from traditional markets, large retail stores, or department stores in korea, resulting in the isolation rate of 61.8%. a total of 579 campylobacter isolates were obtained from raw chicken (36.3% fo ... | 2006 | 17186659 |
| campylobacter jejuni multilocus sequence types in humans, northwest england, 2003-2004. | detailed understanding of the epidemiology of campylobacter is increasingly facilitated through use of universal and reproducible techniques for accurate strain differentiation and subtyping. multilocus sequence typing (mlst) enables discriminatory subtyping and grouping of isolate types into genetically related clonal complexes; it also has the advantage of ease of application and repeatability. recent studies suggest that a measure of host association may be distinguishable with this system. w ... | 2006 | 17176563 |
| new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. | the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ... | 2007 | 17173232 |
| distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america). | campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ... | 2007 | 17172457 |
| distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america). | campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ... | 2007 | 17172457 |
| relationship between the severity of hepatitis c virus-related liver disease and the presence of helicobacter species in the liver: a prospective study. | to determine the presence of helicobacter species dna in the liver of chronic hepatitis c (chc) patients with and without cirrhosis as compared to controls, and to identify the bacterial species involved. | 2006 | 17143941 |
| a pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with campylobacter jejuni and campylobacter coli in a high-throughput south african poultry abattoir. | to assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. the samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. the samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. conventional culture-based detection methods of campylobact ... | 2006 | 17137050 |
| study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam. | campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ... | 2006 | 17135525 |
| conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni. | many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ... | 2007 | 17135441 |
| conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni. | many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ... | 2007 | 17135441 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene. | multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ... | 2007 | 17093018 |
| clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene. | multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ... | 2007 | 17093018 |
| characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004. | we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ... | 2006 | 17085893 |
| longitudinal study of finnish campylobacter jejuni and c. coli isolates from humans, using multilocus sequence typing, including comparison with epidemiological data and isolates from poultry and cattle. | we describe a study on the application of multilocus sequence typing for the analysis of campylobacter jejuni and c. coli isolates from human domestically acquired infections in the helsinki-uusimaa area of finland in 1996, 2002, and 2003. in addition, isolates from poultry meat and fecal samples of cattle from the seasonal peak (july to september) in 2003 were included in the study. in total, 361 finnish c. jejuni and c. coli strains were typed. sequence type 45 (st-45) (45%), st-21 (21%), and ... | 2007 | 17085689 |
| cryptic plasmids isolated from campylobacter strains represent multiple, novel incompatibility groups. | three small, cryptic plasmids from the multi-drug-resistant (mdr) campylobacter coli strain rm2228 and one small, cryptic plasmid from the mdr campylobacter jejuni strain rm1170 were sequenced and characterized. pcc2228-1 has some similarity to firmicutes repl family plasmids that replicate via a rolling-circle mechanism. pcc2228-2 is a theta-replicating, iteron-containing plasmid (icp) that is a member of the same incompatibility (inc) group as previously described campylobacter shuttle vectors ... | 2007 | 17064774 |
| natural presence of campylobacter spp. in various internal organs of commercial broiler breeder hens. | campylobacter are known to cause acute bacterial gastroenteritis in humans. poultry products have been implicated as a significant source of these infections. six experiments were performed to determine whether campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. the birds were euth ... | 2006 | 17039849 |
| detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products. | the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ... | 2006 | 17010041 |
| fingerprinting of campylobacter jejuni by using resolution-optimized binary gene targets derived from comparative genome hybridization studies. | the aim of this investigation was to exploit the vast comparative data generated by comparative genome hybridization (cgh) studies of campylobacter jejuni in developing a genotyping method. we examined genes in c. jejuni that exhibit binary status (present or absent between strains) within known plasticity regions, in order to identify a minimal subset of gene targets that provide high-resolution genetic fingerprints. using cgh data from three studies as input, binary gene sets were identified w ... | 2006 | 16997982 |
| recovery methods for detection and quantification of campylobacter depend on meat matrices and bacteriological or pcr tools. | campylobacter is one of the main causes of human foodborne bacterial disease associated with meat consumption in developed countries. therefore, the most effective approach for recovery and detection of campylobacter from meat should be determined. two hundred ninety pork skin and chine samples were inoculated with campylobacter jejuni nctc 11168 and two strains of campylobacter coli. campylobacter cells were then recovered from suspensions and enumerated by direct plating. campylobacter recover ... | 2006 | 16995511 |
| effect of oral administration of butyrivibrio fibrisolvens mdt-1 on experimental enterocolitis in mice. | butyrivibrio fibrisolvens mdt-1, a butyrate-producing strain, was evaluated for use as a probiotic to prevent enterocolitis. oral administration of the mdt-1 strain (10(9) cfu/dose) alleviated the symptoms of colitis (including body weight loss, diarrhea, bloody stool, organic disorder, and mucosal damage) that are induced in mice drinking water that contains 3.0% dextran sulfate sodium. in addition, myeloperoxidase (mpo) activity levels in colonic tissue were reduced, suggesting that mdt-1 miti ... | 2006 | 16988006 |
| intestinal carriage of campylobacter jejuni and campylobacter coli among cattle from south-western norway and comparative genotyping of bovine and human isolates by amplified-fragment length polymorphism. | in a survey conducted in 1999-2001, the carriage of thermotolerant campylobacters in cattle was investigated, and the genetic diversity of c. jejuni within one herd was examined and compared with human isolates. c. jejuni, c. coli and other thermotolerant campylobacter spp. were isolated from intestinal contents from 26%, 3% and 2% of 804 cattle, respectively. the carriage rate was higher in calves (46%) than in adults (29%). twenty-nine c. jejuni isolates from one herd and 31 human isolates fro ... | 2006 | 16987400 |
| mechanisms of transcription activation exerted by gadx and gadw at the gada and gadbc gene promoters of the glutamate-based acid resistance system in escherichia coli. | in escherichia coli the gad system protects the cell from the extreme acid stress encountered during transit through the host stomach. the structural genes gada, gadb, and gadc encode two glutamate decarboxylase isoforms and a glutamate/gamma-aminobutyrate (gaba) antiporter, respectively. glutamate decarboxylation involves both proton consumption and production of gaba, a neutral compound which is finally exported via the gadc antiporter. regulation of gada and gadbc transcription is very comple ... | 2006 | 16980449 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| ganglioside molecular mimicry and its pathological roles in guillain-barré syndrome and related diseases. | 2006 | 16966405 | |
| in vitro and in vivo complementation of the helicobacter pylori arginase mutant using an intergenic chromosomal site. | gene complementation strategies are important in validating the roles of genes in specific phenotypes. complementation systems in helicobacter pylori include shuttle vectors, which transform h. pylori at relatively low frequencies, and chromosomally based approaches. chromosomal complementation strategies are susceptible to polar effects and disruption of other h. pylori genes, leading to unwanted pleiotropic effects. | 2006 | 16961811 |
| detection of thermotolerant campylobacter in ostriches (struthio camelus) in italy. | during the period january 2004 to december 2005 four different ostrich farms were investigated to evaluate the presence of thermotolerant campylobacter. a total of 150 ostriches were examined and all were found to be clinically healthy. campylobacter spp. were isolated from 60/150 cloacal swabs. among the isolates, 48 were identified as campylobacter jejuni whereas the remainder were identified as campylobacter coli. campylobacter lari was not isolated. the ostriches, although apparently in a he ... | 2007 | 16959506 |
| lack of evidence for vertical transmission of campylobacter spp. in chickens. | campylobacter jejuni is a major cause of bacterial food-borne infection in the industrial world. there is evidence that c. jejuni is present in eggs and hatchery fluff, opening the possibility for vertical transmission from hens to progeny. poultry operations in iceland provide an excellent opportunity to study this possibility, since breeding flocks are established solely from eggs imported from grandparent flocks in sweden. this leaves limited opportunity for grandparents and their progeny to ... | 2006 | 16957196 |
| comparative genomic analysis of campylobacter jejuni strains reveals diversity due to genomic elements similar to those present in c. jejuni strain rm1221. | analysis of the complete genomic sequence of campylobacter jejuni strain rm1221 identified four large genomic elements, campylobacter jejuni-integrated elements (cjies), that were absent from c. jejuni strain nctc 11168. to further investigate the genomic diversity of campylobacter, we conducted a comparative genomic analysis from a collection of 67 c. jejuni and 12 campylobacter coli strains isolated from various geographical locations and clinical and veterinary sources. utilizing pcr, we demo ... | 2006 | 16943349 |
| in vitro antimicrobial susceptibility, genetic diversity and prevalence of udp-glucose 4-epimerase (gale) gene in campylobacter coli and campylobacter jejuni from turkey production facilities. | this study evaluated the genetic diversity of multi-drug resistant campylobacter jejuni (n=44) and c. coli (n=30) isolated from 18 turkey houses. antimicrobial resistances to ampicillin, ciprofloxacin and nalidixic acid were higher (p<0.05) in c. coli than in c. jejuni strains. pcr analysis indicated that 82% of total isolates tested, including 91% of c. jejuni and 70% of c. coli tested positive for a 496-bp udp-glucose 4-epimerase (gale) gene. the diversity of isolates was mapped by antibiogram ... | 2006 | 16943028 |
| investigation for possible source(s) of contamination of ready-to-eat meat products with listeria spp. and other pathogens in a meat processing plant in trinidad. | in 2003, there was a recall of three processed (chicken franks, spice ham and turkey ham ready-to-eat (rte) meat products by a large processing plant in trinidad as a result of contamination by listeria monocytogenes. the study was conducted to investigate the possible source(s) of listeria contamination of recalled rte meat products and to determine the prevalence of listeria spp., salmonella spp., escherichia coli and campylobacter spp. in the products and air within the plant. raw and process ... | 2006 | 16943025 |
| synergy between efflux pump cmeabc and modifications in ribosomal proteins l4 and l22 in conferring macrolide resistance in campylobacter jejuni and campylobacter coli. | macrolide-resistant mutants of campylobacter jejuni and campylobacter coli were selected in vitro using erythromycin and tylosin. these mutants exhibited modifications in the ribosomal proteins l4 (g74d) and l22 (insertions at position 86 or 98). a synergy between the cmeabc efflux pump and these modifications in conferring macrolide resistance was observed. | 2006 | 16940070 |
| efficacy of iron chelators on campylobacter concentrations in turkey semen. | campylobacter is a leading bacterial cause of human foodborne infections in the united states. recent studies suggest that the organism is highly prevalent in poultry semen and may contribute to vertical transmission between the breeder hen and offspring. because campylobacter requires iron for its growth and survival, the objective of this study was to determine if the addition of natural and synthetic chelators such as ovotransferrin, desferrioxaime, edta, or 2,2'-dipyridyl could reduce or eli ... | 2006 | 16903479 |
| molecular epidemiologic investigation of campylobacter coli in swine production systems, using multilocus sequence typing. | multilocus sequence typing of 151 campylobacter coli isolates from swine reared in conventional (n = 74) and antimicrobial-free (n = 77) production systems revealed high genotypic diversity. sequence type (st) 1413 was predominant and observed among ciprofloxacin-resistant strains. we identified a c. coli st 828 clonal complex consisting of isolates from both production systems. | 2006 | 16885327 |
| comparative, collaborative, and on-site validation of a taqman pcr method as a tool for certified production of fresh, campylobacter-free chickens. | certified campylobacter-free poultry products have been produced in denmark since 2002, the first example of fresh (unprocessed and nonfrozen) chickens labeled "campylobacter free." this success occurred partly through use of a 4-hour gel-based pcr testing scheme on fecal swabs. in this study, a faster, real-time pcr approach was validated in comparative and collaborative trials, based on recommendations from the nordic system for validation of alternative microbiological methods (nordval). the ... | 2006 | 16885299 |
| unique features of a highly pathogenic campylobacter jejuni strain. | campylobacter jejuni, a major human enteric pathogen, exhibits significant strain-to-strain differences which result in differences in pathogenic potential. c. jejuni 81-176 is a highly virulent strain that exhibits unique pathogenic features and is used by many research laboratories. we have determined the nucleotide sequence of its genome and compared it to the genomes of other sequenced c. jejuni strains. we identified a number of unique genetic features which may confer specific metabolic an ... | 2006 | 16861657 |
| the prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like beta-grasp domains. | ubiquitin (ub)-mediated signaling is one of the hallmarks of all eukaryotes. prokaryotic homologs of ub (this and moad) and e1 ligases have been studied in relation to sulfur incorporation reactions in thiamine and molybdenum/tungsten cofactor biosynthesis. however, there is no evidence for entire protein modification systems with ub-like proteins and deconjugation by deubiquitinating enzymes in prokaryotes. hence, the evolutionary assembly of the eukaryotic ub-signaling apparatus remains unclea ... | 2006 | 16859499 |
| genotyping of campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaa short variable region sequencing. | this investigation describes the development of a generally applicable, bioinformatics-driven, single-nucleotide polymorphism (snp) genotyping assay for the common bacterial gastrointestinal pathogen campylobacter jejuni. snps were identified in silico using the program 'minimum snps', which selects for polymorphisms providing the greatest resolution of bacterial populations based on simpson's index of diversity (d). the high-d snps identified in this study were derived from the combined c. jeju ... | 2006 | 16849726 |
| helicobacter anseris sp. nov. and helicobacter brantae sp. nov., isolated from feces of resident canada geese in the greater boston area. | numbers of nonmigratory canada geese have increased substantially in the past decade, and they have become a nuisance in some urban areas. because of their close contact with humans in parks and areas adjacent to surface waterways, contact with their feces poses a zoonotic risk. a total of 97 geese from 10 separate geographic locales in the greater boston area had their feces sampled for detection of helicobacter spp. identification of helicobacter spp. based on 16s rrna genus-specific helicobac ... | 2006 | 16820454 |
| cladophora (chlorophyta) spp. harbor human bacterial pathogens in nearshore water of lake michigan. | cladophora glomerata, a macrophytic green alga, is commonly found in the great lakes, and significant accumulations occur along shorelines during the summer months. recently, cladophora has been shown to harbor high densities of the fecal indicator bacteria escherichia coli and enterococci. cladophora may also harbor human pathogens; however, until now, no studies to address this question have been performed. in the present study, we determined whether attached cladophora, obtained from the lake ... | 2006 | 16820442 |
| analysis of campylobacter spp. contamination in broilers from the farm to the final meat cuts by using restriction fragment length polymorphism of the polymerase chain reaction products. | we investigated the genotype diversity and dynamics of campylobacter jejuni and campylobacter coli in six commercial broiler farms during rearing and abattoir processing. in total, 223 c. jejuni and 36 c. coli strains isolated (on farm, transportation crates, carcasses after defeathering, and chicken wing meat at the end of the processing line) were subtyped by pcr-rflp based on flagellin (fla typing) gene. eleven (c. jejuni) and four (c. coli) different rflp patterns were found. multiple c. jej ... | 2006 | 16806554 |
| the ability of flagellum-specific proteus vulgaris bacteriophage pv22 to interact with campylobacter jejuni flagella in culture. | there has been a recent resurgent interest in bacteriophage biology. research was initiated to examine campylobacter jejuni-specific bacteriophage in the russian federation to develop alternative control measures for this pathogen. | 2006 | 16803630 |
| agdbnet - antigen sequence database software for bacterial typing. | bacterial typing schemes based on the sequences of genes encoding surface antigens require databases that provide a uniform, curated, and widely accepted nomenclature of the variants identified. due to the differences in typing schemes, imposed by the diversity of genes targeted, creating these databases has typically required the writing of one-off code to link the database to a web interface. here we describe agdbnet, widely applicable web database software that facilitates simultaneous blast ... | 2006 | 16790057 |
| atypical helicobacter canadensis strains associated with swine. | forty-two helicobacter isolates were isolated from swine feces in the netherlands and denmark. all 12 isolates sequenced (16s rrna gene) formed a robust clade with helicobacter canadensis ( approximately 99% similarity). species-specific pcr indicated that all of the isolates were h. canadensis isolates. although the appearance of the porcine isolates was similar to the appearance of h. canadensis, only one of these isolates was able to hydrolyze indoxyl acetate, a cardinal characteristic of thi ... | 2006 | 16751570 |
| temporal prevalence of antimicrobial resistance in campylobacter spp. from beef cattle in alberta feedlots. | antimicrobial resistance (amr) was temporally assessed in campylobacters isolated from beef cattle (7,738 fecal samples from 2,622 animals) in four commercial feedlots in alberta. all calves were administered chlortetracycline and oxytetracycline in feed, and a majority of the animals (93%) were injected with long-acting oxytetracycline upon arrival at the feedlot. fecal samples from individual animals were collected upon arrival (i.e., entry sample), 69 days (standard deviation [sd] = 3 days) a ... | 2006 | 16751519 |
| glycosylation of b-type flagellin of pseudomonas aeruginosa: structural and genetic basis. | the flagellin of pseudomonas aeruginosa can be classified into two major types-a-type or b-type-which can be distinguished on the basis of molecular weight and reactivity with type-specific antisera. flagellin from the a-type strain pak was shown to be glycosylated with a heterogeneous o-linked glycan attached to thr189 and ser260. here we show that b-type flagellin from strain pao1 is also posttranslationally modified with an excess mass of up to 700 da, which cannot be explained through phosph ... | 2006 | 16740946 |
| macrolide resistance in campylobacter jejuni and campylobacter coli. | infection with campylobacter jejuni is now considered to be the most common cause of acute bacterial gastroenteritis in humans worldwide. it occurs more frequently than infections caused by salmonella species, shigella species, or escherichia coli o157:h7. although c. jejuni is also recognized for its association with serious post-infection neurological complications, most patients with c. jejuni infections have a self-limited illness. nevertheless, a substantial proportion of these infections a ... | 2006 | 16735431 |
| high genetic variation in the multidrug transporter cmeb gene in campylobacter jejuni and campylobacter coli. | this study was conducted to examine the genetic variation occurring in the cmeb gene encoding the transporter component of the cmeabc efflux pump. | 2006 | 16735430 |
| occurrence of selected foodborne pathogens on poultry and poultry giblets from small retail processing operations in trinidad. | we conducted a study to determine quantitatively and qualitatively the presence of campylobacter spp., escherichia coli, staphylococci, total coliforms, total aerobic bacteria, and salmonella on broiler carcasses from selected small retail processors in trinidad. we used standard media and procedures for detection and quantification. all carcass and weep samples were positive for aerobic bacteria, e. coli, total coliforms, and staphylococci. significant differences in the mean counts of aerobic ... | 2006 | 16715810 |
| effect of direct culture versus selective enrichment on the isolation of thermophilic campylobacter from feces of mature cattle at harvest. | campylobacterjejuni and campylobacter coli are leading bacterial causes of human gastroenteritis in the united states and other industrialized nations. these organisms frequently colonize avian hosts, including commercial poultry, but are also found in the gastrointestinal tract of other warm-blooded animals, including swine, sheep, and cattle. this study investigated the effect of direct culture versus selective enrichment on the isolation of thermophilic campylobacter from the colon of 610 cat ... | 2006 | 16715799 |
| flagellin-independent regulation of chemokine host defense in campylobacter jejuni-infected intestinal epithelium. | campylobacter jejuni is a leading cause of bacterial food-borne diarrheal disease throughout the world and the most frequent antecedent of autoimmune neuropathy guillain-barré syndrome. while infection is associated with immune memory, little is known regarding the role of the epithelium in targeting dendritic cells (dc) for initiating the appropriate adaptive immune response to c. jejuni. the objective of this study was to define the role for the intestinal epithelium in the induction of the ad ... | 2006 | 16714574 |
| iha from an escherichia coli urinary tract infection outbreak clonal group a strain is expressed in vivo in the mouse urinary tract and functions as a catecholate siderophore receptor. | virulence factors of pathogenic escherichia coli belonging to a recently emerged and disseminated clonal group associated with urinary tract infection (uti), provisionally designated clonal group a (cga), have not been experimentally investigated. we used a mouse model of ascending uti with cga member strain ucb34 in order to identify genes of cga that contribute to uti. iha was identified to be expressed by strain ucb34 in the mouse kidney using selective capture of transcribed sequences. iha f ... | 2006 | 16714573 |
| genotyping of campylobacter coli and c. jejuni from retail chicken meat and humans with campylobacteriosis in slovenia and bosnia and herzegovina. | thermotolerant campylobacter jejuni and c. coli are one of the major causes of bacterial foodborne enteric infection. consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. the aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant campylobacter isolates according to species (c. coli, c. jejuni), isolation source (retail chicken meat and human clinica ... | 2006 | 16712997 |
| a large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, france, 2000. | a large waterborne outbreak of infection that occurred during august 2000 in a local community in france was investigated initially via a rapid survey of visits to local physicians. a retrospective cohort study was then conducted on a random cluster sample of residents. of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). those who had drunk tap water h ... | 2006 | 16700706 |
| epidemiology and genetic characterization of shigella flexneri strains isolated from three paediatric populations in egypt (2000-2004). | ninety-seven isolates of shigella flexneri from children seeking medical care from three sites in egypt were characterized. overall, 46.4% of children (median age 17 months) were febrile or reported blood in their stools, 25.8% were dehydrated and 16.5% were admitted to hospital. serotypes 2a (37.1%), 1b (18.6%), 1c (17.5%), and 6 (15.5%) comprised over 88.7% of the total isolates. we observed marked resistance to ampicillin (87.6%), tetracycline (84.5%) and trimethoprim-sulfamethoxazole (63.9%) ... | 2006 | 16690004 |
| purification and spectroscopic characterization of ctb, a group iii truncated hemoglobin implicated in oxygen metabolism in the food-borne pathogen campylobacter jejuni. | campylobacter jejuni is a food-borne bacterial pathogen that possesses two distinct hemoglobins, encoded by the ctb and cgb genes. the former codes for a truncated hemoglobin (ctb) in group iii, an assemblage of uncharacterized globins in diverse clinically and technologically significant bacteria. here, we show that ctb purifies as a monomeric, predominantly oxygenated species. optical spectra of ferric, ferrous, o(2)- and co-bound forms resemble those of other hemoglobins. however, resonance r ... | 2006 | 16681372 |
| characterization of the arsrs regulon of helicobacter pylori, involved in acid adaptation. | the human gastric pathogen helicobacter pylori is extremely well adapted to the highly acidic conditions encountered in the stomach. the pronounced acid resistance of h. pylori relies mainly on the ammonia-producing enzyme urease; however, urease-independent mechanisms are likely to contribute to acid adaptation. acid-responsive gene regulation is mediated at least in part by the arsrs two-component system consisting of the essential ompr-like response regulator arsr and the nonessential cognate ... | 2006 | 16672598 |
| effect of conventional and organic production practices on the prevalence and antimicrobial resistance of campylobacter spp. in poultry. | intestinal tracts of broilers and turkeys from 10 conventional broiler farms and 10 conventional turkey farms, where antimicrobials were routinely used, and from 5 organic broiler farms and 5 organic turkey farms, where antimicrobials had never been used, were collected and cultured for campylobacter species. a total of 694 campylobacter isolates from the conventional and organic poultry operations were tested for antimicrobial resistance to nine antimicrobial agents by the agar dilution method. ... | 2006 | 16672508 |
| dissemination of fluoroquinolone-resistant campylobacter spp. within an integrated commercial poultry production system. | while characterizing the intestinal bacterial community of broiler chickens, we detected epsilon-proteobacterial dna in the ilea of 3-day-old commercial broiler chicks (j. lu, u. idris, b. harmon, c. hofacre, j. j. maurer, and m. d. lee, appl. environ. microbiol. 69:6816-6824, 2003). the sequences exhibited high levels of similarity to campylobacter jejuni and campylobacter coli sequences, suggesting that chickens can carry campylobacter at a very young age. campylobacter sp. was detected by pcr ... | 2006 | 16672489 |
| recurrent outbreaks of cryptosporidiosis associated with calves among students at an educational farm programme, minnesota, 2003. | enteric illness outbreaks among middle-/high-school students in consecutive semesters of an educational farm programme were investigated with retrospective cohort studies. during the first outbreak, 31/92 (34%) interviewed students were ill. risk factors included participating in animal science class (rr 8.1, 95% ci 1.2-55.2) and contact with calves (rr 4.2, 95% ci 1.1-16.2). stool samples from seven students and two calves yielded cryptosporidium parvum. students cared for animals in street clo ... | 2006 | 16672084 |
| molecular characterization of streptococcus suis strains by 16s-23s intergenic spacer polymerase chain reaction and restriction fragment length polymorphism analysis. | we developed a new molecular method of typing streptococcus suis based on polymerase chain reaction (pcr) amplification of a large fragment of rrna genes, including a part of the 16s and 23s genes and the 16s-23s intergenic spacer region (isr), followed by restriction fragment length polymorphism (rflp) analysis with rsai or mboii endonuclease. the 16s-23s isrs of 5 s. suis isolates were sequenced and compared. size and sequence polymorphisms were observed between the s735 reference strain and t ... | 2006 | 16639941 |
| antimicrobial susceptibilities of thermophilic campylobacter from humans, swine, and chicken broilers. | the aim of this study was to evaluate the incidence and the distribution of antimicrobial resistance, and the presence of genetic determinants of resistance, in campylobacter recovered from swine, poultry, and human populations in quebec. minimum inhibitory concentrations (mics) of 10 antimicrobial agents were determined by the agar dilution technique. polymerase chain reaction (pcr) was used to detect the teto determinant, and mutations in gyra were analyzed by sequencing and by mismatch amplif ... | 2006 | 16639939 |
| artificial neural network based identification of campylobacter species by fourier transform infrared spectroscopy. | two prototypes of artificial neural network (ann), multilayer perceptron (mlp), and probabilistic neural network (pnn), were used to analyze infrared (ir) spectral data obtained from intact cells belonging to the species campylobacter coli and campylobacter jejuni. in order to establish a consistent identification and typing procedure, mid infrared spectra of these species were obtained by means of a fourier transform infrared (ft-ir) spectroscope. ft-ir patterns belonging to 26 isolates subclas ... | 2006 | 16632003 |
| prevalence of thermophilic campylobacter infections in humans, chickens and crows in morogoro, tanzania. | prevalence of thermophilic campylobacter infections in humans, chickens and crows was determined in a cross-sectional study that was carried out in urban and rural areas of morogoro region, tanzania during the period of january 2003 to december 2004. a total of 632 human stool samples, 536 cloacal swabs from local and broiler chickens and 22 intestinal contents from crows were screened for presence of thermophilic campylobacters using skirrow's protocol. representative campylobacter jejuni isola ... | 2006 | 16629722 |
| amplified fragment length polymorphism, serotyping, and quinolone resistance of campylobacter jejuni and campylobacter coli strains from chicken-related samples and humans in taiwan. | the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to study the genetic relationships between campylobacter jejuni isolates from chicken-related samples (n = 32) and humans (n = 27) as well as between campylobacter coli isolates from chicken-related samples (n = 27) and humans (n = 5). these isolates were collected between 1994 and 2003 in taiwan. all c. jejuni and c. coli isolates showed highly heterogeneous fingerprints. c. jejuni isolates ... | 2006 | 16629019 |
| adherence to and invasion of human intestinal epithelial cells by campylobacter jejuni and campylobacter coli isolates from retail meat products. | the abilities of 34 campylobacter jejuni and 9 campylobacter coli isolates recovered from retail meats to adhere to and invade human intestinal epithelial t84 cells were examined and compared with those of a well-characterized human clinical strain, c. jejuni 81-176, to better assess the pathogenic potential of these meat isolates. the meat isolates exhibited a wide range of adherence and invasion abilities; a few of the isolates adhered to and invaded t84 cells almost as well as did c. jejuni 8 ... | 2006 | 16629018 |
| quantification of campylobacter on the surface and in the muscle of chicken legs at retail. | the objective of this study was to determine the prevalence and numbers of campylobacter on the skin and in the muscle of chicken legs at retail to examine the external and internal contamination for an exposure assessment. furthermore, the study assessed seasonal influence on campylobacter contamination in chicken legs. of the 140 examined skin samples, 66% were positive, and the internal contamination of 115 sampled chicken legs was 27%. the enumeration of campylobacter on the surface of posit ... | 2006 | 16629016 |
| antibiotic resistance of retail food and human campylobacter isolates on the island of ireland from 2001-2002. | the antimicrobial resistance profiles of campylobacter isolates recovered from a range of retail food samples (n=374) and humans (n=314) to eight antimicrobial compounds were investigated. high levels of resistance in food c. jejuni isolates were observed for ceftiofur (58%), ampicillin (25%) and nalidixic acid (17%) with lower levels observed for streptomycin (7.9%) and chloramphenicol (8.3%). a total of 80% of human c. jejuni isolates were resistant to ceftiofur, while 17% showed resistance to ... | 2006 | 16623987 |
| campylobacter coli enteritis and guillain-barré syndrome: no evidence of molecular mimicry and serological relationship. | campylobacter coli was isolated from two guillain-barré syndrome (gbs) patients who had anti-gm1 and anti-gd1 igg antibodies. although both this bacteria and campylobacter jejuni are common causes of diarrheal illness, previous studies have focused only on c. jejuni as the causal agent of gbs. to determine whether c. coli also is a causative agent, we examined the hypothesis that production of anti-ganglioside antibodies is induced by ganglioside-mimics on that bacterial lipo-oligosaccharide (lo ... | 2006 | 16600306 |
| a phylogenomic profile of globins. | globins occur in all three kingdoms of life: they can be classified into single-domain globins and chimeric globins. the latter comprise the flavohemoglobins with a c-terminal fad-binding domain and the gene-regulating globin coupled sensors, with variable c-terminal domains. the single-domain globins encompass sequences related to chimeric globins and "truncated" hemoglobins with a 2-over-2 instead of the canonical 3-over-3 alpha-helical fold. | 2006 | 16600051 |
| heterogeneity of campylobacter jejuni and campylobacter coli strains from healthy sheep. | the objectives of this study were to identify, at species level, thermophilic campylobacters isolated from clinically healthy sheep by a multiplex polymerase chain reaction (mpcr). the heterogeneity among campylobacter jejuni and c. coli isolates was also investigated using a restriction fragment length polymorphism (rflp) analysis of the flagellin (flaa) gene. samples of intestinal contents, gall bladders and faeces were collected from 610 healthy sheep. while gall bladder samples were plated d ... | 2006 | 16574349 |
| changes in flagellin glycosylation affect campylobacter autoagglutination and virulence. | analysis of the complete flagellin glycosylation locus of campylobacter jejuni strain 81-176 revealed a less complex genomic organization than the corresponding region in the genome strain, c. jejuni nctc 11168. twenty-four of the 45 genes found between cj1293 and cj1337 in nctc 11168 are missing in 81-176. mutation of six new genes, in addition to three previously reported, resulted in a non-motile phenotype, consistent with a role in synthesis of pseudaminic acid (pseac) or transfer of pseac t ... | 2006 | 16573682 |
| optimal settings of fingerprint-type analysing computer software for the analysis of enterohaemorrhagic escherichia coli pulsed-field gel electrophoresis patterns. | settings of fingerprint-type analysing computer software were optimized for analysis of enterohaemorrhagic escherichia coli (ehec) pulsed-field gel electrophoresis (pfge) patterns. under the lowest values of parameters, maximum value of similarities calculated using the dice coefficient were obtained between pfge patterns from one ehec strain on the same gel when reference lanes for calibration of distortions during electrophoresis were set to every fourth lane. pfge patterns of 15 ehec strains ... | 2006 | 16566856 |
| two distinct mutations in gyra lead to ciprofloxacin and nalidixic acid resistance in campylobacter coli and campylobacter jejuni isolated from chickens and beef cattle. | the aim of this study was to identify point mutations in the gyra quinolone resistance determining region (qrdr) of campylobacter coli (n = 27) and campylobacter jejuni (n = 26) that confer nalidixic acid (nal) resistance without conferring resistance to ciprofloxacin (cip). | 2006 | 16553723 |
| enumeration and identity of campylobacter spp. in italian broilers. | transmission of campylobacter to humans has been prominently associated with mishandling or improperly preparing contaminated poultry carcasses. the number of organisms per carcass represents an important measure of human exposure to the agent. therefore, we wished to estimate this public exposure over 1 yr among italian broiler carcasses. we sampled 213 broiler carcasses from rinse water samples collected from a single slaughterhouse. groups of carcasses had mean processed weights ranging from ... | 2006 | 16553289 |
| the helicobacter pylori chemotaxis receptor tlpb (hp0103) is required for ph taxis and for colonization of the gastric mucosa. | the location of helicobacter pylori in the gastric mucosa of mammals is defined by natural ph gradients within the gastric mucus, which are more alkaline proximal to the mucosal epithelial cells and more acidic toward the lumen. we have used a microscope slide-based ph gradient assay and video data collection system to document ph-tactic behavior. in response to hydrochloric acid (hcl), h. pylori changes its swimming pattern from straight-line random swimming to arcing or circular patterns that ... | 2006 | 16547053 |
| genome-wide screen for salmonella genes required for long-term systemic infection of the mouse. | a microarray-based negative selection screen was performed to identify salmonella enterica serovar typhimurium (serovar typhimurium) genes that contribute to long-term systemic infection in 129x1/svj (nramp1(r)) mice. a high-complexity transposon-mutagenized library was used to infect mice intraperitoneally, and the selective disappearance of mutants was monitored after 7, 14, 21, and 28 d postinfection. one hundred and eighteen genes were identified to contribute to serovar typhimurium infectio ... | 2006 | 16518469 |
| comparison of culture and a novel 5' taq nuclease assay for direct detection of campylobacter fetus subsp. venerealis in clinical specimens from cattle. | a campylobacter fetus subsp. venerealis-specific 5' taq nuclease pcr assay using a 3' minor groove binder-dna probe (taqman mgb) was developed based on a subspecies-specific fragment of unknown identity (s. hum, k. quinn, j. brunner, and s. l. on, aust. vet. j. 75:827-831, 1997). the assay specifically detected four c. fetus subsp. venerealis strains with no observed cross-reaction with c. fetus subsp. fetus-related campylobacter species or other bovine venereal microflora. the 5' taq nuclease a ... | 2006 | 16517880 |
| activities of telithromycin, erythromycin, fluoroquinolones, and doxycycline against campylobacter strains isolated from finnish subjects. | the in vitro susceptibilities of 478 campylobacter jejuni and campylobacter coli strains isolated from finnish subjects during 2002 to 2004 were determined. susceptibility to erythromycin remained high, and telithromycin did not offer any advantage over erythromycin. reduced susceptibilities to fluoroquinolones and doxycycline were detected almost exclusively among isolates of foreign origin. | 2006 | 16495275 |
| measurement of the viability of lawsonia intracellularis. | the objective of this study was to develop and test both a flow cytometry method (fcm) and a direct count method (dcm) that both use fluorescent stains to determine the viability of lawsonia intracellularis (li), an obligate intracellular bacterium and the cause of proliferative enteropathy (pe) in pigs and other animal species. live li were passaged in cell culture and harvested from infected mccoy cells. dead li were prepared by exposing live li to 70% isopropyl alcohol for 30 min. seven sampl ... | 2005 | 16479724 |
| random amplified polymorphic dna analysis of campylobacter jejuni and campylobacter coli isolated from healthy cattle and sheep. | the genetic heterogeneity among campylobacter jejuni and campylobacter coli isolates obtained from apparently healthy cattle and sheep was investigated by random amplified polymorphic dna (rapd) analysis. a total of 348 campylobacter isolates, consisting of c. jejuni (n = 218) and c. coli (n = 130), were analysed. all these isolates were successfully typed by rapd analysis. the total numbers of band patterns defined by rapd in cattle and sheep were 42 and 45, respectively. of the 42 distinct typ ... | 2006 | 16476798 |
| natural transformation-mediated transfer of erythromycin resistance in campylobacter coli strains from turkeys and swine. | erythromycin resistance in campylobacter coli from meat animals is frequently encountered and could represent a substantial barrier to antibiotic treatment of human infections. erythromycin resistance in this organism has been associated with a point mutation (a2075g) in the 23s rrna gene. however, the mechanisms responsible for possible dissemination of erythromycin resistance in c. coli remain poorly understood. in this study, we investigated transformation-mediated acquisition of erythromycin ... | 2006 | 16461682 |
| isolation and characterization of a cyanophage infecting the toxic cyanobacterium microcystis aeruginosa. | we isolated a cyanophage (ma-lmm01) that specifically infects a toxic strain of the bloom-forming cyanobacterium microcystis aeruginosa. transmission electron microscopy showed that the virion is composed of anisometric head and a tail complex consisting of a central tube and a contractile sheath with helical symmetry. the morphological features and the host specificity suggest that ma-lmm01 is a member of the cyanomyovirus group. using semi-one-step growth experiments, the latent period and bur ... | 2006 | 16461672 |
| application of pulsed-field gel electrophoresis to identify potential outbreaks of campylobacteriosis in new zealand. | since 2002, new zealand's incidence of campylobacteriosis has exceeded 300 cases per 100,000 people per annum. to evaluate genetic variation in human isolates, 183 campylobacter isolates were collected from a single clinical laboratory in christchurch: 77 during an 8-week period in spring, and the rest 3 months later over a second 8-week period in autumn. isolates were identified to the species level and subtyped using penner serotyping (campylobacter jejuni only) and pulsed-field gel electropho ... | 2006 | 16455892 |
| distribution of tetracycline resistance genes in actinobacillus pleuropneumoniae isolates from spain. | actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia. tetracycline is used for therapy of this disease, and a. pleuropneumoniae carrying the tet(b) gene, coding for an efflux protein that reduces the intercellular tetracycline level, has been described previously. of the 46 tetracycline-resistant (tc(r)) spanish a. pleuropneumoniae isolates used in this study, 32 (70%) carried the tet(b) gene, and 30 of these genes were associated with plasmids. eight (17%) isolate ... | 2006 | 16436729 |
| australian multicentre comparison of subtyping methods for the investigation of campylobacter infection. | in order to identify subtyping methods able to contribute to the surveillance or investigation of australian campylobacter infection, six genotypic and three phenotypic subtyping methods were evaluated on a collection of 84 clinical isolates collected over a 30-month period from one region in australia. the aim was to compare the logistics of various subtyping methods and examine their ability to assist in finding outbreaks or common sources of sporadic infection. the genotypic subtyping methods ... | 2006 | 16436218 |
| epidemiology of campylobacter jejuni and campylobacter coli infections in the zenica--doboj canton, bosnia and herzegovina--a laboratory based surveillance in the 1999-2001 period. | previous studies in the zenica--doboj canton, bosnia and herzegovina, indicated some different epidemiological features of campylobacter infections and high degree of antimicrobial resistance. therefore, it was important to investigate epidemiology of campylobacter jejuni and campylobacter coli infections by demographic features and antimicrobial resistance in the 1999-2001 period. a total number of 40 (75.5%) c. jejuni and 13 (24.5%) c. coli non-repeated clinical isolates were analyzed. more th ... | 2005 | 16417178 |
| campylobacter contamination during poultry slaughter in belgium. | the relation between internal carriage and surface contamination with thermophilic campylobacter species in broilers was examined by molecular typing methods. samples from 39 flocks were collected in three belgian poultry slaughterhouses. from each flock, crop swabs before slaughter and intestines and neck skins during slaughter were collected. a total of 309 isolates were identified at species level and further characterized by flagellin gene a pcr/restriction fragment length polymorphism and p ... | 2006 | 16416897 |