Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| phenytoin-induced gingival overgrowth: a review of the molecular, immune, and inflammatory features. | gingival overgrowth (go) is a side effect associated with some distinct classes of drugs, such as anticonvulsants, immunosuppressant, and calcium channel blockers. go is characterized by the accumulation of extracellular matrix in gingival connective tissues, particularly collagenous components, with varying degrees of inflammation. one of the main drugs associated with go is the antiepileptic phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. nevertheless, th ... | 2011 | 21991476 |
| composition and localization of treponema denticola outer membrane complexes. | the treponema denticola outer membrane lipoprotein-protease complex (dentilisin) contributes to periodontal disease by degrading extracellular matrix components and disrupting intercellular host signaling pathways. we recently demonstrated that prcb, located upstream of and cotranscribed with prca and prtp, encodes a 22-kda lipoprotein that interacts with prtp and is required for its activity. here we further characterize products of the protease locus and their roles in expression, formation, a ... | 2011 | 21986628 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| critical analysis of biomarkers in the current periodontal practice. | periodontal disease is a chronic microbial infection that triggers inflammation-mediated loss of the periodontal ligament and alveolar bone that supports the teeth. because of the increasing prevalence and associated comorbidities, there is a need for the development of new diagnostic tests that can detect the presence of active disease, predict future disease progression, and evaluate the response to periodontal therapy, thereby improving the clinical management of periodontal patients. the dia ... | 2011 | 21976831 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| life cycle stage-resolved proteomic analysis of the excretome/secretome from strongyloides ratti--identification of stage-specific proteases. | a wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. excretory and secretory proteins (esp) are active at the interface between parasite and host and comprise potential targets for intervention. the intestinal nematode strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living gener ... | 2011 | 21964353 |
| pathogenic microbes and community service through manipulation of innate immunity. | the periodontal pathogen porphyromonas gingivalis undermines major components of innate immunity, such as complement, toll-like receptors (tlr), and their crosstalk pathways. at least in principle, these subversive activities could promote the adaptive fitness of the entire periodontal biofilm community. in this regard, the virulence factors responsible for complement and tlr exploitation (gingipain enzymes, atypical lipopolysaccharide molecules, and fimbriae) are released as components of readi ... | 2012 | 21948363 |
| salivary levels of antibacterial peptide (ll-37/hcap-18) and cotinine in periodontitis patients. | background: the purpose of this study was to examine the relationship between salivary ll-37 levels and clinical severity in chronic periodontitis (cp) patients. the presence/absence of four periodontopathic bacteria and salivary cotinine levels were also examined in order to assess the impact of these factors on ll-37 production. methods: unstimulated salivary samples were collected from 69 cp patients. salivary concentrations of ll-37 and cotinine were measured by enzyme-linked immunosorbent ... | 2011 | 21942788 |
| colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. | gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ... | 2011 | 21929616 |
| detection of periodontal pathogens in newborns and children with mixed dentition. | we report the age-related prevalence of red complex periodontal pathogens, porphyromonas gingivalis, treponema denticola, and tannerella forsythia, along with four strains of orange complex pathogens. the bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (pcr). p. gingivalis was not detected in any site of any subject in the two groups tested. however, t. denticola w ... | 2011 | 21928086 |
| deciphering morphological determinants of the helix-shaped leptospira. | leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiology of these pathogens. in this study, we showed that peptidoglycan sacculi from leptospira spp. retain the helical shape of intact cells. interestingly, the distribution of muropept ... | 2011 | 21926230 |
| s100a2 level changes are related to human periodontitis. | periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life. s100a2 was previously identified as a component of gingival crevicular fluid (gcf) via proteome analysis, but it has not been investigated whether s100a2 plays a role in periodontitis. in this study, we analyzed mrna expression of s100a2 in gingival tissues from normal and classified periodontal disease patients and compared it to that of s100a8 and s100a9. quantitative real ... | 2011 | 21922197 |
| translational and clinical applications of salivary diagnostics. | there have been significant advances in techniques for the detection of biomarker signals in the oral cavity (e.g., elisas for proteins, pcr for rna and dna) as well as the engineering and development of microfluidic approaches to make oral-based point-of-care (poc) methods for the diagnosis for both local and systemic conditions a reality. in this section, we focus on three such approaches, namely, periodontal disease management, early markers for systemic diseases, and salivary markers useful ... | 2011 | 21917748 |
| molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan. | periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ... | 2011 | 21916371 |
| Co-expression of Arabidopsis thaliana phytochelatin synthase and Treponema denticola cysteine desulfhydrase for enhanced arsenic accumulation. | Arsenic is one of the most hazardous pollutants found in aqueous environments and has been shown to be a carcinogen. Phytochelatins (PCs), which are cysteine-rich and thio-reactive peptides, have high binding affinities for various metals including arsenic. Previously, we demonstrated that genetically engineered Saccharomyces cerevisiae strains expressing phytochelatin synthase (AtPCS) produced PCs and accumulated arsenic. In an effort to further improve the overall accumulation of arsenic, cyst ... | 2012 | 21915851 |
| characterization and scope of s-layer protein o-glycosylation in tannerella forsythia. | cell surface glycosylation is an important element in defining the life of pathogenic bacteria. tannerella forsythia is a gram-negative, anaerobic periodontal pathogen inhabiting the subgingival plaque biofilms. it is completely covered by a two-dimensional crystalline surface layer (s-layer) composed of two glycoproteins. although the s-layer has previously been shown to delay the bacterium's recognition by the innate immune system, we characterize here the s-layer protein o-glycosylation as a ... | 2011 | 21911490 |
| the capsule of porphyromonas gingivalis leads to a reduction in the host inflammatory response, evasion of phagocytosis, and increase in virulence. | periodontal disease is a chronic oral inflammatory disease that is triggered by bacteria such as porphyromonas gingivalis. p. gingivalis strains exhibit great heterogeneity, with some strains being encapsulated while others are nonencapsulated. although the encapsulated strains have been shown to be more virulent in a mouse abscess model, so far the role of the capsule in p. gingivalis interactions with host cells is not well understood and its role in virulence has not been defined. here, we in ... | 2011 | 21911459 |
| microbiologic findings 1 year after partial- and full-mouth scaling in the treatment of moderate chronic periodontitis. | to investigate the impact on microbiologic variables of full-mouth scaling (fms) and conventional scaling and root planing (csrp) after 12 months. | 2011 | 21909493 |
| Treponema denticola major outer sheath protein induces actin assembly at free barbed ends by a PIP2-dependent uncapping mechanism in fibroblasts. | The major outer sheath protein (Msp) of Treponema denticola perturbs actin dynamics in fibroblasts by inducing actin reorganization, including subcortical actin filament assembly, leading to defective calcium flux, diminished integrin engagement of collagen, and retarded cell migration. Yet, its mechanisms of action are unknown. We challenged Rat-2 fibroblasts with enriched native Msp. Msp activated the small GTPases Rac1, RhoA and Ras, but not Cdc42, yet only Rac1 localized to areas of actin re ... | 2011 | 21901132 |
| polymicrobial periodontal pathogen transcriptomes in calvarial bone and soft tissue. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia are consistently associated with adult periodontitis. this study sought to document the host transcriptome to a p. gingivalis, t. denticola, and t.forsythia challenge as a polymicrobial infection using a murine calvarial model of acute inflammation and bone resorption. mice were infected with p. gingivalis, t. denticola, and t. forsythia over the calvaria, after which the soft tissues and calvarial bones were excised. a muri ... | 2011 | 21896157 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| role of sialidase in glycoprotein utilization by tannerella forsythia. | the major bacterial pathogens associated with periodontitis include tannerella forsythia. we previously discovered that sialic acid stimulates biofilm growth of t. forsythia, and that sialidase activity is key to utilization of sialoconjugate sugars and is involved in host-pathogen interactions in vitro. the aim of this work was to assess the influence of the nanh sialidase on initial biofilm adhesion and growth in experiments where the only source of sialic acid was sialoglycoproteins or human ... | 2011 | 21885482 |
| genetic organisation, mobility and predicted functions of genes on integrated, mobile genetic elements in sequenced strains of clostridium difficile. | clostridium difficile is the leading cause of hospital-associated diarrhoea in the us and europe. recently the incidence of c. difficile-associated disease has risen dramatically and concomitantly with the emergence of 'hypervirulent' strains associated with more severe disease and increased mortality. c. difficile contains numerous mobile genetic elements, resulting in the potential for a highly plastic genome. in the first sequenced strain, 630, there is one proven conjugative transposon (ctn) ... | 2011 | 21876735 |
| Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelement. | Anticipatory ligand binding through massive protein sequence variation is rare in biological systems, having been observed only in the vertebrate adaptive immune response and in a phage diversity-generating retroelement (DGR). Earlier work has demonstrated that the prototypical DGR variable protein, major tropism determinant (Mtd), meets the demands of anticipatory ligand binding by novel means through the C-type lectin (CLec) fold. However, because of the low sequence identity among DGR variabl ... | 2011 | 21873231 |
| the rela/spot homolog (rsh) superfamily: distribution and functional evolution of ppgpp synthetases and hydrolases across the tree of life. | rela/spot homologue (rsh) proteins, named for their sequence similarity to the rela and spot enzymes of escherichia coli, comprise a superfamily of enzymes that synthesize and/or hydrolyze the alarmone ppgpp, activator of the "stringent" response and regulator of cellular metabolism. the classical "long" rshs rel, rela and spot with the ppgpp hydrolase, synthetase, tgs and act domain architecture have been found across diverse bacteria and plant chloroplasts, while dedicated single domain ppgpp- ... | 2011 | 21858139 |
| Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. | Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ... | 2011 | 21853142 |
| epithelial interleukin-8 responses to oral bacterial biofilms. | an in vitro model of bacterial biofilms on rigid gas-permeable contact lenses (rgpls) was developed to challenge oral epithelial cells. this novel model provided seminal data on oral biofilm-host cell interactions, and with selected bacteria, the biofilms were more effective than their planktonic counterparts at stimulating host cell responses. | 2011 | 21832101 |
| Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy. | BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ... | 2011 | 21830010 |
| benzyl isothiocyanate, a major component from the roots of salvadora persica is highly active against gram-negative bacteria. | plants produce a number of antimicrobial substances and the roots of the shrub salvadora persica have been demonstrated to possess antimicrobial activity. sticks from the roots of s. persica, miswak sticks, have been used for centuries as a traditional method of cleaning teeth. diverging reports on the chemical nature and antimicrobial repertoire of the chewing sticks from s. persica led us to explore its antibacterial properties against a panel of pathogenic or commensal bacteria and to identif ... | 2011 | 21829688 |
| microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. | abstract objective: to evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (pcr) method. materials and methods: thirty orthodontic patients (17 females and 13 males; aged 16.7 -¦ 6.5-áy) were included in this study. subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left ... | 2011 | 21827233 |
| filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis. | filifactor alocis, a gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. there is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. to evaluate the virulence potential of f. alocis and its ability to interact with porphyromonas gingivalis w83, several clinical isolates of f. alocis were characterized. f. alocis showed nongingipain protease and sialidase activitie ... | 2011 | 21825062 |
| measurement of peroxiredoxin activity. | peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. protocols are provided to measure prx activity with peroxide by (1) a coupled reaction with nadph, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the fox assay. | 2011 | 21818753 |
| alzheimer's disease - a neurospirochetosis. analysis of the evidence following koch's and hill's criteria. | abstract: it is established that chronic spirochetal infection can cause slowly progressive dementia, brain atrophy and amyloid deposition in late neurosyphilis. recently it has been suggested that various types of spirochetes, in an analogous way to treponema pallidum, could cause dementia and may be involved in the pathogenesis of alzheimer's disease (ad). here, we review all data available in the literature on the detection of spirochetes in ad and critically analyze the association and causa ... | 2011 | 21816039 |
| diversity and plasticity of the intracellular plant pathogen and insect symbiont, 'candidatus liberibacter asiaticus', revealed by hyper variable prophage genes with intragenic tandem repeats. | 'candidatus liberibacter asiaticus' (las) is a psyllid-transmitted, phloem-limited alpha proteobacterium, and the most prevalent species of liberibacter associated with a devastating worldwide citrus disease known as huanglongbing (hlb). two related and hyper variable genes (hyvi and hyvii) were identified in the prophage regions of the psy62 las genome. sequence analyses of the hyvi/hyvii genes in 35 las dna isolates collected globally revealed hyvi gene contains up to12 nearly identical tandem ... | 2011 | 21784907 |
| bacterial interactions in dental biofilm. | biofilms are masses of microorganisms that bind to and multiply on a solid surface, typically with a fluid bathing the microbes. the microorganisms that are not attached but are free floating in an aqueous environment are termed planktonic cells. traditionally, microbiology research has addressed results from planktonic bacterial cells. however, many recent studies have indicated that biofilms are the preferred form of growth of most microbes and particularly those of a pathogenic nature. biofil ... | 2011 | 21778817 |
| pulpal perio relations: interdisciplinary diagnostic approach - i. | lesions of pulpal and periodontal origin may perpetuate from either the infections of dental pulp or periodontium or alveolar bone. this review focuses on interdisciplinary diagnostic approach towards lesions of periodontal or endodontic origin. | 2011 | 21772729 |
| evaluation of local drug-delivery system containing 2% whole turmeric gel used as an adjunct to scaling and root planing in chronic periodontitis: a clinical and microbiological study. | to compare the effect of experimental local-drug delivery system containing 2% whole turmeric (gel form) as an adjunct to scaling and root planing (srp) with the effect achieved using srp alone by assessing their respective effects on plaque, gingival inflammation, bleeding on probing pocket depth, relative attachment levels and trypsin-like enzyme activity of "red complex" microorganisms, namely, bacteroides forsythus, porphvromonas gingivalis and treponema denticola. | 2011 | 21772719 |
| probiotics in periodontal health and disease. | macfarlane burnett stated in 1962 that "by the late twentieth century, we can anticipate the virtual elimination of infectious diseases as a significant factor in social life". probiotics have become of interest to researchers in recent times. time has come to shift the paradigm of treatment from specific bacteria elimination to altering bacterial ecology by probiotics. the development of resistance to a range of antibiotics by some important pathogens has raised the possibility of a return to t ... | 2011 | 21772717 |
| detection of periodontal markers in chronic periodontitis. | the aim was to compare the detection frequency of periodontopathogens by using the pado test 4.5 and checkerboard dna-dna hybridization technique in chronic periodontitis patients.thirty patients with chronic periodontitis were tested cross-sectionally with dna/rna oligogenomic probe method (iai pado test 4.5) and dna/dna whole genomic probe (checkerboard) method. samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the t ... | 2011 | 21769304 |
| characterization of novel ompa-like protein of leptospira interrogans that binds extracellular matrix molecules and plasminogen. | leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease of human and veterinary concern. the identification of novel proteins that mediate host-pathogen interactions is important for understanding the bacterial pathogenesis as well as to identify protective antigens that would help fight the disease. we describe in this work the cloning, expression, purification and characterization of three predicted leptospiral membrane proteins, lic10258, lic12880 (lp30) and lic12 ... | 2011 | 21755014 |
| getting the measure of syphilis: qpcr to better understand early infection. | objectives until recently, pcr had been used to detect but not quantify treponema pallidum. to understand infection kinetics of this uncultivable organism, a real-time pcr assay was developed to quantify 47 kda membrane lipoprotein gene dna (tpp47). methods assay specificity was determined against dna from humans, skin organisms and sexually transmitted pathogens. tpp47 dna (nichols strain) was used to construct a standard curve for t pallidum quantification. blood and ulcer samples were ... | 2011 | 21752804 |
| caloric restriction and chronic inflammatory diseases. | oral diseases (2011) a reduction in calorie intake [caloric restriction (cr)] appears to consistently decrease the biological rate of aging in a variety of organisms as well as protect against age-associated diseases including chronic inflammatory disorders such as cardiovascular disease and diabetes. although the mechanisms behind this observation are not fully understood, identification of the main metabolic pathways affected by cr has generated interest in finding molecular targets that could ... | 2011 | 21749581 |
| caloric restriction and chronic inflammatory diseases. | oral diseases (2011) a reduction in calorie intake [caloric restriction (cr)] appears to consistently decrease the biological rate of aging in a variety of organisms as well as protect against age-associated diseases including chronic inflammatory disorders such as cardiovascular disease and diabetes. although the mechanisms behind this observation are not fully understood, identification of the main metabolic pathways affected by cr has generated interest in finding molecular targets that could ... | 2011 | 21749581 |
| clinical and microbiological results 12 months after scaling and root planing with different irrigation solutions in patients with moderate chronic periodontitis ?a pilot randomized trial. | aim: the aim of this study was to determine in a randomized trial the impact on treatment outcome after 12 months of different subgingival irrigation solutions during scaling and root planing (srp). materials and methods: 51 adult volunteers with generalized chronic periodontitis were treated by full mouth srp using 0.9 % sodium chloride, 0.12 % chlorhexidine digluconate or 7.5 % povidone-iodine for subgingival irrigation during srp. prior to srp, after three and twelve months probing depth (pd) ... | 2011 | 21749169 |
| analysis of the peri-implant microbiota in 90 dental implants and its relationship to crevicular fluid volume. | objective: to evaluate the presence within the peri-implant sulcus of tannerela forsythia (tf), porphyromonas gingivales (pg), treponema denticola (td) and aggregatibacter actinomycetemcomitans (aa), and relate these bacteria to the peri-implant crevicular fluid volume (picfv). material and method: a prospective and cross-sectional clinical case series study was made. for the measurement of crevicular fluid, use was made of the periotron® 8000 (proflow incorporated. new york, usa), measuring the ... | 2011 | 21743411 |
| diabetes and periodontitis. | the main aim of this review is to update the reader with practical knowledge concerning the relationship between diabetes mellitus and periodontal diseases. exclusive data is available on the association between these two chronic diseases till date. articles published on this relationship often provide the knowledge of definitions of diabetes mellitus and periodontal diseases, prevalence, extent, severity of periodontal disease, complications of diabetes along with the possible underlying mechan ... | 2010 | 21731243 |
| synergistic virulence of porphyromonas gingivalis and treponema denticola in a murine periodontitis model. | chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing porphyromonas gingivalis and treponema denticola. we have investigated the potential synergistic virulence of p. gingivalis and t. denticola using a murine experimental model of periodontitis. an inoculation regime of four intra-oral doses of 1 +ù 10(10) p. gingivalis cells induced significant periodontal bone lo ... | 2011 | 21729244 |
| clinical, microbiologic, and immunologic factors of orthodontic treatment-induced gingival enlargement. | the aim of this study was to investigate the microbiologic and immunologic factors related to orthodontic treatment-induced gingival enlargement (ge). | 2011 | 21724088 |
| Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA). | The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated. | 2011 | 21711368 |
| comparative genome analysis and genome-guided physiological analysis of roseobacter litoralis. | roseobacter litoralis och149, the type species of the genus, and roseobacter denitrificans och114 were the first described organisms of the roseobacter clade, an ecologically important group of marine bacteria. both species were isolated from seaweed and are able to perform aerobic anoxygenic photosynthesis. | 2011 | 21693016 |
| [prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis]. | helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori. | 2011 | 21688602 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| complete genome sequence of treponema paraluiscuniculi, strain cuniculi a: the loss of infectivity to humans is associated with genome decay. | treponema paraluiscuniculi is the causative agent of rabbit venereal spirochetosis. it is not infectious to humans, although its genome structure is very closely related to other pathogenic treponema species including treponema pallidum subspecies pallidum, the etiological agent of syphilis. in this study, the genome sequence of treponema paraluiscuniculi, strain cuniculi a, was determined by a combination of several high-throughput sequencing strategies. whereas the overall size (1,133,390 bp), ... | 2011 | 21655244 |
| crystallization of the factor h-binding protein, fhbb, from the periopathogen treponema denticola. | treponema denticola is a primary etiological agent of periodontal disease. t. denticola evades complement-mediated killing by binding to the host's factor h (fh), a negative regulator of the alternative complement pathway. the t. denticola fh-binding protein has been identified and designated as factor h-binding protein b (fhbb). crystals of recombinant fhbb were obtained by the hanging-drop vapor-diffusion method using sodium citrate and 0.2 m sodium thiocyanate. fhbb crystals diffracted to 1.8 ... | 2011 | 21636910 |
| the pathogenic persona of community-associated oral streptococci. | the mitis group streptococci (mgs) are widespread in the oral cavity and are traditionally associated with oral health. however, these organisms have many attributes that contribute to the development of pathogenic oral communities. mgs adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment substratum for more overtly pathogenic organisms such as porphyromonas gingivalis, and the two species assemble into heterotypic communities. close physical association facilitates ph ... | 2011 | 21635580 |
| tlr2 signaling and th2 responses drive tannerella forsythia-induced periodontal bone loss. | periodontal disease (pd) is a chronic inflammation of the tooth-supporting soft tissue and alveolar bone due to infection by a select group of gram-negative microbes, which leads to tooth loss if untreated. because mice deficient in cd4(+) cells are resistant to infection-induced alveolar bone loss, th cells have been implicated in bone-destructive processes during pd. however, the extent to which different th cell subtypes play roles in pathogenesis or host protection remains to be defined and ... | 2011 | 21632710 |
| experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing. | the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed. | 2011 | 21625744 |
| riboswitch regulates a thiamine pyrophosphate (tpp) abc transporter of the oral spirochete treponema denticola. | thiamine pyrophosphate (tpp), a biologically active form of thiamine (vitamin b1), is an essential cofactor in all living systems. microorganisms either synthesize tpp via de novo biosynthesis pathways or uptake exogenous thiamine from the environment via specific transporters. the oral spirochete treponema denticola is an important pathogen that is associated with human periodontal diseases. it lacks a de novo tpp biosynthesis pathway and needs exogenous tpp for the growth, suggesting that it m ... | 2011 | 21622748 |
| identification of periodontal pathogens in healthy periimplant sites. | : the aim of this study was to evaluate the presence of periodontopathogens in subgingival periimplant sites in partially edentulous patients using polymerase chain reaction procedures, with regard to areas with clinical and radiographic signs of health and areas presenting periimplant disease. | 2011 | 21613949 |
| levels of serum igg specific to the bspa protein of tannerella forsythia are related to the periodontal status. | background: tannerella forsythia is a gram-negative anaerobe implicated in the development of periodontal disease (pd). bspa is a surface expressed and secreted protein, recognized as an important virulence factor of t. forsythia. this study was undertaken to determine if t. forsythia bacterial surface protein a (bspa) induces an antibody response in pd. we hypothesized that serum immunoglobulin g (igg) antibody levels against bspa will correlate with patient's disease. methods: sera were obtain ... | 2011 | 21609257 |
| levels of serum igg specific to the bspa protein of tannerella forsythia are related to the periodontal status. | background: tannerella forsythia is a gram-negative anaerobe implicated in the development of periodontal disease (pd). bspa is a surface expressed and secreted protein, recognized as an important virulence factor of t. forsythia. this study was undertaken to determine if t. forsythia bacterial surface protein a (bspa) induces an antibody response in pd. we hypothesized that serum immunoglobulin g (igg) antibody levels against bspa will correlate with patient's disease. methods: sera were obtain ... | 2011 | 21609257 |
| disruption of a type ii endonuclease (tde0911) enables treponema denticola atcc 35405 strain to accept an unmethylated shuttle vector. | the oral spirochete treponema denticola is associated with human periodontal disease. t. denticola atcc 35405 (td(35405)) and 33520 (td(33520)) are two routinely used laboratory strains. compared to td(33520), td(35405) is more virulent but less accessible to genetic manipulations. for instance, the shuttle vectors of td(33520) cannot be transformed into td(35405). the lack of a shuttle vector has been a barrier to study the biology and virulence of td(35405). in this report, we hypothesize that ... | 2011 | 21602384 |
| metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. | production and chemicals or fuels directly from co(2) is an attractive approach to solving the energy and environmental problems. 1-butanol, a chemical feedstock and potential fuel, has been produced by fermentation of carbohydrates, both in native clostridium species and various engineered hosts. to produce 1-butanol from co(2), we transferred a modified coa-dependent 1-butanol production pathway into a cyanobacterium, synechococcus elongatus pcc 7942. we demonstrated the activity of each enzym ... | 2011 | 21569861 |
| diagnostic evaluation of a nanobody with picomolar affinity toward the protease rgpb from porphyromonas gingivalis. | porphyromonas gingivalis is one of the major periodontitis-causing pathogens. p. gingivalis secretes a group of proteases termed gingipains, and in this study we have used the rgpb gingipain as a biomarker for p. gingivalis. we constructed a naive camel nanobody library and used phage display to select one nanobody toward rgpb with picomolar affinity. the nanobody was used in an inhibition assay for detection of rgpb in buffer as well as in saliva. the nanobody was highly specific for rgpb given ... | 2011 | 21569755 |
| identification of mono- or poly-specific monoclonal antibody to porphyromonas gingivalis heat-shock protein 60. | the aim of this study was to define the immunoreactive specificity of porphyromonas gingivalis (p. gingivalis) heat shock protein (hsp) 60 in periodontitis and atherosclerosis. | 2011 | 21556254 |
| treponema denticola pure is a bacterial air carboxylase. | de novo purine biosynthesis proceeds by two divergent paths. in bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (air) is converted to 4-carboxy-air (cair) by two enzymes: n(5)-carboxy-air (n(5)-cair) synthetase (purk) and n(5)-cair mutase (class i pure). in animals, the conversion of air to cair requires a single enzyme, air carboxylase (class ii pure). the cair carboxylate derives from bicarbonate or co(2), respectively. class i pure is a promising antimicrobial target. class i an ... | 2011 | 21548610 |
| streptococcus mutans strains recovered from caries-active or caries-free individuals differ in sensitivity to host antimicrobial peptides. | antimicrobial peptides (amps) are among the repertoire of host innate immune defenses. in the oral cavity, several amps are present in saliva and have antimicrobial activities against oral bacteria, including streptococcus mutans, a primary etiological agent of dental caries. in this study, we hypothesized that unique s. mutans strains, as determined by dna fingerprinting from sixty 13-year-old subjects with or without experience of caries, would have different susceptibilities to α-defensins-1- ... | 2011 | 21545696 |
| effect of periodontopathogen lipopolysaccharides and proinflammatory cytokines on cd46, cd55, and cd59 gene/protein expression by oral epithelial cells. | membrane-anchored complement regulatory proteins (crps), including cd46, cd55, and cd59, protect host cells from complement attack. in the present study, we investigated whether periodontopathogen lipopolysaccharide and proinflammatory cytokines modulate crp gene/protein expression in human oral epithelial cells. the lipopolysaccharide of treponema denticola and tannerella forsythia were the most potent for increasing the gene expression of cd55 and cd59, and to a lesser extent cd46, after a 48- ... | 2011 | 21545652 |
| the diguanylate cyclase, rrp1, regulates critical steps in the enzootic cycle of the lyme disease spirochetes. | rrp1 is the sole c-di-gmp-producing protein (diguanylate cyclase) of borrelia burgdorferi. to test the hypothesis that rrp1 regulates critical processes involved in the transmission of spirochetes between ticks and mammals, an rrp1 deletion mutant (b31-δrrp1) and a strain that constitutively produces elevated levels of rrp1 (b31-ov) were constructed. the strains were assessed for progression through the enzootic cycle using an ixodes tick/c3h-hej mouse model and tick immersion feeding methods. b ... | 2011 | 21542866 |
| dendritic cells, antibodies reactive with oxldl, and inflammation. | periodontitis appears to promote chronic inflammatory diseases, including atherosclerosis, but relevant mechanisms need clarification. oral bacteria induce antibodies that bind not only bacteria, but also oxldl. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans induce remarkable igg responses that are dominated by igg2, and igg2 is ifn-γ-dependent and is promoted by dendritic cells (dcs). ldl-reactive antibodies induced by p. gingivalis and a. actinomycetemcomitans include anti- ... | 2011 | 21531918 |
| chey3 of borrelia burgdorferi is the key response regulator essential for chemotaxis and forms a long-lived phosphorylated intermediate. | spirochetes have a unique cell structure: these bacteria have internal periplasmic flagella subterminally attached at each cell end. how spirochetes coordinate the rotation of the periplasmic flagella for chemotaxis is poorly understood. in other bacteria, modulating flagellar rotation is essential for chemotaxis, and phosphorylation-dephosphorylation of the response regulator chey plays a key role in regulating this rotary motion. the genome of the lyme disease spirochete borrelia burgdorferi c ... | 2011 | 21531807 |
| prevalence of sos-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons. | abstract: | 2011 | 21529368 |
| differential effects of periopathogens on host protease inhibitors slpi, elafin, scca1, and scca2. | secretory leukocyte peptidase inhibitors (slpi), elafin, squamous cell carcinoma antigen 1 and 2 (scca1 and scca2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. to determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (gecs) challenged with cell-free bacteria supernatants of various periopathogens porphyr ... | 2010 | 21523231 |
| oral microbiota and cancer. | inflammation caused by infections may be the most important preventable cause of cancer in general. however, in the oral cavity the role of microbiota in carcinogenesis is not known. microbial populations on mouth mucosa differ between healthy and malignant sites and certain oral bacterial species have been linked with malignancies but the evidence is still weak in this respect. nevertheless, oral microorganisms inevitably up-regulate cytokines and other inflammatory mediators that affect the co ... | 2010 | 21523227 |
| preterm low birthweight and the role of oral bacteria. | preterm and low birthweight (ptlbw) continues to be a major cause of mortality and morbidity across the world. in recent years, maternal periodontal disease has been implicated as a risk factor for adverse pregnancy outcomes. there is conflicting evidence to support such an outcome as illustrated by descriptive, case control and randomised controlled trials involving pregnant women from across the world, using different measurement tools to determine the level of periodontal disease. whilst cons ... | 2010 | 21523222 |
| cardiovascular disease and the role of oral bacteria. | in terms of the pathogenesis of cardiovascular disease (cvd) the focus has traditionally been on dyslipidemia. over the decades our understanding of the pathogenesis of cvd has increased, and infections, including those caused by oral bacteria, are more likely involved in cvd progression than previously thought. while many studies have now shown an association between periodontal disease and cvd, the mechanisms underpinning this relationship remain unclear. this review gives a brief overview of ... | 2010 | 21523220 |
| porphyromonas gingivalis-dendritic cell interactions: consequences for coronary artery disease. | an estimated 80 million us adults have one or more types of cardiovascular diseases. atherosclerosis is the single most important contributor to cardiovascular diseases; however, only 50% of atherosclerosis patients have currently identified risk factors. chronic periodontitis, a common inflammatory disease, is linked to an increased cardiovascular risk. dendritic cells (dcs) are potent antigen presenting cells that infiltrate arterial walls and may destabilize atherosclerotic plaques in cardiov ... | 2010 | 21523219 |
| diabetes, periodontitis, and the subgingival microbiota. | both type 1 and type 2 diabetes have been associated with increased severity of periodontal disease for many years. more recently, the impact of periodontal disease on glycaemic control has been investigated. the role of the oral microbiota in this two-way relationship is at this stage unknown. further studies, of a longitudinal nature and investigating a wider array of bacterial species, are required in order to conclusively determine if there is a difference in the oral microbiota of diabetics ... | 2010 | 21523215 |
| bacterial diversity in persistent periapical lesions on root-filled teeth. | the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods. | 2009 | 21523213 |
| distinctive features of the microbiota associated with different forms of apical periodontitis. | microorganisms infecting the dental root canal system play an unequivocal role as causative agents of apical periodontitis. although fungi, archaea, and viruses have been found in association with some forms of apical periodontitis, bacteria are the main microbial etiologic agents of this disease. bacteria colonizing the root canal are usually organized in communities similar to biofilm structures. culture and molecular biology technologies have demonstrated that the endodontic bacterial communi ... | 2009 | 21523208 |
| inhibition of host- and bacteria-derived proteinases by natural anthocyanins. | santos j, la vd, bergeron c, grenier d. inhibition of host- and bacteria-derived proteinases by natural anthocyanins. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01372.x. © 2011 john wiley & sons a/s background and objectives: host- and bacteria-derived proteinases are considered to play critical roles in periodontitis progression. this study investigated the ability of a blackcurrant extract and its major anthocyanins (cyanidin-3-o-glucoside, cyanidin-3-o-rutinoside and delphinidin-3-o ... | 2011 | 21517858 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| pathologic crossroads: cardio-vascular diseases, periodontal diseases and calcium antagonists. | during the last decennium a more focused attention has been directed to the presence of chronic inflammation in cardiovascular diseases (cvd), but mainly to the high impact that this one has in generating and fastening the atherosclerotic process. | 2011 | 21505569 |
| gingival tissue transcriptomes in experimental gingivitis. | jönsson d, ramberg p, demmer rt, kebschull m, dahlén g, papapanou pn. gingival tissue transcriptomes in experimental gingivitis. j clin periodontol 2011; doi: 10.1111/j.1600-051x.2011.01719.x. abstract: aims: we investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. material and methods: twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by deb ... | 2011 | 21501207 |
| a novel approach in the use of subgingival controlled-release delivery of chlorhexidine in chronic periodontitis: a randomized clinical trial. | background: we aimed to analyze clinical, microbiological, and serological effects of chlorhexidine (chx) chips used as subgingival controlled-release-delivery-device before and immediately after scaling and root-planing (srp). methods: 24 patients presenting with at least 12 teeth with ppd ≥5 mm and bop were assigned in test or control groups. after prophylaxis, chx chips (test) or placebo chips (control) were placed in pockets with ppd ≥5mm. ten days later srp was performed in all teeth with p ... | 2011 | 21491990 |
| microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. | uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ... | 2011 | 21488936 |
| oral health behaviors, periodontal disease and pathogens in preeclampsia: a case-control study in korea. | background: the aim of this study was to confirm the association among oral health behaviors, periodontitis and preeclampsia in korean women. methods: this study was designed as a case-control study. sixteen preeclamptic and forty eight non-preeclamptic women after delivery were included in this study from november 2007 to january 2010. information on demographics, health behaviors, obstetric and systemic disease that may influence periodontal condition and preeclampsia was collected. full mouth ... | 2011 | 21486182 |
| comparison of real-time pcr assays for detection of pathogenic leptospira spp. in blood and identification of variations in target sequences. | leptospirosis is considered an underdiagnosed disease. although several pcr-based methods are currently in use, there is little information on their comparability. in this study, four quantitative real-time pcr (qpcr) assays (sybr green and taqman chemistries) targeting the secy, lfb1, and lipl32 genes were evaluated as diagnostic assays. in our hands, these assays can detect between 10(2) and 10(3) bacteria/ml of pure culture, whole-blood, plasma, and serum samples. in three independent experim ... | 2011 | 21471336 |
| molecular signaling mechanisms of the periopathogen, treponema denticola. | in the healthy subgingiva, oral treponemes account for a small percentage of the total bacteria. however, in diseased periodontal pockets, treponemes thrive and become a dominant component of the bacterial population. oral treponemes are uniquely adept at capitalizing on the environmental conditions that develop with periodontal disease. the molecular basis of adaptive responses of oral treponemes is just beginning to be investigated and defined. the completion of several treponeme genome sequen ... | 2011 | 21447698 |
| molecular evolution of urea amidolyase and urea carboxylase in fungi. | urea amidolyase breaks down urea into ammonia and carbon dioxide in a two-step process, while another enzyme, urease, does this in a one step-process. urea amidolyase has been found only in some fungal species among eukaryotes. it contains two major domains: the amidase and urea carboxylase domains. a shorter form of urea amidolyase is known as urea carboxylase and has no amidase domain. eukaryotic urea carboxylase has been found only in several fungal species and green algae. in order to elucid ... | 2011 | 21447149 |
| a prospective cohort study of periodontal disease measures and cardiovascular disease markers in hiv-infected adults. | abstract the determinants of hiv-associated cardiovascular disease (cvd) are not well understood. periodontal disease (pd) has been linked to cvd but this connection has not been examined in hiv infection. we followed a cohort of hiv-infected adults to ascertain whether pd was associated with carotid artery intima media thickness (imt) and brachial artery flow-mediated dilation (fmd). we performed a longitudinal observational study of hiv-infected adults on haart for <2 years with no known heart ... | 2011 | 21443451 |
| changes in the subgingival biofilm composition after coronally positioned flap. | this study evaluated the effects of coronally positioned flap (cpf) on the subgingival biofilm composition. | 2011 | 21437473 |
| genomes of the most dangerous epidemic bacteria have a virulence repertoire characterized by fewer genes but more toxin-antitoxin modules. | we conducted a comparative genomic study based on a neutral approach to identify genome specificities associated with the virulence capacity of pathogenic bacteria. we also determined whether virulence is dictated by rules, or if it is the result of individual evolutionary histories. we systematically compared the genomes of the 12 most dangerous pandemic bacteria for humans ("bad bugs") to their closest non-epidemic related species ("controls"). | 2011 | 21437250 |
| [study of putative periodontal pathogens colonies in type 2 diabetes with chronic periodontitis]. | ppurpose: to study the permanent planting of periodontal pathogens in the subgingival plaque of periodontitis patients with type 2 diabetes, and evaluate role of periodontal pathogens in chronic periodontitis and type 2 diabetes. | 2010 | 21431261 |
| ovine pedomics: the first study of the ovine foot 16s rrna-based microbiome. | we report the first study of the bacterial microbiome of ovine interdigital skin based on 16s rrna by pyrosequencing and conventional cloning with sanger-sequencing. three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. the sheep were classified as having either healthy interdigital skin (h) and interdigital dermatitis (id) or virulent footrot ... | 2011 | 21430786 |
| hgp44 induces protection against porphyromonas gingivalis-induced alveolar bone loss in mice. | the protective effect of dna vaccines expressing the arg-gingipain a domain against bone loss induced by porphyromonas gingivalis infection was investigated in a murine model. phgp44, which expresses the 44-kda adhesion/hemagglutinin domain of arg-gingipain a, prevented p. gingivalis-induced alveolar bone loss. the results indicate that phgp44 could be a candidate antigen for a vaccine against p. gingivalis infection. | 2011 | 21430125 |
| new insights into the emerging role of oral spirochaetes in periodontal disease. | spirochaetes are prominent in the polymicrobial infections that cause periodontal diseases. periodontitis is a chronic inflammatory condition of the periodontium, characterized by proinflammatory soft tissue damage and alveolar bone loss. treponema denticola is the most well-understood oral spirochaete, expressing a wealth of virulence factors that mediate tissue penetration and destruction as well as evasion of host immune responses. this review focuses on emerging knowledge of virulence mechan ... | 2011 | 21414084 |