Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| phenotypic and genotypic characterization of sorbitol-negative or slow-fermenting (suspected o157) escherichia coli isolated from milk samples in lombardy region. | to investigate phenotypic and genotypic aspects of sorbitol-negative or slow-fermenting escherichia coli, suspected to belong to o157 serogroup, isolated in italy. | 2005 | 15892748 |
| immunorelevant gene expression in lps-challenged bovine mammary epithelial cells. | infection of the bovine mammary gland, in addition to causing animal distress, is a major economic burden of the dairy industry. greater understanding of the initial host response to infection may lead to more accurate selection of resistant animals or to novel prophylactic or therapeutic intervention strategies. the epithelial cell plays a role in the host response by alerting the immune system to the infection and providing a signal as to where the infection is located. to understand this proc ... | 2005 | 15876684 |
| characterization of shiga toxin-producing escherichia coli isolated from aquatic environments. | this study reports the phenotypic and genotypic characterization of 144 shiga toxin-producing escherichia coli (stec) strains isolated from urban sewage and animal wastewaters using a shiga toxin 2 gene variant (stx(2))-specific dna colony hybridization method. all the strains were classified as e. coli and belonged to 34 different serotypes, some of which had not been previously reported to carry the stx(2) genes (o8:h31, o89:h19, o166:h21 and o181:h20). five stx(2) subtypes (stx(2), stx(2c), s ... | 2005 | 15869962 |
| expression of soluble, biologically active recombinant human endostatin in escherichia coli. | endostatin, a 20kda c-terminal fragment of collagen xviii, is a potent anti-angiogenic protein and inhibitor of tumor growth. recombinant endostatin was prepared from escherichia coli deposited as insoluble, inactive inclusion bodies. in the present study, we produced soluble and biologically active recombinant human endostatin (rhendostatin) in e. coli by employing both co-expression of the molecular chaperones and lower temperature fermentation. two groups of chaperones trigger factor and groe ... | 2005 | 15866710 |
| a longitudinal study of escherichia coli o157 in cattle of a dutch dairy farm and in the farm environment. | from july 1999 till november 2000, a longitudinal study was conducted on a dairy farm in the netherlands to study within herd prevalence and types of verocytotoxin producing escherichia coli (vtec) of serogroup o157 over time, and determine environmental reservoirs and possible transmission routes. faeces, blood, milk and environmental samples were collected 14 times with intervals varying from 4 to 10 weeks during the study period. faecal samples were selectively cultured for escherichia coli o ... | 2005 | 15863278 |
| novel preharvest strategies involving the use of experimental chlorate preparations and nitro-based compounds to prevent colonization of food-producing animals by foodborne pathogens. | foodborne diseases caused by enterohemorrhagic escherichia coli, salmonella, and campylobacter species are of public health and economic significance. shedding of these pathogens during production and slaughter are risks for contamination of products for human consumption. consequently, strategies are sought to prevent or reduce the carriage of these pathogens in food animals before slaughter. experimental products containing chlorate salts have been proven efficacious in reducing concentrations ... | 2005 | 15844824 |
| effects of using retention-pond water for dust abatement on performance of feedlot steers and carriage of escherichia coli o157 and salmonella spp. | to evaluate the effects of using retention-pond water for dust abatement on performance of feedlot steers and carriage of escherichia coli o157 and salmonella spp. | 2005 | 15844433 |
| escherichia coli o157:h7 outbreak associated with consumption of ground beef, june-july 2002. | a case-control and environmental study tested the hypothesis that purchasing and eating ground beef from a specific source was the cause of a cluster of cases of hemolytic uremic syndrome (hus) and escherichia coli (e. coli) o157:h7 gastroenteritis. | 2005 | 15842119 |
| characterization of class 1 integrons-mediated antibiotic resistance among calf pathogenic escherichia coli. | escherichia coli isolates from calf diarrhea cases (n=22) in the beijing surrounding region in china were characterized for disease serotype, virulence factors, antimicrobial susceptibility pattern and class 1 integrons. 59% (n=13) of the isolates were positive for the int i1 gene. the presence and genetic content of class 1 integrons in 13 e. coli isolates were examined by pcr and sequencing. sequencing analysis revealed six gene cassettes, which encoded resistance to trimethoprim (dfra1, dfra1 ... | 2005 | 15837385 |
| prevalence and numbers of escherichia coli o157 on bovine hides at a beef slaughter plant. | in this study, we investigated the prevalence and numbers of escherichia coli o157 on bovine hides. samples (n = 1,500) were collected over a 17-month period (30 samples per week) by sponge swabbing approximately 122-cm2 areas of the bovine rump of slaughtered cattle at an early stage of carcass processing (first legging). sponge samples (n = 1,500) were stomached in buffered peptone water supplemented with novobiocin, directly plated on sorbitol macconkey with cefixime tellurite (smac-ct), enri ... | 2005 | 15830653 |
| a novel dimeric structure of the riml nalpha-acetyltransferase from salmonella typhimurium. | riml is responsible for converting the prokaryotic ribosomal protein from l12 to l7 by acetylation of its n-terminal amino group. we demonstrate that purified riml is capable of posttranslationally acetylating l12, exhibiting a v(max) of 21 min(-1). we have also determined the apostructure of riml from salmonella typhimurium and its complex with coenzyme a, revealing a homodimeric oligomer with structural similarity to other gcn5-related n-acetyltransferase superfamily members. a large central t ... | 2005 | 15817456 |
| the prevalence of shiga toxin-producing escherichia coli in domestic animals and food in serbia. | faecal samples of 2660 domestic animals from 116 farms and 956 samples of food were examined for the presence of shiga toxin-producing escherichia coli (stec). stec was recovered from 126 (15.3%) cattle, 135 (11.3%) pigs, 135 (66.8%) sheep, 31 (73.8%) goats, 4 (1%) chicken, and 15 (1.6%) food samples. of all stec isolates, 21.5, 25.8 and 15% produced enterohaemolysin, alpha-haemolysin, and aerobactin respectively, 1.6% displayed localized adherence (la) to hep-2 cells, 27.6% were sorbitol negati ... | 2005 | 15816163 |
| prevalence of verotoxin-producing escherichia coli (vtec) 0157 in swedish dairy herds. | a prevalence study of verotoxin-producing escherichia coli o157 (vtec o157) was performed in 371 randomly selected dairy herds distributed throughout sweden. faecal and manure samples were collected and analysed by immunomagnetic separation and culturing. data were recorded for each herd regarding herd size, age of sampled animals and whether, in addition to cattle, the farm kept other animals. vtec o157 was isolated from 33 (8.9%) of the 371 investigated herds. the prevalence was higher (23.3%) ... | 2005 | 15816162 |
| prevalence and genetic diversity of campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area. | water samples were taken systematically from a 100-km2 area of mainly dairy farmland in northwestern england and examined for campylobacter spp. pulsed-field gel electrophoresis-restriction fragment length polymorphism (pfge-rflp) and flaa strain typing of campylobacter jejuni and campylobacter coli isolates were done. data on the water source and the adjacent environment were recorded and examined as explanatory variables. campylobacter spp. were isolated from 40.5% (n = 119) of the water sampl ... | 2005 | 15812015 |
| inactivation of escherichia coli o157:h7 in packaged ground beef by allyl isothiocyanate. | commercial allyl isothiocyanate (ait) was examined for its ability to reduce numbers of escherichia coli o157:h7 inoculated in fresh ground beef packaged under nitrogen and stored refrigerated or frozen. a five-strain cocktail of e. coli o157:h7 containing 3 or 6 log10 cfu/g was inoculated into 100 g ground beef and formed into 10x1-cm patties. a 10-cm diameter filter paper disk treated with ait suspended in sterile corn oil was placed on top of a single patty. one patty and paper disk were plac ... | 2005 | 15808361 |
| food safety and inspection service regulatory testing program for escherichia coli o157:h7 in raw ground beef. | we analyzed raw ground beef testing data to determine whether a decrease in the rate of escherichia coli o157:h7-positive raw ground beef samples has occurred since the inception of food safety and inspection service (u.s. department of agriculture) regulatory actions and microbiological testing concerning this commodity and pathogen. a main effects log-linear poisson regression model was constructed to evaluate the association between fiscal year and the rate of e. coli o157:h7-positive raw gro ... | 2005 | 15771167 |
| dynamic profiles of dna: analysis of cap- and lexa protein-binding regions with endonucleases. | we examined ssdna and dsdna containing a cap-binding region or a lexa protein-binding region in the reca gene of escherichia coli with endonucleases (e. coli endonuclease i and bovine dnase i) to deduce the structural conformation of dsdna as well as ssdna. each nuclease produced its own cleavage pattern. some cleavages occurred at common sites in ssdna and dsdna. the common cleavage sites for endonuclease i included ones that have been inferred to be functionally important. efficient cleavage o ... | 2005 | 15767782 |
| identification by site-directed mutagenesis and chemical modification of three vicinal cysteine residues in rat mitochondrial carnitine/acylcarnitine transporter. | the proximity of the cys residues present in the mitochondrial rat carnitine/acylcarnitine carrier (cac) primary structure was studied by using site-directed mutagenesis in combination with chemical modification. cac mutants, in which one or more cys residues had been replaced with ser, were overexpressed in escherichia coli and reconstituted into liposomes. the effect of sh oxidizing, cross-linking, and coordinating reagents was evaluated on the carnitine/carnitine exchange catalyzed by the rec ... | 2005 | 15757911 |
| n-terminal deletion of the gamma subunit affects the stabilization and activity of chloroplast atp synthase. | five truncation mutants of chloroplast atp synthase gamma subunit from spinach (spinacia oleracea) lacking 8, 12, 16, 20 or 60 n-terminal amino acids were generated by pcr by a mutagenesis method. the recombinant gamma genes were overexpressed in escherichia coli and assembled with alphabeta subunits into a native complex. the wild-type (wt) alphabetagamma assembly i.e. alphabetagammawt exhibited high (mg2+)-dependent and (ca2+)-dependent atp hydrolytic activity. deletions of eight residues of t ... | 2005 | 15752355 |
| antimicrobial-resistant and extraintestinal pathogenic escherichia coli in retail foods. | extraintestinal escherichia coli infections are associated with specialized extraintestinal pathogenic e. coli (expec) strains and, increasingly, with antimicrobial resistance. the food supply may disseminate expec and antimicrobial-resistant e. coli. | 2005 | 15747237 |
| comparison of diversities of escherichia coli o157 shed from a cohort of spring-born beef calves at pasture and in housing. | a cohort of spring-born beef calves demonstrated limited genetic and phenotypic diversity of escherichia coli o157 when kept in a state of isolation. despite this, there was a difference in the pulsed-field gel electrophoresis and phage types of isolates shed by cattle at pasture compared with those shed by the same cattle when weaned and housed. | 2005 | 15746371 |
| antimicrobial susceptibility of escherichia coli isolates from dairy farms using organic versus conventional production methods. | to compare antimicrobial susceptibility patterns of escherichia coli isolates cultured from fecal samples from cows and calves on dairy farms that used organic (ie, no or severely limited antimicrobial use) versus conventional production methods. | 2005 | 15742702 |
| characterization of biv env core: implication for mechanism of biv-mediated cell fusion. | entry of lentiviruses, such as human immunodeficiency virus type 1 (hiv-1) and simian immunodeficiency virus (siv), requires folding of two heptad repeat regions (hr1 and hr2) of gp41 into a trimer-of-hairpins, which subsequently brings virus and cell membrane into fusion. this motif is a generalized feature of viral fusion proteins and has been exploited in generating antiviral fusion agents. in the present paper, we report structural characters of env protein from another lentivirus, bovine im ... | 2005 | 15737628 |
| investigation of domestic animals and pets as a reservoir for intimin- (eae) gene positive escherichia coli types. | domestic animals belonging to seven different species (cattle, sheep, dogs, cats, pigs, chicken and goats) were investigated as natural reservoirs for attaching and effacing escherichia coli (aeec). for this, 2165 e. coli strains from faeces of 803 animals were examined for the presence of the intimin -(eae) gene as a characteristic of aeec strains. ten percent of the animals were found to excrete aeec, most frequently found in sheep (19.2%) and pigs (17.6), followed by cattle (10.4%), dogs (7.2 ... | 2005 | 15737477 |
| effects of diet energy density and milking frequency in early lactation on tumor necrosis factor-alpha responsiveness in dairy cows. | a whole blood stimulation assay (wba) with escherichia coli lipopolysaccharide (lps) and an enzyme-linked immunosorbent assay (elisa) were established to measure the production of tumor necrosis factor-alpha (tnf-alpha) in bovine plasma. the assays were used to study the effect of time around parturition, and diet energy density, and milking frequency on tnf-alpha responsiveness of dairy cows in early lactation. forty cows were included in a 2 x 2 factorial block design. one factor was high (h) ... | 2005 | 15734538 |
| identification of enterohemorrhagic escherichia coli o26:h- genes required for intestinal colonization in calves. | enterohemorrhagic escherichia coli (ehec) infections in humans are an important public health problem and are commonly acquired via contact with ruminant feces. the serogroups that are predominantly associated with human infection in the united states and europe are o157 and o26. serotypes o157:h7 and o26:h- differ in their virulence and tissue tropism in calves and therefore may colonize calves by distinct mechanisms. the mechanisms underlying ehec intestinal colonization and pathogenesis are p ... | 2005 | 15731074 |
| isolation of escherichia coli o157 from human and bovine faeces in the urbino area, italy. | we examined 476 faecal samples from subjects aged from 0 to >60 years, 283 with diarrhoea and 193 with illnesses involving other sites or clinically healthy, and 154 samples of faeces of healthy cattle, in order to define the diffusion of e. coli o157 in the urbino area. the samples were seeded by both direct streaking onto cefixime tellurite sorbitol mac conkey agar (ct-smac) and previous enrichment in cefixime tellurite tryptone soya broth for human specimens and in cefixime vancomicin trypton ... | 2004 | 15729839 |
| dose dependency and individual variability in selected clinical, haematological and blood biochemical responses after systemic lipopolysaccharide challenge in cattle. | previous studies have noted that susceptibility to systemic lipopolysaccharide (lps) exposure seems to differ between individual cows. however, to date inter-individual variation in the response to intravenous injection of lps has been reported only as an empirical finding, and its existence or extent has never been backed up by statistical analyses. the aim of the present study was therefore to investigate the dose-dependency of clinical, haematological and blood biochemical responses to intrav ... | 2005 | 15720970 |
| effect of escherichia coli wild type or its derivative with high nitrite reductase activity on in vitro ruminal methanogenesis and nitrate/nitrite reduction. | the effects of two kinds of escherichia coli strains, wild-type e. coli w3110 or e. coli nir-ptac, which has enhanced nitrite reduction activity, on in vitro ch4 production and nitrate and nitrite reduction in cultures of mixed ruminal microorganisms was investigated using continuous incubation systems. escherichia coli nir-ptac, a derivative of wild-type e. coli w3110, was constructed by replacing self promoter of nir bd operon encoding subunits of nitrite reductase in e. coli w3110 by tac prom ... | 2005 | 15705761 |
| persistence of escherichia coli o157 isolates on bovine farms in england and wales. | we performed pulsed-field gel electrophoresis on escherichia coli o157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 months. the cohort animals were repeatedly sampled, and for some of these, the same clones were obtained on sampling occasions separated by as much as 8 months. | 2005 | 15695700 |
| mass spectrometric analysis of affinity-captured proteins on a dendrimer-based immunosensing surface: investigation of on-chip proteolytic digestion. | the monolayer of fourth-generation poly(amidoamine) dendrimers was adopted to construct the immunoaffinity surface of an antibody layer. the antibody layer as a bait on the dendrimer monolayer was found to result in high binding capacity of antigenic proteins and a reliable detection. the affinity-captured protein at the immunosensing surface was subjected to direct on-chip tryptic digestion, and the resulting proteolytic peptides were analyzed by using matrix-assisted laser desorption/ionizatio ... | 2005 | 15691510 |
| myositis, lameness, and recumbency after use of water-in-oil adjuvanted vaccines in near-term beef cattle. | a producer administered 2 us department of agriculture-licensed adjuvanted veterinary vaccines (inactivated bovine rotavirus-coronavirus vaccine; clostridium perfringens type c-escherichia coli bacterin-toxoid) into muscles of the left and right hips of 469 pregnant beef cows. within 24 hours, 5 cattle were recumbent, and another 2 had non-weight bearing pelvic limb lameness (1.5% affected; 7/469). during the next 10 days, 50% of the herd developed firm swellings up to 24 cm in vaccination sites ... | 2005 | 15690947 |
| shiga toxin-producing escherichia coli: pre- and postharvest control measures to ensure safety of dairy cattle products. | the large number of cases of human illness caused by shiga toxin-producing escherichia coli (stec) worldwide has raised safety concerns for foods of bovine origin. these human illnesses include diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura. severe cases end with chronic renal failure, chronic nervous system deficiencies, and death. over 100 stec serotypes, including e. coli o157:h7, are known to cause these illnesses and to be shed in cattle fe ... | 2005 | 15690827 |
| prevalence and associations between bacterial isolates from dry mammary glands of dairy cows. | to assess the prevalence and patterns of bacterial isolates, cultures were made from the dry mammary glands of dairy cows in six commercial dairy herds in the uk. milk samples were taken from all four quarters of 480 cows at drying off and at weekly intervals from 14 days before to seven days after calving. a major mastitis pathogen was isolated from at least one quarter of 220 (45.8 per cent) of the cows and from more than one quarter of 90 (18.8 per cent) of them. during the late dry to calvin ... | 2005 | 15689035 |
| virulence genes and intimin types of shiga-toxin-producing escherichia coli isolated from cattle and beef products in argentina. | a total of 153 shiga-toxin-producing escherichia coli (stec) isolates from feces of cattle and beef products (hamburgers and ground beef) in argentina were characterized in this study. pcr showed that 22 (14%) isolates carried stx1 genes, 113 (74%) possessed stx2 genes and 18 (12%) both stx1 and stx2. intimin (eae), enterohemolysin (ehxa), and stec autoagglutinating adhesin (saa) virulence genes were detected in 36 (24%), 70 (46%) and in 34 (22%) of the isolates, respectively. none of 34 saa-pos ... | 2004 | 15666247 |
| immunoreactivity of the mycobacterium avium subsp. paratuberculosis 19-kda lipoprotein. | the mycobacterium tuberculosis 19-kda lipoprotein has been reported to stimulate both t and b cell responses as well as induce a number of th1 cytokines. in order to evaluate the mycobacterium avium subsp. paratuberculosis (m. avium subsp. paratuberculosis) 19-kda lipoprotein as an immunomodulator in cattle with johne's disease, the gene encoding the 19-kda protein (map0261c) was analyzed. | 2005 | 15663791 |
| kinetics of local and systemic isoforms of serum amyloid a in bovine mastitic milk. | the aim of the present study was to characterise the serum amyloid a (saa) response to intramammary inoculation of escherichia coli and to examine the distribution of hepatically and extrahepatically produced saa isoforms in plasma and milk from cows with mastitis. milk and plasma saa concentrations were determined before and after experimental induction of e. coli mastitis in six dairy cows. the milk saa response was characterised by low or undetectable levels before inoculation, very rapid and ... | 2005 | 15661328 |
| escherichia coli o157:h7 infections associated with ground beef from a u.s. military installation--okinawa, japan, february 2004. | in february 2004, the okinawa prefectural chubu health center (ochc) and the okinawa prefectural institute of health and environment (oihe), japan, investigated three cases of escherichia coli o157:h7 infection in a japanese family associated with eating ground beef. public health officials from multiple agencies in japan and the united states collaborated on this investigation, which resulted in a voluntary recall of approximately 90,000 pounds of frozen ground beef in the united states and at ... | 2005 | 15660018 |
| characterization and functional study of antrodia camphorata lipopolysaccharide. | lipopolysaccharide (lps) is a highly proinflammatory molecule isolated from bacteria. this study demonstrated the existence of lps in a medicinal fungus, antrodia camphorata. because no lps had been identified in any fungus organism, the purification of lps from a. camphorata was attempted. lpss from six strains of a. camphorata (35396, 35398, 35716, b71, b85, and b86) were isolated. chemical and functional properties were investigated on the fungus lps. compositional analysis revealed that sorb ... | 2005 | 15656690 |
| possible animal origin of human-associated, multidrug-resistant, uropathogenic escherichia coli. | the multistate occurrence of cases of urinary tract infection (uti) caused by trimethoprim-sulfamethoxazole (tmp-smz)-resistant escherichia coli strains belonging to a single clonal group (designated as clonal group a [cga]) in the united states has raised an intriguing hypothesis that these infections may have been spread by contaminated food products. the present study attempted to determine if cga strains could be traced to food animals. | 2004 | 15655743 |
| prevalence of shiga toxin-producing escherichia coli in dairy cattle and their products. | the main objective of this review was to assess the role of dairy cattle and their products in human infections with shiga toxin-producing escherichia coli (stec). a large number of stec strains (e.g., members of the serogroups o26, o91, o103, o111, o118, o145, and o166) have caused major outbreaks and sporadic cases of human illnesses that have ranged from mild diarrhea to the life-threatening hemolytic uremic syndrome. these illnesses were traced to o157 and non-o157 stec. in most cases, stec ... | 2005 | 15653509 |
| induction of thymus-derived gammadelta t cells by escherichia coli enterotoxin b subunit in peritoneal cavities of mice. | we examined the activation of intraperitoneal t cells in balb/c mice by the escherichia coli enterotoxin b subunit, which induced a specific th2 type of t-cell response to intraperitoneally coadministered bovine immunoglobulin g. the numbers of both gammadelta and alphabeta t cells increased significantly after intraperitoneal administration of the b subunit in a time-dependent manner; these numbers were not affected by the b-subunit g33d mutant, which is defective in gm1 ganglioside-binding abi ... | 2005 | 15643001 |
| effect of sand and sawdust bedding materials on the fecal prevalence of escherichia coli o157:h7 in dairy cows. | farm management practices that reduce the prevalence of food-borne pathogens in live animals are predicted to enhance food safety. to ascertain the potential role of livestock bedding in the ecology and epidemiology of escherichia coli o157:h7 on farms, the survival of this pathogen in used-sand and used-sawdust dairy cow bedding was determined. additionally, a longitudinal study of mature dairy cattle housed on 20 commercial dairy farms was conducted to compare the prevalence of e. coli o157:h7 ... | 2005 | 15640205 |
| rectal carriage of enterohemorrhagic escherichia coli o157 in slaughtered cattle. | escherichia coli o157:h7 is an important cause of diarrhea, hemorrhagic colitis, and potentially fatal human illness. cattle are considered a primary reservoir of infection, and recent experimental evidence has indicated that the terminal rectum is the principal site of bacterial carriage. to test this finding in naturally colonized animals, intact rectum samples from 267 cattle in 24 separate lots were obtained immediately after slaughter, and fecal material and mucosal surfaces were cultured f ... | 2005 | 15640175 |
| coliform and pathologic bacteria in sierra nevada national forest wilderness area lakes and streams. | to analyze backcountry-area water quality in us department of agriculture forest service-designated wilderness areas for the presence of coliform and potentially pathogenic bacteria. | 2004 | 15636374 |
| horizontal transmission of escherichia coli o157:h7 during cattle housing. | ruminant livestock, particularly cattle, is considered the primary reservoir of escherichia coli o157:h7. this study examines the transmission of e. coli o157:h7 within groups of cattle during winter housing. holstein friesian steers were grouped in six pens of five animals. an animal inoculated with and proven to be shedding a marked strain of e. coli o157: h7 was introduced into each pen. fecal (rectal swabs) and hide samples (900 cm2 from the right rump) were taken from the 36 animals through ... | 2004 | 15633666 |
| attaching-effacing bacteria in animals. | enteric bacteria with a demonstrable or potential ability to form attaching-effacing lesions, so-called attaching-effacing (ae) bacteria, have been found in the intestinal tracts of a wide variety of warm-blooded animal species, including man. in some host species, for example cattle, pigs, rabbits and human beings, attaching-effacing escherichia coli (aeec) have an established role as enteropathogens. in other host species, ae bacteria are of less certain significance. with continuing advances ... | 2005 | 15629476 |
| [hazard evaluation of livestock-derived, verotoxin-producing escherichia coli by enterohemolysin production assay and eaea gene detection]. | we examined enterohemolysin (ehly) production, and detected the hlya gene and the eaea gene for the intestinal mucosal adherence factor intimin in 131 strains of human-derived verotoxin-producing escherichia coli (vtec) and 140 strains of livestock (cattle and swine) -derived vtec to evaluate their hazards to humans. the hlya gene was confirmed in 98.5% of human-derived, in 50.5% of cattle-derived, and in 10.3% of swine-derived vtec strains. ehly-positive rates were 96.2-97.7%, 45.9-55.0%, and 1 ... | 2004 | 15628530 |
| role of s fimbriae in escherichia coli k1 binding to brain microvascular endothelial cells in vitro and penetration into the central nervous system in vivo. | bacterial binding to host cell surface is considered an important initial step in the pathogenesis of many infectious diseases including meningitis. previous studies using a laboratory escherichia coli (e. coli) strain hb101 possessing a recombinant plasmid carrying the cloned s fimbriae gene cluster have shown that s fimbriae are the major contributor to binding to bovine brain microvascular endothelial cells (bmec) for hb101. our present study, however, revealed that s fimbriae did not play a ... | 2004 | 15619424 |
| bile-induced dna damage in salmonella enterica. | in the absence of dna adenine methylase, growth of salmonella enterica serovar typhimurium is inhibited by bile. mutations in any of the muth, mutl, and muts genes suppress bile sensitivity in a dam(-) background, indicating that an active muthls system renders dam(-) mutants bile sensitive. however, inactivation of the muthls system does not cause bile sensitivity. an analogy with escherichia coli, in which the muthls system sensitizes dam(-) mutants to dna-injuring agents, suggested that bile ... | 2004 | 15611156 |
| serotypes and virulence markers of shiga toxin-producing escherichia coli (stec) isolated from dairy cattle in são paulo state, brazil. | in order to determine the occurrence, serotypes and virulence markers of shiga toxin-producing escherichia coli (stec) strains, 153 fecal samples of cattle randomly selected from six dairy farms in sao paulo state, brazil, were examined for shiga toxin (stx) production by the vero cell assay. feces were directly streaked onto macconkey sorbitol agar and incubated at 37 degrees c overnight. sorbitol-negative colonies (maximum 20) and up to 10 sorbitol-positive colonies from each plate were subcul ... | 2004 | 15607081 |
| endogenous glucocorticoids modulate neutrophil function in a murine model of haemolytic uraemic syndrome. | haemolytic uraemic syndrome (hus) is caused by shiga-toxin-producing escherichia coli (stec). although, shiga toxin type 2 (stx2) is responsible for the renal pathogenesis observed in patients, the inflammatory response, including cytokines and polymorphonuclear neutrophils (pmn), plays a key role in the development of hus. previously, we demonstrated that stx2 injection generates an anti-inflammatory reaction characterized by endogenous glucocorticoid (gc) secretion, which attenuates hus severi ... | 2005 | 15606615 |
| high level production of bovine angiogenin in e. coli by an efficient refolding procedure. | recombinant bovine angiogenin (rbang) was expressed in e. coli at up to 30% of total cell proteins but was produced as inclusion bodies. by investigating the effect of various factors on the refolding yield, we obtained about 60% refolding. after chromatographic purification, about 60 mg purified angiogenin was obtained from 1 l culture. the purified recombinant bovine angiogenin was identical to native bovine angiogenin (nbang) obtained from cow's milk. our approach is highly efficient and can ... | 2004 | 15604787 |
| the association of herd milk production and management with a return-over-feed index in ontario dairy herds. | associations of herd milk production and management variables to a return-over-feed (rof) herd profit index were examined among 95 dairy farms. the rof index is derived from 2 important determinants of profit on dairy farms: milk income and feed cost. all producers were participants in the dairy herd improvement (dhi) rof program in ontario, canada during 2002. nutrition, housing, health, and other management data were collected through a phone survey of herd managers. herd milk production, milk ... | 2005 | 15591408 |
| comparative efficacy of local and systemic antibiotic treatment in lactating cows with clinical mastitis. | the intramuscular administration of penethamate hydriodide over 3 consecutive days and the intramammary administration of an ampicillin/cloxacillin combination were compared in lactating cows suffering from infectious clinical mastitis in one quarter, through an open, randomized, controlled multicenter field trial. clinical examinations were carried out on d 1 (immediately before treatment), 3, 8, 17, and 22. milk samples were taken from affected quarters for bacteriological analysis on d 1, 17, ... | 2005 | 15591371 |
| clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. | bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ... | 2005 | 15589277 |
| [the prevalence of antimicrobial susceptibility of veterinary pathogens isolated from cattle and pigs: national antibiotic resistance monitoring 2002/2003 of the bvl]. | the national antimicrobial resistance monitoring determines the current quantitative resistance level of life-stock pathogens, in order to permit the evaluation and surveillance of the distribution of resistances on a valid basis. during the examination period from june 2002 to july 2003, a total of 1849 pathogens was collected, following a representative german-wide pattern in collaboration with 29 laboratories. the selection of examined bacterial strains included different specimen causing res ... | 2004 | 15584430 |
| development of a rapid pcr method using the insertion sequence is1203 for genotyping shiga toxin-producing escherichia coli o157. | we developed a rapid pcr method utilizing the diversity of the insertion site is1203 for genotyping shiga toxin-producing escherichia coli (stec) o157 (is1203 pcr typing). dna fragments digested by pvuii, which cut is1203 at one site, were ligated with themselves and detected by pcr with outward-facing primer pairs for is1203. to minimize nonspecific bands, nested pcr was also performed. two fingerprinting patterns produced from the upstream or downstream regions of is1203 were obtained within 1 ... | 2004 | 15583266 |
| factors associated with the presence of escherichia coli o157 in feedlot-cattle water and feed in the midwestern usa. | our objective was to generate hypotheses about associations between management, climate, and the presence of escherichia coli o157 in feedlot-cattle water tanks and in feedlot-cattle feed. water samples from 710 tanks on 73 feedlots, and feed-samples from a subset of 504 pens on 54 feedlots, in four us states were tested for e. coli o157. management and climate factors were ascertained by survey and observation. escherichia coli o157 were isolated from 13% of the water tanks and at least one wat ... | 2004 | 15579344 |
| methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies. | the determination of structures for proteins that control the eukaryotic cell cycle by nuclear magnetic resonance (nmr) spectroscopy and x-ray crystallography has made a significant contribution to our understanding of the molecular mechanisms that control cell cycle progression. cdk2 has proved particularly tractable to structural analysis, and cdk2 in complex with various regulatory proteins and in different phosphorylation states provides a paradigm for the control of this important kinase fa ... | 2005 | 15576935 |
| association of escherichia coli o157:h7 with houseflies on a cattle farm. | the ecology of escherichia coli o157:h7 is not well understood. the aims of this study were to determine the prevalence of and characterize e. coli o157:h7 associated with houseflies (hf). musca domestica l. hf (n = 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for e. coli o157:h7 isolation and quantification. the prevalence of e. coli o157:h7 was 2.9 and 1.4% in hf collected from feed bunks and a cattle feed storage shed, respectively. e. ... | 2004 | 15574966 |
| characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens. | phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ... | 2004 | 15574903 |
| nmr structure of the bovine prion protein isolated from healthy calf brains. | nmr structures of recombinant prion proteins from various species expressed in escherichia coli have been solved during the past years, but the fundamental question of the relevancy of these data relative to the naturally occurring forms of the prion protein has not been directly addressed. here, we present a comparison of the cellular form of the bovine prion protein isolated and purified from healthy calf brains without use of detergents, so that it contains the two carbohydrate moieties and t ... | 2004 | 15568016 |
| genetic diversity of escherichia coli recovered from the oral cavity of beef cattle and their relatedness to faecal e. coli. | to determine the genetic diversity of generic escherichia coli recovered from the oral cavities of beef cattle and their relatedness to e. coli isolated from the faeces of cattle during pasture grazing and feedlot finishing. | 2004 | 15548305 |
| viability of milk neutrophils and severity of bovine coliform mastitis. | to study the host-pathogen interactions during escherichia coli mastitis, we first determined whether e. coli infection would change blood and milk polymorphonuclear neutrophil (pmn) chemiluminescence (cl) and viability. we then hypothesized that when e. coli invade the mammary gland, the viable pmn in milk would efficiently phagocytose and destroy e. coli before establishment of infection. we observed that the phagocytosis-dependent and independent cl were closely linked to pmn viability and we ... | 2004 | 15545377 |
| efficacy of an escherichia coli j-5 mutant strain bacterin in the protection of calves from endotoxin disease caused by subcutaneous challenge with endotoxins from escherichia coli. | the purpose of this trial was to examine the potential of a new escherichia (e) coli j-5 mutant strain bacterin to reduce the severity of clinical disease caused by subcutaneous challenge with endotoxins of gram-negative bacteria in calves. day-old to 3-day old calves (n = 40 per study phase) were randomly assigned to either of two treatment groups, i.e. a vaccinated or a placebo group. calves in the vaccinated group received an inactivated bacterin containing a j-5 mutant strain of e. coli via ... | 2004 | 15542194 |
| serum amyloid a and tnf alpha in serum and milk during experimental endotoxin mastitis. | a cross-over study was conducted to investigate the effect of intramammarily infused lipopolysaccharide (lps) on the acute phase reaction in early (el) and in late (ll) lactation. nine cows received intramammary injections of 100 microg of escherichia coli 0111:b4 lps during el and ll. the severity of each cows systemic and local signs and change in milk appearance were recorded and scored throughout the experiment. systemic and local signs were found to be more serious in el cows. tumor necrosi ... | 2004 | 15535955 |
| the sensitivity of escherichia coli strains with k1 surface antigen and rods without this antigen to the bactericidal effect of serum. | the susceptibility of escherichia coli strains with k1 surface antigen (k1+) and rods without this antigen (k1-) to the bactericidal action of normal bovine serum and human normal cord serum was determined. seventy e. coli strains (35 k1+ and 35 k1-) were isolated from urine obtained from children with urinary tract infections. the strains investigated showed variable sensitivity to the bactericidal action of the sera. e. coli k1+ strains were characterized by lower sensitivity to bactericidal e ... | 2004 | 15530015 |
| age-related decline in carriage of ampicillin-resistant escherichia coli in young calves. | the presence of ampicillin-resistant escherichia coli (amp(r) e. coli) in the fecal flora of calves was monitored on a monthly basis in seven cohorts of calves. calves were rapidly colonized by amp(r) e. coli, with peak prevalence in cohort calves observed in the 4 months after the calves were born. the prevalence of calves yielding amp(r) e. coli in cohorts consistently declined to low levels with increasing age of the calves (p < 0.001). | 2004 | 15528566 |
| distribution of escherichia coli o157:h7 within and among cattle operations in pasture-based agricultural areas. | to determine the distribution of escherichia coli o157:h7 in pasture-based cattle production areas. | 2004 | 15524323 |
| escherichia coli o157 cluster evaluation. | we investigated a multistate cluster of escherichia coli o157:h7 isolates; pulsed-field gel electrophoresis subtyping, using a single enzyme, suggested an epidemiologic association. an investigation and additional subtyping, however, did not support the association. confirmating e. coli o157 clusters with two or more restriction endonucleases is necessary before public health resources are allocated to follow-up investigations. | 2004 | 15504278 |
| [relationship between morphological characteristics of the teat duct and prevalence of intramammary infections with streptococcus agalactiae in dairy cows]. | the objective of the study was to determine the relationship between morphological findings of the surface of teat duct particularly the level of ceratosis and the prevalence of intramammary infections (imi). the study was conducted on a commercial dairy herd housing about 3000 lactating dairy cows. we examined 891 quarters in the middle of lactation. duplicate samples of quarter foremilk were collected monthly. the bacteriological status of quarters was determined according to the recommendatio ... | 2004 | 15503535 |
| [cloning and expression of pituitary prolactin gene in ailuropoda melanoleuca]. | the giant panda (ailuropoda melanoleuca) is an endangered species and indigenous to china. it has been proposed that it has a highly specialized reproductive pattern with low fecundity, but little is known about its basic reproductive biology at molecular level. in this study,the pituitary prolactin (prl) cdna of giant panda was amplified by rt-pcr from pituitary total rna and then cloned, sequenced and submitted to genbank (genbank accession no. ay161285). the sequence analysis revealed that th ... | 2004 | 15493147 |
| chaperone activity and prodan binding at the self-associating domain of erythroid spectrin. | spectrin, the major constituent protein of the erythrocyte membrane skeleton, exhibits chaperone activity by preventing the irreversible aggregation of insulin at 25 degrees c and that of alcohol dehydrogenase at 50 degrees c. the dimeric spectrin and the two subunits, alpha-spectrin and beta-spectrin prevent such aggregation appreciably better, 70% in presence of dimeric spectrin at an insulin:spectrin ratio of 1:1, than that in presence of the tetramer of 25%. our results also show that spectr ... | 2004 | 15492010 |
| molecular cloning of bovine cardiac muscle heat-shock protein 70 kda and its phosphorylation by camp-dependent protein kinase in vitro. | the 70-kda heat-shock protein (hsp70) has been cloned and sequenced from bovine cardiac muscle. on the basis of sequence features, the gene corresponds to the cytoplasmic form of hsp70. this cardiac hsp70 cdna clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25 kda. comparison of the amino acid sequence revealed an extensive sequence identity with other species of hsp70. escherichia coli expressed cardiac hsp70 stimulated a 2-fold increas ... | 2004 | 15491140 |
| intermittent and persistent shedding of escherichia coli o157 in cohorts of naturally infected calves. | we conducted two short-term studies of cohorts of naturally infected calves to determine the prevalence and concentrations of escherichia coli o157 shed in faeces. | 2004 | 15479421 |
| optimization of an escherichia coli system for cell-free synthesis of selectively n-labelled proteins for rapid analysis by nmr spectroscopy. | cell-free protein synthesis offers rapid access to proteins that are selectively labelled with [15n]amino acids and suitable for analysis by nmr spectroscopy without chromatographic purification. a system based on an escherichia coli cell extract was optimized with regard to protein yield and minimal usage of 15n-labelled amino acid, and examined for the presence of metabolic by-products which could interfere with the nmr analysis. yields of up to 1.8 mg of human cyclophilin a per ml of reaction ... | 2004 | 15479237 |
| distribution of escherichia coli o157 in bovine fecal pats and its impact on estimates of the prevalence of fecal shedding. | the distribution of escherichia coli o157 in bovine feces was examined by testing multiple samples from fecal pats and determining the density of e. coli o157 in immunomagnetic separation (ims)-positive fecal samples. the density of e. coli o157 in bovine feces was highly variable, differing by as much as 76,800 cfu g(-1) between samples from the same fecal pat. the density in most positive samples was <100 cfu g(-1), the limit of reliable detection by ims. testing only one 1-g sample of feces p ... | 2004 | 15466508 |
| detection and characterization of verocytotoxin-producing escherichia coli (vtec) o157 and non-o157 in cattle at slaughter. | between september 2001 to june 2002, 145 samples of bovine caecal content were collected at slaughter for verocytotoxin-producing escherichia coli (vtec) serogroups o157 and non-o157 detection. for e. coli o157 the immunomagnetic-separation technique was performed. the enterohaemolytic phenotype was the target for non-o157 vtec identification. the vero cell assay (vca) was performed for toxic activity detection. the genomic sequence for vt1, vt2 and intimin (vt1, vt2, eae genes) were identified ... | 2004 | 15460528 |
| ascophyllum nodosum supplementation: a preharvest intervention for reducing escherichia coli o157:h7 and salmonella spp. in feedlot steers. | ascophyllum nodosum (tasco-14) decreased the prevalence of enterohemorrhagic escherichia coli (ehec) o157:h7 in animals fed prior to harvest. tasco-14 was supplemented on a 2% dry matter basis 14 days prior to harvest to determine its effects on ehec and salmonella spp. prevalence. two hundred mixed crossbred steers and heifers (bos indicus x bos taurus), in a large commercial finishing facility, served as experimental units. treatment (trt, n = 100) animals received a steam-rolled corn-based di ... | 2004 | 15453570 |
| a nonradiometric, high-throughput assay for poly(adp-ribose) glycohydrolase (parg): application to inhibitor identification and evaluation. | the enzyme poly(adp-ribose) glycohydrolase (parg) catalyzes the hydrolysis of glycosidic bonds of adp-ribose polymers, producing monomeric adp-ribose units. thus, in conjunction with poly(adp-ribose) polymerase (parp), parg activity regulates the extent of in vivo poly(adp-ribosyl)ation. small molecule inhibitors of parp and parg have shown considerable promise in cellular models of ischemia-reperfusion injury and oxidative neuronal cell death. however, currently available parg inhibitors are no ... | 2004 | 15450800 |
| intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in babesia bovis. | members of the babesiarap-1 gene family are expressed during multiple parasite stages, and are regulated by both transcriptional and post-transcriptional mechanisms. in all babesia species, tandemly arranged rap-1 gene copies are separated by an intergenic (ig) region that is hypothesized to regulate gene expression. in this study, we tested that hypothesis by determining whether the babesia bovisrap-1 ig region could promote extra-chromosomal expression of exogenous genes introduced into merozo ... | 2004 | 15380689 |
| effect of pathogen-specific clinical mastitis on milk yield in dairy cows. | our objective was to estimate the effects of the first occurrence of pathogen-specific clinical mastitis (cm) on milk yield in 3071 dairy cows in 2 new york state farms. the pathogens studied were streptococcus spp.,staphylococcus aureus, staphylococcus spp., escherichia coli, klebsiella spp., arcanobacterium pyogenes, other pathogens grouped together, and "no pathogen isolated." data were collected from october 1999 to july 2001. milk samples were collected from cows showing signs of cm and wer ... | 2004 | 15377615 |
| dose dependency and individual variability of the lipopolysaccharide-induced bovine acute phase protein response. | in order to investigate the dose dependency and the individual variability of the lipopolysaccharide (lps)-induced acute phase protein response in cattle, 8 nonlactating, nonpregnant danish holstein cows were challenged 3 times each by intravenous injection of increasing doses (10, 100, and 1000 ng/kg, consecutively) of escherichia coli lps with 3-wk intervals. all 3 lps doses resulted in a rapid increase in serum concentrations of haptoglobin and serum amyloid a (saa) and a decrease in serum co ... | 2004 | 15377612 |
| water and ion permeation in baqp1 and glpf channels: a kinetic monte carlo study. | the kinetic monte carlo reaction-path-following technique is applied to determine the lowest-energy water pathway and the coordinating amino acids in baqp1 and glpf channels, both treated as rigid. in baqp1, water molecules pass through the pore between the asparagine-proline-alanine (npa) and selectivity filter (sf) sites one at a time. the water chain is interrupted at the sf where one water forms three stable hydrogen bonds with protein atoms. in this sf, water's conformation depends on the p ... | 2004 | 15377535 |
| a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. | to study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology. | 2004 | 15375107 |
| comparison of a membrane surface adhesion recovery method with an ims method for use in a polymerase chain reaction method to detect escherichia coli o157:h7 in minced beef. | in this study, enrichment procedures and two recovery methods, a membrane surface adhesion technique and an immunomagnetic separation (ims), were compared for use in conjunction with a multiplex polymerase chain reaction (pcr) method with a view to describing a fast (24 h) and economical test for detection of escherichia coli o157:h7 in meat samples. the study showed no significant difference between three different enrichment media (bhi, e. coli (e.c.) broth+novobiocin, modified tryptone soya b ... | 2004 | 15369860 |
| modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta. | plasminogen activator inhibitor-2 (pai-2) is an important counter proteolysis factor which helps protect tissues from inflammatory stress. the expression of pai-2 can be modulated by various inflammatory stimulants and mediators. the aim of the present study was to investigate how serum factors, might modulate the effects of lipopolysaccharide (lps) and interleukin-1beta on pai-2 production by human gingival fibroblasts. | 2004 | 15368874 |
| phage types and genotypes of shiga toxin-producing escherichia coli o157:h7 isolates from humans and animals in spain: identification and characterization of two predominating phage types (pt2 and pt8). | phage typing and dna macrorestriction fragment analysis by pulsed-field electrophoresis (pfge) were used for the epidemiological subtyping of a collection of shiga toxin-producing escherichia coli (stec) o157:h7 strains isolated in spain between 1980 and 1999. phage typing distinguished a total of 18 phage types among 171 strains isolated from different sources (67 humans, 82 bovines, 12 ovines, and 10 beef products). however, five phage types, phage type 2 (pt2; 42 strains), pt8 (33 strains), p ... | 2004 | 15364983 |
| the distribution of, and antibody response to, the core lipopolysaccharide region of escherichia coli isolated from the faeces of healthy humans and cattle. | there are five different core types of escherichia coli lipopolysaccharide (lps), and enterohaemorrhagic e. coli tend to have the r3 core type. it has been hypothesized that increased carriage of bacteria with a specific core type will induce higher levels of antibodies and protect against disease caused by bacteria carrying that specific lps core. approximately 320 isolates of e. coli, half from healthy human faeces and half from healthy bovine faeces have been core typed both by core-specific ... | 2004 | 15358817 |
| the incidence of shiga toxin-producing escherichia coli in cattle with mastitis in brazil. | to determine the prevalence and molecular characteristics of shiga toxin-producing escherichia coli (stec) isolates from bovine mastitic milk in brazil. | 2004 | 15357736 |
| fecal contamination of pastoral wetlands. | near-channel hill-country wetlands draining steep pastoral land in new zealand exhibit high levels of fecal contamination at a range of flows. this contamination is attributed to both the transport of bacteria into a wetland from the surrounding catchment and the direct excretion of fecal material onto wetlands by grazing cattle. e. coli concentrations observed at low to moderate flow at 20 sites varied between 0.5 x 10(1) and 2 x 10(4) most probable number (mpn) 100 ml(-1). high flow concentrat ... | 2004 | 15356253 |
| identification of intracellular target proteins of the calcium-signaling protein s100a12. | in this report, we have focused our attention on identifying intracellular mammalian proteins that bind s100a12 in a ca2+-dependent manner. using s100a12 affinity chromatography, we have identified cytosolic nadp+-dependent isocitrate dehydrogenase (idh), fructose-1,6-bisphosphate aldolase a (aldolase), glyceraldehyde-3-phosphate dehydrogenese (gapdh), annexin v, s100a9, and s100a12 itself as s100a12-binding proteins. immunoprecipitation experiments indicated the formation of stable complexes be ... | 2004 | 15355353 |
| changes of physicochemical indicators during mastitis and the effects of milk ejection on their sensitivity. | we examined the relationship between physicochemical indicators and somatic cells in the milk of dairy cows during experimentally induced mastitis and their significance as indicators for use in controlling udder health. we were concerned particularly with the effect of alveolar milk ejection on the sensitivity of these indicators. in expt 1, escherichia coli lipopolysaccharide (esch. coli lps) was injected into the left rear quarter to induce an inflammatory reaction in one quarter in each of s ... | 2004 | 15354578 |
| cryopreserved bovine mammary cells to model epithelial response to infection. | mammary gland epithelial cells are likely to be important effectors in defending against mastitis, yet little is known about their response mechanisms. here, we describe a cryopreserved bovine mammary epithelial cell model to study the infection response. primary cell cultures from four holstein cows were prepared, and frozen after two passages. the cell cultures from each cow were then thawed and maintained separately, yet simultaneously, and exposed to treatments that included infection with s ... | 2004 | 15350749 |
| high numbers of shiga toxin-producing escherichia coli found in bovine faeces collected at slaughter in japan. | the prevalence and concentration of shiga toxin-producing escherichia coli (stec) in cattle faeces (n=605) at the time of slaughter was studied in shimane prefecture, japan on a monthly basis between april 2000 and march 2001. screening with stx-pcr determined a prevalence of 37.5%. after analysis of spread faeces and enriched samples on cefixime, tellurite and sorbitol-macconkey agar using hcl treatment, 114 stec strains were singly or concomitantly isolated from 97 cattle (15.9%). of the 605 c ... | 2004 | 15336421 |
| influence of animal origin and lineage on survival of escherichia coli o157:h7 strains in strong and weak acid challenges. | twenty-five strains of escherichia coli o157:h7 isolated from humans, cattle, and pigs were maintained in hcl (ph 2.5) and in a volatile fatty acid (vfa) mixture (ph 4.0) for up to 6 h at 37 degrees c to assess their ability to survive in acidic conditions that simulate those of the stomach and ileum, respectively. in hcl, the average group survival of bovine strains was significantly higher than that of porcine and human strains, whereas in vfas, porcine strains were significantly more resistan ... | 2004 | 15330520 |
| iron uptake by escherichia coli cultured with antibodies from cows immunized with high-affinity ferric receptors. | the synergistic effects of immunoglobulin g (igg) from cows vaccinated with ferric citrate receptor (feca) and igg from cows vaccinated with ferric enterobactin receptor (fepa) were measured in an in vitro iron uptake assay. serum was isolated and pooled within treatment from five cows each vaccinated with fepa or feca or not vaccinated. immunoglobulin g was isolated by ammonium sulfate precipitation and protein g affinity chromatography. six escherichia coli isolates from bovine intramammary in ... | 2004 | 15328222 |
| antibiotic resistance among enterotoxigenic escherichia coli from piglets and calves with diarrhea. | in vitro resistance to 8 antimicrobials among enterotoxigenic escherichia coli from piglets and calves over a 13-year period was evaluated. least resistance occurred against ceftiofur for all, followed by apramycin and gentamicin for porcine and florfenicol for bovine isolates. no significant differences were found between the first 8 and last 5 years. | 2004 | 15317393 |
| effect of antibiotics in milk replacer on fecal shedding of escherichia coli o157:h7 in calves. | the objective of this study was to compare the concentration and duration of fecal shedding of escherichia coli o157:h7 between calves fed milk replacer with or without antibiotic (oxytetracycline and neomycin) supplementation. eighteen 1-wk-old holstein calves were orally inoculated with a strain of e. coli o157:h7 (3.6 x 10(8) cfu/calf) made resistant to nalidixic acid (na). rectal samples were obtained three times weekly for 8 wk following oral inoculation. fecal shedding of na-resistant e. c ... | 2004 | 15309963 |