Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| splenic macrophage activation and functions in amyloid enhancing factor-induced secondary amyloidosis. study of phagocytosis, killing, respiratory burst, and mhc class ii surface expression. | secondary amyloidosis is a systemic disease characterized by the extracellular tissue deposition of insoluble fibrillar amyloid a protein. aberrant metabolism of serum amyloid a protein by reticuloendothelial cells is thought to result in the accumulation of fibrils within the tissue. treatment of mice with amyloid-enhancing factor (aef) in conjunction with an inflammatory stimulus (i.e., agno3) induced amyloid deposition within 48-72 h. the activation state of a macrophage largely defines its e ... | 1993 | 8391054 |
| regulation of the prfa transcriptional activator of listeria monocytogenes: multiple promoter elements contribute to intracellular growth and cell-to-cell spread. | the prfa gene product is a transcriptional activator of listeria monocytogenes determinants of pathogenicity. in this study, we provide direct evidence that the prfa protein is a site-specific dna-binding protein. additionally, we describe the characterization of two classes of l. monocytogenes mutants which contain transposon insertions either in the prfa structural gene (exemplified by strain dp-l1075) or within the prfa promoter region (exemplified by strain dp-l973). both mutants are complet ... | 1993 | 8388865 |
| dual roles of plca in listeria monocytogenes pathogenesis. | the plca gene of listeria monocytogenes encodes a secreted phosphatidylinositol-specific phospholipase c (pl-plc). recent studies have established that transposon mutations within plca result in avirulence for mice and pleiotropic effects when examined in tissue-culture models of infection. genetic analysis reveals that many of the effects of the transposon insertions are due to loss of readthrough transcription from plca into the downstream gene prfa, which encodes an essential transcription fa ... | 1993 | 8388529 |
| the zinc metalloprotease of listeria monocytogenes is required for maturation of phosphatidylcholine phospholipase c: direct evidence obtained by gene complementation. | the maturation of the 33-kda proenzyme to the 29-kda phosphatidylcholine phospholipase c (pc-plc) of listeria monocytogenes requires the production of the zinc metalloprotease encoded by mpl, the proximal gene of the lecithinase operon. we recently described a low-virulence lecithinase-deficient mutant of l. monocytogenes egd-smr, designated jl762, generated by a single insertion of transposon tn1545 in mpl. this mutant failed to produce the 29-kda pc-plc, an exoenzyme probably involved in cell- ... | 1993 | 8384163 |
| mechanism of hepatic cytochrome p450 modulation during listeria monocytogenes infection in mice. | there have been numerous reports of altered drug clearance during episodes of viral infection and during the clinical use of recombinant interferons, but there have been very few reports regarding the effect of active bacterial infections on cytochrome p450-mediated metabolism. the objective of this study was to determine the mechanism by which the gram-positive bacteria listeria monocytogenes causes a depression of cytochrome p450-mediated biotransformation in mice. after induction with beta-na ... | 1993 | 8379989 |
| listeria meningitis: identification of a cerebrospinal fluid inhibitor of macrophage listericidal function as interleukin 10. | the killing of bacteria gaining access to the central nervous system is insufficient and requires bactericidal antibiotics for treatment. the inefficient host response in cerebrospinal fluid (csf) is thought to be due to impaired phagocytosis in csf, and low local concentration of antibody and complement. in addition, the csf may contain inhibitors, disabling phagocytes to eliminate bacteria. we have assessed the bactericidal activity of macrophages in the presence of csf from mice infected intr ... | 1993 | 8376933 |
| macrophage colony-stimulating factor and the enhanced migration of monocytes are essential in primary but not secondary host defenses to listeria organisms. | nonimmune mice infected with listeria monocytogenes exhibited elevated expression of macrophage colony-stimulating factor (m-csf) mrna and the enhanced migration of mac-1 antigen-positive bone marrow-derived mononuclear phagocytes (bmmp) to their livers. treatment with monoclonal anti-m-csf antibody diminished the traffic of bmmp and promoted the replication of listeriae. immune animals infected with listeriae expressed significantly lower levels of m-csf mrna than did nonimmune animals. moreove ... | 1993 | 8376839 |
| growth of listeria monocytogenes in camembert and other soft cheeses at refrigeration temperatures. | listeria monocytogenes survived and, under most conditions, multiplied when inoculated directly into the cheese milk of laboratory made camembert cheeses. the rate and extent of growth was reduced at lower storage temperatures. significantly higher rates of growth occurred at the surface compared with the centre of the cheeses, and these were probably associated with increased ph and proteolysis at the cheese surface due to the mould ripening process. similar results were obtained with camenbert ... | 1993 | 8376636 |
| polysaccharides isolated from plant cell cultures of echinacea purpurea enhance the resistance of immunosuppressed mice against systemic infections with candida albicans and listeria monocytogenes. | polysaccharides (ep) isolated from large scale plant cell cultures of echinacea purpurea, have been shown to activate human and murine phagocytes. in this study we investigated the influence of ep on the nonspecific immunity in immunodeficient mice. ep was effective in activating peritoneal macrophages isolated from animals after administration of cyclophosphamide (cp) or cyclosporin a (csa). ep-treated macrophages exhibited increased production of tumor necrosis factor-alpha (tnf) and enhanced ... | 1993 | 8375943 |
| effect of lps on epithelial integrity and bacterial uptake in the polarized human enterocyte-like cell line caco-2. | bacterial lipopolysaccharide (lps) has been speculated to facilitate bacterial translocation by a mechanism involving physical disruption of the gut mucosal barrier. polarized, cultured intestinal epithelial cells (caco-2 cells) were used to study the effect of lps on enterocyte structure, viability, and susceptibility to bacterial invasion. varying concentrations of biologically active lps were incubated with enterocytes for 1 and 16 hr. lps had no noticeable effect on enterocyte viability or m ... | 1993 | 8375030 |
| growth response of listeria monocytogenes nctc 7973 in two different media at four incubation temperatures. | listeria monocytogenes nctc 7973 was cultivated in tryptose phosphate broth (tpb) and tryptone soya broth (tsb) at four different temperatures: 4 degrees, 20 degrees, 30 degrees and 37 degrees c. cells grown in tsb at 20 degrees c displayed maximum absorbance over cells grown in tpb at the same temperature. moreover, this observation was further confirmed by dry mass determination and viable count. we determined that cells grown in tsb at 20 degrees c exhibited significant enhanced growth as com ... | 1993 | 8373108 |
| listeriosis of the placenta: clinicopathologic study of seven cases. | the purpose of our study was to evaluate the usefulness of placental examination in the diagnosis of prenatal listeriosis. | 1993 | 8372871 |
| characterization of a new class of tetracycline-resistance gene tet(s) in listeria monocytogenes bm4210. | the nucleotide sequence of the tetracycline (tc)-minocycline (mc)-resistance determinant of plasmid pip811 from listeria monocytogenes bm4210 has been determined. the gene, designated tet(s), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated m(r) of 72,912. the apparent 68-kda size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcript ... | 1993 | 8370538 |
| cellular motions and thermal fluctuations: the brownian ratchet. | we present here a model for how chemical reactions generate protrusive forces by rectifying brownian motion. this sort of energy transduction drives a number of intracellular processes, including filopodial protrusion, propulsion of the bacterium listeria, and protein translocation. | 1993 | 8369439 |
| detection of listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay. | a polymerase chain reaction (pcr)-coupled ligase chain reaction (lcr) assay for the specific detection of listeria monocytogenes (m. wiedmann, j. czajka, f. barany, and c. a. batt, appl. environ. microbiol. 58:3443-3447, 1992) has been modified for detection of the lcr products with a nonisotopic readout. when a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the lcr products was used, the pcr-coupled lcr gave a sensitivity of 10 cfu of l. monocytogenes. the detecti ... | 1993 | 8368859 |
| monoclonal antibodies that react with live listeria spp. | seven monoclonal antibodies (mabs) against listeria spp. that were reactive with live listeria spp. were developed. two of these mabs (55-8 and 55-37) were members of the immunoglobulin m class, and all other mabs were members of the immunoglobulin g class. mab 55-23 reacted with 148 of 157 strains tested. mab 34-51 reacted with serotype 1/2a, 1/2b, and 1/2c strains and exhibited a scattered reaction pattern with strains belonging to other serotypes. mab 55-44 reacted with all of the strains bel ... | 1993 | 8368856 |
| a ribosomal dna fragment of listeria monocytogenes and its use as a genus-specific probe in an aqueous-phase hybridization assay. | cdnas were prepared from the total rna of listeria monocytogenes atcc 19118 and used as probes to screen a genomic library of the same strain. four clones were identified which contained ribosomal dna fragments. recombinant dna from one of them was fractionated and differentially hybridized with the cdna probes to rna of l. monocytogenes and kurthia zopfii. the resulting hybridization pattern revealed an hpaii fragment of 0.8 kb that was specific for the l. monocytogenes strain. the nucleotide s ... | 1993 | 8368854 |
| inhibition of listeria monocytogenes by lactobacillus bavaricus mn in beef systems at refrigeration temperatures. | the ability of lactobacillus bavaricus, a meat isolate, to inhibit the growth of three listeria monocytogenes strains was examined in three beef systems: beef cubes, beef cubes in gravy, and beef cubes in gravy containing glucose. the beef was minimally heat treated, inoculated with l. bavaricus at 10(5) or 10(3) cfu/g and l. monocytogenes at 10(2) cfu/g, vacuum sealed, and stored at 4 or 10 degrees c. the meat samples were monitored for microbial growth, ph, and bacteriocin production. the path ... | 1993 | 8368843 |
| a combined modified reverse dot-blot and nested pcr assay for the specific non-radioactive detection of listeria monocytogenes. | a modified reverse dot-blot assay was developed and used in combination with a nested pcr amplification of hly a for the specific detection of listeria monocytogenes. the deoxynucleotides and digoxygenin-11-dutp concentrations in the pcr were optimized for maximal sensitivity and economy. for the dot-blot hybridization, digoxygenin-labelled pcr products were directly spotted on nylon membrane-bound poly-dt tailed capture probes and the signal detection was either colorimetric or chemiluminescent ... | 1993 | 8366865 |
| inhibition of enterobacteria and listeria growth by lactic, acetic and formic acids. | minimum inhibitory concentrations (mic) of undissociated lactic, acetic and formic acids were evaluated for 23 strains of enterobacteria and two of listeria monocytogenes. the evaluation was performed aerobically and anaerobically in a liquid test system at ph intervals of between 4.2 and 5.4. growth of the enterobacteria was inhibited at 2-11 mmol l-1, 0.5-14 mmol l-1 and 0.1-1.5 mmol l-1 of undissociated lactic, acetic and formic acids, respectively. the mic value was slightly lower with anaer ... | 1993 | 8365950 |
| an animal model of foodborne listeria monocytogenes virulence: effect of alterations in local and systemic immunity on invasive infection. | development of foodborne listeriosis is dependent on an interplay between organism-specific virulence factors and host susceptibility. gastric inoculation of sprague-dawley rats was used as a model to explore listeria-specific virulence and host susceptibility. gastric inoculation leads to invasive infection with "smooth" hemolytic listeria monocytogenes but not with "rough" l. monocytogenes or other listeria species. infection is dose-dependent with an id50 of 10(6) virulent listeria monocytoge ... | 1993 | 8365049 |
| different roles of alpha beta and gamma delta t cells in immunity against an intracellular bacterial pathogen. | several bacterial pathogens of medical importance are able to persist and replicate inside host mononuclear phagocytes. protective immunity depends on specific t lymphocytes that induce granulomatous lesions at the sites of bacterial multiplication. listeria monocytogenes is an intracellular pathogen that replicates inside mononuclear phagocytes and hepatocytes of mice. invasion from the phagosomal compartment into the cytoplasmic compartment is the principal mechanism of intracellular survival. ... | 1993 | 8361537 |
| intracytoplasmic growth and virulence of listeria monocytogenes auxotrophic mutants. | the intracellular growth of several auxotrophic mutants of listeria monocytogenes was examined in cell culture, and virulence was evaluated in mice by intravenous injection of log-phase bacteria. l. monocytogenes transposon insertion mutants requiring either uracil, phenylalanine, glycine, proline, or nicotinic acid for growth were fully virulent and grew similarly to the parental strain as shown by their growth rates in cell culture. those requiring all three aromatic amino acids (phenylalanine ... | 1993 | 8359896 |
| analysis of cytokine mrna expression in listeria-resistant c57bl/6 and listeria-susceptible a/j mice during listeria monocytogenes infection. | this laboratory previously reported that mrna expression for many cytokines, as determined by reverse transcription-polymerase chain reaction analysis, is induced rapidly in the spleen during murine listeriosis. in the present study, the patterns of cytokine mrna expression in spleens and livers of listeria-resistant c57bl/6 and listeria-susceptible a/j mice were compared. in addition, in situ hybridization was performed to evaluate the distributions of cytokine mrna-expressing cells in these ti ... | 1993 | 8359895 |
| role of complement component c1q in phagocytosis of listeria monocytogenes by murine macrophage-like cell lines. | listeria monocytogenes is a facultative intracellular pathogen of a great variety of cells. among them, macrophages constitute the major effector cells of listerial immunity during the course of an infection. although the molecular bases of l. monocytogenes attachment and entry to phagocytes are not completely understood, it has been demonstrated that c3b significantly increases l. monocytogenes uptake by macrophages via complement receptor type 3. the first component of complement, c1q, is pres ... | 1993 | 8359889 |
| inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish. | the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ... | 1993 | 8357253 |
| sodium chloride, potassium chloride, and virulence in listeria monocytogenes. | virulence, as determined in a mouse model, and the virulence factor activities of catalase, superoxide dismutase, and listeriolysin o were examined in a parental strain (10403s) and in a nonhemolytic mutant strain (dp-l224) of listeria monocytogenes. the cells were propagated in media containing various concentrations of sodium chloride or potassium chloride. strains 10403s and dp-l224 exhibited significant increases in catalase activity and listeriolysin o activity when grown in medium containi ... | 1993 | 8357245 |
| inhibition of adhesion of enteroinvasive pathogens to human intestinal caco-2 cells by lactobacillus acidophilus strain lb decreases bacterial invasion. | salmonella typhimurium and enteropathogenic escherichia coli (epec) were found to adhere to the brush border of differentiated human intestinal epithelial caco-2 cells in culture, whereas yersinia pseudotuberculosis and listeria monocytogenes adhered to the periphery of undifferentiated caco-2 cells. all these enterovirulent strains invaded the caco-2 cells. using a heat-killed human lactobacillus acidophilus (strain lb) which strongly adheres both to undifferentiated and differentiated caco-2 c ... | 1993 | 8354463 |
| ovine ophthalmitis associated with listeria monocytogenes. | 1993 | 8351784 | |
| [neurotropic properties of listeria monocytogenes and mycobacterium leprae in natural and experimental infections]. | 1993 | 8351384 | |
| induction of murine peritoneal gamma/delta t cells and their role in resistance to bacterial infection. | previous studies have reported an association of gamma/delta t cells with microbial infection in both human lesions and murine infectious disease models. in this study we provide a comprehensive analysis of the conditions under which the induction of gamma/delta t cells occurs at a site of infection. we found a site-specific induction of gamma/delta t cells after the injection of listeria monocytogenes in the peritoneal cavity of c3h mice. no changes were seen in the splenic or lymph node popula ... | 1993 | 8350063 |
| listeria monocytogenes--a model system for studying the pathomechanisms of an intracellular microorganism. | virulence of listeria monocytogenes is determined by a cluster of five genes in the order plca, hly, mpl, acta and plcb, which are coordinately regulated by a transcriptional activator, termed prfa. the gene for prfa is located in front of plca. mutations within each of these genes reduce the virulence considerably and render the mutants unable to properly multiply and/or spread within the infected host cells. under growth-limiting conditions prfa-dependent proteins are preferentially synthesise ... | 1993 | 8347937 |
| presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica in environmental samples taken from a supermarket delicatessen. | environmental swab samples collected on three occasions from a supermarket delicatessen were examined for the presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica. strains of y. enterocolitica belonging to a pathogenic biotype were not recovered, but isolates of l. monocytogenes and motile aeromonads were found. contaminated surfaces and items that came into direct contact with ready-to-eat food included a slicing machine, a knife and parsley that was to be used as a ... | 1993 | 8347431 |
| distribution of listeria spp. in confectioners' pastries from western france: comparison of enrichment methods. | three hundred samples of pastry from 100 different suppliers in western france, including butter-cream, whipped dairy cream and custard filled cakes from each supplier, were collected and tested for the occurrence of listeria spp. in 25 g samples. listeria spp. were detected in 21.7% of he samples: listeria monocytogenes in 13.7%, listeria innocua in 10% and listeria seeligeri in 2.3%. thirteen samples were contaminated with two species simultaneously. the frequency of contaminated samples was n ... | 1993 | 8347428 |
| the toxicologic interactions resulting from inhalation of carbon black and acrolein on pulmonary antibacterial and antiviral defenses. | the goal of this study was to investigate whether coexposures to carbon black and acrolein result in a toxicologic interaction having effects on lung defenses against infectious agents. this aim was accomplished through in vivo studies with inhalation challenges of infectious agents that probe the functional integrity of the multicomponent system that comprises the integrated defenses of the lungs. staphylococcus aureus was used for the alveolar macrophage (am) surveillance phagocytic system, pr ... | 1993 | 8346533 |
| variable binding affinities of listeriolysin o peptides for the h-2kd class i molecule. | previously we used the peptide-binding motif for the murine class i major histocompatibility complex molecule h-2kd to identify a nonamer peptide of the listeria monocytogenes listeriolysin (llo) protein that was recognized by cytotoxic t lymphocytes (ctl) in association with h-2kd. eleven nonamer peptides contained in the llo sequence were synthesized and one, llo 91-99, proved to be a ctl target. using peptide binding competition assays with h-2kd-restricted ctl, we show that 3 out of the 11 l ... | 1993 | 8344365 |
| [weber syndrome caused by listeria abscess]. | a 63-year old diabetic man presented with left weber's syndrome and meningitic syndrome. csf examination showed moderate lymphocytic pleocytosis and elevated proteins with normal glucose content and sterile culture. blood cultures yielded listeria monocytogenes and the patient received ampicillin. while his neurological condition had partially improved, he died of heart failure. several mesencephalic abscesses were found at autopsy. | 1993 | 8337566 |
| bacterial phospholipases c. | a variety of pathogenic bacteria produce phospholipases c, and since the discovery in 1944 that a bacterial toxin (clostridium perfringens alpha-toxin) possessed an enzymatic activity, there has been considerable interest in this class of proteins. initial speculation that all phospholipases c would have lethal properties has not been substantiated. most of the characterized enzymes fall into one of four groups of structurally related proteins: the zinc-metallophospholipases c, the sphingomyelin ... | 1993 | 8336671 |
| ifn-gamma inhibits the replication of listeria monocytogenes in hepatocytes. | we previously reported that the bulk of listeria monocytogenes injected intravenously into mice is taken up in the liver and replicates within the parenchymal cells (hepatocytes). although ifn-gamma is known to play an important role in host defenses to listerial infections of the liver, the mechanism(s) that underlies this role remains to be fully delineated. in the initial experiments presented here, we demonstrated the elevated expression of ifn-gamma message in the livers of mice during prim ... | 1993 | 8335936 |
| immunization of mice with yersinia enterocolitica leads to the induction of antithyrotropin receptor antibodies. | it is well established that autoimmune graves' disease, which is characterized by hyperthyroidism, is mediated by autoantibodies to the thyrotropin receptor (tshr). although what initially triggers this autoantibody response is not known, a number of studies have suggested that yersinia enterocolitica, an enterobacteria, could initiate the immune response against the tshr. in this study, we produced antibodies against purified extracellular domain of human tshr (etshr) and showed that anti-etshr ... | 1993 | 8335920 |
| protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins. | porcine leukocytes contained three homologous peptides, pg-1, 2 and 3, that manifested potent microbicidal activity against escherichia coli, listeria monocytogenes and candida albicans in vitro. the peptides ('protegrins') were composed of 16 (pg-2) or 18 amino acid residues, and, like tachyplesins (broad-spectrum antibiotic peptides of horseshoe crab hemocytes), they contained two intramolecular cystine disulfide bonds. considerably smaller than defensins, protegrins nevertheless showed substa ... | 1993 | 8335113 |
| purification and characterization of a 58-kda cell wall-associated protein from listeria monocytogenes. | a cell wall protein (p58) was purified from listeria monocytogenes by detergent extraction and superose 6 gel chromatography. it had a molecular mass of 58 kda, was strongly hydrophobic, contained reactive thiol group(s) and was located at least partially on the surface of bacterial cells. production of this protein varied among different listeria, being the most prominent in nctc 7973 of l. monocytogenes, weaker in four other strains of this species and undetectable in tested strains of l. seel ... | 1993 | 8332104 |
| expression of listeriolysin and phosphatidylinositol-specific phospholipase c is repressed by the plant-derived molecule cellobiose in listeria monocytogenes. | the primary habitat of the intracellular pathogen listeria monocytogenes is considered to be soil and decaying vegetation. as an opportunistic pathogen it must be able to recognize its entry into host tissue and, in response, co-ordinately induce the expression of virulence factors. no signature molecule, which facilitates this regulation, has been identified for any human pathogen. our studies have demonstrated for the first time that the expression of major virulence determinants in l. monocyt ... | 1993 | 8332058 |
| nonspecific phospholipase c of listeria monocytogenes: activity on phospholipids in triton x-100-mixed micelles and in biological membranes. | listeria monocytogenes secretes a phospholipase c (plc) which has 39% amino acid sequence identity with the broad-specificity plc from bacillus cereus. recent work indicates that the l. monocytogenes enzyme plays a role during infections of mammalian cells (j.-a. vazquez-boland, c. kocks, s. dramsi, h. ohayon, c. geoffroy, j. mengaud, and p. cossart, infect. immun. 60:219-230, 1992). the homogeneous enzyme has a specific activity of 230 mumol/min/mg when phosphatidylcholine (pc) is dispersed in ... | 1993 | 8331063 |
| reduction of ferric iron by listeria monocytogenes and other species of listeria. | one mechanism by which listeria monocytogenes is thought to obtain iron required for growth is through the extracellular reduction of a ferric iron source to the ferrous form. to better characterize this reductase activity we have developed a simple plate assay that allows detection of colonies of listeria species able to reduce ferric iron. cells are plated on an agar base medium containing a ferric iron source and ethylenediamine dihydroxyphenylacetic acid. colonies are then overlain with soft ... | 1993 | 8330259 |
| comparison of "gen-probe" dna probe and pcr for detection of listeria monocytogenes in naturally contaminated soft cheese and semi-soft cheese. | a comparison was made of three approaches for the detection of listeria monocytogenes in naturally contaminated soft cheese and semi-soft cheese after enrichment. enrichment broths were tested by plating them onto different selective agars, by "gen probe" dna hybridization and by the polymerase chain reaction (pcr). based on two-step enrichment, all three approaches showed high specificities (90% or more) in detecting l. monocytogenes. in contrast, the sensitivity of the gen-probe test was low ( ... | 1993 | 8327782 |
| inhibition of psychrotrophic organisms by propionicin plg-1, a bacteriocin produced by propionibacterium thoenii. | propionibacterium thoenii strain p127, which produces the bacteriocin propionicin plg-1, was grown in a skim milk medium and produced bacteriocin in that medium. no bacteriocin activity was detected in skim milk medium in which strain p127-1, a bacteriocin-negative variant of strain p127, had been grown. five psychrotrophic spoilage or pathogenic organisms (one strain each of listeria monocytogenes, pseudomonas fluorescens, vibrio parahaemolyticus, yersinia enterocolitica, and one strain of cory ... | 1993 | 8326023 |
| enhanced conjugative transfer of plasmid dna from escherichia coli to staphylococcus aureus and listeria monocytogenes. | transfer of mobilizable shuttle cloning vectors by conjugation from escherichia coli to staphylococcus aureus occurred at a very low frequency (10(-9) transconjugants per donor colony-forming unit after the mating period). it was observed that subinhibitory concentrations of penicillins (oxacillin or penicillin g) in the mating medium resulted in increased transfer frequency by conjugation of the shuttle vector pat18 from e. coli sm10 to s. aureus 80cr5 str (54-fold) and to listeria monocytogene ... | 1993 | 8319881 |
| rapid determination of listeria monocytogenes in foods using a resuscitation/selection/kit system detection. | a resuscitation medium consisting of a trypticase soy broth base supplemented with 0.5% yeast extract, 0.25% sodium pyruvate, 0.01% sodium thioglycollate, and 0.1% chicken fat was used in the resuscitation of heat-injured and freeze-injured cells of listeria monocytogenes. after a resuscitation period of 4-h, the medium was made selective through the addition of nalidixic acid, acriflavin, and cycloheximide. the organisms were incubated in the selectivized medium at 35 degrees c for an additiona ... | 1993 | 8318857 |
| surface-associated, prfa-regulated proteins of listeria monocytogenes synthesized under stress conditions. | the expression of the five clustered genes of listeria monocytogenes: plca, hly, mpl, acta and plcb is under the control of the positive regulation factor prfa. listeriolysin, encoded by the hly gene, is the only prominent prfa-controlled gene product observed when l. monocytogenes strain nctc 7973 is cultured in a rich medium at 37 degrees c to the logarithmic growth phase. stress conditions such as heat-shock or stationary culture conditions lead to the induction of additional prfa-dependent p ... | 1993 | 8316076 |
| kinetics of antibody production against listeriolysin o in sheep with listeriosis. | the kinetics of antibody production against listeriolysin o (llo), a major virulence factor of the intracellular bacterial pathogen listeria monocytogenes, was studied by dot blot analysis with highly purified llo during oral infection of sheep. specific antibodies appeared as soon as day 9 of an oral infection and peaked by day 20 of infection; specific antibody levels then remained almost stable for at least 4 months. a subclinical infecting dose (approximately 10(6) viable bacteria) was capab ... | 1993 | 8314995 |
| involvement of profilin in the actin-based motility of l. monocytogenes in cells and in cell-free extracts. | within hours of listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. we have devised a cell-free extract system capable of faithfully reconstituting l. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. we find that extracts fr ... | 1994 | 8313471 |
| behaviour of listeria monocytogenes during the maturation of naturally and artificially contaminated salami: effect of lactic-acid bacteria starter cultures. | the antimicrobial activity of a bacteriocin-producing lactobacillus plantarum mcs strain against listeria monocytogenes in naturally and artificially contaminated salami was evaluated in comparison with that of other non bacteriocin-producing lactic-acid bacteria (i.e., l. plantarum mcs1 mutant strain and two commercial starter cultures). in salami not inoculated with lactic-acid bacteria, moderate l. monocytogenes growth was observed after 7-14 days. the addition of the starter cultures prevent ... | 1993 | 8312141 |
| listeria monocytogenes udder infection and carcase contamination. | 1993 | 8310624 | |
| cell interactions of listeria monocytogenes l forms and peritoneal exudative cells in rats. | listeria monocytogenes 4b and its forms without cell walls (l forms of a protoplastic type) were used to study in vivo interactions with host cells. samples of peritoneal lavage fluid were obtained from rats intraperitoneally inoculated at intervals between 1 and 15 days after challenge, for scanning electron microscopic, bacteriological, biochemical, and cytometrical investigations. scanning electron microscopic examination revealed continuous adhesion of l forms on the macrophage surface up to ... | 1993 | 8306205 |
| diffuse petechial pustular lesions in a newborn. disseminated listeria monocytogenes. | 1994 | 8304768 | |
| [neonatal listeriosis. a case report]. | the author describes a case of early neonatal listeriosis following by a positive outcome. the severity of this infection is underlined as well as the need to examine carefully the role of listeria monocytogenes as a cause of similar influenza-type syndromes in pregnant women. | 1993 | 8302227 |
| bacterium-host cell interactions at the cellular level: fluorescent labeling of bacteria and analysis of short-term bacterium-phagocyte interaction by flow cytometry. | flow cytometry is a potentially powerful tool for analyzing the interactions of facultative intracellular bacteria and macrophages on a cellular level, particularly when fluorochromes are used to label the bacteria. we labeled listeria monocytogenes and salmonella typhimurium with a lipophilic dye, pkh-2, and used flow cytometry to investigate phagocytosis by j774a.1 cells and short-term bacterial survival. labeled and unlabeled bacteria were identical in terms of viability, growth kinetics, and ... | 1994 | 8300223 |
| fate of listeria monocytogenes in murine macrophages: evidence for simultaneous killing and survival of intracellular bacteria. | the intracellular survival of the ubiquitous pathogen listeria monocytogenes was studied in primary cultures of bone marrow-derived mouse macrophages. bacteria were able to grow rapidly in these cells, with an apparent multiplication rate of about 40 min. electron microscopy demonstrated that intracellular bacterial replication was the consequence of simultaneous intracellular killing and replication of bacteria in the same cells. within the first hour following phagocytosis, most bacteria were ... | 1994 | 8300212 |
| induction of cytokines in phagocytic mammalian cells infected with virulent and avirulent listeria strains. | the present paper analyzes the cytokine response of mouse macrophages during infection by listeria monocytogenes. the use of different mutants of l. monocytogenes impaired in various steps of the infection process allowed us to dissect the cytokine response. cytokine mrna expression was detected by pcr-assisted amplification of rna extracted from macrophages after infection with different listeria strains. an increase in the amount of mrna for tumor necrosis factor alpha (tnf-alpha), interleukin ... | 1994 | 8300196 |
| listeria meningitis after bone marrow transplantation. | over the past decade infections from food-borne listeria monocytogenes have become an important cause of septicaemia and meningitis and immunocompromised patients are at particular risk. we report three cases of listeria meningitis occurring post-bmt. the patients were aged 53, 51 and 56 years and presented 4, 7 and 90 months post-transplant, respectively. the first patient had undergone allogeneic bmt for myelodysplasia and the other two patients had abmt for aml in second and first cr, respect ... | 1993 | 8298566 |
| effects of buspirone on the resistance, development and passive transfer of immunity to listeria monocytogenes in mice submitted to stress. | mice exposed to a chronic auditory stressor and treated chronically with buspirone (1 mg/kg) showed a reduction in stress-induced suppression of the resistance and development of immunity to listeria monocytogenes. attempts to passively transfer immunity with spleen cells were also performed. stressed, immunized mice had a reduced capacity to transfer immunity passively to nonimmunized mice and buspirone was found to partially suppress this inhibitory effect of stress. | 1993 | 8297183 |
| the haemophilus influenzae dnag sequence and conserved bacterial primase motifs. | the dnag gene encodes primase which synthesizes the primer rna essential for escherichia coli chromosomal dna replication. the nucleotide sequence was determined for the haemophilus influenzae dnag gene and used in the molecular evolutionary analysis of primases from six bacterial species. the predicted amino acid (aa) sequence of h. influenzae dnag contains 593 residues and shares 56% identity with e. coli dnag. the n-terminal 60% of six aligned bacterial primases contains all 71 absolutely con ... | 1993 | 8294018 |
| glycine betaine confers enhanced osmotolerance and cryotolerance on listeria monocytogenes. | listeria monocytogenes is a gram-positive food-borne pathogen that is notably resistant to osmotic stress and can grow at refrigerator temperatures. these two characteristics make it an insidious threat to public health. like several other organisms, l. monocytogenes accumulates glycine betaine, a ubiquitous and effective osmolyte, intracellularly when grown under osmotic stress. however, it also accumulates glycine betaine when grown under chill stress at refrigerator temperatures. exogenously ... | 1994 | 8288538 |
| isolation and characterization of listeria monocytogenes-specific nucleotide sequences. | subtracter probe hybridization was used to screen a partial genomic library of a clinical isolate of listeria monocytogenes. three clones that hybridized with genomic dna from 174 strains of l. monocytogenes but not with genomic dna from 32 strains representing other listeria spp. were recovered. these data establish the utility of subtracter probe hybridization for recovering l. monocytogenes-specific sequences. | 1993 | 8285722 |
| prolonged and effective blockade of tumor necrosis factor activity through adenovirus-mediated gene transfer. | a chimeric protein capable of binding and neutralizing tumor necrosis factor (tnf) and lymphotoxin was expressed in mice transduced with a replication-incompetent adenoviral vector into which a tnf inhibitor gene had been engineered. within 3 days following the injection of 10(9) infectious particles, the tnf inhibitor concentration exceeded 1 mg/ml of plasma; this level of expression was maintained for at least 4 weeks, and detectable tnf inhibitory activity was measured 6 weeks after injection ... | 1994 | 8278368 |
| neutrophils are essential for early anti-listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody. | this study shows that in mice selectively depleted of neutrophils by treatment with a monoclonal antibody, rb6-8c5, listeriosis is severely exacerbated in the liver, but not in the spleen or peritoneal cavity during the crucial first day of infection. at sites of infection in the livers of neutrophil-depleted mice, listeria monocytogenes grew to large numbers inside hepatocytes. by contrast, in the livers of normal mice neutrophils rapidly accumulated at infectious foci and this was associated w ... | 1994 | 8270870 |
| heat resistance of listeria monocytogenes in vacuum packaged pasteurized fish fillets. | the heat resistance of two strains of listeria monocytogenes in sous-vide cooked fillets of cod and salmon was investigated. fish sticks of 5 g were inoculated, vacuum-packed and heated at different combinations of time and temperature (58-80 degrees c). time-temperature combinations allowing survival and time-temperature combinations at which the bacteria were destroyed, were used to determine d- and z-values. d-values were in the range of what has been published for other food products. d60-va ... | 1993 | 8268058 |
| microbiological quality of artisanal ice cream. | in the course of the years 1990 and 1991, 396 samples of artisanal ice-cream had been collected from different ice-cream shops in udine and province and analysed. all tested flavours contained aerobic germs, coliforms, enterococci and yeasts in different quantities. in the analysed samples neither salmonellae spp., nor listeria monocytogenes nor staphylococcus aureus were detected. numerous species of coliforms and yeasts were randomly isolated and identified. the statistical analysis, used to c ... | 1993 | 8267839 |
| isolation of listeria monocytogenes from goat cheese associated with a case of listeriosis in goat. | listeria monocytogenes was isolated from the brain of a goat, which was euthanized due to listeriosis. a few weeks later a similar subtype of l. monocytogenes was isolated from an on-farm manufactured fresh cheese which did not contain any milk from the goat which had suffered from listeriosis. a similar subtype was also found on 1 of the shelves in the refrigerator where cheeses were stored. prior to the onset of listeriosis, 1 fresh cheese had been made of milk from the actual goat, which may ... | 1993 | 8266892 |
| expression and phosphorylation of the listeria monocytogenes acta protein in mammalian cells. | movement of listeria monocytogenes within infected eukaryotic cells provides a simple model system to study the mechanism of actin-based motility in nonmuscle cells. the acta gene of l. monocytogenes is required to induce the polymerization of host actin filaments [kocks, c., gouin, e., tabouret, m., berche, p., ohayon, h. & cossart, p. (1990) cell 68, 521-531; domann, e., wehland, j., rohde, m., pistor, s., hartl, m., goebel, w., leimeister-wachter, m., wuenscher, m. & chakraborty, t. (1992) em ... | 1993 | 8265643 |
| the effects of diacetate with nitrite, lactate, or pediocin on the viability of listeria monocytogenes in turkey slurries. | the antilisterial effects of sodium diacetate (0.1, 0.3 and 0.5%) alone or in combination with sodium nitrite (30 ppm), sodium lactate (2.5%) or pediocin (5000 arbitrary units/ml) were evaluated in slurries (25% meat in sterile deionized h2o) prepared from vacuum-packaged, ready-to-eat turkey breast meat and challenged with listeria monocytogenes. in the absence of food additives, counts of l. monocytogenes increased from 4.5 log10 cfu/ml to ca. 8 log10 cfu/ml within 1 day at 25 degrees c and wi ... | 1993 | 8257656 |
| detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products. | forty bacteriocin-producing (bac+) lactic acid bacteria (lab) were isolated from food samples purchased from retail supermarkets and local farms. of the 40 bac+ isolates, 18 were isolated from 85 food samples by enrichment (21% isolation rate) whereas eight were obtained from 63 samples by direct plating (13% isolation rate). by direct plating, bac+ lab were detected at levels up to 2.4 x 10(5) cfu/g in ready-to-eat meats. the bac+ isolates were identified by carbohydrate fermentation patterns, ... | 1993 | 8257654 |
| listeria monocytogenes phosphatidylinositol (pi)-specific phospholipase c has low activity on glycosyl-pi-anchored proteins. | the ability of the phosphatidylinositol-specific phospholipase c (pi-plc) from listeria monocytogenes to hydrolyze glycosyl phosphatidylinositol (gpi)-anchored membrane proteins was compared with the ability of the pi-plc from bacillus thuringiensis to hydrolyze such proteins. the l. monocytogenes enzyme produced no detectable release of acetylcholinesterase from bovine, sheep, and human erythrocytes. the cleavage of the gpi anchors of alkaline phosphatase from rat and rabbit kidney slices was l ... | 1993 | 8253689 |
| macrophage-listeria interactions. | it is clear that the interaction between macrophages and the facultative intracellular bacterium listeria monocytogenes is complex and dictated by the needs of both organisms. on the one hand, the invading pathogen has devised strategies to locate itself intracellularly in a site where host defense mechanisms are minimal; thus it escapes the phagosome and enters the cytoplasm, from which it travels to the next cell without exposing itself to the extracellular environment. on the other hand, the ... | 1994 | 8251578 |
| growth of listeria monocytogenes, aeromonas hydrophila and yersinia enterocolitica in pâté and a comparison with predictive models. | a reference or type strain and a food derived-strain of the cold-tolerant pathogens listeria monocytogenes, aeromonas hydrophila and yersinia enterocolitica were individually inoculated into samples of commercial pâté and incubated at 4 and 10 degrees c. the organisms were periodically estimated by presumptive counts, then values for the lag and generation times were calculated. both strains of l. monocytogenes grew at both temperatures. the food strain of a. hydrophila grew only at 10 degrees c ... | 1993 | 8251302 |
| effects of glucose, growth temperature, and ph on listeriolysin o production in listeria monocytogenes. | expression of listeriolysin o of listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. expression of listeriolysin o was reduced at a lower growth temperatures (26 degrees c) and at higher glucose concentrations (> or = 0.3%) in the growth media. the effect of glucose appeared to be due to a change in the ph of the growth media. | 1993 | 8250571 |
| [neuromeningeal listeriosis in adults. clinical aspects and contribution of cotrimoxazole in monotherapy]. | a series of 28 patients suffering from neuromeningeal listeriosis is reported. this disease is consecutive to infection by listeria monocytogenes--an ubiquitous and opportunistic gram-positive bacillus--and has become a public health problem: its incidence is increasing and its prognosis is very severe despite the development of new bacteriological identification methods. human beings are contaminated by food, which explains the frequent outbreaks of epidemics which are widely publicized, the in ... | 1993 | 8248080 |
| neglected pathogens: bacterial infections in persons with human immunodeficiency virus infection. a review of the literature (1). | bacterial infections, including those that cause infection in the healthy host as well as those that are more opportunistic, occur very commonly among persons infected with the human immunodeficiency virus (hiv). bacterial infections are a direct result of the severe humoral and cellular immune defects found in these patients. epidemiologic factors such as intravenous drug use and stage of hiv infection may also play important roles. pulmonary, bloodstream, gastrointestinal, central nervous syst ... | 1993 | 8247908 |
| influence of suspension on the expression of protective immunological memory to murine listeria monocytogenes infection. | female bdf1 mice were immunized with virulent listeria monocytogenes, which resulted in the generation of a long-lived state of protective immunological memory for this facultative intracellular bacterial pathogen. the influence of antiorthostatic suspension, a ground-based model employed to simulate certain aspects of weightlessness that occur during spaceflight, on the capacity of these mice to express memory immunity was evaluated. memory-immune mice were suspended by the tail in the orthosta ... | 1993 | 8245710 |
| survival of pathogenic bacteria during mesophilic anaerobic digestion of animal waste. | the survival of pathogenic bacteria was investigated during the operation of a full-scale anaerobic digester which was fed daily and operated at 28 degrees c. the digester had a mean hydraulic retention time of 24 d. the viable numbers of escherichia coli, salmonella typhimurium, yersinia enterocolitica, listeria monocytogenes and campylobacter jejuni were reduced during mesophilic anaerobic digestion. escherichia coli had the smallest mean viable numbers at each stage of the digestion process. ... | 1993 | 8244898 |
| the effect of adult thymectomy on immune response to infection with listeria monocytogenes in mice. | the role of adult thymus in in vivo immune response to infection with listeria monocytogenes was examined using euthymic mice and adult-thymectomized (atx) mice which had been thymectomized 2 weeks before. numbers of t cells in peritoneal cavities and spleens were increased at 2 weeks after inoculation of l. monocytogenes, whereas such increases of t cells were several times higher in euthymic mice than in atx mice. in flow-cytometric analysis of peritoneal exudate cells, a significant increase ... | 1993 | 8244443 |
| comparison of a cold enrichment method and the idf method for isolation of listeria monocytogenes from animal autopsy material. | the method most often used in sweden for isolation of listeria monocytogenes from animal autopsy material is a cold enrichment method. this method is very slow. the international dairy federation (idf) has recently presented a method for detection of l. monocytogenes in milk and milk products that is complete in one week. during a two year period 69 specimens from dead animals with suspected listeriosis were examined for l. monocytogenes in parallel analyses with both the cold enrichment method ... | 1993 | 8236778 |
| serotyping and distribution of listeria isolates from cases of ovine listeriosis. | 1993 | 8236705 | |
| fate of listeria monocytogenes and pediococcal starter cultures during the manufacture of chicken summer sausage. | two formulations of chicken summer sausages [100% hand deboned chicken meat (hdcm) and 85% hdcm and 15% chicken hearts (hdcm-ch)] were prepared with a nonpediocin-producing (ped-) pediococcus acidilactici starter culture and inoculated with 10(4) or 10(7) cfu of a five-strain mixture of listeria monocytogenes/g of batter. sausages were fermented to ph 5.0 (11 h), cooked to an internal temperature of 66.5 c, cold-showered, and stored at 4 c (60 days) and 30 c (7 days). for both formulations and i ... | 1993 | 8234138 |
| mri of an abscess of the cervical spinal cord in a case of listeria meningoencephalomyelitis. | a case of listeria meningoencephalomyelitis is described and the mri appearances of an abscess of the cervical spinal cord are presented. the mri appearances did not allow a definitive diagnosis before death. | 1993 | 8232871 |
| [weakness of cellular response to listeria antigens in pregnant mice]. | it was found that pregnant mice exhibit increased susceptibility to lethal action of listeria monocytogenes, although there was no correlation between increase of susceptibility of animals to lethal effect of bacteria and weakening of elimination of microorganisms from the spleen. increased susceptibility of animals to listeriosis was accompanied by weakening of some parameters of cellular immunity. in pregnant mice of strains c57bl/6, naturally resistant to infections, inhibition of ii-1 by mac ... | 1993 | 8231443 |
| relapsed infection due to listeria monocytogenes confirmed by random amplified polymorphic dna (rapd) analysis. | 1993 | 8228306 | |
| complement receptor type 3 (cd11b/cd18) involvement is essential for killing of listeria monocytogenes by mouse macrophages. | recent work indicated that c receptor type 3 (cr3) mediates most phagocytosis of the facultative intracellular bacterium listeria monocytogenes by mouse macrophages, which can kill it. in contrast, phagocytosis of listeria by a population of nonlistericidal macrophages was largely cr3-independent. these findings suggested that cr3 binding during phagocytosis may be important in determining whether a macrophage kills listeria, or is parasitized by the bacterium. the experiments reported here test ... | 1993 | 8228236 |
| listeria monocytogenes hepatitis in a liver transplant recipient: a case report and review of the literature. | listeria is an uncommon cause of hepatitis in adults. we report the case of a liver transplant recipient who presented with a clinical picture of acute hepatitis, 8 months after grafting. blood cultures yielded listeria monocytogenes. the patient made a full clinical recovery after adequate antimicrobial therapy (ampicillin and gentamicin intravenously for 4 weeks). hepatitis was attributed to the listeria infection. we believe this is the first reported case of listeria hepatitis in an organ tr ... | 1993 | 8228121 |
| risk factors associated with contamination of raw milk by listeria monocytogenes in dairy farms. | a case-control study involving 128 selected dairy farms was conducted to assess the association of several suspected risk factors with the odds of contamination of raw milk by listeria monocytogenes. using logistic regression, we found that poor quality of silage (ph > 4.0), inadequate frequency of cleaning the exercise area, poor cow cleanliness, insufficient lighting of milking barns and parlors, and incorrect disinfection of towels between milkings were significantly associated with milk cont ... | 1993 | 8227616 |
| polarized cells, polar actions. | the recognition of polar bacterial organization is just emerging. the examples of polar localization given here are from a variety of bacterial species and concern a disparate array of cellular functions. a number of well-characterized instances of polar localization of bacterial proteins, including the chemoreceptor complex in both c. crescentus and e. coli, the maltose-binding protein in e. coli, the b. japonicum surface attachment proteins, and the actin tail of l. monocytogenes within a mamm ... | 1993 | 8226658 |
| antibacterial activity of lactobacillus strains isolated from dry fermented sausages. | one hundred strains of lactic acid bacteria isolated from dry cured sausages were tested for antagonistic activity against a set of test strains. nine of 52 strains of lactobacillus casei and three of 48 strains of lact. plantarun produced inhibition zones against the indicator species. the substance excreted by lact. casei crl 705 was active against lact. plantarum, listeria monocytogenes, staphylococcus aureus and a wide range of gram-negative bacteria. the activity of the antibacterial compou ... | 1993 | 8226391 |
| effect of heat treatment on listeria monocytogenes and gram-negative bacteria in sheep, cow and goat milks. | sheep milk, compared with cow and goat milk, had a protective effect on gram-negative bacteria and listeria spp. heated at 65 degrees c in a test-tube method. this effect was not solely due to fat content as cow milk artificially reconstituted to 10% homologous fat was not as protective. listeria monocytogenes in whole sheep, cow and goat milks at an inoculum level of 1 x 10(6) cfu ml-1 was heated at 68 degrees c for 15 s in the plate pasteurizer and survival was only detected in whole sheep mil ... | 1993 | 8226390 |
| purification of helicobacter pylori superoxide dismutase and cloning and sequencing of the gene. | the superoxide dismutase (sod) of helicobacter pylori, a pathogenic bacterium which colonizes the gastric mucosa, evoking a marked inflammatory response, was purified and characterized, and the n-terminal amino acid sequence was determined. the enzyme consists of two identical subunits each with an apparent molecular weight of 24,000. analysis of the primary structure and inhibition studies revealed that h. pylori possesses a typical procaryotic iron-containing enzyme. no other isoenzymes could ... | 1993 | 8225605 |
| activation of the human complement alternative pathway by listeria monocytogenes: evidence for direct binding and proteolysis of the c3 component on bacteria. | the capacity of the intracellular pathogen listeria monocytogenes to activate the alternative pathway of human complement was examined. incubation of l. monocytogenes with human serum in optimal conditions (20% mg2+egta [ethylene glycol-bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid]-chelated serum) consumed (31.3 +/- 3.9)% of c3 hemolytic activity and led to similar amounts of c3 deposition among the 27 strains tested, except for a rough mutant and the penicillin-induced l forms of strai ... | 1993 | 8225590 |
| neutrophils are involved in acute, nonspecific resistance to listeria monocytogenes in mice. | the importance of neutrophils in killing extracellular, pyogenic bacteria has long been established. however, there is only indirect evidence for a role for neutrophils in resistance against intracellular organisms. in this study, we directly demonstrate the involvement of neutrophils in defense against listeria monocytogenes in normal c.b-17 immunocompetent and c.b-17 scid mice. because of the lack of sterilizing t-cell immunity, scid mice are unable to completely eliminate listeriae systemical ... | 1993 | 8225586 |
| cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase c from staphylococcus aureus: a potential staphylococcal virulence factor. | staphylococcus aureus secretes a phosphatidylinositol (pi)-specific phospholipase c (pi-plc) which is able to hydrolyze the membrane lipid pi and membrane protein anchors containing glycosyl-pi. the gene for pi-plc (plc) was cloned from s. aureus into escherichia coli. oligonucleotide probes based on partial protein sequence and polyclonal antibodies raised against the purified protein were used to identify positive clones. e. coli transformed with a plasmid containing the plc gene expressed pi- ... | 1993 | 8225585 |
| production of interleukin 8 (il-8) by cord blood mononuclear cells induced by listeria monocytogenes. | the defective ability of human newborns to mobilize phagocytes to the site of infection led us to examine the ability of cord blood mononuclear cells to secrete interleukin-8, a major neutrophil chemotactic factor, in response to stimulation with listeria monocytogenes. adult or cord blood mononuclear cells were incubated with l. monocytogenes for varying lengths of time, and il-8 was measured in the culture supernatants by the enzyme-linked immunosorbent assay (elisa). spontaneous il-8 secretio ... | 1993 | 8225409 |
| application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of mexico. | this study was conducted at the centre for research, teaching and extension in tropical livestock (centro de investigación, enseñanza y extensión en ganadería tropical) of the faculty of veterinary medicine of the national autonomous university of mexico. during the latter part of 1986 and throughout 1988 and 1989, the herd of holstein x zebu cattle at the university was tested for igg antibodies to twenty-one viral, bacterial, rickettsial and parasitic agents. antigens prepared from twenty infe ... | 1993 | 8219327 |