Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [diagnosis of insect-toxin allergy (bee, wasp) using the radioallergosorbent test]. | 1979 | 425600 | |
| comparison of the atopic background between allergic and non-allergic beekeepers. | a study was carried out on beekeepers and their families. 34 subjects with a history of bee sting allergy and also a positive radioallergosorbent test (rast) to honey bee venom were compared with 47 subjects with neither. 16 subjects in the bee allergy group (47%) had a history of atopic diseases as compared to 6 (13%) in the control group (p less than 0.01). 15 in the bee allergy group (44%) had at least one positive rast to three common inhalant allergens as compared to 5 (11%) in the control ... | 1978 | 422284 |
| relationship between age and hyaluronidase activity in the venom of queen and worker honey bees (apis mellifera l.). | 1979 | 419541 | |
| the effects of chronicity on burst structure in epileptogenic foci. | pyramidal tract neurons (ptns) from tungstic acid, cobalt and alumina gel foci in cat were studied for spontaneous and antidromically-evoked firing patterns. specifically, the structured long-first-interval (lfi) burst was looked for. the lfi burst did occur spontaneously from ptns in tungstic acid foci but could not be antidromically evoked. the lfi burst did not occur as often as would have bee predicted from cobalt and alumina foci, but, in foci older than 60 days, antidromically-evoked activ ... | 1978 | 416881 |
| [mixed "n" type inhibition of alpha-glucosidase activity by glucose in hemolymph of honeybee prenymphs]. | under in vitro inhibition of alpha-glucosidasic activity by glucose in hemolymph of bee prenymphas, the reaction order (n) (predetermined according to the initial natural glycemia) decreases with increasing inhibitor concentration and the affinity constant between enzyme and substrate undergoes lower variations than in other cases where (n) does not change. the study of inhibited molecular forms suggests the possibility of a gradual negative-cooperativity mechanism concerning a dimeric form of t ... | 1977 | 413666 |
| [determination of the kinetic parameters of enzyme inhibition of the k-type mechanism: application to the control of alpha-glucosidase activity of honeybee hemolymph by glucose]. | the alpha-glucosidasic activity of emerging honeybees haemolymph is submitted to a feed-back inhibition by glucose, according to a mechanism of the "k" type (competitive). the "resulting affinity-constant" (measured in the presence of the enzyme both with substrate and inhibitor) is linear function of the inhibitor concentration. the affinity constants between enzyme and pure substrate on one hand, and between enzyme and pure inhibitor on the other hand, were determined by means of this relation ... | 1977 | 409564 |
| [modification of alpha-glucosidases (e.c. 3.2.1.20) activity in honeybee hemolymph during nymphosis]. | the main alpha-glucosidase fraction of honey-bee hemolymph undergoes an important increase of activity for a short time, at a precise stage of nymphosis (pink-brown eyes), without any changes of the apparent-michaelis constant, nor corresponding increase of the protein quantity. | 1977 | 409545 |
| allergens in bee venom ii. two new high molecular weight allergenic specificities. | two new allergenic specificites were detected in honeybee venom and the two corresponding protein substances isolated by gel filtration, immunoadsorption, and ion exchange chromatography. the first of these, allergen b, has a molecular weight ranging from 49,000 to more than 200,00 d and can be recognized by rabbit and guinea pig antisera as well as by human reaginic sera using the radioallergosorbent test (rast). allergen b gives a single line in immunodiffusion distinct from hyaluronidase, pho ... | 1977 | 401840 |
| [varroatosis of the honey bee]. | 1979 | 383454 | |
| quantitative study on anomeric forms of glucose produced by alpha-glucosidases. | anomeric forms of glucose produced from phenyl alpha-maltoside, maltose, or phenyl alpha-glucoside have been determined quantitatively by simultaneous measurements of optical rotation and reducing power, for eight kinds of glucose-producing 1,4-alpha-glucosyl hydrolases, including glucose-forming amylase from human urine, and alpha-glucosidases from pig serum, honey bee, buckwheat seed, rice seed, sugar beet seed, flint corn seed, and brewer's yeast. all the eight enzymes studied were found to p ... | 1979 | 376499 |
| desensitization of patients with bee sting allergy using pure bee venom. | forty patients who had previously experienced severe systemic reactions after a bee sting were desensitized using pure bee venom. a modified 'rush' regimen was employed whereby patients received two injections a week and reached maximal desensitization in 5 weeks. eleven patients have subsequently been stung again and have developed no generalized reaction. although this form of desensitization is considered to be highly effective in protecting sensitive patients, both generalized and local side ... | 1979 | 375434 |
| [size of the genome of the bee wax moth nuclear polyhedrosis virus]. | 1978 | 364252 | |
| [chemistry and pharmacology of bee venom (a review of the literature)]. | 1981 | 350614 | |
| a solid-phase radioimmunoassay for detection of human antibodies. i. measurement of igg antibody to bee venom antigens. | a solid-phase radioimmunoassay (spra) has been developed to measure igg antibodies to bee venom (bv) and phospholipiase a2 (pla) in human sera. the principle of the test is similar to that of the radioallergosorbent test (rast) measuring ige antibody. cyanogen-bromide-activated paper discs coupled with bv or pla followed by supplementary coupling with human serum albumin were incubated with standard or test sera, washed, and incubated with 125i-labeled anti igg. the serum levels of the igg antib ... | 1978 | 344801 |
| the treatment of bee stings. | 1977 | 331296 | |
| biosynthesis of a secretory peptide in honeybee venom glands: intermediates detected in vivo and in vitro. | 1983 | 326578 | |
| radiorespirometric studies of carbohydrate metabolism by washed spermatozoa of various species. | 1. the patterns of 14co2 evolution from specifically labeled glucose substrates by washed bull, ram, boar, rabbit, dog, rooster and turkey spermatozoa were similar and indicated the embden-meyerhof and kreb's cycle pathways as the major route of energy metabolism. 2. honey bee spermatozoa metabolized glucose-3,4-[14c], glucose-[u-14c] or fructose-[u-14c], but not glucose-1-[14c], glucose-2-[14c]or glucose-6-[14c], indicating the presence of the glycolytic pathway, but the absence of respiration ... | 1978 | 318338 |
| comparison of phospholipase activity with direct and indirect lytic effects of animal venoms upon human red cells. | 1. the venoms of 22 species of arthropods, saurians, elapids and crotalids were studied concerning the phospholipase activity and the presence of a direct and an indirect lytic effect upon human red cells. 2. the venoms from the spiders latrodectus and "tarantula", and the venoms from the scorpions of the genus centruroides are not haemolytic and do not have phospholipase activity. 3. only the venoms of apis mellifera and naja naja siamensis have shown direct lytic effect. 4. all other venoms st ... | 1979 | 318305 |
| [capabilities and limitations of in-vitro allergy diagnostics (on the model of bee-venom allergy and on neurodermatitis in relation to ige-t-lymphocyte interaction)]. | 2001 | 311110 | |
| [hyposensitisation treatment with pure bee venom (author's transl)]. | hyposensitisation with pure bee venom was undertaken in eleven persons with proven allergy to bee stings. rapid hyposensitisation led to side effects in seven of them during dosage increase. serial determination of total iga, igg, igm and alpha1-antitrypsin revealed no changes, while allergen-specific igg and ige and total ige increased maximally after 30 days. all persons were exposed to one bee sting during the treatment: all tolerated it well without the allergic reactions which had previousl ... | 1978 | 308876 |
| protection against anaphylaxis in hymenoptera-sensitive patients by passive immunization. | passive administration of specific immunoglobulin led to a transient rise in antiphospholipase igg. an observed rise of 2 microgram/ml (twofold in three cases) was associated with a very diminished response to bee venom. this did not prevent the patient's own igg response to challenge, which was at least as good as that of non-infused patients. the infusion of igg cannot be proposed at present as a routine mode of therapy in insect allergy. however, we present the results of our study as the bes ... | 1977 | 303327 |
| low concentrations of indomethacin inhibit phospholipase a2 of rabbit polymorphonuclear leukocytes. | inhibition of prostaglandin synthesis by indomethacin, a drug with anti-inflammatory properties, has been attributed to its action on fatty acid cyclooxygenase. however, prostaglandin synthesis would also be inhibited if precursor fatty acids were not supplied. we find that indomethacin inhibits phospholipase a2 (phosphatide 2-acylhydrolase, ec 3.1.1.4) of rabbit polymorphonuclear leukocytes in dose-dependent fashion. inhibition is immediate and readily detected at 1 micrometer. the extent of in ... | 1978 | 275865 |
| amino acid sequence of honeybee prepromelittin synthesized in vitro. | translation of melittin messenger rna from queen bee venom glands in a cell-free system from wheat germ yielded prepromelittin. sequence analysis of the labeled in vitro product was performed by automatic edman degradation of the intact polypeptide as well as by analysis of some of its proteolytic fragments. prepromelittin was shown to be composed of 70 amino acids, two of which have not been identified. the sequence of melittin is located in the cooh-terminal third of the polypeptide chain (res ... | 1978 | 273232 |
| concept of internal structural controls for evaluation of inactive synthetic peptide analogs: synthesis of [orn13,14]apamin and its guanidination to an apamin derivative with full neurotoxic activity. | the importance of arginine residues 13 and 14 in the bee venom neurotoxin, apamin, was teste by the synthesis of replacement analogs. [13,14-di-ndelta-trifluoroacetylornithine]apamin was synthesized by the solid phase method on a benzhydrylamine resin. it was deprotected to [13,14-diornithine]apamin, which was then guanidinated to produce the 4-homoarginine-13,14-diarginine analog, [har4]apamin. neither the trifluoroacetylornithine analog nor the ornithine analog produced any detectable symptoms ... | 1977 | 268626 |
| whole body extract versus venom treatment in anaphylactic reactions to bee stings: the doctor's dilemma. | 1990 | 267782 | |
| translation of melittin messenger rna in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide. | melittin messenger rna from queen bee venom glands has been translated in a cell-free system from wheat germ. a product larger than promelittin is formed which has the carboxy-terminal sequence-gln-gln-glycooh. melittin and promelittin from venom glands terminate in -gln-glnconh2. the possible role of the extra glycine residue in the formation of a cooh-terminal amide via a transamidase-like reaction is discussed. | 1993 | 265590 |
| women alcoholics. a review of social and psychological studies. | research on social-history variables, personality characteristics, social roles and role confusion, and possible treatment methods for women alcoholics is reviewed. suggestions for additional studies involving factors motivating drinking in women, theories of personality, new treatment methods, and differentiation between subgroups of women alcoholics are discussed. | 1975 | 240065 |
| comparison of various biogenic amines as substrates for acetyl transferase from apis mellifera (l.) cns. | 1975 | 239818 | |
| the purification and study of a honey bee abdominal sucrase exhibiting unusual solubility and kinetic properties. | 1975 | 237474 | |
| economical bee-sting kits. | 1975 | 236552 | |
| glycerol-3-phosphate dehydrogenase from the honey bee. | 1975 | 236444 | |
| apamin blocks certain neurotransmitter-induced increases in potassium permeability. | apamin is a neurotoxic polypeptide of known structure isolated from bee venom. shuba and coworkers have recently shown that it abolishes the hyperpolarising action of externally-applied atp on visceral smooth muscle (guinea pig stomach and taenia coli) as well as the hyperpolarisation (inhibitory junction potential) that follows stimulation of the non-adrenergic inhibitory nerve supply to these tissues. as it has been proposed that atp is the neurotransmitter involved in the latter response, vl ... | 1979 | 228203 |
| phospholipid changes in synaptic membranes by lipolytic enzymes and subsequent restoration of opiate binding with phosphatidylserine. | a study has been made of the role of phosphatidylserine in stereospecific opiate binding to neural membranes, utilizing specific lipolytic enzymes to attack the lipid. at very low concentrations phospholipase a2 from bee venom will preferentially hydrolyze c22:6-fatty acid; and even after a few percent of the total phosphatidylserine is hydrolyzed, opiate binding is greatly inhibited. the addition of brain phosphatidylserine will restore opiate binding; however, when the inhibition approaches 50 ... | 1978 | 210831 |
| testing blood plasma from cattle in leukosos-free herds for antibodies against bovine leukemia virus (blv). | as a prelude to the introduction of serological methods in the danish leukosis eradication programme, an examination for antibody to bovine leukemia virus (blv) was carried out in 215 randomly selected leukosis-free herds in three areas where 3 routine haematological screening tests had bee made over a period of 6 years. a gel diffusion test was applied to plasma samples. of 3319 animals screened, none were found to have detectable levels of antibody to blv in their plasma. this would seem to in ... | 1978 | 208054 |
| immunoassay for honey bee cytochrome c in single animals with cytochrome c-coated bacteriophages: a sensitive tool for the study of caste formation in the honey bee, apis mellifera. | the development of a sensitive viroimmunoassay for honey bee cytochrome c and its usage for early detection of caste differentiation is described. pure honey bee cytochrome c was isolated from workers and used to produce antibodies in rabbits. bacteriophage t4 was chemically modified by covalent attachment of honey bee cytochrome c using tolylene-2,4-diisocyanate as a cross-linking agent. the immunospecific inactivation of this bacteriophage-cytochrome c conjugate by anti-cytochrome c antibodies ... | 1977 | 189841 |
| gonadotropin receptors in plasma membranes of bovine corpus luteum. i. effect of phospholipases on the binding of 125i-choriogonadotropin by membrane-associated and solubilized receptors. | the ability of bovine corpus luteum plasma membranes to bind 125i-choriogonadotropin has been examined after prior treatment of the membranes with phospholipases a, c, and d. treatment of the purified membranes with low concentrations of phospholipases a and c resulted in the inhibition of the binding of 125i-choriogonadotropin to its receptors, whereas phospholipase d had no effect. receptor activity was decreased by low concentrations of phospholipase a from either bee venom, vipera russelli o ... | 1976 | 187585 |
| substrate cycles in metabolic regulation and in heat generation. | 1. the presence of substrate cycles in tissues has been demonstrated by direct isotope methods in recent years. this demonstration has provided the impetus for a reappraisal of the roles of substrate cycling in metabolic regulation and in heat production. these aspects of substrate cycling are discussed in this paper. the relationship between near-equilibrium reactions and substrate cycles is emphasized, since this provides a basis for the derivation of a function describing in precise quantitat ... | 1976 | 184791 |
| evidence for a phospholipid requirement in the specific binding of glucocorticoids to receptors of fibroblasts and thymic lymphocytes. | the specific steroid binding capacity of soluble preparations from mouse fibroblasts and rat thymic lymphocytes is inactivated by incubation with phospholipases. receptor binding is drastically reduced by very low concentrations of boiled phospholipase a preparations from bee venom and snake venoms. the enzyme effect is calcium-dependent and is blocked by both phospholipid and a substrate analog that is a competitive inhibitor of phospholipase a. the specific binding capacity is also sensitive t ... | 1976 | 177409 |
| organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases. | 1. the action of eight purified phospholipases on intact human erythrocytes has been investigated. four enzymes, e.g. phospholipases a2 from pancreas and crotalus adamanteus, phospholipase c from bacillus cereus, and phospholipase d from cabbage produce neither haemolysis nor hydrolysis of phospholipids in intact cells. on the other hand, both phospholipases a2 from bee venom and naja naja cause a non-haemolytic breakdown of more than 50% of the lecithin, while sphingomyelinase c from staphyloco ... | 1975 | 169915 |
| sensitive assay for detection of toxin-induced damage to the cytoplasmic membrane of human diploid fibroblasts. | a sensitive assay was developed for detection and quantitation of subtle permeability changes in the cytoplasmic membrane of human diploid fibroblasts. release of the non-metabolizable amino acid [1-14c]alpha-aminoisobutyric acid (aib; molecular weight (103) from the cytoplasm of prelabeled cells was used as an indicator of toxin-induced membrane damage. an optimal procedure for labeling these cells was designed after varying the conditions with regard to ph, temperature, concentration of aib, c ... | 1975 | 169201 |
| depressed in vitro aggregation of platelets of chronic hemodialysis patients (chdp): a role for cyclic amp. | 1) on log-log-coordiantes a direct correlation has bee shown to exist between plasma cyclic amp and plasma cr levels. 2) hemodialysis results in a significant reduction in the arterial plasma cyclic amp levels, but a return of plasma cyclic amp to pre-dialysis levels is seen within 30 mins post-dialysis. 3) the dialyzer clearance of cyclic amp, both in vitro and vivo, is commensurate with its m.w. 4) in vitro platelet aggregation responses, to adp, epi, and coll are not influenced by prp cell co ... | 1975 | 167491 |
| neuromuscular block in honeybees by the venom of the bee wolf wasp (philanthus triangulum f.). | 1995 | 167475 | |
| triosephosphate dehydrogenase from the honey bee. | 1975 | 165383 | |
| determination of toxin-induced leakage of different-size nucleotides through the plasma membrane of human diploid fibroblasts. | human diploid lung fibroblasts were treated with cytolytic bacterial toxins and the nature of the membrane damage was investigated. [3h] uridine was used for differential labeling of cytoplasmic components of small or large molecular size. two principal size categories were achieved by labeling the fibroblasts in either early growth phase or stationary phase, a high-molecular weight ribonucleic acid label and a low-molecular-weight nucleotide label. the size of the labeled molecules was determin ... | 1975 | 164404 |
| [kinetic properties of an alkaline phosphatase from honeybee intestine determined by a new rigorous algebraic method]. | the hill-parameters : max. velocity (vm), affinity constant (k) and resultant reaction order (n) of an alk. phosphatase (mol. weight 100 to 130,000) from guts of foraging worker-bees, have been calculated according to new algebraic equations allowing a rigorous determination without any extrapolation. the parameters determined from 2 strains of the same race, at 3 years interval, do not differ, and the hill coefficients (n) are significantly lower than 1, namely : n = 0.621 +/- 0.014 (3 detn.) a ... | 1978 | 157198 |
| [studies on the rectal pads of the honey bee apis mellifca]. | according to earlier investigations young bees show a rectal fluid hypo-osmotic to the haemolymph. it had been assumed, however, that in certain situations honey bees have to be economical with water and simultaneously form a rectal fluid hyperosmotic to the haemolymph. the latter has been confirmed by the present investigation. | 2016 | 134591 |
| effects of caffeine and various xanthines on hornets and bees. | the effect of caffeine was assessed on vespa orientalis hornets maintained either in sealed breeding boxes or as entire colonies free to forage, and also on apis mellifera bees within their hives. in a number of instances the hornets were also used to study the effect of various bodily extracts of queen hornets and of the following xanthines: purine; hypoxanthine; uric acid; theophylline; and theobromine. the studied materials were found to exert an effect on three categories of activities: (1) ... | 2002 | 117503 |
| [in vitro inhibition of trna methyltransferases by queen substance, a pheromone of queen honeybees]. | the queen substance 1, a pheromone of the queen honeybee apis mellifica is an in vitro inhibitor of e. coli b trna methylations. this activity is not specific of the methylase source, as inhibitions have been observed with preparations from queen honeybee ovaries, rat liver or a mouse plasmocytoma 1-adenine methylase. these results, together with preceding ones concerning t, t-farnesyl-acetone 3, are discussed. | 1979 | 113116 |
| isolation of the cytoplasmic form of malate dehydrogenase from honey bee (apis mellifera) larvae. | 1979 | 111673 | |
| [on the antimicrobial activity of propolis and propolis constituents (author's transl)]. | after a survey of the literature on the antimicrobial activity of the bee product propolis, the authors discuss their own findings as compared to the chemotherapeutical agents streptomycin, oxytetracycline, chloramphenicol, nystatin, griseofulvin and sulphamerazine. according to the results obtained by testing 25 isolated constituents on bacillus subtilis, staphylococcus aureus, candida albicans and trichophyton mentagrophytes, the antimicrobial properties of this mixture of natural substances a ... | 1979 | 108687 |
| immunologic and biochemical evaluation of the potency of whole insect body extracts. | recent studies have indicated that currently available whole body extracts have little potency and are ineffective for diagnosis and treatment of stinging insect allergy. pure venom is a potent effective allergen but is difficult to obtain in sufficient quantities from all hymenoptera species. in these studies, an attempt was made to prepare a potent whole body extract. whole bee body extracts were prepared with different extraction periods and at cold and room temperatures. potency was examined ... | 1979 | 107209 |
| activation of high levels of endogenous phospholipase a2 in cultured cells. | activatable cellular phospholipase a2 (plase; phosphatide 2-acyl-hydrolase, ec 3.1.1.4) has been proposed to constitute the first and rate-limiting step in prostaglandin synthesis and to regulate membrane function by altering the levels in the membrane of the detergent lipids lysolecithin and free fatty acids. we have observed that a wide variety of cells in culture contain high levels of endogenous plase that can be activated by polypeptide toxins, such as melittin purified from bee venom and d ... | 1979 | 106389 |
| studies on the characterization of the rho(d) antigen. | the rho(d) antigen of red cell membranes was solubilized using ethylene-diamine tetraacetic acid (edta) and 2-mercaptoethanol. the solubilized antigen was partially separated from other solubilized membrane components using molecular filtration. the antigen was treated with various enzymes to learn some of the chemical characteristics. it was found that the activity of the antigen, as measured by hemagglutination inhibition, was not affected by bee venom phospholipase a, clostridium welchii phos ... | 1978 | 103579 |
| accessibility of phospholipids in the chromaffin granule membrane. | 1. the accessibility of phospholipids in the membrane of the adrenomedullary storage vesicles (chromaffin granules) has been studied. 2. the reaction of 2,4,6-trinitrobenzenesulphonic acid with both intact granules and their ghosts, results in the labelling of 70% of the phosphatidylethanolamine. 3. the action of phospholipase a2 (from bee venom), phospholipase c (from bacillus cereus) and sphingomyelinase c (from staphylococcus aureus) on granules and their ghosts was followed as a function of ... | 1978 | 102348 |
| spiroplasmas: serological grouping of strains associated with plants and insects. | spiroplasma strains from plant and arthropod hosts, and from surfaces of flowers, were classified into three serological groups (designated i, ii, and iii) based on results from growth-inhibition tests. no significant cross reactions were observed among groups. the groupings were confirmed by ring-interface precipitin and microprecipitin tests, using membrane preparations as test antigens, and by organism-deformation tests. serogroup i contained three subgroups: subgroup a (spiroplasma citri str ... | 1979 | 93504 |
| further studies on the structural requirements for polypeptide-mediated histamine release from rat mast cells. | structure-activity studies have been performed on a series of naturally occurring and 'tailor-made' polypeptides, by measurement of ability to induce selective histamine release from normal rat peritoneal mast cells in vitro. compounds investigated include corticotropin and melittin derivatives, mast-cell-degranulating peptide from bee venom, polymyxin b, bradykinin and various synthetic poly(amino acids) and short-chain peptides. it was confirmed that a cluster of four basic residues (lysine or ... | 1979 | 92986 |
| lipolytic enzymes in bovine thyroid tissue. iii. lysophospholipase activity. | lysophospholipids are formed during phospholipid breakdown as a result of the action of phospholipases a. at certain concentrations these lysoderivatives destabilise biological membranes. therefore, their concentration is of critical importance for membrane integrity. prevention of lysophosphoglycerides accumulation may be the important role for lysophospholipases and is probably the explanation for their widespread occurrence in nature. lysophospholipase activities were found in molds (fairbair ... | 1979 | 92222 |
| effect of alamethicin, gramicidin s and melittin upon the particulate guanylate cyclase from rat lung. | the channel-forming antibiotic alamethicin activated rat lung particulate guanylate cyclase (gtp pyrophosphate-lyase (cyclizing) ec 4.6.1.2), and the activated enzyme was further stimulated by sodium nitroprusside when a thiol such as 2-mercaptoethanol was present. similar effects were seen with the antibiotic gramicidin s and with melittin, a polypeptide purified from bee venom. all of these agents are amphiphilic polypeptides. nitroprusside was not able to stimulate both particulate and solubl ... | 1979 | 90524 |
| [allergy and histamine release (author's transl)]. | histamine is released from mastocytes and basophils polymorphonuclears. this occurs particularly in reaginic type of allergy. the different phases of the reaginic process has been studied: ige bridgings with antigens, cell membrane activation, cytoplasmic biochemical reactions involving cyclic amp and gmp leading to microtubules activities, contraction of microfibrils and degranulation. calcium and magnesium contribute to the regulation of histamine release. histamine releases could also be seen ... | 2000 | 89696 |
| solid phase radioimmunoassay for quantitation of antigen-specific igg in human sera with 125i-protein a from staphylococcus aureus. | radiolabeled protein a from staphylococcus aureus (staph a) has been used to develop a solid phase, noncompetitive radioimmunoassay for quantitation of specific igg antibody. the assay involves two incubations: first, agarose-insolubilized antigen is mixed with serum samples for 1 to 4 hr during which specific antibody is bound; second, after a washing procedure, the solid phase immune complexes are incubated for 4 to 18 hr with 125i-staph a, during which the radiolabeled detection protein binds ... | 1979 | 87416 |
| lipolytic enzymes in bovine thyroid tissue. i. subcellular localization, purification and characterization of acid phospholipase a1. | in mammalian cells the catabolism of membrane phosphoglycerides proceeds probably entirely through a deacylation pathway catalysed by phospholipase a and lysophospholipase (wise & elwyn, 1965). in the initial attack of diacylphosphoglycerides by phospholipase a two enzymatic activities with different positional specificities have been distinguished: phospholipase a1 (phosphatidate 1-acyl hydrolase en 3.1.1.32) and phospholipase a2 (phosphatidate 2-acyl hydrolase en 3.1.1.4) (van deenen & de haas ... | 1978 | 87159 |
| [the presence of conjugated noradrenaline in the wax from the honey comb of apis mellifera linne (proceedings)]. | 1977 | 79374 | |
| effects of passive antibody in bee venom anaphylaxis. | twelve patients allergic to honeybees were challenged by injections of bee venom; five responded systemically to the venom, with symptoms ranging from angioedema to respiratory distress. these patients were given intramuscular or intravenous infusions of gamma-globulin obtained from the plasma of hyperimmune beekeepers who had high levels of antibody to an allergen (phospholipase a) in the venom. post-infusion, all five patients tolerated 1.5 to 5 times the venom dose that previously elicited ad ... | 1978 | 75280 |
| polistes wasp hypersensitivity: diagnosis by venom-induced release of histamine in vitro. | polistes wasps cause a majority of hymenoptera-induced anaphylactic reactions in texas. using the in vitro release of histamine from basophils of patients allergic to polistes stings, we have studied the cross-reactivity of venoms from three species of polistes wasps as well as the cross-reactivity among polistes, honeybee, and vespula maculifrons (yellow jacket) venoms. venom collected by an extrusion technique from pollistes exclamans, pollistes apachus, and pollistes carolina caused release o ... | 1977 | 71310 |
| a high molecular weight allergenic fraction of honeybee venom. | chromatography of honeybee venom on sephadex g-150 super fine revealed a high molecular weight (hmw) fraction that elutes prior to hyaluronidase (hyal) and comprises 2% to 4% of the venom weight. hmw appears to exist in polymeric form, and the polymer which is present in greatest concentration has an estimated molecular weight of 105,000 d. the 12% nitrogen content of hmw suggests it may not be all protein. hmw is antigenically and enzymatically distinct from hyal and phospholipase a2 (phos a). ... | 1977 | 70436 |
| [histamine-liberating action of mcd-peptide from bee venom]. | histamine release (hr) from the isolated rat mast cells induced by mcd-peptide, mellitin (from the apis mellifica venom) and compound 48/80 was studied. the dose-response curve, the latent period and temperature dependence of hr induced by mcd-peptide were similar to those of hr induced by compound 48/80. the hr induced by mcd-peptide proved to be an energy-dependent process that was independent of cyclic adenosine-3',5'-monophosphate. | 1977 | 70248 |
| detoxified hymenoptera venoms: preliminary studies of in vitro cytotoxicity and antigenicity of apis mellifera 'venomoid'. | the preparation of detoxified venom (venomoid) of apis mellifera is described. the venomoid obtained by formaldehyde detoxification retained significant antigenicity. radioallergosorbent test (rast) inhibition studies indicated a loss of the affinity of the venomoid for the venom-specific human ige antibody. in rabbits the venomoid was capable of producing precipitating antibodies which cross-reacted with the original venom. cytotoxic activity of venomoid as measured by nonimmunological histamin ... | 1977 | 67088 |
| [presence of free and conjugated noradrenaline in bee honey]. | 1976 | 65965 | |
| diagnosis of hymenoptera hypersensitivity by skin testing with hymenoptera venoms. | this double-blind study provides evidence that skin testing with dialyzed hymenoptera venoms is a more accurate, reliable method of diagnosing hypersensitivity to the sting of honeybee, yellow jacket, yellow hornet, white-faced hornet, and wasp than is skin testing with the corresponding whole body diagnostic allergenic extract. furthermore, the incidence of false-positives was greatly reduced by using the dialyzed hymenoptera venom (hdv) diagnosis. in this clinical trial, most sensitive individ ... | 1977 | 64482 |
| harvester ant sensitivity: in vitro and in vivo studies using whole body extracts and venom. | harvester ant stings by pogonomyrmex maricopa (pm) or pogonomyrmex rugosus (pr) resulted in serious reactions in 8 patients, 4 with generalized reactions and 1 with large local reactions. exposure to one species in the genus pogonomyrmex (p) appeared to cross-sensitize ant-sensitive patients to other species in the same genus as evidenced by skin testing and leukocyte histamine release, but these patients were less sensitive to extracts from other stinging hymenoptera, including bee, wasp, yello ... | 1977 | 64481 |
| studies of the antigenicity and allergenicity of phospholipase a2 of bee venom. | the antigenic and allergenic properties of phospholipase a2 (pla2) and whole bee venom were compared by measuring the igg and ige antibody responses in animals and man. precipitating antibodies raised in rabbits and reaginic and other antibodies raised in mice reacted about equally with both bee venom and pla. the majority of human sera containing bee venom-specific ige also contained pla-specific ige, although in somewhat lower titers. similarly, most human sera with significant amounts of tota ... | 1976 | 59745 |
| comparison of the allergenic properties of bee venom and whole bee body extract. | the allergenic properties of bee venom and whole bee body extract were compared by in vivo and in vitro tests. the majority of patients with known bee sting sensitivity had positive intracutaneous skin test reactions with bee venom and had bee venom specific ige in their sera. of seventeen patients with ppsitive bee venom skin tests, nine had positive tests with whole bee body extract. of thirty sera containing elevated levels of bee venom specific ige obtained from untreated patients, fourteen ... | 1976 | 59640 |
| diagnosis of allergy to stinging insects by skin testing with hymenoptera venoms. | skin testing was done on 30 patients with a history of anaphylactic reactions after a hymenoptera sting and on 30 control subjects. the patients all had positive basophilhistamine release to one or more venoms on challenge with the specific venoms used for skin testing (honey bee, yellow jacket, white-faced hornet, yellow hornet, and polistes). at 0.1 mug of venom/ml and at 1.0 mug of venom/ml, 75% and 100%, respectively, of the sensitive patients had a positive skin test. there was a significan ... | 2000 | 59564 |
| an iridovirus from bees. | an iridovirus, apis iridescent virus (aiv), isolated from sick adult specimens of apis cerana (hymenoptera) from kashmir, closely resembles iridescent viruses from tipula and sericesthis spp. (tiv and siv). however, aiv is only distantly related serologically to tiv and siv and is even more remotely related to several other similar viruses that were tested in tube precipitation tests with intact particles. aiv multiplies in apis mellifera, forming cytoplasmic iridescent crystalline aggregates in ... | 1976 | 58966 |
| measurement of igg-blocking antibodies: development and application of a radioimmunoassay. | a radioimmunoassay for measuring blocking antibodies has been developed. we used the ragweed antigen e system to show that the same blocking antibodies (igg) measured by inhibition of antigen-induced leukocyte histamine release were precipitated in the binding assay (r8 = 0.96, p less than 0.001), thus validating a widely applicable technique for measuring blocking antibodies. binding of phospholipase-a (phos-a), the major allergen in honey bee venom, was also shown to correlate significantly wi ... | 2000 | 58936 |
| allergens of honey bee venom. | 2001 | 56920 | |
| allergy to insect stings. ii. phospholipase a: the major allergen in honeybee venom. | in order to determine the proteins of major allergenic importance in honeybee venom (apis mellifera) it was chromatographed on g-50 sephadex. the four major protein peaks eluted were identified as hyaluronidase, phospholipase, melittin, and apamin. testing these preparations on the leukocytes of 6 honeybee-sensitive patients, with the in vitro method of histamine release, revealed that all individuals were most sensitive to phospholipase a. ige antibodies against phospholipase a (rast) were foun ... | 1976 | 54382 |
| golgi studies of the first optic ganglion of the ant, cataglyphis bicolor. | the neurons of the first optic ganglion (the lamina) in the desert ant, cataglyphis bicolor, have been studied with the light microscope after golgi silver impregnation. the different types of retinal and laminal fibres and their configuration are compared with the results obtained in the bee. the first synaptic region in the visual system of the ant lies proximally to the fenestrated layer below the basement membrane and the layer containing the monopolar cell bodies. the synaptic region can be ... | 1975 | 50141 |
| effect of phospholipase a2 on purified gastric vesicles. | the phospholipid and fatty acid composition and role of phospholipids in enzyme and transport function of gastric (h+ + k+)-atpase vesicles was studied using phospholipase a2 (bee venom). the composition (%) was phosphatidyl-choline (pc) 33%; sphingomyelin (sph) 25%; phosphatidylethanolamine (pe) 22%; phosphatidylserine (ps) 11%; and phosphatidylinositol (pi) 8%. the fatty acid composition showed a high degree of unsaturation. in both fresh and lyophilized preparations, even with prolonged incub ... | 1979 | 44534 |
| the kinetic parameters of trehalase in whole and disrupted mitochondrial preparations from two insects with asynchronous muscle. | the kinetic parameters of trehalase in honey bee and flesh fly mitochondria were compared. the studies were carried out with whole mitochondria and with mitochondria disrupted in various ways and to different degrees. honey bee mitochondrial trehalase was significantly activated by lubrol wx treatment (30.0-fold), by high ph treatment (20.8-fold), and by a treatment consisting of 10 passes through a french press (37.9-fold) but not by the other treatments tried (salt, proteases, waring blender, ... | 1979 | 44213 |
| [effect of neurotoxins on mediator release from the motor nerve endings of the drosophila melanogaster larva]. | spontaneous miniature junctional potentials and induced by motor nerve stimulation junctional potentials were recorded in muscle fibres of the third instar drosophila larvae. presynaptically active neurotoxins isolated from the snake venoms (beta-bungarotoxin 4.5 x 10(-7) m, notexin and notechis-ii-51 x 10(-7) m, crotoxin 9 x 10(-8) m, phospholipase from the venom of cobra 4 x 10(-6) m did not induce any changes in transmitter release during 60--120 min of contact with drosophila preparation, al ... | 2014 | 42240 |
| arms races between and within species. | an adaptation in one lineage (e.g. predators) may change the selection pressure on another lineage (e.g. prey), giving rise to a counter-adaptation. if this occurs reciprocally, an unstable runaway escalation or 'arms race' may result. we discuss various factors which might give one side an advantage in an arms race. for example, a lineage under strong selection may out-evolve a weakly selected one (' the life-dinner principle'). we then classify arms races in two independent ways. they may be s ... | 1979 | 42057 |
| enzymes of bee venom, sac and whole body. | by using the api-zym system 55 enzymes were determined to be present in bee venom, venom sac, sacless whole body extract and whole body commercial extracts. acid phosphatase activity was high in bee venom and sacless whole body extracts. arylamidase activities were elevated in sacless whole body extracts and very low or undetectable in venoms. conversely, glycyl-prolin arylamidase activity was high in venoms and very low in the other extracts. | 1979 | 37787 |
| [effect of presynaptic neurotoxins from the bee and cobra venoms on spontaneous mediator secretion from the motor nerve endings in mice]. | phospholipases a2 (phla) from the bee and cobra venoms induced the three-phasic changes in miniature end-plate potential (mepp) frequency recorded in the mouse diaphragm muscle: an initial fall of transmitter release followed by a transient increase before the final complete blockade. the removal of ca2+ from the perfusing solution (below 10(-9) m) prevented the presynaptic effect of both phla. if all phla molecules were washed out by the ca-free solution, the subsequent exposure to a standard s ... | 1979 | 36937 |
| inhibition of beta-bungarotoxin action by bee venom phospholipase a2. | 1998 | 24852 | |
| phospholipases: melittin facilitation of bee venom phospholipase a2-catalyzed hydrolysis of unsonicated lecithin liposomes. | 1977 | 20842 | |
| the organization of perpendicular fibre pathways in the insect optic lobe. | high resolution serial photomicrography has been used to plot the axonal projection patterns between retina, lamina and medulla in the optic lobes of various insects with differing ommatidial receptor arrangements. observations are reported on the cabbage white and skipper butterflies, the bee, locust, fly, backswimmer and waterbug. the patterns of these fibre pathways have previously eluded non-rigorous analyses primarily because of their physical dimensions but are revealed in this study to ha ... | 1976 | 11512 |
| oxygen consumption and thermoregulation in apis mellifera workers and drones. | 1976 | 9250 | |
| temperature maintenance and co2 concentration in a swarm cluster of honey bees, apis mellifera. | 1976 | 7416 | |
| physical, chemical, and enzymatic studies on the major sucrase of honey bees (apis mellifera). | a sucrase from honey bees (apis mellifera) which precipitates between ammonium sulfate saturations of 50 and 70% (5 mg protein per millilitre) and which makes up the major portion of the sucrases of honey bees was purified to homogeneity as shown by several criteria. a large part of the sucrase was found in the head while most of the rest was in the abdomen (a small amount was in the thorax). the enzyme precipitated between the same values of ammonium sulfate saturation as did the sucrase in hon ... | 1976 | 4203 |
| properties of a free and a solubilized form of bound alpha,alpha-trehalase purified from honey bee thorax. | the free and bound forms of alpha,alpha-trehalase (ec 3.2.1.28) of the honey bee thorax were separated and the bound enzyme was solubilized by raising the ph to 8.0 for 10 h. both enzymes were purified. they were homogeneous as determined by several electrophoretic criteria. it was found that the two enzymes had very similar km's (each about 0.89 mm), vm's (53.2 and 54.3 u/mg for free and solubilized, respectively), inhibition characteristics, specificities (both only hydrolyzed alpha,alpha-treh ... | 1975 | 1144 |
| phospholipases. iii. effects of ionic surfactants on the phospholipase-catalyzed hydrolysis of unsonicated egg lecithin liposomes. | apparent values of km and vmax have been measured for catalysis of hydrolysis of unsonicated egg lecithin liposomes, activated through addition of 0.4 m n-hexanol, by phospholipases a2 from bee and snake venoms and by phospholipase c from clostridium welchii as a function of the concentration of three surfactants: hexadecylamine, hexadecyltrimethylammonium bromide, and dihexadecyl phosphate. for all three enzymes, values of km and vmax show little or no dependence on the concentration of these i ... | 1975 | 506 |