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interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...19852410445
vertical transmission of enteric pathogens at birth.to determine how frequently mothers infect their infants with enteric pathogens at birth, stools were collected from 75 thai mothers immediately before delivery and from their infants 24-48 h later. enteropathogenic escherichia coli (epec) were isolated from 25 of the 75 mothers just prior to delivery (32%), enteroviruses from six (8%), enterotoxigenic e. coli from five (7%), and shigella, salmonella and vibrio parahaemolyticus each from one (1%). epec serotype 0128:k67 was isolated from one mot ...19852409892
epidemiology of travelers' diarrhea and relative importance of various pathogens.each year 12 million persons travel from an industrialized country to a developing country in the tropics or subtropics. these travelers experience a high rate of diarrhea caused by a wide variety of enteric pathogens acquired by ingestion of contaminated food or water. one or more pathogens can be found in the stool of a majority of ill individuals. enterotoxigenic escherichia coli generally are the most frequently identified pathogens, having been found in a median of 42% of travelers' diarrhe ...19902406860
diarrhea in developed and developing countries: magnitude, special settings, and etiologies.diarrheal diseases are major causes of morbidity, with attack rates ranging from two to 12 or more illnesses per person per year in developed and developing countries. in addition, diarrheal illnesses account for an estimated 12,600 deaths each day in children in asia, africa, and latin america. the causes of diarrhea include a wide array of viruses, bacteria, and parasites, many of which have been recognized only in the last decade or two. while enterotoxigenic escherichia coli and rotaviruses ...19902406855
microbiological aspects of helicobacter pylori (campylobacter pylori).the human gastric pathogen campylobacter pylori has recently been reclassified as helicobacter pylori, and a related spiral bacterium found in the stomach of ferrets has been designated helicobacter mustelae. the general microbiological features of helicobacter pylori are delineated here, with details of phenotypic differences between helicobacter pylori and helicobacter mustelae; comparisons are made with wolinella succinogenes and campylobacter jejuni. the helicobacter organisms possess an ext ...19902406141
serogroups of campylobacter jejuni from man and animals.a total of 186 campylobacter strains from aborted calf and sheep fetuses, from scouring dogs, rabbits and man, and from retailed poultry were isolated and examined biochemically and serologically for heat stable antigens. immune sera were produced in rabbits against penner reference strains from 1 to 60, and against two field isolates. out of 186 biochemically tested strains 179 (96.2%) proved c. jejuni and only 6 (3.2%) c. coli. one strain has been identified as c. laridis. in cattle and sheep ...19902402970
use of non-radioactive dna probes for detection of campylobacter jejuni and campylobacter coli in stool specimens.dna probes specific for c. jejuni (pdt1720 containing a 1475 base pair fragment) and for c. jejuni and c. coli (pdt1719 containing a 1845 base pair fragment) were isolated from a bacteriophage lambda gt11 genomic library of c. jejuni, using antiserum prepared against a 46 kda major outer membrane protein of c. jejuni. the two probe-fragments had different restriction maps and were only moderately related by dna hybridization analysis. a non-radioactive labelling kit which consisted of alkaline p ...19902402249
invasion-related antigens of campylobacter jejuni.a hep-2 cell culture model was used to investigate the antigens required for epithelial cell penetration by campylobacter jejuni. penetration of hep-2 epithelial cells by c. jejuni was significantly inhibited (p less than .05) with c. jejuni lysate and a monoclonal antibody (mab 1b4) in competitive inhibition assays. immunogold electron microscopy revealed that mab 1b4 bound to the flagella and cell surface of low-passage (invasive) c. jejuni m 96, whereas only the flagella of high-passage (noni ...19902401792
[clinical evaluation of norfloxacin in pediatrics].we have evaluated the effectiveness and safety of norfloxacin (nflx) in 18 children with infectious diseases. doses ranging from 5.6 to 18.8 mg/kg/day for t.i.d. or q.i.d. were used. the causative bacteria were campylobacter jejuni in 3 cases, salmonella typhimurium in 1, staphylococcus aureus in 2, haemophilus influenzae in 1 and unknown in 11 cases. except 1 strain of s. aureus, all the bacteria mentioned above were eradicated. clinical effects were excellent in 8, good in 8, poor in 2, and th ...19902391762
[clinical effect of norfloxacin in pediatric field].we have investigated bioavailability, clinical efficacy, side effect and antimicrobial activity of norfloxacin (nflx) to evaluate the efficacy and safety in the pediatric field. results are summarized as follows 1. after oral administration of 100 mg, tmax was 2 hours and t 1/2 were 4.2 and 2.6 hours in 2 cases. 2. in 10 cases of urinary tract infection (uti) and 4 cases of intestinal tract infection (iti), clinical efficacy rate was 100% (14/14). 3. in uti, causative bacteria were all eradicate ...19902391759
[bacteriological and clinical studies on norfloxacin in the field of pediatrics].bacteriological and clinical studies on norfloxacin (nflx) were carried out in the field of pediatrics, and the results obtained are summarized as follows: 1. the mics of nflx against clinically isolated organisms were determined to compare with mics of nalidixic acid, amoxicillin, cefaclor, erythromycin and fosfomycin. nflx had low mics against both gram-positive and gram-negative bacteria and, particularly, showed higher antimicrobial activity to escherichia coli, salmonella sp., klebsiella pn ...19902391755
[laboratory and clinical studies on norfloxacin in pediatrics].norfloxacin (nflx, am-715) is a new quinolone antibacterial agent. in the present study, we carried out laboratory and clinical investigations on nflx in the field of pediatrics. the obtained results are summarized as follows. 1. nflx was very active against escherichia coli, salmonella sp., klebsiella sp., enterobacter sp. and pseudomonas aeruginosa among gram-negative rods. antibacterial activities of nflx against gram-positive cocci were lower than those against gram-negative rods but superio ...19902391753
[laboratory and clinical studies on norfloxacin in the pediatric field].in bacteriological, pharmacokinetic and clinical studies on norfloxacin (nflx, am-715), the following results were obtained: 1. antibacterial activity of nflx, nalidixic acid (na), amoxicillin (ampc), cefaclor (ccl), erythromycin (em) and fosfomycin (fom) against clinically isolated bacteria was examined, and it was found that mic80 of nflx against staphylococcus aureus was 3.13 micrograms/ml, thus nflx exhibited stronger antimicrobial activity than na, ampc, ccl, em and fom. nflx also showed go ...19902391752
[clinical evaluation of norfloxacin in children].oral new quinolone, norfloxacin (nflx, am-715), was evaluated for its safety, efficacy and pharmacokinetics in children. 1. nflx was effective in 88.0% of 25 cases infected with haemophilus influenzae, pseudomonas aeruginosa, escherichia coli, campylobacter jejuni, staphylococcus aureus including methicillin-resistant strains, and other bacteria. 2. after single oral administration of 50 mg and 100 mg nflx tablet at fasting, mean peak values of serum concentration were 0.35, 0.48 microgram/ml an ...19902391750
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...19902387622
investigation of seemingly pathogen-negative diarrhoea in patients infected with hiv1.thirty three consecutive patients infected by human immunodeficiency virus type 1 (hiv1) with persistent diarrhoea which remained undiagnosed after microbiological examination of six stool samples and rectal histology were investigated for malabsorption. all had xylose and schilling tests, distal duodenal biopsy, comprehensive barium studies, microbiological examination of six further stool samples, and repeat rectal histology. a microbiological or histological diagnosis of infection was made in ...19902387512
in vitro susceptibility of campylobacter jejuni to rokitamycin.the susceptibilities of 100 clinical isolates of campylobacter jejuni to rokitamycin, a new macrolide, and eight other oral antibiotics were tested by using a broth microdilution method. rokitamycin demonstrated antibacterial activity that was higher than those of fosfomycin and the tetracyclines and comparable to those of erythromycin and clindamycin, whereas it was slightly less active than the quinolones.19902386373
isolation of campylobacter jejuni from the eggs and organs in experimentally infected laying japanese quails (coturnix coturnix japonica). 19902385046
peritonitis caused by campylobacter jejuni and serologically confirmed in a patient being treated with continuous ambulatory peritoneal dialysis.a case of campylobacter jejuni peritonitis in a patient being treated with continuous ambulatory peritoneal dialysis is described. the significance of the organism isolated from our patient was initially doubtful because of a rapid defervescence of symptoms with minimal specific antibiotic therapy, lack of a preceding diarrhoeal illness and the time taken to isolate and identify the organism. serial serum igm, iga and igg antibody estimations clearly showed an acute seroconversion confirming tha ...19902384683
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
dna probe culture confirmation assay for identification of thermophilic campylobacter species.we studied the ability of a new dna probe-based assay system to correctly identify isolates of the thermophilic campylobacters campylobacter jejuni, c. coli, and c. laridis grown in vitro. we examined 424 organisms, including 214 campylobacter isolates and 210 other aerobic and anaerobic isolates. the probe assay, which uses a new homogeneous system in which all reactions take place within a single tube, demonstrated 100% accuracy, producing neither false-positive nor false-negative results. the ...19902380357
elisa for campylobacter jejuni antibodies in israeli children with diarrhea and in healthy soldiers.an elisa test was employed to detect campylobacter jejuni antibodies, using as antigen the glycine-hcl buffer extract of a local isolate. the antibody response (igg, iga and igm) was examined in a group of 32 children (age 0-12 years) with c. jejuni enteritis diagnosed by the isolation of the agent. controls were represented by two groups of children matched according to their age and sex: a) children with enteritis of unknown etiology, and b) healthy children. in addition, 66 healthy soldiers ( ...19902380033
urea protects helicobacter (campylobacter) pylori from the bactericidal effect of acid.colonization of the stomach with helicobacter (campylobacter) pylori is common in patients with duodenal ulcer disease, which is known for its high acid secretion. although the bacterium is usually isolated by culture of a gastric biopsy specimen, viable organisms may sometimes be found in the acidic gastric juice. it was postulated that urease, by generating ammonia, protected h. pylori from acid. to test this hypothesis, the ph susceptibility of h. pylori, proteus mirabilis, and the urease-neg ...19902379775
[spontaneous bacteremia caused by campylobacter jejuni]. 19902377784
campylobacter jejuni versus campylobacter coli in developing countries: how accurate are prevalence estimates? 19902373882
waterborne campylobacter jejuni epidemic in a finnish hospital for rheumatic diseases.a waterborne campylobacter jejuni outbreak in the rheumatism foundation hospital in heinola, finland, in november-december 1986 is described. 32 patients and 62 members of the staff developed gastrointestinal symptoms. c. jejuni heat-stable serotype 45 was isolated from the faeces of 32 enteritis patients and from none of the controls. no other enteropathogens were found. positive serological responses to c. jejuni acid extract antigen were detected by enzyme immunoassay in 34% of the symptomati ...19902371546
very long term diarrhoea due to campylobacter jejuni. 19902371198
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
partial purification and characterization of the enterotoxin produced by campylobacter jejuni.campylobacter jejuni enterotoxin was partially purified from culture supernatant. the purified fraction after gel filtration indicated three bands at 68, 54, and 43 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this fraction enhanced the adenylate cyclase activity of hela cell membranes by 1.5-fold over that of the control. the study with anti-cholera toxin immunoglobulin g (igg) and ganglioside affinity column chromatographies revealed that the eluent from ...19902370099
[erythema nodosum in campylobacter jejuni colitis].we report the case of a 27-years-old woman with erythema nodosum associated with ulcerative colitis due to campylobacter jejuni as diagnosed by serology, endoscopy and histology. this case indicates a hitherto rarely described etiology of erythema nodosum.19902367840
infection of adult syrian hamsters with flagellar variants of campylobacter jejuni.two variants of campylobacter jejuni in1 differing in the expression of flagella, in1 (fla+ mot+, wild type) and in1-nm (fla- mot-), were tested for their ability to establish infection in adult hamsters. animals were challenged intracecally with 2 x 10(9) to 5 x 10(10) cfu and monitored for evidence of infection. none of the challenged animals developed illness. there was a significant difference, however, in the ability of in1 to infect hamsters (35 of 43) compared with that of in1-nm (1 of 42 ...19902365459
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
serogroups of campylobacter fetus and campylobacter jejuni isolated in cases of ovine abortion.of 38 aborted ovine fetuses from 23 sheep flocks 29 c. fetus subsp. fetus and 22 c. jejuni were isolated and examined biochemically and serologically for heat-stable antigens. serologic examinations were carried out by passive haemagglutination test. in case of c. fetus subsp. fetus strains alkaline antigen extraction was used. antisera to two serogroups of c. fetus and to penner serotype reference strains 1 to 60 were produced in rabbits. abortion was caused in 18 (78.3%) flocks by c. fetus sub ...19902363324
bacterial aetiology of infantile diarrhoea in papua new guinea.bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ...19902362308
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...19902360966
epidemiology of bacterial pathogens associated with infectious diarrhea in djibouti.during a survey examining the causes of diarrhea in the east african country of djibouti, 140 bacterial pathogens were recovered from 209 diarrheal and 100 control stools. the following pathogens were isolated at comparable frequencies from both diarrheal and control stools: enteroadherent escherichia coli (eaec) (10.6 versus 13%), enterotoxigenic e. coli (etec) (11 versus 10%), enteropathogenic e. coli (epec) (7.7 versus 12%), salmonella spp. (2.9 versus 3%), and campylobacter jejuni-c. coli (3 ...19902351738
[guillain-barré syndrome following campylobacter jejuni enteritis].a 7-year-old boy nine days before onset of a guillain-barré-syndrome had had enteritis caused by campylobacter jejuni. the clinical signs were restricted to minor motor and sensory deficits in the limbs; 5 weeks after the onset of the syndrome, recovery was complete. so far, 16 cases of guillain-barré syndrome after a campylobacter enteritis have been reported in the literature.19902348889
[cryptosporidiosis in children].during january through june 1988, oocysts of cryptosporidium were identified in fecal samples of 83 (7.7%) of 1073 children who presented with acute gastroenteritis. other intestinal pathogens were less common: salmonella 4.2%, e. coli 1.9%, shigella 2.6% and campylobacter jejuni 5.0%. cryptosporidiosis (c) was more common under the age of 5 years than over (8.4% vs 1.8%, respectively, p less than 0.01). in 6 (7.2%) children with c, another stool pathogen was found. frequent signs of c were diar ...19902347532
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...19902347383
campylobacter jejuni in poultry giblets.a total of 200 poultry giblets, 50 each of chickens, ducks, squab and turkeys, were examined for the presence of campylobacter jejuni. in chicken giblets, c. jejuni was isolated from gizzards, hearts, livers and spleens with incidences of 28%, 10%, 40% and 16% respectively while 24%, 6%, 36% and 10% of duck gizzards, hearts, livers and spleens were positive for the organism, respectively. c. jejuni was detected in 6% of squab gizzards, in 10% of squab livers but failed to be detected in squab he ...19902346069
a further study of the epidemiology of campylobacter jejuni infections.epidemiological data on notifications of campylobacter jejuni infections is given for the christchurch health district area. the number of notifications rose steadily to a peak of 883 cases in 1987 (rate 261 per 100,000 population). christchurch shows consistently higher rates than the rest of new zealand. peak incidence is in the 0-4 age group; there is a high incidence amongst males and in rural areas; peak incidences in spring/summer; occupational risks have not been identified; chicken consu ...19902342690
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...19902341180
culture confirmation of campylobacter spp. by latex agglutination.a commercial latex agglutination test [meritec-campy (jcl), meridian diagnostics, cincinnati, ohio] was evaluated for identification of campylobacter jejuni, c. coli, c. laridis, and other campylobacter isolates. the test had 100% sensitivity in detecting c. jejuni and c. coli but low sensitivity with c. laridis isolates. c. upsaliensis strains reacted with the test. the test had 100% specificity for 101 non-campylobacter organisms.19902332476
protection of breast-fed infants against campylobacter diarrhea by antibodies in human milk.to investigate the role of breast-feeding in preventing diarrhea caused by campylobacter jejuni, we followed 98 mexican children prospectively for 2 years beginning at their birth. attack rates of diarrhea in children less than 6 months of age who were not fed human milk were 2.3 times greater than those in children of the same age who were fed human milk. breast-fed children remained free of diarrhea for a longer time than non-breast-fed children (p less than 0.0005). the diarrhea attack rate c ...19902329419
efficacies of erythromycin and chloramphenicol in extinguishing fecal shedding of campylobacter jejuni in dogs.oral treatment regimens of erythromycin stearate and chloramphenicol were evaluated in naturally infected laboratory colony dogs for their efficacies in extinguishing fecal shedding of campylobacter jejuni. of the 25 campylobacter-infected english foxhounds in the study, 9 were assigned to erythromycin treatment, 9 to chloramphenicol treatment, and 7 to no treatment. antimicrobials were administered for 12 days. all of the dogs that received erythromycin stearate ceased shedding c jejuni by the ...19902329075
production and characterisation of campylobacter jejuni enterotoxin in a synthetic medium and its assay in rat ileal loops.a synthetic medium for production of campylobacter jejuni enterotoxin was developed for the purposes of its purification by modifying syncase medium, replacing sucrose with glucose, and supplementing with 0.025% sodium pyruvate, 0.25% sodium metabisulphite, 0.001% ferric chloride and 0.1% l-cysteine, adjusted to ph 6.7. culture filtrates of a human diarrhoeal and a chicken isolate, grown in this medium caused fluid accumulation ranging between 0.50-0.70 ml/cm of rat ileal loop. the kinetics of t ...19902323551
enterotoxigenicity and frequency of campylobacter jejuni, c. coli and c. laridis in human and animal stool isolates from different countries.campylobacter jejuni and c. coli strains were collected during three different years from adult patients with enterocolitis in sweden (n = 372) from 49 patients in kuwait, and campylobacter strains from hens from mexico, pakistan and sweden (n = 107) and swedish pigs (n = 47). c. jejuni was the predominant species in human and hen isolates, and c. coli in pigs c. coli was significantly more common in human isolates from sweden, and more common in hen isolates from pakistan, than in hens from swe ...19902323537
campylobacter jejuni infection occurring during pregnancy.campylobacter infections occurring during pregnancy have been associated with spontaneous abortion, stillbirth, prematurity and neonatal sepsis, all ten campylobacter jejuni infections diagnosed in the approximately 24,000 pregnant women attending a 520-bed hospital between january 1984 and december 1988 were reviewed. nine women delivered healthy babies at term. in one case, campylobacter infection at 28 weeks of gestation was associated with premature labour and delivery with subsequent neonat ...19902318219
prevalence and antibiogram of campylobacter jejuni in domestic animals in rural ghana.a total of 134 samples of rectal and cloacal swabs taken from apparently healthy domestic animals that were in 43 of 76 homes located in rural ghana were examined for campylobacter jejuni. c. jejuni was isolated from 32.8% (44/134) of the animals. the highest isolation rate of 43.6% was from local domestic fowls, followed by goats (33.3%) and sheep (23%). this organism was not detected in the pigs, cats, and ducks. all 44 strains of this bacterium produced hydrogen sulphide and hydrolyzed hippur ...19902317090
campylobacter jejuni: clinical and diagnostic value of serum antibody titres.eighty patients with either bacteriologically confirmed campylobacter jejuni infection and/or an antibody titre value of at least 1:80, determined by elisa, were studied. a significant correlation was found between titre value and severity of symptoms (p = 0.015). although a correlation was noted between symptoms score and endoscopic abnormalities, this was not quite statistically significant (p = 0.053). comparison of patients with a titre of at least 1:1280 and those with lower titre values re ...19902314520
cholera-like enterotoxin in certain campylobacter jejuni strains: some observations.fourteen human isolates of campylobacter jejuni (13 pathogenic + 1 non-pathogenic) and two animal isolates were studied for the production of a toxin immunologically related to the heat-labile enterotoxin (lt) produced by a strain of escherichia coli of human (h) origin using an immuno-dot-blot assay with immunoaffinity purified antibodies against h-lt. polymyxin-b sulfate extracts of 72 hrs growth in eight of the 15 pathogenic strains were positive in immuno-dot-blot. six of these positive extr ...19902308530
isolation and antimicrobial susceptibility of campylobacter coli and campylobacter jejuni from slaughter hogs.cultural examination of cecal contents from 109 market weight hogs slaughtered in prince edward island during may-july 1988 yielded 62 isolates of campylobacter coli and seven campylobacter jejuni. a commercial latex agglutination test helped to confirm the identification of campylobacter. when tested against four drugs: erythromycin, tetracycline, kanamycin and ampicillin, 11 isolates showed multiple resistance. resistance to erythromycin was seen in 19% and 28.6% of campylobacter coli and camp ...19902308529
horizontal transmission of campylobacter jejuni amongst broiler chicks: experimental studies.horizontal transmission of campylobacter jejuni was investigated in campylobacter-free broiler chicks. one hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml c. jejuni. colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%) within 7 days], dependent on c. jejuni strain and inoculum size but independent of chick age. groups of 5-24 chicks in isolators were exposed to c. jejuni-contaminated water or co ...19902307180
adherence, enterotoxigenicity, invasiveness and serogroups in campylobacter jejuni and campylobacter coli strains from adult humans with acute enterocolitis.two hundred campylobacter jejuni and campylobacter coli strains from the same number of adult swedish patients with acute enterocolitis were tested regarding adherence to and invasiveness in hep-2 cells and for enterotoxigenicity by the cho-cell assay. the serogroup characteristics, heat-stable and heat-labile, for each strain were also investigated. eighty-four percent of the strains were classified as c. jejuni and 16 percent as c. coli. all of the strains were adherent to hep-2 cells, 39% wer ...19902302354
tropical arthritis in papua new guinea: a reactive arthritis.an idiopathic, asymmetrical oligoarthritis affecting young adults is prevalent in the tropical regions of africa, asia and melanesia. a serological study was undertaken in 23 consecutive patients with polyarthritis. each patient was assigned two paired control subjects. a standardized history, physical examination and investigations were performed. acute and convalescent sera were examined for evidence of recent infection. the presence of hla-b27 was determined. twelve (52%) of the 23 patients h ...19902300025
fecal contamination of shanty town toddlers in households with non-corralled poultry, lima, peru.we used direct observer techniques to measure the frequency with which toddler-aged children were contaminated by poultry feces in homes in a peri-urban shanty town in lima, peru. the mean number of fowl was 5.4 (sd 3.1), with 10.0 (sd 10.7) poultry defecations per 12 hours. toddlers' hand contact with poultry feces occurred a mean of 2.9 (sd 3.0) times/12 hours. a mean of 3.9 (sd 4.6) feces-to-mouth episodes per household/12 hours occurred both by direct hand-to-mouth contamination and indirect ...19902297055
production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever.four murine monoclonal antibodies (mabs) to salmonella typhi flagellin were produced. these mabs did not react with eight other enterobacterial strains tested: salmonella enteritidis, salmonella typhimurium, salmonella paratyphi a, escherichia coli, shigella flexneri, shigella sonnei, yersinia enterocolitica, and campylobacter jejuni. all four mabs cross-reacted with salmonella muenchen flagellin indicating specificity for d antigenic flagellar epitope. one mab (c4) was selected to develop a dou ...19902295860
treatment of typhoid fever and infectious diarrhoea with ciprofloxacin.ciprofloxacin and other related fluorinated 4-quinolones have microbiological and pharmacokinetic properties that suggest they could be useful agents in the management of typhoid fever and bacterial gastroenteritis. initial studies confirm that this is the case. against fully sensitive salmonella typhi ciprofloxacin is clinically as effective as chloramphenicol or co-trimoxazole. it is also effective treatment for antibiotic-resistant strains which cause epidemic and endemic infection throughout ...19902292545
[evaluation of a new medium using red-cell lysate for the isolation of campylobacter jejuni and campylobacter coli].a semiprepared medium containing horse globular lysate was evaluated for the isolation of campylobacter under two incubation atmospheres (5% o2 and candle jar). studying 19 known strains of c. jejuni, 151 human and 230 animal fecal samples. they were seeded on conventional medium (mc) and on the new medium (mlg) and incubated at 42 degrees c for 24 and 48 h. campylobacter strains grew better in 5% o2 atmosphere after 48 h of incubation. all the strains that grew or were isolated on mc, could als ...19902287716
serum antibody responses to bacterial enteropathogens in swedish travelers to south-east asia.the possibility that serological analysis may be more sensitive than bacteriological examinations of stool samples to detect enteric infections was evaluated in 80 swedish travellers to south-east asia. serum and faecal specimens were collected before, during and after their travel. serological analyses of pre-travel and any later serum specimen identified infection with enterotoxinogenic escherichia coli (etec), salmonella or campylobacter jejuni in 28% of the travellers. the seroconversion rat ...19902284576
use of tri-gas incubator for routine culture of campylobacter species from fecal specimens.we evaluated a tri-gas incubator for campylobacter isolation to be used instead of an anaerobic jar. fecal specimens were cultured in duplicate onto charcoal selective medium and incubated at 43 degrees c for 48 h in two different environments: a tri-gas incubator (forma scientific) adjusted to provide an atmosphere of 10% co2, 10% o2, and the balance n2; and evacuated anaerobic jars with a replacement gas mixture of 10% co2, 5% o2, and 85% n2. a total of 106 campylobacter jejuni and 8 campyloba ...19902280012
serodiagnosis of helicobacter pylori infection in childhood.sera from 100 children (ages, 6 to 16 years) presenting with upper gastrointestinal symptoms were examined for antibodies to helicobacter pylori by enzyme-linked immunosorbent assay (elisa) based on crude, loosely cell-associated antigens and a partially purified urease antigen preparation. all children underwent endoscopy, and 20 children were shown to have h. pylori infection by histology or direct culture. serum anti-h. pylori immunoglobulin g (igg) levels (crude antigen) were clearly raised ...19902279995
clinical and serological manifestations in patients during a waterborne epidemic due to campylobacter jejuni.a clinical and serological investigation of an epidemic due to campylobacter jejuni in a community with a population of 1026 is presented. altogether, 22 faecal samples from 27 patients were positive, with serotypes o 2 (n = 21) and o 6, 7 (n = 1) being identified. serotype o 19, 21 was isolated from drinking water which had been consumed by 89.5% households answering a questionnaire, thereby indicating an attack rate of 66.5% (i.e. 680 persons). mean duration of illness was 6.5 +/- 4.6 days. di ...19902273277
[basic and clinical studies on norfloxacin in the pediatric field].pharmacokinetic , bacteriological and clinical studies on norfloxacin (nflx), a quinolone-carboxylic acid antibacterial agent, were conducted in the pediatric field. 1. serum concentrations and urinary excretion of nflx after single dose of 2.2 approximately 5.6 mg/kg (mean 4.4 +/- 1.2 mg/kg) were determined in 13 children with ages between 6 and 11 years. the mean peak serum concentration of the drug was 0.37 +/- 0.20 micrograms/ml at 2 hours after administration. the mean half-life of the drug ...19902262968
characterization of an rrna gene-specific cdna probe: applications in bacterial identification.discontinuous dna complementary to escherichia coli 16s + 23s ribosomal rna was synthesized by random oligonucleotide priming using reverse transcriptase. cdna generated from native or denatured rrna template was labelled by incorporation of either [alpha-32p]dctp or digoxigenin-labelled dutp during synthesis, followed by template hydrolysis. the specific activity of the radiolabelled cdna was 10(7)-10(8) c.p.m. (micrograms rrna template)-1 with 60-92% incorporation after 5 h. the length of the ...19902262795
lectin typing of campylobacter isolates.isolates of campylobacter jejuni, c coli, c fetus and c laridis were tested for agglutination reactions with a panel of five lectins: arachis hypogaea, bauhinia purpurea, solanum tuberosum, triticum vulgaris and wisteria floribunda. twenty three patterns of agglutination (lectin types) were recorded among 376 isolates. patterns were consistent and reproducible. only 4.5% of isolates were untypable because of autoagglutination. some lectin types were found exclusively or predominantly in a specie ...19902262570
importance of salmonellae and campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in bangkok, thailand.the etiology of diarrhea in children less than 5 years of age in a low-income housing project in bangkok, thailand, was determined over 1 year. nontyphoidal salmonellae (13%), campylobacter jejuni (12%), rotavirus (12%), enterotoxigenic escherichia coli (7%), shigellae (6%), e. coli that hybridized with the enteropathogenic e. coli adherence factor probe (3%), and enteroinvasive e. coli (1%) were identified in 345 episodes of diarrhea in children less than 5 years of age. salmonellae were identi ...19902254427
[the adhesion of clinical isolates of campylobacter jejuni to intestinal epithelial cells in vitro].the adhesive activity of c. jejuni isolated from feces of children with campylobacter infection was studied with the use of a newly developed model. 47 clinical isolates were analyzed; of these, 91% were found to be enteroadhesive to a variable degree. as the result of in vitro studies, campylobacter were found to have much greater tropism to colonic cells and epithelial cells of peyer's patches in comparison with the epithelial cells of the small intestine. the correlation between the degree of ...19902251887
hepatotoxic activity of campylobacter jejuni.hepatotoxic factor(s) were isolated from whole-cell lysates of campylobacter jejuni gifu 8734 and purified by chromatography. a single intravenous injection of 10 micrograms of this factor reproducibly produced hepatitis in mice, as determined by histology and liver function tests. the hepatic lesions were very similar to those evoked by c. jejuni infection. tissue-culture studies with mouse hepatocytes demonstrated that low concentrations of the factor caused release of hepatic enzymes into the ...19902250287
reproductive failure in mink and ferrets after intravenous or oral inoculation of campylobacter jejuni.four pregnant mink and seven pregnant ferrets, including five with previous exposure and specific antibody, were injected intravenously with 10(8)-10(10) colony-forming units of campylobacter jejuni. all 11 pregnancies failed 1-16 days after infection, with results ranging from fetal resorption to expulsion of dead or premature living kits. in every case, uterine contents (placenta, uterine fluid and/or kits) were culture-positive for c. jejuni. three pregnant mink and nine pregnant ferrets, inc ...19902249178
acute axonal polyneuropathy associated with anti-gm1 antibodies following campylobacter enteritis.we report 2 patients with guillain-barré syndrome (gbs) following campylobacter jejuni enteritis. electrophysiologic studies indicated that the predominant process was axonal degeneration of motor nerves, and clinical recovery was poor. serum testing by thin-layer chromatography and enzyme-linked immunosorbent assay revealed that the sera from both patients contained high titers of igg antibody against gm1 ganglioside. these cases may represent a subgroup of gbs as acute axonal polyneuropathy fo ...19902247243
study of haemolytic activity of some campylobacter spp. on blood agar plates.a total of 152 strains of campylobacter jejuni, c. coli, c. laridis and c. fetus subsp. fetus were tested for haemolysis on blood agar plates. distinct haemolysis was detected in 92.3% (96/104) of strains of c. jejuni and 21.7% (5/23) of strains of c. coli on sheep blood heart infusion agar after incubation for 4 d microaerobically at 42 degrees c. haemolysis was also detected on horse blood heart infusion agar. haemolysis was not detected at 37 degrees c except with one of 50 strains of c. jeju ...19902246143
size and physical map of the campylobacter jejuni chromosome.the chromosome of campylobacter jejuni is circular and approximately 1700 kb in circumference. the size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda dna concatamers and yeast chromosomes to calibrate the size of the fragments. in view of the low (32-35%) g + c content of the campylobacter genome, enzymes that recognizes gc-rich sequences were used. of the enzymes tested bsshii (g/c(g)cgc), ncii (cc/cgcg) and sali (g/tcgac) ...19902243769
[the pathogenicity of campylobacter jejuni as a monoinfection and as a mixed infection with escherichia coli 078:k80 in broilers].two week old broilers (n = 61) with a monoinfection with campylobacter jejuni (0.5 ml of suspension containing 10(5) cfu/ml per os) showed reduced increase in weight during week 3 after infection compared to the control group. an other group of chickens (n = 31) was additionally infected with a suspension of escherichia (e.) coli o78:k80 via drinking water from day 4 to 6 after the primary infection. this mixed infection provoked clinical signs of a disease and reduced increase in weight during ...19902241888
influence of host lineage on cecal colonization by campylobacter jejuni in chickens.the resistance to cecal colonization by campylobacter jejuni was assessed by challenging three crossbred stocks of commercially available broiler chickens. these three stocks, designated a, b, and c, were related as follows: offspring from four pedigreed grandparent flocks were used as progenitors. stock b was derived by cross-breeding grandparent 1 with grandparent 3. stocks a and c were crossbreeds from grandparents 1 and 2 and grandparents 3 and 4, respectively. campylobacter jejuni were gava ...19902241687
influence of antibody treatment of campylobacter jejuni on the dose required to colonize chicks.this study was designed to clarify the role of antibodies in controlling chicken colonization by campylobacter jejuni. cecal colonization by c. jejuni was compared after the organism was exposed either to phosphate-buffered saline, normal rabbit serum, rabbit hyperimmune anti-c. jejuni serum, or anti-c. jejuni antibodies extracted from chicken bile. antibodies from chicken bile were extracted by affinity absorption against outer-membrane proteins from the challenge organism. sera were heated 1 h ...19902241686
influence of campylobacter jejuni cecal colonization on immunoglobulin response in chickens.the immunoglobulin response of chickens to colonization by campylobacter jejuni isolates b-540 and clin-1 was monitored. chicken humoral igg and biliary secretory iga (siga) responses were assessed by enzyme-linked immunosorbent assay (elisa). samples were taken from 128 c. jejuni-colonized chickens and 104 uncolonized chickens housed in a controlled environment. an indirect elisa was performed using the homologous isolate of c. jejuni as the capture antigen and was developed with the specific g ...19902241685
some early observations on hiv infection in children at king edward viii hospital, durban.nine black children aged between 3 months and 30 months of age, with human immunodeficiency virus type i (hiv-i) infection are described to draw the attention of health professionals in southern africa to special clinical characteristics useful for recognising this problem, which has many shared features with common diseases of infancy and childhood in the third world. the main presenting complaints were chronic cough and persistent diarrhoea and vomiting. these children frequently had diarrhoea ...19902237685
[identification of campylobacter jejuni by using a biotinylated probe].a dna biotinylated probe for the rapid identification of campylobacter jejuni in culture (enzo biochem, new york) has been evaluated. the hybridized biotinylated dna probe is detected by its interaction with streptavidin linked to horseradish peroxidase. sixteen strains of c. jejuni, including type strain. 24 strains of other campylobacter and helicobacter species, and 59 strains of other general have been studied. the probe was highly sensitive (100%) and specific (100% inside the genera campyl ...19902235085
[serology and culture diagnosis of campylobacter jejuni infections].campylobacter jejuni is known today as one of the most common pathogens in acute infectious enteritis. in 77 patients serological testing by complement fixation as well as stool cultures were performed. a campylobacter jejuni infection was identified in 53% by culture and in 64% by serology, only in 17% the diagnosis was made with both methods. we conclude, that because of these data stool culture and serological testing should be used simultaneously. the complement fixation technique shows an i ...19902233597
[the serotype distribution of campylobacter jejuni strains among gastroenteritis in hospitals over 7 year period in tokyo].campylobacter jejuni strains isolated from gastroenteritis at 4 general hospitals of tokyo metropolitan during the period from 1981 to 1987 were serotype according to the slide agglutination test (tck system) developed by the tokyo metropolitan research laboratory of public health. two thousand four hundred seventy-nine strains isolated from sporadic cases among infants and children, 1,962 (78.5%) were typed by 33 typing sera numbered tck 1 through tck 33 and leaving 537 strains (21.5%) untypabl ...19902230367
evaluation of cytotoxic activity in fecal filtrates from patients with campylobacter jejuni or campylobacter coli enteritis.we sought to determine the prevalence of cytotoxic activity in fecal filtrates from persons with c. jejuni or c. coli enteritis. stool specimens were collected from 20 persons with c. jejuni or c. coli enteritis, 20 persons with acute diarrheal illnesses of other causes, and 9 healthy, asymptomatic persons. fecal filtrates were then incubated with chinese hamster ovary (cho) or hela cells. the fecal filtrate from 1 of the 20 (5%) persons with campylobacter enteritis was cytotoxic for hela cells ...19902227365
[etiology of diarrheal diseases in immunocompetent and hiv-positive patients].in 1986 and 1987 the stool samples of 206 patients of the medical outpatient clinic basel were examined prospectively for bacteria, protozoa and worms. clinical data of the patients were recorded by questionnaire. the patient group comprised 63 immunocompetent patients as well as 23 hiv-infected patients, all with symptoms of acute enteritis. the control group consisted of 120 healthy persons. pathogenic organisms were found in the stools of 17.5% of the immunocompetent patients with enteritis. ...19902218447
salmonella-reactive synovial fluid t-cell clones in a patient with post-infectious salmonella arthritis.from a patient with reactive arthritis following salmonella typhimurium enteritis, synovial fluid t-lymphocytes were cloned and expanded in vitro. seven out of 74 clones showed a marked proliferative response to antigens of heat-killed salmonella typhimurium with autologous t-cell-depleted peripheral blood mononuclear cells as antigen-presenting cells. the salmonella-reactive clones were of the cd4+ phenotype, antigen-induced proliferation could be inhibited by a monoclonal antibody to hla class ...19902218431
structural and functional analysis of two campylobacter jejuni flagellin genes.the purpose of this study was to characterize the flagellin gene of campylobacter jejuni and to study the structure of this protein and the regulation of its synthesis. a part of the flagellin gene of c. jejuni strain 81116 was recently cloned by us. this dna fragment was used as a probe to isolate the other homologous flagellin sequences from genomic libraries. the flagellin nucleotide sequence was determined from overlapping clones. two copies of the flagellin gene were identified: genes fla a ...19902211662
identification of catalase negative/weak campylobacter jejuni from human blood and faecal cultures by numerical analysis of electrophoretic protein patterns.twenty-eight isolates of catalase-negative/weak (cnw) thermophilic campylobacters from human blood and faecal cultures were characterized by one-dimensional (1-d) high-resolution sds-page of cellular proteins. a further 11 campylobacter strains were included for reference purposes. partial protein patterns were used as the basis for numerical analysis, which showed that all of the hippurate-positive strains had a high similarity to c. jejuni. two subclusters were formed within c. jejuni correspo ...19902210345
an outbreak of campylobacter enteritis associated with a community water supply.in june 1987, an outbreak of acute enteritis occurred over a period of 2 weeks in a small rural community where the potable water supply is unfiltrated and unchlorinated. campylobacter jejuni was isolated from 6 patients. a case-control study performed in a local fiberglass shop where workers drink a lot of water, showed an association between the occurrence of enteritis and the consumption of 10 or more 8-ounce glasses of water per day (odds ratio = 6, p = 0.04). the attack rate of enteritis wa ...19902207948
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
acute gastroenteritis due to double infection with enteropathogenic escherichia coli or salmonella and another bacterial pathogen.two enteric bacterial pathogens were concomitantly isolated from the feces of 18 infants less than 6 months of age admitted to the assaf harofeh medical center over a 7-year period. in all but two patients stool cultures grew enteropathogenic escherichia coli, six of serogroup 0119. the other organisms cultured were shigella, salmonella, campylobacter jejuni and aeromonas hydrophila. the usual clinical presentation was diarrhea, dehydration and vomiting of acute onset, and low grade fever. patie ...19902199408
vacation diarrhea. how should it be managed?traveler's diarrhea in north america is most often a self-limited disease that requires little medical intervention unless the course is prolonged or especially severe. diagnosis is almost always made by a stool examination or culture. the most common causative organisms are campylobacter jejuni and giardia lamblia. enterotoxigenic escherichia coli is a less frequent cause. patients should be advised regarding the need for fluid replacement. antibiotic therapy should be considered only in rare c ...19902195482
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
[evaluation of effectiveness of rokitamycin dry syrup in acute enteritis in pediatrics. a comparative study on rokitamycin and fosfomycin dry syrups].because campylobacter jejuni is most frequently identified as a causative organism of bacterial enteritis in pediatrics, a study was done to evaluate the clinical efficacy against campylobacter enteritis and the safety of a macrolide antibiotic, rokitamycin (rkm). in case of acute enteritis, rkm was used in a form of dry syrup at a dose level of approximately 30 mg (in potency)/kg body weight and its efficacy and safety were compared to those of fosfomycin (fom) dry syrup which is currently in u ...19902194055
[surgical treatment of infectious aneurysm of the popliteal artery. description of a case caused by campylobacter jejuni and a review of the literature].the authors describe a case of mycotic aneurysm of the popliteal artery secondary to campylobacter jejuni-derived infectious endocarditis treated by excision and in situ femoro tibial venous bypass, and take the opportunity to review 14 literature cases of infectious aneurysm of popliteal artery. this diagnosis is most frequently evoked by the development in an infectious setting of a throbbing inflammatory mass in the popliteal fossa. management is aimed at controlling the infection and insurin ...19902193936
to the problem of campylobacter jejuni detectability in water.in a series of model experiments two isolation procedures for the detection of water-borne campylobacter jejuni were compared: a standard culture in thioglycolate broth enriched with 7% defibrinated sheep blood and supplement c and a modified membrane filtration method in which the filter (porosity 0.45 microns) plated on campylobacter agar surface was removed after the first 24 hours of incubation and the plate further incubated for 48 hours. the recovery rates by the thioglycolate broth method ...19902191035
factors responsible for the introduction and spread of campylobacter jejuni infection in commercial poultry production.campylobacter jejuni and related thermophilic campylobacters were not found in a hatchery or in chicks aged less than seven days. however, an increasing proportion of chicks aged two weeks and older shed campylobacters in their droppings. it was shown that a likely source of c jejuni for young chicks was the environment in the immediate vicinity of the rearing houses, and that infection could readily be introduced on the footwear and clothing of farm staff. thermophilic campylobacters were found ...19902188414
molecular studies on the mechanism of tetracycline resistance mediated by tet(o).the mechanism of resistance to tetracycline in escherichia coli mediated by the campylobacter jejuni-derived resistance determinant tet(o) was investigated. the cloned tet(o) protein had no detectable effect on the intracellular accumulation of tetracycline. the presence of tet(o) markedly diminished the inhibitory effect of tetracycline on protein synthesis both in vivo and in vitro. ribosomes prepared from tetracycline-resistant and susceptible e. coli cells bound almost identical amounts of r ...19902183711
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
catalase activity in campylobacter jejuni: comparison of a wild-type strain with an aerotolerant variant.a comparison of campylobacter jejuni vpi strain h840 (atcc 29428), which can grow at o2 levels up to 15%, with variant strain mc711-01 (which can grow at o2 levels up to 21-26%) indicated that the specific activity of catalase in crude cell extracts was higher in the variant by a factor of 1.6 to 2.5, depending on cultural conditions. smaller differences occurred with superoxide dismutase activity, while peroxidase activities were invariably lower in the variant strain. the variant strain was mu ...19902168804
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni ua580.to determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
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