Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| development of listeriosis during vancomycin therapy in a neutropenic patient. | vancomycin and aminoglycosides are widely used for fever in neutropenic patients. this combination of antibiotics has also been proposed for the treatment of listeria monocytogenes bacteremia. we report a case of fatal listeriosis in a 33-year-old man during acute lymphoblastic leukemia induction therapy in a total protective environment despite him having been on vancomycin for 8 days for treatment of staphylococcus bacteremia. vancomycin and aminoglycoside cannot be proposed for the treatment ... | 1996 | 8893409 |
| listeriosis in 225 non-pregnant patients in 1992: clinical aspects and outcome in relation to predisposing conditions. | clinical information was collected on 225 cases of listeria monocytogenes (lm) infection not associated with pregnancy occurring in france in 1992. infections affected primarily men (62%) and persons over 65 years of age. of the cases found, 81% occurred in persons with some underlying condition: 34% involved patients with severe immunosuppression; 37% were on dialysis or had diabetes mellitus, alcoholism, hepatic failure or malignancies without immunosuppressive therapy; and 10% had other under ... | 1996 | 8893400 |
| sodium-driven, osmotically activated glycine betaine transport in listeria monocytogenes membrane vesicles. | transport of the osmoprotectant and cryoprotectant glycine betaine was investigated in membrane vesicles of listeria monocytogenes. uptake-driving transmembrane potentials ranging from 111 to 122 mv within the ph range of 5.5 to 7.5 could be generated by the electron donor system ascorbate-phenazine methosulfate but not by the electron donor system ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine. transport was dependent on both high concentrations of sodium ion and the presence of a hypertoni ... | 1996 | 8892806 |
| the listerial exotoxins listeriolysin and phosphatidylinositol-specific phospholipase c synergize to elicit endothelial cell phosphoinositide metabolism. | exotoxins such as listeriolysin (llo) and phosphatidylinositol-specific phospholipase c (pica) have been implicated in listerial infection and sepsis. employing different listeria strains, mutated in individually known virulence genes, we examined exotoxin-related induction of endothelial cell signaling. listeria monocytogenes was a potent inductor of phosphatidylinositol (ptdins) metabolism in huvec. this effect was completely absent in a llo-negative strain. using a recombinant listeria innocu ... | 1996 | 8892639 |
| is amoxicillin-cotrimoxazole the most appropriate antibiotic regimen for listeria meningoencephalitis? review of 22 cases and the literature. | from june 1983 to january 1994, 22 adult patients with severe listeria monocytogenes meningoencephalitis were observed in our intensive care unit. listeria monocytogenes was obtained in culture in cerebrospinal fluid or blood for every patient. seven patients were treated with the combination ampicillin-aminoglycoside (group a) and 15 patients with the combination ampicillin (or amoxicillin)-cotrimoxazole (group a + c). risk factors and gravity scores were similar in both groups. failure of the ... | 1996 | 8889993 |
| acid tolerance in listeria monocytogenes: the adaptive acid tolerance response (atr) and growth-phase-dependent acid resistance. | listeria monocytogenes acquired increased acid tolerance during exponential growth upon exposure to sublethal acid stress, a response designated the acid tolerance response (atr). maximal acid resistance was seen when the organism was exposed to ph 5.0 for 1 h prior to challenge at ph 3.0, although intermediate levels of protection were afforded by exposure to ph values ranging from 4.0 to 6.0. a 60 min adaptive period was required for the development of maximal acid tolerance; during this perio ... | 1996 | 8885415 |
| identification of a clpc atpase required for stress tolerance and in vivo survival of listeria monocytogenes. | we identified a new chromosomal locus involved in the virulence of the facultative intracellular pathogen listeria monocytogenes. this locus displays the same genetic organization as that of the clpc/mecb locus of bacillus subtilis. it contains a thermoregulated operon of four genes, whose transcription is upregulated at 42 degrees c. the last gene of this operon is clpc, which encodes a protein of 826 amino acid residues, identified as a clpc atpase, sharing a strong peptide sequence identity ( ... | 1996 | 8885268 |
| perinatal infection with listeria monocytogenes. | listeria monocytogenes has been increasingly recognized as a cause of intrauterine sepsis with associated perinatal wastage. the condition is mostly acquired through dietary intake and appropriate advice should be given to all pregnant women. the most common presentations in pregnancy include premature labour, an influenza-like illness and reduced fetal movements. in this report, we present a series of 24 cases of perinatal listeria infection presenting to either our obstetric or neonatal units ... | 1996 | 8883752 |
| the loss of contact inhibition and anchorage-dependent growth are key steps in the acquisition of listeria monocytogenes susceptibility phenotype by non-phagocytic cells. | we have previously demonstrated that intestinal and kidney finite cell lines were resistant to l monocytogenes invasion (ie allowed low bacterial entry and no intracellular multiplication) in contrast to the continuous cell lines which were susceptible to listeria invasion (ie allowed high bacterial entry and intracellular multiplication) (velge et al (1994a) med microbial immunol 183, 145). the aim of this study was to discover whether epigenetic or genetic cellular modifications could convert ... | 1995 | 8882519 |
| cd4+ cytolytic effectors are inefficient in the clearance of listeria monocytogenes. | cytotoxic t lymphocytes (ctl) recognize and lyse target cells through the interaction of the t-cell receptor complex with the class i or class ii major histocompatibility complex (mhc). the production of class i-restricted ctl has been shown to be critical to the elimination of specific pathogens including listeria monocytogenes. however, the function of class ii-restricted ctl in the clearance of intracellular pathogens is poorly understood. h-2b beta 2-microglobulin-deficient mice (beta 2m-/-) ... | 1996 | 8881755 |
| effect of combined lysozyme and lipase treatment on the survival of listeria monocytogenes. | the effectiveness of polyphosphates or lipases to increase the lytic activity of lysozyme was evaluated both on listeria monocytogenes suspended in buffer and on growing cultures incubated at different temperatures. at 5 degrees c and 37 degrees c polyphosphates combined with lysozyme did not result in the decrease of the number of non-growing l. monocytogenes cells. at the same incubation conditions, the addition of lipase to lysozyme significantly enhanced the bactericidal activity of lysozyme ... | 1996 | 8880343 |
| listeria monocytogenes in bovine mastitis. possible implication for human health. | during the 23-year period 1972 through 1994 quarter milk samples from 1,132,958 cows originating from 36199 herds were examined for the presence of listeria monocytogenes. through the period the reference population amounted to 12,742,600 cow years and 401,682 herd years. the percentage of cows infected with l. monocytogenes varied from 0.01 to 0.1% (mean 0.04%) and of herds with an infected cow from 0.2 to 4.2% (mean 1.2%) through the period, showing a low but constant level of infection. a com ... | 1996 | 8880340 |
| effects of carbon dioxide on the fate of listeria monocytogenes, of aerobic bacteria and on the development of spoilage in minimally processed fresh endive. | minimally processed fresh broad-leaved endive (cichorium endivia l.) were stored at 3 and 10 degrees c in modified atmospheres containing air, 10% co2/10% o2, 30% co2/10% o2, and 50% co2/10% o2. the effects of these modified atmospheres on the fate of both aerobic bacteria and three strains of listeria monocytogenes, was investigated. increases in co2 concentrations significantly reduced the growth of the aerobic microflora. the best preservation of the visual quality occurred on endive leaves s ... | 1996 | 8880336 |
| predictive modeling of the growth of listeria monocytogenes in co2 environments. | the effects of ph (5.5, 6.5), temperature (4, 7 and 10 degrees c) and carbon dioxide (10, 30, 50, 70 and 90%) on the growth and/or survival of a five strain mixture of listeria monocytogenes were examined in brain heart infusion broth. all three variables had a major influence on the growth characteristics of the organism. as expected, both the lag time and generation time increased as the co2 level increased, and as ph and temperature decreased. growth over a 30-day period was observed at all p ... | 1996 | 8880334 |
| predictive models of the effect of temperature, ph and acetic and lactic acids on the growth of listeria monocytogenes. | the combined effect of temperature (1-20 degrees c), ph (4.5-7.2) and acetic acid (0-10,000 mg/l; model 1) or lactic acid (0-20,000 mg/l; model 2) on growth of listeria monocytogenes in laboratory media was studied. growth curves at various combinations of temperature, ph and acid concentration were fitted by the model of baranyi and roberts (1994), and specific growth rates derived from the curve fit were modelled. predictions of growth from the models were compared with data in the literature, ... | 1996 | 8880329 |
| the sub-typing of listeria monocytogenes isolates from food, environments surrounding food manufacturing sites, and clinical samples in new zealand using multilocus enzyme electrophoresis. | listeria monocytogenes strains isolated from food (cheese, meat and fish), environments surrounding food manufacturing sites, and clinical samples from throughout new zealand were analyzed by multilocus enzyme electrophoresis. one hundred and twenty four strains were divided into 56 electrophoretic types, containing primarily clinical isolates and environmental isolates. | 1996 | 8880322 |
| incidence of listeria spp. in tropical fish. | the incidence of listeria spp. in tropical fish and shellfish was studied. the isolation protocol included a pre-enrichment, followed by two selective enrichment steps and plating on three selective agars. listeria monocytogenes could be detected in 17.2% of finfish and 12.1% of shellfish. l. innocua was the most common species encountered. in 6.9% finfish and 5.6% shellfish, both l. monocytogenes and l. innocua were detected. polymerase chain reaction (pcr)-based amplification of internal fragm ... | 1996 | 8880320 |
| typing of listeria monocytogenes by monocin and phage receptors. | one hundred strains of listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. the monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. the results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and rapd (random amplification of polymorphic dna). the monocin/phage types were comparab ... | 1996 | 8880312 |
| pathways of listeria monocytogenes contamination in the meat processing industry. | one hundred and thirty-three isolates of listeria monocytogenes from deboned fresh meat, production environment, cold cuts from five meat processing plants and from one plant producing cured dried sausages, were characterized using multilocus enzyme electrophoresis. on the basis of electrophoretically demonstrable allelic variation at 21 enzyme loci, 21 electrophoretic types (ets) were distinguished. analysis of the genetic relationships among the 21 ets revealed two distinct clusters: cluster a ... | 1996 | 8880305 |
| listeriolysin o production and pathogenicity of non-growing listeria monocytogenes stored at refrigeration temperature. | three haemolytic, pathogenic strains of listeria monocytogenes (a reference strain nctc 7973, a food-derived strain l70 and a human strain l94) and a control strain of listeria innocua l27 were held in phosphate-buffered saline (pbs) of ph 7.0 or 5.5 at 4 degrees c for 4 weeks. the number of viable cells did not change significantly during this storage (the cells were non-growing). titers of listeria listeriolysin o (llo) activity against washed human erythrocytes and the pathogenicity of non-gr ... | 1996 | 8880303 |
| changes in microbial populations on fresh cut spinach. | the microbial populations found on fresh-cut spinach leaves that were stored in gas permeable bags at 10 degrees c for 12 days were examined and identified. the microorganisms consisted of mesophilic aerobic bacteria, psychrotrophic bacteria, pseudomonadaceae, enterobacteriaceae, micrococcaceae, lactic acid bacteria and yeasts. populations of mesophiles, psychrotrophs, pseudomonadaceae and enterobacteriaceae increased sharply during the storage period. the initial populations were 10(7), 10(6), ... | 1996 | 8880301 |
| immune and inflammatory responses in tnf alpha-deficient mice: a critical requirement for tnf alpha in the formation of primary b cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. | to investigate the role of tnf alpha in the development of in vivo immune response we have generated tnf alpha-deficient mice by gene targeting. homozygous mutant mice are viable and fertile, develop lymph nodes and peyer's patches and show no apparent phenotypic abnormalities, indicating that tnf alpha is not required for normal mouse development. in the absence of tnf alpha mice readily succumb to l. monocytogenes infections and show reduced contact hypersensitivity responses. furthermore, tnf ... | 1996 | 8879212 |
| pulsed light and pulsed electric field for foods and eggs. | two new technologies for use in the food industry are described. the first method discussed uses intense pulse of light. this pulsed light (purebright) process uses short duration flashes of broad spectrum "white" light to kill all exposed microorganisms, including vegetative bacteria, microbial and fungal spores, viruses, and protozoan oocysts. each pulse, or flash, of light lasts only a few hundred millionths of a second (i.e., a few hundred microseconds). the intensity of each flash of light ... | 1996 | 8878274 |
| thermal and biological treatments to control psychrotrophic pathogens. | over the past decade, advances in egg processing technologies have permitted commercial production of ultrapasteurized liquid whole egg (lwe) products with a shelf-life of greater than 10 wk at 4 c. the inactivation and control of psychrotrophic pathogens such as listeria monocytogenes and aeromonas hydrophila in extended shelf-life lwe and conventionally pasteurized egg products is an ongoing food safety concern. this manuscript reports on the common features of these two psychrotrophic pathoge ... | 1996 | 8878273 |
| a new prfa-regulated gene of listeria monocytogenes encoding a small, secreted protein which belongs to the family of internalins. | a mutant of listeria monocytogenes egd was constructed that carries an extended deletion removing the entire prfa-regulated gene cluster from plca to plcb and a second deletion inactivating the inla gene. upon supplementation of this mutant with multiple gene copies of prfa, a protein of 30 kda was detected in the supernatant of the mutant strain. the gene encoding this protein was obtained by direct and inverse polymerase chain reaction using oligonucleotide primers that were deduced from parti ... | 1996 | 8878044 |
| in-vitro activity of ramoplanin (a novel lipoglycopeptide), vancomycin, and teicoplanin against gram-positive clinical isolates from cancer patients. | the in-vitro activities of ramoplanin, vancomycin, and teicoplanin against gram-positive organisms isolated from cancer patients were determined. ramoplanin was the most active agent tested inhibiting all isolates at a concentration of < or = 0.5 mg/l. although all isolates were also susceptible to vancomycin and teicoplanin, their activities were surpassed by that of ramoplanin. the activity of teicoplanin was moderately better than that of vancomycin against bacillus cereus, enterococcus faeci ... | 1996 | 8877541 |
| induction of nitric oxide synthase in bovine mononuclear phagocytes is differentiation stage-dependent. | bovine monocytes and monocyte-derived macrophages (mdm) activated by various means were assessed for induction of inducible nitric oxide synthase (inos), using the griess assay, northern blotting and reverse transcription/polymerase chain reaction (rt-pcr). interferon-gamma (ifn-gamma) induced little, if any, inos expression and no production in mdm, although these cells responded to ifn-gamma in other regards. in contrasts, mdm produced copious amounts of no when stimulated with lps or salmonel ... | 1996 | 8877411 |
| major histocompatibility class i presentation of soluble antigen facilitated by mycobacterium tuberculosis infection. | cell-mediated immune responses are essential for protection against many intracellular pathogens. for mycobacterium tuberculosis (mtb), protection requires the activity of t cells that recognize antigens presented in the context of both major histocompatibility complex (mhc) class ii and i molecules. since mhc class i presentation generally requires antigen to be localized to the cytoplasmic compartment of antigen-presenting cells, it remains unclear how pathogens that reside primarily within en ... | 1996 | 8876215 |
| h2-m3 restricted presentation of listeria monocytogenes antigens. | 1996 | 8872487 | |
| divergicin 750, a novel bacteriocin produced by carnobacterium divergens 750. | divergicin 750, a bacteriocin produced by carnobacterium divergens 750, preferentially inhibited the growth of strains of carnobacterium and enterococcus. selected strains of listeria monocytogenes and clostridium perfringens were also inhibited. the bacteriocin was purified to homogeneity by ammonium sulfate precipitation and sequential s-sepharose, hydrophobic interaction and reversed phase chromatography. the complete amino acid sequence was determined by edman degradation. the peptide consis ... | 1996 | 8869500 |
| an essential role for acta in acid tolerance of rhizobium meliloti. | the acta gene, which is disrupted by tn5 in the acid-sensitive mutant of rhizobium meliloti tg2-6, was cloned and sequenced. it encodes a protein of 541 amino acids with a calculated molecular mass of 57,963 da and an estimated pl of 9.0. the acta protein sequence has 30% identity, and much higher similarity (69%), with the cute protein of escherichia coli. like the cute mutant of e. coli, tg2-6 is sensitive to copper. the reconstructed wild-type acta gene complemented the low ph- and copper-sen ... | 1996 | 8868435 |
| liver abscesses due to listeria monocytogenes. | involvement of the liver in cases of listeria monocytogenes is uncommon. we report a case of hepatitis due to l. monocytogenes, with a pathological finding of multiple abscesses. blood cultures yielded l. monocytogenes. the patient died a few days after admission. | 1996 | 8868081 |
| expression of an adenovirally encoded lymphotoxin-beta inhibitor prevents clearance of listeria monocytogenes in mice. | the lymphotoxin (lt)-beta heterotrimer was recently identified as a molecule containing lt-alpha subunits, tethered to the cell through non-covalent association with an integral plasma membrane protein, derived from the lt-beta gene. since knockout mutations of the lt-alpha gene yield animals that lack lymph nodes, whereas animals lacking either or both of the receptors for tumor necrosis factor (tnf) and lt-alpha homotrimers have normal lymph nodes, it has been inferred that the association bet ... | 1995 | 8867668 |
| the prevalence of listeria monocytogenes in the environment of dairy farms. | the prevalence of listeria monocytogenes in the environment of dairy farms was surveyed from december 1993 to june 1994 in one city of hokkaido. l. monocytogenes was isolated from 3 out of 5 farms investigated. serovar 4b organism was isolated from the brain stem of a cow from one farm which was clinically diagnosed as having listeriosis. the same serovar of l. monocytogenes was also isolated from the rectal contents of a healthy cow, straw on the floor, straw in the barn, and silage scattered a ... | 1996 | 8867607 |
| internalin must be on the bacterial surface to mediate entry of listeria monocytogenes into epithelial cells. | entry of listeria monocytogenes into cultured epithelial cells requires production of internalin, a protein with features characteristic of some gram-positive bacterial surface proteins, in particular an lpxtg motif preceding a hydrophobic sequence and a few basic residues at its c-terminal end. by immunofluorescence and immunogold labelling, we show that in wild-type l. monocytogenes, internalin is present on the cell surface and has a polarized distribution similar to that of acta, another sur ... | 1996 | 8866480 |
| a role for phosphoinositide 3-kinase in bacterial invasion. | listeria monocytogenes is a bacterial pathogen that invades cultured nonphagocytic cells. inhibitors and a dominant negative mutation were used to demonstrate that efficient entry requires the phosphoinositide (pi) 3-kinase p85alpha-p110. infection with l. monocytogenes caused rapid increases in cellular amounts of pi(3, 4)p2 and pi(3,4,5)p3, indicating that invading bacteria stimulated pi 3-kinase activity. this stimulation required the bacterial protein inlb, host cell tyrosine phosphorylation ... | 1996 | 8864117 |
| listeria spinal cord abscess--clinical and mri findings. | intramedullary spinal cord abscess due to listeria monocytogenes is an uncommon condition usually affecting immunocompromised patients. | 1996 | 8862846 |
| subtyping listeria monocytogenes isolates genetically related to the swiss epidemic clone. | macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (et1) clone of listeria monocytogenes. for this purpose, a total of 144 isolates from switzerland shown by multilocus enzyme electrophoresis to belong to the et1 were examined. these isolates were subtyped by macrorestriction analysis using the enzymes apai and smai and field inversion gel electrophoresis. among these 144 isolates, 45 wer ... | 1996 | 8862575 |
| mena, a relative of vasp and drosophila enabled, is implicated in the control of microfilament dynamics. | drosophila enabled is required for proper formation of axonal structures and is genetically implicated in signaling pathways mediated by drosophila abi. we have identified two murine proteins, mena and evl, that are highly related to enabled as well as vasp (vasodilator-stimulated phosphoprotein). a conserved domain targets mena to localized proteins containing a specific proline-rich motif. the association of mena with the surface of the intracellular pathogen listeria monocytogenes and the g-a ... | 1996 | 8861907 |
| the use of pcr ribotyping for typing strains of listeria spp. | the potential of pcr ribotyping for discriminating between and within various species of listeria, as well as strains of listeria monocytogenes was examined. in total, 49 strains of listeria monocytogenes and 12 isolates of listeria spp. were analyzed. the genomic dna isolated from these strains was subjected to pcr amplification in the regions between 16s and 5s rrna. amplifications were performed with both low and high concentrations of taq polymerase. length polymorphisms in the amplified dna ... | 1995 | 8861851 |
| inhibition of the multiplication of listeria monocytogenes in a murine hepatocyte cell line (atcc tib73) by ifn-gamma and tnf-alpha. | the effects of ifn-gamma and tnf-alpha on intracellular multiplication of listeria monocytogenes in a murine enbryonic hepatocyte cell line (atcc tib 73) were investigated. neither ifn-gamma nor tnf-alpha alone (10, 25, or 100 ng/ml) significantly inhibited intracellular multiplication of l. monocytogenes. in contrast, addition of both ifn-gamma and tnf-alpha (10, 25, or 100 ng/ml) significantly inhibited intracellular listerial multiplication. the anti-listerial effects of ifn-gamma and tnf-alp ... | 1996 | 8861394 |
| apoptosis of mouse dendritic cells is triggered by listeriolysin, the major virulence determinant of listeria monocytogenes. | infection of a murine-spleen dendritic cell line by listeria monocytogenes was found to induce cell death through apoptosis. to characterize the bacterial product(s) involved in induction of apoptosis, dendritic cells were infected with the l. monocytogenes egd strain and several isogenic mutants deficient in the production of individual listerial virulence factors. the ability to induce cellular apoptosis was retained by all mutants tested, except the prfa and delta hly mutants, both of which a ... | 1996 | 8861210 |
| live tularemia vaccine confers protection against lethal legionella and listeria infections in experimental animals. | the efficacy of a live francisella tularensis vaccine strain to cause nonspecific immunity toward experimental legionellosis and listeriosis was studied. immunisation with tularemia vaccine protected over 80% and 17% of experimental animals against subsequent lethal challenge with legionella pneumophila and listeria monocytogenes, respectively. the protection was maximal during the first month following immunisation and declined thereafter. in order to delineate the immunostimulatory moieties of ... | 1996 | 8861031 |
| health risk assessment of listeria monocytogenes in canada. | in this review, the major steps used in the formulation of a health risk assessment for listeria monocytogenes in foods are discussed. data is given on the numbers of human listeriosis cases reported in canada along with the current canadian regulatory policy on l. monocytogenes. four major steps in the health risk assessment of this organism in foods, namely, hazard identification, hazard characterization, exposure assessment and risk characterization, were examined. for hazard characterization ... | 1996 | 8856380 |
| assessment of alimentary exposure to listeria monocytogenes. | survey data on the frequency of foodborne occurrence and dietary exposure to listeria monocytogenes were used to estimate the mininmal mean per person annual rate of exposure in the united states during the late 1980s. the estimate was restricted to ready-to-eat (rte) foods because proper cooking was assumed to be listericidal. the mean amount of each food type per l. monocytogenes occurrence was calculated in about 100 sources, and dietary intake data were used to calculate the mean number of o ... | 1996 | 8856375 |
| hazard identification in swine slaughter with respect to foodborne bacteria. | swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ... | 1996 | 8856371 |
| functions of interleukin 1 receptor antagonist in gene knockout and overproducing mice. | interleukin 1 receptor antagonist (il-1ra) is a cytokine whose only known action is competitive inhibition of the binding of interleukin 1 (il-1) to its receptor. to investigate the physiological roles of endogenously produced il-1ra, we generated mice that either lack il-1ra or overproduce it under control of the endogenous promoter. mice lacking il-1ra have decreased body mass compared with wild-type controls. they are more susceptible than controls to lethal endotoxemia but are less susceptib ... | 1996 | 8855299 |
| comparison of the hydrophobic grid-membrane filter dna probe method and the health protection branch standard method for the detection of listeria monocytogenes in foods. | the standard health protection branch (hpb) method for the detection of l. monocytogenes in foods involves lengthy enrichment, selection and biochemical testing, requiring up to 8 days to complete. a hydrophobic grid-membrane filter (hgmf) method employing a digoxigenin-labelled listeriolysin o probe required 5 days to complete, and included an image-analysis system for electronic data acquisition. a total of 200 food samples encompassing 8 high-risk food groups (soft and semi-soft cheeses, pack ... | 1996 | 8854189 |
| mathematically modeling the repair of heat-injured listeria monocytogenes as affected by temperature, ph, and salt concentration. | heat-injured cells of listeria monocytogenes were inoculated into listeria repair broth (lrb) adjusted to various ph levels (4.2, 5.0, 6.6, 8.0 and 9.6) and salt concentrations (0.5%, 2.5%, 5.0%, 7.5% and 10.0% w/v) at controlled temperatures (4, 10, 22, 37 and 43 degrees c) in a complete factorial manner (5(3)). repair of the injured microorganisms was evaluated using selective and non-selective plating media. the gompertz parameters, which were generated by fitting the equation with the bacter ... | 1996 | 8854177 |
| antibacterial activity of lactobacillus plantarum ug1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin ug1. | lactobacillus plantarum ug1 isolated from dry sausage produced an antimicrobial substance that inhibited other strains of the genera lactobacillus and lactococcus, and some foodborne pathogens including listeria monocytogenes, bacillus cereus, clostridium perfringens and clostridium sporogenes. this antibacterial substance was inactivated by proteolytic enzymes and showed a bactericidal mode of action. consequently, it was characterized as a bacteriocin, and was designated plantaricin ug1. this ... | 1996 | 8854175 |
| quantitative structure activity relationship for the effect of benzoic acids, cinnamic acids and benzaldehydes on listeria monocytogenes. | the inhibition of a cocktail of 18 strains of listeria monocytogenes by 24 mono-, di- and tri-substituted benzoic and cinnamic acids and 16 benzaldehydes was evaluated using the concentration (c) required to give a 50% growth inhibition under anaerobic conditions at 35 degree c and ph 6.2 as a measure of biological activity (bav). using the method of least squares, multiple regression equations were obtained which described the contribution of some physiochemical and other structural properties ... | 1996 | 8852677 |
| cholesterol oxidase from rhodococcus equi is likely the major factor involved in the cooperative lytic process (camp reaction) with listeria monocytogenes. | the camp reaction between listeria monocytogenes and rhodococcus equi was studied by a diffusion assay. listeria monocytogenes displayed identical cooperative haemolytic effect with supernatant cultures of r. equi or with commercial cholesterol oxidase (cox). this result, even with enzymes of different sources (commercial cox is obtained from pseudomonas spp.) suggests that this enzyme secreted by r. equi has a crucial role in the synergistic haemolytic (camp) reaction with l. monocytogenes. the ... | 1996 | 8852354 |
| [an anti-listeria seroepidemiological study in uberaba, mg]. | from a total of 445 individuals, 17.1% had antibodies against l. monocytogenes detected by the agglutination tube test. they were separated in seven groups: bloods donnors (n = 50), hospital visitors (n = 40), frigorific workers (n = 28), aviculture workers (n = 87), herdsman (n = 31), agriculture students (n = 60) and street-sweepers (n = 51). l1/2a serotype was predominant. individuals from urban areas (19.5%) and those who had less contact with animals (21.7%) had significantly positive serol ... | 1996 | 8851214 |
| [survival of listeria monocytogenes in milk fermented with a starter culture for making yogurt]. | the purpose of this research was to determine the survival of listeria monocytogenes in sterile skim milk during the fermentation with a yogurt starter culture and during storage at refrigeration temperature. sterile skim milk was inoculated with 10(3), 10(5) and 10(7) cfu/ml of l. monocytogenes and with 10(6) cfu of lactic acid bacteria. inoculated milks were fermented for 8 h at 42 degrees c, followed by refrigeration at 4 degrees c. samples were taken at 2 h intervals during fermentation and ... | 1995 | 8850344 |
| [water disinfection: comparative activities of ozone and chlorine on a wide spectrum of bacteria]. | ozone and chlorine are agents that disinfect by destroying, neutralizing or inhibiting the growth of pathogenic microorganisms. the treatment of drinking water with ozone has shown to be more efficient against spores of bacillus subtilis. it was observed that the ozone already in dose of 0.35 mg/l produced the reduction of at least 5 log in populations of approximately 1 x 10(6) cells/ml of escherichia coli, vibrio cholerae, salmonella typhi, yersinia enterocolitica, pseudomonas aeruginosa, aero ... | 1995 | 8850129 |
| opsonin-independent adherence and phagocytosis of listeria monocytogenes by murine peritoneal macrophages. | listeria monocytogenes adhered to and multiplied intracellularly in murine peritoneal macrophages in the absence of opsonins. the infective process in these cells was evaluated by viable bacterial cell colony counts of intracellular organisms and documented by transmission and scanning electron microscopy. adherence of listeriae to macrophages involved surface interactions of the prokaryotic cell surface and eukaryotic cell membranes. subsequent phagocytosis was seen to occur through a process i ... | 1996 | 8849699 |
| listeria monocytogenes in poultry and poultry products: epidemiological investigations in seven danish abattoirs. | listeria monocytogenes was isolated from 11/236 (4 x 7%) caecal samples from parent flocks, providing broilers to the abattoirs investigated. caecal samples from 2078 broilers representing 90 randomly selected broiler flocks were negative for l. monocytogenes. a total of 3080 samples from seven abattoirs including poultry processing line samples, and final products were also examined for l. monocytogenes. listeria monocytogenes was isolated in 0 x 3% to 18 x 7% of the samples collected in the di ... | 1996 | 8849641 |
| comparison of antimicrobial susceptibilities of corynebacterium species by broth microdilution and disk diffusion methods. | corynebacterium species are increasingly being implicated in foreign-body infections and in immunocompromised-host infections. however, there are no specific recommendations on the method or the criteria to use in order to determine the in vitro activities of the antibiotics commonly used to treat corynebacterium infections. the first aim of our study was to compare the susceptibilities of various species of corynebacterium to vancomycin, erythromycin, and penicillin by using a broth microdiluti ... | 1996 | 8849254 |
| in vitro activities of u-100592 and u-100766, novel oxazolidinone antibacterial agents. | oxazolidinones make up a relatively new class of antimicrobial agents which possess a unique mechanism of bacterial protein synthesis inhibition. u-100592 (s)-n-[[3-[3-fluoro-4-[4-(hydroxyacetyl)-1-piperazinyl]- phenyl]-2-oxo-5-oxazolidinyl]methyl]-acetamide and u-100766 (s)-n-[[3-[3-fluoro-4-(4-morpholinyl)phenyl]- 2-oxo-5-oxazolidinyl]methyl]-acetamide are novel oxazolidinone analogs from a directed chemical modification program. mics were determined for a variety of bacterial clinical isolate ... | 1996 | 8849237 |
| bioluminescence measurements of the antilisterial activity of nisin: comparison with ampicillin and streptomycin. | the influence of nisin on intracellular atp and cell numbers of listeria monocytogenes strain scott a was determined and compared with the effect of amplicillin and streptomycin under similar conditions. in the presence of nisin (3-12 micrograms/ml), intracellular atp and cell numbers decreased rapidly during the first hour at 35 degrees c and extracellular atp increased. cell numbers and intracellular atp increased after 3 h of incubation. no effect was observed in cells treated with ampicillin ... | 1996 | 8844346 |
| listeriosis in bone marrow transplant recipient. | 1996 | 8842304 | |
| differential induction of il12 synthesis by mycobacterium bovis bcg and listeria monocytogenes. | 1995 | 8839156 | |
| listeria monocytogenes infection in a patient treated with methotrexate for rheumatoid arthritis. | 1996 | 8838541 | |
| effect of immunomodulation with galactoside-specific mistletoe lectin on experimental listeriosis. | balb/c-mice (n = 10 per experimental group) were intravenously infected with 5 x 10(4) and 5 x 10(5) viable cells of listeria monocytogenes slcc 4013. about 50-80 h after this challenge, all mice of the untreated control groups succumbed to their infection. pretreatment of experimental animals on the optimal immunoactive schedule with the galactoside-specific mistletoe lectin (ml-1; 1 ng/kg body weight; days 1, 4, 5, 6 before challenge), however, evidently reduced the lethality of listerial infe ... | 1996 | 8837402 |
| protein p60 participates in intestinal host invasion by listeria monocytogenes. | the role of p60 in intestinal invasion by listeria monocytogenes was assessed after oral infection of mice with the p60 low-expressing mutant riii, or with anti-p60 antibody coated wild-type egd. invasion by l. monocytogenes riii bacteria has been unimpaired suggesting that a low density of p60 suffices for entry. up to 24 h post infection (p.i.), intestinal penetration by l. monocytogenes egd bacteria was markedly reduced by coating with anti-p60 antibodies. in histological sections, anti-p60 a ... | 1996 | 8837387 |
| interferon-gamma and interleukin-10 have cross-regulatory roles in modulating the class i and class ii mhc-mediated presentation of epitopes of listeria monocytogenes by infected macrophages. | ifn-gamma is an important cytokine in resistance to infection with listeria monocytogenes, and interleukin 10 is known to exacerbate infection with listeria and other intracellular pathogens. we examined the effects of these cytokines on antigen presentation by macrophages infected with live listeria. listeriolysin o, a hemolysin secreted by listeria, is an immunodominant antigen presented by both class i and class ii mhc on infected cells. thioglycollate-elicited macrophages were pretreated wit ... | 1996 | 8836921 |
| the focal-adhesion vasodilator-stimulated phosphoprotein (vasp) binds to the proline-rich domain in vinculin. | in mammalian cells vasodilator-stimulated phosphoprotein (vasp) is localized to focal adhesions and areas of dynamic membrane activity where it is thought to have a role in actinfilament assembly. the proteins responsible for recruiting vasp to these sites within the cell are not known. the bacterial protein acta binds vasp via a proline-rich motif that is very similar to a sequence in the proline-rich region of the focal-adhesion protein vinculin. we have examined the ability of vasp, synthesiz ... | 1996 | 8836115 |
| activity of ci-960 alone and in combination with amoxycillin against listeria monocytogenes, and comparison with other quinolones. | in-vitro activity of ci-960 against seven strains of listeria monocytogenes was compared to that of other fluoroquinolones. mics/mbcs were: ciprofloxacin and sparfloxacin: 0.5-2/1-4 mg/l;pd 131628: 0.125-0.5/0.25-2 mg/l; ci-960: 0.06-0.25/0.25-0.5 mg/l. at 4 h, ci-960 (4 x mic) reduced the inoculum (log10 cfu) by 2.88 +/- 0.61 compared with 1.78 +/- 0.48 for ciprofloxacin, 1.5 +/- 0.46 for sparfloxacin, and 1.35 +/- 0.47 for pd 131628 (p < 0.05). at 24 h, the bactericidal effects were similar (- ... | 1995 | 8830017 |
| the use of listeriolysin o in an elisa, a skin test and a lymphocyte blastogenesis assay on sheep experimentally infected with listeria monocytogenes, listeria ivanovii or listeria innocua. | purified listeriolysin o (llo) was evaluated as a specific antigen to detect both humoral and cell mediated immune responses of sheep infected with listeria monocytogenes. six sheep (two in each group) were orally inoculated with 10(10) organisms of l. monocytogenes, l. ivanovii, or l. innocua. only the l. monocytogenes inoculated sheep had an elevated temperature (> 42 degrees c) and after 15 days had anti-llo antibodies as assessed by an elisa. in a blastogenesis assay, only peripheral blood m ... | 1996 | 8828131 |
| intracellular survival of enteric bacteria in cultured human enterocytes. | translocating enteric bacteria have been visualized within intact intestinal epithelial cells in animal models of bacterial translocation. although the ability of the enterocyte to engulf bacteria has been well documented in both in vivo and in vitro experimental models, relatively little is known about the enterocyte's ability to degrade internalized bacteria. intracellular survival of eight strains of enteric bacteria (two strains of listeria monocytogenes, salmonella typhimurium, proteus mira ... | 1996 | 8828081 |
| mri appearances of listeria rhombencephalitis. | listeria monocytogenes is a rare cause of central nervous system infection in the non-immunocomprised patient and 10% of these patients develop a rhombencephalitis. we present such a case and discuss the clinical, pathological and radiological features. | 1996 | 8826752 |
| modulation of dna topology by flar, a new gene from listeria monocytogenes. | we report the identification of a previously unknown listeria monocytogenes gene, flar, which modulates dna topology. through the analysis of a tn917 non-motile mutant, losc1, in which production of flagellin was abolished, we have identified a bacterial component involved in gene regulation. the transposon had inserted in flar, an open reading frame of 531 bp, followed by a second open reading frame of 1252 bp in reverse orientation. on the l. monocytogenes physical map, flar was located in a d ... | 1995 | 8825084 |
| serotyping and esterase typing for analysis of listeria monocytogenes populations recovered from foodstuffs and from human patients with listeriosis in belgium. | listeria monocytogenes strains isolated in belgium from different foodstuffs and in sporadic cases of human listeriosis were analyzed. the distribution of serovars differed in each of these populations. the bacteria isolated from cheeses and from human patients with listeriosis were further studied by esterase typing. the twenty esterase patterns defined were not equally distributed in these two populations. the secretion of the virulence determinant phosphatidylinositol-specific phospholipase c ... | 1996 | 8815071 |
| copper and zinc exposure of zebrafish, brachydanio rerio (hamilton-buchaman): effects in experimental listeria infection. | to investigate the effects of heavy metals on susceptibility of fish to listeriosis, normal zebrafish, brachydanio rerio (hamilton-buchanan), were exposed to varying concentrations of zinc (0.05, 0.15, and 0.25 mg/liter) and copper (0.05, 0.10, and 0.15 mg/liter). during copper exposure, this heavy metal did not accumulate in zebrafish kidney. unlike copper, a small amount of zinc accumulated in kidneys of fish exposed at 0.25 mg/liter. to estimate the effects of this heavy metal on listerial in ... | 1996 | 8812178 |
| differential regulation of the virulence genes of listeria monocytogenes by the transcriptional activator prfa. | the two listeria monocytogenes strains egd and nctc 7973 display a different regulation pattern of their prfa-dependent genes. all prfa-dependent genes from l. monocytogenes nctc 7973 are much more efficiently transcribed in brain-heart infusion medium than those from strain egd. transcription of these genes in egd is, however, induced after shift into minimal essential medium (mem) to a level that is comparable to that of strain nctc 7973. expression of the internalin gene (inla) is also influe ... | 1996 | 8809771 |
| enhanced in vitro engulfment of listeria monocytogenes by rabbit polymorphonuclear leukocytes in the presence of sera from immune rabbits. | the role of immune serum in the engulfment of listeria monocytogenes by polymorphonuclear leukocytes (pmnls) of rabbits was documented employing an in vitro assay. three serovarieties of l. monocytogenes, viz. serovars 1/2a, 4a and 4b, were separately incubated with pmnls of nonimmune rabbit and high titre homologous or heterologous antisera. normal rabbit serum was used as control. the number of l. monocytogenes per neutrophil was counted in stained smears in each assay and opsonic indices were ... | 1996 | 8809545 |
| il-10 production by adult human derived microglial cells. | microglia, a population of central nervous system (cns) macrophages, have been demonstrated to support immune accessory and effector functions in the cns. numerous studies support the role of microglia in cns development and pathology, where activation of microglia is consistently noted. the current study investigated microglial immune functions under basal and activation conditions and assessed the ability of interleukin-10 (il-10), added exogenously or produced by microglia, to down-regulate m ... | 1996 | 8808789 |
| simultaneous detection of listeria spp. and listeria monocytogenes by reverse hybridization with 16s-23s rrna spacer probes. | enzymatic amplification results showed that listeria species have at least two 16s-23s rrna spacer regions of different lengths. these spacer regions of l. monocytogenes, l. ivanovii and l. seeligeri were cloned after enzymatic amplification. sequence analysis of the inserts revealed two spacers of 245-246 bp and 496-498 bp, respectively, of which the latter included trna(ala) and trna(ile) genes. one listeria spp.-specific probe, lis-icg4, was deduced from the 245-bp spacer and a l. monocytogen ... | 1995 | 8808313 |
| suppression of ifn-gamma production from listeria monocytogenes-specific t cells by endogenously produced nitric oxide. | the induction of nitric oxide (no) by ifn-gamma has been well documented in a variety of experimental settings, but so far there has been no report on whether the endogenously produced no can suppress ifn-gamma production. in the present study, cd4+ t cells from listeria monocytogenes-immune mice produced ifn-gamma upon stimulation with specific antigen and no was generated in culture. when ng-monomethyl-l-arginine (nmma) was added to the culture at a dose sufficient for the complete blockade of ... | 1996 | 8806814 |
| bacteria in the bloodstream are trapped in the liver and killed by immigrating neutrophils. | the critical role of the liver in the resolution of systemic bacterial infections is well documented. in the case of listeria monocytogenes, approximately 60% of bacteria inoculated i.v. into mice are recovered in the liver at 10 min after infection. here we report that the listeria recovered at 10 min were distributed equally among the hepatocyte and nonparenchymal liver cell populations. the majority (>/= 75%) of these organisms were bound extracellularly as judged by their sensitivity to gent ... | 1996 | 8805652 |
| common entry mechanisms. bacterial pathogenesis. | the recent identification of e-cadherin as the cell-surface receptor for the listeria monocytogenes adhesin, internalin, indicates that pathogenic bacteria exploit a variety of host surface molecules for entry and that they may share some common strategies for uptake into host cells. | 1996 | 8805360 |
| live recombinant listeria monocytogenes as an immunotherapeutic agent for experimental tumors. | 1995 | 8800833 | |
| listeria monocytogenes meningitis complicated by symptomatic spinal subarachnoid cysts. | we report the case of a man who developed signs of compression of the spinal cord in the dorsal region over a year after treatment of listeria monocytogenes meningitis. he proved to have mild hydrocephalus and a number of subarachnoid pouches compressing the upper dorsal cord. insertion of a ventriculo-peritoneal shunt caused clinical improvement. we believe that this was caused by relief of tension in the spinal cysts rather than any reduction in ventricular size. | 1996 | 8799548 |
| use of aqueous two-phase systems in sample preparation for polymerase chain reaction-based detection of microorganisms. | an aqueous two-phase system, consisting of poly(ethylene glycol) (peg) and dextran, was employed to separate polymerase chain reaction (pcr)-inhibitory substances from bacterial cells. the pcr inhibition of four soft cheeses was examined and three of them were found to be strongly pcr-inhibitory. extraction of the pcr-inhibitory soft cheeses inoculated with listeria monocytogenes in an aqueous two-phase system containing 8% (w/w) peg 4000 and 8% (w/w) dextran 500, was found to lower the pcr dete ... | 1996 | 8798894 |
| cerebritis due to listeria monocytogenes: ct and mr findings. | infections by listeria monocytogenes are uncommon, with cerebritis being even rarer. we present three cases of cerebritis which occurred during an outbreak of listeriosis. the ct and mr findings at diagnosis and during follow-up are described. predominant deep white matter lesions with nodular and ring enhancement were seen. the mr yielded a higher resolution of the lesions than ct. | 1996 | 8797977 |
| effect of monolaurin and lactic acid on listeria monocytogenes attached to catfish fillets. | the purpose of this study was to determine the effects of monolaurin and lactic acid, singly or combined, on listeria monocytogenes attached to catfish fillets. skinless catfish fillets were inoculated with l. monocytogenes and dip treated in monolaurin and/or lactic acid solution for various time periods. results showed that monolaurin up to 400 micrograms/ml had no influence on counts. conversely, lactic acid-treated fillets had reduced counts compared to controls. dipping in 0.85, 1.70, or 2. ... | 1996 | 8796441 |
| control of listeria monocytogenes in ground beef by 'lactocin 705', a bacteriocin produced by lactobacillus casei crl 705). | the effectiveness of lactobacillus casei crl 705 as well as that of lactocin 705, the associated bacteriocin produced, in reducing population levels and growth of listeria monocytogenes in sterile and non-sterile ground beef was studied. predetermined numbers of l. monocytogenes and concentrations of lactocin 705 were added to the meat slurries. listeria in the bacteriocin-treated and control samples during incubation at 20 degrees c were enumerated as cfu on bacto blood agar base. results indic ... | 1996 | 8796440 |
| production and characterization of enterocin 900, a bacteriocin produced by enterococcus faecium bfe 900 from black olives. | enterococcus faecium bfe 900 isolated from black olives produced a bacteriocin termed enterocin 900, which was antagonistic towards lactobacillus sake, clostridium butyricum, enterococci as well as listeria spp. including listeria monocytogenes. enterocin 900 was inactivated by pepsin, alpha-chymotrypsin, proteinase k and trypsin but not by catalase, alpha-amylase, or other non-proteolytic enzymes tested. the bacteriocin was heat stable, retaining activity after heating at 121 degrees c for 15 m ... | 1996 | 8796427 |
| the particular behaviour of listeria monocytogenes under sub-optimal conditions. | listeria monocytogenes is a ubiquitous pathogenic microorganism which has been described as growing at temperatures of interest to food production and especially at low temperatures (-2 degrees to 8 degrees c) in storage process. however, the general relationship between the maximum specific growth rate, mumax and temperature has not often been studied for l. monocytogenes in the whole temperature range from minimal to maximal growth temperature. a global analysis of this relationship for temper ... | 1996 | 8796423 |
| acid adaptation of listeria monocytogenes can enhance survival in acidic foods and during milk fermentation. | we have previously shown that tolerance to severe acid stress (ph 3.5) can be induced in listeria monocytogenes following a 1-h adaptation to mild acid (ph 5.5), a phenomenon termed the acid tolerance response (atr) (b. o'driscoll, c. g. m. gahan, and c. hill, appl. environ. microbiol. 62:1693-1698, 1966). in an attempt to determine the industrial significance of the atr, we have examined the survival of adapted and nonadapted cells in a variety of acidic foods. acid adaptation enhanced the surv ... | 1996 | 8795199 |
| role of osmolytes in adaptation of osmotically stressed and chill-stressed listeria monocytogenes grown in liquid media and on processed meat surfaces. | listeria monocytogenes is a food-borne pathogen that is widely distributed in nature and is found in many kinds of fresh and processed foods. the pervasiveness of this organism is due, in part, to its ability to tolerate environments with elevated osmolarity and reduced temperatures. previously, we showed that l. monocytogenes adapts to osmotic and chill stress by transporting the osmolyte glycine betaine from the environment and accumulating it intracellularly (r. ko, l. t. smith, and g. m. smi ... | 1996 | 8795194 |
| primary and secondary immune responses to listeria monocytogenes. | recent studies have revealed the complexity of cytokine and cellular interactions required for resistance to primary listeria monocytogenes infection and have illustrated that resistance to secondary infection may occur through multiple pathways. analyses of listeria epitope generation and the specificity of protective cd8(+) t cells have suggested that future research should focus on secreted protein antigens in specific resistance to infection and have increased our understanding of listeria a ... | 1996 | 8794012 |
| a single substitution in the putative helix-turn-helix motif of the pleiotropic activator prfa attenuates listeria monocytogenes virulence. | prfa, the regulator of virulence-gene expression in the pathogenic bacterium listeria monocytogenes, displays sequence similarity to members of the cap-fnr family of transcriptional regulators. to test the functional significance of this similarity, we constructed and analysed substitutions of two amino acids of prfa predicted to contact dna, i.e. ser-184 and ser-183. substitution of ser-184 by ala reduced dna binding and virulence-gene activation, and attenuated the virulence in a mouse model o ... | 1996 | 8793875 |
| activation of natural killer cells by heat-killed listeria monocytogenes requires additional signals from lymphoid cells. | regulatory and protective functions have been attributed to murine natural killer (nk) cells in a number of infectious diseases including listeriosis. we have developed an in vitro model to study parameters underlying the activation of naive nk cells using heat-killed listeria monocytogenes (hkl) as stimulator. independent from expression of the cell surface marker nk1.1, nk cells lysed yac-1 cells after in vitro stimulation with hkl or hkl + interleukin (il)-2, but not medium or il-2 alone. in ... | 1996 | 8793563 |
| functional t cell subsets in mycobacterial and listerial infections: lessons from other intracellular pathogens. | 1996 | 8791719 | |
| investigation of the effect of combined variations in temperature, ph, and nacl concentration on nisin inhibition of listeria monocytogenes and staphylococcus aureus. | gradient plates were used to investigate the effects of varying temperature, ph, and sodium chloride (nacl) concentration on nisin inhibition of staphylococcus aureus and listeria monocytogenes, nisin was incorporated into the plates of 0, 50, 100, 250, and 500 iu ml -1. gradients of ph (3.7 to 7.92) at right angles to nacl concentration (2.1 to 7% [wt/vol]) were used for the plates, which were incubated at 20, 25, 30 and 35 degrees c. growth on the plates were recorded by eye and by image analy ... | 1996 | 8787399 |
| [antimicrobial activities of meropenem against clinically isolated strains. the result against strains isolated from blood and cerebrospinal fluid]. | in order to evaluate the antimicrobial activity of meropenem (mepm), minimum inhibitory concentrations (mics) of mepm and control drugs were determined against clinical isolates from blood and cerebrospinal fluid that were obtained from january, 1993 to december, 1994. the results are summarized as follows; 1. the mic-range, 50% mic (mic50) and 90% mic (mic90) of mepm were equal to those of imipenem (ipm) and panipenem (papm) against streptococcus pneumoniae including benzylpenicillin (pcg)-inse ... | 1996 | 8786628 |
| diversity of the polar lipids of the food-borne pathogen listeria monocytogenes. | listeria monocytogenes is a gram-positive bacterium that can adapt to high salinity and cold. because the membrane lipids may play a role in its survival and adaptation, we have examined the polar lipids of l. monocytogenes. extraction of total lipids from l. monocytogenes yielded 7 +/- 1 mg/ml wet cells. polar lipids represented 64% of total lipids and contained 9% lipid-phosphorus. polar lipids were separated into 14 components by two-dimensional thin-layer chromatography. eight components (88 ... | 1996 | 8784744 |
| taxonomic note: a proposal for reviewing the interpretation of the camp reaction between listeria monocytogenes and rhodococcus equi. | the discrepancies between the current description of the camp test between listeria monocytogenes and rhodococcus equi in the latest edition of bergey's manual of determinative bacteriology (l. monocytogenes is described as camp test negative with r. equi) and routine findings (positive reactions are usually described in many laboratories) make it advisable to review the current interpretation of the camp test to avoid confusion among people working in microbiological laboratories. overall, 98.4 ... | 1996 | 8782698 |
| comparative analysis of 16s and 23s rrna sequences of listeria species. | in order to establish the taxonomic value of 16s rrna and 23s rrna for distinguishing listeria species, the complete 23s rrna sequences for all listeria species were determined by using the type strains. we designed and experimentally validated a universal 23s rrna sequencing method, which included pcr amplification of the rdna gene and direct cycle sequencing of the amplicon with eubacterial primers. the results of our sequence comparison indicated that the genus listeria can be divided into tw ... | 1996 | 8782674 |