Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| campylobacter strains from swedish patients with diarrhoea. distribution of serotypes over a five year period. | serotyping, hs antigen and hl antigen, was performed on 105 campylobacter jejuni/coli isolates from the same number of consequtive patients seeking medical attention for diarrhoea. the results were compared to a similar study performed five years earlier. it was found that there were only minor differences in frequency of the serotypes commonly isolated during the two different periods. | 1989 | 2713133 |
| [antibiotic sensitivity of campylobacter jejuni and campylobacter coli strains with special reference to sensitivity to erythromycin and neomycin]. | 166 strains of c. jejuni and 46 strains of c. coli were typed against 14 antibiotics using the agar diffusion test. all strains were resistant against penicillin g, oxacillin and rifampicin. susceptibility were found against chloramphenicol, erythromycin, neomycin, oxytetracyclin, streptomycin and gentamycin. the minimal inhibition concentration was performed for erythromycin and neomycin. all strains tested were inhibited by less than or equal to 2 micrograms/ml neomycin, one strain was resista ... | 1989 | 2710556 |
| effect of campylobacter jejuni extracts and culture supernatants on cell culture. | this study reports the effect of culture supernatants and extracts of campylobacter jejuni isolated in bangladesh from patients with gastroenteritis, asymptomatic carriers, poultry, and animals on tissue culture system. isolates from patients produced morphologic changes on hela, vero and y1 adrenal cell lines. incorporation of polymyxin b in the culture medium enhanced the effect on cell lines significantly and sonicated extracts of the organisms had an even more significant effect. almost all ... | 1989 | 2709480 |
| an appraisal of the efficacy of pre-enrichment for the isolation of campylobacter jejuni from water and food. | cells of campylobacter jejuni exposed to heating or freezing were progressively less able to grow at 43 degrees c, particularly on selective media. this influenced the recovery of damaged cells from naturally and artificially contaminated samples. with broth culture the isolation rate could be increased by pre-enrichment in basal or selective media at 37 degrees c for 4 h. with membrane filtration or surface plating techniques the inclusion of agents that quench toxic derivatives of oxygen was m ... | 1989 | 2708169 |
| experimental campylobacter jejuni infection in macaca nemestrina. | experimental infection of four specific-pathogen-free macaca nemestrina monkeys (aged 3.5 and 4.5 months) with campylobacter jejuni 81-176 caused acute diarrheal illness, characterized by fluid diarrhea, bloody stools, and fecal leukocytes, which lasted for approximately 7 to 11 days. histologic examination of intestinal biopsies showed acute colitis characterized by infiltration of the mucosa with neutrophils and lymphocytes, and cryptitis. there were no histologic changes in the small intestin ... | 1989 | 2707853 |
| population genetics of human and animal enteric campylobacter strains. | a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ... | 1989 | 2707852 |
| comparison of different vaccines and induced immune response against campylobacter jejuni colonization in the infant mouse. | the degree of protection conferred by vaccinated dams on infant mice against colonization by campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. in some instances of homologous protection, serum bactericidal titres correlated well with protection. however, boiled c. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. conversely, bactericidal activity could not be demonstrated against strains ... | 1989 | 2703020 |
| serotyping studies of campylobacter from naturally colonized chickens. | campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ... | 1989 | 2703018 |
| [bacterial etiological factors of diarrhea in the stool of infants hospitalized in january 1987]. | the results are presented of the second part of the studies conducted for monitoring of the variability of bacterial factors causing diarrhoea in the youngest children. the presently reported study comprised 42 babies aged from 7 days to 18 months treated in hospital in january 1987. the number of children with diarrhoea hospitalized in this period was relatively smaller than in january 1985, while the incidence of enteropathogenic bacteria in stools was very similar. they included mainly klebsi ... | 1989 | 2701337 |
| [a survey on campylobacter jejuni high infection in a nursery]. | an epidemiologic survey on campylobacter jejuni infection in a nursery was made in april 1987. it was found that there was a spread of c. jejuni high infection. two cooks affected by enteritis of c. jejuni were considered to be a reservoir of the infection. stool cultures were positive for c. jejuni in 43 out of 187 children, with a positive rate of 23%. eight of the 43 had diarrhea and the others were asymptomatic carriers (24.3%). controls were set up in another nursery with 238 healthy childr ... | 1989 | 2698371 |
| campylobacter fetus subsp. jejuni in poultry reared under different management systems in nigeria. | cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for campylobacter fetus subsp. jejuni. it was isolated from 12.3% of the birds in 19 flocks. chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. it was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect r ... | 1989 | 2695052 |
| campylobacter infection in humans. | campylobacter jejuni and campylobacter coli are now recognized as common bacterial cause of enteritis in humans. they infect the healthiest of persons resulting in a self-limiting diarrhoea often accompanied by mucus and blood in the stool. on the other hand c. fetus subsp fetus attacks otherwise debilitated persons whose body defences have been weakened by underlying conditions, and causes extragastrointestinal campylobacteriosis. c. pylori, which is able to survive in the stomach acid environm ... | 1989 | 2693070 |
| use of eryc capsule in adult patients. | ninety adult patients were treated with eryc capsule, 1-2 capsules (250-500 mg) were given every 6 hours one hour before meals. the treatment was continued for 4-14 days, in cases of upper and lower respiratory tract infections, cutaneous and soft part infections, borreliosis and enteritis caused by campylobacter jejuni. of the 90 patients 85 recovered, 2 improved, in 3 cases failure was recorded. side-effect necessitating the discontinuance of therapy was observed only in one case. | 1989 | 2686082 |
| effect of endotoxin on colonisation of campylobacter jejuni in infant mice. | an infant mouse model has been used to investigate the colonisation of the intestine by campylobacter jejuni and the effect of endotoxin (escherichia coli o26: b6) on the initial stage of this process. endotoxin injected 1 or 16 h before the bacterial challenge had no effect on the growth of campylobacters but endotoxin injected 4 to 10 h before the bacterial challenge caused a bacteriostatic effect on the growth of campylobacters which lasted for one day. the bacteriostatic effect was evident b ... | 1989 | 2685315 |
| applications of a new method for devising bacteriological serotyping schemes. | greatly simplified serotyping schemes were devised for two organisms, escherichia coli and campylobacter jejuni. the mathematical approach developed to improve the e. coli serotyping routine at the national health institute in new zealand was used on three previously published data sets. it had been considered possible that the mathematical method used in the first study was specific to that data set and therefore not applicable elsewhere. this paper verifies that the mathematical method works o ... | 1989 | 2685036 |
| a study of campylobacter jejuni and campylobacter coli in a river system. | campylobacter isolates were detected in 82.1% of river water samples moderately polluted with organic wastes. in the majority of the samples less than 10 campylobacter cfu/100 ml were detectable. the presence of waterfowl and the faecal contamination from a poultry-farm resulted in considerable higher campylobacter contents (greater than 240/100 ml). correlation studies showed a high correlation coefficient (p less than 0.05) between the number of campylobacters and that of total coliforms. camp ... | 1989 | 2684204 |
| serotypes & biotypes of campylobacter jejuni & c. coli from diverse sources in calcutta. | c. jejuni and c. coli isolates from diverse sources in calcutta were serotyped and biotyped according to the lior scheme. of the 55 strains examined, 85.5 per cent reacted with one or another of the 73 antisera available. this included the formation of two new serogroups, lio 67 and lio 76. c. coli serogroup lio 46 biotype ii was the most frequently encountered strains (14.5%), followed by c. coli serogroup lio 29, 55 biotype ii (10.5%) and c. jejuni serogroup lio 54, biotype i (5.5%). serogroup ... | 1989 | 2680933 |
| [antibacterial and bactericidal activities of japanese green tea]. | we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ... | 1989 | 2677434 |
| a one-year study on thermophilic campylobacters isolated from fecal specimens. | during a 12-month period, a systematic screening for thermophilic campylobacters was done on 5723 stool samples of hospitalized patients and out-patients who required stool culture tests for gastrointestinal disturbances. campylobacter strains were isolated in 130 samples (2.27%) and campylobacter jejuni (mainly biotype ii) was the species most frequently isolated. no pronounced seasonal pattern was evident and the age at which infection was more often observed was that from 6 to 24 months. eryt ... | 1989 | 2674635 |
| etiology of childhood diarrhea in korea. | to assess the role of recently recognized enteropathogens in childhood diarrhea in korea, 231 children with diarrhea admitted to and 104 children without diarrhea seen at the well-baby clinic or the outpatient department of hanyang university hospital in seoul, korea, were evaluated during a 14-month period. stools were cultured for bacterial pathogens, including enterotoxigenic (heat-labile and heat-stable enterotoxin-producing) and enteroadherent organisms. only those stools obtained from pati ... | 1989 | 2666437 |
| [neurologic involvement in campylobacter infections. 5 cases]. | campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ... | 1989 | 2664975 |
| [susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates]. | susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ... | 1989 | 2664255 |
| microbiologic and clinical study of acute diarrhea in children in aswan, egypt. | children with diarrhea presenting to a government rehydration center in aswan, egypt, were investigated to determine the etiology and clinical presentation of acute childhood diarrhea in southern egypt. among 126 outpatients and 25 inpatients with diarrhea (mean age 18 months), enterotoxigenic escherichia coli (etec) (17% of cases), cryptosporidium (9%), salmonella spp. (7%), campylobacter jejuni/coli (7%), and shigella spp. (5%) were the most common enteropathogens identified during the high in ... | 1989 | 2658018 |
| enteroaggregative escherichia coli associated with persistent diarrhea in a cohort of rural children in india. | a cohort of 452 rural children was followed longitudinally for 13 mo to ascertain the role of hep-2 cell adherent escherichia coli and other pathogens in causing acute (less than or equal to 14 d) and persistent (greater than 14 d) diarrhea. aeromonas, campylobacter jejuni, e. coli manifesting localized adherence to hep-2 cells and enterotoxigenic e. coli were significantly associated with acute diarrhea. e. coli strains that exhibit aggregative adherence, so-called enteroaggregative e. coli, a ... | 1989 | 2656875 |
| campylobacter jejuni chromosomal sequences that hybridize to vibrio cholerae and escherichia coli lt enterotoxin genes. | campylobacter jejuni is one of the main etiologic agents of gastrointestinal illness in developing and developed areas throughout the world. isolation of enterotoxin-producing c. jejuni has been associated with clinical symptoms of a watery-secretory type of diarrhea. although physiological and immunological relatedness has been demonstrated between the c. jejuni enterotoxin (cjt), the vibrio cholerae enterotoxin (ct), and the heat-labile cholera-like escherichia coli enterotoxin (lt), nucleotid ... | 1989 | 2653963 |
| [isolation of campylobacter jejuni and campylobacter coli from children with acute intestinal diseases]. | in this work the data obtained in the examination of 338 children with diarrhea, aged 5 days to 14 years, are presented. the methods used for the collection of samples and their storage till the moment of inoculation are described. the possibility of using the microscopic examination of campylobacter-containing native feces is shown. the work resulted in the isolation of 85 c. jejuni and c. coli strains. as shown in this work, the isolation of campylobacter depended on the age of children and th ... | 1989 | 2652954 |
| characterization and antibiotic sensitivity of campylobacter jejuni and campylobacter coli isolated from children at the lagos university teaching hospital (luth), lagos, nigeria. | one hundred and thirteen rectal swabs and 87 faecal samples obtained from the paediatric casualty of the lagos university teaching hospital, lagos, nigeria were processed for campylobacter jejuni/coli. twenty-two (11%) of the specimens were positive for c. jejuni. biotyping results showed that nine (41%) and eight (36%) belonged to c. jejuni biotypes i and ii respectively, while three (14%) and two (9%) belonged to c. coli biotypes i and ii. serotyping results revealed that c. jejuni biotypes i ... | 1989 | 2651685 |
| incidence and etiology of infantile diarrhea and major routes of transmission in huascar, peru. | community-based studies of diarrhea etiology and epidemiology were carried out from july 1982-june 1984 in 153 infants residing in a poor peri-urban community near lima, peru. study infants had nearly 10 episodes of diarrhea in their first year of life. diarrhea episodes were associated with organisms such as campylobacter jejuni, enterotoxigenic and enteropathogenic escherichia coli, shigella, rotavirus, and cryptosporidium. these organisms appeared to be transmitted to infants in the home thro ... | 1989 | 2646919 |
| emerging role of quinolones in the prevention of gram-negative bacteremia in neutropenic cancer patients and in the treatment of enteric infections. | the pharmacokinetic and antibacterial characteristics of the fluorinated carboxyquinolones make them attractive candidates for the treatment and prevention of infections arising from the alimentary canal. norfloxacin and ciprofloxacin have been shown to suppress and eliminate the pool of potentially pathogenic aerobic gram-negative rods colonizing the alimentary canal of neutropenic patients with acute leukemia, thereby reducing infection-related morbidity and mortality due to gram-negative seps ... | 1989 | 2646056 |
| better growth of campylobacter jejuni using simple fortner's principle & candle extinction jar. | c. jejuni isolated from patients of childhood diarrhoea and chicken intestines were examined for better growth in both liquid and solid media under three different environments of candle extinction jar with or without an escherichia coli inoculated plate and three such plates in a sealed jar. better growth by one log in liquid media was obtained at 24 and 48 h with bacterial counts of about ca. 10(7) and ca. 10(9) respectively, and larger colony size at 24 h on solid medium by inclusion of a sin ... | 1989 | 2644169 |
| [serological diagnosis of campylobacter jejuni infections]. | antibody level to campylobacter in 28 sera of patients of whom campylobacter infection was confirmed by germ isolation from feces was tested. the investigation was performed using passive haemagglutination technique and as antigens heated and acid glycine extraction prepared from homologous and reference strains. for the method used the heated antigen proved to be superior. out of 28 tested patients of whom 92.8% were children, 8 sera were positive, 9 doubtful and 9, derived mainly from neonates ... | 1989 | 2640683 |
| [frequency and characteristics of campylobacter jejuni-coli diarrhea in tunisia]. | because importance of campylobacter jejuni/coli in human disease, we studied frequency, clinical and biological symptoms of campylobacter enteritis. investigation has been realised on 216 cases of diarrhoea seen outside the hospital and aged from 4 days to 60 years, and 512 cases of hospitalised diarrhoea. outside the hospital, this bacteria has the same frequency as shigella and salmonella (3-5%). inside the hospital, it is rare. outside the hospital, this bacteria is isolated in babies and chi ... | 1989 | 2633873 |
| bacterial and viral causes of acute diarrhoea in children in kuwait. | bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ... | 1989 | 2632640 |
| endotoxic activity and enterotoxigenicity of human strains of campylobacter jejuni isolated from patients in a nigerian hospital. | limulus gelation assay and dermal schwartzman reaction provided a sensitive and reproducible means of testing the endotoxic activity of hospital strains of campylobacter jejuni in lagos, nigeria. all the 22 isolates of campylobacter jejuni tested for the limulus gelation assay were positive for the production of endotoxin. furthermore, the campylobacter suspensions caused a positive dermal schwartzman reaction in rabbits. the area of skin reaction was less extensive than that produced by escheri ... | 1989 | 2632005 |
| systemic spread of campylobacter jejuni after intravenous infections. | mice were infected intravenously with campylobacter jejuni in order to study systemic translocation of this vibrio, as well as the interactions between bacteria and the host's defense mechanisms. it was found that granulocytes phagocyte c. jejuni in the bloodstream and that phagocytosis could be stimulated with lps-pretreatment or, less effectively, opsonizing antibodies. it could also be demonstrated that these circulating 'infected' granulocytes are eliminated from the bloodstream mostly by th ... | 1989 | 2631866 |
| glomerulonephritis, pulmonary hemorrhage and anemia associated with campylobacter jejuni infection. | a 5-year-old girl suffered campylobacter jejuni enteritis. over the ensuing weeks she developed glomerulonephritis, pulmonary hemorrhage and anemia. renal biopsy revealed immune-complex mediated, crescentic glomerulonephritis. campylobacter jejuni antigen was identified in the glomeruli suggesting a causal role of campylobacter jejuni in the disease process. | 1989 | 2631667 |
| [cytotoxin adhesion and invasiveness of campylobacter jejuni from children with diarrhea: assay and correlation with clinical manifestations]. | some potentially pathogenic properties have been identified for campylobacter jejuni in vitro. the cell-free filtrates of 42 strains, (28 isolated from clinical cases of diarrhea, 12 from domestic animals and 2 from healthy children) were investigated. 30 of the 42 were cytotoxin producers, and the strains from clinical cases were more frequent than those from other sources (p less than 0.05). meanwhile, 25 of the 42 strains exhibited adhesion and invasiveness with hela cell line in vitro. it is ... | 1989 | 2630022 |
| prevalence of campylobacter jejuni in selected domestic and wild birds in louisiana. | prevalence of campylobacter jejuni was determined in a selected population of domestic and free-living birds submitted for necropsy to the louisiana state veterinary medical diagnostic laboratory. the 445 cases examined included 13 orders of birds and yielded c. jejuni in 45 cases, representing an isolation rate of 10.1%. prevalence was highest in galliformes (25.2%), followed by anseriformes (12.9%) and columbiformes (8.3%). only one isolation was made out of 179 psittaciformes examined. penner ... | 1989 | 2619661 |
| adhesion to hela cells of campylobacter jejuni and c. coli outer membrane components. | adhesion of campylobacter jejuni and c. coli to epithelial cells is thought to be a decisive step in enteritis. in this work, we tried to determine which bacterial components are responsible for this phenomenon. outer membrane (om) extracts were prepared from strains of c. jejuni (3 strains) and c. coli (2 strains). these strains had been isolated from stools of febrile patients with diarrhoea and were able to adhere to hela cells in culture. after incubation of bacterial om extracts with hela c ... | 1989 | 2616891 |
| in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea. | the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ... | 1989 | 2613604 |
| plasmids and serogroups in campylobacter jejuni. | for epidemiological purposes identification of campylobacter strains is usually based on surface antigen characteristics. two different systems, one for heat-stable (hs) and one for heat-labile (hl) antigen have dominated. in earlier studies we found a great variability in the two antigen systems. the aim of the present investigation was to analyse the frequency of plasmids in campylobacter strains in the light of their possible use as an epidemiological tool as well as the relation between the ... | 1989 | 2611024 |
| response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac. | graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ... | 1989 | 2606163 |
| [infectious diarrhea in the adult]. | infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ... | 1989 | 2602888 |
| interaction of campylobacter jejuni with extracellular matrix components. | the adhesion of three strains of campylobacter jejuni to coverslips and microwells coated with isolated extracellular matrix components, fibronectin, laminin and types i, iii, iv and v collagens was studied. fibronectin mediated the adherence of c. jejuni, but there were differences in the binding capacities of the strains. type i, iii and v collagens mediated very strongly the attachment of two strains of c. jejuni. all three strains attached weakly to basement membrane-specific type iv collage ... | 1989 | 2597700 |
| [campylobacter jejuni]. | 1989 | 2595720 | |
| heat resistance of campylobacter and yersinia strains by three methods. | two methods of determining the heat resistance of bacteria, a glass cup and a test tube method, were compared with a method using capillary tubes. three strains of yersinia enterocolitica, one of campylobacter jejuni and two of c. coli were tested in physiological saline. the differences between the results obtained by the glass cup method and the reference method were not statistically significant for five strains and were small also for the other, a yersinia strain. d values obtained by the gl ... | 1989 | 2592291 |
| [campylobacter infection and campylobacter excretion in turkeys]. | five turkey poults at an age of 27 days were infected with campylobacter jejuni strain. additional five poults were infected by contact. turkeys infected by contact and artificially were necropsied 1 and 3 months later, respectively. the histological examination of the liver revealed degenerative changes in contact birds and a proliferation of the connective tissue and bile-ducts in two of the oral infected poults. campylobacter spp. were excreted daily during the first two weeks and afterwards ... | 1989 | 2590155 |
| "campylobacter upsaliensis" isolated from cats as identified by dna relatedness and biochemical features. | campylobacter spp. were isolated from the feces of 20 (58%) of 53 asymptomatic cats during routine physical examination while the cats were maintained in an accredited quarantine facility. fifteen of these campylobacter spp. were identified phenotypically as campylobacter jejuni, and two were identified as c. coli. dna-dna hybridization (hydroxyapatite method) was used to confirm the identification of three thermotolerant catalase-negative isolates. they were 80 to 100% related to each other and ... | 1989 | 2584385 |
| application of different chromosomal dna restriction digest fingerprints to specific and subspecific identification of campylobacter isolates. | eleven strains comprising representatives of different subspecies, biotypes, and serotypes of campylobacter jejuni and reference strains of c. coli, c. fetus subsp. fetus, c. hyointestinalis, and c. sputorum subsp. sputorum were studied to assess the utility of different dna profiles for measuring fine differences between allied bacteria. strains were compared by analyses of haeiii and xhoi digest patterns of chromosomal dna and southern blot hybridization patterns of xhoi digests obtained with ... | 1989 | 2584382 |
| human antibody response to campylobacter jejuni flagellin protein and a synthetic n-terminal flagellin peptide. | we measured isotype-specific human antibodies directed against campylobacter jejuni native flagellin and a synthetic peptide derived from the n-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (elisa). serum samples from patients with gastrointestinal infection caused by c. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. serum s ... | 1989 | 2584372 |
| antigenic heterogeneity of lipopolysaccharides from campylobacter jejuni and campylobacter fetus. | the lipopolysaccharide (lps) structure of campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase k-treated whole cell lysates. polyacrylamide gel electrophoresis lps profiles of c. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant c. fetus strains have smooth-type lps, and serum-sensitive c. fetus strains have rough-type lps. we electroblotted the proteinase k-treated whole cell ... | 1985 | 2580793 |
| western blot analysis of the human antibody response to campylobacter jejuni cellular antigens during gastrointestinal infection. | western blot analysis was used to identify antigenic components of campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. acute- and convalescent-phase sera from eight patients were analyzed for antibody activity against their homologous infecting strains and heterologous clinical isolates. whole-cell and sarkosyl-insoluble membrane components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tra ... | 1985 | 2578479 |
| antigenic analysis of campylobacter flagellar protein and other proteins. | outer membrane proteins of campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to c. jejuni. each campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of dna relat ... | 1985 | 2578478 |
| incidence and sonographic diagnosis of bacterial ileocaecitis masquerading as appendicitis. | 533 consecutive patients with suspected acute appendicitis or appendiceal mass were examined by ultrasonography to distinguish acute appendicitis from bacterial enteritis. in 61 (11.4%) ultrasonography revealed the characteristic picture associated with bacterial enteritis of the ileocaecal region--enlarged mesenteric lymph nodes and mural thickening of the terminal ileum and caecum--but no image of the appendix. in 41 of these a bacterial infection was confirmed: infection due to yersinia enter ... | 1989 | 2567879 |
| campylobacter jejuni infection within a laboratory animal production unit. | a conventional laboratory animal production unit in which rats, mice, guineapigs and rabbits were bred in one building and cats maintained in a separate, but adjacent area was examined for the presence of intestinal thermophilic campylobacter spp. campylobacter jejuni was recovered from 18.84% of 552 animals. the infection rate was highest amongst the cats (51.7%), with rats being the second most commonly infected (23.2%), whereas only 7.7% of guineapigs and a single rabbit (1%) were positive. c ... | 1989 | 2565414 |
| antibacterial effect of the glucose oxidase-glucose system on food-poisoning organisms. | the antibacterial effect of the glucose oxidase-glucose system was studied on food-poisoning organisms including staphylococcus aureus, salmonella infantis, clostridium perfringens, bacillus cereus, campylobacter jejuni, listeria monocytogenes and yersinia enterocolitica using automated turbidometry. the bacteria were grown in sterile-filtered meat medium which was either raw or heat-denaturated. the results showed a clear growth inhibition with combinations of 0.5-1.0 mg/ml glucose and 0.5-1.0 ... | 1989 | 2561954 |
| infection and cross-infection in a paediatric gastro-enteritis unit. | a two month study to investigate the incidence of nosocomial infection was conducted in a paediatric gastroenteritis ward of a black academic hospital. enteric pathogens were identified on admission in 61 (47.2%) of 129 patients; 56 bacterial and 25 viral. six per cent of patients had a combination of bacterial and viral pathogens. enteric pathogens most frequently identified on admission were campylobacter jejuni in 22%. rotavirus in 19.3%, epec in 10.8% and shigella spp. in 6.9% patients. twen ... | 1989 | 2561089 |
| recovery of viruses and bacteria in waters off bondi beach: a pilot study. | a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ... | 1989 | 2556634 |
| [causes of infectious gastro-enteritis in children in dakar]. | between february 1983 and may 1988, 1,157 stools from children aged under 15 years presenting with diarrhoea and admitted to a paediatric hospital in dakar, senegal, were examined for the presence of bacterial and parasitic agents. we looked for campylobacter and rotavirus in only 245 and 111 samples respectively. enterobacteria were detected most frequently (162/264; 61.3 per cent). among these, we found 92 (34.8 per cent) strains of enteropathogenic e. coli; 40 strains (15.1 per cent) of salmo ... | 1989 | 2555813 |
| [gastroenteritis caused by enteric adenovirus serotypes 40 and 41. preliminary study]. | we present the preliminary results of a prospective study of childhood gastroenteritis associated with enteric adenovirus (ae-40 and ae-41). in a period of five months we have studied 499 children with enteritis; 9 (1.8%) were positive for enteric adenoviruses (latex agglutination). the mean age of the children was 16.2 months (extreme ages 10 days and 5 years). the mean duration of diarrhoea was 2.8 days with 4-5 times daily. respiratory symptoms were present in 44.4% of the patients and one pa ... | 1989 | 2552884 |
| nucleotide sequence of a novel kanamycin resistance gene, apha-7, from campylobacter jejuni and comparison to other kanamycin phosphotransferase genes. | a novel kanamycin phosphotransferase gene, apha-7, was cloned from a 14-kb plasmid obtained from a strain of campylobacter jejuni and the nucleotide sequence of the gene was determined. the presumed open reading frame of the apha-7 structural gene was 753 bp in length and encoded a protein of 251 amino acids with a calculated weight of 29,691 da. a 29-kda protein was demonstrated in escherichia coli maxicells containing the cloned apha-7 gene. a ribosomal binding site corresponding to 5 of 8 bas ... | 1989 | 2550983 |
| adhesion to and invasion of hep-2 cells by campylobacter spp. | twenty-one isolates were tested for their ability to adhere to and invade hep-2 cells in vitro. of the 21 organisms tested, 2 did not invade the hep-2 cells, and 1 of these did not adhere to the epithelial cells. campylobacter jejuni clinical isolates were more invasive than the nonclinical strains that were tested. when hep-2 cells were treated with cytochalasin b, the invasiveness of c. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. the n ... | 1989 | 2550368 |
| characterization of the porins of campylobacter jejuni and campylobacter coli and implications for antibiotic susceptibility. | the major outer membrane protein was extracted from campylobacter coli by triton x-100/edta fractionation of cell envelopes. this heat-modifiable protein was shown to have pore-forming activity in black lipid bilayers. the c. coli porin formed a relatively small cation-selective pore with a mean single-channel conductance of 0.53 +/- 0.16 ns in 1.0 m kcl. there was no evidence of oligomer formation, which suggested that each protein monomer formed a pore. pore-forming activity of the c. coli por ... | 1989 | 2543277 |
| the role of radiology in campylobacter enterocolitis. | a series of 18 patients with diarrhoea and positive stool cultures for campylobacter jejuni is presented. the most important radiological features were thickening of ileal mucosal folds, of interhaustral indentations and of the ileocaecal valve, lymphoid hyperplasia and microulcerations. radiology, as well as endoscopy, are both nonspecific in campylobacter jejuni enterocolitis. the importance of radiology is to exclude more typical features of other causes of inflammatory bowel diseases. moreov ... | 1989 | 2541480 |
| [infectious gastroenteritis in the immunocompetent child. significance of cryptosporidium spp. and aeromonas ssp]. | over a one-year period we have examined 390 stool specimens from 210 children hospitalized for infectious gastroenteritis or in whom infectious gastroenteritis occurred during hospitalization. the most prevalent pathogens were rotaviruses (22.4%), followed by enteric salmonella (8.1%), campylobacter jejuni/coli (2.4%), cryptosporidium spp. (2.4%) and aeromonas spp. (1.9%). both cryptosporidium spp. and aeromonas spp. are important pathogens of infectious gastroenteritis in immunocompetent childr ... | 1989 | 2540525 |
| [bacteriological, pharmacokinetic and clinical studies on clarithromycin in the pediatric field. pediatric study group of clarithromycin]. | clarithromycin (te-031, a-56268), a new macrolide antibiotic agent, was evaluated bacteriologically and clinically for its efficacy and safety in pediatrics by a study group organized with pediatricians from all over the country. a summary of the results of the evaluation is as follows. 1. absorption and excretion pharmacokinetics of te-031 was examined by single oral administration of 10% granules and 50 mg tablets at doses of 1, 5, 10 and 15 mg/kg. there were no significant differences between ... | 1989 | 2526259 |
| [clinical study on clarithromycin granule and tablet in the field of pediatrics]. | a newly developed macrolide clarithromycin (te-031, a-56268), with antibacterial spectrum and antibacterial activity nearly equal to those of erythromycin (em), shows beneficial characteristics such as a higher blood level, higher recovery rate in urine, and better penetration into each tissue than conventional macrolides (mls). te-031 has been studied in adults against various infections and proved to be useful. the present paper describes the results of a study in children to examine the usefu ... | 1989 | 2526256 |
| [laboratory and clinical studies on clarithromycin in the field of pediatrics]. | laboratory and clinical studies on clarithromycin (te-031, a-56268), a new macrolide antibiotic, were carried out in the field of pediatrics. the results obtained are summarized as follows: 1. serum concentrations, urinary concentrations and urinary recovery rates were determined upon oral administration on fasting of te-031 at doses of 5 mg/kg granules in 1 case and tablets in 2 cases, and 10 mg/kg granules in 1 and 15 mg/kg granules in 1. peak serum levels were obtained at 30 minutes in 2 case ... | 1989 | 2526245 |
| [pharmacokinetic, bacteriological and clinical studies in the pediatric field on clarithromycin]. | pharmacokinetic, bacteriological and clinical studies were carried out on clarithromycin (te-031, a-56268) in the pediatric field and following results were obtained. 1. peak concentrations of te-031 in the serum occurred at 1-2 hours after administration of 10 mg/kg in granular form and at 2 hours after administration of 20 mg/kg in granular form. half-lives of the drug in serum were 2.5-3.3 hours in cases of 10 mg/kg dosage and 5.5 hour in case of 20 mg/kg dosage. te-031 tablets were administe ... | 1989 | 2526243 |
| [laboratory and clinical studies on clarithromycin in pediatrics]. | clarithromycin (te-031, a-56268) is a new 14-membered ring macrolide antibiotic developed by taisho pharmaceutical co., ltd. te-031 has a methoxy group at position 6 in its structure. in the present study, we carried out laboratory and clinical investigations on te-031 in the field of pediatrics. the obtained results are summarized as follows. the antibacterial activity of te-031 was investigated against 16 clinically isolated strains of streptococcus pyogenes, staphylococcus aureus, haemophilus ... | 1989 | 2526242 |
| [in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics]. | in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ... | 1989 | 2518646 |
| septic abortion due to campylobacter jejuni. | 1989 | 2512152 | |
| rapid method for the detection of dnase of campylobacters. | a rapid agar diffusion method for the detection of dnase production of campylobacter jejuni, c. coli, and c. pylori was developed. a strong pink zone indicating dna hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees c. pretreatment of cells with polymyxin b, which releases the cell-associated dnase, both shortened the time needed to read positive results to 8 h and increased the zone size. | 1989 | 2506224 |
| [epidemiological aspects of enteritis due to campylobacter jejuni]. | from september 1986 through july 1987, all fecal specimens obtained from infants and children who visited the pediatric clinic of the shizuoka general hospital with complaints of abdominal pain or diarrhea were examined for thermophilic campylobacters. bacteriological and epidemiological studies were performed on household contacts. the mothers of the patients were compared with the mothers of age-matched control subjects. bacteriological examination of animals in 49 primary schools in shizuoka ... | 1989 | 2501423 |
| "new" food-borne pathogens of public health significance. | recent work by epidemiologists and microbiologists has uncovered several hitherto unrecognized food-borne bacterial pathogens of public health significance. one of these, listeria monocytogenes, has attracted considerable attention because of two major cheese-related outbreaks of listeriosis that were characterized by cases of meningitis, abortion, and perinatal septicemia. thus far, l. monocytogenes has been responsible for well over 300 reported cases of food-borne listeriosis, including about ... | 1989 | 2501376 |
| [recent bacterial risks in foods]. | knowledge about bacterial foodborne diseases has broadened over the last 15 years. to this development have contributed improved isolation and identification methods but also the application of systematic epidemiological studies. in principle, no "new" bacteria have been discovered but their occurrence in foods was not known (e.g. campylobacter jejuni/coli) or their detection in foods could not be firmly linked with disease in man (e.g. listeria monocytogenes, e. coli o157:h7). for campylobacter ... | 1989 | 2500815 |
| pancreatitis associated with campylobacter jejuni infection: diagnosis by ultrasonography. | 1989 | 2499366 | |
| coumamidines, new broad spectrum antibiotics of the cinodine type. iii. microbiologic activity of coumamidine gamma 1. | the coumamidines are novel antibiotics with activity against a wide spectrum of aerobic gram-positive and gram-negative bacteria. all microbiological studies were performed on coumamidine gamma 1. the mic90s (micrograms/ml) of coumamidine are as follows: staphylococcus aureus 1.0, streptococcus pyogenes 8, enterobacteriaceae 2.0, pseudomonas aeruginosa 8, campylobacter jejuni and campylobacter coli 1, legionella pneumophila 8, haemophilus influenzae 0.5, neisseria gonorrhoeae 0.5. coumamidine ha ... | 1989 | 2498268 |
| in-vitro activity of lomefloxacin in comparison with pefloxacin and ofloxacin. | the in-vitro activity of lomefloxacin (sc 47111, ny-198) was investigated by the determination of mics in agar and in broth, of mbcs in broth, of killing curves and of the duration of the post-antibiotic effect. mics measured in broth and in agar were almost identical. lomefloxacin was two- to eight-fold less active against gram-positive bacteria than ofloxacin. its activity against staphylococcus aureus was independent of resistance to penicillin and oxacillin. the activity of lomefloxacin, ofl ... | 1989 | 2496071 |
| [acute and persistent diarrheal disease and its nutritional consequences in guatemalan infants]. | for the purpose of better understanding the epidemiology of acute and persistent diarrhea, 130 infants of a marginal urban area in guatemala city were studied. the subjects were kept under surveillance by weekly home visits, for periods that varied from three to nine months. the diarrhea episodes were detected and microbiological studies were done in fecal material. additionally, the children were weighed and measured to determine their nutritional status. the infants suffered, on the average, 5 ... | 1989 | 2490881 |
| [isolation of nalidixic acid resistant campylobacter jejuni]. | 1989 | 2490735 | |
| [current problems in the microbiological control of food]. | the paper deals with a number of aspects of current interest and significance on the subject of the microbiological testing of food with particular reference to the role of research institutes. the main objectives of microbiological testing of food are essentially two: (i) to establish the absence of a human health hazard due to microbial contamination of food (food safety); and (ii) to define the quality standard of food (food quality). as far as the first of these objectives is concerned, the ... | 1989 | 2483651 |
| aetiological factors of infantile diarrhoea: a community-based study. | a community-based study was undertaken to compare the organisms responsible for diarrhoea in children living in formal housing with indoor water supply and sanitation with those from a deprived environment. the role of "home remedies" was also assessed. among 373 children with diarrhoea, rotavirus was detected in 15% (in 371 symptom-free controls, 9%), and proved to be the single most common causative agent. bacterial pathogens were found in 20% of patients, with enteropathogenic escherichia col ... | 1989 | 2482008 |
| prospective study of campylobacter jejuni infection in chilean infants evaluated by culture and serology. | a prospective study of campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 chilean infants. surveillance was based on biweekly home visits by a team of trained nurses. c. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic controls and by performing an enzyme-linked immunosorbent assay developed to measure c. jejuni outer membrane protein polyvalent antibodies in a representative group of infants. c. jejun ... | 1989 | 2473090 |
| characterization of cross-reacting serotypes of campylobacter jejuni. | some strains of campylobacter jejuni react with more than one reference antiserum from the serotyping scheme based on heat-stable lipopolysaccharide antigens. to investigate the molecular basis of these cross-reactions, lipopolysaccharides from the reference strains for serotypes 4, 13, 16, 43, and 50 and isolates recovered during two different outbreaks of c. jejuni enteritis were analyzed by passive haemagglutination and sodium dodecyl sulphate-polyacrylamide gel electrophoresis coupled with s ... | 1989 | 2472859 |
| flagellin expression in campylobacter jejuni is regulated at the transcriptional level. | campylobacter jejuni 81116 is able to switch flagellum formation on and off. to study the expression of flagellin, the main component of flagella, an expression library of c. jejuni dna was constructed in lambda gt11. screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it contained a dna insert of 850 base pairs and coded for about 15 kilodaltons of the flagellin, corresponding to 410 base pairs of the flagell ... | 1989 | 2466792 |
| experimental production of proliferative ileitis in syrian hamsters (mesocricetus auratus) by using an ileal homogenate free of campylobacter jejuni. | the role of campylobacter jejuni in the pathogenesis of proliferative ileitis of syrian hamsters (mesocricetus auratus) has been uncertain. c. jejuni has been implicated as the etiologic agent on the basis of the campylobacter-type morphology of the intracellular organism and the repeated microbiologic isolation of c. jejuni from hamsters with proliferative ileitis. the inability to reproduce the disease with pure culture inocula, coupled with immunohistochemical studies, however, has suggested ... | 1989 | 2462537 |
| identification of an antigen common to different species of the genus campylobacter. | affinity-purified antibody specific for a determinant on flagellin from campylobacter jejuni was used to screen by immunoblotting strains of c. coli, c. laridis, c. fetus, "c. upsaliensis," c. pylori, and c. sputorum biovar fecalis. the antigen was detected in each of these species, but the molecular weights of the proteins bearing the common antigen varied considerably. | 1988 | 2457601 |
| bacterial, parasitic and viral enteropathogens associated with diarrhoea in saudi children. | in a 2-year study of stools from saudi children with gastro-enteritis and from controls, rotavirus was the pathogen most frequently detected, either alone (44.3%) or in combination with other enteropathogens (7%). there were two peaks for rotavirus isolates, one during the cold months and the other during the dry, hot season. salmonella spp. and campylobacter jejuni were the second and third most frequently isolated enteropathogens. enteropathogenic escherichia coli did not contribute significan ... | 1988 | 2456718 |
| isolation and characterization of campylobacter flagellins. | sequential acid ph dissociation, differential ultracentrifugation, and neutral ph reassociation were used to partially purify serotypically distinct flagella from three strains of campylobacter jejuni and the two antigenic phases of flagella of campylobacter coli vc167. each c. jejuni flagellin and c. coli vc167 antigenic phase 1 flagellin were purified to homogeneity by reverse-phase high-performance liquid chromatography with a c8 spheri-10 column. c. coli vc167 antigenic phase 2 was purified ... | 1987 | 2444578 |
| antigenic variation of campylobacter flagella. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis of flagella dissociated from strains of campylobacter coli and campylobacter jejuni belonging to the heat-labile serogroup lio 8 showed that some strains were capable of producing flagellin subunits of two different molecular weights (mrs), 59,500 and 61,500. immunoelectron microscopy of cultures of the type strain of this serogroup, c. coli vc167, showed the presence of two flagellum filaments of different antigenic specificity. epitopes ... | 1987 | 2444577 |
| immunochemical approaches to gene probe assays. | antibodies specific for helical nucleic acids can be applied to assays for hybridized dna and/or rna. the assays can use either radioactive or nonradioactive detection systems. antibodies specific for rna-dna hybrids are applicable to assays for measuring hybrid helices that are immobilized on plastic or nitrocellulose, whether the helices are preformed in solution or are formed on the solid-phase support. alternatively, anti-rna-dna hybrid antibodies can be immobilized and used to capture hybri ... | 1987 | 2437829 |
| the influence of immunity on raw milk--associated campylobacter infection. | after a retreat to an oregon farm, 19 of 31 college students developed an acute gastrointestinal illness. campylobacter jejuni infection was recognized in all the ill students and caused asymptomatic infections in three others. in total, 22 (88%) of 25 students who consumed raw milk for the first time became infected as compared with none of two who had not consumed raw milk. among ten persons who chronically consumed raw milk, none was ill, a striking difference from the 76% attack rate among t ... | 1987 | 2431167 |
| location of epitopes on campylobacter jejuni flagella. | flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ... | 1986 | 2430943 |
| common and specific epitopes of campylobacter flagellin recognized by monoclonal antibodies. | murine monoclonal antibodies to campylobacter jejuni recognized a flagellin epitope common to most campylobacter species and an epitope restricted to c. jejuni and c. coli. these epitopes are distinct from the serotype-specific epitope recently detected on the flagellin and have not been described previously. | 1986 | 2426201 |
| isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli. | flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ... | 1986 | 2424932 |
| monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice. | eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ... | 1986 | 2422256 |
| a comparative study of the rod and coccoid forms of campylobacter jejuni atcc 29428. | coccoid forms in cultures of a strain of the enteric pathogen campylobacter jejuni were investigated. a culture containing 100% coccoid forms was non-viable. coccoid forms had a lesser content of cytoplasmic components and nucleic acids than rods of c. jejuni. during the conversion to coccoid forms nucleotides leaked from the cells. the results of treatments with ionic and non-ionic detergents, and lysozyme and ethylenediaminetetraacetic acid indicated a changed cell wall in coccoid forms compar ... | 1986 | 2422149 |
| anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections. | several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ... | 1986 | 2421739 |
| basis for serological heterogeneity of thermostable antigens of campylobacter jejuni. | lipopolysaccharides (lps) were extracted from eight strains of campylobacter jejuni and purified by enzyme treatment to remove traces of rna, dna, and protein. this material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype c. jejuni. the results confirmed that the thermostable antigen typing scheme is based on lps (o) antigens. the lps after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to ... | 1985 | 2412962 |