Publications

[dietary regime and sensitivity of larvae of aedes aegypti to ddt; influence of the lipid level of the organism].		2013	13329775
[report for the month of february 1956 on the aedes aegypti control program in american countries and territories].		1956	13328961
role of diet in egg development by mosquitoes (aedes aegypti).		1956	13324108
a brief note on the occurrence of aedes aegypti in the city of baghdad.		1956	13316289
the comparative morphological and physiological effects of various drugs on the sporogonous cycle of plasmodium gallinaceum in aedes aegypti.		1955	13271507
[report of march, 1955, on the campaign of eradication of aedes aegypti in the countries and territories of the americas].		1955	13260399
[report of the month of january 1955 on the eradication campaign of aedes aegypti in the countries and territories of the americas].		1955	13260397
[campaign of eradication of aedes aegypti in the americas; its organization, development and results since december 1954].		1955	13260395
behaviour differences in two strains of aëdes aegypti.		1955	13244634
studies on the intake of microfilariae by their insect vectors, their survival, and their effect on the survival of their vectors. vii. further observations on the intake of the microfilariae of dirofilaria immitis by aedes aegypti in laboratory conditions: the pattern of the intake of a group of flies.		1955	13239083
[monthly report on the campaign of eradication of aedes aegypti in american countries and territories].		1954	13230222
residual effect of ddt in a controlled area of british guiana tested by the continued release of anopheles darlingi and aedes aegypti: a practical technique for the standardized evaluation of over-all residual efficiency under field conditions.		1954	13216955
the transmission of semliki forest virus by aedes aegypti.		1954	13212871
the yellow fever situation in africa.	while the twentieth century has produced great developments in the epidemiology of yellow fever and in techniques for its control in urban areas, the essential method of control has not changed: it is still the elimination of the urban vector, aëdes aegypti. the aim of those responsible for yellow fever control in africa should be the eradication of this vector from all urban communities in the endemic area. in the case of sylvan yellow fever, complete control of the vectors is not yet possible, ...	1954	13209300
studies on the north american arthropod-borne encephalitides. vii. estimation of amount of eastern equine encephalitis virus inoculated by infected aedes aegypti.		1954	13207100
[monthly report on eradication of aedes aegypti in american countries].		1954	13181996
[technical bases and standards of evaluation of the campaign against aedes aegypti].		1954	13181984
[monthly report on eradication of aedes aegypti in american countries, december, 1953].		1954	13181976
observation on the biology of west nile virus, with special reference to its behaviour in the mosquito aëdes aegypti.		1954	13149118
interaction of insect trypanosomatids with mosquitoes, sand fly and the respective insect cell lines.	interaction experiments between hematophagous insects and monoxenous trypanosomatids have become relevant, once cases of human infection involving these protozoa have been reported. moreover, investigations related to the interaction of insects with trypanosomatids that harbour an endosymbiotic bacterium and thereby lack the paraflagellar rod structure are important to elucidate the role of this structure in the adhesion process. in this work, we compared the interaction of endosymbiont-bearing ...	2003	13129523
healing of gut wounds in the mosquito aedes aegypti (l.) and the leafhopper orosius argentatus (ev.).		1953	13126036
transmission of a west nile-like virus by aedes aegypti.		1953	13090296
studies on the intake of microfilariae by their insect vectors, their survival, and their effect on the survival of their vectors. i. dirofilaria immitis and aëdes aegypti.		1953	13080982
control of malaria in mauritius; eradication of anopheles funestus and aedes aegypti.		1953	13077717
[monthly report on eradication of aedes aegypti in american countries, january, 1953].		1953	13066562
[monthly report on the progress of campaign for eradication of aedes aegypti in american countries, october, 1952].		1953	13051435
the cutaneous reaction to the bite of the mosquito aëdes aegypti (l.) and its alleviation by the topical application of an antihistaminic cream (pyribenzamine).		1953	13044474
[monthly report on the campaign of eradication of aedes aegypti in american countries, september, 1952].		1953	13041772
aedes aegypti surveys in three cities in alabama in 1952.		1953	13023298
the growth stimulating effect of apf, terramycin hydrochloride, vitamin b12 and an undetermined factor x upon aedes aegypti (l) diptera, culicidae.		1953	13007931
[aedes aegypti eradication campaign in chile].		2016	12977855
analysis of the wild-type and mutant genes encoding the enzyme kynurenine monooxygenase of the yellow fever mosquito, aedes aegypti.	kynurenine 3-monooxygenase (kmo) catalyses the hydroxylation of kynurenine to 3-hydroxykynurenine. kmo has a key role in tryptophan catabolism and synthesis of ommochrome pigments in mosquitoes. the gene encoding this enzyme in the yellow fever mosquito, aedes aegypti, is called kynurenine hydroxylase (kh) and a mutant allele that produces white eyes has been designated khw. a number of cdna clones representative of wild-type and mutant genes were isolated. sequence analyses of the wild-type and ...	2003	12974953
insecticide susceptibility status of aedes aegypti to ddt and dieldrin in desert and non-desert parts of rajasthan.	seasonal prevalence and insecticide susceptibility tests were carried out on the adults of aedes aegypti, the vector of dengue and dengue hemorrhagic fever(df/dhf), in three desert (bikaner, jaisalmer and jodhpur) and three non-desert (alwar, ajmer and jaipur) districts of rajasthan. among the three species viz. ae. aegypti, ae. vittatus and ae. w-albus encountered during the survey the former was the most prevalent species. studies were carried out both in rural and urban areas against differen ...	2003	12974421
resistance of aedes aegypti from the state of são paulo, brazil, to organophosphates insecticides.	since the reintroduction of aedes aegypti in the state of s o paulo, in the middle of the 1980-decade, organophosphate insecticides are being used to control the dengue vector. in 1996, an annual program for monitoring the susceptibility of ae. aegypti to the insecticides was implemented. some of the results of this monitoring program are presented. ae. aegypti populations from ten localities have been submitted to bioassays with the diagnostic dose of temephos and fenitrothion. only two (mar li ...	2003	12973541
are vectors able to learn about their hosts? a case study with aedes aegypti mosquitoes.	the way in which vectors distribute themselves amongst their hosts has important epidemiological consequences. while the role played by active host choice is largely unquestioned, current knowledge relates mostly to the innate response of vectors towards stimuli signalling the presence or quality of their hosts. many of those cues, however, can be unpredictable, and therefore prevent the incorporation of the appropriate response into the vector's behavioural repertoire unless some sort of associ ...	2003	12973535
oviposition activity and seasonal pattern of a population of aedes (stegomyia) aegypti (l.) (diptera: culicidae) in subtropical argentina.	monthly oviposition activity and the seasonal density pattern of aedes aegypti were studied using larvitraps and ovitraps during a research carried out by the public health ministry of salta province, in tartagal, aguaray and salvador mazza cities, in subtropical argentina. the a. aegypti population was active in both dry and wet seasons with a peak in march, accordant with the heaviest rainfall. from may to november, the immature population level remained low, but increased in december. ae. aeg ...	2003	12973534
insecticide susceptibility tests of anopheles minimus s.l., aedes aegypti, aedes albopictus, and culex quinquefasciatus in northern thailand.	the susceptibility of anopheles minimus s.l., aedes aegypti, ae. albopictus, and culex quinquefasciatus to insecticide in northern thailand was monitored by using the who standard susceptibility test. one- to two-day old female mosquitos, which were reared from wild caught females or immature stages, were exposed to discriminating dosages of insecticides for recommended exposure periods, and the 24-hour mortality recorded. the results revealed that, in general, an. minimus s.l. was still suscept ...	2003	12971519
aedes aegypti in south vietnam: ecology, genetic structure, vectorial competence and resistance to insecticides.	in vietnam, dengue hemorrhagic fever has been detected since the 1950s. in southeast asia, urban centers expanded rapidly in an uncontrolled and unplanned way. the aedes aegypti populations and dengue viruses thrived in these new ecological and demographic settings. the result of these changes was a greatly extended geographic distribution, increased densities of ae. aegypti and the maintenance of the four dengue serotypes leading to a dramatic increase in dengue transmission. to assess the role ...	2003	12971518
enzootic transmission of yellow fever virus in peru.	the prevailing paradigm of yellow fever virus (yfv) ecology in south america is that of wandering epizootics. the virus is believed to move from place to place in epizootic waves involving monkeys and mosquitoes, rather than persistently circulating within particular locales. after a large outbreak of yfv illness in peru in 1995, we used phylogenetic analyses of virus isolates to reexamine the hypothesis of virus movement. we sequenced a 670-nucleotide fragment of the prm/e gene region from 25 p ...	2003	12967489
introduction of culex toxicity into bacillus thuringiensis cry4ba by protein engineering.	bacillus thuringiensis mosquitocidal toxin cry4ba has no significant natural activity against culex quinquefasciatus or culex pipiens (50% lethal concentrations [lc(50)], >80,000 and >20,000 ng/ml, respectively). we introduced amino acid substitutions in three putative loops of domain ii of cry4ba. the mutant proteins were tested on four different species of mosquitoes, aedes aegypti, anopheles quadrimaculatus, c. quinquefasciatus, and c. pipiens. putative loop 1 and 2 exchanges eliminated activ ...	2003	12957922
diversity of bacillus thuringiensis strains from latin america with insecticidal activity against different mosquito species.	the characterization of selected bacillus thuringiensis strains isolated from different latin america countries is presented. characterization was based on their insecticidal activity against aedes aegypti, culex quinquefasciatus, and anopheles albimanus larvae, scanning electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and plasmid profiles as well as pcr analysis using novel general and specific primers for cry and cyt genes encoding proteins active against mosquit ...	2003	12957913
guidelines on the cost-effectiveness of larval control programs to reduce dengue transmission in puerto rico.	in the past 20 years, the emphasis for avoiding dengue epidemics has focused on larval control of aedes aegypti, the principal mosquito vector of dengue viruses. a general consensus is that mosquito larval control holds the best promise for reducing dengue epidemics, although its actual effectiveness is still unknown and subject to a great deal of uncertainty. the objective of this research was to assess the cost-effectiveness of emergency larval control programs for reducing dengue transmission ...	2003	12952602
aedes aegypti ferritin.	diseases transmitted by hematophagous (blood-feeding) insects are responsible for millions of human deaths worldwide. in hematophagous insects, the blood meal is important for regulating egg maturation. although a high concentration of iron is toxic for most organisms, hematophagous insects seem unaffected by the iron load in a blood meal. one means by which hematophagous insects handle this iron load is, perhaps, by the expression of iron-binding proteins, specifically the iron storage protein ...	2003	12950250
synthesis and repellent efficacy of a new chiral piperidine analog: comparison with deet and bayrepel activity in human-volunteer laboratory assays against aedes aegypti and anopheles stephensi.	optically active (1s,2's)-2-methylpiperidinyl-3-cyclohexen-1-carboxamide (ss220) is a new synthetic arthropod repellent. a three-step synthesis based on a chiral diels-alder reaction and diastereomeric resolution of 2-methylpiperidine was developed to prepare the compound. quantitative laboratory assays using human volunteers compared the effectiveness of ss220 with the commonly used repellents deet and bayrepel against aedes aegypti (linnaeus) and anopheles stephensi liston mosquitoes. in two e ...	2003	12943107
first report of the oriental mosquito aedes albopictus on the west african island of bioko, equatorial guinea.	the invasive oriental mosquito aedes (stegomyia) albopictus (skuse) (diptera: culicidae) was detected on bioko island for the first time in november 2001. it was found to be well established breeding in artificial containers at planta, near malabo, the capital of equatorial guinea. associated species of mosquito larvae were aedes aegypti (l.), ae. africanus (theobald), culex near decens theobald, cx. duttoni theobald, cx. quinquefasciatus say, cx. tigripes de grandpré & de charmoy, eretmapodites ...	2003	12941021
growth characteristics of the veterinary vaccine candidate chimerivax-west nile (wn) virus in aedes and culex mosquitoes.	in 1999 west nile (wn) virus was introduced to north america where this flavivirus has spread rapidly among wildlife (especially birds) transmitted by various species of mosquitoes (diptera: culicidae). increasing numbers of cases and deaths among humans, horses and other domestic animals require development of effective vaccines. 'chimerivax-west nile(vet)' is being developed for use as a veterinary vaccine to protect against wn infection. this chimeric virus contains the pre-membrane (prm) and ...	2003	12941006
larvicidal activity of the essential oil from lippia sidoides cham. against aedes aegypti linn.	the aim of this work was to study the larvicidal activity of lippia sidoides essential oil against aedes aegypti larvae. the essential oil and its hydrolate (saturated solution of essential oil in water) were obtained by vapor extraction and their chemical composition determined by gl-chromatography coupled to mass spectroscopy. bioassays were run with the essential oil, pure and diluted hydrolate and with their main constituents thymol and carvacrol. the results obtained showed that l. sidoides ...	2003	12937776
[larval susceptibility of aedes aegypti and culex quinquefasciatus populations to chemical insecticides].	to evaluate the susceptibility to chemical insecticides of culex quinquefasciatus and aedes aegypt larvae from areas subjected to control treatments or not.	2003	12937715
dengue and the risk of urban yellow fever reintroduction in são paulo state, brazil.	to propose a mathematical method for the estimation of the basic reproduction number, r0, of urban yellow fever in a dengue-infested area.	2003	12937709
utility of comparative anchor-tagged sequences as physical anchors for comparative genome analysis among the culicidae.	the development of comparative genetic maps in multiple species of mosquitoes could prove extremely useful in the search for those genes that contribute to mosquito vector competence or genes associated with other phenotypes of interest. to effectively compare these gene maps, markers must be developed that are based on chromosomal regions conserved throughout the culicidae. we designed 35 polymerase chain reaction (pcr) primer pairs based upon orthlogous exons in aedes aegypti and drosophila me ...	2003	12932106
recombinant american cockroach component, per a 1, reactive to ige of allergic thai patients.	twelve similar recombinant per a 1 clones were produced from an american cockroach (cr) cdna library. the nucleotide sequence of a representative cline, i.e. clone a6, contained 579 base pairs (bp) and a 372 bp open reading frame (2-373) encoding 124 amino acids. a stop codon was found at position 374-376 followed by a 3' end untranslated region with an aataaa polyadenylation signal and a poly (a) tail. the estimated molecular mass of the 24 amino acid residue protein was 13.8 kda, with a predic ...	2003	12931746
regulation of tight junction permeability with switch-like speed.	the case is made that tight junctions can undergo large reversible conductance changes in a matter of seconds and yet preserve their permselectivity. the diuretic peptide leucokinin transforms (renal) malpighian tubules of the yellow fever mosquito from a moderately tight epithelium to a leaky epithelium by increasing the chloride-conductance of the paracellular shunt pathway. the nine-fold increase in the paracellular chloride-conductance brings about a non-selective stimulation of transepithel ...	2003	12920403
the histone-like c-terminal extension in ribosomal protein s6 in aedes and anopheles mosquitoes is encoded within the distal portion of exon 3.	in eukaryotic cells, ribosomal protein s6 (rps6) is the major phosphorylated protein on the small ribosomal subunit. in the mosquitoes aedes aegypti and aedes albopictus, the cdna encoding rps6 contains 300 additional nucleotides, relative to the drosophila homolog. the additional sequence encodes a 100-amino acid, lysine-rich c-terminal extension of the rps6 protein with 42-49% identity to histone h1 proteins from the chicken and other multicellular organisms. using mass spectrometry we now sho ...	2003	12915181
post-integration behavior of a mos1 mariner gene vector in aedes aegypti.	the post-integration behavior of insect gene vectors will determine the types of applications for which they can be used. transposon mutagenesis, enhancer trapping, and the use of transposable elements as genetic drive systems in insects requires transposable elements with high rates of remobilization in the presence of transposase. we investigated the post-integration behavior of the mos1 mariner element in transgenic aedes aegypti by examining both germ-line and somatic transpositions of a non ...	2003	12915177
the dynamics of interactions between plasmodium and the mosquito: a study of the infectivity of plasmodium berghei and plasmodium gallinaceum, and their transmission by anopheles stephensi, anopheles gambiae and aedes aegypti.	knowledge of parasite-mosquito interactions is essential to develop strategies that will reduce malaria transmission through the mosquito vector. in this study we investigated the development of two model malaria parasites, plasmodium berghei and plasmodium gallinaceum, in three mosquito species anopheles stephensi, anopheles gambiae and aedes aegypti. new methods to study gamete production in vivo in combination with gfp-expressing ookinetes were employed to measure the large losses incurred by ...	2003	12906877
rapid loss of genetically based resistance to metals after the cleanup of a superfund site.	over the period 1953-1979, a battery factory on the hudson river in new york released approximately 53 tons of cadmium (cd) and nickel hydride wastes into foundry cove. the most common aquatic benthic species, the oligochaete limnodrilus hoffmeisteri, rapidly evolved resistance to cd. the capacity for detoxification and internal storage of cd resulted in a strong potential for trophic transfer of cd through the aquatic food web. as a result of united states superfund legislation, a major remedia ...	2003	12904581
improved molecular detection of dengue virus serotype 1 variants.	the dengue virus molecular typing method described by lanciotti and coworkers (r. s. lanciotti, c. h. calisher, d. j. gubler, g. j. chang, and a. vance-vorndam, j. clin. microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. however, it failed to detect denv-1 variants in cambodia due to a point mutation. we describe an improvement of the method that allows the detection of additional denv-1 strains, including potential variants.	2003	12904404
a technique for preparing polytene chromosomes from aedes aegypti (diptera, culicinae).	polytene chromosome preparations were obtained from larval, pupal and adult female malpighian tubules of aedes aegypti. the malpighian tubules of the pupae (0-4 h old) from larvae reared at 20 c provided the best cytogenetic analysis. the interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic ac ...	2003	12886421
deletions in the putative cell receptor-binding domain of sindbis virus strain mre16 e2 glycoprotein reduce midgut infectivity in aedes aegypti.	the sindbis virus (alphavirus; togaviridae) strain mre16 efficiently infects aedes aegypti mosquitoes that ingest a blood meal containing 8 to 9 log(10) pfu of virus/ml. however, a small-plaque variant of this virus, mre16sp, poorly infects mosquitoes after oral infection with an equivalent titer. to determine the genetic differences between mre16 and mre16sp viruses, we have sequenced the mre16sp structural genes and found a 90-nucleotide deletion in the e2 glycoprotein that spans the 3' end of ...	2003	12885905
benzoquinones from millipedes deter mosquitoes and elicit self-anointing in capuchin monkeys (cebus spp.).	neotropical monkeys of the genus cebus anoint themselves by rubbing arthropods and plants against their pelage. a recent study has shown that free-ranging wedge-capped capuchin monkeys (c. olivaceus) in venezuela self-anoint with a benzoquinone-secreting millipede, an activity by which they are hypothesized to appropriate chemical deterrents of mosquitoes. to evaluate the plausibility of this hypothesis, female yellow fever mosquitoes (aedes aegypti) were presented with two millipede secretory c ...	2003	12883771
identification, functional characterization and expression of a lat type amino acid transporter from the mosquito aedes aegypti.	we isolated two cdnas from the mosquito aedes aegypti, an l-amino acid transporter (aealat) and a cd98 heavy chain (aeacd98hc). expression of aeacd98hc or aealat alone in xenopus oocyte did not induce amino acid transport activity. however, co-expression of aeacd98hc and aealat, which are postulated to form a heterodimer protein linked through a disulfide bond, showed significant increase in amino acid transport activity. this heterodimeric protein showed uptake specificity for large neutral and ...	2003	12878228
wax moth, galleria mellonella, high density lipophorin receptor: alternative splicing, tissue-specific expression, and developmental regulation.	a lipophorin (lp) receptor cdna from the fat body of galleria mellonella (lepidoptera) was cloned and sequenced. this is the first result in this order, lepidoptera. it showed the pattern of the vldl receptor belonging to the ldl receptor family. sequence homology with other lp receptors in insects, locusta migratoria and aedes aegypti, was 70 and 61%, respectively and each domain was highly conserved. polyclonal anti-lp receptor antibody prepared against expressed lp receptor fragment between l ...	2003	12878223
identification of the people from whom engorged aedes aegypti took blood meals in florida, puerto rico, using polymerase chain reaction-based dna profiling.	we used polymerase chain reaction-based dna profiling to construct allelic profiles for residents and visitors of 22 houses in florida, puerto rico, and human dna from blood meals in aedes aegypti that were collected in those homes. complete profiles were obtained for < or = 2 days after blood ingestion. eighteen percent of the meals came from two different people. there was no evidence of meals from > or = 2 people. eighty percent of the meal sources were identified, > 70% were taken from resid ...	2003	12875293
kinetics of cellular responses to intraperitoneal brugia pahangi infections in normal and immunodeficient mice.	filarial infections evoke exuberant inflammatory responses in the peritoneal cavities of immunocompetent mice. clearance of infection appears to be dependent on complex interactions between b1 and b2 b lymphocytes, t cells, eosinophils, macrophages, and the products of these cells. in an earlier communication, we described the course of infection in normal immunocompetent mice. in this study, we utilize mice with well-characterized mutations that disable one or more effector components of adapti ...	2003	12874313
efficacy of two organophosphorus insecticides, reldan & dursban against the larvae of culex quinquefasciatus.	the resistance in vectors to conventional insecticides warrants development of newer insecticides for mosquito control besides use of other counter-measures. the present study was undertaken to test the efficacy of a new and toxicologically safe organophosphorus compound, reldan (chlorpyrifos-methyl) and compare its efficacy with dursban (chlorphyrifos-ethyl) against larvae of different mosquito vectors under laboratory conditions and against culex quinquefasciatus in three larval habitats.	2003	12866823
expression of an aedes aegypti cation-chloride cotransporter and its drosophila homologues.	insects maintain haemolymph homeostasis under different environmental conditions by modulating the concentrations of na+, k+ and cl- ions. one group of proteins involved in ion transport across cell membranes consists of cation-chloride cotransporters that form a family of structurally similar proteins. although much is known about these proteins in mammalian systems, our understanding of them in insects is lacking. the recent sequencing of two insect genomes, drosophila and anopheles, enabled u ...	2003	12864912
comparison of the mosquito-repelling efficacy of methyl neodecanamide (mnda) to that of deet.	the objective of this small pilot study was to assess the mosquito-repelling efficacy of methyl neodecanamide (mnda) relative to n,n-diethyl-3-methylbenzamide (deet) against anopheles stephensi, culex quinquefasciatus, and aedes aegypti under controlled laboratory conditions. in this study, subjects inserted both forearms (sham- and repellent-treated) into a test chamber containing 50 female mosquitoes. the number of mosquitoes landing on or probing each forearm during the 5-minute "forced-choic ...	2006	12858227
the structural determination of an insect sterol carrier protein-2 with a ligand-bound c16 fatty acid at 1.35-a resolution.	yellow fever mosquito sterol carrier protein (scp-2) is known to bind to cholesterol. we report here the three-dimensional structure of the complex of scp-2 from aedes aegypti with a c16 fatty acid to 1.35-a resolution. the protein fold is exceedingly similar to the human and rabbit proteins, which consist of a five-stranded beta-sheet that exhibits strand order 3-2-1-4-5 with an accompanying layer of four alpha-helices that cover the beta-sheet. a large cavity exists at the interface of the lay ...	2003	12855689
comparison of the effect of two excipients (karite nut butter and vaseline) on the efficacy of cocos nucifera, elaeis guineensis and carapa procera oil-based repellents formulations against mosquitoes biting in ivory coast.	repellents in the form of dermal pomades are recommended as a protection against awakening and bedtime mosquito bites. if synthesis repellents are available, they are nevertheless not common and the prices remain out of reach for the communities concerned. the people therefore have to resort more and more to traditional concoctions, some of which have been shown to be effective. after demonstrating that oil-based formulations (lotions, creams, pomades) of cocos nucifera (coconut), elaeis guineen ...	2003	12847928
population and parity levels of aedes aegypti collected in tucson.	oviposition traps were used to follow changes in the population of aedes aegypti (l.) (diptera:culicidae) in a seven-block area in midtown region of tucson, arizona. about 20,000 eggs were collected over a period from 1 june to 14 october 2000. peak mosquito populations were correlated with the late summer rains. mosquitoes seeking a blood meal were collected and dissected to determine if they had previously fed, i.e. if they were parous. of the 241 females examined, 44% were parous, with a rang ...	2003	12831130
bromeliad-inhabiting mosquitoes in south florida: native and exotic plants differ in species composition.	at several locations in south florida, water impounded by the leaf axils of native or exotic bromeliads was sampled for immature mosquitoes. culex biscaynensis was the most commonly collected mosquito from exotic bromeliads at study sites in southeastern miami-dade county, whereas at nearby sites with native bromeliads, immature wyeomyia mitchellii were more abundant than immature culex biscaynensis. aquatic habitat size and persistence may be important factors favoring culex biscaynensis in exo ...	2003	12831127
physiological bases of mosquito ecology.	the research carried out during more than 30 years in the author's laboratory is briefly reviewed. quantitative analyses of basic physiological processes, such as growth and development, digestion and excretion, oogenesis and fecundity, reserve synthesis and resulting flight-potentials of aedes aegypti were summarized and compared with several other mosquito species, particularly with anopheles. these studies led to the recognition of distinctly different physiological strategies, for which the ...	2003	12831123
climatic and social risk factors for aedes infestation in rural thailand.	an intense epidemic of dengue haemorrhagic fever in 1998 prompted the thai government to investigate the feasibility of focalized vector (aedes aegypti) control programmes. we tested for correlations of three indices of aedes larval abundance (housing index, container index and breteau index) against 38 socio-economic and four climatic variables. availability of public water wells, existence of transport services and proportion of tin houses were positively associated with larval indices. privat ...	2003	12828549
does s-methoprene affect oviposition by aedes aegypti in an ovitrap?	we conducted a field study to test if the addition of a pellet of s-methoprene (altosid pellets) to an ovitrap would affect oviposition by aedes aegypti or ochlerotatus notoscriptus. in 2 trials conducted near cairns, queensland, australia, no significant difference (p > 0.05) was found in the number of eggs of ae. aegypti or oc. notoscriptus laid in ovitraps with or without a methoprene pellet. these results suggest the addition of an s-methoprene pellet to ovitraps or sentinel tires could be e ...	2003	12825672
evaluation of selected traps as tools for conducting surveillance for adult aedes aegypti in thailand.	the efficacy of the omnidirectional fay-prince trap (odfp), the centers for disease control wilton trap (wt), and sticky lures (sl) were assessed as a means of evaluating population densities of adult aedes aegypti in thailand. human landing/biting (l/b) collection was used as the reference method. the l/b collection method was significantly more effective at capturing ae. aegypti than any of the other methods, with 84% (1,072/1,272), 8.5% (108/1,272), 7.2% (92/1,272) and 0% (0/1,272) of mosquit ...	2003	12825666
field evaluation of a lethal ovitrap against dengue vectors in brazil.	field evaluation of a "lethal ovitrap" (lo) to control dengue vector aedes mosquitoes (diptera: culicidae), was undertaken in two brazilian municipalities, areia branca and nilopolis, in the state of rio de janeiro. the lo is designed to kill aedes via an insecticide-treated ovistrip (impregnated with deltamethrin). in each municipality, the intervention was applied to a group of 30 houses (10 los/house) and compared to 30 houses without los in the same neighbourhood. five los were put outside a ...	2003	12823838
natural skip oviposition of the mosquito aedes aegypti indicated by codominant genetic markers.	this study examines the use of codominant restriction fragment length polymorphism (rflp) markers to estimate the number of sibling families found within and among oviposition sites used by the mosquito aedes aegypti (l) (diptera: culicidae). estimates were made using pairwise relatedness (rxy) calculations based on alleles shared between individuals. genotypes for eight laboratory mosquito families were determined at six rflp loci and the observed allele frequencies were used to generate simula ...	2003	12823837
sublethal iridovirus disease of the mosquito aedes aegypti is due to viral replication not cytotoxicity.	invertebrate iridescent viruses (iridoviridae) possess a highly cytotoxic protein. in mosquitoes (diptera: culicidae), invertebrate iridescent virus 6 (iiv-6) usually causes covert (inapparent) infection that reduces fitness. to determine whether sublethal effects of iiv-6 are principally due to cytotoxicity of the viral inoculum (which inhibits macromolecular synthesis in the host), or caused by replication of the virus larvae of the mosquito aedes aegypti (l) were exposed to untreated iiv-6 vi ...	2003	12823836
mosquito larval consumption of toxic arborescent leaf-litter, and its biocontrol potential.	previously we described the mosquito larvicidal properties of decomposed leaf-litter from deciduous trees, especially the alder alnus glutinosa (l) gaertn., due to toxic polyphenols and other secondary compounds. to further examine the biocontrol potential of toxic leaf-litter for mosquito control, feeding rates of third-instar mosquito larvae were assessed for examples of three genera: anopheles stephensi liston, aedes aegypti (l) and culex pipiens l. (diptera: culicidae). when immersed in a su ...	2003	12823832
thioredoxin from brugia malayi: defining a 16-kilodalton class of thioredoxins from nematodes.	thioredoxins are a family of small redox proteins that undergo nadph-dependent reduction by thioredoxin reductase. this results in a supply of reducing equivalents that cells use in a wide variety of biological reactions, which include maintaining reduced forms of the enzymes important for protection against damage from high-energy oxygen radicals, the regulation of transcription factor activity, and the inhibition of apoptosis. here we report on a new member of the thioredoxin family of protein ...	2003	12819103
efficiency of dengue serotype 2 virus strains to infect and disseminate in aedes aegypti.	dengue serotype 2 (den-2) viruses with the potential to cause dengue hemorrhagic fever have been shown to belong to the southeast (se) asian genotype. these viruses appear to be rapidly displacing the american genotype of den-2 in the western hemisphere. to determine whether distinct genotypes of den-2 virus are better adapted to mosquito transmission, we classified 15 viral strains of den-2 phylogenetically and compared their ability to infect and disseminate in different populations of aedes a ...	2003	12812340
dynamics of molecular markers linked to the resistance loci in a mosquito-plasmodium system.	models on the evolution of resistance to parasitism generally assume fitness tradeoffs between the costs of being parasitized and the costs associated with resistance. this study tested this assumption using the yellow fever mosquito aedes aegypti and malaria parasite plasmodium gallinaceum system. experimental mosquito populations were created by mixing susceptible and resistant strains in equal proportions, and then the dynamics of markers linked to loci for plasmodium resistance and other unl ...	2003	12807772
proline can be utilized as an energy substrate during flight of aedes aegypti females.	in order to determine whether proline can be utilized as fuel during flight of aedes aegypti, proline, alanine, and glutamine concentrations were monitored at 0, 30 and 60 min after flight using sugar-fed males and females, and blood meal-fed females. in sugar-fed and blood meal-fed females, flight lead to a significant decrease in proline and a significant increase in glutamine concentration in both hemolymph and thorax. only during flight after a blood meal was a significant increase in the al ...	2003	12804719
insecticidal activity and chemical composition of volatile oils from hyptis martiusii benth.	the essential oils from leaves and inflorescences of hyptis martiusii benth were analyzed by gc-ms. twenty-six compounds representing 93.2% of the essential oil of leaves were characterized; delta-3-carene (22.5%), 1,8-cineole (24.27%), beta-caryophyllene (6.15%), and bicyclogermacrene (6.32%) were found as the major components. in the essential oil of inflorescences 27 compounds representing 87.7% of the oil were identified. the major components were delta-3-carene (13.5%), alpha-pinene (5.78%) ...	2003	12797740
plagues and peoples revisited. basic and strategic research for ingectious disease control at the intergace in the life, health and social sciences.		0	12789403
ex vivo cytotoxicity of the bacillus thuringiensis cry4b delta-endotoxin to isolated midguts of aedes aegypti larvae.	the pathological effect of the bacillus thuringiensis cry delta- endotoxins on susceptible insect larvae had extensive damage on the midgut epithelial cells. in this study, an ex vivo assay was devised for assessing the insecticidal potency of the cloned cry4b mosquito-larvicidal protein that is expressed in escherichia coli. determination of toxicity was carried out by using a cell viability assay on the midguts that were dissected from 5-day old aedes aegypti mosquito larvae. after incubation ...	2003	12787485
dendritic-cell-specific icam3-grabbing non-integrin is essential for the productive infection of human dendritic cells by mosquito-cell-derived dengue viruses.	dengue virus (dv) is a mosquito-borne flavivirus that causes haemorrhagic fever in humans. dv primarily targets immature dendritic cells (dcs) after a bite by an infected mosquito vector. here, we analysed the interactions between dv and human-monocyte-derived dcs at the level of virus entry. we show that the dc-specific icam3-grabbing non-integrin (dc-sign) molecule, a cell-surface, mannose-specific, c-type lectin, binds mosquito-cell-derived dvs and allows viral replication. conclusive evidenc ...	2003	12783086
laboratory surveillance of dengue in argentina, 1995-2001.	local transmission of dengue fever virus in argentina is increased by the presence of aedes aegypti mosquitoes and dengue outbreaks in neighboring countries. from 1995 to 2001, a laboratory-based active surveillance program detected 922 dengue cases. indigenous transmission involving dengue-1 and -2 serotypes was confirmed only in subtropical areas in northern argentina.	2003	12781019
functional characterization of a glutamate/aspartate transporter from the mosquito aedes aegypti.	glutamate elicits a variety of effects in insects, including inhibitory and excitatory signals at both neuromuscular junctions and brain. insect glutamatergic neurotransmission has been studied in great depth especially from the standpoint of the receptor-mediated effects, but the molecular mechanisms involved in the termination of the numerous glutamatergic signals have only recently begun to receive attention. in vertebrates, glutamatergic signals are terminated by na(+)/k(+)-dependent high-af ...	2003	12771173
the v-type h(+)-atpase in malpighian tubules of aedes aegypti: localization and activity.	the v-type h(+)-atpase is thought to provide the driving force for transepithelial electrolyte and fluid secretion in malpighian tubules. to confirm the presence of this proton pump in malpighian tubules of the yellow fever mosquito aedes aegypti, we used several antibodies raised against the v-type h(+)-atpase of manduca sexta. western blot analysis confirmed the presence of the v-type h(+)-atpase in malpighian tubules of aedes aegypti. in situ immunostaining identified the v-type h(+)-atpase a ...	2003	12771170
natriuretic and depolarizing effects of a stable fly (stomoxys calcitrans) factor on malpighian tubules.	a two-step hplc purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (v(t)) of the adult stable fly malpighian tubule. when applied to tubules of the female mosquito, aedes aegypti, this factor partially mimics the electrophysiological actions of the mosquito natriuretic factor (mnf). it also selectively increases active transepithelial na transport by the mosquito malpighian tubule. the blood meal causes a transient increase ...	1997	12770470
effects of putative diuretic factors on intracellular second messenger levels in the malpighian tubules of aedes aegypti.	intracellular levels of the second messengers, 3',5'-cyclic adenosine monophosphate (camp) and inositol 1,4,5-trisphosphate (ip(3)) were measured in the malpighian tubules of aedes aegypti following the in vitro application of 5-hydroxytryptamine (5-ht) and the putative mosquito diuretic peptides, culex salinarius diuresin and mosquito leucokinins (culekinin depolarizing peptides (cdps) i, ii, iii, a. aegypti leucokinin peptides (alps) i, ii, iii). the c. salinarius diuresin significantly (p<0.0 ...	1999	12770358
the effect of putative diuretic factors on in vivo urine production in the mosquito, aedes aegypti.	changes in total urine production were measured in the mosquito, aedes aegypti, following injection of 5-hydroxytryptamine (5-ht), culex salinarius diuresin, culekinin depolarizing peptides (cdp-i, ii and iii) or the a. aegypti leucokinin peptides (alp-i, ii and iii). all stimulated total urine production in a dose dependent manner. 5-ht was the least potent in urine production experiments with ed(50) values nearly 100-fold higher than other diuretic agonists. doses greater than 2x10(-4) &mgr;mo ...	1999	12770357
sensitivity of the mosquito aedes aegypti (culicidae) labral apical chemoreceptors to phagostimulants.	the feeding of aedes aegypti (l.) on blood and nectar is induced by phagostimulants: adenine nucleotides and sugars respectively. this work examines the responses of the four chemoreceptor cells in the labral apical sensilla to these phagostimulants. the apical chemoreceptors can detect the presence of adenine nucleotides. this part of the response is in good agreement with the gorging behavior. the output of the chemoreceptors cannot distinguish among different adenine nucleotides or among thei ...	1999	12770348
a molecular view of trypsin synthesis in the midgut of aedes aegypti.	ingestion of a blood meal induces two phases of trypsin synthesis in the midgut of aedes aegypti females. the first phase, which encompasses the first 4-6 hours following a blood meal, is characterized by the presence of small amounts of early trypsin. the second phase, which occurs between 8 and 36 hours after blood feeding, is characterized by the presence of large amounts of late trypsin. a specific form of regulation of trypsin synthesis characterizes each phase: early trypsin synthesis is r ...	1999	12770346
sensitivity of the mosquito aedes aegypti (culicidae) labral apical chemoreceptors to blood plasma components.	the phagostimulants from the cellular fraction of blood induce gorging of aedes aegypti (l.), and this process is enhanced by some plasma components. this project examines the responses of the labral apical chemoreceptors to plasma components enhancing phagostimulation. from the electrophysiological responses of the labral apical chemoreceptors four cells were identified by the waveform of their action potentials. three of the cells (cell 2, cell 3 and cell 4) responded in a dose dependent manne ...	1999	12770332
larval growth and biosynthesis of reserves in mosquitoes.	developmental instars of four species of mosquitoes have been analyzed for growth and synthesis of biomass with respect to their caloric content of protein, lipids, and carbohydrates for each instar of aedes aegypti and culex pipiens of the subfamily culicinae, and anopheles albimanus, and an. gambiae of the subfamily anophelinae. the diameter of the thorax grows during the intermolt, reflecting continuous increase in biomass because it correlates significantly with the larval synthesis of total ...	1999	12770329
localization of the mosquito insulin receptor homolog (mir) in reproducing yellow fever mosquitoes (aedes aegypti).	the female mosquito takes a blood meal to produce a batch of eggs. initiation of egg maturation and growth of oocytes is governed by several endocrine factors. peptide factors from the brain are involved in this process and some are also responsible for the induction of ecdysone secretion. the latter appears to be required to maintain a high rate of vitellogenin synthesis. by analogy with the known functions of insulin-like molecules (e.g. bombyxins) which in insects activate the secretion of ec ...	1998	12770311
factors modulating the blood feeding behavior and the electrophysiological responses of labral apical chemoreceptors to adenine nucleotides in the mosquito aedes aegypti (culicidae).	the feeding of aedes aegypti (l.) on blood is induced by the presence of phagostimulants: adenine nucleotides. three chemoreceptive cells in the labral apical sensilla can distinguish the presence of adenine nucleotides depending on the other stimulus components. this work aims at correlating the sensory information arising from the labral apical sensilla with the feeding behavior in response to the same stimuli. the saline stimulating solution, containing adenine nucleotides, is modulated by ch ...	1999	12770292
adaptation of aedes aegypti (diptera: culicidae) oviposition behavior in response to humidity and diet.	effects of humidity and sugar concentration on the fecundity, temporal oviposition patterns and survival of a tropical strain of aedes aegypti (l.) were investigated. fecundity was significantly reduced by low humidity, but was not affected by sugar concentration. low humidity caused a significant decrease in percentage survival after 19 days as compared to high humidity. oviposition was inhibited by host availability for eight successive days. when access to a host was no longer provided, ovipo ...	1999	12770289