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the prevalence of toxoplasma gondii antibodies in man in plateau state and meat animals in nigeria.toxoplasma gondii antibodies were found in the sera of 22.86% of people in the jos area of plateau state, nigeria. the incidence of antibodies in nigerian food animals was 17.40%, the highest being in sheep (21.92%) and lowest in goats (13.88%). cattle and horses were roughly equally infected--about 17.0%. the zoonotic potential of t. gondii in food animals is stressed and epidemiological factors are reviewed.19853992640
[the improvement of solubility behavior of problem drugs. 2. solubility behavior of slow dissolving products of iomeglamic acid and succinic acid]. 20143991791
photochemical decomposition of phenazone derivatives. part 5(1): isolation and identification of decomposition products in aqueous solution.from aqueous solutions of phenazone, aminophenazone, propyphenazone, nifenazone, morazone, noramidopyrine, 4-aminophenazone and isopropylaminophenazone, uv irradiated at lambda = 254 nm in oxygen free atmosphere as well as in the presence of air, 24 decomposition products were isolated and identified as derivatives of pyrazoline, pyrazolidinone, imidazoline, hydantoin, aziridine, phenylhydrazine and aniline.20143991782
[surgically treated cases of childhood and adolescent renovascular hypertension]. 20133991150
effects of rapid cooling on mechanical and electrical responses in ventricular muscle of guinea-pig.the effect of rapidly lowering bathing solution temperature from 36.5 +/- 0.5 degrees c to various low temperatures was examined in guinea-pig ventricular muscle to explore the possible role of intracellular ca2+ store sites in excitation-contraction coupling. rapid cooling from 36.5 +/- 0.5 degrees c to below 18 degrees c caused contracture (rapid cooling contracture, r.c.c.) with subthreshold depolarization for contraction, if electrical stimulation was applied before cooling. r.c.c. peak tens ...19853989731
size, form and orientation of the neuronal perikarya and nuclei in the nucleus infundibularis of the male rat.size, form and orientation of nerve cells of the nucleus infundibularis have been analyzed in frontal, horizontal and sagittal semithin sections of vestopal-embedded hypothalami of the male rat. morphometric studies were performed with a semiautomatic image analyzing system. by combining data from three different planes of section the three-dimensional size, shape and orientation of perikarya and nuclei in the different parts of the nucleus infundibularis as well as gradients in mediolateral, ro ...19853989281
can the rapid semiquantitative estimation of serum c reactive protein be adapted for the management of bacterial infection?serum c reactive protein concentrations measured by a laboratory based assay were compared with the semiquantitative results obtained with a visual agglutination method (well-cotest, crp kit). using this agglutination kit, diluting serum 1/10 and 1/20 gave c reactive protein results which could be of more clinical value than those obtained using the 1/2, 1/4, 1/8, and 1/16 dilutions recommended by the manufacturers. the kit was also used on the ward by junior medical staff, who showed that after ...20143988960
carbohydrate metabolism in transforming lymphocytes from the aged.there is an age-related decline in immune capacity which has been linked to a decreased response of lymphocytes to mitogens in vitro. during transformation, lymphocytes require a marked increase in energy production and biosynthesis which is supplied primarily by glycolysis. in the elderly, the glycolytic enzymes increase significantly in transforming lymphocytes at least 24 hr later than in the young and then at significantly reduced levels. glucose utilization is also impaired in stimulated ly ...20143988814
clinicians' knowledge about the families of their patients.this study was designed to determine what doctors and nurses in family medicine actually know about the families of their patients; to assess the accuracy of the professed knowledge; and to relate this knowledge to the patient's level of satisfaction and compliance. clinicians completed questionnaires dealing with their knowledge of personal and family information about patients. these patients completed a mirror-image questionnaire--to assess the accuracy of the clinician's responses--and a que ...20143988012
complement and hla. further definition of high-risk haplotypes in insulin-dependent diabetes.the families of 41 probands with type i (insulin-dependent) diabetes mellitus (iddm) were typed for hla-a, hla-b, and hla-dr antigens in addition to the complement polymorphisms c2, c4a, c4b, and bf. all of these loci are encoded on the short arm of human chromosome 6 in a narrow region. alleles at hla-b (8, 15, 18, and 40), hla-dr (3 and 4), and bf (f1) have been associated with increased relative risk (rr) for iddm, while hla-b7 and hla-dr2 have been associated with decreased rr for iddm. this ...19853987976
purification of the 25-kda vibrio cholerae major outer-membrane protein and the molecular cloning of its gene: ompv.the 25-kda peptidoglycan-associated outer-membrane protein and most likely porin of vibrio cholerae is a major immunogenic species. it has been purified by ion-exchange elution on hydroxyapatite followed by gel filtration on bio-gel p150 both in the presence of sodium dodecyl sulfate. this protein, of greater than 90% purity as judged by western blotting, has been used to raise antibodies in rabbits. the antisera were then used to screen v. cholerae gene banks, constructed in escherichia coli k1 ...19853987695
cone photoreceptor shedding in the tree shrew (tupaia belangerii).tree shrews were sacrificed at various times during a 12 h light-12 h dark cycle and the retinal pigment epithelium (rpe) was examined for phagosomes. analysis of photoreceptor densities showed that the tree-shrew retina consists of approximately 96% cone photoreceptors. therefore, phagosomes in the rpe were assumed to be mostly those of cones. a peak in the number of rpe phagosomes was found about one hour after the onset of light. the number of phagosomes/mm rpe during the light cycle varied f ...20143986885
effects of peripheral vasoconstriction on the measurement of blood pressure in a finger.using noninvasive techniques only, the fall in mean pressure and the pulse amplification between brachial and finger arterial pressure were measured in six anaesthetised female subjects during surgery. brachial pressure was measured every 2 min with an oscillometric technique. finger pressure was measured continuously using an arterial volume clamp method. in addition changes in the degree of peripheral vasoconstriction were established on an adjacent finger with a photo reflection plethysmograp ...20143986857
a study of the extent and pathology of experimental avulsion injury in rabbit arteries and veins.a comparison was made between operating microscope observations and histopathological examination of the ruptured ends of experimentally avulsed rabbit femoral arteries and veins. under the operating microscope no damage was evident in arteries or veins more than 0.8 cm (on average) from the rupture site, the common lesions being tears, holes, bruising, sleeving and dilatations. in light microscope and electron microscope studies arterial and venous lesions were often noted up to 4 cm from the r ...19853986426
molecular mechanisms of olfactory reception. vi. kinetic characteristics of camphor interaction with binding sites of rat olfactory epithelium.camphor binding to a possible receptor of rat olfactory epithelium has been studied within the ligand concentration range 10(-11)-10(-6) m. at these concentrations camphor is bound by a set of receptors. they are distinguished by both the affinity to the ligand (k1 = 5 x 10(-10) m, k2 = 3.5 x 10(-8) m, k3 approximately equal to 10(-6) m) and their amount in the epithelium. the differences in the affinities are due to different values of the association rate constant of camphor (k1), which varies ...19853986222
exclusive activation of aromatic amines in the marine mussel mytilus edulis by fad-containing monooxygenase.microsomes from the marine mussel mytilus edulis possess the enzyme activity that selectively metabolizes primary aromatic amines and not polycyclic aromatic hydrocarbons. this activity is nadph-dependent and has a ph optimum at 8.4. by these characteristics this enzyme is identical with the purified pig liver fad-containing monooxygenase (ec 1.14.13.8, dimethylaniline monooxygenase). the exposure of mussels to diesel-2 oil does not induce the enzyme activity. these results are discussed in term ...19853985956
[reciprocal regulation of growth and differentiation in hepatocytes by cell surface modulator and loss of regulation during carcinogenesis].in primary monolayer cultures of mature rat hepatocytes, cell growth and hepatocyte-specific functions were mediated by a cell surface component (named the cell surface modulator) via cell-cell contact. the modulator activity was heat-labile and trypsin-sensitive. activity was also found in plasma membranes from kidney, brain, lung, and erythrocytes. the modulator was solubilized by 4% octylglucoside plus 4m guanidine hcl from liver membranes. the molecular weight of the modulator was 670kd dete ...19853985639
apple juice. an unappreciated cause of chronic diarrhea.chronic nonspecific diarrhea (cnsd) remains a common pediatric problem. previous reports have suggested disordered small intestinal motility, food intolerances, dietary fat restriction, and excessive fluid consumption as possible contributory factors. we have recently encountered a subset of children with cnsd in whom nonexcessive apple juice intake seemed to cause their diarrhea. in five subjects, ingestion of 240 ml of apple juice disclosed evidence of significant carbohydrate malabsorption by ...20143984976
ventricular shunt survival in children with neural tube defects.ventricular shunting has dramatically improved the care of children with hydrocephalus. yet shunt malfunctions are extremely common and cause significant morbidity. to document shunt problems in children with a neural tube defect and hydrocephalus, 67 children born since 1973 were studied via life-table analysis. 28% of the shunts failed within the first 6 months after insertion, 37% failed within the first year and 50% failed by 4 1/2 years after insertion. shunt survival was similar in childre ...20143984790
[determination of free serum phenol by gas-liquid chromatography in hemosorption].a modified procedure is developed for estimation of free phenol in blood serum by means of gas-liquid chromatography, appropriate in clinico-biochemical assays. the method enabled to evacuate the blood serum free phenol in healthy persons as well as in various pathologies of abdominal cavity before and after hemosorption. after hemosorption a distinct decrease of the free phenol content was observed in blood, correlating with improved clinico-biochemical patterns of the patients, with a decrease ...20143984277
differentiation of volume averaging and mass on magnetic resonance images of the mediastinum.volume averaging of high intensity mediastinal fat and low intensity flowing blood in mediastinal vessels can mimic a mediastinal mass on magnetic resonance (mr) images of the chest. however, when different repetition times (tr) are used to perform two imaging sequences, volume averaging can be distinguished from true disease, since the ratio of intensities of mediastinal fat, which is volume averaged, to nonvolume-averaged fat will remain relatively constant on a pair of images obtained using d ...20143983392
[case of olfactory groove meningioma with 6-year psychiatric anamnesis]. 20143983011
locus of control and effects of perceptual tasks on heart rate.the hypothesis was tested that the relationship found in prior research between heart rate changes produced in biofeedback settings and locus of control scores derives from the heart-brain relationship described by the laceys in 1967, as well as from "expectancies for control." 48 subjects were tested on two perceptual tasks known to elicit changes in heart rate. significant heart rate changes were observed in response to both tasks, but those changes did not correlate with locus of control as m ...20143982939
gentamicin disposition kinetics in humans with spinal cord injury.the disposition kinetics of gentamicin, an aminoglycoside antibiotic, were studied in seven tetraplegic and six paraplegic volunteers. the volume of distribution of gentamicin in l/kg of body weight varied in a statistically significant way from values of this parameter measured in normal subjects. the elimination of gentamicin in spinal man proceeded in a log-linear fashion accurately characterized by a one compartment open-model with a half-life of approximately 2 hours. the clinical significa ...19853982847
gentamicin disposition kinetics in humans with spinal cord injury.the disposition kinetics of gentamicin, an aminoglycoside antibiotic, were studied in seven tetraplegic and six paraplegic volunteers. the volume of distribution of gentamicin in l/kg of body weight varied in a statistically significant way from values of this parameter measured in normal subjects. the elimination of gentamicin in spinal man proceeded in a log-linear fashion accurately characterized by a one compartment open-model with a half-life of approximately 2 hours. the clinical significa ...19853982847
observations on facial nociception in a monkey after destruction of the rostral part of the trigeminal sensory nuclear complex.facial sensibility was assessed in a cynomolgus monkey in whom the trigeminal main sensory nucleus and the rostral part of the trigeminal nucleus oralis on one side had been destroyed. the animal responded equally to noxious mechanical stimuli applied to the two sides of the face; no deficiency in nociception could be detected. this finding suggests that the synaptic connections made by nociceptor afferents from the face in rostral parts of the trigeminal sensory nuclear complex are not essentia ...19853982839
the effects of acupuncture versus placebo in the treatment of headache.forty-eight patients were entered into a placebo (mock tns) versus acupuncture study to assess the effect of these therapies on headache. treatment was evaluated by the use of patient diaries; each patient completed a daily diary for 4 weeks prior to treatment during 6 weeks of therapy and for 24 weeks of follow-up. thirty-nine patients completed treatment and follow-up. at most acupuncture appears to be approximately 20% more effective than a placebo in alleviating headache but no statistically ...20143982837
posterior communicating artery giant aneurysm as a cause of seizures. 20143982666
surgical approaches to intraventricular meningiomas of the trigone.a case of a large intraventricular meningioma of the trigone is described. the tumor was removed by the transcallosal route. the neuropsychological sequelae of this approach are discussed. the value of other surgical approaches for meningiomas of the trigone is reviewed. the transcallosal approach originally described by kempe and blaylock is highly recommended.20143982626
skeletal muscle water and electrolytes in chronic renal failure. effects of long-term regular dialysis treatment.skeletal muscle water, cl, na and k were studied in 24 patients with predialysis chronic renal failure (crf) and in 16 patients under regular dialysis treatment (rdt) for 8-16 years; 35 healthy controls were also examined. total cl, na and water (clm, nam, tw) were high in both crf and rdt groups (p less than 0.001); high tw in crf was due to both extra (ecw) and intracellular (icw) fractions, which were calculated from cl space; in rdt only ecw was increased and icw was normal. muscle k was dim ...20133982577
[a case of metastatic carcinoma of the spermatic cord due to cholangioma].tumor in the spermatic cord is infrequent. further, among such cases, it is extremely rare to encounter a carcinoma other than a nonepithelial malignant tumor; almost all are metastatic tumors. we recently experienced an interesting case of spermatic cord tumor in a 78-year-old male. he entered our hospital with the chief complaint of a right inguinal tumor. he underwent 2 operations to excise the tumor. the extirpated tumor mass from the right spermatic cord was composed of adenocarcinoma with ...20143981798
metastatic nontraumatic clostridium septicum osteomyelitis.nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.19853980945
long-term results of tibiotalar arthrodesis.one hundred and one tibiotalar arthrodeses were performed using a single surgical technique that has not been previously reported. the average follow-up was ten years (range, two to twenty-five years). the rate of pseudarthrosis was 5 per cent, four to five times less than in other recent large reports. pseudarthrosis occurred only in patients with a sensory deficit. secure fusion was radiographically documented in 95 per cent and the functional clinical result was good to excellent in 90 per ce ...20103980499
identification and characterization of vibrio cholerae surface proteins by radioiodination.whole cells and isolated outer membrane from vibrio cholerae (classical, inaba) were radiolabeled with iodogen or iodo-beads as catalyst. radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine were found in radiolabeled outer membrane preparations obtained by a lithium chloride-lithium acetate pr ...19853980099
detection of circulating antigens in sera of rabbits infected with toxoplasma gondii.experiments were performed to investigate the usefulness of an enzyme-linked immunosorbent assay utilizing avidin-biotin interaction as a diagnostic tool for detection of toxoplasma antigen in blood. the lower limit of sensitivity of the assay by this method was ca. 4 ng/ml, and standard assays provided a linear plot of antigen concentration over a range up to 250 ng/ml. in rabbits inoculated subcutaneously with trophozoites of the rh strain, toxoplasma antigen became demonstrable in the circula ...19853980090
[study of the bacterial population of distilled water]. 19853979871
[detection of sulfolanes in sanitary-chemical analysis of textiles]. 20133979868
[detection of sulfolanes in sanitary-chemical analysis of textiles]. 20133979868
[food toxicology as a part of nutrition hygiene and a training course]. 20143979864
hepatic estrogen receptors and plasma estrogen-binding activity in the atlantic salmon.livers of male and female immature atlantic salmon (salmo salar) contain specific high-affinity [3h]estradiol binding sites in cytosol (kd 2-4 nm, concentration about 0.6 pmol/g liver). low levels of high-affinity binding are detectable in salt extracts of nuclei of untreated fish, but injections of estradiol result in transient depletion of the cytosol binder and in accumulation of high levels of binding sites in nuclear salt extracts (kd 5-6 nm; concentration about 6 pmol/g liver). both the cy ...20143979805
[anorectal motility in idiopathic constipation. study of 200 consecutive patients].the aim of our work was to study anorectal motility and tension of the rectal wall in 200 constipated adult patients. anorectal manometry was normal in 88 patients (group a), showed abnormal amplitude of the anorectal inhibitory reflex in 33 patients (group b) and hypertonia and ultraslow waves in 70 patients (group c). these anomalies were: a) reproducible in the 20 patients studied twice; b) observed in patients with enterocolitis, thus not specific of constipation; c) associated with an incre ...20143979721
clinical and laboratory features of an outbreak of vibrio cholerae o1 infections in the united states.a point source outbreak of vibrio cholerae o1 el tor inaba infections occurred aboard an oil rig south of port arthur, texas, in september 1981. sixteen crew members had v. cholerae o1 infections as determined by serology or stool specimens; 15 were symptomatic. the high percentage of symptomatic infections was attributed in part to the ingestion of a large number of v. cholerae o1 organisms by susceptible individuals. symptoms included diarrheal stools (100%), weakness (60%), abdominal cramps ( ...19853979022
inadvertent lymphoscintigraphy?several examples of what is believed to be lymph node uptake of the bone imaging agent tc-99m imidodiphosphate (tc-99m idp) are presented. this agent may prove to be useful for lymphoscintigraphy.20133978971
dermatoglyphic findings in patients with fragile x-chromosome.finger- and palmar prints of hemi- and heterozygote fragile x-patients with mental retardation (10 males and 5 females) were compared to dermatoglyphic findings in 20 mentally retarded patients (10 males and 10 females) without fragile x and to 200 healthy unrelated persons (100 males and 100 females). characteristic whorls and double-loops with high ridge-counts on finger-tips and a pronounced transversal course of palmar ridges were restricted to males with fragile x. female carriers of fragil ...20133978845
british medical association attacks cigarette smoking. 20143978493
enhancement of reactive oxygen-dependent mitochondrial membrane lipid peroxidation by the anticancer drug adriamycin.mitochondrial degeneration is a consistently prominent morphological alteration associated with adriamycin toxicity which may be the consequence of adriamycin-enhanced peroxidative damage to unsaturated mitochondrial membrane lipids. using isolated rat liver mitochondria as an in vitro model system to study the effects of the anticancer drug adriamycin on lipid peroxidation, we found that nadh-dependent mitochondrial peroxidation--measured by the 2-thiobarbituric acid method--was stimulated by a ...19853977958
reflex sympathetic dystrophy. 20143977580
the potential hazards of dinitrochlorobenzene. 19853977355
a new mutant strain of syrian hamster with myelin deficiency.a new disorder of central myelination was found in black mutant hamsters showing trembling. the condition was inherited in an autosomal recessive mode. histologically, a marked myelin deficiency was distributed throughout the central nervous system (cns), and it was closely related to clinical manifestations observed in the mutants ultrastructurally, abnormally thin myelin sheaths were observed in all axons with different diameters, and most axons below 1 micron in diameter were unmyelinated in ...19853976366
neuraminic acid is involved in the binding of influenza c virus to erythrocytes.neuraminidases of both viral and bacterial origin have been reported to be unable to destroy the cellular receptor for influenza c virus on chicken erythrocytes, in contrast to the receptors for influenza a and b virus. however, under appropriate conditions neuraminidases from both vibrio cholerae and clostridium perfringens were able (i) to make chicken red blood cells resistant against agglutination by influenza c virus and (ii) to reduce the hemagglutination-inhibiting activity of rat serum. ...19853976175
injury prevention in the community: a systems approach.injury prevention requires a coordinated political, medical, individual, and community effort. comprehensive strategies for planning, implementing, and evaluating injury prevention programs are set forth by the authors.20143975094
sylvian lipoma causing auditory hallucinations: case report.a sylvian fissure lipoma that caused auditory hallucinations, frequent olfactory auras, and occasional visual hallucinations is reported. the patient had been diagnosed as suffering from a functional psychosis for more than a decade, with symptoms severe enough to warrant frequent psychiatric attention, including institutional care. the computed tomographic findings are discussed. the literature is reviewed and therapeutic options are proposed.20143974815
tension pneumocephalus after evacuation of chronic subdural hematoma and subsequent treatment with continuous lumbar subarachnoid infusion and craniostomy drainage.we present a case of tension pneumocephalus after burr hole evacuation of bilateral chronic subdural hematomas. subsequent treatment was effected with combined twist drill closed system drainage and continuous intrathecal infusion of a physiological solution. the clinical entity, tension pneumocephalus, and the use of continuous subarachnoid infusion and drainage as a method of cerebral reexpansion are discussed.20143974807
blood transfusion and athletics. games people play. 20143974666
[neuroarthropathy as the initial sign of syringomyelia]. 20143974339
[late results of the surgical treatment of pancreatic pseudocysts following acute pancreatitis]. 20143974145
effects of neuronal activity on inositol phospholipid metabolism in the rat autonomic nervous system.the effect of nerve stimulation on inositol phospholipid hydrolysis in autonomic tissue was assessed by direct measurement of [3h]inositol phosphate production in ganglia that had been preincubated with [3h]inositol. within minutes, stimulation of the preganglionic nerve increased the [3h]inositol phosphate content of the superior cervical sympathetic ganglion indicating increased hydrolysis of inositol phospholipids. this effect was blocked in a low ca2+, high mg2+ medium. it was also greatly r ...19853973589
brain functioning and addiction: what neuropsychologic studies reveal. 20143973516
relationships between terminal transferase expression, stem cell colonization, and thymic maturation in the avian embryo: studies in thymic chimeras resulting from homospecific and heterospecific grafts.terminal deoxynucleotidyl transferase (tdt) can be detected in 11- to 12-day-old embryonic chick thymuses 5 to 6 days after the first influx of lymphoid stem cells into the thymic rudiment. to identify the main factors of tdt induction, grafting experiments were devised in such a way that the age of the grafted thymus and that of the host were different. uncolonized embryonic chick thymuses were grafted into chick hosts of different ages. under these conditions, lymphoid differentiation arose fr ...20133973385
model utilization in evaluation of a dial-a-dietitian system.the purpose of this study was to determine the effectiveness of selected factors of performance of a telephone delivery system, dial-a-dietitian, which was established to provide nutrition information to the public. a model, developed for this use, entailed a systems approach with five phases, which included enabling factors (inputs), the interaction process, effort factors (outputs), performance (outcome), and feedback (reporting). the data analysis indicated that the dial-a-dietitian program w ...20143973325
evaluation of immediate adverse reactions to foods in adult patients. ii. a detailed analysis of reaction patterns during oral food challenge.eighty-three oral food challenges were performed on 25 patients with a history of immediate adverse reaction to foods. seventy-one food challenges were performed in 24 patients, whereas 12 placebos were administered to nine patients. of the 71 food challenges observed, 12 were positive in 10 patients. all challenges with placebo were negative. doses of challenge foods provoking observable reactions ranged from 5 to 100 gm. the clinical signs and symptoms noted on food challenge reproduced those ...20133973310
evaluation of immediate adverse reactions to foods in adult patients. ii. a detailed analysis of reaction patterns during oral food challenge.eighty-three oral food challenges were performed on 25 patients with a history of immediate adverse reaction to foods. seventy-one food challenges were performed in 24 patients, whereas 12 placebos were administered to nine patients. of the 71 food challenges observed, 12 were positive in 10 patients. all challenges with placebo were negative. doses of challenge foods provoking observable reactions ranged from 5 to 100 gm. the clinical signs and symptoms noted on food challenge reproduced those ...20133973310
non-o1 vibrio cholerae in thailand: homology with cloned cholera toxin genes.we examined 281 non-o1 vibrio cholerae isolates from thailand for homology with genes coding for cholera toxin. five isolates from environmental sources were homologous with the cholera toxin gene probe and produced both the a and b subunits of cholera toxin.19853973005
developmental changes of the lipidic part of the neutral glycosphingolipids of the rat stomach.neutral glycolipids were purified from the glandular part of the stomach of rats of different ages from 20 days of gestation to 60 days after birth. the two major glycolipids were identified as glucosylceramide and isogloboside. free ceramide was also detected. the concentrations of these sphingolipids remained almost stable with development. monohexosylceramide contained 55 and 68% of 2-hydroxylated fatty acids at 20 and 22 days of gestation, respectively, and 82% in the adult. its three major ...19853972786
identification and occurrence of vibrio cholerae flagellar core proteins in isolated outer membrane.sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of outer membranes from a flagellated and an isogenic nonflagellated strain of vibrio cholerae (classical, inaba) suggested that two proteins were absent from the nonflagellated strain. immunoblot examination of such preparations demonstrated that two proteins, present only in outer membrane from the flagellated strain, were associated with flagella. analysis of purified flagellar cores from strains ca401 and n16961 (el tor, inaba) b ...19853972446
some problems associated with adenylate cyclase bioassays for parathyroid hormone.a renal adenylate cyclase system was assessed for its suitability in the performance of parathyroid hormone (pth) bioassays. membrane preparations from 1 week old chicks were found to be more sensitive to pth than material from humans, dogs or rats. because of the presence of non-specific inhibitors and stimulators of adenylate cyclase in human serum, each serum sample was assayed in the presence and in the absence of a specific pth inhibitor and the difference was used to calculate pth activity ...19853971661
lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks.effects of the bilateral electrolytic lesioning of the basal forebrain (bf), including the ventral globus pallidus, on passive or active avoidance tasks, were studied in male wistar rats. a severe deficit in acquisition of passive avoidance response was produced by the lesioning in the posterior level of bf. the retention of the passive avoidance response was markedly disrupted with post-training lesioning. time-dependent but only slight recovery from the memory impairments was observed in the p ...19853971182
spontaneous resolution of cervical warty atypia: the relevance of clinical and nuclear dna features: a prospective study.a selected group of 51 patients with histological features of cervical warty atypia was observed for 1 year. initial features were compared with a cohort of 50 patients with cin. twenty-four patients showed resolution of the warty atypia, in 19 it remained unchanged, and in eight patients cin or microinvasive disease was present on repeat biopsy. the dna patterns obtained by tissue imprint of the initial biopsies and subsequent integrated densitometry, using a vickers m85 scanning densitometer, ...20143970896
infusion rates for i.v. anaesthesia. 20143970803
amelioration of cyclosporin-induced nephrotoxicity in rats by induction of hepatic drug metabolism.the aim of this study was to determine the effect of altered hepatic drug metabolism on the nephrotoxic and immunosuppressive properties of cyclosporin a (csa) in the rat. from a consideration of the structures of those csa metabolites identified so far, it seemed probable that the metabolism of csa would occur at the hepatic cytochrome p-450 (cyt p-450) enzyme system. csa (50 mg/kg/24 hr) administered orally for 14 days resulted in significant increases in both serum urea concentration and urin ...19853970725
transient hyperphosphatasaemia. 20103970581
crystal formation after reconstituting cefazolin sodium with 0.9% sodium chloride injection. 20143970029
severe vibrio cholerae sepsis and meningitis in a young infant. 19853969638
[cholescintigraphy in the diagnosis of biliary reflux after esophageal and gastric operations]. 20143969284
invasion of privacy. 20143969230
management of chronic pain.pain is an unpleasant, subjective experience which escapes objective measurements. it is the normal response to the disruption of healthy tissues, but its persistence beyond the period of healing should alert the practitioner to other unrecognized causes. on the other hand, learned pain is a condition resulting mainly from socio-emotional factors in the absence of progressive, invasive and, most of the time, demonstrable pathology. the key to success in the management of a painful condition is a ...20143969229
further studies on the role of neuraminidase and the mechanism of low ph dependence in influenza virus-induced membrane fusion.the role of neuraminidase and the mechanism of low ph dependence in influenza virus-induced membrane fusion have been studied further using fowl plague virus (fpv, h7n1). two specific anti-fpv neuraminidase antisera obtained from chickens immunized with recombinant virus strains inhibited viral neuraminidase activity without influencing its haemagglutinating activity. these sera totally inhibited the fpv-induced fusion of erythrocytes and partially reduced haemolysis. but both fusion and haemoly ...19853968541
seroepidemiological studies of el tor cholera in bangladesh: association of serum antibody levels with protection.in rural bangladesh, family contacts of patients with cholera were studied prospectively to examine whether protection against colonization and disease due to vibrio cholerae o1 was associated with circulating antibodies to v. cholerae. family contacts (1,071) of 370 patients with cholera were visited daily for 10 days, cultured for v. cholerae, and queried about diarrhea. sera collected on days 1 and 21 were assayed for vibriocidal antibodies, igg and iga antibodies to cholera toxin, and igg an ...19853968450
nifedipine-induced psychosis. 20143968373
occurrence of 2-keto-deoxyoctonic acid 5-phosphate in lipopolysaccharides of vibrio cholerae ogawa and inaba.a phosphorylated 2-keto-3-deoxyoctonic acid (kdo) was released from the lipopolysaccharides of vibrio cholerae ogawa and inaba after strong acid hydrolysis. the phosphorylated kdo was identified by gas-liquid chromatography and mass spectrometry after reduction and permethylation as kdo-5-phosphate and an isomer of it being phosphorylated at position 7 or 8. after treatment with alkaline phosphatase, kdo was detected by gas-liquid chromatography and mass spectrometry. it was indistinguishable fr ...19853968044
intestinal absorption of bacterial antigens in normal adult mice. ii. a comparative study of techniques.marked differences were seen in the oral absorption of boivin antigen (ba) and flagellin (fla) using antigens labelled by 3h-dinitrophenylation. thus, after feeding 3h-dnp-fla, a large proportion of the label was rapidly absorbed, concentrated and degraded by the liver so that no antigenic material could be recovered from the circulation. in contrast, the absorption of 3h-dnp-ba was low and slow; the absorbed material appeared stable and not readily taken up by the liver, so that 8-20% of the no ...19853967930
intestinal absorption of bacterial antigens in normal adult mice. i. preparation and characterization of the antigens.the preparation of flagellin (fla) from salmonella adelaide and boivin antigen (ba) from vibrio cholerae is described. these two antigens differed chemically from each other and showed marked differences in their degradability by mouse intestinal juice in vitro and in their fate in the intact mouse when given intravenously. unlike ba, fla was rapidly degraded in intestinal juice and readily sequestered and degraded by the liver. the suitability of these antigens in oral absorption studies is dis ...19853967929
iron-vibriobactin transport system is not required for virulence of vibrio cholerae.the possible requirement of a functional siderophore (vibriobactin)-mediated iron transport system in the pathogenicity of vibrio cholerae was determined. two mutants of v. cholerae defective in the iron-vibriobactin transport system were examined for their ability to multiply and elicit diarrhea in infant mice. one mutant, 40130, was unable to synthesize vibriobactin. the second mutant, 1510, was able to synthesize, but not transport, the siderophore. both mutants retained the ability to multip ...19853967921
current treatment modes for tuberculosis. public policy and implementation. 20123967520
current treatment modes for tuberculosis. public policy and implementation. 20123967520
elimination of malignant clonogenic cells from human bone marrow using multiple monoclonal antibodies and complement.a clonogenic assay has been developed that utilizes burkitt's lymphoma tumor cell lines to detect elimination of up to 5 logs of tumor cell contamination within human bone marrow. different burkitt's lymphoma lines bear one or more of a group of markers, including common acute lymphoblastic leukemia antigen gp26 (glycoprotein with a molecular weight of 26,000), b1, surface membrane immunoglobulin, hla, beta 2-microglobulin, and ia. burkitt's tumor cells of the namalwa line have been mixed with a ...19853967224
selective killing of contaminating human fibroblasts in epithelial cultures derived from colorectal tumours using an anti thy-1 antibody-ricin conjugate. 19853966968
pyostomatitis vegetans. a reactive mucosal marker for inflammatory disease of the gut.pyostomatitis vegetans is a rarely occurring disorder of the oral mucosa, which has a dramatic clinical picture and a striking histopathologic pattern. moreover, the condition is a highly specific oral mucosal marker for inflammatory bowel disease, particularly ulcerative colitis. our findings in two cases and a review of the literature emphasize the relationship of pyostomatitis vegetans to inflammatory bowel disease and the vegetating dermatoses.20143966824
flammable surgical drapes--a patient and personnel hazard.after a surgical drape fire, the new york state society of anesthesiologists, operating room safety committee, investigated the factors contributing to it. subsequently, eight detailed cases were collected. it found that no voluntary standards or government regulations exist to oversee the flammability of surgical drapes; no agency or bureau collects reports of operating room fires; most professionals are not aware of the hazard because no labelling requirements regarding flammability exist and ...20133966652
torsade de pointes: a maximal vulnerability arrhythmia. 20143966319
direct synthesis of low-density lipoprotein apoprotein b in the miniature pig.the metabolism of apoprotein b (apo b) was investigated in five miniature pigs following the injection of radiolabeled, very low-density lipoproteins (vldl). the fractional catabolic rate (fcr) for vldl apoprotein b was 0.71 +/- 0.10 h-1 (mean +/- se), the rate of flux was 0.77 +/- 0.05 mg h-1 kg-1, and the pool size of apoprotein b averaged 1.26 +/- 0.20 mg kg-1. examination of precursor-product relationships between vldl and low-density lipoprotein (ldl) apoprotein b illustrated that a signifi ...19853965861
effects of aging and illness on the pituitary testicular axis in men: qualitative as well as quantitative changes in luteinizing hormone.decreased testicular function occurs as a concomitant of aging in men and is accentuated by the presence of systemic illness. previous studies identified an intrinsic leydig cell defect, as reflected by high lh to testosterone ratios and impaired hcg responsiveness in older men. this study questioned whether a qualitative change in lh secretion, as reflected by secretion of lh with an altered ratio of biological to immunological lh (b/i) activity, might occur during aging. to examine this possib ...19853965490
psychological and attitudinal profiles in donors for artificial insemination.few objective data are available concerning the psychological profiles and attitudes of sperm donors in artificial insemination programs. we studied 30 consecutive new volunteers and 45 established sperm donors using the cattell 16pf personality profile and an attitudinal survey concerning motives for donation, attitudes toward uses of sperm, desire for knowledge of outcomes, and attitudes toward disclosures of identifying and nonidentifying information between the parties to artificial insemina ...20133965318
serum carotene levels in female long-distance runners.hypercarotenemia has been associated with anorexia nervosa and hypothalamic amenorrhea. with the emergence of a spectrum of menstrual dysfunction, including anovulation in women runners, the hypothesis of hypothalamic amenorrhea in these patients has been formulated. other authors have proposed exercise-associated amenorrhea to be distinct from hypothalamic amenorrhea based on endocrine profiles. using carotene as an index test of hypothalamic amenorrhea, we have studied female long-distance run ...20133965316
vancomycin-induced neutropenia.although rare, neutropenia associated with long-term vancomycin therapy may occur. a 67-year-old woman with cellulitis and sepsis caused by staphylococcus aureus was treated initially with cefazolin. despite in-vitro susceptibility of the organism to this drug, the patient remained febrile, and therapy was changed to vancomycin. on day 17 of therapy with this medication neutropenia was noted; it progressed over the next 3 days, and therapy with the drug was stopped. a rise in the neutrophil coun ...19853965060
vancomycin-induced neutropenia.although rare, neutropenia associated with long-term vancomycin therapy may occur. a 67-year-old woman with cellulitis and sepsis caused by staphylococcus aureus was treated initially with cefazolin. despite in-vitro susceptibility of the organism to this drug, the patient remained febrile, and therapy was changed to vancomycin. on day 17 of therapy with this medication neutropenia was noted; it progressed over the next 3 days, and therapy with the drug was stopped. a rise in the neutrophil coun ...19853965060
nerve growth factor: a chemotactic factor for polymorphonuclear leukocytes in vivo.previous studies have shown that mouse submandibular gland nerve growth factor (ngf) stimulates chemotactic migration of human polymorphonuclear leukocytes in vitro. the results of the present study demonstrate that subdermal injection of ngf in mice also stimulates rapid and marked chemotactic recruitment of leukocytes. this property of ngf is manifest in the nanomolar range of concentrations, it requires the known serine class protease activity of the growth factor, and it does not require par ...19853964821
the prediction of anatomical morphology of primary aldosteronism using serum 18-hydroxycorticosterone levels.serum 18-hydroxycorticosterone, aldosterone, and potassium were measured under basal conditions in 34 patients with documented primary aldosteronism, 10 patients with essential hypertension, and 9 normal subjects. the results revealed that 22 of 23 patients with aldosterone-producing adenomas had 18-hydroxycorticosterone levels greater than 100 ng/dl, and all 9 patients with idiopathic adrenal hyperplasia had plasma levels less than 100 ng/dl. two patients with unusual macromicronodular hyperpla ...20133964794
the prediction of anatomical morphology of primary aldosteronism using serum 18-hydroxycorticosterone levels.serum 18-hydroxycorticosterone, aldosterone, and potassium were measured under basal conditions in 34 patients with documented primary aldosteronism, 10 patients with essential hypertension, and 9 normal subjects. the results revealed that 22 of 23 patients with aldosterone-producing adenomas had 18-hydroxycorticosterone levels greater than 100 ng/dl, and all 9 patients with idiopathic adrenal hyperplasia had plasma levels less than 100 ng/dl. two patients with unusual macromicronodular hyperpla ...20133964794
one definition of asthma. 20143964738
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