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the response of campylobacter jejuni and campylobacter coli in the sydney rock oyster (crassostrea commercialis), during depuration and storage.sydney rock oysters, when allowed to feed in waters containing approximately 10(4) cfu of campylobacter cells per ml, concentrated between 10(2) and 10(3) cfu of the organism per g of oyster tissue, within 1 h. when these contaminated oysters were subjected to depuration, they were effectively cleaned in 48 h. the survival of campylobacter jejuni and campylobacter coli was also investigated. oysters contaminated by feeding and injection were processed as half shells and bottled oysters and were ...19883275320
heterozygosity at the km locus associated with humoral immunity to campylobacter jejuni.serum samples from 43 caucasian subjects convalescing from acute campylobacter jejuni infection were typed for nine gm and two km determinants. the sera were also used to measure iga, igg, and igm classes of antibody to acid-labile surface proteins of c. jejuni by an enzyme-linked immunosorbent assay. a highly significant association (p = 0.004) was found between km1/km3 heterozygotes and the level of iga antibodies. these results suggest the existence of complementary immune response genes whic ...19863274046
[campylobacter jejuni and campylobacter coli: epidemiologic markers of isolated strains in santiago, chile]. 19883267894
prevalence of campylobacter in infant, juvenile and adult laboratory primates.a cross-sectional study of campylobacter spp. infection was conducted on 125 infant (macaca nemestrina and macaca fascicularis) in an infant primate nursery housing infants from birth to 18 months of age, and 145 m. nemestrina aged from 4 months to 15 years at another facility (primate field station) housing animals from birth to aged adults. the objective was to determine the prevalence of campylobacter spp. in various age groups and to investigate the correlation with diarrhea. in the infant p ...19883265462
[bacteriological, pharmacokinetic and clinical studies on a rokitamycin dry syrup in the pediatric field].bacteriological, pharmacokinetic and clinical studies were done on the effect of rokitamycin (rkm, tms-19-q) in the field of pediatrics. the results are summarized below. 1. antibacterial activities antibacterial activities of rkm against staphylococcus aureus (including 50 methicillin-sensitive and 50 methicillin-resistant strains), 18 strains of haemophilus influenzae and 50 strains of campylobacter jejuni were studied comparatively with activities of josamycin (jm), midecamycin (mdm), erythro ...19883265445
paediatric campylobacter diarrhoea from household exposure to live chickens in lima, peru.although campylobacter jejuni is a frequent enteropathogen in cases of paediatric diarrhoea in developing countries, its route of transmission is not well understood. an age-matched, case-control study of children with c. jejuni diarrhoea was therefore carried out in lima, peru, from january 1983 to april 1986 to identify the risk factors and vehicles of transmissions. as cases, 104 children less than 3 years of age were selected and compared with controls of the same age with non-gastrointestin ...19883262442
the occurrence of thermophilic campylobacter in mink and an experimental oral infection of pregnant mink by campylobacter jejuni. 19883256243
comparative in vitro activities of new 14-, 15-, and 16-membered macrolides.the in vitro activities of several 14-, 15- and 16-membered macrolides were compared with that of erythromycin. in general, 14-membered macrolides such as erythromycin, clarithromycin, and flurithromycin were more active against streptococci and bordetella pertussis than was the 15-membered macrolide azithromycin, which was more active than 16-membered macrolides such as miocamycin and rokitamycin. clarithromycin was the most active compound against streptococcus pyogenes, pneumococci, listeria ...19883252753
acute diarrhoeal disease in nigeria: detection of enteropathogens in a rural sub-saharan population.a retrospective study was carried out to obtain epidemiological data concerning the relative risk of shigella, salmonella, campylobacter jejuni and vibrio cholerae infection in several rural population groups. faecal samples (rectal swab specimens) were cultured from 914 selected children with acute diarrhoeal disease and from a matched control group of 984 non-diarrhoeal children. shigella were more frequently isolated than the other enteropathogens from both the diarrhoeal (12.7%) and control ...19883252601
antibodies against campylobacter jejuni/coli in patients suffering from campylobacteriosis or inflammatory bowel disease.sera from 74 patients from the oslo area and 21 from northern norway with compylobacteriosis were analysed for antibodies against an antigen mixture comprising seven campylobacter jejuni/coli strains including a pen 0:6,7 isolate representing the most common serotype in northern norway. seventy-seven per cent of the campylobacter patients from the oslo area and 86% from northern norway had elevated antibody levels. thirty-seven out of 57 (65%) seropositive patients from oslo and 12 out of 18 (67 ...19883247063
effect of mucin on campylobacter jejuni association and invasion on hep-2 cells.we report the development of an in vitro assay using hep-2 cell monolayers in which cell association of five c. jejuni strains and e. coli k12 c600 were tested under different experimental conditions. both total cell associated colony forming units (cfu) and the cfu protected from gentamicin killing (i.e. internalized bacteria) were determined. pretreatment of hep-2 cells with mucin (100 micrograms/ml) enhanced internalization of four fresh isolates of c. jejuni by 3.2 to 20.7-fold, depending on ...19883244310
an outbreak of campylobacter jejuni gastroenteritis at a resort--ontario. 19883242891
comparative in vitro potencies of nine new macrolides.the in vitro activities of the 14-membered macrolides (14m) clarithromycin (cl), roxithromycin (ro), dirithromycin (di) and flurithromycin (fl), a 15-membered macrolide (15m) azithromycin (az), and the 16-membered macrolides (16m) josamycin (jm), spiramycin (sp), miocamycin (mi) and rokitamycin (rk) were compared with erythromycin (er). cl was two- to four-fold more potent than er against all organisms except haemophilus and campylobacter jejuni against which it was two-fold less active. ro and ...19883240705
[survival of campylobacter jejuni in brain and heart extracts of different ph]. 19883238294
incidence and level of campylobacter jejuni in broiler processing.the distribution and level of campylobacter jejuni throughout broiler processing was evaluated. six trials were conducted at three broiler processing plants to determine the occurrence of c. jejuni on commercial broilers as affected by specific processing functions. counts were determined at specific sampling stations throughout the plants, and patterns of counts among sampling stations and plants were observed. between 1,100 and 5,500 c. jejuni/1,000 cm2 skin surface (represents half the skin s ...19883237574
role of the 92.5-kilodalton outer membrane protein of campylobacter jejuni in serological reactions.outer membrane proteins from 12 strains of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against formalin-treated cell suspensions of three of the strains. the 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, ...19883230126
antibacterial antibody pattern in seronegative spondyloarthropathies (snsa).the occurrence of some anti-bacterial antibodies was studied in sera from 31 healthy donors (hd) and 101 patients with different rheumatic diseases. the cases investigated included 7 psoriatic arthritis (pa), 35 rheumatoid arthritis (ra), 17 undifferentiated seronegative spondyloarthritis (u-snsa), 13 behçet's syndrome, 18 enteric arthropathies (ea), 7 ankylosing spondylitis (as) and 4 reiter's syndrome. a complement fixation test was carried out to detect the presence and to evaluate the titer ...19883229028
survival of coliforms and bacterial pathogens within protozoa during chlorination.the susceptibility of coliform bacteria and bacterial pathogens to free chlorine residuals was determined before and after incubation with amoebae and ciliate protozoa. viability of bacteria was quantified to determine their resistance to free chlorine residuals when ingested by laboratory strains of acanthamoeba castellanii and tetrahymena pyriformis. cocultures of bacteria and protozoa were incubated to facilitate ingestion of the bacteria and then were chlorinated, neutralized, and sonicated ...19883223766
[which are the most frequently occurring enteropathogenic bacteria in clinical practice?].diarrhea is an increasingly frequent clinical finding in swiss medical practice. one reason is the volume of tourism in countries with different climatic and social circumstances. to obtain an overview of the enteropathogenic bacteria spectrum as a cause of diarrhea, we analyzed 11,163 stool specimens from a representative group of patients throughout switzerland. enteropathogenic bacteria were found in 15.2% of all specimens; 50% were campylobacter jejuni/coli, 35% salmonella spp., 8% aeromonas ...19883217775
effect of incubation temperature, ageing, and bisulfite content of unsupplemented brucella agar on aerotolerance of campylobacter jejuni.a mutant strain of campylobacter jejuni atcc 29428 was isolated that grows on unsupplemented brucella agar at o2 levels as high as 21% at 37 degrees c. while measuring the degree of aerotolerance of this mutant on unsupplemented brucella medium and comparing it with that of the wild type, we found considerable variation among our estimates. as measured by colony counts on unsupplemented brucella agar incubated at various oxygen levels, the degree of aerotolerance was affected by incubation tempe ...19883214811
surface charge and hydrophobicity of campylobacter jejuni strains in relation to adhesion to epithelial ht-29 cells.hydrophobicity and surface charge of clinical isolates of campylobacter jejuni strains were investigated by aqueous two-phase partitioning (one-step and counter-current distribution), ion exchange chromatography and hydrophobic interaction chromatography. there was a good correlation between the different physico-chemical methods reflecting the same bacterial property. all strains were negatively charged and exposed a hydrophobic surface, but to a varying extent. bacteria with a high negative su ...19883214584
[effect of water activity on the growth of campylobacter jejuni in brain-heart infusion]. 19883212356
antimicrobial susceptibility of campylobacter jejuni, campylobacter coli, and campylobacter laridis from cats, dogs, pigs, and seagulls. 19883210481
antimicrobial sensitivity and plasmid-mediated tetracycline resistance in campylobacter jejuni isolated in bangladesh.the antimicrobial sensitivity of campylobacter jejuni isolated in bangladesh from patients with diarrhoea, asymptomatic carriers and domestic animals was performed. all isolates were sensitive to erythromycin, gentamicin, furazolidone and kanamycin. seven percent isolates were resistant to tetracycline, 8% to nalidixic acid, 37% to ampicillin and 100% to sulfamethoxazole-trimethoprim and cephalothin. tetracycline resistance was observed to be plasmid mediated. no plasmid band(s) coding for ampic ...19883208549
yield of stool cultures, ova and parasite tests, and clostridium difficile determinations in nosocomial diarrheas.stool cultures for bacterial pathogens, ova and parasites, and clostridium difficile are usually ordered for patients with nosocomial diarrhea. in the interest of cost containment we undertook an 18-month retrospective study to assess the cost/benefit of performing each of these three tests. during the study period nosocomial diarrhea developed in 118 patients. of 452 bacterial stool cultures ordered, only one was positive for campylobacter jejuni and none for ova and parasites. however, of 126 ...19883207206
[prevalence of campylobacter jejuni/coli in personnel and animals of the zoo park of santiago. analysis of the pathogenic factors]. 19883205994
the occurrence of campylobacter jejuni in raw cows' milk.faeces and raw milk from individual cows were examined for the presence of campylobacter jejuni. after drawing milk, the lactoperoxidase system was inactivated by raising the ph to 7.5. the organism was isolated from 22% of 904 faecal samples and from 4.5% of 904 milk samples. from laboratory experiments it could be concluded that inactivation of the lactoperoxidase system resulted in a better isolation of c. jejuni from raw cows' milk.19883204072
selective association and transport of campylobacter jejuni through m cells of rabbit peyer's patches.m cells in the peyer's patches may facilitate transport of pathogens such as campylobacter jejuni from the intestine. we evaluated this hypothesis by using electron microscopy to examine peyer's patches in ligated adult rabbit ileal loops inoculated with 5-ml suspensions of 10(9) cfu/ml of campylobacter jejuni. peyer's patches taken at intervals from 15 min to 2 h after inoculation of loops in anaesthetized rabbits provided evidence that campylobacter jejuni selectively adhered to m cells as opp ...19883196964
in vitro activities of pd 117,596 and reference antibiotics against 448 clinical bacterial strains.the in vitro activity of pd 117,596, a new fluoroquinolone antibiotic, was tested against 448 bacterial isolates (15 genera) by agar dilution (inoculum, 10(4) cfu per spot). the activity of pd 117,596 was compared with that of 15 antibiotics against 327 gram-negative strains and with that of 8 other antibiotics against 121 gram-positive strains. pd 117,596 demonstrated the best activity against klebsiella spp., enterobacter spp., acinetobacter spp., serratia marcescens, and branhamella catarrhal ...19883196008
incidence of campylobacter jejuni in processed egg products.egg samples were obtained from two commercial egg-processing facilities to determine if campylobacter jejuni could be isolated from the raw product or from further-processed egg products intended for human consumption. no c. jejuni were detected in any of the egg or egg-product samples or from water samples collected from the overflow of the egg washer. campylobacter jejuni was recovered from all control samples, which had been inoculated at the plants. these findings suggest that properly proce ...19883194336
is enrichment culture necessary for isolating campylobacter jejuni from faeces? 19883192739
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
methemoglobinemia and hemolytic anemia associated with campylobacter jejuni enteritis.a 7-week-old infant with methemoglobinemia, hemolytic anemia, and inadequate weight gain was found to have a campylobacter jejuni gastrointestinal tract infection. known etiologies of methemoglobinemia such as oxidative drug exposure, deficiency of nadh-methemoglobin reductase, and hemoglobin m disorder were excluded. the patient had a twin brother (probably identical) who had neither methemoglobinemia nor stool cultures positive for c. jejuni. the twin essentially served as an experimental cont ...19883189715
an outbreak of food-borne campylobacter jejuni infection and the possible role of cross-contamination.a point-source outbreak or campylobacter infection affected 24 of 51 delegates attending a business lunch. failure to detect any specific vehicle for the infection led to observations of kitchen practice. experiments demonstrated that cross-contamination as a result of handling raw and cooked food consecutively was a possible cause of the outbreak.19883183409
isolation of campylobacter jejuni from the bile of a cholecystic patient.described is the isolation of campylobacter jejuni from the bile of a cholecystic patient admitted for abdominal pain to an intensive care unit. a cholecystectomy was performed, and the patient responded to erythromycin therapy. details of this uncommon isolation are given.19883183005
the comparative in-vitro activity of ofloxacin.the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...19883182468
the pathogenicity of environmental campylobacters--a human volunteer experiment.three human volunteer experiments were performed in which river water expected to contain campylobacter organisms was ingested. despite the ingestion of over 44,000 organisms in one experiment, the subject did not suffer any symptoms, nor were campylobacter organisms excreted, nor was an antibody response to the ingested strains detected. the campylobacter organisms ingested resembled campylobacter jejuni on colonial and microscopic morphology but were hippurate negative, and were distinct from ...19883181313
campylobacters in man and the environment in hull and east yorkshire.campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ...19883181312
[laboratory and clinical studies of rokitamycin dry syrup in the field of pediatrics].laboratory and clinical studies on rokitamycin (rkm) dry syrup, a new macrolide antibiotic, were carried out in the field of pediatrics. the results are summarized as follows. 1. plasma concentrations and urinary recovery rates after oral administration on fasting of rkm dry syrup at doses of 10 mg/kg and 20 mg/kg to 2 and 1 cases, respectively, were determined. peak plasma levels were obtained in 30 minutes after administration of both dosages with half-lives of 1.5 to 2.2 hours. a clear-cut do ...19883172461
isolation of a gastric campylobacter-like organism from the stomach of four rhesus monkeys, and identification as campylobacter pylori.campylobacter-like organisms, isolated from the gastric antrum of rhesus monkeys, were compared with campylobacter jejuni and c. pylori. they were similar to c. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with rabbit antisera to c. jejuni and c. pylori and with c. pylori-specific monoclonal antibody. because this natural infection of the rhesus monkey is associated with chronic gastritis ...19883172170
a specific dna probe for the identification of campylobacter jejuni.a 6.1 kb dna probe for the human enteric pathogen campylobacter jejuni has been isolated from a genomic library constructed in the plasmid vector pbr322 in escherichia coli. the dna sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of c. jejuni. restriction endonuclease fragment mapping of c. jejuni dna inserts from three of the recombinant plasmids showed an overl ...19883171548
peroxide sensitivity and catalase activity in campylobacter jejuni after injury and during recovery.cells of campylobacter jejuni damaged by freezing or mild heat showed increased sensitivity to hydrogen peroxide and also required the addition of catalase for growth on nutrient agar. these phenomena, which could have important practical consequences, were not associated, however, with changes in total catalase activity and may be due to alterations in outer membrane permeability.19883170383
[serologic diagnosis of campylobacteriosis. determination of antibodies to the outer membrane protein of campylobacter jejuni by the elisa method]. 19883167988
isolation of campylobacter jejuni from quails: an initial report. 19883167556
[susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates].susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ...19883163383
[digestive localizations of sexually transmitted diseases in male homosexuals].the common practice of sexual relations with many different and anonymous partners and the great variety of responsible micro-organisms account for the high incidence, growing complexity and uneasy prevention of sexually transmitted diseases of the digestive tract in male homosexuals. syphilis, gonorrhoea, papillomas, chancroid, donovanosis, herpes virus or chlamydia infections are known to be transmitted by anal coitus; amebiasis, giardiasis and shigellosis by oro-anal contact (faecal contamina ...19853160035
in-vitro activity of pefloxacin compared to enoxacin, norfloxacin, gentamicin and new beta-lactams.the in-vitro activity of pefloxacin was compared with that of norfloxacin, enoxacin, nalidixic acid, gentamicin, cefotaxime, ceftazidime and, where appropriate, other beta-lactams against a total of 363 recent clinical isolates. an agar dilution procedure was used to determine mics and two inocula (10(4) and 10(6) cfu) were used throughout. pefloxacin inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-positive proteus spp., enterobacter spp., shigella ...19853156112
[isolation of campylobacter fetus subsp. jejuni from feces and transport media during a diarrhea epidemic]. 19853155666
[campylobacter jejuni infections in slaughterhouse workers].complement fixing (c.f.) antibodies to campylobacter jejuni were detected in 83 slaughterhouse workers and 83 blood donors. workers were aged 18-65 years (mean, 41.7 +/- 12.3) and had worked in the slaughterhouse for 2-40 years (mean, 17.5 +/- 5.1). c.f. antibodies were detected according to mosimann's method and by including five antigens: campylobacter jejuni, yersinia enterocolitica types 03 and 09, yersinia pseudotuberculosis and brucella. positive titers were found in 12.1% of workers and i ...19883154982
a mouse model of chronic campylobacter jejuni infection. post-infection nephritis. 19883148404
[use of slide agglutination technic in serogrouping of campylobacter jejuni/coli isolates from the outbreaks and sporadic cases in japan]. 19883143789
risk factors for persistent diarrhoea.with a systematically sampled population of children aged under 5 attending this centre for diarrhoeal disease research during 1983-5 a retrospective analysis of persistent diarrhoea (defined as greater than 14 days' duration) was performed to identify the possible risk factors for this syndrome. of the 4155 children included in the analysis, 410 (10%) gave a history of persistent diarrhoea. a comparison with children with acute diarrhoea matched for age showed that 11 factors were correlated wi ...19883142603
further serological investigations in humans and domestic animals on the cape verde islands (q-fever, brucellosis, listeriosis, shigellosis, campylobacteriosis, yersiniosis, toxoplasmosis and chlamydia of plt-group).439 sera of domestic animals and various number (150-500) of human sera of the cape verde islands were examined for antibodies against coxiella burnetti, brucella abortus, b.melitensis, listeria monocytogenes, shigella dysenteriae, s.flexneri, s. boydii, s.sonnei, campylobacter jejuni, c.fetus ssp. intestinalis, yersinia enterocolitica, toxoplasma gondii and chlamydia of the plt-group. it could be shown, that on the cape verde islands there is to be counted with numerous zoonoses due to various ...19883139491
[survey for the contamination of campylobacter jejuni and campylobacter coli among raw meat and the environment of poultry processing plants]. 19883137280
human serum antibody response in campylobacter jejuni enteritis as measured by enzyme-linked immunosorbent assay.an elisa for detection of igg, iga, and igm antibody using an acid-glycine extract from campylobacter jejuni as antigen was developed. to determine the value of this assay for the diagnosis of acute campylobacter jejuni infections, the igg, iga, and igm immune response against campylobacter jejuni was investigated at various timepoints after infection in patients with culture-proven infection. a total of 112 sera from 46 patients and 78 sera from a control group were tested. all but one of the 4 ...19883137040
reassessment of selective agars and filtration techniques for isolation of campylobacter species from faeces.four different studies were conducted in order to re-evaluate conventional methods and assess the efficacy of new selective agars and a filtration method for the isolation of campylobacters. skirrow's medium, preston agar, modified ccd agar and fennell's medium, incubated microaerobically at 37 degrees c for 48 h, gave similar campylobacter isolation rates from 225 faecal samples, but the latter two media were more selective. evaluation of modified ccd agar demonstrated that campylobacters could ...19883134202
[quantitative and qualitative studies of campylobacter in a sewage treatment plant].the occurrence and distribution of campylobacter spp. in the wastewater of a sewage treatment plant was examined from july 1985 to july 1986. the number of campylobacter was determined in a double set-up by the mpn-method. preston broth and -agar containing fbp supplement were used as nutrient media. the samples were incubated at 37 degrees c and 42 degrees c, respectively. subsequently 1345 isolated campylobacter strains were biotyped. the number of fecal and total coliforms, ph-value and tempe ...19883131984
[quantitative and qualitative studies of campylobacter in the sewage of a large city].in a one-year-study wastewater from the sewage system of a big town was examined for the occurrence and distribution of campylobacter spp. the sewage consisted mainly of domestic and industrial wastewater, but also to a small degree of agricultural wastewater and abattoir effluent. preston broth and agar were used as culture media. the number of campylobacter was determined parallel at 37 degrees c and 42 degrees c by the mpn-method. three colonies of each agar plate were isolated and biotyped. ...19883131983
[modification of intestinal colonization and translocation of campylobacter jejuni by erythromycin and an extract of lactobacillus acidophilus in axenic mice].erythromycin (15 mg) or lyophylized heat-killed lactobacillus acidophilus (80 mg) were administered per os daily in germ-free mice infected at day o with 10(7)-10(8) campylobacter jejuni per os. bacterial colonization of intestine and bacterial translocation of c. jejuni toward mesenteric lymph nodes, blood and liver were then studied for 5 days. the results were compared with those from an infected untreated control group. compared to the control group, the numbers of free and mucosa-associated ...19863122638
antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease.egg white lysozyme was demonstrated to have antibacterial activity against organisms of concern in food safety, including listeria monocytogenes and certain strains of clostridium botulinum. we also found that the food spoilage thermophile clostridium thermosaccharolyticum was highly susceptible to lysozyme and confirmed that the spoilage organisms bacillus stearothermophilus and clostridium tyrobutyricum were also extremely sensitive. several gram-positive and gram-negative pathogens isolated f ...19873118808
[campylobacter jejuni in poultry processed in slaughterhouses].the frequency of occurrence of campylobacter jejuni germs in dressed poultry was studied for a year. the samples--smears from the body cavities of chickens--were collected during the technological dressing of the chickens; 101 strains of campylobacter jejuni (i. e. 28.69%) were isolated from the 352 samples analyzed. the occurrence of the germs exhibited a considerable seasonal variance with peak rates in spring and summer. the use of a suitable culture medium, the technique of cultivation and t ...19873118554
[campylobacter jejuni and its incidence in meat packaged under warm conditions].the problem of survival of campylobacter jejuni was studied in artificially contaminated butchered meat packed in warm condition. apart from the presence of campylobacter jejuni, the total number of microorganisms and the shelf life of the meat were studied. with plain packing, campylobacter jejuni survived six to seven days and the shelf life of the meat was about a week. in vacuum-packed meat campylobacter jejuni was isolated for ten to eleven days and sensory changes occurred after ten to fou ...19873118552
[comparative in vitro activity of 10 new 4-quinolones against enteropathogenic bacteria].the susceptibility has been determined of 169 shigella spp., 93 salmonella spp., 68 yersinia enterocolitica, 34 vibrio cholerae 0:1, and 34 campylobacter jejuni, isolated in central africa and in belgium, to ten fluoroquinolones: ciprofloxacin, ofloxacin, norfloxacin, pefloxacin, enoxacin, amifloxacin, ci-934, a-56619, a-56620 and ro 23-6240. all quinolones were active against the strains tested, showing an mic90 between 0.004 mg/l and 2 mg/l for strains susceptible to nalidixic acid and an mic9 ...19873116489
potential pathogenic mechanisms of animal-passed virulence-enhanced strains of campylobacter jejuni/coli. 19873108554
[a food-borne infection caused by campylobacter jejuni serotype lauwers 19].report on a outbreak due to contaminated food involving 556 schoolchildren who got their lunch from a school kitchen. the outbreak occurred abruptly without any secondary infectious. examinations for salmonellae, shigellae, enteritis-coli, yersiniae and rotaviruses were negative. however, campylobacter jejuni were isolated from 105 specimens of faeces in rostock. sixty of these strains and another 2 that had been isolated at the same time but had no relation to the outbreak in rostock, were furt ...19863107256
[prevalence of campylobacter jejuni complement fixing antibody in healthy people]. 19863097197
[biotyping of campylobacter jejuni isolated from diarrhea patients]. 19863095457
in vitro antibacterial activity of enoxacin (ci-919).antibacterial activity of enoxacin was evaluated against more than 3,700 clinical isolates using the agar-dilution method and an inoculum of 10(4)-10(5) cells per site. for comparison other antibiotics appropriate for each species were also included. for most enterobacteria and for staphylococcus aureus, staphylococcus epidermidis and pseudomonas aeruginosa, the mic90 of enoxacin was below 2 mg/l. serratia marcescens was more resistant; the mic90 being 4 mg/ml. enoxacin also showed high activity ...19863093961
macrolide, lincosamide and streptogramin resistance in campylobacter jejuni/coli.strains of erythromycin-resistant campylobacter jejuni/coli isolated in the united kingdom, canada and europe, were also resistant to tylosin, spiramycin and clindamycin and, like sensitive strains, were insensitive to virginiamycin and pristinamycin component b. these were designated as macrolide-lincosamide-streptogramin generalized resistant organisms and macrolide-lincosamide generalized resistant on the basis of patterns of resistance to pristinamycin components a and b. two erythromycin-se ...19863084440
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter spp.to determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...19863079730
a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...19883076719
campylobacter.campylobacter is now known to be a major cause of gastrointestinal illness throughout the world. there are seven species known to be associated with enteritis, and it is likely that more will be described. campylobacter jejuni is responsible for more than 95 per cent of the cases of diarrhea due to campylobacter. contaminated food products are the major source of infection. the clinical illness usually involves diarrhea, abdominal pain, and other constitutional symptoms. an accurate diagnosis of ...19883074120
campylobacter and salmonella contamination of unpasteurized cows' milk on sale to the public.campylobacters and salmonellas were isolated respectively from 6 and 0.2% of samples of unpasteurized cow's milk on sale to the public. there was a significant association between the presence of escherichia coli and that of campylobacter jejuni. the mean e. coli count was also higher in campylobacter-positive samples. enumeration of e. coli would seem to have value as an indicator of faecal contamination and thus potential hazard in raw milk.19883072328
the growth of potential food poisoning organisms on chicken and pork muscle surfaces.muscle surfaces of pork were inoculated with a mixture of yersinia enterocolitica and staphylococcus aureus, and chicken muscle with campylobacter jejuni or a mixture of salmonella typhimurium and staph. aureus. the surface growth at 20 degrees c was followed microscopically. organisms grew as discrete colonies bound together by a glycocalyx which differed between bacterial species. on prolonged incubation colonies spread peripherally and tended to coalesce, while still retaining their colony st ...19883072327
cloning and expression of the campylobacter jejuni glya gene in escherichia coli.genetic studies of campylobacter jejuni are greatly hampered by the lack of genetic markers and an established classical gene transfer mechanism between strains of this species. to facilitate future genetic studies and to provide a recombinant dna approach for analyzing genes of c. jejuni, we constructed an extensive genomic library of a pathogenic c. jejuni strain tgh9011 (serotype 0:3) using pbr322. we report the isolation of a number of recombinant plasmids containing the complete structural ...19883072255
comparison of broth enrichment and direct plating for the isolation of campylobacter jejuni from dogs.two techniques for the isolation of campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. a total of 272 rectal swab cultures were performed on 156 laboratory dogs. campylobacter blood agar plates and campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. after incubation at 4 degrees c for 12 to 16 h, material from the campylobacter thioglycolate medium was inoculated onto campylobacter blood agar plates. a total of ...19883069857
oral rehydration in infantile diarrhoea in the developed world.acute diarrhoea is an important health problem in developed countries, particularly in young children. the attack rates for viral diarrhoea are similar in developed and developing countries. rotavirus is the most common pathogen, followed by adenovirus. bacterial diarrhoea is less common in developed than developing countries. the 2 most common bacterial pathogens are campylobacter jejuni and salmonella. the most serious consequence of diarrhoea is dehydration, and the treatment for this is the ...19883069444
the genus campylobacter: a decade of progress.in 1977, microbiologists and clinicians were awakened to the importance of the genus campylobacter when it was learned that one species, campylobacter jejuni, was a major cause of human enteritis. in the following decade substantial advances were made in diagnosis, isolation technology, identification, classification, serotyping, and epidemiology. the genus has undergone rapid expansion as advantage was taken of the deoxyribonucleic acid-deoxyribonucleic acid hybridization technique in defining ...19883069194
[isolation of a campylobacter jejuni-like bacteria from gastric mucosa]. 19883063580
pathogenic bacteria in milk--a review.pathogenic bacteria are transmissible to humans through milk and milk products. recently, attention is focused on milk, cheese, and ice cream contaminated with pathogenic bacteria, viz., listeria monocytogenes, yersinia enterocolitica, campylobacter jejuni, and enteropathogenic escherichia coli. this review presents information on characteristics of these pathogens and illnesses caused by them.19883060492
colonization of gastrointestinal tracts of chicks by campylobacter jejuni.bacterial enumeration and histologic examination of organs and tissues of 8-day-old chicks 7 days after peroral inoculation with campylobacter jejuni revealed that the organism colonized primarily the lower gastrointestinal tract. the principal sites of localization were the ceca, large intestine, and cloaca, where densely packed cells of c. jejuni were observed in mucus within crypts. examination of c. jejuni-colonized crypts by transmission electron microscopy revealed that the campylobacters ...19883060015
[chronic diarrhea caused by campylobacter jejuni in a patient with aids].we report a case of c. jejuni chronic diarrhea in a homosexual man with aids. the antibiotic susceptibility of the bacteria was about normal for the species. investigating specific immunity, we observed the presence of anti-c. jejuni igg and the absence of anti-c. jejuni iga. these findings concord with the hypothesis that specific iga are necessary for c. jejuni clearance from the digestive tract.19883059270
characterization of campylobacter jejuni and campylobacter coli isolated from waste water.the raw sewage samples of the investigated municipal sewage plant (sewage flow of about 30,000 m3/d) contained about 10(3) campylobacters/100 ml. the sewage plant reduced 80.8% of organic wastes (cod), 85.0% of colony counts (20 degrees c), 92.5% of total coliforms and 88.0% of campylobacters. the sewage plant effluent contained an average of 1.3 x 10(2) campylobacters/100 ml. about 10(10) campylobacters are discharged with the effluent of the high-output sewage plant into the receiving water ev ...19883057769
motility of campylobacter jejuni in a viscous environment: comparison with conventional rod-shaped bacteria.the motility of four strains of campylobacter jejuni in solutions of varying viscosity was measured and compared to that of a number of conventional rod-shaped bacteria (crsb). all the bacteria tested showed an initial increase in velocity in the low viscosity solutions--between 1 and 3 centipoise (1 p = 0.1 pa s). however, only the campylobacters were actively motile in highly viscous solutions with velocities ranging from 60 to 100 micron s-1. all strains of c. jejuni tested showed three separ ...19883053972
immune response to acute diarrhea seen as circulating antibody-secreting cells.we studied the immune response to acute diarrhea by examining antibody-secreting cells among peripheral blood lymphocytes, which are believed to be derived from the intestinal mucosa and to be on their way back there. in 23 of 24 patients, a dramatic increase in the total number of cells actively secreting immunoglobulins was detected one week after onset of diarrhea, and most of the cells were secreting iga. cells secreting antibody specific to the pathogen (campylobacter jejuni or salmonella s ...19883053918
the clinical use of macrolides.macrolides are active against streptococcus pneumoniae, legionella spp. and mycoplasma pneumoniae, the main causes of community-acquired pneumonia they may therefore be used for the empirical treatment of community-acquired pneumonia, although emergent resistance in str. pneumoniae limits their use in some parts of the world. in patients with bronchitis the use of macrolides reduces the severity and duration of symptoms. macrolides have also been used successfully in the treatment of otitis medi ...19883053568
electrophoretic protein typing of campylobacter jejuni subspecies "doylei" (nitrate-negative campylobacter-like organisms) from human faeces and gastric mucosa.twenty-seven strains comprising 23 clinical isolates of nitrate negative campylobacters (nnc) from australia, south africa, the united kingdom and the federal republic of germany, a representative of the cnw (catalase negative/weak) group and reference strains of three other campylobacter species, were characterized by one-dimensional sds-polyacrylamide gel electrophoresis of cellular proteins. the protein patterns were highly reproducible, and were used as the basis for a numerical analysis whi ...19883053235
occurrence of 'thermophilic' campylobacters in sewage and their removal by treatment processes.removal of thermophilic campylobacters from sewage at three different stages of treatment at a trickling filter sewage works has been assessed. samples of incoming sewage, primary sedimentation effluent and final effluent were taken daily from 06.00 h to 20.00 h for 5 consecutive days and the numbers of campylobacters determined by using a most probable number method. each sample was cultured using 2 h pre-enrichment followed by enrichment in preston broth for 48 h and detection by plating. over ...19883053218
[microbiological, pharmacokinetic and clinical studies of rokitamycin dry syrup in the pediatric field].rokitamycin (rkm), a newly developed macrolide antibiotic with a 16-membered ring, dissolves well under acidic conditions. it has been improved over other macrolides to minimize individual variations in its absorbability. we measured, using the ga-test, variations in gastric acidities of 43 children with ages between 1 to 14 years, and investigated the relationship between gastric acidities and pharmacokinetic values. also activities (expressed in mics) of antimicrobial agents were studied again ...19883050186
adherence of campylobacter jejuni and campylobacter coli to porcine intestinal brush border membranes.the adhesion of campylobacter jejuni and c. coli to isolated porcine intestinal brush border membranes was studied by phase-contrast and electron microscopy. approximately 45% of the cell population adhered to the brush borders, possibly in a specific manner. pretreatment of the brush borders with trypsin or pronase, and competitive inhibition with l-rhamnose caused a slight reduction of the adhesion. different forms of pretreatment of the bacterial cells reduced their ability to adhere, but als ...19883046640
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
genetic studies of kanamycin resistance in campylobacter jejuni.campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...19863021049
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...19863020082
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...19863017528
pathogens that cause travelers' diarrhea in latin america and africa.with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ...19863014633
incidence of antibiotic resistance and characterization of plasmids in campylobacter jejuni strains isolated from clinical sources in alberta, canada.antibiotic susceptibilities of 382 strains of campylobacter jejuni isolated in alberta, canada, in 1980 and 1981 were determined. although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. tetracycline resistance was noted in 6.8% of strains in 1980 and in 8.6% in 1981. moreover, resistance to high-level tetracycline (32-128 micrograms/ml) was always mediated by a plasmid of 45-50 kilobases. three transmissible ...19863008970
enteropathogens associated with acute diarrhea in hospitalized infants.thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ...19863007718
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