Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| construction of the temperature-sensitive vectors pluch80 and pluch88 for delivery of tn917::noti/smai and use of these vectors to derive a circular map of listeria monocytogenes scott a, a serotype 4b isolate. | a physical map of listeria monocytogenes scott a was generated by the pulsed-field technique of contour-clamped-homogeneous-electric-field (chef) electrophoresis. the circular genome of this serotype 4b strain contains 12 asci fragments (38 to 790 kb), 5 noti fragments (55 to 1,400 kb), 3 srfi fragments (110, 1,110, and 2,000 kb), and 2 sfii fragments (1,320 and 1,920 kb). summation of individually sized fragments derived by digestion of scott a genomic dna with each of these four enzymes provid ... | 1997 | 9292998 |
| modifications of membrane phospholipid composition in nisin-resistant listeria monocytogenes scott a. | a nisin-resistant (nisr) variant of listeria monocytogenes scott a was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (bhi) broth. the nisr strain was about 12 times more resistant to nisin than was the wild-type (wt) strain. accordingly, higher nisin concentrations were required to dissipate both components of the proton motive force in the nisr strain than in the wt strain. comparison of the membrane fatty acyl composition of the sensitive strain wi ... | 1997 | 9292996 |
| evaluation of seven experimental phages for inclusion in the international phage set for the epidemiological typing of listeria monocytogenes. | the purpose of our study was to evaluate the inclusion of seven experimental phages into the international phage set for subtyping listeria monocytogenes. the seven additional phages included the broad-host-range virulent myoviridae phage a511 (m. j. loessner, appl. environ. microbiol. 57:1912-1918, 1991), three temperate phages from the danish subsystem for typing serotype 1/2 strains (12682, 6223, and 5775) (p. gerner-smidt, v.t. rosdahl, and w. frederiksen, apmis 101:160-167, 1993), and three ... | 1997 | 9292987 |
| alternative antigen processing pathways in anti-infective immunity. | proteinaceous and nonproteinaceous antigens from exogenous microorganisms can be processed by the host for mhc class i restricted presentation to t cells. macrophages, b cells, mast cells and dendritic cells are antigen-presenting cells that process such exogenous antigens through multiple pathways before mhc-restricted epitope presentation. new conceptual frameworks are emerging regarding the processing and presentation to t cells of peptide or nonpeptide epitopes from bacteria in the context o ... | 1997 | 9287179 |
| lymphocyte apoptosis during early phase of listeria infection in mice. | during the acute phase of growth of listeria monocytogenes in spleen and lymph nodes, the infective foci consist of macrophages and neutrophils accompanied by extensive death of lymphocytes. many of the lymphocytes die by apoptosis. the lesions are found by 48 hours after infection and can regress with time. depending on the dose, the infected foci can be restricted to the thymus-dependent areas or can occupy the entire lymphoid tissue. the listeria in the lesions are primarily found inside macr ... | 1997 | 9284827 |
| listeriolysin and irpa are major protein targets of the human humoral response against listeria monocytogenes. | we have examined the human humoral immune response directed against proteins of listeria monocytogenes in both healthy individuals and listeriosis patients. two major targets for an antibody response were found in individuals that did not suffer from listeriosis: listeriolysin (hly) and the recently described internalin-related protein (irpa). in contrast, the humoral response in listeriosis patients appears to be more heterogeneous and included hly, irpa, inlb, and acta as major targets. | 1997 | 9284184 |
| inlb: an invasion protein of listeria monocytogenes with a novel type of surface association. | listeria monocytogenes is an intracellular bacterial pathogen that expresses several surface proteins critical for the infectious process. such proteins include inla (internalin) and inlb, involved in bacterial entry into the host cell, and acta, required for bacterially induced actin-based motility. although the molecular mechanisms of attachment of inla and acta have been characterized, essentially nothing is known about how inlb is anchored to the bacterial surface. using a genetic approach, ... | 1997 | 9282740 |
| foodborne listeriosis. | various epidemiological investigations of outbreaks and sporadic cases have clearly demonstrated that the consumption of contaminated food is responsible for a high proportion of listeriosis cases and listeria monocytogenes has been increasingly recognized as an important foodborne pathogen over the last 15 years. the emergence of listeriosis is the result of complex interactions of different factors: medical progress which increases the lifespan and allows immunodeficient people to survive, exp ... | 1997 | 9282388 |
| economic costs and trade impacts of microbial foodborne illness. | this article presents the economic costs of foodborne diseases for selected countries, the approaches used to calculate these costs, and a discussion on the interaction between microbial food safety issues and international trade in food. the human illness costs due to foodborne pathogens are estimated most completely in the united states of america, where, each year, 7 foodborne pathogens (campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7. listeria monocytogenes, salmonell ... | 1997 | 9282387 |
| the effect of high hydrostatic pressure on listeria monocytogenes in phosphate-buffered saline and model food systems. | three strains of listeria monocytogenes (nctc 1194, a poultry isolate and the scott a strain) were exposed to a range of pressures (300, 350, 375, 400 and 450 mpa) in 10 mmol 1(-1) phosphate-buffered saline (pbs) at ph 7.0 for up to 30 min at ambient temperature. generally, increasing the magnitude and duration of compression resulted in increasing levels of inactivation, although the inactivation kinetics varied depending on the strain and pressure applied. the three strains also exhibited a wi ... | 1997 | 9281822 |
| absence of il-1 signaling and reduced inflammatory response in il-1 type i receptor-deficient mice. | il-1alpha and il-1beta are potent inflammatory cytokines that contribute to a number of normal physiologic processes and to the development of a number of inflammatory diseases. two il-1r, the type i and type ii receptors, have been identified. this work describes the derivation and characterization of mice deficient in expression of the type i il-1r (il-1ri). il-1ri-deficient mice were viable and fertile, but failed to respond to il-1 in a variety of assays, including il-1-induced il-6 and e-se ... | 1997 | 9278338 |
| [escape mechanism of listeria monocytogenes in relation to host immune response]. | 1997 | 9277105 | |
| safe poultry meat production in the next century. | the revolutionary industrialisation of the poultry industry in the last 30 years has made the food poultry meat available for large groups of consumers. due to its nutritional, sensory and economical characteristics, poultry meat is by far the most popular animal food product world-wide. epidemiological reports, however, incriminate poultry meat as a source for outbreaks of human food poisoning. the organisms involved are salmonella spp., campylobacter spp. and, to a lesser extent, listeria mono ... | 1997 | 9276991 |
| comparative sensitivity of 13 species of pathogenic bacteria to seven chemical germicides. | the relative resistance of diverse human bacterial pathogens to commonly used germicidal agents has not been established. | 1997 | 9276546 |
| spatial interactions between subsurface bacterial colonies in a model system: a territory model describing the inhibition of listeria monocytogenes by a nisin-producing lactic acid bacterium. | the effect of spatial separation on interactions between subsurface bacterial colonies was tested using a model system: the inhibition of listeria monocytogenes by nisin-producing and nisin-non-producing lactococcus lactis subsp. lactis. separation distance was controlled by altering the number of inoculum organisms within the agar. mean separation distance was calculated by determining the mean volume available to each cell at the start of the experiment. inhibition was assessed by comparing th ... | 1997 | 9274011 |
| gastroenteritis caused by listeria monocytogenes in a private day-care facility. | 1997 | 9271054 | |
| production of nisin-like bacteriocins by lactococcus lactis strains isolated from vegetables. | four bacteriocin producing lactic acid bacteria isolated from vegetables were identified as lactococcus lactis strains on the basis of physiological and biochemical characteristics, carbohydrate fermentation patterns and analysis of total soluble protein pattern by sds page. the bacteriocins had a wide spectrum of activity as antagonism was detected not only towards a variety of lactic acid bacteria, but also to staphylococcus aureus and listeria monocytogenes. these bacteriocins were resistant ... | 1997 | 9265741 |
| listeria monocytogenes potently induces up-regulation of endothelial adhesion molecules and neutrophil adhesion to cultured human endothelial cells. | infection of endothelial cells by listeria monocytogenes is an essential step in the pathogenesis of listeriosis. listeriolysin (hly) is one of its major virulence factors. in the early phase of the disease polymorphonuclear leukocytes (pmn) substantially contribute to the nonspecific anti-listerial resistance. we characterized the effects of l. monocytogenes on the expression of endothelial adhesion molecules and on subsequent pmn adhesion to cultured huvec. p-selectin, e-selectin, intracellula ... | 1997 | 9257863 |
| listeria monocytogenes infection of p388d1 macrophages results in a biphasic nf-kappab (rela/p50) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by ikappabalpha and ikappabbeta degradation. | as previously reported, listeria monocytogenes infection of p388d1 macrophages results in a rapid induction of nf-kappab dna-binding activity. here we show that this induction of nf-kappab activity occurs in a biphasic mode: first, a transient, ikappabalpha degradation-dependent phase of activity, also induced by the nonvirulent species listeria innocua, which is mediated by binding of the bacteria to the macrophage, or by adding listeria-derived lipoteichoic acid to the macrophage; the second p ... | 1997 | 9256493 |
| fatal listeria meningitis, endocarditis and pericarditis in a patient with haemochromatosis. | a 65-year-old man with primary haemochromatosis was admitted because of fever and confusion. he was found to have bacteraemia and meningitis due to listeria monocytogenes. treatment with ampicillin plus tobramycin was instituted, and despite an initial improvement, the patient experienced an unfavourable course and died. at postmortem examination, tricuspid valve endocarditis and purulent pericarditis with tamponade were detected. listeria monocytogenes grew in the culture of the pericardial flu ... | 1997 | 9255896 |
| effects of several factors on the heat-shock-induced thermotolerance of listeria monocytogenes. | the influence of the temperature at which listeria monocytogenes had been grown (4 or 37 degrees c) on the response to heat shocks of different durations at different temperatures was investigated. for cells grown at 4 degrees c, the effect of storage, prior to and after heat shock, on the induced thermotolerance was also studied. death kinetics of heat-shocked cells is also discussed. for l. monocytogenes grown at 37 degrees c, the greatest response to heat shock was a fourfold increase in ther ... | 1997 | 9251209 |
| host-mediated modification of sau3ai restriction in listeria monocytogenes: prevalence in epidemic-associated strains. | most major food-related outbreaks of listeriosis have been traced to a cluster of genetically related strains of serovar 4b (epidemic clone). in spite of numerous searches, distinct bacteriologic or virulence-related features unique to these strains have eluded identification, although a restriction fragment length polymorphism (rflp) characteristic of the epidemic clone has previously been described (w. zheng and s. kathariou, appl. environ. microbiol. 61:4310-4314, 1995). we found that dnas fr ... | 1997 | 9251194 |
| evaluation of luciferase reporter bacteriophage a511::luxab for detection of listeria monocytogenes in contaminated foods. | a511::luxab is a recombinant derivative of a broad-host-range bacteriophage specific for the genus listeria, transducing bacterial bioluminescence into infected cells. in this study, we have evaluated its use for rapid and easy testing of contaminated foods and environmental samples for the presence of viable listeria cells, in comparison to the standard plating procedure. with a short preenrichment step of 20 h, the system was capable of detecting very low initial contamination rates in several ... | 1997 | 9251182 |
| a gerbil model for rhombencephalitis due to listeria monocytogenes. | rhombencephalitis due to listeria monocytogenes is a frequent complication of human listeriosis, inducing a high mortality and severe neurological sequelae despite antibiotic therapy. however, there is no animal model which consistently reproduces clinical rhombencephalitis. here, we present a model of listeria rhombencephalitis in gerbils. animals were inoculated in the middle ears with a low infective dose of l. monocytogenes, thus creating prolonged otitis media with persistent bacteremia. ge ... | 1997 | 9250779 |
| an outbreak of listeriosis in a breeding colony of chinchillas. | 1997 | 9249176 | |
| the effect of high hydrostatic pressure on the activity of intracellular enzymes of listeria monocytogenes. | the effect of high hydrostatic pressure (100-550 mpa, 15 min, ambient temperature) on the activity of 13 metabolic enzymes produced by all three strains of listeria monocytogenes (nctc 11994, a poultry isolate and scott a) was examined using gel electrophoresis. the enzymes assayed exhibited a wide variation in barotolerance. the pressure resistance of each particular enzyme was not dependent on the strain from which it was derived. this would seem to indicate that these enzymes were not a deter ... | 1997 | 9248081 |
| antigen-specific t cell receptor antagonism by antigen-presenting cells treated with the hemolysin of listeria monocytogenes: a novel type of immune escape. | we have examined the influence of listeriolysin o (llo), the hemolysin secreted by the pathogenic bacterium listeria monocytogenes, on major histocompatibility complex class ii-dependent t cell activation. stimulation of t cells by native antigens but not by peptides is inhibited upon pretreatment of antigen-presenting cells (apc) with llo. experiments presented here reveal that this inhibition is not due to a lack in processing of antigen by apc but is the result of an irreversible inactivation ... | 1997 | 9247580 |
| antimicrobial activity of food-related penicillium sp. against pathogenic bacteria in laboratory media and a cheese model system. | food-related penicillium species (n = 34) and geotrichum candidum (n = 11) grown on czapek dox and brie agar were tested for their ability to suppress growth of pathogenic bacteria. ten out of 13 p. camemberti showed antagonistic activity while the other species did not interact significantly with the bacterial growth. the order of inhibition was: gram-negative bacteria and bacillus cereus > listeria monocytogenes, lactococcus sp. > micrococcus sp. whereas lactobacillus sp., staphylococcus aureu ... | 1997 | 9246777 |
| the effect of temperature, ph and medium in a surface adhesion immunofluorescent technique for detection of listeria monocytogenes. | a rapid surface adhesion-based immunofluorescence technique was used to detect listeria monocytogenes from inoculated culture systems. the effect of culture type (pure, mixed and meat), ph (7.00, 6.40, 4.76 and 3.13), acids (citric and hcl) and temperature (25 degrees, 30 degrees and 37 degrees c) on the adhesion of listeria to the polycarbonate membrane used in this technique was determined. it was found that ph had a significant effect (p < 0.05) with higher numbers of listeria adhering at low ... | 1997 | 9246776 |
| microbiological composition of raw milk from selected farms in the camembert region of normandy. | raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, yersinia enterocolitica and campylobacters. total bacterial counts and somatic cell counts were measured. lactococci, lactobacilli, dextran-producing leuconostocs, brevibacterium linens, yeasts and moulds, staphylococcus aureus and other micrococcaceae, pseudomonas, coliforms, escherichia coli, enterococci, clostridium perfringens and spores of anaerobic lactate-fermenting bacteria were also counted. pseudomonas (2000 c ... | 1997 | 9246770 |
| physiological and biochemical studies on psychrotolerance in listeria monocytogenes. | studies were undertaken to explain the ability of listeria monocytogenes to grow at low temperatures in a chemostat. it was found that when grown in continuous culture at a dilution rate of 0.02 h-1 l. monocytogenes had a lower proportion of anteiso-17:0, and a higher proportion of anteiso-15:0, and smaller chain fatty acids when grown at 10 degrees c compared to 30 degrees c. a previously unreported glycolipid was only seen after growth at low temperature. growth temperature had no effect on th ... | 1997 | 9246768 |
| conjugative transfer of tet(s) between strains of enterococcus faecalis is associated with the exchange of large fragments of chromosomal dna. | the tetracycline resistance determinant tet(s) was first detected in antibiotic multiresistant listeria monocytogenes bm4210 and subsequently in strains of enterococcus faecalis. transfer of tet(s) from clinical isolate e. faecalis bm4242 to e. faecalis strains jh2-2 and og1rf was found to require the presence in the donor strain of the 55 kb conjugative plasmid pip825. comparison of restriction endonuclease generated maps of the donor, the two recipients, and of four transconjugants indicated t ... | 1997 | 9245804 |
| cd8alphaalpha t cells in lesions of listeria monocytogenes-infected beta2m-deficient mice. | the beta2-microglobulin (beta2m)-deficient mutant mice lack alphabeta tcr cd8alphabeta t cells. we found markedly impaired granuloma formation in listeria monocytogenes-infected beta2m-/- mice. abundant cd8alphaalpha t cells were identified in loosely structured infiltrative liver lesions. microfluorescence analysis disclosed that these cd8alphaalpha t cells expressed mostly the gammadelta tcr. cd8alphaalpha t cells were also found in the spleen of listeria-infected beta2m-/- mice. these data pr ... | 1997 | 9245621 |
| [role of starlings in the diffusion of listeria monocytogenes and listeria spp]. | 1996 | 9244465 | |
| [presence of listeria spp. in vegetables]. | 1996 | 9244463 | |
| a case of congenital listeria septicemia associated with high levels of inflammatory cytokines. | a case of congenital listeria septicemia is reported. a 2256 g male infant suffering from respiratory and circulatory failure with shock-like symptoms and high levels of inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1 beta, -6, and -8), was admitted to the morioka red cross hospital. listeria monocytogenes was cultured from cord blood, contents from the external ear canal, rectum and stomach. the infant was treated with surfactant replacement as well as conventional therapy. t ... | 1997 | 9241908 |
| predictive microbiology of dairy products: influence of biological factors affecting growth of listeria monocytogenes. | the growth potential of listeria monocytogenes was evaluated at low temperature in sterilized milk and raw dairy products. sterilized and raw milk were inoculated with different strains of l. monocytogenes in 2 physiological states and at various contamination levels. raw cheese was naturally contaminated with listeria spp. the results suggest that some biological factors influence the growth capacity of l monocytogenes in dairy products. significant strain effect was observed at low temperature ... | 1997 | 9241852 |
| visual immunoprecipitate assay (vip) for listeria monocytogenes and related listeria species detection in selected foods: collaborative study. | six foods representing a variety of food products were analyzed by the visual immunoprecipitate assay (vip) and either the bacteriological analytical manual (bam) or the u.s. department of agriculture culture methods for detection of listeria monocytogenes and related listeria spp. samples of each food type at each inoculation level were simultaneously analyzed by both methods. a total of 23 laboratories representing federal agencies and private industry in the united states and canada participa ... | 1997 | 9241843 |
| assurance polyclonal enzyme immunoassay for detection of listeria monocytogenes and related listeria species in selected foods: collaborative study. | six foods representing a variety of food products were analyzed by the assurance listeria polyclonal enzyme immunoassay (eia) and by either the bacteriological analytical manual or the u.s. department of agriculture culture method for detecting listeria monocytogenes and related listeria species. samples of each food type, at each inoculation level, were analyzed simultaneously by both methods. a total of 19 laboratories representing federal government agencies and private industry in the united ... | 1997 | 9241842 |
| cell wall-deficient forms (l-forms) of listeria monocytogenes in experimentally infected rats. | experimental infections were induced with different bacterial forms of listeria monocytogenes: parental (s-forms), protoplastic (l-forms) and combined inoculum of both forms by i.p. injection of rats. the parental bacterial forms (s-forms) were isolated up to 7 days after challenge from the peritoneal cavity and the liver, while the l-forms were isolated up to 60 days from the peritoneal cavity. continuous adhesion of l-forms on the peritoneal macrophage surface was found by scanning-electron mi ... | 1997 | 9241800 |
| typing of austrian listeria monocytogenes isolates by automated laser fluorescence analysis of randomly amplified polymorphic dna. | we used automated laser fluorescence analysis of randomly amplified polymorphic dna (rapd-alfa) to study the epidemiology of listeriosis in western austria. there were no discrepancies between rapd-alfa patterns and serotypes found in 18 food isolates and 18 clinical isolates. the results of our study suggest that the food isolates typed were not at the origin of the human cases in western austria. using rapd-alfa, it was possible to link 9 out of 16 "sporadic" listeria infections (mother-child ... | 1997 | 9241798 |
| emerging issues in microbiological food safety. | many microorganisms previously unrecognized as food-borne or harmful are emerging as human pathogens transmitted by food. this is a result of recent acquisition of key virulence factors, detection by newly developed isolation procedures, or astute detective-like laboratory skills of microbiologists. six microbial pathogens, including shiga toxin-producing escherichia coli, listeria monocytogenes, arcobacter butzleri, helicobacter pylori, cryptosporidium parvum, and cyclospora, have become recogn ... | 1997 | 9240928 |
| prevalence of salmonella, listeria monocytogenes, campylobacter spp., yersinia enterocolitica and cryptosporidium spp. in bulk milk, cows' faeces and effluents of dairy farms in trinidad. | the prevalence of listeria, salmonella, campylobacter, yersinia and cryptosporidium species in bulk milk, faeces of dairy cows and effluents of milking parlours from dairy farms in trinidad was investigated. of the 177 bulk milk samples studied, 3 (1.7%), 3 (1.7%) and 2 (1.1%) were positive for l. monocytogenes, salmonella spp. and y. enterocolitica, respectively, but were all negative for campylobacter and cryptosporidium species. from 333 faecal samples tested, l. monocytogenes, campylobacter ... | 1996 | 9239938 |
| augmentation of host defence against bacterial and fungal infections of mice pretreated with the non-pathogenic escherichia coli strain nissle 1917. | escherichia coli strain nissle 1917 (dsm 6601, mutaflor) was investigated for its ability to enhance the immune response against bacterial or fungal infections in vivo. mice were infected intravenously with either 6 x 10(3) colony forming units (cfu) of listeria monocytogenes bacteria or 5 x 10(5) candida albicans cells. one day prior to infection, mice were treated orally with four different concentrations of e. coli strain nissle 1917 (10(6), 10(7), 10(8), and 10(9) viable cells). three days a ... | 1997 | 9239462 |
| predictive model of the effect of co2, ph, temperature and nacl on the growth of listeria monocytogenes. | the growth responses of l. monocytogenes as affected by co2 concentration (0-100% v/v, balance nitrogen), nacl concentration (0.5-8.0% w/v), ph (4.5-7.0) and temperature (4-20 degrees c) were studied in laboratory medium. growth curves were fitted using the model of baranyi and roberts, and specific growth rates derived from the curve fit were modelled. predictions for specific growth rate, doubling time and time to a 1000-fold increase could be made for any combination of conditions within the ... | 1997 | 9237120 |
| rapid purification, partial characterization, and antimicrobial spectrum of the bacteriocin, pediocin acm, from pediococcus acidilactici m. | the bacteriocin from pediococcus acidilactici m, designated as pediocin acm, was rapidly purified to homogeneity by the ph mediated cell adsorption-desorption method and semi-preparative reversed-phase hplc. the purification yield was 40.4% and the specific activity was increased by 2450-fold. it gave a single band and a single peak on sds-page and hplc analysis, respectively. when subjected to electrospray lc-ms analysis, the protein was found to be highly pure and the molecular weight was dete ... | 1997 | 9237116 |
| the listeria monocytogenes-secreted p60 protein is an n-end rule substrate in the cytosol of infected cells. implications for major histocompatibility complex class i antigen processing of bacterial proteins. | cytosolic antigen degradation is an initial step in the generation of major histocompatibility complex (mhc) class i-associated cytolytic t lymphocyte epitopes. intracellular listeria monocytogenes secretes p60, a murein hydrolase, into the host cell cytosol, where it is degraded by proteasomes. roughly 3% of degraded p60 gives rise to p60 217-225, a nonamer peptide that is bound by h-2kd mhc class i molecules. herein, we introduce targeted deletions throughout the p60 gene to identify potential ... | 1997 | 9235920 |
| multiple control elements mediate activation of the murine and human interleukin 12 p40 promoters: evidence of functional synergy between c/ebp and rel proteins. | interleukin 12 (il-12) is a heterodimeric cytokine whose activity is critical for t-helper 1 responses. the gene for the il-12 p40 subunit is expressed in macrophages following induction by bacterial products, and its expression is augmented by gamma interferon. in this study, we performed a functional analysis of the murine and human p40 promoters in the murine macrophage cell line raw 264.7. transcription from the murine p40 promoter was strongly induced by lipopolysaccharide and heat-killed l ... | 1997 | 9234715 |
| ocular disease associated with silage feeding and listeria monocytogenes in fallow deer. | 1997 | 9234555 | |
| how the listeria monocytogenes acta protein converts actin polymerization into a motile force. | the acta protein is an essential determinant of pathogenicity that is responsible for the actin-based motility of listeria monocytogenes in mammalian cells and cell-free extracts. acta appears to control at least four functions that collectively lead to actin-based motility: (1) initiation of actin polymerization, (2) polarization of acta function, (3) transformation of actin polymerization into a motile force and (4) acceleration of movement mediated by the host protein profilin. | 1997 | 9234509 |
| bacterial phospholipases. | 1997 | 9234505 | |
| t-cell anergy induced by antigen presenting cells treated with the hemolysin of listeria monocytogenes. | antigen presenting cells (apc) that are infected with listeriolysin (llo) secreting listeria lack the ability to stimulate mhc class ii restricted t-cells by conventional antigens. similarly, t-cell activation by native proteins but not by peptides was inhibited upon pretreatment of apc with purified listeriolysin. the inhibition is due to an irreversible inactivation of t-cells that recognize antigen on infected or llo treated apc. inhibition was found to dominate over stimulation by peptides. ... | 1997 | 9232422 |
| effect of experimental infection with listeria monocytogenes on the development of pregnancy and on concentrations of progesterone, oestrone sulphate and 15-ketodihydro-pgf2 alpha in the goat. | the effect of listeria monocytogenes infection on hormone levels in pregnant goats was studied. four goats (group i) received an intravenous inoculation of a bacterial culture (type 1) on days 69-77 and another four goats (group ii) received a similar inoculation on days 105-106 of gestation. five non-inoculated goats were used as controls. plasma was analysed for progesterone, oestrone sulphate and 15-ketodihydro-pgf2 alpha. the status of the foetus was followed using real-time ultrasonography. ... | 1997 | 9231241 |
| expression of inducible nitric oxide synthase by macrophages in rat lung. | nitric oxide (no) is a short-lived free radical that is secreted by pulmonary macrophages (mø). an inducible isoform of no synthase (inos) catalyses the production of no and is activated by lipopolysaccharide and certain t-helper(h) 1 cytokines, including interferon-gamma and tnf-alpha. in the present study, inos+ interstitial cells were demonstrated in the alveolar wall of normal lewis rat lung. enzymatic digests of normal lung showed that approximately one third of pulmonary ed1+ interstitial ... | 1997 | 9230752 |
| a fatal case of listeria endocarditis in a man following his tending of goats suggests an epidemiological link which is not supported by the results. | a man died in endocarditis due to listeriosis in the late autumn. he had been looking after two goats during the summer. listeria monocytogenas was isolated from a rectal swab from one of the goats. the goat faeces isolate and the human blood isolate were of identical serovar. the two isolates, however, were shown to be different by multilocus electrophoretic enzyme analysis and ribotyping, as well as by biotyping. thus, these results do not support the hypothesis that the man was infected by th ... | 1997 | 9230676 |
| simple color tests based on an alanyl peptidase reaction which differentiate listeria monocytogenes from other listeria species. | the hydrolysis of dl-alanine-beta-naphthylamide and d-alanine-p-nitroanilide for identification of listeria spp. has been studied with 227 cultures. all species of listeria, except l. monocytogenes, hydrolyzed these substrates. the reactions were detected by simple chromogenic reactions and could substitute for the camp test. | 1997 | 9230403 |
| the human arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. | the arp2/3 protein complex has been implicated in the control of actin polymerization in cells. the human complex consists of seven subunits which include the actin related proteins arp2 and arp3, and five others referred to as p41-arc, p34-arc, p21-arc, p20-arc, and p16-arc (p omplex). we have determined the predicted amino acid sequence of all seven subunits. each has homologues in diverse eukaryotes, implying that the structure and function of the complex has been conserved through evolution. ... | 1997 | 9230079 |
| characterisation of the peptidoglycan hydrolases of listeria monocytogenes egd. | the peptidoglycan hydrolase profile of listeria monocytogenes egd has been characterised under a variety of environmental and physiological conditions, using renaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the profiles show activities ranging from 29 to 186 kda. the 186-kda enzyme was only observable under specific medium and aeration conditions. the enzyme activities show differential substrate specificity and sensitivity to incubation conditions. the peptidoglycan hydrola ... | 1997 | 9228781 |
| inter- and intraspecies comparison of the 16s-23s rrna operon intergenic spacer regions of six listeria spp. | the 16s-23s rrna intergenic spacer (igs) regions found in six listeria species were characterized. pcr amplification of the 16s-23s igs with a "generic primer" set generated products of about 340 bp (small) and 550 to 590 bp (large) with dna from all listeria strains tested. seven listeria monocytogenes serotype 4b strains and one l. monocytogenes serotype 4d strain also had an additional pcr product of ca. 360 bp. the 360-bp pcr product from one of these l. monocytogenes serotype 4b strains was ... | 1997 | 9226919 |
| listeria monocytogenes: a rare cause of pleural effusion in a patient with congestive cardiac failure. | we report the case of a 65 year old immunocompetent man with a listerial pleural effusion. infection of the pulmonary parenchyma and pleura with listeria monocytogenes has been reported in small numbers of immunocompromised patients but there have been only two previous reports of pulmonary listeria in non-compromised hosts. | 1997 | 9226774 |
| antibodies to listeria monocytogenes. | listeria monocytogenes is one of the leading foodborne pathogens and has been implicated in numerous outbreaks in the last 2 decades. immunocompromised populations are usually the most susceptible to listeria infections. although the pathogenic mechanism is a complex process, significant progress has been made in unravelling the mechanism in recent years. it is now clear that numerous extracellular and cell-associated proteins, such as internalin, listeriolysin, actin polymerization protein, pho ... | 1997 | 9226109 |
| a re-pcr method to distinguish listeria monocytogenes serovars. | strains (107) of l. monocytogenes were tested with a pcr-restriction enzyme analysis with two new original primers. a segment of 1395 bp containing the entire iap gene in l. monocytogenes was amplified by the pcr technique. the pcr product was cleaved with the restriction enzymes hindiii and rsai, and the fragments generated were separated by gel electrophoresis. two groups of serovars were obtained: one group contained serovars 1/2a and 1/2c, the other group contained serovars 1/2b, 3b and 4b. ... | 1997 | 9223613 |
| in-vitro activity of trovafloxacin against clinical bacterial isolates from patients with cancer. | the antibacterial activity of trovafloxacin was compared with that of ciprofloxacin, levofloxacin, ofloxacin, sparfloxacin and norfloxacin against bacterial isolates from patients with cancer. in general, the activity of trovafloxacin was comparable to that of ciprofloxacin, levofloxacin and sparfloxacin against most gram-negative isolates tested (minor differences in the activity of each agent against individual species were seen) and it was the most active agent tested against stenotrophomonas ... | 1997 | 9222065 |
| tnf-alpha-mediated expression of the receptor for anaphylatoxin c5a on neurons in experimental listeria meningoencephalitis. | the anaphylatoxin c5a has been implicated in the pathogenesis of bacterial meningitis as a potent mediator of inflammation in the subarachnoid space. we investigated the expression of the receptor for c5a (c5ar) in brains of mice with experimental listeria monocytogenes (lm) meningoencephalitis. in the course of the disease, infiltrating cells in the meninges and the ventricles were found to express c5ar mrna and protein. in the brain parenchyma, very low constitutive c5ar expression was seen on ... | 1997 | 9218605 |
| ligand recruitment by vinculin domains in transfected cells. | vinculin, a prominent protein component of microfilament-membrane attachment sites, consists of three major domains: an n-terminal, compact head and a c-terminal rod-like tail that are connected by a flexible, proline-rich hinge. in vitro, the protein has been shown to interact with numerous ligands, including other components of the microfilament system. to characterize the ligand recruitment ability of the different vinculin domains in a cellular environment, we used a novel approach of compri ... | 1997 | 9217322 |
| a heterogeneous population model for the analysis of bacterial growth kinetics. | a two-compartment, heterogeneous population model (hpm) was derived using the simulation software sb modelmaker to describe the growth of listeria monocytogenes in bacteriological media at 5-35 degrees c. the model assumed that, at time t = 0, the inoculum was distributed between two distinct compartments, non-growing and growing, and that growth could be described by four parameters: initial total cell population (n0), final maximum cell population (nmax), maximum specific growth rate (mu(max)) ... | 1997 | 9217107 |
| investigation of a listeriosis epizootic in sheep in new york state. | to investigate potential sources of an epizootic of listerial encephalitis, using molecular diagnostic and typing methods. | 1997 | 9215449 |
| the human lactobacillus acidophilus strain la1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. | the adhering human lactobacillus acidophilus strain la1 inhibits the cell association and cell invasion of enteropathogens in cultured human intestinal caco-2 cells (m. f. bernet, d. brassard, j. r. neeser, and a. l. servin, gut 35:483-489, 1994). here, we demonstrate that strain la1 developed its antibacterial activity in conventional or germ-free mouse models orally infected by salmonella typhimurium. we present evidence that the spent culture supernatant of strain la1 (la1-scs) contained anti ... | 1997 | 9212421 |
| the defined attenuated listeria monocytogenes delta mp12 mutant is an effective oral vaccine carrier to trigger a long-lasting immune response against a mouse fibrosarcoma. | listeria monocytogenes has been proposed as a carrier to elicit major histocompatibility complex class-i restricted immune responses able to protect against tumor challenge. in this study the properties of the attenuated l. monocytogenes delta mp12 mutant has been evaluated in vivo against a highly aggressive mouse fibrosarcoma which expresses beta-galactosidase (beta-gal) as a tumor-associated antigen (taa). immunization with the vaccine prototypes resulted in both elicitation of specific antib ... | 1997 | 9209512 |
| tap-dependent major histocompatibility complex class i presentation of soluble proteins using listeriolysin. | immunization of mice with mixtures of listeriolysin, a pore-forming hemolysin secreted by the pathogenic bacterium listeria monocytogenes, together with soluble ovalbumin, nucleoprotein of influenza virus, or beta-galactosidase of escherichia coli, resulted in strong cytotoxic cd8 t cell responses to each of the respective passenger proteins in vivo. also, the concomitant addition of either protein with listeriolysin to target cells elicited efficient sensitization of these cells which could be ... | 1997 | 9209484 |
| mr monitoring of a medically treated spinal cord abscess presumptively due to listeria monocytogenes. | 1997 | 9208269 | |
| profilin interacts with the gly-pro-pro-pro-pro-pro sequences of vasodilator-stimulated phosphoprotein (vasp): implications for actin-based listeria motility. | intracellular actin-based motility of listeria monocytogenes requires protein-protein interactions involving two different proline-rich sequences: first, the tightly bound bacterial surface protein acta uses its multiple oligoproline registers [consensus sequence = fe(d)fpppptd(e)e(d)] to tether vasodilator-stimulated phosphoprotein (vasp) to the bacterial surface; and second, vasp then deploys its own multiple gppppp (or gp5) registers to localize the actin-regulatory protein profilin to promot ... | 1997 | 9204886 |
| a comparison of immunomagnetic and surface adhesion immunofluorescent techniques for the rapid detection of listeria monocytogenes and listeria innocua in meat. | an immunomagnetic immunofluorescent method was investigated for the rapid detection of listeria monocytogenes and listeria innouca. this technique involved enrichment of the suspect sample at 30 degrees c overnight. listeria monocytogenes cells were isolated from the enriched sample using immunomagnetic separation and listeria were subsequently visualized using an immunofluorescent microscopy technique. this technique was used in the detection of listeria cells from pure culture, inoculated beef ... | 1997 | 9203399 |
| research note: in vitro inhibition of listeria monocytogenes growth by veillonellae cultures grown on tartrate media. | veillonellae cultures were grown on agar media supplemented with tartrate and examined for inhibitory effects on the growth of listeria monocytogenes. veillonellae cultures grown on media supplemented with 0 or 50 mmol l-1 of tartrate did not inhibit the growth of l. monocytogenes; however, veillonellae grown on media supplemented with 100, 150 or 200 mmol l-1 of tartrate did inhibit the growth of l. monocytogenes. the inhibition of the growth of l. monocytogenes by the veillonellae was correlat ... | 1997 | 9202444 |
| fate of low temperature and acid-adapted yersinia enterocolitica and listeria monocytogenes that contaminate lactic acid decontaminated meat during chill storage. | pathogens found in the environment of abattoirs may become adapted to lactic acid used to decontaminate meat. such organisms are more acid tolerant than non-adapted parents and can contaminate meat after lactic acid decontamination (lad). the fate of acid-adapted yersinia enterocolitica and listeria monocytogenes, inoculated on skin surface of pork bellies 2 h after lad, was examined during chilled storage. lad included dipping in 1%, 2% or 5% lactic acid solutions at 55 degrees c for 120 s. lad ... | 1997 | 9202443 |
| bioactivity of selected plant essential oils against listeria monocytogenes. | ninety-three different commercial essential oils were screened for activity against 20 listeria monocytogenes strains in vitro and the results correlated against the actual chemical composition of each oil. there was a substantial difference in the activity between different essential oils as expected, but there was also a difference in activity between different samples of the same essential oil. strong anti-listeria activity was often correlated with essential oils containing a high percentage ... | 1997 | 9202441 |
| il-4 secretion by cd4+ nk1+ t cells induces monocyte chemoattractant protein-1 in early listeriosis. | il-4 is a major promotor of th2 differentiation and an antagonist of ifn-gamma production. although experimental listeriosis is characterized by a th1 response, il-4-producing cells were detected in spleens of mice promptly after listeria monocytogenes infection. we identified this early il-4 as inducer of the chemokine, monocyte chemoattractant protein-1 (mcp-1), which mainly attracts monocytes/macrophages, but not neutrophils. mcp-1-secreting cells were demonstrable in spleens of mice infected ... | 1997 | 9200431 |
| effects of ph and salinity on the antimicrobial properties of clavanins. | clavanins are histidine-rich, amidated alpha-helical antimicrobial peptides that were originally isolated from the leukocytes (hemocytes) of a tunicate, styela clava. the activities of clavanin a amide and clavanin a acid against escherichia coli, listeria monocytogenes, and candida albicans were substantially greater at ph 5.5 than at ph 7.4. in contrast, clavanin ak, a synthetic variant of clavanin a acid containing 4 histidine-->lysine substitutions exerted substantial activity at both ph 7.4 ... | 1997 | 9199465 |
| utilization of iron-catecholamine complexes involving ferric reductase activity in listeria monocytogenes. | listeria monocytogenes is a ubiquitous potentially pathogenic organism requiring iron for growth and virulence. although it does not produce siderophores, l. monocytogenes is able to obtain iron by using either exogenous siderophores produced by various microorganisms or natural catechol compounds widespread in the environment. in the presence of tropolone, an iron-chelating agent, growth of l. monocytogenes is completely inhibited. however, the growth inhibition can be relieved by the addition ... | 1997 | 9199450 |
| ribotypes and virulence gene polymorphisms suggest three distinct listeria monocytogenes lineages with differences in pathogenic potential. | a total of 133 listeria monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes hly, acta, and inla to uncover linkages between independent phylogenetic and specific virulence markers. pcr-restriction fragment length polymorphisms revealed 8 hly, 11 inl4, and 2 acta alleles. the combination of these virulence gene alleles and ribotype patterns separated l. monocytogenes into three distinct lineages. while distinct hly and inla alleles were generally fo ... | 1997 | 9199440 |
| listeria monocytogenes-induced liver abscess in pregnancy. | 1997 | 9197456 | |
| mice lacking reduced nicotinamide adenine dinucleotide phosphate oxidase activity show increased susceptibility to early infection with listeria monocytogenes. | mice lacking the gp91 protein of reduced nicotinamide adenine dinucleotide phosphate oxidase showed heightened susceptibility to listeria infection. the enhanced susceptibility was noted 2 days after infection, the usual peak time of the neutrophil-dependent lesion in the liver. infected gp91phox -/- mice had an increase of approximately 30-fold in listeria in the liver and an increase in the number of microabscesses. the study of the gp91phox mice underscores the early role of the neutrophil an ... | 1997 | 9190903 |
| inhibition of gamma interferon synthesis by catecholamines. | in vitro incubation of listeria monocytogenes immune spleen cells in the presence of the catecholamines epinephrine or norepinephrine inhibited the gamma interferon (ifn-gamma) synthesis induced by the mitogen pha, in a manner that appeared to be concentration dependent. moreover, the inhibitory effect of both catecholamines epinephrine and norepinephrine on the synthesis of ifn-gamma was prevented by incubating immune spleen cells in the presence of propranolol, a beta adrenergic antagonist age ... | 1997 | 9184628 |
| persistent production of interferon-gamma (ifn-gamma) and il-12 is essential for the generation of protective immunity against listeria monocytogenes. | ifn-gamma and il-12 are believed to be important in the host defence against listeria infection in mice. however, the relationship between these two cytokines and generation of protective immunity remains poorly understood. in the present study, it was found that at least 4 days of immunizing infection were required for the generation of protective immunity against l. monocytogenes. protective immunity was generated only by immunizing infection with virulent strain. even repeated injections of a ... | 1997 | 9182892 |
| proteolytic pathways of activation and degradation of a bacterial phospholipase c during intracellular infection by listeria monocytogenes. | listeria monocytogenes is a facultative intracellular bacterial pathogen that spreads cell to cell without exposure to the extracellular environment. bacterial cell-to-cell spread is mediated in part by two secreted bacterial phospholipases c (plc), a broad spectrum plc (pc-plc) and a phosphatidylinositolspecific plc (pi-plc). pi-plc is secreted in an active state, whereas pc-plc is secreted as an inactive proenzyme (propc-plc) whose activation is mediated in vitro by an l. monocytogenes metallo ... | 1997 | 9182669 |
| dietary fish oil enhances circulating interferon-gamma in mice during listeriosis without altering in vitro production of this cytokine. | the objective of this study was to investigate the impact of feeding mice a diet rich in n-3 polyunsaturated fatty acids (pufa) from fish oil on the interferon-gamma (ifn-gamma) response during an active infection with listeria monocytogenes. weanling female c3h/hen mice were fed experimental diets containing 20% by weight one of the following fats: soybean oil, lard, or a mixture of menhaden fish oil and corn oil (17:3, w/w). after 4 weeks, mice were injected with 10(5) live l. monocytogenes, a ... | 1997 | 9181465 |
| nosocomial infections by listeria monocytogenes: analysis of a cluster of septicemias in immunocompromised patients. | from december 1994 to november 1995 an unusual accumulation of listeria infections occurred at the university hospital hamburg-eppendorf, germany. eleven immunosuppressed patients from different departments developed septicemia due to listeria monocytogenes during hospitalization. in a retrospective study, serotyping and pulsed-field gel electrophoresis revealed that six isolates were identical or genetically related. four of them had been isolated from renal transplant recipients. listeria mono ... | 1997 | 9181378 |
| ultrastructural localization of acid phosphatase in some bacteria, after treatment with lubrol w1. | the ultracytochemical localization of acid phosphatase from some bacteria (listeria monocytogenes, salmonella typhimurium, pseudomonas pseudomallei and pseudomonas aeruginosa) was dependent on the changes in the lipoprotein content of the membranes as a result of the action of the lubrol w1. | 1996 | 9178530 |
| optimization of haemolysis in enhanced haemolysis agar (eha)--a selective medium for the isolation of listeria monocytogenes. | the presence of listeria monocytogenes in enrichment media can be masked by faster growth of other listeria spp. therefore, enhanced haemolysis agar (eha) is a good alternative for another isolation media, because the presence of a few l. monocytogenes colonies can be detected in a majority of colonies of other listeriae on the basis of haemolysis. in this study the haemolysis reaction in eha was optimized. in a collaborative study using reference samples, no significant differences in counts on ... | 1997 | 9172448 |
| the use of the bacteriocin, nisin, as a preservative in ricotta-type cheeses to control the food-borne pathogen listeria monocytogenes. | the efficacy of nisin to control the food-borne pathogen listeria monocytogenes in ricotta-type cheeses over long storage (70 d) at 6-8 degrees c was determined. cheeses were prepared from unpasteurized milk by direct acidification with acetic acid (final ph 5.9) and/or calcium chloride addition during heat treatment. nisin was added in the commercial form of nisaplin pre-production to the milk. each batch of cheese was inoculated with 10(2)-10(3) cfu g-1 of a five-strain cocktail of l. monocyto ... | 1997 | 9172439 |
| prevalence of listeria sp. in droppings from urban rooks (corvus frugilegus). | droppings from 112 urban rooks (corvus frugilegus) were cultured for the presence of listeria sp. overall, 46% of rooks sampled harboured one or more listeria species. of all birds examined, 33%, 24% and 8%, respectively, were infected with listeria monocytogenes, listeria innocua and listeria seeligeri. differentiation of l. monocytogenes and l. seeligeri carried out by several phenotypic typing methods proved the diversity of strains and the major role of rooks which widely contribute to sprea ... | 1997 | 9172407 |
| expanded models for the non-thermal inactivation of listeria monocytogenes. | previously developed four-variable response surface models for describing the effects of temperature, ph/lactic acid, sodium chloride and sodium nitrite on the time to achieve a 4-log, non-thermal inactivation (t4d) of listeria monocytogenes in aerobic, acidic environments were expanded to five-variable models that distinguish the effects of ph and acidulant concentration. a total of 18 new variable combinations were evaluated and the inactivation kinetics data appended onto a consolidation of t ... | 1997 | 9172398 |
| isolation and characterization of a listeria monocytogenes mutant strain hyperproducing virulence factors. | a mutant strain characterized by hyperproduction of a number of cell wall and supernatant proteins was isolated after n'methyl-n'-nitro-n-nitrosoguanidine treatment of listeria monocytogenes strain nctc10527. several of these proteins were identified as virulence factors. when a wild-type strain was grown in the presence of activated charcoal it was shown to exhibit the same protein pattern as the isolated mutant. | 1997 | 9170261 |
| broad-spectrum antimicrobial activity of human intestinal defensin 5. | defensins are antibiotic peptides expressed in human and animal myeloid and epithelial cells. due to the limited availability of natural peptides, the properties of human epithelial defensins have not been studied. we assayed the microbicidal activity of recombinant human intestinal defensin 5 (rhd-5) in the presence of salt (o to 150 mm nacl) with varied ph (ph 5.5 to ph 8.5) and trypsin (25 and 250 microg/ml). rhd-5 exhibits microbicidal activity against listeria monocytogenes, escherichia col ... | 1997 | 9169780 |
| listeriosis after fludarabine treatment for chronic lymphocytic leukemia. | the authors report a case of listeria monocytogenes septicemia in a patient with advanced cll after a single course of fludarabine, without any other immunosuppressive therapy e.g. corticosteroids. the immunosuppressive action of fludarabine in patients who are already severely immunosuppressed must be considered from a diagnostic and therapeutic point of view. listeriosis and other opportunistic infections, like pneumocystis carinii pneumonia, have been reported during and after treatment with ... | 1997 | 9168306 |
| listeria monocytogenes infection of cultured endothelial cells stimulates neutrophil adhesion and adhesion molecule expression. | microbial infection of the endothelium with the resultant up-regulation of adhesion molecule expression and stimulated leukocyte adhesion to endothelial cells can promote an inflammatory response. previous work demonstrated that listeria monocytogenes can replicate within cultured endothelial cells; thus, we tested whether l. monocytogenes infection of huvec stimulated an inflammatory phenotype on these cells. infection with 10(4) cfu of bacteria increased neutrophil adhesion to huvec 40-fold an ... | 1997 | 9164950 |
| impaired macrophage listericidal and cytokine activities are responsible for the rapid death of listeria monocytogenes-infected ifn-gamma receptor-deficient mice. | ifn-gamma receptor-deficient (ifn-gammar -/-) mice were used to study the innate immune responses during infection with listeria monocytogenes. mutant mice were unable to limit bacterial growth and died of sepsis even with an infection dose of 70 listeria. at day 2, they showed an exacerbated listeriosis and mice succumbed to infection before the onset of an effective specific immunity, demonstrating a defective innate immunity. recruitment and extravasation of phagocytic cells to infected organ ... | 1997 | 9164949 |
| multifocal defects in immune responses in relb-deficient mice. | mice with a targeted disruption of the rel/nuclear factor-kappab family member relb develop a complex inflammatory phenotype, myeloid hyperplasia, and splenomegaly due to extramedullary hemopoiesis. in this work, we report that relb-deficient mice, in addition to the pathologic changes, were highly susceptible to infection by the facultative intracellular bacterium listeria monocytogenes. relb binds transcriptionally active kappab motifs in the tnf-alpha promoter in normal cells, and in vitro st ... | 1997 | 9164938 |
| detection and identification of listeria monocytogenes from milk and cheese by a single-step pcr. | primers of iap gene were used as a target to develop a pcr technique for detecting listeria monocytogenes in milk and cheese. the pcr technique gives good results in the detection of listeria monocytogenes either in artificially or naturally contaminated foodstuffs and has a high sensitivity and specificity. application of this rapid diagnostic tool could provide further information about the spread of l. monocytogenes in milk and cheese. | 1997 | 9163724 |
| serological diagnosis of bovine, caprine, and ovine mastitis caused by listeria monocytogenes by using an enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay for detecting listeria monocytogenes antibodies in bovine (n = 35), caprine (n = 27), and ovine (n = 30) milk samples was evaluated by comparison with bacteriological examination. microtiter plates were coated with proteins obtained from culture supernatant, and antibodies were revealed with a monoclonal antibody able to react with the immunoglobulins belonging to the three animal species. the arithmetic mean optical density (od) of milk samples infected with ... | 1997 | 9163495 |