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isolation of campylobacter jejuni in hunan, the people's republic of china: epidemiology and comparison of chinese and american methodology.the incidence of infection with campylobacter jejuni was determined in individuals with and without diarrheal disease seen at a hospital in changsha, china. stool specimens were cultured by two methods: one developed by the chinese and a second control method used commonly in the united states. among people with diarrhea, 18.7% of the 48 children and 8.7% of 104 adults were infected with campylobacter jejuni. in the group without diarrhea, 8.6% of 105 children but none of 76 adults tested had th ...19863522087
enrichment for detection of campylobacter jejuni.gifu anaerobe-modified semisolid medium supplemented with vancomycin, polymyxin b, trimethoprim, and amphotericin b can serve as an efficient enrichment for the recovery of campylobacter jejuni under aerobic conditions. it is superior to the direct plating method in its recovery of very small numbers of c. jejuni. blood supplementation, agitation, and elevated temperatures are not required for this improved recovery.19863519661
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...19863517036
western blot analysis of intestinal secretory immunoglobulin a response to campylobacter jejuni antigens in patients with naturally acquired campylobacter enteritis.secretory immunoglobulin a (siga) response at the intestinal mucosa is a primary defense against enteric infections. we sought to determine which antigens of campylobacter jejuni outer membranes elicited siga responses in 8 patients with naturally acquired campylobacter enteritis using western blot analysis of fecal extracts. naturally acquired campylobacter infection elicited an siga response in 7 of 8 patients. of these 7 patients, 5 had campylobacter-specific siga titers of 1:16 and two had t ...19863514359
extraintestinal campylobacter jejuni and campylobacter coli infections: host factors and strain characteristics.to determine whether extraintestinal isolates of campylobacter jejuni and campylobacter coli are the consequence of unusual host or bacterial characteristics, we studied clinical and bacteriologic features of 24 extraintestinal infections. common serotypes and auxotypes were present among the extraintestinal isolates. gastrointestinal isolates were more susceptible to normal human serum than were the systemic isolates; however, the ranges overlapped considerably. predispositions to systemic spre ...19863512731
[campylobacter jejuni in food originating from poultry]. 19873505671
causes of death in diarrhoeal diseases after rehydration therapy: an autopsy study of 140 patients in bangladesh.the cause of death (besides dehydration) for 140 diarrhoeal patients who died in hospital following rehydration was determined by autopsy examination. children under 5 years comprised 74% of the patients. diarrhoeal pathogens were identified as shigella spp. in 27%, enterotoxigenic escherichia coli in 17%, entamoeba histolytica in 16%, campylobacter jejuni in 12%, salmonella spp. in 4%, vibrio cholerae in 4%, and giardia lambliain 4% of cases. the most frequent underlying causes of death were co ...19873499247
susceptibility of clinical isolates of campylobacter jejuni to twenty-five antimicrobial agents.the in-vitro activity of 25 antimicrobial agents against 113 to 161 clinical isolates of campylobacter jejuni was tested by an agar dilution method. all strains were susceptible to the four aminoglycosides tested, to imipenem, chloramphenicol and norfloxacin. one strain (0.6%) was resistant to each of the following antibiotics: nalidixic acid, erythromycin and clindamycin. the frequency of tetracycline, metronidazole and cotrimoxazole resistance among our isolates was 14.5%, 56.4% and 96% respec ...19863493235
early treatment with erythromycin of campylobacter jejuni-associated dysentery in children.to evaluate the efficacy of early treatment with erythromycin on the duration of fecal excretion and of diarrhea associated with campylobacter jejuni, 170 patients, age 3 to 60 months, were randomly assigned in a double-blind fashion to receive either erythromycin ethyl succinate or placebo immediately after being seen at cayetano heredia hospital because of acute dysentery. the groups' pretreatment characteristics were comparable. of the 30 patients with stools positive for c. jejuni, 12 were i ...19863488385
in vitro study of the antibacterial activity of ofloxacin against recent clinical isolates.the in vitro activity of ofloxacin, a new broad-spectrum antimicrobial agent, was studied by a standardized single disc method. a total of 990 clinical isolates were tested, including 20 strains of anaerobic bacteria. ofloxacin was highly active against 683 strains (70.41%), had intermediate activity against 109 (11.23%) and had no activity against 178 (18.35%). ofloxacin was highly active against e. coli, klebsiella sp., citrobacter sp., proteus mirabilis, proteus morganii, salmonella sp., camp ...19873476290
the incidence of campylobacter jejuni/coli in infants acute diarrhoeal disease. 19873455653
immunoblot and enzyme-linked immunosorbent assays of campylobacter major outer-membrane protein and application to the differentiation of campylobacter species.the major outer-membrane protein (momp) from campylobacter jejuni nctc 11168 was purified by solubilization in triton x-100. whole-cell proteins of campylobacter species and the momp were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linked immunoblot assay (elisa) analyses using polyclonal antiserum to the c. jejuni momp. purified momp from c. jejuni nctc 11168 contained a single major protein of 46 kda. whole-cell ...19873453900
molecular cloning of a species-specific dna probe for campylobacter jejuni.conventional procedures for isolating and identifying campylobacter jejuni are cumbersome and time-consuming. a simpler approach would be to use dna probes to identify these organisms. to obtain such probes we cloned chromosomal dna from c. jejuni into the lambda replacement vector embl 4. recombinant phages were screened for c. jejuni-specific inserts by dna hybridization using chromosomal dna from either c. jejuni or c. coli which had been radioactively labelled with 32p. by this means, recomb ...19873453899
[bacteriostatic and bactericidal activity, resistance and tolerance of 16 strains of thermophilic campylobacter to 12 antibiotic drugs].the majority of gastrointestinal infections due to "thermophilic" campylobacter is self limiting and does not need antibiotic treatment. anyhow there are some serious cases (sepsis, persistent and relapsing gastroenteritis, severe immunodeficient patients) which require appropriate therapy. the susceptibility of 15 strains of campylobacter jejuni and of 1 strain of c. coli, isolated from patients with acute gastroenteritis, has been studied against 12 antibiotics with the broth microdilution met ...19873453149
[effect of the components of preservative mixtures on the growth of campylobacter jejuni]. 19873451353
protection against campylobacter jejuni infection in suckling mice by anti-flagellar antibody.we obtained two monoclonal antibodies of igm class and iga class of immunoglobulin prepared from mouse spleen cells immunized with crude flagellar preparation, and a polyclonal antibody raised against purified flagellin monomer of campylobacter jejuni in a rabbit. the specificity of the reaction of these antibodies for flagellar filament was confirmed by western blotting and by immunoelectron microscopy. these antibodies caused agglutination of the bacteria and inhibited the motility of the bact ...19873451028
campylobacter jejuni and yersinia enterocolitica in urban and rural nigerian school children. 19873450725
antimicrobial susceptibilities of diarrhoeagenic campylobacter jejuni and yersinia enterocolitica isolated in nigeria. 19873450406
[studies on the adhesion of campylobacter jejuni/coli to hela cells]. 19873449423
childhood enteritis: a community study.the population aged 0-12 years living in the area of an italian local health unit (about 8,800 children) was studied for one year in order to estimate the overall incidence rate of enteritis and the incidence rates of diarrheas from bacterial agents. all children complaining of acute diarrhea and seen by the pediatric practices of the study area were recorded by date, age, and sex; the microbiological study of stools was performed for a sample of patients. all hospitalized cases of childhood ent ...19873449099
prevalence of campylobacter jejuni in eggs and poultry meat in new york state.the presence of campylobacter jejuni was tested for but not isolated from any of 276 eggs sampled from 23 egg farms in new york state. the presence of c. jejuni was evaluated in broilers, kosher broilers, spent layers, peking ducks, and turkeys. four of five poultry dressing plants tested showed positive growth of c. jejuni on the 25-carcass samples at various stages of processing. twenty to 100% of live birds sampled contained c. jejuni on the skin but 90 to 100% were contaminated after scaldin ...19873447135
[effects of disinfectants on campylobacter jejuni].the effect of three disinfectants on campylobacter jejuni strains isolated from poultry feces was investigated. two-fold dilutions of ethyl alcohol, formalin and benzalkonium chloride were contacted with bacterial suspension in 1 to 60 minutes. seventy per cent of ethyl alcohol and 2.5 per cent of formalin killed all bacteria within one minute. benzalkonium chloride (1:50.000) was effective to all bacteria within 5 minutes. under the conditions of this study, the recommended standard concentrati ...19873447021
campylobacter jejuni infection in a general hospital. 19873446687
[intestinal infections of campylobacter jejuni etiology in infants in the first trimester of life]. 19873445610
[survival of campylobacter jejuni in water with added potassium nitrate]. 19873445090
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.19873443752
serological investigations on the cause of diarrhoea on the cape verde islands.in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9.19873443746
frequency of campylobacter jejuni and other agents of acute diarrhea in venezuelan children. 19873442715
serogroups and biotypes of human strains of campylobacter jejuni and campylobacter coli isolated in france.the lior schemes were used for biotyping and serogrouping campylobacter strains isolated during a three year period in bordeaux, france. of the 226 strains tested, 176 were campylobacter jejuni and 50 campylobacter coli. campylobacter jejuni biotype i was the most prevalent (48.2%). biotypes iii and iv of campylobacter jejuni were rare (3.9% and 1.3% respectively). serogroup 4 (17.7%) was the most common serogroup followed by serogroups 46 (11.0%), 29 (10.1%), 9 and 36 (7.9%). eight serogroups c ...19873440456
antibody response in patients infected with campylobacter jejuni/coli assayed with a complement fixation test and a digelisa method.two methods for the detection of antibodies against campylobacter jejuni/coli, a complement fixation test (cft) and a dig-elisa method, were tested against a panel of single sera from 36 coproculture positive patients. twenty-five were positive in cft compared to 31 in the other system. twenty-three out of 25 cft positive sera were positive in the elisa method. seven sera were positive only for iga antibodies thus negative in the cft. cft may be useful for the detection of igg/igm antibodies aga ...19873438028
susceptibility of campylobacter species to metronidazole, its bioactive metabolites and tinidazole.the susceptibilities of 115 campylobacters, belonging to five species, to metronidazole, its principal oxidative metabolites and tinidazole were determined by an agar dilution method. the activities of metronidazole and tinidazole were comparable. the hydroxy metabolite was more active than the parent drug against most species, particularly campylobacter jejuni and campylobacter pyloridis. although the acid derivative was less active against c. jejuni and campylobacter coli, it demonstrated an e ...19873436679
dna probes for identification of tetracycline resistance genes in campylobacter species isolated from swine and cattle.tetracycline-resistant strains of campylobacter jejuni and campylobacter coli from swine and cattle colons were isolated and characterized by hybridization with dna probes. a probe consisting of the 1.8-kilobase (kb) hincii fragment from pua466 was highly specific for the detection of tetracycline resistance (tcr) in c. jejuni and c. coli. the 5-kb tetm dna probe from streptococcus agalactiae plasmid pji3 which has homology with the 1.8-kb hincii fragment from pua466 could also be used to detect ...19873435114
laboratory and clinical evaluation of isolation media for campylobacter jejuni.six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...19873429621
yoghurt: an unlikely source of campylobacter jejuni/coli.knowledge of the relative insensitivity of campylobacter jejuni to moderately acid environments prompted us to study its survival in different batches of yoghurt of ph range 4.2-5.3 and the role of organic or inorganic acid in the die-off of this pathogen. none of the 11 strains of c. jejuni or c. coli survived more than 25 min in yoghurt. suspecting that this rapid die-off cannot be accounted for by the ph of the yoghurt we compared the survival rates of c. jejuni in milk, whose ph had been adj ...19873429355
[gastroenteritis caused by campylobacter jejuni in childhood. study of 100 cases].authors report results of the study on a series of 100 pediatrics patients with campylobacter gastroenteritis whose ages were between 1 month and 14 years. incidence of campylobacter jejuni isolation among 2,733 samples of diarrheal stools, examined during a period of 3 years, was 146 cases (5.03%). several epidemiological, clinical and therapeutic aspects of the infection are analysed. campylobacter jejuni is an important enteric pathogen in infancy, and clinical symptoms due to infection are d ...19873426015
studies on intestinal mucosal immunity in mice orally immunized with killed campylobacter jejuni. 19873425466
campylobacter jejuni enteritis in a premature neonate.we have described the first case of campylobacter jejuni enteritis in a premature neonate, whose clinical course was characterized by a self-limited bloody diarrhea accompanied by metabolic acidosis and poor weight gain. the importance of this organism in causing preterm labor in mothers and enteritis in premature neonates awaits systematic study.19873423907
campylobacter jejuni/coli in commercially reared beagles: prevalence and serotypes.a 5 year longitudinal study involving 187 commercially reared beagles from three suppliers was undertaken to determine prevalence and serotypes of campylobacter jejuni and c. coli. campylobacter jejuni or c. coli was isolated from the feces in 62 of 177 asymptomatic beagles and 8 of 10 dogs with diarrhea for an overall prevalence of 37%. a total of 36 isolates were serotyped on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes isolated from human ...19883411913
occurrence of campylobacter jejuni in free-living wild birds from japan.campylobacter jejuni was isolated from 44 of 313 free-living birds from japan. the highest isolation rate was found in 30 of 87 (34%) crows (corvus levaillanti and corvus corone), followed by 2 of 10 (20%) blue magpies (cyanopica cyanus), 5 of 35 (14%) gray starlings (sturnus cineraceus), 2 of 16 (13%) domestic pigeons (columbia livia domestica), 4 of 36 (11%) bulbuls (hypsipetes amaurotis), and 1 of 62 (2%) eastern turtledoves (streptopelia orientalis). one-fourth of the contents of the crop an ...19883411703
antibody response to campylobacter coli in children during intestinal infection and carriage.in the central african republic, etiological studies of diarrhea have shown that campylobacter coli accounts for almost 40% of campylobacter enteric isolations. this prompted us to investigate the antibody response to c. coli infection in children. as expected from the literature on campylobacter jejuni infections, our results show that both infection and carriage elicited antibodies against glycine-extracted membrane antigens, flagella, and cholera toxin. the human antibody response to c. coli ...19883410956
characterization of campylobacter strains isolated in bangladesh from different sources.campylobacters have been isolated in bangladesh from patients with gastroenteritis, from healthy individuals and animals. a total of 180 campylobacter isolates, 100 from patients with gastroenteritis reporting to international centre for diarrhoeal disease research, bangladesh hospitals, 50 from asymptomatic carriers and the other 30 from domestic animals of the index cases have been characterized following standard procedures available. all isolates tested have been confirmed as campylobacter j ...19883404566
a prospective study of acute idiopathic neuropathy. ii. antecedent events.the incidence of antecedent events and serological evidence of preceding infection were studied in 100 patients with acute idiopathic neuropathy and age and sex matched control subjects in south-east england. symptoms of respiratory infections occurred within one month before onset of neuropathic symptoms in 38% of patients and 12% of controls (p less than 0.001) and symptoms of gastrointestinal infections in 17% of patients and 3% of controls (p less than 0.005). immunisations, insect bites and ...19883404161
cefetamet pivoxil: bacteriostatic and bactericidal activity of the free acid against 355 gram-negative rods.the in vitro activity of the free acid of cefetamet pivoxil (ro 15-8075) was tested against 355 clinical isolates, namely enteropathogenic bacteria, glucose non-fermentative gram-negative rods (excluding pseudomonas aeruginosa) and legionella pneumophila. ceftriaxone was included in the study as reference compound. although the free acid of the orally active cephalosporin was generally weaker than ceftriaxone, it inhibited 88.2% and 94.5% of enterobacteriaceae and vibrionaceae at a concentration ...19883403040
colonization characteristics of campylobacter jejuni in chick ceca.we report our findings on several parameters influencing cecal colonization of chickens by campylobacter jejuni. thirty-five colony-forming units (cfu) of a composite culture of c. jejuni colonized the ceca of one-half of the newly hatched chicks challenged by oral gavage. a challenge dose of 3500 cfu/chick consistently colonized the ceca of all chicks challenged. challenge doses of approximately 10(5) cfu of c. jejuni per chick resulted in consistent cecal colonization, regardless of whether th ...19883401176
cross-protection of infant mice against intestinal colonisation by campylobacter jejuni: importance of heat-labile serotyping (lior) antigens.an association of the heat-labile antigens detected by the lior serotyping scheme with ability to protect infant mice against gastrointestinal colonisation with campylobacter jejuni has been established. overall, 39 (57%) of 68 infant mice challenged with a heterologous strain of the same lior serotype as the vaccine strain were protected, compared with 40 (85%) of 47 infants protected against a homologous challenge. in contrast, none of the infant mice challenged with a strain carrying the same ...19883398033
purification and antigenic analysis of flagella of campylobacter jejuni.the flagella of campylobacter jejuni strain fum158432 were purified and a flagellin preparation consisting of only a single peptide of 63,000 daltons was obtained. the peptide of 92,000 daltons usually associated with a flagellar preparation was shown to be a peptide derived from the hook region. antiserum was prepared by immunizing a rabbit with the flagellin preparation. the reaction of the antiserum was found to be highly specific for the flagellar filament by immunoelectron microscopy and fo ...19883393098
[biotyping and serotyping and plasmid analysis of campylobacter jejuni/coli]. 19883391596
intestinal colonization of campylobacter jejuni in young japanese quails (coturnix coturnix japonica). 19883386114
intestinal colonization of campylobacter jejuni in chickens. 19883386104
enterotoxigenicity of chicken isolates of campylobacter jejuni in ligated ileal loops of rats.ligated ileal loops in rats of the charles-foster strain, weighing 200-250 g and aged 7-8 months, provided a sensitive and reproducible means of testing the enterotoxigenicity of campylobacter jejuni. all of 16 chicken isolates caused fluid accumulation comparable to that produced by toxigenic vibrio cholerae o1, strain 569b. however, 11 of the isolates required one to three consecutive passages through the rat gut before doing so. the amount of fluid produced increased after each passage. of th ...19883385766
improved blood free selective medium for isolating campylobacter jejuni from faecal specimens. 19883385002
response of campylobacter jejuni to combinations of ferrous sulphate and cadmium chloride.on mueller hinton (mh) agar, campylobacter jejuni showed 20.0 and 30.9 mm zones of inhibition surrounding discs impregnated with 2.5 and 20 micrograms cdcl2 respectively. the minimal inhibitory concentration (mic) ranged from 0.64 to 3.2 micrograms cdcl2/ml of mh agar for four c. jejuni strains. in the presence of 23 micrograms feso4/ml of mh the mic increased to a range of 1.5-5.4 micrograms cdcl2/ml of mh. moreover, the numbers of colonies present on mh supplemented with feso4 were greater tha ...19883384765
fisher's syndrome associated with campylobacter jejuni infection.a patient developed typical features of fisher's syndrome after an infection with campylobacter jejuni as ascertained by positive serology both in the blood and spinal fluid. this may be the first case of this association described.19883383912
[biotyping and plasmid analysis of campylobacter jejuni/coli and their preliminary application in epidemiological studies]. 19883383232
migratory birds of central washington as reservoirs of campylobacter jejuni.migratory ducks, canada geese, and sandhill crane from the pacific north american flyway have been screened for campylobacter spp. samples (298) from these birds were examined and the incidence of campylobacter spp. in the samples were as follows: sandhill crane (grus canadensis tabida), 81%; ducks (aythya collaris, anas carolinensis, aythya americana, and anas platyrhynchos), 73%; and canada geese (branta canadensis), 5%. all isolates were identified as campylobacter jejuni. to our knowledge th ...19883378202
campylobacter jejuni infections in gnotobiotic pigs.at 3 days of age, 12 gnotobiotic pigs were inoculated orally with broth cultures of campylobacter jejuni. one pig was euthanatized and evaluated each day for 12 days. in the cecum and colon, there was diffuse edema, neutrophilic infiltration, and sloughing of epithelial cells from the mucosa on postinoculation days (pid) 2 through 5. dysplastic colonic crypt epithelial cells were observed in the submucosa of the colon on pid 5 through 12. curved, rod-shaped bacteria were detected on the surface ...19883377304
the mode of spread of campylobacter jejuni/coli to broiler flocks. 19883376623
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
losses related to the ingestion of lincomycin-medicated feed in a range sheep flock.because of an episode of abortion caused by a tetracycline-resistant campylobacter jejuni, lincomycin was mixed into a barley pellet and fed to 3,000 range ewes at a dosage of 225 mg/ewe/day. after the second feeding, a number of ewes were anorectic and diarrheic, and 6 were dead. necropsies revealed congestion and hemorrhage of the entire gastrointestinal tract. the feeding of the medicated pellets was discontinued immediately. sick ewes were treated with antihistamines and corticosteroids, but ...19883372336
chemotactic behavior of campylobacter jejuni.the chemotactic behavior of campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. l-fucose was the only carbohydrate and l-aspartate, l-cysteine, l-glutamate, and l-serine were the only amino acids producing a chemotactic (positive) response. several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were ...19883372020
cryopreservation studies of campylobacter.seven strains of campylobacter fetus ss. fetus, one of campylobacter fetus ss. venerealis, and one of campylobacter jejuni were preserved using a variety of cryopreservation methods. organisms were frozen to -150 degrees c in a liquid nitrogen refrigerator, in the freezer compartment of a refrigerator (-20 degrees c), and in a mechanical freezer (-65 degrees c). in the latter two cases, viabilities of the organisms were compared after being frozen in brucella albimi broth and 10% glycerol. viabi ...19883371059
campylobacter diarrhea and guillain-barré syndrome.four of 106 consecutive patients with acute guillain-barré syndrome had preceding bacterial enteritis caused by campylobacter jejuni. one had a severe illness with axonal damage and poor outcome; three had typical courses with good recovery. preceding illnesses in the other 102 patients were the following: diarrhea in nine (with negative stool cultures in five), upper respiratory tract infections in 46, hepatitis in six, other infectious illnesses in eight, and none in 33. eight patients previou ...19883369973
guillain-barré syndrome following campylobacter jejuni enteritis. 19883369964
experimental infection of dogs with campylobacter jejuni.three groups of conventional puppies were inoculated orally with campylobacter jejuni biotype 2 which had been isolated from the small intestine of a dog with enteritis. mild enteric disease was observed in one group. there was superficial intestinal colonization by the organism but penetration of intestinal epithelial cells was not apparent. c jejuni was isolated from the blood and viscera of inoculated dogs which showed no histological evidence of disease.19883369054
[effect of the composition of the nutrient media and culture conditions on the growth of strains of campylobacter jejuni and c. coli].the influence of the composition of culture media and cultivation conditions on the growth of campylobacter strains has been studied. blood-containing media have been found to ensure the most favorable growth conditions. no differences between the use of rct supplement (rifampicin, cephalothin and trimethoprim in amounts of 10, 15 and 5 mg/l respectively) and dc supplement (0.1% of sodium deoxycholate and 15 mg/l of cephalothin), as well as between different methods used for the creation of micr ...19883364070
detection of campylobacter jejuni with a synthetic oligonucleotide probe. 19883361728
[campylobacter jejuni: sepsis and meningitis in an adult without risk factors].a case of campylobacter jejuni meningitis with associated septicemia and positive stool culture in a normal host is presented. clinical findings, course of the illness and serologic data are compared with data from the literature. the possibility of infection due to a serum-resistant strain of campylobacter jejuni is raised. retrospective analysis of immunity revealed normal humoral and cellular immunity in a healthy patient one year later.19883358105
campylobacter jejuni antibodies in nigerian children.titers of complement-fixing (cf) antibody to campylobacter jejuni were demonstrated in 87 (36.7%) of 237 infants 6 to 15 months old in jos, nigeria. of the total number of children examined, 81 had acute diarrhea and 27 of them (33.3%) were found to have cf antibodies in their serum. the remaining 156 children were asymptomatic, and 60 (38.4%) of them had cf antibodies. in the diarrheal group, 27 of 75 children 6 to 8 months old were cf antibody positive. there was no significant difference in t ...19883356797
ultrastructure of campylobacter jejuni in gamma-irradiated mouse jejunum.this paper describes the ultrastructure of intracellular elongated, transitional and coccoid forms of campylobacter jejuni, in irradiated mouse jejunum infected both in vitro and in vivo and in cultured human skin fibroblasts. jejunum of irradiated mouse incubated for 1 hour under conditions favorable to the organisms showed minimal tissue degeneration. the intracellular organisms in this material were free cytoplasmic forms showing inner membrane degeneration, loss of cytoplasmic granules, and ...19883354638
a-61827 (a-60969), a new fluoronaphthyridine with activity against both aerobic and anaerobic bacteria.a-61827 (a-60969 is the hydrochloric salt of a-61827) is a new aryl-fluoronaphthyridine which is active against aerobic and anaerobic bacteria. the mics of a-61827 for 90% of strains (mic90) of staphylococci and streptococci were less than or equal to 1 microgram/ml and were generally 1 to 4 twofold dilutions less than those of ciprofloxacin for these bacteria. the mic90s of a-61827 for members of the family enterobacteriaceae and pseudomonas aeruginosa were also less than or equal to 1 microgra ...19883348609
persistent campylobacter jejuni infections in patients infected with the human immunodeficiency virus (hiv).we identified campylobacter jejuni infections in four patients infected with the human immunodeficiency virus (hiv); three had persistent and severe c. jejuni infections. multiple isolates obtained from each patient had the same biochemical and serotypic characteristics, indicating recurrent infection rather than reinfection with unrelated strains. serum antibody responses to c. jejuni group antigens by enzyme-linked immunosorbent assay were markedly impaired in the three patients with persisten ...19883348562
experimental campylobacter jejuni infection in humans.two strains of campylobacter jejuni ingested by 111 adult volunteers, in doses ranging from 8 x 10(2) to 2 x 10(9) organisms, caused diarrheal illnesses. rates of infection increased with dose, but development of illness did not show a clear dose relation. resulting illnesses with strain a3249 ranged from a few loose stools to dysentery, with an average of five diarrheal stools and a volume of 509 ml. infection with strain 81-176 was more likely to cause illness, and these illnesses were more se ...19883343522
evaluation of the campyslide agglutination test for confirmatory identification of selected campylobacter species.the utility of a rapid latex slide agglutination test (campyslide; bbl microbiology systems, cockeysville, md.) in detecting selected campylobacter spp. was evaluated and compared with that of conventional identification methods. isolated colonies suggestive of campylobacter spp. were tested directly from primary selective media after incubation at 42 degrees c under microaerophilic conditions. stock cultures of campylobacter jejuni (n = 27) and c. coli (n = 3) were correctly confirmed to the ge ...19883343315
characterization of an unclassified microaerophilic bacterium associated with gastroenteritis.four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ...19883343302
campylobacter jejuni infection with acute self limiting colitis and polyarthritis. 19883338828
experimental colonization of broiler chicks with campylobacter jejuni.minimal colonization inocula for two broiler strains of campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. individually housed chicks were exposed to a single oral or cloacal challenge. diarrhoeal symptoms were absent in all 380 chicks included in the study. chick susceptibility to the two c. jejuni strains varied. colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. coloni ...19883338504
the extent of surface contamination of retailed chickens with campylobacter jejuni serogroups.eighty-two chickens purchased at 11 retailers (supplied by 12 wholesalers) in the south of england were cultured for campylobacter jejuni by a method involving total immersion. the organism was isolated from 22 (48%) of 46 fresh birds, 12 of 12 uneviscerated (new york dressed) birds, but only 1 of 24 frozen birds. viable counts of up to 1.5 x 10(6)/chicken were obtained from fresh birds and 2.4 x 10(7)/chicken from uneviscerated birds. surface swabbing of breasts, thighs, wings and vents of fres ...19883338503
mucosal and systemic immunity to campylobacter jejuni in rabbits after gastric inoculation.the mucosal and systemic immune responses to campylobacter jejuni were studied in rabbits receiving gastric inoculation with live organisms. a lavage procedure was used to facilitate repeated monitoring of the intestinal immune response to c. jejuni. immunity to c. jejuni was determined by secondary challenge by using the removable intestinal tie adult rabbit diarrhea (ritard) model and monitoring for resistance to colonization and bacteremia. oral-gastric inoculation of normal rabbits produced ...19883335413
isolating campylobacter jejuni in an atmosphere made oxygen deficient using pyrogallol in a candle jar. 19873330104
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
paper-disc method for campylobacter hippurate-hydrolysis test. a note.paper discs were impregnated with sodium hippurate dissolved in 1/15 phosphate buffer ph 7.2. the discs, each containing 2 mg substrate, were stored in dry state at room temperature. after adding a disc to bacteria suspended in distilled water, and incubated at 37 degrees c for 2 h, one drop of skirrow and benjamin's ninhydrin reagent was added and the reaction was read after re-incubation at 37 degrees c for 10 min. 169 campylobacter strains were tested. the results for a total of 169 strains w ...19873326391
[reactive arthritis in enteral infections].certain gastrointestinal tract infections can trigger reactive arthritis. the best known triggering agents are yersinia, salmonella, shigella, campylobacter jejuni, and possibly clostridium difficile. the clinical findings show a subacute onset of mono- or oligoarthritis, less often of asymmetric polyarthritis with predominance of the lower limbs. the clinical picture is somewhat modified by the genetic constitution (hla type) of the patient. hla-b27-positive patients appear to have more severe ...19873326361
epidemiological studies on salmonella and campylobacter jejuni.an overview is given of investigations concerning the epidemiology of salmonella, carried out at the national institute of public health and environmental hygiene in bilthoven, the netherlands, during the last thirty years. it is made clear that salmonella, because of its ubiquitous occurrence and its large variety in sero- and phage-types, is the organism of choice to study the epidemiological pathways of pathogens between man, animals and the environment. it is demonstrated that these are in f ...19873321686
human yersinia enterocolitica infection in the eastern cape. a report on 18 cases and a review of yersiniosis in the rsa.yersinia enterocolitica infections with a wide spectrum of clinical presentations have been reported in south africa. an additional 18 cases were encountered in the eastern cape region during the period may 1982 - december 1984. y. enterocolitica isolates comprised 1% of 1,634 faecal examinations performed by the microbiology department of the south african institute for medical research in port elizabeth during this period, compared with isolation rates of 5.1% for shigellae, 3.7% for salmonell ...19873321485
frequency of escherichia coli o157:h7 isolation from stool specimens.during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ...19873319115
in vitro activity of ciprofloxacin compared to trimethoprim-sulfamethoxazole against campylobacter spp., shigella spp. and enterotoxigenic escherichia coli causing travellers' diarrhea in egypt.the in vitro activity of ciprofloxacin against bacterial enteropathogens isolated from cases of travellers' diarrhea in egypt was compared to trimethoprim (tmp) and trimethoprim-sulfamethoxazole (sxt). no resistance to ciprofloxacin was noted for any of the campylobacter jejuni/coli, shigella spp., and enterotoxigenic escherichia coli strains examined. however, resistance to tmp and sxt was noted among these same strains. because of its broad spectrum and lack of resistance, ciprofloxacin is pot ...19873313681
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
small rodents and other mammals associated with mountain meadows as reservoirs of giardia spp. and campylobacter spp.sixty-five percent (469 of 722) of the fecal samples collected from small rodents in the central washington cascade mountains were positive for giardia spp. trapping studies showed that microtines of the genus microtus were heavily infected with the parasite. morphologically the cysts and trophozoites were of the giardia duodenalis type. small-rodent populations appear to maintain their infection throughout the year. our data suggest that there is no difference in the percentage of positive anim ...19873310881
clinical aspects of infection with yersinia enterocolitica in adults.yersinia enterocolitica was isolated from the feces of 29 patients over a three-year period following the introduction of a selective culture medium. y. enterocolitica was the third most common enteric pathogen after campylobacter jejuni and salmonella in this series of 3795 specimens from a predominantly adult population. the isolation rate of y. enterocolitica was 0.9% and this represented 15.8% of positive cultures. the usual symptoms of yersinia infection were diarrhea (93%) and abdominal pa ...19873304248
the aetiology of diarrhoea in children at an urban hospital in bangladesh.enteric pathogens were studied in 104 cases with acute diarrhoea and in 74 age and sex matched concurrent controls. one or more pathogens were isolated from 59.1% of cases compared with 20.4% of controls (p less than 0.001). single enteropathogens were detected in 33.7% and multiple enteropathogens in 25.4% of the cases. enteropathogenic escherichia coli (epec), campylobacter jejuni, rotavirus, vibrio cholerae non-o1 and enterotoxigenic escherichia coli were the major pathogens detected. the hig ...19873303485
structural and antigenic properties of lipopolysaccharides from serotype reference strains of campylobacter jejuni.to investigate the molecular basis for heat-stable antigenic diversity in campylobacter jejuni, lipopolysaccharides (lpss) from serotype reference strains and serotyped isolates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining and immunoblotting. by silver staining, only low-mr components, consisting of one major band and as many as three minor bands ranging in mr from 4,500 to 5,000, were detected. however, by immunoblotting with homologous ...19873301678
two-dimensional gel electrophoresis and immunoblotting of campylobacter outer membrane proteins.we characterized outer membrane proteins (omps) from selected campylobacter jejuni, c. coli, and c. fetus strains by two-dimensional gel electrophoresis (2dge), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), and by immunoblotting with immune rabbit serum. the flagellar band with a molecular mass of 63 kilodaltons (kda) demonstrated previously by one-dimensional sds-page was shown by 2dge to consist of one or several charge trains, depending u ...19873298060
acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ...19873296648
campylobacter jejuni/coli. 19883285863
[detection of campylobacter jejuni and c. coli in waste water].for the detection of c. jejuni and c. coli in waste water the content of rifampicin in selective media was reduced from 10 micrograms/ml (bolton and robertson 1982) to 5 micrograms/ml and the content of polymymyxin b was increased from 0,25 microliter/ml (skirrow 1977) to 2.5 micrograms/ml. on the basis of this modification the isolation of c. jejuni and c. coli from waste water samples by membrane filter technique as well as by enrichment procedure was possible. before inoculation of waste wate ...19883285625
a prospective study of serum antibody responses to enterotoxinogenic escherichia coli in swedish travellers.serological responses to relevant enterotoxinogenic escherichia coli (etec) antigens were studied in 85 swedish travellers to subtropical and tropical areas. serum samples were collected from the travellers before and then after 3-5 weeks, i.e. within a week after return to sweden, when a faecal specimen was also taken. 40% of the participants had traveller's diarrhoea during their visit abroad, while 21% reported "loose stools" and 39% had no such problems. etec was rarely isolated from the sto ...19883283923
[in vitro bacteriostatic and bactericidal effect of ciprofloxacin and others quinolone derivatives on campylobacter jejuni].the in vitro bacteriostatic (mic) and bactericidal (mbc) activities of ciprofloxacin and seven other quinolone derivatives on campylobacter jejuni from human origin were determined. ciprofloxacin, pefloxacin and rosoxacin exhibited the best bacteriostatic and bactericidal activities. for the three compounds the mic90 was less than or equal to 0.33 microgram/ml while the mbc90 was resp. 0.36, 0.56 and 0.56 microgram/ml. the mbc values were always significantly higher than the mic values (p less t ...19883279386
guillain-barré syndrome following campylobacter jejuni enteritis. report of three cases and review of the literature.we describe three cases of guillain-barré syndrome (gbs) associated with campylobacter jejuni enteritis and review the data from eight other cases described in detail in the literature. the recent recognition of this association is probably due to improved stool culture technique. in comparison with gbs associated with respiratory infections, the delay between the first symptoms of infection and the development of gbs is somewhat longer, an observation in accordance with gbs associated with gast ...19883277576
campylobacter ileocolitis mimicking acute appendicitis: differentiation with graded-compression us.in nine patients with clinical symptoms of acute appendicitis, graded-compression ultrasound (us) did not depict the appendix but instead demonstrated mural thickening of the terminal ileum and part of the colon, as well as moderately enlarged mesenteric lymph nodes. in one patient a normal appendix was removed; eight patients recovered without treatment. infection with campylobacter jejuni was proved in all nine patients. in four patients, an unnecessary appendectomy was avoided because of the ...19883277242
high-voltage electroporation of bacteria: genetic transformation of campylobacter jejuni with plasmid dna.electroporation permits the uptake of dna by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. we investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen campylobacter jejuni as a model system. this report demonstrates that the application of high-voltage discharges to bacterial cells permits genetic transformation. our method involves exposure of a ...19883277182
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