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what do herbalists suggest to diabetic patients in order to improve glycemic control? evaluation of scientific evidence and potential risks.in the course of 12 continuing education seminars given in different regions of italy in 2001, we distributed a questionnaire to all the attending herbalists asking information about the herbal remedy and dietary supplement they mainly recommended to subjects who required a "natural" treatment to control glycemia. we distributed 720 questionnaires and we received 685 completed ones. we have compiled a short review on the efficacy and safety of the 10 most frequently advised products for each cat ...200415666575
color, betalain pattern, and antioxidant properties of cactus pear (opuntia spp.) clones.total phenolics, ascorbic acid, and betalain contents of differently colored cactus pear clones (nine opuntia ficus-indica [l.] mill. clones and one o. robusta wendl. clone) were investigated and related to their respective antioxidant potential assessed by trolox-equivalent antioxidant capacity (teac) and oxygen radical absorbance capacity (orac) assays. teac and orac values were very highly correlated with each other and also with values for total phenolics, betalain contents, and ascorbic aci ...200515656686
partitioning of (13)c-photosynthate from spur leaves during fruit growth of three japanese pear (pyrus pyrifolia) cultivars differing in maturation date.in fruit crops, fruit size at harvest is an important aspect of quality. with japanese pears (pyrus pyrifolia), later maturing cultivars usually have larger fruits than earlier maturing cultivars. it is considered that the supply of photosynthate during fruit development is a critical determinant of size. to assess the interaction of assimilate supply and early/late maturity of cultivars and its effect on final fruit size, the pattern of carbon assimilate partitioning from spur leaves (source) t ...200515655106
alpha-gustducin immunoreactivity in the airways.the g-protein subunit alpha-gustducin is a marker of chemoreceptive cells. in the present study, we examined the immunohistochemical localization of alpha-gustducin in rat airway epithelium both by light and electron microscopy. alpha-gustducin immunoreactivity was found in solitary cells that presented ultrastructural features of chemoreceptor cells, i.e. flask-shaped or pear-shaped, with an apical process with thin microvilli protruding into the lumen. the immunostaining was mainly concentrate ...200415654652
gorham-stout disease--stabilization during bisphosphonate treatment.a 45-year-old woman presented with recent onset of left-sided chest pain. on clinical examination, these symptoms seemed to be strictly localized to a region that was marked by a long-standing cutaneous erythematous lesion. laboratory results showed no gross abnormalities. radiological imaging including conventional x-ray, mri scans, and 3d ct reconstruction of the rib cage revealed circumscript destruction of the left lateral ribs 9-11. histological analysis of a rib biopsy showed angiomatous h ...200515647829
[relationship between breaking of dormancy and reactive oxygen species metabolism in flower buds of pear].the metabolism of reactive oxygen species in pear (pyrus bretschneideri rehd.) flower buds changes greatly during their natural dormancy in winter. the o(-.)(2) production rate increases rapidly during the period of dormancy, but decreases when dormancy finishes (fig. 5). h(2)o(2) content goes up significantly at the early stage of dormancy, but afterwards falls gradually (fig. 5). however, ascorbic acid (asa) and reduced glutathione (gsh) contents show a different changing trend: descending at ...200415643086
[osmolality of frequently consumed beverages].the objective of this work was to determine the osmolality of beverages frequently consumed by children and adolescents due to the scarce information available in our country. the samples were grouped as follows: milks; refreshments; beverages based on fruits, vegetables, cereals, and tubers; sport drinks; energizing drinks; oral rehydrating solutions; reconstituted drinks and infusions. a vapor pressure digital osmometer was used, five samples of each beverage from different lots were analyzed. ...200415602899
the plant pathogen erwinia amylovora produces acyl-homoserine lactone signal molecules in vitro and in planta.we report for the first time the production of acyl homoserine lactones (ahls) by erwina amylovora, an important quarantine bacterial pathogen that causes fire blight in plants. e. amylovora produces one n-acyl homoserine lactone [a n-(3-oxo-hexanoyl)-homoserine lactone or a n-(3-hydroxy-hexanoyl)-homoserine lactone] quorum sensing signal molecule both in vitro and in planta (pear plant). given the involvement of ahls in plant pathogenesis, we speculate that ahl-dependent quorum sensing could pl ...200415598530
occurrence and significance of cryptococcus neoformans in vegetables and fruits.cryptococcus neoformans was isolated from three of 437 (0.6%) samples of a wide variety of vegetables collected from a number of markets in delhi. the vegetables yielding c. neoformans were tomato, lycopersicon esculentum (1/70), 'vegetable sponge', luffa cylindrica (1/56) and brinjal, solanum melongena (1/55). only solitary samples of these three vegetables were found to be positive. in vitro studies at 25 degrees c revealed that a reference c. neoformans isolate readily colonized, as did candi ...200015597680
evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates.the usefulness of various suggested species demarcation criteria was compared in attempts to determine the taxonomic status of ten new tombusvirus isolates. five of them (lim 1, 2, 3, 5 and 6) were obtained from different sources of commercially grown statice (limonium sinuatum), two (gyp 1 and 2) from different sources of commercially grown gypsophila paniculata and three from water samples, i.e. from a small river (schunter) in northern germany, from a brook (near dossenheim) in southern germa ...200415593416
development of an enzyme-linked immunosorbent assay for the detection of the fungicide fenarimol.to develop an enzyme-linked immunosorbent assay for the fungicide fenarimol, two synthesized haptens, haptens-1 and -2, and the purchased 4,4'-dda were conjugated to carrier proteins (bsa, klh, and ova). polyclonal antibodies raised against hapten-1,2-klh conjugates in rabbits and the coating antigens of hapten-1,2-bsa conjugates, hapten-2-ova conjugate, and 4,4'-dda-bsa conjugate were screened and selected for the homologous and/or heterologous elisa formats. two competitive indirect elisas wer ...200415563196
correction of facial deformity using a red iii device in a patient with antley-bixler syndrome.antley-bixler syndrome was first described in 1975 and to date at least 44 cases have been reported. in addition to brachycephaly, this syndrome is associated with midface hypoplasia, bilateral radiohumeral synostosis, multiple joint contractures, long bone fractures, dysplastic ears, "pear shaped" nose, and occasionally urogenital or cardiac defects. the authors report a case in a 23-year-old man with severe midface deficiency treated using distraction osteogenesis. a modified le fort iii osteo ...200415547402
attitudes and beliefs among mexican americans about type 2 diabetes.hispanics in the united states have a disproportionately high risk for non-insulin-dependent diabetes mellitus (type 2 diabetes) compared with non-hispanic whites. little is known of the attitudes and beliefs about diabetes in this group. using data from six focus groups of 42 mexican americans (14 men and 28 women), we characterized perceptions about the causes of and treatments for type 2 diabetes. many participants believed diabetes is caused by having a family history of the disease, eating ...200415531816
nucleotide sequences of a korean isolate of apple stem grooving virus associated with black necrotic leaf spot disease on pear (pyrus pyrifolia).pear black necrotic leaf spot (pbnls) is a disease of pears caused by capillovirus-like particles, which can be observed under the electron microscope. the disease was analyzed by western blot analysis with antisera raised against apple stem grooving virus (asgv) coat protein. cdnas covering the entire genome were synthesized by rt-pcr and race using rna isolated from chenopodium quinoa infected with sap extracted from pear leaves carrying black necrotic spot disease. the complete genome sequenc ...200415528995
low temperature, but not photoperiod, controls growth cessation and dormancy induction and release in apple and pear.in contrast to most temperate woody species, apple and pear and some other woody species of the rosaceae family are insensitive to photoperiod, and no alternative environmental seasonal signal is known to control their dormancy. we studied growth and dormancy induction in micropropagated plants of four apple (malus pumila mill.) and one pear (pyrus communis l.) commercial rootstock cultivars in controlled environments. the results confirm that growth cessation and dormancy induction in apple and ...200515519992
the spinal trigeminal nucleus--considerations on the structure of the nucleus caudalis.the caudal part (nucleus caudalis) of the spinal trigeminal nucleus is considered to be the site of the second order neurons of the nociceptive pathways of the face. recent studies have supported the co-participation in these circuits of the oral part of the same nucleus (nucleus oralis). the aims of the present study are: 1) to determine the morphology of the nucleus caudalis in human preparates; 2) to consider whether there is any structural basis for the pathways of signal transmission observ ...200415478109
cytoarchitecture of the normal rat olfactory epithelium: light and scanning electron microscopic studies.the three-dimensional cytoarchitecture of the normal rat olfactory epithelium was examined by scanning electron microscopy (sem) of koh digested tissues as well as by light and transmission electron microscopy of plastic sections. observations specimens from the lateral side of the olfactory epithelium allowed identification of four cell types by their surface structure: olfactory neurons, supporting cells, basal cells, and duct cells of the bowman's gland. the olfactory neurons were characteriz ...200415468955
response of organic and inorganic carbon and nitrogen to long-term grazing of the shortgrass steppe.we investigated the influence of long-term (56 years) grazing on organic and inorganic carbon (c) and nitrogen (n) contents of the plant-soil system (to 90 cm depth) in shortgrass steppe of northeastern colorado. grazing treatments included continuous season-long (may-october) grazing by yearling heifers at heavy (60-75% utilization) and light (20-35% utilization) stocking rates, and nongrazed exclosures. the heavy stocking rate resulted in a plant community that was dominated (75% of biomass pr ...200415453402
effects of culture media and environmental factors on mycelial growth and pycnidial production of potebniamyces pyri.potebniamyces pyri (anamorph phacidiopycnis piri) is the causal agent of phacidiopycnis rot of apples and pears. the disease has recently been recognized in pears in the usa. little information on the basic biology of the fungus is available. in this paper, we report the effects of culture media, temperature, water potential and ph on mycelial growth, and the effects of media and light on pycnidia production. prune juice agar was the best for rapid mycelial growth. pear juice agar, apple juice a ...200415449598
irradiation disinfestation of apple maggot (diptera: tephritidae) in hypoxic and low-temperature storage.apple maggot, rhagoletis pomonella (walsh), is a quarantine pest of apples, malus domestica borkhausen, and pears, pyrus communis l., shipped from much of the united states and canada. as such, these fruits shipped from infested areas to uninfested areas must undergo a quarantine disinfestation treatment. the objective of this research was to develop irradiation quarantine treatments against apple maggot considering that fruit hosts may be stored under hypoxic or cold conditions when they are ir ...200415384333
identification of caveolae and their signature proteins caveolin 1 and 2 in the lens.this study shows that caveolae are present in lens epithelia of rabbit and guinea pig under normal conditions. caveolae are unique lipid membrane microdomains observed in many cell types. they are believed to play crucial roles in a variety of basic physiological functions including signal transduction, lipid and transcellular transport. using tem, immunocytochemistry and immunoblotting we show for the first time the existence of caveolae and the co-localization of their signature marker integra ...200415381033
expression of viral eps-depolymerase reduces fire blight susceptibility in transgenic pear.erwinia amylovora is the causal agent of fire blight of maloideae. one of the main pathogenicity factors of this bacterium is the exopolysaccharide (eps) of its capsule. in this paper, we used genetic transformation tools to constitutively express an eps-depolymerase transgene in the pear (pyrus communis l.) cv. passe crassane with the aim of decreasing its high susceptibility to fire blight. expression of the depolymerase gene in 15 independent transgenic clones led, on average, to low depolyme ...200515375629
determination of key odorant compounds in freshly distilled cognac using gc-o, gc-ms, and sensory evaluation.this aim of this work was to identify the odorant compounds responsible for the typical sensory descriptors attributed to freshly distilled cognac spirits, not matured in barrels. panelists were first selected and trained for gas chromatography-olfactometry. among the 150 volatile compounds identified by gas chromatography-mass spectrometry analysis, only 34 are mainly responsible for the odors detected in the spirits. the "butter" descriptor is explained by the presence of diacetyl, the "hay" d ...200415373408
[gorham-stout disease: report of a case affecting the right hand with a follow-up of 24 years].gorham-stout disease is a rare idiopathic syndrome with distinctive clinical, pathologic and radiologic features. it is a variant form of osseus angiomatosis associated with massive osteolysis of bone. usually appearing after trauma, the disease is described to occur at any age. especially in case of thoracic involvement (chylothorax), lethal outcomes are reported. in medline, about 200 cases have been described. a patient with osteolysis of the right hand following contusion at the age of two y ...200415368153
expression of ripening-related genes in prickly pear (opuntia sp.) fruits.to throw light on the expression of ripening-related genes in prickly pear (opuntia sp.) fruits and on the possible role of the gaseous hormone ethylene in nonclimacteric fruit ripening, cdna fragments that showed high homologies with 1-aminocyclopropane-1-carboxylate (acc) synthase and acc oxidase cdnas from other plants were cloned and partially characterized. thus, the corresponding genes were accordingly named opaccs-1 and opacco-1, after opuntia acc synthase-1 and opuntia acc oxidase-1, res ...200315354791
development of a one tube-one step rt-pcr protocol for the detection of seven viroids in four genera: apscaviroid, hostuviroid, pelamoviroid and pospiviroid.a one tube-one step rt-pcr was developed for the detection of seven viroids (apple scar skin viroid, apple dimple fruit viroid, pear blister canker viroid, hop stunt viroid, chrysanthemum stunt viroid, citrus exocortis viroid and peach latent mosaic viroid) in four genera that infect eight plant species. the efficiency and specificity of this method were optimized by the use of moloney-murine leukemia virus (m-mlv) reverse-transcriptase and hotstartaq dna polymerase which allowed increase sensit ...200415350729
characterisation and transferability of apple ssrs to two european pear f1 populations.european pear (pyrus communis l.) is among the important fruit species for which only few genetic studies have been carried out. available evidence indicates that simple sequence repeats (ssr) are very useful as molecular markers because they are codominant, highly polymorphic, abundant and reproducible. the present paper reports more than 100 apple ssr markers in two populations of european pear; a total of 41 ssr markers were then positioned on a genetic linkage map of the cross 'passe crassan ...200415340685
european, chinese and japanese pear fruits exhibit differential softening characteristics during ripening.softening characteristics were investigated in three types of pear fruit, namely, european pear 'la france', chinese pear 'yali', and japanese pear 'nijisseiki'. 'la france' fruit softened dramatically and developed a melting texture during ripening, while 'yali' fruit with and without propylene treatment showed no change in flesh firmness and texture during ripening. non-treated 'nijisseiki' did not show a detectable decrease in flesh firmness, whereas continuous propylene treatment caused a gr ...200415333646
gorham-stout disease of costo-vertebral localization: radiographic, scintigraphic, computed tomography, and magnetic resonance imaging findings.gorham-stout disease is a clinical, radiographic, and histological entity characterized by progressive osteolysis as a result of a histologically benign vascular proliferation involving bone. we present a case involving the cervical and thoracic spine and the osseous thorax, with attention to the clinical, radiographic, scintigraphic, computed tomography, and magnetic resonance imaging findings. these subjects are discussed in the light of the literature.200415323402
post-translational modifications of arabinogalactan-peptides of arabidopsis thaliana. endoplasmic reticulum and glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation of proline.we have developed a method for separating the deglycosylated protein/peptide backbones of the small arabinogalactan (ag)-peptides from the larger classical arabinogalactan-proteins (agps). agps are an important class of plant proteoglycans implicated in plant growth and development. separation of ag-peptides enabled us to identify eight of 12 ag-peptides from arabidopsis thaliana predicted from genomic sequences. of the remaining four, two have low abundance based on expressed sequence tag datab ...200415322080
algr functions in algc expression and virulence in pseudomonas syringae pv. syringae.pseudomonas syringae pv. syringae strain ff5 is a phytopathogen associated with a rapid dieback on ornamental pear trees. p. syringae and the human pathogen pseudomonas aeruginosa produce the exopolysaccharide alginate, a copolymer of mannuronic and guluronic acid. in p. aeruginosa, the response regulator algr (algr1) is required for transcription of algc and algd, which encode key enzymes in the alginate biosynthetic pathway. in p. syringae ff5, however, algr is not required for the activation ...200415289569
'candidatus phytoplasma', a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects.the trivial name 'phytoplasma' has been adopted to collectively name wall-less, non-helical prokaryotes that colonize plant phloem and insects, which were formerly known as mycoplasma-like organisms. although phytoplasmas have not yet been cultivated in vitro, phylogenetic analyses based on various conserved genes have shown that they represent a distinct, monophyletic clade within the class mollicutes. it is proposed here to accommodate phytoplasmas within the novel genus 'candidatus (ca.) phyt ...200415280299
'candidatus phytoplasma mali', 'candidatus phytoplasma pyri' and 'candidatus phytoplasma prunorum', the causal agents of apple proliferation, pear decline and european stone fruit yellows, respectively.apple proliferation (ap), pear decline (pd) and european stone fruit yellows (esfy) are among the most economically important plant diseases that are caused by phytoplasmas. phylogenetic analyses revealed that the 16s rdna sequences of strains of each of these pathogens were identical or nearly identical. differences between the three phytoplasmas ranged from 1.0 to 1.5% of nucleotide positions and were thus below the recommended threshold of 2.5% for assigning species rank to phytoplasmas under ...200415280295
seasonal susceptibility of 'bartlett' pears to codling moth (lepidoptera: tortricidae) infestation and notes on diapause induction.seasonal susceptibility of 'bartlett' pear, pyrus communis l., to codling moth, cydia pomonella (l.), infestation, successful completion of larval development after infestation, and the induction of c. pomonella diapause was studied from 1992 through 1995. the seasonal variation in c. pomonella infestation and larval survival were effected by changes in fruit maturity. in late may through mid-june, pears were hard and were not as successfully infested by c. pomonella and produced less larvae com ...200415279281
fatty acid content of selected seed oils.fatty acid content of selected seed oils from world-wide edible fruits, ceratonia ciliqua (carob) from caesalpiniaceae family, diospyros kaki (persimmon) from ebenaceae family, zizyphus jujuba (jujube) from rhamnaceae family, and persea gratissima (avocado pear) from lauraceae family, were determined by capillary gas chromatography- mass spectrometry (gc-ms) to find new natural sources for essential fatty acids. among the seed oils analyzed, ceratonia ciliqua has been found to have the highest e ...200215277087
asian pear pectin administration during presensitization inhibits allergic response to ovalbumin in balb/c mice.a type of respiratory disorder resembling some aspects of human allergic asthma can be induced in mice using ovalbumin. the factors that influence the etiology of asthma are poorly understood even though cytokines are known to play a pivotal role. the purpose of this study was to test the hypothesis whether an administration of asian pear pectin during presensitization could suppress allergic response to ovalbumin in balb/c mice.200415253858
close-wedge osteotomy for bony locking stiffness of the elbow in gorham disease patients: a case report.gorham disease is a so-called massive idiopathic osteolysis or vanishing bone disorder. massive osteolysis remains an enigmatic condition that involves various skeletal locations and is caused by endothelial proliferation. the diagnosis is difficult and is established via the association of clinical, radiologic and histologic pictures. treatment modalities yield variable results. we report a case of vanishing bone in the elbow joint and carpal bones following trauma. this 13-year-old boy complai ...200415233238
modulation of intracellular calcium concentrations and t cell activation by prickly pear polyphenols.opuntia ficus indica (prickly pear) polyphenolic compounds (ofpc) triggered an increase in [ca2+]i in human jurkat t-cell lines. furthermore, ofpc-induced rise in [ca2+]i was significantly curtailed in calcium-free buffer (0% ca2+) as compared to that in 100% ca2+ medium. preincubation of cells with tyrphostin a9, an inhibitor of ca2+ release-activated ca2+ (crac) channels, significantly diminished the ofpc-induced sustained response on the increases in [ca2+]i. lanthanum and nifedipine, the res ...200415228091
intra-oral acid production associated with eating whole or pulped raw fruits.the hypotheses that raw fruits, whether whole or pulped, were cleared rapidly from the mouth and that the sugars in the whole and pulped fruits are fermented with equal efficiency to acids by the oral microflora were tested in this study. groups of 7-9 adult subjects chewed 10 g of raw, whole or pulped fruit (apple, banana, orange, pear and pineapple) for 1 min and whole, unstimulated saliva samples were collected during the following 60-min interval. each saliva sample was assayed for the conce ...201615181333
fluorescence shell: a novel view of sclereid morphology with the confocal laser scanning microscope.confocal laser scanning microscopy (clsm) was used to observe sclereids from stems of avicennia germinans and from fruits of two species of pear (pyrus calleryana "bradford" and p. communis "red bartlett"). the images obtained from thick (25 to 100 microm) free-hand sections were, in certain respects, far superior to those obtained by other, more invasive and time-consuming microscopic techniques upon which previous reports of sclereid morphology were based. the cell wall surfaces, including the ...200415170758
sodium bicarbonate enhances biocontrol efficacy of yeasts on fungal spoilage of pears.the addition of 2% (w/v) sodium bicarbonate (sbc) in the suspensions of antagonistic yeast cryptococcus laurentii or trichosporon pullulans significantly limited spore germination and germ tube elongation of penicillium expansum and alternaria alternata in potato dextrose broth (pdb) medium. biocontrol activity of c. laurentii or t. pullulans against postharvest decay caused by p. expansum and a. alternata in pear fruits was significantly increased when c. laurentii or t. pullulans combined with ...200415163586
effect of the epicuticular waxes of fruits and vegetables on the photodegradation of rotenone.the effect of epicuticular waxes extracted from fruits (apple, nectarine, pear, and plum) and vegetables (tomato and eggplant) on the photodegradation of rotenone was studied. the waxes affected the decay rate and the degradation pathway of this botanical insecticide. tomato, nectarine, and plum waxes decreased the photodegradation rate compared to controls, whereas apple and pear waxes increased it. rotenone irradiated under sunlight without waxes gave seven photoproducts; in contrast, in the p ...200415161214
self-reported food hypersensitivity in sweden, denmark, estonia, lithuania, and russia.the main aim of the study was to describe the differences between some northern countries regarding what foods, according to the patients, elicit hypersensitivity symptoms.200415160445
shape changes in caprine erythrocytes exposed to chlorpromazine and lysolecithin.stomatocytic and echinocytic transformations of caprine erythrocytes were studied in vitro using chlorpromazine as a stomatocyyic agent and lysolecithin as an echinocytic agent. morphologic changes in erythrocytes generally varied with the cell shape and the concentration of the substances used. discoytic, triangular, and pear-shaped red cells common to normal goats, exhibited classical stomatocytic and echinocytic changes with formation of spherostomatocytes, sphero-echinocytes, and spherocytes ...198915156515
ethical issues in the neonatal intensive care unit.the case of baby y presented a difficult and complex ethical dilemma for the family and the staff involved. the issues of religious beliefs and law, up-holding these beliefs in the center of a religious community, financial concerns, and health care workers disagreeing about carrying out treatments made this case one that few will forget. when asked after baby y died how they felt, many members of the staff answered that it should not have gone on as long as it did and that they learned a lot fr ...200415145372
[effects of zinc and boron nutrition on balsam pear (momordica charantia) yield and quality, and polyamines, hormone, and senescence of its leaves].field trial and physiological determination on the effects of zinc and boron nutrition on balsam pear (momordica charantia) yield and quality and on polyamines, hormone, and senescence of its leaves showed that application of znso4.7h2o and na2b4o7.10h2o on zn and b deficient soils could increase balsam pear yield and its contents of protein, vc and 17 sorts of amino acids, especially the contents of essential amino acids for human body, but decrease the no3(-)-n content. the reason was that zn ...200415139192
polygalacturonase and ethanol production in kluyveromyces marxianus: potential use of polygalacturonase in foodstuffs.the coproduction of ethanol and polygalacturonase (pg) in a pilot-scale batch fermentor using yeast extract--glucose (yd)--and sugar beet molasses (sbm)-based media was implemented utilizing a new high-pg-producing strain of kluyveromyces marxianus. a certain growth inhibition was observed in sbm medium, causing ethanol and pg production to be lower. ethanol productivity and accumulation values of 1.94 g/(l x h) and 40 g/l, respectively, were attained in yd, whereas the best fermentation efficie ...200415126703
dissection of the host range of the fungal plant pathogen alternaria alternata by modification of secondary metabolism.the filamentous fungus alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. the strawberry pathotype produces host-specific af-toxin and causes alternaria black spot of strawberry. this pathotype is also pathogenic to japanese pear cultivars susceptible to the japanese pear pathotype that produces ak-toxin. the strawberry pathotype produces two related molecular species, af-toxins i and ii: toxi ...200415066029
molecular differentiation of erwinia amylovora strains from north america and of two asian pear pathogens by analyses of pfge patterns and hrpn genes.in order to determine a possible genomic divergence of erwinia amylovora'fruit tree' and raspberry strains from north america, several isolates were differentiated by pulsed-field gel electrophoresis (pfge) analysis, the size of short dna sequence repeats (ssrs) and the nucleotide and deduced amino acid sequences of their hrpn genes. by pfge analysis european strains are highly related, whereas strains from north america were diverse and were further distinguished by the ssr numbers from plasmid ...200415049921
disappearing calvarium in gorham disease: mr imaging characteristics with pathologic correlation.gorham disease is a rare condition characterized by intraosseous neoplastic proliferation of hemangiomatous tissue with progressive, massive osteolysis. we present a pathologically proved case of gorham disease that involved the left parietal bone in a 23-year-old man. imaging studies including conventional radiography of the skull, ct, mr imaging, and technetium-99 m (tc-99 m) scintigraphy demonstrated a large skull defect without associated soft tissue mass over the left parietal skull. contra ...200415037464
management of fire blight: a case study in microbial ecology.suppression of the blossom-blight phase of fire blight is a key point in the management of this destructive and increasingly important disease of apple and pear. for blossom infection to occur, the causal bacterium, erwinia amylovora, needs to increase its population size through an epiphytic phase that occurs on stigmatic surfaces. knowledge of the ecology of the pathogen on stigmas has been key to the development of predictive models for infection and optimal timing of antibiotic sprays. other ...199815012499
free amino acid composition of quince (cydonia oblonga miller) fruit (pulp and peel) and jam.twenty-one free amino acids present in several samples of quince fruit (pulp and peel) and quince jam (homemade and industrially manufactured) were analyzed by gc/fid. the analyses showed some differences between quince pulps and peels. generally, the highest content in total free amino acids and in glycine was found in peels. as a general rule, the three major free amino acids detected in pulps were aspartic acid, asparagine, and hydroxyproline. for quince peels, usually, the three most abundan ...200414995121
the effect of different atmospheric ozone partial pressures on photosynthesis and growth of nine fruit and nut tree species.nursery stock of peach (prunus persica l. batsch, cv. o'henry), nectarine (p. persica l. batsch, cv. fantasia), plum (p. salicina lindel., cv. casselman), apricot (p. armeniaca l., cv. tilton), almond (p. dulcis mill., cv. nonpareil), prune (p. domestica l., cv. improved french), cherry (p. avium l., cv. bing), oriental pear (pyrus pyrifolia rehd., cv. 20th century), and apple (malus pumula mill., cv. granny smith) were planted in open-top chambers on april 1, 1988 at the university of californi ...199114972900
massive osteolysis (gorham disease) of the maxillofacial skeleton: report of 2 cases. 200414762756
some coccidial parasites of the lizard amphisbaena alba (reptilia: amphisbaenia: amphisbaenidae).five parasites are described in the lizard amphisbaena alba (amphisbaenidae) from the state of par , north brazil. mature oocysts of choleoeimeria amphisbaenae n. sp., are passed already mature in the faeces. they are ellipsoidal-cylindrical, average 33.7 x 22.8 m and are devoid of micropyle, oocyst residuum or polar body. the colourless wall is smooth and of 2 layers. the 4 dizoic sporocysts have no stieda body and average 13 x 9.3 m. endogenous stages develop in the epithelial cells of the g ...200414762520
effects of water stress cycles on turgor maintenance processes in pear leaves (pyrus communis).effects of water deficits on leaf turgor maintenance processes were analyzed for pear trees (pyrus communis l. cv. "barlett") grown in 120-liter containers. four irrigation treatments were applied: a well-watered control treatment, a spring water stress cycle (sp), a summer water stress cycle (su), and a spring plus summer water stress cycle (sp + su). for the sp treatment, water application was progressively reduced from 100 to 20% of the control dose over a period of 27 days in spring. for the ...199714759856
molecular markers derived from rapd, scar, and the conserved 18s rdna sequences for classification and identification in pyrus pyrifolia and p. communis.we generated rapd, scar, and conserved 18s rdna markers for classifying and identifying cultivars of pyrus pyrifolia (japanese pear) and p. communis (european pear). pcr amplification with selected specific primers-lch327up and lch327down-was performed using dna extracted from 25 p. pyrifolia and p. communis cultivars. the 1,380-bp fragment was amplified from p. communis cvs. beurre giffard, cascade, conference, clapp's favorite, packhams triumph, and winter nelis. rapd has only a dominant singl ...200414749847
genetic characterization of erwinia amylovora strains by amplified fragment length polymorphism.erwinia amylovora is one of the most important pathogens of pear and apple and is subject to strict quarantine regulations worldwide, although its patterns of dispersal are largely unknown. previous attempts to fingerprint e. amylovora strains by molecular techniques have detected very little polymorphism because of the high genetic homogeneity of this bacterium. our aim was to establish and test a typing method to quantify genetic diversity among strains of this plant pathogen.200414723691
isolation and characterisation of resistant-to-fermentation carbohydrate polymers from cultures of rhizopus nigricans grown on agrofood waste materials.rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. in all cultures, a carbohydrate polymer fraction remained resistant to fermentation. these fractions were isolated in gram amounts and characterised. the molecular weight distribution of the fractions and its sugar composition resembles those of the hairy-regions of the pectins. in the fractions, four main carbohydrates were found: 4-7 mol% rha, 42-59 ...200314719820
the phytoalexin-inducible multidrug efflux pump acrab contributes to virulence in the fire blight pathogen, erwinia amylovora.the enterobacterium erwinia amylovora causes fire blight on members of the family rosaceae, with economic importance on apple and pear. during pathogenesis, the bacterium is exposed to a variety of plant-borne antimicrobial compounds. in plants of rosaceae, many constitutively synthesized isoflavonoids affecting microorganisms were identified. bacterial multidrug efflux transporters which mediate resistance toward structurally unrelated compounds might confer tolerance to these phytoalexins. to ...200414714867
qualitative and quantitative chromatographic investigation of hydroquinone derivatives in pyrus communis l. flowers.the qualitative analysis and quantitative determination of hydroquinone derivatives (arbutin, pyroside) in the flowers of naturally growing pear tree and of its four cultivated varieties (pomaraƄczówka, lukasówka, klapsa, salisbury) were carried out. the comparative qualitative analysis of hydroquinone derivatives was investigated chromatographically (tlc). arbutin and pyroside were found in all the studied plant materials and free hydroquinone was found in buds of naturally growing pear. the co ...201314714860
a myosin inhibitor impairs auxin-induced cell division.the role of myosins for auxin-induced cell division was probed using the inhibitor 2,3-butanedione monoxime in the tobacco cell line vbi-0, where cell elongation and division are axially aligned under the control of auxin. a morphometric analysis revealed that cell division is blocked in a dose-dependent manner, whereas cell expansion continued. in addition, the polarity of terminal cells was impaired resulting in malformed, pear-shaped cells. early effects of the inhibitor are aberrant features ...200314714208
induction of antimicrobial 3-deoxyflavonoids in pome fruit trees controls fire blight.fire blight, a devastating bacterial disease in pome fruits, causes severe economic losses worldwide. hitherto, an effective control could only be achieved by using antibiotics, but this implies potential risks for human health, livestock and environment. a new approach allows transient inhibition of a step in the flavonoid pathway, thereby inducing the formation of a novel antimicrobial 3-deoxyflavonoid controlling fire blight in apple and pear leaves. this compound is closely related to natura ...200314713146
description of paeon asymboli n. sp. (copepoda: sphyriidae), parasitic on asymbolus spp. (catsharks) and a new host record for p. australis kabata, 1993.paeon asymboli n. sp. (copepoda: sphyriidae) is described and illustrated from two female specimens taken from the gills of a grey spotted catshark asymbolus analis (ogilby) and an orange spotted catshark a. rubiginosus (last, gomon & gledhill) (scyliorhinidae) from off southeastern queensland, australia. the key features for identification are: a pear-shaped trunk, longer than it is wide, along with a cephalothorax characterised by two large ventral papillae, projecting laterally and supporting ...200314707509
ascorbic acid concentration in cv. conference pears during fruit development and postharvest storage.l-ascorbic acid (l-aa) concentration changes during the development of cv. conference pears and the influence of postharvest handlings (gas condition, cooling rate, cooling duration) on l-aa breakdown were studied. l-aa concentration fluctuates in young fruits, remains stable during fruit maturation, and starts to decline 1 week before commercial harvest. the most rapid decrease in l-aa concentration was found during immediate controlled atmosphere. during short-term storage, only the gas condit ...200314705909
ampullary organs and electroreception in freshwater carcharhinus leucas.the ampulla of lorenzini of juvenile carcharhlinus leucas differ histologically from those previously described for other elasmobranchs. the wall of the ampullary canal consists of protruding hillock-shaped epidermal cells that appear to secrete large quantities of a mucopolysaccharide gel. the ampullary organs comprise a long canal sheathed in collagen terminating in an ampulla. each ampulla contains six alveolar sacs, with each sac containing hundreds of receptor cells. the receptor cells are ...200814692487
gorham stout syndrome (disappearing bone disease): two additional case reports and a review of the literature.gorham-stout syndrome, or disappearing bone disease, is characterized by the proliferation of thin-walled vascular channels associated with regional osteolysis. there have been fewer than 150 cases reported in the literature. in this clinical report, we describe 2 additional cases of gorham-stout syndrome affecting the maxillofacial skeleton. we provide a review of the clinical diagnosis of this syndrome and describe treatment options.200314676163
sources of polycyclic aromatic hydrocarbon exposure in non-occupationally exposed koreans.urinary 1-hydroxypyrene (1-ohp), an exposure biomarker for polycyclic aromatic hydrocarbons (pahs), was used to identify potential sources of pah exposure for 660 koreans who were not occupationally exposed to pahs (65% male; 35% female; mean age, 36.5 +/- 11.1 years). in this study, 74% of subjects had detectable levels of urinary 1-ohp, with a concentration range of 0.001-3.796 microg/l (median, 0.079 microg/l). a backward elimination was conducted: five variables were selected with a signific ...200314673870
morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo.the rapid development of the gastrointestinal tract posthatch has been described; however, little information exists concerning the development of the small intestine in the prehatch period. the present study examined the morphological, cellular, and molecular changes occurring in the small intestine toward the end of the incubation period by examining the expression of intestinal genes that code for brush border digestive enzymes and transporters, their biochemical activities, and the morpholog ...200314653469
urine mutagenicity and lymphocyte dna damage in fruit growers occupationally exposed to the fungicide captan.to determine haematological parameters, urine mutagenicity (on three salmonella typhimurium strains), and dna damage (using the comet assay) in mononuclear leucocytes of farmers before and after a one-day spraying period of pear and apple trees with the fungicide captan in usual conditions.200314634181
chemical and biochemical changes in prickly pears with different ripening behaviour.chemical and biochemical changes were studied in ripening prickly pears from three opuntia morphospecies with different ripening behaviour: naranjona (o. ficus-indica), blanca cristalina (opuntia sp.), and charola (o. streptacantha), of early, intermediate, and late ripening, respectively. at fullyripe stage (commercial maturity), blanca cristalina showed the biggest fruits, the hardest texture, and its pulp had the highest protein content. there were no significant differences among morphospeci ...200314609090
triterpenoids from the stembark of pyrus communis.three triterpenoids were isolated from the stem bark of pyrus communis linn. and identified as lup-20(29)-ene-3alpha, 27-diol (1), lup-20(29)-ene-3alpha-ol (2) and lup-20(29)-ene-3alpha, 28-diol (3) on the basis of spectral and chemical analysis. the compound (1) is a novel triterpene, being reported first time by us.200314577699
mri and x-ray ct study of spatial distribution of core breakdown in 'conference' pears.two non-destructive tomographic techniques, x-ray ct imaging and magnetic resonance imaging (mri), were applied to study the development of core breakdown disorder in 'conference' pears (pyrus communis cv. conference). this disorder, which is characterized by brown discoloration of the tissue and development of cavities, is induced by elevated co(2) and decreased o(2) levels during controlled atmosphere storage. tomographic images of pears stored for 10 months under disorder inducing conditions, ...200314559346
radiation therapy in the treatment of gorham disease.an adolescent male with noonan syndrome presented with a nonhealing fracture of the proximal right humerus. over a 7-month period there was progressive loss of bone in this region, resulting in a flail arm at 9 months. radiographic review was consistent with gorham disease. in addition, there was significant bleeding in the soft tissues and pain. radiation was delivered. seven years passed, until the patient re-presented with right hemithorax near collapse secondary to chylothorax. a chest tube ...200314528108
the influence of antibiotic production and pre-emptive colonization on the population dynamics of pantoea agglomerans (erwinia herbicola) eh1087 and erwinia amylovora in planta.stigma colonization by erwinia amylovora is the crucial first step in the development of most fire blight infections in apple and pear trees. suppression at this point of the disease process by antagonists of e. amylovora, such as pantoea agglomerans (erwinia herbicola) strain eh1087, is a rational approach to control fire blight. we tested the hypothesis that the ability of e. amylovora to compete with eh1087 for colonization of a stigma is reduced by the potential for eh1087 to produce the phe ...200314510856
light and electron microscopic studies of myxobolus stomum n. sp. (myxosporea: myxobolidae) infecting the blackspotted grunt plectorhynicus gaterinus (forsskal, 1775) in the red sea, egypt.a new myxosporean parasite, myxobolus stomum n. sp., is described from the oral cavity and lips of the blackspotted grunt plectorhynicus gaterinus (forsskal, 1775) in the red sea, egypt. the parasite was observed as tiny aggregates of whitish cysts hardly noticed within the muscles of the oral cavity, especially within the lips. the spores were subspherical and measured 8.5x6.5 microm. polar capsules were equal, pear-shaped, occupied about half of the spore length and measured 4.4x2.4 microm. hi ...200314505043
gorham disease of the cervical spine-a case report and review of the literature.study design a case report and literature review of gorham disease involving the cervical spine.200314501935
the gorham proverbs test. 196014428116
f. gorham brigham 1882-1964. 201414210695
the effect of anxiety as measured by the taylor mas scale on performance on the gorham proverb tests. 196314042968
[massive osteolysis of gorham-stout]. 196013841240
[studies on the phenol content of pyrus communis l. part 6. the isolation and characterization of a new phenol compound]. 196013825174
[findings on the phenolic content of pyrus communis. 5. occurrence of tannins in pear leaves]. 195813566981
[studies on the phenolic constituents of pyrus communis. iv. content of arbutin in germinating pear seeds & distribution in young plants]. 195813553772
[incidence of ursolic acid in the leaves of pyrus communis l]. 195813545850
[examination of phenolic content of pyrus communis. iii. effect of grafting on the arbutin content of the leaves of twigs & the base]. 195813542184
[studies on phenolic components of pyrus communis. ii. arbutin content of pear leaves]. 195713527184
[phenolic components of pyrus communis l. i. compilation of hitherto published data]. 195713494187
nelson gorham russell. 201513486596
the significance of poly-phenolic metabolites of apple and pear in the host relations of venturia inaequalis and venturia pirina. 195713481331
[inner hair cells isolation and morphological observation].to set up an effective technique for isolating the single inner hair cells (ihcs) and observe morphological features to distinguish ihcs from other hair cells (ohcs) in vitro.200212966811
apple and pear peel and pulp and their influence on plasma lipids and antioxidant potentials in rats fed cholesterol-containing diets.the aim of this study was to assess the bioactive compounds of apple and pear peel and pulp in vitro and their influence on plasma lipids and antioxidant potentials in vivo. the antioxidant potentials measured by 1,1-diphenyl-2-picrylhydrazyl (dpph), beta-carotene bleaching (beta-carotene), and nitric oxide inhibition radical scavenging (no) tests in apple peel and pulp were significantly higher than in pear peel and pulp, respectively. the ethanol extract of apple peels showed the strongest inh ...200312952433
chemical characterization and biological effects of sicilian opuntia ficus indica (l.) mill. fruit juice: antioxidant and antiulcerogenic activity.the juice of whole fruits of sicilian cultivars of prickly pear (opuntia ficus indica (l.) mill.) was investigated, and the contents of ascorbic acid, total polyphenols, and flavonoids were determined. in the juice, ferulic acid was the chief derivative of hydroxycinnamic acid and the mean concentration of total phenolic compounds was 746 microg/ml. the flavonoid fraction, analyzed by high-performance liquid chromatography-diode array detection, consisted of rutin and isorhamnetin derivatives. t ...200312903943
structure-function relationships affecting the insecticidal and miticidal activity of sugar esters.synthetic sugar esters are a relatively new class of insecticidal compounds that are produced by reacting sugars with fatty acids. the objective of this research was to determine how systematic alterations in sugar or fatty acid components of sugar ester compounds influenced their insecticidal properties. sucrose octanoate, sorbitol octanoate, sorbitol decanoate, sorbitol caproate, xylitol octanoate, xylitol decanoate and xylitol dodecanoate were synthesized and evaluated against a range of arth ...200312852599
qualitative and quantitative chromatographic investigation of flavonoids in pyrus communis l. flowers.the qualitative and quantitative analysis of flavonoids, and the quantitative determination of procyanidins in the flowers of naturally growing pyrus communis and in the flowers of cultivated varieties were carried out. the flavonoid compounds were investigated by chromatographic methods. the flavonoid samples were found in all the studied plant materials. the content of flavonoids was determined by the christ-müller's and hplc methods after acid hydrolysis. the quantitative determination of pro ...201312848372
the erwinia chrysanthemi phop-phoq operon plays an important role in growth at low ph, virulence and bacterial survival in plant tissue.we have studied the role of acidic ph as a barrier for the colonization of the plant apoplast by erwinia chrysanthemi. a minitransposon containing a promoterless reporter gene, gus, was used for random mutagenesis of the bacterial genome. an acid-sensitive mutant, named bt119, was isolated and had the following differential features with respect to the wild-type strain: (i) inability to grow at ph </= 5.5; (ii) decreased survival at acid ph and in plant tissues; (iii) increased susceptibility to ...200312828634
characterization of the first korean isolate of a chlamydia pneumoniae strain.chlamydia pneumoniae is a common pathogen that causes upper and lower respiratory tract infections and is difficult to isolate from clinical specimens. recently, we succeeded in isolating the first c. pneumoniae strain in korea. this study characterizes the morphology, infectivity, and drug sensitivity of the korean strain, designated lkk-1. electron microscopy was performed for thin sections, and the infectivity over time was tested by counting the inclusion-forming units every 12 h. the minimu ...200312824688
structure of the cryptosporidium parvum microneme: a metabolically and osmotically labile apicomplexan organelle.from an em study of thin sections, the rod-like microneme organelles within conventionally glutaraldehyde fixed cryptosporidium parvum sporozoites have been shown to undergo a shape change to a more spherical structure when the sporozoites age in vitro for a period of approximately 12 to 24 h. this correlates with the shape change of intact sporozoites, from motile hence viable thin banana-shaped cells to swollen pear-shaped cells, shown by differential interference contrast light microscopy of ...200312801539
influence of 1-methylcyclopropene on ripening, storage life, and volatile production by d'anjou cv. pear fruit.d'anjou cv. pear fruit (pyrus communis l.) exposed at harvest to 0, 0.42, 4.2, or 42 micromol m(-)(3) 1-methylcyclopropene (1-mcp) for 12 h at 20 degrees c were stored at 1 degrees c for up to 8 months. after storage, half of the fruit was continuously exposed to ethylene (0.45 or 4-18 mmol m(-)(3)) for 7 days at 20 degrees c. all fruit treated with 1-mcp had lower respiration and ethylene production compared to untreated controls. fruit quality changes were delayed following 1-mcp treatment, as ...200312797756
[heterogenicity of gorham-stout syndrome: association with lymphatic and venous malformations].gorham-stout syndrome is a rare disorder of unknown etiology characterized by osteolysis and microscopic proliferation of abnormal vessels. we report two cases of this syndrome associated with lymphatic and venous malformations.200312781118
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