Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influenza-like illness surveillance on the california-mexico border, 2004-2009. | please cite this paper as: kammerer et al. (2011). influenza-like illness surveillance on the california-mexico border, 2004-2009. influenza and other respiratory viruses doi: 10.1111/j.1750-2659.2011.00316.x. background since 2004, the naval health research center, with san diego and imperial counties, has collaborated with the us centers for disease control and prevention to conduct respiratory disease surveillance in the us-mexico border region. in 2007, the secretariat of health, mexico and ... | 2011 | 22212638 |
| the prevalance of respiratory viruses among healthcare workers serving pilgrims in makkah during the 2009 influenza a (h1n1) pandemic. | despite the high risk of acquiring respiratory infections, healthcare workers who treat pilgrims at hajj have not been studied in previous research on respiratory diseases during hajj. the objective of this study was to determine the prevalence of different respiratory viruses among healthcare workers who treated pilgrims during hajj 2009, the year of the influenza a h1n1 pandemic. a cross-sectional study was performed just before and after hajj (25-29 november, 2009). nasal and throat swabs wer ... | 2011 | 22197024 |
| acute infantile encephalopathy predominantly affecting the frontal lobes (aief). | acute infantile encephalopathy predominantly affecting the frontal lobes (aief) is a relatively recent described entity. this article includes case reports of two patients who had bifrontal involvement during acute febrile encephalopathy. case 1 describes a 1-y-old boy who presented with hyperpyrexia and dialeptic seizures. imaging revealed significant bilateral frontal lobe involvement while serology proved presence of influenza b infection. over a period of one wk, he recovered with significan ... | 2012 | 22183762 |
| vitamin d supplementation does not increase immunogenicity of seasonal influenza vaccine in hiv-infected adults. | the influence of vitamin d on influenza vaccine immunogenicity in hiv was assessed using data from a phase 3, randomized trial conducted during the 2008-2009 influ-enza season. thirty-three percent of participants were on supplemental vitamin d at baseline. neither seroconversion nor seroprotection were predicted by vitamin d use for any of the 3 vaccine strains. there is no evidence of improved influenza vaccine immunogenicity with vitamin d supplementation in this hiv-positive population. | 2011 | 22180525 |
| [Etiology survey on virus of acute respiratory infection in Guangzhou from 2006 to 2009]. | To investigate the pathogens of acute respiratory infection (ARI) in Guangzhou from 2006 to 2009. | 2011 | 22177307 |
| The epidemiology of pediatric patients seen at the Children's Hospital of Oklahoma with laboratory confirmed influenza in 2009. | In 2009, a new strain of influenza A, subtype H1N1, infected otherwise healthy children with increased rate of hospitalization. | 2011 | 22164573 |
| update: influenza activity--united states, october 2-november 26, 2011. | during october 2-november 26, 2011, influenza activity remained low in the united states. thus far, influenza a viruses have predominated, and the majority are antigenically related to the 2011-12 influenza vaccine strains for the northern hemisphere. this report summarizes u.s. influenza activity since october 2 and updates the previous summary. | 2011 | 22157883 |
| the efficacy of intranasal live attenuated influenza vaccine in children 2 through 17 years of age: a meta-analysis of 8 randomized controlled studies. | nine randomized controlled clinical trials, including approximately 26,000 children aged 6 months to 17 years, have evaluated the efficacy of live attenuated influenza vaccine (laiv) against culture-confirmed influenza illness compared with placebo or trivalent inactivated influenza vaccine (tiv). the objective of the current analysis was to integrate available laiv efficacy data in children aged 2-17 years, the group for whom laiv is approved for use. | 2012 | 22155144 |
| [Comparative immunogenicity studies of cultural and peptide influenza vaccines]. | Comparative immunogenicity studies of experimental vaccines based onA/Aichi/2/68 neuraminidase peptide fragments (NA) and influenza virus A and B strains produced in MDCK cell culture. | 2011 | 22145349 |
| global monitoring of antiviral resistance in currently circulating human influenza viruses, november 2011. | 2011 | 22128380 | |
| preparation and characterization of egg yolk immunoglobulin y specific to influenza b virus. | the aim of this study was to prepare egg yolk immunoglobulin (igy) for use in the prevention and treatment of influenza b viral infections. laying hens were immunized with inactivated influenza b virus (ibv), and igy was isolated from the egg yolk by multiple polyethylene glycol (peg) 6000 extraction and ammonium sulfate purification steps. the titers and specificity of the purified antibodies were assessed. the specific igy titer increased beginning the second week after the first immunization, ... | 2012 | 22127067 |
| Palindromes drive the re-assortment in Influenza A. | Different subtypes of Influenza A virus are associated with species specific, zoonotic or pandemic Influenza. The cause of its severity underlies in complicated evolution of its segmented RNA genome. Although genetic shift and genetic drift are well known in the evolution of this virus, we reported the significant role of unique RNA palindromes in its evolution. Our computational approach identified the existence of unique palindromes in each subtype of Influenza A virus with its absence in Infl ... | 2011 | 22125380 |
| isolation of influenza viruses in mdck 33016pf cells and clearance of contaminating respiratory viruses. | this paper summarizes results obtained by multiplex pcr screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in mdck 33016pf cells. using the resplexii v2.0 (qiagen) multiplex pcr, 393 positive results were obtained in 468 clinical samples collected during an influenza season in germany. the overall distribution of positive results was influenza a 42.0%, influenza b 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronaviru ... | 2012 | 22119922 |
| respiratory viruses transmission from children to adults within a household. | the aim of this study was to examine the rate of transmission of influenza and other respiratory viruses from children attending an emergency department to their family members in the household using active surveillance. | 2012 | 22119589 |
| applicability of a sensitive duplex real-time pcr assay for identifying b/yamagata and b/victoria lineages of influenza virus from clinical specimens. | type b influenza virus is one of the major epidemic strains and responsible for considerable mortality and morbidity. rapidly and accurately identifying different influenza b virus lineages, i.e., b/yamagata (b/y) and b/victoria (b/v), is desirable during the flu season. however, the available rapid techniques lack sensitivity, and the usual methods for identifying influenza viruses require expansion of virus in tissue culture or embryonated hen's eggs. thus, we developed several sets of primer ... | 2011 | 22113559 |
| semi-quantitative analysis of influenza samples using the luminex xtag(®) respiratory viral panel kit. | the luminex xtag(®) respiratory viral panel (rvp) kit simultaneously detects and identifies multiple respiratory viruses including several subtypes of influenza a using a multiplex nucleic acid amplification test assay platform. the emitted fluorescence signal from the rvp assay provides qualitative information on the presence of a particular viral species in respiratory specimens. however, a quantitative assessment is preferred when monitoring environmental samples for respiratory viruses. in t ... | 2011 | 22112187 |
| i-move multi-centre case control study 2010-11: overall and stratified estimates of influenza vaccine effectiveness in europe. | in the third season of i-move (influenza monitoring vaccine effectiveness in europe), we undertook a multicentre case-control study based on sentinel practitioner surveillance networks in eight european union (eu) member states to estimate 2010/11 influenza vaccine effectiveness (ve) against medically-attended influenza-like illness (ili) laboratory-confirmed as influenza. | 2011 | 22110695 |
| comparative analyses of pandemic h1n1 and seasonal h1n1, h3n2, and influenza b infections depict distinct clinical pictures in ferrets. | influenza a and b infections are a worldwide health concern to both humans and animals. high genetic evolution rates of the influenza virus allow the constant emergence of new strains and cause illness variation. since human influenza infections are often complicated by secondary factors such as age and underlying medical conditions, strain or subtype specific clinical features are difficult to assess. here we infected ferrets with 13 currently circulating influenza strains (including strains of ... | 2011 | 22110664 |
| acute respiratory distress syndrome caused by influenza b virus infection in a patient with diffuse large b-cell lymphoma. | influenza b virus infections are less common than infections caused by influenza a virus in critically ill patients, but similar mortality rates have been observed for both influenza types. pneumonia caused by influenza b virus is uncommon and has been reported in pediatric patients and previously healthy adults. critically ill patients with pneumonia caused by influenza virus may develop acute respiratory distress syndrome. we describe the clinical course of a critically ill patient with diffus ... | 2011 | 22110513 |
| influenza b viruses with mutation in the neuraminidase active site, north carolina, usa, 2010-11. | oseltamivir is 1 of 2 antiviral medications available for the treatment of influenza b virus infections. we describe and characterize a cluster of influenza b viruses circulating in north carolina with a mutation in the neuraminidase active site that may reduce susceptibility to oseltamivir and the investigational drug peramivir but not to zanamivir. | 2011 | 22099093 |
| false positive pcr results linked to administration of seasonal influenza vaccine. | false positive pcrs occur as a consequence of specimen to specimen or amplicon to specimen contamination within the laboratory. evidence of a novel contamination at time of specimen collection linked to influenza vaccine administration in the same location as influenza sampling is described. clinical, circumstantial and laboratory evidence was gathered for each of 5 cases of influenza like illness (ili) with unusual pcr patterns of positivity for seasonal h1n1, h3n2, h1n1 (2009), and influenza b ... | 2011 | 22096134 |
| standardization of denaturing gradient gel electrophoresis (dgge) for discrimination of victoria and yamagata lineages of influenza b. | in this study, a pcr-dgge protocol was standardized in order to distinguish victoria and yamagata influenza b lineages directly from clinical samples. after routine multiplex pcr characterization, amplicons of the haemagglutinin gene bearing a 40bp-length gc clamp were generated by nested-pcr and analyzed by electrophoresis in 6% polyacrylamide gel with a 25-45% urea-formamide gradient. the results showed a perfect correlation between dgge and phylogenetic analyses for all compared samples, besi ... | 2012 | 22079619 |
| influenza viruses in thailand: 7 years of sentinel surveillance data, 2004-2010. | the re-emergence of avian influenza a (h5n1) in 2004 and the pandemic of influenza a (h1n1) in 2009 highlight the need for routine surveillance systems to monitor influenza viruses, particularly in southeast asia where h5n1 is endemic in poultry. in 2004, the thai national institute of health, in collaboration with the u.s. centers for disease control and prevention, established influenza sentinel surveillance throughout thailand. | 2012 | 22074057 |
| safety and immunogenicity of a new trivalent inactivated split-virus influenza vaccine in healthy korean children: a randomized, double-blinded, active-controlled, phase iii study. | we report results of a randomized, double-blinded, active-controlled, phase iii study conducted to evaluate the immunogenicity and safety of a new trivalent inactivated split-virus influenza vaccine (gc501) manufactured by the green cross corporation in korea. a total of 283 healthy children aged 6 months to < 18 yr were randomized to receive either gc501 or control. of the gc501 recipients, seroconversion occurred in 48.5% for a/h1n1, 67.7% for a/h3n2 and 52% for influenza b. the proportion of ... | 2011 | 22065897 |
| antibody persistence and response to 2010-2011 trivalent influenza vaccine one year after a single dose of 2009 as03-adjuvanted pandemic h1n1 vaccine in children. | in 2009, several countries used the aso3-adjuvanted pandemic a/h1n1 vaccine. we assessed the persistence of antibody and the priming induced by a single paediatric dose of this vaccine in children. | 2011 | 22063386 |
| effectiveness of pandemic h1n1-2009 vaccination in reducing laboratory confirmed influenza infections among military recruits in tropical singapore. | limited information is available about pandemic h1n1-2009 influenza vaccine effectiveness in tropical communities. we studied the effectiveness of a pandemic h1n1 vaccination program in reducing influenza cases in singapore. | 2011 | 22053196 |
| recommended composition of influenza vaccines for use in the 2012 southern hemisphere influenza season. | 2011 | 22046594 | |
| occurrence of influenza among patients hospitalized for suspicion of influenza a (h1n1) infection in 2010 at a sentinel hospital in são paulo, brazil. | in 2010, 96 patients suspected of being infected with the influenza a (h1n1) virus were hospitalized at the hospital são paulo, located in the city of são paulo, brazil. of those 96 patients, 4 (4.2%) were found to be infected with influenza a virus-3 with influenza a (h1n1) and 1 with seasonal influenza a-and 2 patients (2.1%) were found to be infected with influenza b virus. most (63.5%) of the suspected cases occurred in children, as did half of the positive cases. the second wave of influenz ... | 2011 | 22042398 |
| impact of influenza during the post-pandemic season: epidemiological picture from syndromic and virological surveillance. | following the observation that 1 or 2 pandemic peak due to the circulation ofahinlv had occurred in most countries and in most world health organization (who) regions, who declared on august 10"h, 2010 that the world was moving into the post-pandemic period, whose surveillance presents considerable interest both from epidemiological and clinical point of view. we described the epidemiological picture emerged from syndromic and virological surveillance during the post-pandemic season in liguria, ... | 2011 | 22010543 |
| comparison of the filmarray respiratory panel and prodesse real-time pcr assays for detection of respiratory pathogens. | we compared the diagnostic performance and overall respiratory pathogen detection rate of the premarket version of the filmarray respiratory panel (rp) multiplex pcr assay (idaho technology, inc., salt lake city, ut) with those of the food and drug administration (fda)-cleared prodesse proflu+, profast+, proparaflu+, pro hmpv+, and proadeno+ real-time pcr assays (gen-probe, san diego, ca). the assays were performed on a panel of 192 nasopharyngeal-secretion specimens collected from 81 children u ... | 2011 | 21998418 |
| Clinical and socioeconomic impact of different types and subtypes of seasonal influenza viruses in children during influenza seasons 2007/2008 and 2008/2009. | There are few and debated data regarding possible differences in the clinical presentations of influenza A/H1N1, A/H3N2 and B viruses in children. This study evaluates the clinical presentation and socio-economic impact of laboratory-confirmed influenza A/H1N1, A/H3N2 or B infection in children attending an Emergency Room because of influenza-like illness. | 2011 | 21992699 |
| Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. | Please cite this paper as: Allard et al. (2011) Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00297.x. Background Shared seasonal patterns, such as between influenza and some respiratory bacterial infections, can create associations between phenomena not causally related. Objectives To estimate the association of influenza with subsequent bacterial infections after ... | 2011 | 21985083 |
| Laninamivir octanoate: a new long-acting neuraminidase inhibitor for the treatment of influenza. | Oseltamivir and zanamivir are well-established and well-researched drugs for the treatment of influenza in Japan and the rest of the world. A new neuraminidase inhibitor, laninamivir octanoate, has been approved for use in Japanese clinics. Laninamivir octanoate is an inhaled drug with unique characteristics. The inhaled laninamivir octanoate is converted into its active form, laninamivir, in the lungs where a high concentration persists for a long period of time. The concentration of laninamivi ... | 2011 | 21973296 |
| Make new friends, but keep the old: influenza vaccines in children with cancer. | 2011 | 21949044 | |
| safety and immunogenicity of live attenuated and inactivated influenza vaccines in children with cancer. | the safety and immunogenicity of live, attenuated influenza vaccine (laiv) has not been compared to that of the standard trivalent inactivated vaccine (tiv) in children with cancer. | 2011 | 21949042 |
| receptor tyrosine kinase inhibitors that block replication of influenza a and other viruses. | we have previously reported that two receptor tyrosine kinase inhibitors (rtkis), called ag879 and tyrphostin a9 (a9), can each block the replication of influenza a virus in cultured cells. in this study, we further characterized the in vitro antiviral efficacies and specificities of these agents. the 50% effective concentration (ec(50)) of each against influenza a was found to be in the high nanomolar range, and the selectivity index (si = 50% cytotoxic concentration [cc(50)]/ec(50)) was determ ... | 2011 | 21930873 |
| In vitro and in vivo efficacy of fluorodeoxycytidine analogs against highly pathogenic avian influenza H5N1, seasonal, and pandemic H1N1 virus infections. | Various fluorodeoxyribonucleosides were evaluated for their antiviral activities against influenza virus infections in vitro and in vivo. Among the most potent inhibitors was 2'-deoxy-2'-fluorocytidine (2'-FdC). It inhibited various strains of low and highly pathogenic avian influenza H5N1 viruses, pandemic H1N1 viruses, an oseltamivir-resistant pandemic H1N1 virus, and seasonal influenza viruses (H3N2, H1N1, influenza B) in MDCK cells, with the 90% inhibitory concentrations ranging from 0.13 to ... | 2011 | 21925541 |
| Update: influenza activity--United States and worldwide, May 22-September 3, 2011. | During May 22-September 3, 2011, the United States experienced low levels of influenza activity; 2009 influenza A (H1N1), influenza A (H3N2), and influenza B viruses were detected worldwide and identified sporadically in the United States. Typical seasonal patterns of influenza activity occurred in the Southern Hemisphere. This report summarizes influenza activity in the United States and worldwide since the last update. | 2011 | 21918493 |
| Influenza-associated pediatric deaths--United States, September 2010-August 2011. | Influenza-associated pediatric mortality has been a nationally notifiable condition since October 2004. This report summarizes the 115 cases of influenza-associated pediatric mortality reported to CDC that occurred from September 1, 2010, through August 31, 2011. Deaths occurred in 33 states. Nearly half of the deaths (46%) occurred in children aged <5 years. Of the children who died, 49% had no known Advisory Committee on Immunization Practices (ACIP)-defined high-risk medical conditions, and 3 ... | 2011 | 21918492 |
| the dba.2 mouse is susceptible to disease following infection with a broad, but limited, range of influenza a and b viruses. | we assessed the relative susceptibilities to disease of the dba.2 and c57bl/6 mouse models upon infection with a range of influenza a and b viruses. dba.2 mice were more susceptible to disease upon inoculation with human h1n1 influenza a virus strains, several swine influenza viruses, and influenza b viruses but were not overtly susceptible to infection with human seasonal h3n2 strains. hemagglutination inhibition and immunoglobulin isotype profiling indicated that dba.2 and c57bl/6 mice generat ... | 2011 | 21917963 |
| influenza b virus with modified hemagglutinin cleavage site as a novel attenuated live vaccine. | both pandemic and interpandemic influenza is associated with high morbidity and mortality worldwide. seasonal epidemics are caused by both influenza a and b virus strains that cocirculate with varying predominance and may give rise to severe illness equally. according to world health organization recommendations, current annual vaccines are composed of 2 type a and 1 type b virus-specific component. | 2011 | 21917878 |
| trivalent and quadrivalent mf59(®)-adjuvanted influenza vaccine in young children: a dose- and schedule-finding study. | young children are at increased risk for influenza infections and related complications. the protection offered to children by conventional trivalent inactivated influenza vaccines (tiv) is suboptimal, due to poor immunogenicity and a higher exposure to infection and complications in this age group, particularly from influenza b strains. in this dose-ranging, factorial design trial, we report the safety and immunogenicity of different combinations of adjuvanted (ativ) and non-adjuvanted trivalen ... | 2011 | 21906647 |
| Detection of an influenza B virus strain with reduced susceptibility to neuraminidase inhibitor drugs. | 2011 | 21900523 | |
| influenza disease burden in adults by subtypes following the initial epidemic of pandemic h1n1 in singapore. | peaks of influenza activity in july 2009 and january 2010 were >90% pandemic h1n1 (ph1n1), but by may 2010, h3n2 predominated in hospital attendances (46·5%, versus 38·9% ph1n1); h3n2 hospital attendances were older (72·9% aged ≥60 years versus 13·5% for ph1n1), but the age-stratified proportions admitted for pneumonia ]were similar. as at the end of the third epidemic wave in singapore, ph1n1 cases in hospital attendances were still markedly younger than cases of h3n2 or influenza b, with littl ... | 2011 | 21883962 |
| High and continuous exposure of laninamivir, an anti-influenza drug, may work suppressively to generate low-susceptibility mutants in animals. | Laninamivir octanoate (Inavir(®); Daiichi Sankyo, Tokyo, Japan) is an anti-influenza drug that provides complete treatment by a single inhalation. It works as a long-acting neuraminidase (NA) inhibitor by means of high and continuous exposure of laninamivir, its active metabolite, in the lungs of mice after intranasal administration. Even after 6 days after intranasal administration of 236 µg/kg laninamivir octanoate, the concentration of laninamivir in the lungs was maintained more than 2-3 ord ... | 2011 | 21881920 |
| Severe influenza cases in paediatric intensive care units in Germany during the pre-pandemic seasons 2005 to 2008. | Data on complications in children with seasonal influenza virus infection are limited. We initiated a nation-wide three-year surveillance of children who were admitted to a paediatric intensive care unit (PICU) with severe seasonal influenza. | 2011 | 21880125 |
| missed diagnosis of influenza b virus due to nucleoprotein sequence mutations, singapore, april 2011. | a new influenza b variant was discovered in singapore in april 2011 during diagnostic testing of a 3-year-old boy with respiratory symptoms. influenza b virus was isolated from culture and confirmed by standard immunofluorescence testing, but was not detected by the routine, in-house influenza screening reverse-transcription polymerase chain reaction assay that targets the nucleoprotein (np) gene. subsequent sequencing investigations demonstrated that several other published assays targeting np ... | 2011 | 21871229 |
| Prevention and control of influenza with vaccines: recommendations of the Advisory Committee on Immunization Practices (ACIP), 2011. | This document provides updated guidance for the use of influenza vaccines in the United States for the 2011-12 influenza season. In 2010, the Advisory Committee on Immunization Practices (ACIP) first recommended annual influenza vaccination for all persons aged =6 months in the United States. Vaccination of all persons aged =6 months continues to be recommended. Information is presented in this report regarding vaccine strains for the 2011-12 influenza season, the vaccination schedule for childr ... | 2011 | 21866086 |
| rna viruses in young nepalese children hospitalized with severe pneumonia. | pneumonia is among the leading causes of illness and death in children <5 years of age worldwide. there is little information on the viral etiology of severe pneumonia in low-income countries, where the disease burden is particularly high. | 2011 | 21860338 |
| influenza drug resistance. | influenza viruses are major human pathogens with a global distribution, accounting for more than 500,000 annual deaths worldwide and with considerable impact on the quality of life and productivity of the society. due to the limited efficacy of vaccination, antiviral drugs constitute a complementary approach in the control and prevention of influenza infections and thus play an important role in the management of influenza outbreaks and pandemics. currently, adamantanes and neuraminidase inhibit ... | 2011 | 21858746 |
| influenza: epidemiology, clinical features, therapy, and prevention. | influenza a and b are important causes of respiratory illness in all age groups. influenza causes seasonal outbreaks globally and, less commonly, pandemics. in the united states, seasonal influenza epidemics account for >200,000 hospitalizations and >30,000 deaths annually. more than 90% of deaths occur in the elderly population. interestingly, in the novel 2009 h1n1 influenza pandemic, attack rates were highest among children and young adults. fewer than 10% of cases occurred in adults >60 year ... | 2011 | 21858743 |
| Influenza ?/Victoria antigen induces strong recall of ?/Yamagata but lower ?/Victoria response in children primed with two doses of ?/Yamagata. | Trivalent inactivated influenza vaccine (TIV) contains 1 of 2 influenza B/lineages (B/Yamagata or B/Victoria) annually. We assessed prime-boost responses in young children following a change in the B/lineage included in TIV. | 2011 | 21857263 |
| immunogenicity and safety of intradermal influenza vaccine in children. | in order to compare the immunogenicity and safety of different doses of trivalent influenza vaccine (tiv) administered intradermallly (id) with those evoked by a full dose of intramuscular (im) virosomal-adjuvanted influenza vaccine (va-tiv), 112 previously primed healthy children aged ≥ 3 years were randomised to receive 9 μg or 15 μg of each strain of id-tiv, or a full im dose (15 μg of each strain) of va-tiv. the a/h1n1 and a/h3n2 seroconversion and seroprotection rates were ≥ 90% and geometr ... | 2011 | 21855592 |
| protective efficacy of live-attenuated influenza vaccine (multivalent, ann arbor strain): a literature review addressing interference. | selecting the b strain for inclusion in a trivalent seasonal influenza vaccine has been difficult because two distinct influenza b lineages frequently co-circulate, prompting consideration of a quadrivalent vaccine containing two a and two b strains. because interference among wild-type influenza viruses is a well-documented phenomenon and viral replication is required to elicit protection by the licensed live-attenuated influenza vaccine (laiv; medimmune, llc, gaithersburg, md, usa), a potentia ... | 2011 | 21854309 |
| first influenza season after the 2009 pandemic influenza: characteristics of intensive care unit admissions in adults and children in vall d'hebron hospital. | clin microbiol infect abstract: to assess potential differences in epidemiology and management of patients admitted with influenza infection in the intensive care unit (icu) during the first post-pandemic influenza period. observational prospective study comparing september 2009-january 2010 with september 2010-january 2011. variables captured: demographics, co-morbidities, physiological parameters, outcomes and management. analysis was performed using spss v. 13.0; significance was set at p 0.5 ... | 2011 | 21851487 |
| antimycotic-antibiotic amphotericin b promotes influenza virus replication in cell culture. | in general, antibiotics are not rated as substances that inhibit or support influenza virus replication. we describe here the enhancing effect of the polyene antibiotic amphotericin b (amb) on influenza virus growth in vero cells. we show that isolation rates of influenza a and b viruses from clinical samples can be dramatically enhanced by adding amb to the culture medium. we demonstrate that amb promotes the viral uptake and endocytic processing of the virus particles. this effect is specific ... | 2011 | 21849438 |
| efficacy of single intravenous injection of peramivir against influenza b virus infection in ferrets and cynomolgus macaques. | we evaluated the efficacy of single intravenous dose peramivir for treatment of influenza b virus infection in ferrets and cynomolgus macaques in the present study. a single dose of peramivir (30 and 60 mg/kg) given to ferrets on 1 day post-infection with influenza b virus significantly reduced virus titers in nasal washes and total virus shedding as measured by areas under the curve (auc) by 2 log(10) compared with control group. furthermore, nasal virus titers on day 2 post infection and aucs ... | 2011 | 21844317 |
| reduced antibody responses to the pandemic (h1n1) 2009 vaccine after recent seasonal influenza vaccination. | the vaccination program against the 2009 pandemic h1n1 influenza (2009 h1n1) provided a unique opportunity to determine if immune responses to the 2009 h1n1 vaccine were affected by a recent, prior vaccination against seasonal influenza. in the present study, we studied the immune responses to the 2009 h1n1 vaccine in subjects who either received the seasonal influenza vaccination within the prior three months or did not. following 2009 h1n1 vaccination, subjects previously given a seasonal infl ... | 2011 | 21813667 |
| structural basis for the sequence-specific recognition of human isg15 by the ns1 protein of influenza b virus. | interferon-induced isg15 conjugation plays an important antiviral role against several viruses, including influenza viruses. the ns1 protein of influenza b virus (ns1b) specifically binds only human and nonhuman primate isg15s and inhibits their conjugation. to elucidate the structural basis for the sequence-specific recognition of human isg15, we determined the crystal structure of the complex formed between human isg15 and the n-terminal region of ns1b (ns1b-ntr). the ns1b-ntr homodimer intera ... | 2011 | 21808041 |
| a neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza a hemagglutinins. | the isolation of broadly neutralizing antibodies against influenza a viruses has been a long-sought goal for therapeutic approaches and vaccine design. using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (ha) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza a viruses. passive transfer of this antibody conferred protection to mice and ferrets. comple ... | 2011 | 21798894 |
| synthesis, antiviral and cytotoxic investigation of 2-phenyl-3-substituted quinazolin-4(3h)-ones. | a series of 3(benzylideneamino)-2-phenyl quinazoline-4(3h)-ones was synthesized by reaction of 3-amino-2-phenyl-3h-quinazoline-4-one with various carbonyl compounds. | 2011 | 21796872 |
| addition of the immunostimulatory oligonucleotide imt504 to a seasonal flu vaccine increases hemagglutinin antibody titers in young adult and elder rats, and expands the anti-hemagglutinin antibody repertoire. | flu vaccines are partially protective in infants and elder people. new adjuvants such as immunostimulatory oligonucleotides (odns) are strong candidates to solve this problem, because a combination with several antigens has demonstrated effectiveness. here, we report that imt504, the prototype of a major class of immunostimulatory odns, is a potent adjuvant of the influenza vaccine in young adult and elderly rats. flu vaccines that use virosomes or whole viral particles as antigens were combined ... | 2011 | 21793787 |
| [analysis on the etiological surveillance of influenza/novel influenza a (h1n1) from 2009-2010 in shanxi province]. | to study the epidemical characteristics of influenza/novel influenza a (h1n1) in shanxi province from 2009 to 2010, and to provide scientific foundations for predicting and controlling the pandemic outbreak of influenza/novel influenza a (h1n1) effectively. | 2011 | 21789846 |
| [restriction analysis of genome composition of live influenza vaccine]. | live attenuated cold-adapted influenza vaccine (liv) has been used in russia for over 50 years and proved to be safe and effective. currently, russian reassortant laiv is based on influenza aileningrad/134/17/57 (h2n2) and b/ussr/60/69 master donor viruses (mdvs) which are cold-adapted (ca), temperature-sensitive (ts), and attenuated (att), respectively. the mdvs are used to generate attenuated reassortant vaccine viruses containing the surface antigens of current wild type (wt) influenza a (hin ... | 2011 | 21786624 |
| [monoclonal antibodies with high virus-neutralizing activity against pandemic influenza virus a/llv-moscow/01/2009 (h1n1)swl]. | the authors have obtained a panel of 7 monoclonal antibodies (mabs) against pandemic influenza virus a/iiv-moscow/01/2009 (hin1)swl isolated in russia. one mab is directed to a np protein linear epitope and interacts with all the influenza a viruses under study. six other mabs are directed to h1 hemagglutinin conformation-dependent determinants and detect homologous virus in the hemagglutination-inhibition test, enzyme immunoassay, immunofluorescence and virus neutralization tests. mabs differen ... | 2011 | 21786621 |
| [taxonomic structure of orthomyxoviridae: current views and immediate prospects]. | analysis of taxonomic structure of orthomyxoviridae was undertaken in view of its anticipated evolution. four concepts of circulation of influenza a viruses in the biosphere are discussed, viz. anthrponose, zooanthroponose, metastrongilose, and protozoan. all of them may be considered in the framework of the general zooantroponose concept. influenza b and c viruses can not be regarded as strictly anthroponose. comparative molecular-genetic analysis of the genus thogotovirus provides a basis for ... | 2011 | 21786592 |
| retrospective and prospective verification of the cepheid xpert flu assay. | we performed a retrospective (n=121) and prospective (n=305) verification of the cepheid xpert flu assay to determine its performance characteristics. the overall sensitivity and specificity were 93% and 100%, respectively. nasopharyngeal specimen sensitivities were 100% for seasonal influenza a/h1 and influenza a/h3, 90% for influenza a/2009/h1n1, and 95% for influenza b. | 2011 | 21775544 |
| [the effects of a hot water soluble extract (s-03) isolated from isatis indigotica root on influenza a and b viruses in vitro]. | this study was to investigate the antiviral effects of a hot water soluble extract s-03 isolated from isatis indigotica root on different subtypes of influenza a and b viruses in mdck cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (had) assays. chemical analysis of the extract s-03 showed that it contained high proportion of polysaccharides. the antiviral effects in vitro showed that the s-03 had no effect on different influenza viruses if the drug wa ... | 2011 | 21774246 |
| influenza vaccine effectiveness in wisconsin during the 2007-08 season: comparison of interim and final results. | background: during the 2007-08 influenza season, we reported an interim vaccine effectiveness (ve) estimate of 44% for preventing medically attended influenza. in this analysis we report results for the entire season and compare them with the interim estimate. methods: patients with feverishness, chills, or cough <8 days duration were prospectively recruited over 10 weeks and tested for influenza by real-time reverse transcriptase pcr (rrt-pcr). case-control analyses were performed using data fr ... | 2011 | 21767593 |
| evolution of influenza viral neuraminidase (na) revealed by large-scale sequence analysis. | 2011 | 21761592 | |
| crystal structure of human isg15 in complex with influenza b virus ns1b. | isg15 (interferon-stimulated gene 15), the first ubiquitin-like protein (ubl) identified, has emerged as an important cellular antiviral factor. it consists of two ubl domains with a short linker between them. the covalent attachment of isg15 to host and viral proteins to modify their functions, similar to ubiquitylation, is named as isgylation. influenza b virus ns1b protein antagonizes human but not mouse isgylation because ns1b exhibits species-specificity: it only binds human and non-human p ... | 2011 | 21757684 |
| a large outbreak of influenza b-associated benign acute childhood myositis in germany, 2007/2008. | background:: benign acute childhood myositis (bacm) is a rare syndrome associated with various viral infections. bilateral calve pain may lead to inability to walk. during winter 2007/2008, we investigated a nationwide outbreak of influenza-associated bacm (ia-bacm) to identify etiologic (sub)type, describe the course of disease, and explore how well the syndrome is known among physicians. methods:: we performed retrospective and prospective case finding in all german federal states. physicians ... | 2011 | 21753259 |
| discovery of dihydro-alkyloxy-benzyl-oxopyrimidines as promising anti-influenza virus agents. | a series of novel dihydro-alkyloxy-benzyl-oxopyrimidine (dabo) derivatives were synthesized and evaluated for their activity against influenza virus in mdck cells. four dabo derivatives (4a1, 4a2, 4a3, and 4d1) showed potent activity against influenza virus. among them, compound 4a3 was the most promising lead with broad activity against influenza a (antiviral ec(50) values of 9 and 18 µm for the a/h1n1 and a/h3n2 subtype, respectively) and influenza b virus (ec(50) : 33 µm). the antiviral mecha ... | 2011 | 21752202 |
| predicting the epidemic sizes of influenza a/h1n1, a/h3n2, and b: a statistical method. | the epidemic sizes of influenza a/h3n2, a/h1n1, and b infections vary from year to year in the united states. we use publicly available us centers for disease control (cdc) influenza surveillance data between 1997 and 2009 to study the temporal dynamics of influenza over this period. | 2011 | 21750666 |
| infection control implications of influenza a and influenza b: coinfection or cocirculating strains? | 2011 | 21741728 | |
| comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-pcr assays. | many acute respiratory illness surveillance systems collect and test nasopharyngeal (np) and/or oropharyngeal (op) swab specimens, yet there are few studies assessing the relative measures of performance for np versus op specimens. | 2011 | 21738731 |
| self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses. | the gold standard for respiratory virus testing is a nasopharyngeal (np) swab, which is collected by a healthcare worker. midturbinate (mt) swabs are an alternative due to their ease of collection and possible self-collection by patients. the objective of this study was to compare the respiratory virus isolation of flocked mt swabs compared to flocked np swabs. | 2011 | 21731708 |
| hypomanic switching during influenza infection without intracranial infection in an adolescent patient with bipolar disorder. | manic switching, which is mood changing from depression to mania, induced by intracranial viral infections have been previously reported. however, manic switching induced by influenza infection without intracranial viral infection in a patient with bipolar disorder (bd), has not been previously reported. we report a patient with adolescent bd who showed a first episode of hypomanic switching during influenza b infection without intracranial viral infection. in addition to neurological monitoring ... | 2011 | 21730804 |
| effects of theramax on influenza virus infections in cell culture and in mice. | limited in vivo studies in the scientific literature suggest that components of green tea and elderberry may be beneficial in treating influenza virus infections. they are thought to act by blocking virus adsorption to cells. theramax(-«) is a proprietary medication administered by nasal spray that contains both green tea and elderberry extracts that was evaluated for antiviral activity. | 2011 | 21730370 |
| influenza virus-like particle can accommodate multiple subtypes of hemagglutinin and protect from multiple influenza types and subtypes. | despite existing vaccines and specific therapies, epidemics of seasonal influenza annually claim 200,000-500,000 lives worldwide. pandemic influenza represents an even greater threat, with numerous potentially pandemic viruses circulating in nature. development of multi-specific vaccines against multiple pandemic or seasonal strains is important for human health and the global economy. here we report a novel virus-like particle (vlp) platform that contains three hemagglutinin (ha) subtypes. this ... | 2011 | 21723354 |
| performance of leptospira immunoglobulin m elisa and rapid immunoglobulin g immunochromatographic assays for the diagnosis of leptospirosis. | using archived samples, we assessed the diagnostic capacity of two commercially available tests (an elisa igm and a rapid immunochromatographic test, ict) for the detection of leptospira spp. igm and igg antibodies to aid with the diagnosis of acute leptospirosis in febrile patients in thailand. the sensitivities of the elisa for the detection of igm and the rapid immunochromatographic test for the detection of igg were 60.7% (95% ci, 50.3 to 70.2%), and 83.2% (95% ci 73.9 to 89.6%) respectively ... | 2011 | 21721448 |
| surveillance and molecular characterization of human influenza b viruses during 2006-2010 revealed co-circulation of yamagata-like and victoria-like strains in eastern india. | acute respiratory illness (ari) is one of the major health problems in tropical countries of asia, like india where approximately 0.5million children in the age group of <5years die annually. previously we have reported the genetic characterization of influenza a (inf-a) strains circulating in kolkata, eastern india. this study was initiated to characterize the genetic diversity of the circulating influenza b (inf-b) viruses. of 3035 nasal/throat swabs, 494 (16.3%) samples were identified as inf ... | 2011 | 21708292 |
| detection of respiratory viruses on air filters from aircraft. | to evaluate the feasibility of identifying viruses from aircraft cabin air, we evaluated whether respiratory viruses trapped by commercial aircraft air filters can be extracted and detected using a multiplex pcr, bead-based assay. | 2011 | 21707676 |
| [efficacy of inactivating viruses by photocatalytically reacting nonwoven titanium dioxide fabric]. | titanium dioxide (tio2) photocatalysis causes oxidative destruction dependent on electrons excited by < or = 400 nm ultraviolet (uv) rays. many studies have covered the destruction of organics and bacteria and bacteriophage inactivation by photocatalysis. we studied the inactivation by new nonwoven siliconized titanium dioxide fabric of the feline calicivirus f9 (fcv-f9), human adenovirus gb (hadv3-gb), and influenza a and b virus (a/new caledonia, b/shandong, and 5 clinical strains). we spotted ... | 2011 | 21706843 |
| an influenza outbreak among pilgrims sleeping at a school without purpose built overnight accommodation facilities. | this report describes a respiratory illness outbreak amongst a group of over 700 world youth day 2008 pilgrims staying at a basic accommodation venue for 1 week in july 2008. at this venue, 1 group of pilgrims was accommodated as a large group in a gymnasium and another group was sub-divided into smaller groups and accommodated in classrooms. following confirmation of an influenza b outbreak by influenza point of care testing, control measures were promptly implemented. isolation of cases, impro ... | 2011 | 21698978 |
| overexpression of +¦-2,6 sialyltransferase stimulates propagation of human influenza viruses in vero cells. | human influenza viruses are major concern as the leading cause of global pandemics. in infecting cells, they preferentially bind to sialyloligosaccharides containing terminal n-acetyl sialic acid linked to galactose by an +¦-2,6-linkage (neuac+¦2,6gal). the amount of neuac+¦2,6gal in vero cells, which are predominantly used for production of influenza vaccines over the past 30 years, may not be as high as that in epithelial cells of human respiratory tract, what leads to the suboptimal virus gro ... | 2011 | 21692563 |
| a-ágel-capture assay for characterizing the sialyl-glycan selectivity of influenza viruses. | sialic acids (sa) usually linked to galactose (gal) in an +¦2,6- or +¦2,3-configuration are considered the main cell receptors for influenza viruses, in particular for their hemagglutinins (ha). the typing of influenza virus ha receptor selectivity is relevant for understanding the transmissibility of avian and swine viruses to the human population. in this study we developed a simple and inexpensive gel-capture assay (gca) of the influenza virus ha receptor-binding selectivity. its principle is ... | 2011 | 21692561 |
| reduction in the incidence of influenza a but not influenza b associated with use of hand sanitizer and cough hygiene in schools: a randomized controlled trial. | laboratory-based evidence is lacking regarding the efficacy of nonpharmaceutical interventions (npis) such as alcohol-based hand sanitizer and respiratory hygiene to reduce the spread of influenza. | 2011 | 21691245 |
| possible reduced effectiveness of the 2009 h1n1 component of live, attenuated influenza vaccine. | 2011 | 21690631 | |
| comparative evaluation of the seegene seeplex rv15 and real-time pcr for respiratory virus detection. | respiratory virus infections contribute substantially both to hospitalizations of young children, and to morbidity in immunocompromised patients such as those with hematological malignancies. their rapid and accurate diagnosis is essential to patient management. to evaluate the prospective utility of seeplex-« dpo technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real-time rt-pcr for one or more viruses was assayed by the seegene seeplex-« rv12 system. as w ... | 2011 | 21678451 |
| contribution of common and recently described respiratory viruses to annual hospitalizations in children in south africa. | the contribution of viruses to lower respiratory tract disease in sub-saharan africa where human immunodeficiency virus may exacerbate respiratory infections is not well defined. no data exist on some of these viruses for southern africa. comprehensive molecular screening may define the role of these viruses as single and co-infections in a population with a high hiv-aids burden. to address this, children less than 5 years of age with respiratory infections from 3 public sector hospitals, pretor ... | 2011 | 21678450 |
| pandemic and seasonal influenza viruses among patients with acute respiratory illness in kashmir (india). | please cite this paper as: koul pa., et┬áal. (2011) pandemic and seasonal influenza viruses among patients with acute respiratory illness in kashmir (india). influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00261.x. backgroundôçé with the emergence of pandemic influenza a (2009a/h1n1) virus in india, we sought to determine the prevalence and clinical presentations of seasonal and pandemic influenza viruses among acute respiratory illness (ari) patients from srinagar, a temp ... | 2011 | 21668665 |
| comparison of clinical features and outcomes of medically attended influenza a and influenza b in a defined population over four seasons: 2004-2005 through 2007-2008. | please cite this paper as: irving et┬áal. (2011) comparison of clinical features and outcomes of medically attended influenza a and influenza b in a defined population over four seasons: 2004-2005 through 2007-2008. influenza and other respiratory viruses doi: 10.1111/j.1750-2659.2011.00263.x. backgroundôçé there are few prospectively collected data comparing illnesses caused by different subtypes of influenza. we compared the clinical presentation and outcomes of subjects with primarily outpati ... | 2011 | 21668663 |
| epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. | please cite this paper as: zuccotti et┬áal. (2011) epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00263.x. backgroundôçé seasonal influenza viruses and respiratory syncytial virus (rsv) are primary causes of acute respiratory tract infections (artis) in children. new respiratory viruses including human metapneumovirus (hmpv ... | 2011 | 21668662 |
| efficient influenza b virus propagation due to deficient interferon-induced antiviral activity in mdck cells. | influenza b virus infections are mainly restricted to humans, which is partially caused by the inability of influenza b virus ns1 protein to counteract the innate immune response of other species. however, for cell culture-based influenza vaccine production non-human cells, such as madin-darby canine kidney (mdck) cells, are commonly used. therefore, the impact of cellular pathogen defence mechanisms on influenza b virus propagation in mdck cells was analysed in this study. activation of the cel ... | 2011 | 21651940 |
| viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the speed-oligo® rsv assay for the detection of respiratory syncytial virus. | respiratory syncytial virus (rsv) is the viral agent which is more frequently involved in lower respiratory tract infections (lrtis) in infants under 1 year of age in developed countries. a new oligochromatographic assay, speed-oligo® rsv, was designed and optimized for the specific detection and identification of rsv subtypes a and b. the test was evaluated in 289 clinical samples from 169 hospitalized children using an immunochromatography (ic) test, virus isolation by culture, and an in-house ... | 2011 | 21647616 |
| children with asthma hospitalized with seasonal or pandemic influenza, 2003-2009. | objective: to describe the characteristics and clinical courses of asthmatic children hospitalized with seasonal or 2009 pandemic h1n1 influenza and compare complications by influenza type. methods: during the 2003-2009 influenza seasons and the 2009 pandemic, we conducted surveillance of 5.3 million children aged 17 years or younger for hospitalization with laboratory-confirmed influenza and identified those with asthma (defined as those aged 2-17 years with a history of asthma in their medical ... | 2011 | 21646257 |
| neutralizing antibody but not hemagglutination antibody provides accurate evaluation for protective immune response to h5n1 avian influenza virus in vaccinated rabbits. | in order to develop an animal model and an assay method to evaluate protective immune response to h5n1 avian influenza vaccination, h5n1 avian influenza vaccine was prepared. new zealand rabbits were assigned to receive two doses of vaccine with different hemagglutinin (ha) dosage. the sera from vaccinated rabbits was evaluated to determine antibody titer and specificity using different tested methods including hemagglutination inhibition assay (hi), neutralizing assay (nt), cross-hi assay, cros ... | 2011 | 21645575 |
| application of a split luciferase complementation assay for the detection of viral protein-protein interactions. | intraviral protein-protein interactions are critical for virus survival in the host. discovery of such interactions is important to understand molecular mechanisms of viral replication and pathogenesis. the development of a cell-based assay that can be employed to examine systematically viral protein interactions is described. the method, known as the split luciferase complementation assay (slca), is based on the principle that n- and c-terminal domains of luciferase alone do not emit luminescen ... | 2011 | 21645548 |
| risk factors for hospitalized seasonal influenza in rural western kenya. | risk factors for influenza hospitalization in africa are unknown, including the role of hiv. | 2011 | 21637856 |