Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| enhancement of peripheral blood cd8+ t cells and classical swine fever antibodies by dietary beta-1,3/1,6-glucan supplementation in weaned piglets. | the experiment was conducted to evaluate the effect of dietary supplementation with 0, 50, 100 and 200 mg/kg of beta-1,3/1,6-glucan extracted from saccharomyces cerevisiae on cellular immune responses and classical swine fever virus (csfv) antibody production of weaned piglets. thirty-two (landrace x large white; 26 days old; 7.05+/-0.25 kg body weight) castrated male piglets were randomly allocated to four treatments, eight pigs per treatment. all piglets were vaccinated with live-attenuated ch ... | 2008 | 18761656 |
| baculovirus surface display of e(rns) envelope glycoprotein of classical swine fever virus. | classical swine fever virus (csfv) causes significant losses in the pig industry in many countries. e(rns) is an envelope glycoprotein of csfv which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. in this study, one recombinant baculoviruses bacsc-e(rns) expressing histidine-tagged e(rns) with the transmembrane domain (tm) and cytoplasmic domain (ctd) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evalu ... | 2008 | 18727937 |
| baculovirus surface display of e2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in a mouse model. | classical swine fever virus (csfv) causes significant losses in pig industry in many countries. the e2 glycoprotein of csfv is the main target for inducing neutralizing antibodies and protective immunity in the natural host. in this study, one recombinant baculoviruses bacsc-e2 expressing histidine-tagged e2 with the ctd and tm derived from baculovirus envelope protein gp64 was constructed and evaluated its vaccine efficacy in mice model. after infection, e2 was expressed and anchored on the pla ... | 2008 | 18708107 |
| antibodies to selected viral disease agents in wild boars from the czech republic. | blood samples were collected from wild boar (sus scrofa) shot during the hunting season from 1999 to 2005 in the czech republic. sera were tested by enzyme-linked immunosorbent assay for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv), and bovine viral diarrhea virus (bvdv). indirect fluorescence antibody test was used for detection of antibodies against porcine circovirus type 2 (pcv-2) and transmissible ... | 2008 | 18689671 |
| cytopathogenicity of classical swine fever virus correlates with attenuation in the natural host. | for the important livestock pathogens classical swine fever virus (csfv) and bovine viral diarrhea virus (bvdv), cytopathogenic (cp) and non-cp viruses are distinguished according to the induction of apoptosis in infected tissue culture cells. however, it is currently unknown whether cp csfv differs from non-cp csfv with regard to virulence in the acutely infected host. in this study, we generated helper virus-independent csfv alfort-jiv, which encompasses sequences encoding domain jiv-90 of cel ... | 2008 | 18653456 |
| dynamics of virus excretion via different routes in pigs experimentally infected with classical swine fever virus strains of high, moderate or low virulence. | classical swine fever virus (csfv) is transmitted via secretions and excretions of infected pigs. the efficiency and speed of the transmission depends on a multitude of parameters, like quantities of virus excreted by infected pigs. this study provides quantitative data on excretion of csfv over time from pigs infected with a highly, moderately or low virulent strain. for each strain, five individually housed pigs were infected. virus excretion was quantified in oropharyngeal fluid, saliva, nasa ... | 2009 | 18635323 |
| the npro product of classical swine fever virus interacts with ikappabalpha, the nf-kappab inhibitor. | classical swine fever virus (csfv) belongs to the genus pestivirus and is the causative agent of classical swine fever, a haemorrhagic disease of pigs. the virus replicates in host cells without activating interferon (ifn) production and has been reported to be an antagonist of double-stranded rna-induced apoptosis. the n-terminal protease (n(pro)) of csfv is responsible for this evasion of the host innate immune response. in order to identify cellular proteins that interact with the n(pro) prod ... | 2008 | 18632959 |
| electron microscope observations of african swine fever virus in tissue culture cells. | african swine fever virus, hinde isolate, grown in a stable pig kidney cell line and primary pig kidney cells, was examined for successive stages in the development of the virus particle. the mature particle has a hexagonal outer membrane structure (diameter 175-215 micron) surrounding an electron lucent region and a dense nucleoid (diameter 72-89 micron). the increase in particle production in the cell cytoplasm is consistent with both the rise in infectivity as measured in pig kidney cell cult ... | 1966 | 18611474 |
| characterization of antibody responses against a neutralizing epitope on the glycoprotein e2 of classical swine fever virus. | the sequence tavspttlr is a conserved and linear neutralizing epitope on the glycoprotein e2 of classical swine fever virus. in this study, tavspttlr-directed antibodies, induced either by virions or by an epitope-focused immunogen, were characterized. the results revealed that despite the same epitope specificity, the antibodies induced by different immunogens varied significantly both in the neutralizing test and in binding inhibition assays. this suggests that the protective immunity induced ... | 2008 | 18607674 |
| survival of classical swine fever virus at various temperatures in faeces and urine derived from experimentally infected pigs. | indirect transmission of classical swine fever virus (csfv) can occur through contact with mechanical vectors, like clothing and footwear or transport vehicles, contaminated with the secretions or excretions of infected pigs. a prerequisite for indirect transmission is survival of the virus on the mechanical vector. consequently, to obtain more insight into these transmission routes, it is important to know how long the virus remains viable outside the host. in this study we examined the surviva ... | 2008 | 18602226 |
| rapid detection and differentiation of wild-type and three attenuated lapinized vaccine strains of classical swine fever virus by reverse transcription polymerase chain reaction. | a simple one-step reverse transcription polymerase chain reaction (rt-pcr) method was developed based on t-rich insertions in the viral genome for simultaneous detection and differentiation of wild type and vaccine strains of classical swine fever virus (csfv). the csfv-specific primers were designed to contain the sequences of the t-rich insertion sites that exist uniquely in the 3' nontranslated regions (3' ntr) of the genome of lapinized csfv vaccine strains. by using a one-step rt-pcr or a n ... | 2008 | 18599849 |
| altered order of substrate binding by dna polymerase x from african swine fever virus. | a sequential ordered substrate binding established previously for several dna polymerases is generally extended to all dna polymerases, and the characterization of novel polymerases is often based on the assumption that the enzymes should productively bind dna substrate first, followed by template-directed dntp binding. the comprehensive kinetic study of dna polymerase x (pol x) from african swine fever virus reported here is the first analysis of the substrate binding order performed for a low- ... | 2008 | 18598057 |
| simultaneous detection of classical swine fever virus and north american genotype porcine reproductive and respiratory syndrome virus using a duplex real-time rt-pcr. | classical swine fever and porcine reproductive and respiratory syndrome are both notifiable diseases of the world organization for animal health (oie). the two diseases exhibit indistinguishable clinical symptoms and sometimes co-exist in swine herds. in this study, a duplex real-time rt-pcr for simultaneous detection of classical swine fever virus (csfv) and north american (na) genotype porcine reproductive and respiratory syndrome virus (prrsv) based on two differently labeled taqman probes wa ... | 2008 | 18582953 |
| [persistent infection with the classical swine fever virus in vaccinated animals: a risk factor?]. | 2008 | 18578143 | |
| two outbreaks of classical swine fever in wild boar in france. | in 2002 and 2003, two successive outbreaks of classical swine fever were declared in wild boar in northern france. the first was in moselle, near the town of thionville and the border with luxembourg, and the second was in the northern vosges area, near the german border. the outbreaks were investigated by serological and virological diagnosis of dead or shot animals. hunting restrictions were applied to limit the spread of the outbreaks. the virus was detected eight times between april and july ... | 2008 | 18567928 |
| the envelope of intracellular african swine fever virus is composed of a single lipid bilayer. | african swine fever virus (asfv) is a member of a family of large nucleocytoplasmic dna viruses that include poxviruses, iridoviruses, and phycodnaviruses. previous ultrastructural studies of asfv using chemical fixation and cryosectioning for electron microscopy (em) have produced uncertainty over whether the inner viral envelope is composed of a single or double lipid bilayer. in this study we prepared asfv-infected cells for em using chemical fixation, cryosectioning, and high-pressure freezi ... | 2008 | 18550658 |
| retrospective analysis of the oral immunisation of wild boar populations against classical swine fever virus (csfv) in region eifel of rhineland-palatinate. | in the present study the effect of control measures implemented during the classical swine fever (csf) epidemic in wild boar in the eifel region of the german federal state of rhineland-palatinate from 1999 to 2005 was assessed. during the first 3 years after official confirmation of virus detection these measures comprised intensive hunting, especially of young animals and hygiene measures. subsequently oral immunisation (o.i.) using a modified live virus vaccine was introduced as an additional ... | 2008 | 18534790 |
| development of a magnetic bead microarray for simultaneous and simple detection of four pestiviruses. | this study reports a novel method for the rapid detection and identification of the four recognized species in the pestivirus genus of the flaviviridae family, i.e. classical swine fever virus (csfv), border disease virus (bdv), bovine viral diarrhoea virus type 1 (bvdv1) and type 2 (bvdv2). the analysis of pestivirus pcr products was performed on microarrays by means of magnetic bead detection. the process utilizes an oligonucleotide array, onto which 5' biotinylated pcr products were hybridize ... | 2009 | 18514335 |
| diagnostic specificity of a real-time rt-pcr in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection. | foot-and-mouth disease virus (fmdv) and classical swine fever virus (csfv) are highly contagious and can cause great economic losses when introduced into disease-free regions. accurate estimates of diagnostic specificity (sp) are important when considering the implementation of surveillance for these agents. the purpose of this study was to estimate diagnostic sp of a real-time reverse-transcriptase pcr assay developed for detection of fmdv in cattle and domestic swine and csfv in domestic swine ... | 2008 | 18499360 |
| general preadaptation of viral infectors to their hosts. | we tested the hypothesis of optimal adaptation of viral infectors to eukaryotic hosts, using (1) correlation in codon and amino acid usage between organisms, and (2) canonical correlation between groups of hosts and infectors. the codon correlations between parasites and hosts vary, being low between swine and african swine fever virus (asf; r = 0.18), and highest between potato and potato virus x (r = 0.60). the correlations might indicate different stages of evolution toward optimal adaptation ... | 2008 | 18493153 |
| identification of a conserved linear b-cell epitope at the n-terminus of the e2 glycoprotein of classical swine fever virus by phage-displayed random peptide library. | the e2 protein of classical swine fever virus (csfv) is an important envelope glycoprotein, which is responsible for inducing protective immune response in swine. four antigenic domains, a-d, have been mapped on the e2 protein. the present study describes the identification of a linear b-cell epitope at the n-terminus of the e2 protein by screening a phage-displayed random 12-peptide library with the neutralizing monoclonal antibody (mab) hq06 directed against the e2 protein. hq06 was found to b ... | 2008 | 18485511 |
| the gtp binding sites interacted with rna-dependent rna polymerase of classical swine fever virus in de novo initiation. | the ns5b protein of classical swine fever virus (csfv) is an important enzyme bearing a unique rna-dependent rna polymerase (rdrp) activity. the rdrp plays a crucial role in the viral replication cycle and in forming a replicase complex. however, the initiating synthesis mechanism of the csfv rna polymerase is unclearly described at present. our aim is to reveal the rdrp-gtp docking sites and the effective modules of gtp initially bound to the polymerase in starting initiation of replication acc ... | 2008 | 18430987 |
| complete genome sequence of attenuated low-temperature thiverval strain of classical swine fever virus. | the thiverval vaccine strain of classical swine fever virus (csfv) was derived from virulent alfort strain through the serial passages in cells at 29-30 degrees c. in this study, we determined the complete genome sequence of this strain and found that its genome contains one open reading frame (orf) that encodes a polyprotein with 3,898 amino acids. the 5'-utr of thiverval is 373 nt long with only one mutation at position 220. in contrast, the length of 3'-utr is highly heterogeneous ranging fro ... | 2008 | 18401695 |
| cytokine mrna expression and pathological findings in pigs inoculated with african swine fever virus (e-70) deleted on a238l. | african swine fever virus (asfv) induces a variety of immune responses and clinical forms in domestic pigs. as it is the only member of the asfarviridae family, asfv encodes many novel genes not encoded by other virus families. among these genes, a238l may regulate the synthesis of pro-inflammatory cytokines, controlled mainly by nfkappab and nfat pathways. in this study, we inoculated two groups of pigs, one with the asfv highly virulent e-70 isolate, deleted on a238l gene, and the other group ... | 2008 | 18384883 |
| role of hepatic macrophages during the viral haemorrhagic fever induced by african swine fever virus. | to ascertain the role played by the various liver monocyte-macrophage populations in the course of a viral hemorrhagic fever, fifteen pigs were inoculated intramuscularly with the highly virulent isolate of african swine fever virus (asfv) españa-70 and slaughtered at 1-7 days post-inoculation (dpi). samples of liver were fixed in different solutions and routinely processed for morphological, immunohistochemical and ultrastructural studies. viral antigen (vp73) was detected from 3 dpi onward, ma ... | 2008 | 18366006 |
| effect of ns3 and ns5b proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation. | a full-length ns3 (ns3f) and a truncated ns3 protein (ns3h) with an rna helicase domain possess rna helicase activity. using an in vitro system with a monocistronic reporter rna or dna, containing the csfv 5'-utr, we observed that both ns3f and ns3h enhanced internal ribosome entry site (ires)-mediated and cellular translation in a dose-dependent manner, but ns3 protease (ns3p) that lacks a helicase domain did not. ns3f was stronger than ns3h in promoting both translations. these results showed ... | 2008 | 18343841 |
| quantitative assessment of the likelihood of the introduction of classical swine fever virus into the danish swine population. | classical swine fever virus (csfv) is a major infectious-disease agent of livestock and causes production losses through increased morbidity and mortality, particularly of young pigs. we identified the pathways for introduction of csfv into denmark and assessed the annual probability of introduction (based on a us department of agriculture model). we developed pathways based on material from scientific articles, reports from veterinary agencies and custom officers, and consultations with experts ... | 2008 | 18342380 |
| simulating the spread of classical swine fever virus between a hypothetical wild-boar population and domestic pig herds in denmark. | denmark has no free-range wild-boar population. however, danish wildlife organizations have suggested that wild boar should be reintroduced into the wild to broaden national biodiversity. danish pig farmers fear that this would lead to a higher risk of introduction of classical swine fever virus (csfv), which could have enormous consequences in terms of loss of pork exports. we conducted a risk assessment to address the additional risk of introducing and spreading csfv due to the reintroduction ... | 2008 | 18339438 |
| eif2-dependent and eif2-independent modes of initiation on the csfv ires: a common role of domain ii. | specific interactions of the classical swine fever virus internal ribosomal entry site (ires) with 40s ribosomal subunits and eukaryotic translation initiation factor (eif)3 enable 43s preinitiation complexes containing eif3 and eif2-gtp-met-trna(imet) to bind directly to the initiation codon, yielding 48s initiation complexes. we report that eif5b or eif5b/eif3 also promote met-trna(imet) binding to ires-40s complexes, forming 48s complexes that can assemble elongation-competent ribosomes. alth ... | 2008 | 18337746 |
| a179l, a viral bcl-2 homologue, targets the core bcl-2 apoptotic machinery and its upstream bh3 activators with selective binding restrictions for bid and noxa. | several large dna viruses encode bcl-2 protein homologues involved in the regulation of the cellular apoptosis cascade. this regulation often involves the interaction of these viral proteins with diverse cellular bcl-2 family members. we have identified the specific interactions of a179l, an african swine fever virus (asfv) bcl-2 homologue, with the active forms of the porcine bh3-only bid protein (truncated bid p13 and p15). transient expression of asfv a179l gene in vero cells prevented apopto ... | 2008 | 18329683 |
| classical swine fever virus strain "c" protects the offspring by oral immunisation of pregnant sows. | the aim of this study was to evaluate if oral immunisation of wild sows protects the fetuses from transplacental infection. two experiments were carried out with gilts vaccinated orally with c-strain virus approximately 5 weeks after insemination. they were challenged at mid-gestation with highly virulent classical swine fever virus (csfv) or moderately virulent field virus. the results revealed that oral vaccination has no negative impact on the pregnancy, and all vaccinated sows developed neut ... | 2008 | 18321665 |
| [construction of recombinant fowlpox virus expressing e0 gene of classical swine fever virus shimen strain and the animal immunity experiment]. | the csfv e0 gene was amplified from the plasmid pmd18-t-e0 by pcr and cloned into the fpv-p11 and fpv-psy. the identified recombinant dna was transfected into chicken embryo fibroblasts (cef) to package fowlpox virus. e0 gene was confirmed to be integrated into the genome of recombinant fowlpox virus by pcr, and western blot was employed for detection of e0 expression in the chicken embryo fibroblasts infected with recombinant fowlpox virus . the results of elisa showed that systemic immune resp ... | 2008 | 18320824 |
| local spread of classical swine fever upon virus introduction into the netherlands: mapping of areas at high risk. | in the recent past, the introduction of classical swine fever virus (csfv) followed by between-herd spread has given rise to a number of large epidemics in the netherlands and belgium. both these countries are pork-exporting countries. particularly important in these epidemics has been the occurrence of substantial "neighborhood transmission" from herd to herd in the presence of base-line control measures prescribed by eu legislation. here we propose a calculation procedure to map out "high-risk ... | 2008 | 18298803 |
| the expression of classical swine fever virus structural protein e2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors. | it has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown to induce protection antibodies. the structural protein e2 of classical swine fever virus (csfv), which has been shown to carry critical epitopes, has been expressed in different systems. here, we report the expression of cfsv e2 gene in tobacco chloroplasts. mice immunized with leaf extracts elicited specific antibodies. this indicated that the expressed e2 proteins had a ... | 2008 | 18280983 |
| promotion of immunity of mice to pasteurella multocida and hog cholera vaccine by pig interleukin-6 gene and cpg motifs. | a novel oligodeoxynuleotides containing 11 cpg motifs was synthesized and inserted into the vr1020 plasmid containing pig interleukin-6 (il-6) gene (vpil6) to construct recombinant plasmid, vpil6c. the chitosan nanoparticles (cnp) were prepared by ionic cross linkage to entrap the vpil6c (vpil6c-cnp), vpil6 (vpil6-cnp) and cpg (cpg-cnp). 42-day old female mice were divided into four groups and intramuscularly injected respectively with 6 pmol vpil6c-cnp, vpil6-cnp, cpg-cnp and vr1020-cnp along w ... | 2009 | 18279956 |
| 12-nt insertion in 3' untranslated region leads to attenuation of classic swine fever virus and protects host against lethal challenge. | we report here the discovery of an attenuation mechanism of classic swine fever virus (csfv) induced by introduction of a continuous 12-nt (cuuuuuucuuuu) insertion in viral 3' utr. the 12-nt insertion sequence was first found in one attenuated vaccine strain hclv (hog cholera lapinized virus) which did not exist in other csfv strains. to address the function of the 12-nt insertion in viral replication and attenuation, we constructed and analyzed two chimeras stemmed from a highly virulent strain ... | 2008 | 18279903 |
| in vitro inhibition of classical swine fever virus replication by sirnas targeting npro and ns5b genes. | classical swine fever (csf) is a highly contagious disease of pigs, which causes important economic losses worldwide. in the present study, the specific effect of rna interference on the replication of csf virus (csfv) was explored. three species of small interfering rna (sirna), targeting different regions of csfv npro and ns5b genes, were prepared by in vitro transcription. after transfection of pk-15 cells with each of the sirnas followed by infection with csfv, the viral proliferation within ... | 2008 | 18262291 |
| development of a reliable assay protocol for identification of diseases (rapid)-bioactive amplification with probing (bap) for detection of newcastle disease virus. | due to appearance of new genotypes of newcastle disease virus (ndv) with no cross-protection and with vaccine strains, some outbreaks have been reported in taiwan that caused significant damage to the poultry industry. a reliable assay protocol, (rapid)-bioactive amplification with probing (bap), for detection of ndv that uses a nested pcr and magnetic bead-based probe to increase sensitivity and specificity, was developed. primers and probes were designed based on the conserved region of the f ... | 2008 | 18261864 |
| domains involved in calcineurin phosphatase inhibition and nuclear localisation in the african swine fever virus a238l protein. | the african swine fever virus a238l protein inhibits calcineurin phosphatase activity and activation of nf-kappab and p300 co-activator. an 82 amino acid domain containing residues 157 to 238 at the c-terminus of a238l was expressed in e. coli and purified. this purified a238l fragment acted as a potent inhibitor of calcineurin phosphatase in vitro with an ic50 of approximately 70 nm. two putative nuclear localisation signals were identified between residues 80 to 86 (nls-1) and between residues ... | 2008 | 18261759 |
| african swine fever virus dna in soft ticks, senegal. | african swine fever is a highly contagious disease of pigs in africa. although its persistence in senegal may be caused by asymptomatic carriers involved in the domestic transmission cycle, we demonstrated that the soft tick ornithodoros sonrai can be naturally infected with the causative agent. | 2007 | 18258050 |
| african swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection. | african swine fever virus (asfv), a large enveloped dna-containing virus, infects domestic and wild pigs, and multiplies in soft ticks, causing an economically relevant hemorrhagic disease. evaluation of the nuclear import ability of asfv p10 protein was the major purpose of the present work. two approaches were used to determine if p10 protein is imported into the nucleus by an active process: a yeast-based nuclear import assay and the determination of the subcellular localization of p10 protei ... | 2008 | 18243588 |
| efficacy of intradermally administrated e2 subunit vaccines in reducing horizontal transmission of classical swine fever virus. | to investigate if intradermal (id) vaccination and intramuscular (im) vaccination result in a comparable reduction of horizontal transmission of classical swine fever virus (csfv), two registered e2 subunit marker vaccines were examined. vaccine a was a water-in-oil emulsion containing the e2 glycoprotein originating from the alfort/tübingen strain and vaccine b was a water-oil-water emulsion containing the e2 glycoprotein originating from the brescia strain. eight groups, of ten pigs each, were ... | 2008 | 18242794 |
| cytopathic effect of classical swine fever virus ns3 protein on pk-15 cells. | in order to further research the relationship between classical swine fever virus' (csfv) ns3 protein and the cytopathic effect (cpe) in cells infected with the csfv, and to reveal the effect of protein ns3 on the host cells, the ns3 of csfv shimen strain amplified by rt-pcr was subcloned into the pegfp-c1, named pegfp-c1-ns3. the insert position, the size and the reading frame were correct for restriction enzyme digestion and sequence analysis. the pegfp-c1-ns3 and pegfp-c1 were transfected int ... | 2008 | 18204288 |
| comparison of the genome sequences of non-pathogenic and pathogenic african swine fever virus isolates. | the genomic coding sequences, apart from the inverted terminal repeats and cross-links, have been determined for two african swine fever virus (asfv) isolates from the same virus genotype, a non-pathogenic isolate from portugal, ourt88/3, and a highly pathogenic isolate from west africa, benin 97/1. these genome sequences were annotated and compared with that of a tissue culture-adapted isolate, ba71v. the genomes range in length between 170 and 182 kbp and encode between 151 and 157 open readin ... | 2008 | 18198370 |
| classical swine fever virus e2 glycoprotein antigen produced in adenovirally transduced pk-15 cells confers complete protection in pigs upon viral challenge. | e2 is the major envelope glycoprotein present on the outer surface of the classical swine fever virus (csfv). it is exposed as a homodimer originated by disulfide linkage and represents an important target for the induction of neutralizing immune responses against the viral infection. the e2his glycoprotein nucleotide sequence used in this work contains the csfv e2 extracellular domain preceded by the tissue plasminogen signal peptide and a hexa-histidine tag in the 3' terminus. the recombinant ... | 2008 | 18192093 |
| feed contaminated with classical swine fever vaccine virus (lom strain) can induce antibodies to the virus in pigs. | in november 2004, antibodies to classical swine fever virus (csfv) were detected in finishing pigs during the annual serological surveillance in jeju province, korea. in addition, csf vaccine viruses (lom strain) had recently been isolated from pigs raised on farms known to have csfv antibody-positive pigs. in contrast with mainland korea, jeju province had been csf free and its pigs had not been vaccinated against csf for more than five years. an epidemiological investigation team from the nati ... | 2008 | 18178932 |
| effect of n-glycosylation inhibition on the synthesis and processing of classical swine fever virus glycoproteins. | classical swine fever virus (csfv) is often used as a surrogate model in molecular studies of the closely related hepatitis c virus. in this report we have examined the effect of the inhibition of glycosylation on the survival and maturation of csfv. viral glycoproteins (e(rns), e1, e2) form biologically active complexes - homo- and heterodimers, which are indispensable for viral life cycle. those complexes are highly n-glycosylated. we studied the influence of n-glycosylation on dimer formation ... | 2007 | 18084653 |
| solution structures of 2 : 1 and 1 : 1 dna polymerase-dna complexes probed by ultracentrifugation and small-angle x-ray scattering. | we report small-angle x-ray scattering (saxs) and sedimentation velocity (sv) studies on the enzyme-dna complexes of rat dna polymerase beta (pol beta) and african swine fever virus dna polymerase x (asfv pol x) with one-nucleotide gapped dna. the results indicated formation of a 2 : 1 pol beta-dna complex, whereas only 1 : 1 pol x-dna complex was observed. three-dimensional structural models for the 2 : 1 pol beta-dna and 1 : 1 pol x-dna complexes were generated from the saxs experimental data ... | 2008 | 18084022 |
| efficacy of lyophilised c-strain vaccine after oral immunisation of domestic pigs and wild boar against classical swine fever: first results. | the aim of this study was to evaluate the efficacy of lyophilised c-strain vaccine in domestic pigs and wild boar after oral application. a new spherical bait form (diameter 3 cm) containing lyophilised vaccine virus and the recent vaccine baits were used for animal experiments. four vaccination groups were established in experiment 1 (group 1: recent liquid bait vaccine; group 2: spherical baits containing one dose of the lyophilised vaccine; groups 3 (domestic pigs) and 4 (wild boar): spherica ... | 2007 | 18077931 |
| inhibition of expression of rna polymerase with small interfering rnas targeting a conserved motif in the respective viral genes in viruses of the family flaviviridae. | the rna-dependent rna polymerase (rdrp) in viruses of the family flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. we used small interfering rnas (sirnas) corresponding to the highly conservative motif v of rdrp gene of different viruses to examine their role in modulating the expression of rdrp. evaluation of the expression of rdrps was performed by the fluorescence, flow cytometry, western blotting, and real-time pcr. we found that ... | 2007 | 18076310 |
| classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs. | classical swine fever virus (csfv) compromises the host immune system, causing indirect leucopoenia and disruption of in vitro t cell stimulation capacity. in order to explore the potential role of dendritic cells (dc) in such phenomena, the activation of conventional dc (cdc) and plasmacytoid dc (pdc) in blood and secondary lymphoid organs of infected pigs was investigated in the early time course post-inoculation (pi), together with viral components dissemination and cytokine production in ser ... | 2008 | 18073094 |
| [replicative kinetics of classical swine fever virus in pk-15 cells]. | in order to understand the replication kinetics of classical swine fever virus (csfv) in in vitro cells pk-15 cells were seeded in 96-well tissues culture plates. after overnight incubation at 37 degrees c in 5% co2 environment when growing to 80% confluence, the cells were infected with csfv strain shimen at 100 tcid50 per well. at various time post infection (p.i.) the replication of the virus in the cells were analyzed repectively by detection of viral antigen using indirect immunofluorescent ... | 2007 | 18062252 |
| descriptive summary of an outbreak of porcine post-weaning multisystemic wasting syndrome (pmws ) in new zealand. | investigations were conducted to determine the cause of an acute, multi-farm outbreak of porcine respiratory disease that included diarrhoea and subsequent loss of body condition in affected pigs. a definition for post-weaning multisystemic wasting syndrome (pmws) including both clinical and pathological features, previously developed for the pig industry in new zealand, was applied to the current outbreak. in addition to self-reporting by owners of affected farms, local veterinarians, disease a ... | 2007 | 18059655 |
| [the oral immune efficacy of recombinant lactobacillus casei expressing csfv e290 peptide and it elicited specific ctl response]. | the gene encoding classical swine fever virus (csfv) t cell epitope e290 peptide was synthesized by pcr, cloned into the expression vector ppg-vp2 and named ppg-vp2-e290. the recombinant plasmid was electrotransformed into lactobacillus casei 393 generating ppg-vp2-e290/l. casei 393. specific anti-csfv e290 peptide immunoglobulin g (igg) antibody was detected by indirect elisa in the serum of balb/c mice and rabbits immunized with recombinant strain by oral administration. the ctl of e290 was an ... | 2007 | 18051877 |
| highly protective e2-csfv vaccine candidate produced in the mammary gland of adenoviral transduced goats. | classical swine fever virus is the etiological agent of the most economically important highly contagious disease of swine worldwide. e2 is the major envelope glycoprotein present as a homodimer on the outer surface of the virus and represents an important target for the induction of neutralizing immune response against the viral infection. the e2 extracellular domain was expressed in the milk of adenoviral transduced goats at the highest level about 1.2g/l. the recombinant glycoprotein was puri ... | 2008 | 18045719 |
| the internal initiation of translation in bovine viral diarrhea virus rna depends on the presence of an rna pseudoknot upstream of the initiation codon. | bovine viral diarrhea virus (bvdv) is the prototype representative of the pestivirus genus in the flaviviridae family. it has been shown that the initiation of translation of bvdv rna occurs by an internal ribosome entry mechanism mediated by the 5' untranslated region of the viral rna 1. the 5' and 3' boundaries of the ires of the cytopathic bvdv nadl have been mapped and it has been suggested that the ires extends into the coding of the bvdv polyprotein 2. a putative pseudoknot structure has b ... | 2007 | 18034871 |
| n(pro) fusion technology to produce proteins with authentic n termini in e. coli. | we describe a prokaryotic expression system using the autoproteolytic function of n(pro) from classical swine fever virus. proteins or peptides expressed as n(pro) fusions are deposited as inclusion bodies. on in vitro refolding by switching from chaotropic to kosmotropic conditions, the fusion partner is released from the c-terminal end of the autoprotease by self-cleavage, leaving the target protein with an authentic n terminus. a tailor-made n(pro) mutant called eddie, with increased in vitro ... | 2007 | 18026112 |
| real-time rt-pcr assay for rapid and specific detection of classical swine fever virus: comparison of sybr green and taqman mgb detection methods using novel mgb probes. | real-time pcr is an accurate, rapid and reliable method that can be used for the detection and also for the quantitation of specific dna molecules. the basic principle is the recurring measurement of a fluorescent signal, which is proportional to the amount of amplification product. in our trial two detection systems were tested for classical swine fever virus (csfv) detection and for its discrimination from other pestiviruses; non-specific dsdna-binding dye sybr green and specific fluorogenic t ... | 2008 | 18001851 |
| value of skin punch biopsies for the diagnosis of acute classical swine fever. | the objective of this study was to determine if skin punch biopsies are appropriate for the diagnosis of classical swine fever. for this purpose, 6 wild boars and 2 domestic pigs were experimentally infected with the highly virulent classical swine fever virus (csfv) koslov and 5 domestic pigs with a csfv field isolate (genotype 2.3 uelzen) derived from wild boar. skin biopsy specimens were tested using virus isolation, real-time reverse transcription polymerase chain reaction (rtrt-pcr), and fl ... | 2007 | 17998561 |
| imidazo[4,5-c]pyridines inhibit the in vitro replication of the classical swine fever virus and target the viral polymerase. | selective inhibitors of the replication of the classical swine fever virus (csfv) may have the potential to control the spread of the infection in an epidemic situation. we here report that 5-[(4-bromophenyl)methyl]-2-phenyl-5h-imidazo[4,5-c]pyridine (bpip) is a highly potent inhibitor of the in vitro replication of csfv. the compound resulted in a dose-dependent antiviral effect in pk(15) cells with a 50% effective concentration (ec(50)) for the inhibition of csfv alfort(187) (subgroup 1.1) of ... | 2008 | 17997169 |
| genetic diversity of the envelope glycoprotein e2 of classical swine fever virus: recent isolates branched away from historical and vaccine strains. | the envelope glycoprotein e2 of classical swine fever virus (csfv) plays multiple roles in the viral life cycle, interaction with host and pathogenesis. we sequenced the e2 gene of 34 csfv isolates from southeastern china for analysis of genetic diversity in this particular region. phylogenetic analysis revealed that genotype 2.1b viruses became predominant in southeastern china with 33 isolates clustered in 2.1b and only 1 isolate belonged to 2.2. pairwise comparisons demonstrated isolates in t ... | 2008 | 17976931 |
| development of a novel hot-start multiplex pcr for simultaneous detection of classical swine fever virus, african swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus. | a novel hot-start multiplex pcr (mpcr) assay was developed and subsequently evaluated for its effectiveness in simultaneously detecting multiple viral infections of swine. specific primers for each of five virus genomes, namely classical swine fever virus (csfv), african swine fever virus (asfv), porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv) and porcine parvovirus (ppv) were used. combined nucleic acid purification was carried out using a commercia ... | 2008 | 17975735 |
| [differences in glycosylation of the e2 protein between virulent shimen strain and a virulent c-strain of classical swine fever virus]. | the e2 envelope glycoprotein of virulent shimen strain and avirulent c-strain of classical swine fever virus (csfv) has 5 and 6 potential glycosylation sites, respectively, and the potential glycosylation site 986n is unique to c-strain. to study the differences in glycosylation between the virus pair, the e2 genes (removing signal sequence and transmembrane anchor regions) of the two strains fused with the melittin signal sequence were expressed in the sf9 insect cells. the recombinant e2 prote ... | 2007 | 17969857 |
| classical swine fever virus inhibits nitric oxide production in infected macrophages. | classical swine fever virus (csfv)-macrophage interactions during infection were analysed by examining macrophage transcriptional responses via microarray. eleven genes had increased mrna levels (>2.5-fold, p<0.05) in infected cell cultures, including arginase-1, an inhibitor of nitric oxide production, phosphoinositide 3-kinase, chemokine receptor 4 and interleukin-1beta. lower levels of nitric oxide and increased arginase activity were found in csfv-infected macrophages. these changes in gene ... | 2007 | 17947523 |
| the npro product of classical swine fever virus and bovine viral diarrhea virus uses a conserved mechanism to target interferon regulatory factor-3. | classical swine fever virus (csfv) is a member of the genus pestivirus in the family flaviviridae. the n(pro) product of csfv targets the host's innate immune response and can prevent the production of type i interferon (ifn). the mechanism by which csfv orchestrates this inhibition was investigated and it is shown that, like the related pestivirus bovine viral diarrhea virus (bvdv), this involves the n(pro) protein targeting interferon regulatory factor-3 (irf-3) for degradation by proteasomes ... | 2007 | 17947522 |
| [co-expression of csfv t cell epitope e290 peptide and ppv vp2 protein in lactobacillus casei and determination of specific antibodies in immunized mice]. | lactobacillus casei strain 393 was selected as an antigen delivery vehicle for the development of oral vaccine to express recombinant classical swine fever virus (csfv) t cell epitope e290 peptide and porcine parvovirus (ppv) vp2 protein. the recombinant genes encoding csfv t cell epitope e290 peptide and ppv vp2 protein, respectively, were cloned into the secretion expression vector ppg, and then the ppg-vp2-e290 was electrotransformed into l. casei 393 giving rise to recombinant strain ppg-vp2 ... | 2007 | 17944369 |
| interactions of the dna polymerase x from african swine fever virus with gapped dna substrates. quantitative analysis of functional structures of the formed complexes. | energetics and specificity of interactions between the african swine fever virus polymerase x and gapped dna substrates have been studied, using the quantitative fluorescence titration technique. stoichiometries of pol x complexes, with the dna substrates, are higher than suggested by nmr studies. this can be understood in the context of the functionally heterogeneous organization of the total dna-binding site of pol x, which is composed of two dna-binding subsites. the enzyme forms two differen ... | 2007 | 17941646 |
| diagnosis of classical swine fever virus in a limited resource setting: the influence of pig breed on methodology and sample selection. | 2007 | 17941484 | |
| immunity against ns3 protein of classical swine fever virus does not protect against lethal challenge infection. | classical swine fever is a highly contagious disease of swine caused by classical swine fever virus, an oie list a pathogen. in the european union the virus has been eradicated from the domestic pig population and prophylactic immunization has been banned. nevertheless, intervention immunizations using marker vaccines are one possibility to deal with reintroduced csfv. at present, baculovirus-expressed e2 protein is used as such a marker vaccine. however, this vaccine cannot fully protect agains ... | 2007 | 17931119 |
| the selection pressure analysis of classical swine fever virus envelope protein genes erns and e2. | classical swine fever virus, one member of the family flaviviridae, is the pathogen of csf, an economically important and highly contagious disease of pigs. knowledge of virus genes under positive selection pressure can help identify molecular determinants of virulence or pathogenesis without prior knowledge of the mechanisms governing virulence and pathogenesis and clarify the driving force of classical swine fever virus evolution. the positive selection pressure acting on envelope protein gene ... | 2008 | 17928086 |
| a novel role of classical swine fever virus e(rns) glycoprotein in counteracting the newcastle disease virus (ndv)-mediated ifn-beta induction. | e(rns) is an envelope glycoprotein of classical swine fever virus (csfv) and has an unusual feature of rnase activity. in the present study, we demonstrate that e(rns) counteracts newcastle disease virus (ndv)-mediated induction of ifn-beta. for this purpose, e(rns) fused to the enhanced green fluorescent protein (egfp) was transiently expressed in porcine kidney 15 (pk15) cells. in luciferase activity assay, e(rns)-egfp was found to prevent ifn-beta promoter-driven luciferase expression and blo ... | 2007 | 17927891 |
| validation of two commercial real-time rt-pcr kits for rapid and specific diagnosis of classical swine fever virus. | two real-time rt-pcr kits, developed by lsi (taqvet csf) and adiagene (adiavet csf), obtained an agreement to be commercialised in france, subject to conditions, defined by the french classical swine fever (csf) national reference laboratory. the producers were asked to introduce an internal control to check the rna extraction efficacy. the different criteria assessed were sensitivity, "pestivirus specificity", reproducibility and ease of handling, using 189 different samples. these samples were ... | 2008 | 17913249 |
| removal of a n-linked glycosylation site of classical swine fever virus strain brescia erns glycoprotein affects virulence in swine. | e(rns) glycoprotein, along with e(1) and e(2), is one of the three envelope glycoproteins of classical swine fever virus (csfv). e(rns) is a heavily glycosylated protein involved in several functions, including virus attachment and entry to target cells, production of neutralizing antibodies, and virulence. the role of added glycans to csfv strain brescia e(rns) on virus virulence was assessed in swine. a panel of virus mutants was constructed and used to investigate whether the removal of each ... | 2008 | 17904607 |
| detection and quantification of classical swine fever virus in air samples originating from infected pigs and experimentally produced aerosols. | during epidemics of classical swine fever (csf), neighbourhood infections occurred where none of the 'traditional' routes of transmission like direct animal contact, swill feeding, transport contact or transmission by people could be identified. a hypothesized route of virus introduction for these herds was airborne transmission. in order to better understand this possible transmission route, we developed a method to detect and quantify classical swine fever virus (csfv) in air samples using gel ... | 2008 | 17869455 |
| recent advances in the development of recombinant vaccines against classical swine fever virus: cellular responses also play a role in protection. | classical swine fever virus (csfv) is the causative agent of one of the most devastating porcine haemorrhagic viral diseases, classical swine fever (csf). csfv mainly infects endothelial cells and macrophages and at the same time promotes bystander apoptosis of the surrounding t cells, causing strong immune suppression and high mortality rates. most animals experience acute infection, during which they either die or survive by producing neutralising antibodies to the virus. however, in a few cas ... | 2008 | 17804267 |
| interactions of the dna polymerase x of african swine fever virus with double-stranded dna. functional structure of the complex. | interactions of the polymerase x of african swine fever virus with the double-stranded dna (dsdna) have been studied with fluorescent dsdna oligomers, using quantitative fluorescence titrations, analytical ultracentrifugation, and fluorescence energy transfer techniques. studies with unmodified dsdnas were performed, using competition titration method. asv pol x binds the dsdna with a site-size of n=10(+/-2) base-pairs, which is significantly shorter than the total site-size of 16(+/-2) nucleoti ... | 2007 | 17765921 |
| antigenic differentiation of classical swine fever vaccinal strain pav-250 from other strains, including field strains from mexico. | twenty-nine classical swine fever virus (csfv) strains were grown in the pk15 or sk6 cell lines. antigenic differentiation studies were performed using monoclonal antibodies (mcabs), produced at lelystad (cdi-dlo), the netherlands. the monoclonals which were classified numerically as monoclonals 2-13. epitope map patterns that resulted from the reactivity with mcabs were found to be unrelated to the pathogenicity of the viruses studied. antigenic determinants were recognized by mcabs 5 and 8, we ... | 2007 | 17728020 |
| complete sequence of a subgroup 3.4 strain of classical swine fever virus from taiwan. | classical swine fever viruses from taiwan have been classified into two subgroups (3.4 and 2.1). outbreaks caused by 3.4 viruses were reported in taiwan prior to 1996 and which mainly distributed in the geographic range from southern japan to taiwan. we have determined the complete sequence of a reference strain, 94.4/il/94/twn. the genome contains 12,296 nucleotides, encoding 3,898 amino acids flanked by a 372-nt region at the 5' untranslated region (utr) and a 227-nt region at the 3'-utr. simi ... | 2007 | 17721814 |
| numerical taxonomy of the genus pestivirus based on palindromic nucleotide substitutions in the 5' untranslated region. | the palindromic nucleotide substitutions (pns) at the three variable loci (v1, v2 and v3) in the 5' untranslated region (utr) of pestivirus rna have been considered for taxonomical segregation of species, through the evaluation of 430 genomic sequences. on the basis of qualitative and quantitative secondary structure characteristics, six species have been identified: bovine viral diarrhea virus 1 (bvdv-1), bovine viral diarrhea virus 2 (bvdv-2), classical swine fever virus (csfv), border disease ... | 2007 | 17719098 |
| systematic analysis of longitudinal serological responses of pigs infected experimentally with african swine fever virus. | the protective immune response to african swine fever virus (asfv) includes both cellular and serological components. in this study, the role of antibodies in the pathogenicity and diagnosis of african swine fever (asf) was explored. accordingly, total and ig isotype antibody responses against the 12 viral proteins previously demonstrated to be the main targets of serological immunity were evaluated in longitudinally collected sera from pigs infected experimentally with the non-pathogenic asfv/n ... | 2007 | 17698651 |
| intra-genotypic resolution of african swine fever viruses from an east african domestic pig cycle: a combined p72-cvr approach. | two of the 22 presently recognised african swine fever (asf) virus p72 genotypes are genetically homogeneous and are associated with domestic pig cycles. of these, genotype viii comprises just two p72 variants, designated 'a' and 'b' in this study, and is confined to four east african countries where it has caused numerous outbreaks between 1961 and 2001. in order to resolve relationships within this homogeneous genotype, the central variable region (cvr) of the 9rl open reading frame of 38 viru ... | 2007 | 17674176 |
| evaluation of a multiplex real-time rt-pcr for quantitative and differential detection of wild-type viruses and c-strain vaccine of classical swine fever virus. | classical swine fever virus (csfv) is the causative agent of classical swine fever (csf), one of oie listed diseases. most of the currently available detection methods do not allow discrimination between wild-type csf viruses and the vaccine strains. this study was designed to develop a multiplex real-time rt-pcr for the quantitative and differential detection of wild-type viruses and c-strain vaccine widely used in china. csfv specific primers and two differently labeled taqman probes for the d ... | 2008 | 17658704 |
| detection of differential expression of porcine ifn-alpha subtypes by reverse transcription polymerase chain reaction. | the porcine interferon-alpha (ifn-alpha) multigene family is a new ifn-alpha system in recent research. characterization of the poifn-alpha multigene family has been described in our previous work, and 14 functional poifn-alpha genes were detected in the porcine genome. in this study, we designed subtype-specific primers and consensus primers for poifn-alpha. the expression of poifn-alpha was detected using the two pcr strategies in three systems, namely, poly(i).poly(c)-deae-dextran-induced pk1 ... | 2007 | 17651019 |
| hog cholera: i. investigation of the agar double-diffusion precipitation test for the detection of the virus in swine tissue. | the agar double-diffusion precipitation test was investigated to detect hog cholera virus in splenic and pancreatic tissues of swine. special attention was given to the conditions influencing the sensitivity and specificity of the test. these studies emphasize the strict techniques and methods required in the test in order to detect specific antigen - antibody reaction. absorption studies performed on serum fractions prepared by deae cellulose chromatography and studied electrophoretically, indi ... | 1964 | 17649544 |
| a soluble precipitating antigen from hog cholera virus propagated in tissue culture: i. preparation and characterization of the antigen. | a precipitating hog cholera viral antigen was produced in a swine kidney tissue culture system. this antigen produced a single, sharp precipitin line with reference antiserum but no precipitin lines with nonimmune control sera. the precipitating antigen was present in the supernatant fluid after high-speed centrifugation, and was readily separated from infective virus by diethylaminoethyl cellulose (deae) chromatography. the antigen was found to be nondialyzable, stable to the action of chlorofo ... | 1964 | 17649522 |
| the properties and classification of bovine viral diarrhea virus. | examination of the c24v (oregon) and mac a (ontario) strains of bovine viral diarrhea viruses have shown them to be ribonucleic acid containing viruses, with essential lipid and having compound helical symmetry with the diameter of the helix being in the neighbourhood of 180 a. because of these properties it is suggested that the virus should be considered a member of the myxovirus group. hog cholera virus is related to bovine viral diarrhea virus by means of a "soluble" antigen, and also posses ... | 1964 | 17649513 |
| use of the agar diffusion precipitation test in the diagnosis of hog cholera. | the application of the agar diffusion precipitation (adp) test for diagnosing hog cholera was investigated. the test used as antigen, pancreatic tissue from 272 pigs that had been inoculated with hog cholera virus. the test was positive for 13.5% of the animals that were sick for 4 days or less, 40% of those sick for 5 days, and 77% of those sick for 6 days or more. the test was positive for 13.5% of all animals that had been vaccinated with crystal violet-glycerol hog cholera vaccine and had be ... | 1963 | 17649474 |
| identification of hog cholera viral antigen by immunofluorescence. application as a diagnostic and assay method. | in the absence of detectable cytologic changes in hog cholera virus-infected tissue culture cells, hog cholera viral antigen was readily detected by immunofluorescence. the ability to detect hog cholera viral antigen by this method allowed for determination of infectivity titers and also for titration of homologous antibody. immunofluorescence made possible the identification, in tissue culture, of hog cholera virus from blood, serum, and spleen extracts of experimentally infected swine. further ... | 1963 | 17649467 |
| precipitation reactions in agar between swine serum and homologous pancreas extracts. | hyperimmune anti-hog cholera and nonimmune swine sera yielded approximately 50% more precipitation reactions in agar-gel diffusion tests with pancreas extracts from spf noninfected swine than with extracts obtained from swine experimentally infected with virulent hog cholera virus. the pancreas-reacting property of swine serum was determined to be relatively heat stable, withstanding 68 c for 30 minutes. of various swine serum fractions tested, the only one that reacted with pancreas extracts co ... | 1963 | 17649466 |
| demonstration of an antigenic relationship between hog cholera and bovine viral diarrhea viruses by immunofluorescence. | specific staining of antigen within bovine embryo kidney tissue culture cells, infected with either oregon c24v or nadl-md bovine viral diarrhea virus, was accomplished using fluorescein-conjugated swine anti-hog cholera or bovine antiviral diarrhea globulin. also specific staining of antigen within pig kidney tissue culture cells, infected with hog cholera virus, was accomplished using the same two types of conjugates. specificity was confirmed by appropriate controls. the authors found immunof ... | 1963 | 17649449 |
| chimeric pestiviruses: candidates for live-attenuated classical swine fever marker vaccines. | the use of attenuated classical swine fever virus (csfv) strains as live vaccines is no longer allowed for the control of classical swine fever in europe, due to the inability to differentiate between infected and vaccinated animals (differentiating infected from vaccinated animals; diva), except as emergency vaccines or as bait vaccines for wild boars. thus, the establishment of a diva vaccine(s) is of pivotal importance for the control of this infectious disease. in this study, recombinant ver ... | 2007 | 17622629 |
| serum concentrations of c-reactive protein, serum amyloid a, and haptoglobin in pigs inoculated with african swine fever or classical swine fever viruses. | to determine serum concentrations of the selected acute-phase proteins (apps) haptoglobin, serum amyloid a (saa), and c-reactive protein (crp) in pigs experimentally inoculated with classical swine fever (csf) and african swine fever (asf) viruses. | 2007 | 17605613 |
| [monitoring of infectious diseases among wild boars]. | sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ... | 2007 | 17601049 |
| efficient priming against classical swine fever with a safe glycoprotein e2 expressing orf virus recombinant (orfv vrv-e2). | an increasing demand in livestock animal husbandry for intervention or emergency vaccination strategies requires a rapid onset of protection linked to prevention of infectious agent spread. using the new recombinant parapoxvirus (ppv) orf virus (orfv) as a vaccine expressing the csfv e2 glycoprotein we demonstrate that a single intra-muscular application confers solid protection. in the prime only concept, multi-site application of the vector vaccine turned out to be superior to single-site appl ... | 2007 | 17600594 |
| a search for structurally similar cellular internal ribosome entry sites. | internal ribosome entry sites (ires) allow ribosomes to be recruited to mrna in a cap-independent manner. some viruses that impair cap-dependent translation initiation utilize ires to ensure that the viral rna will efficiently compete for the translation machinery. ires are also employed for the translation of a subset of cellular messages during conditions that inhibit cap-dependent translation initiation. ires from viruses like hepatitis c and classical swine fever virus share a similar struct ... | 2007 | 17591613 |
| expression and functional characterization of classical swine fever virus e(rns) protein. | e(rns) is an envelope glycoprotein of classical swine fever virus (csfv) with unusual rnase activity. recently, e(rns) was found to have a new function of counteracting the beta-interferon (ifn-beta) induction pathway. in this study, wildtype ernssm and two mutated e(rns) proteins ernsh297k and ernsh346k were expressed in insect cells and purified for rnase activity and function analysis. rnase activity assay in vitro demonstrated that only wildtype e(rns) protein had rnase activity. however, bo ... | 2007 | 17587595 |
| variation in e(rns) viral glycoprotein associated with failure of immunohistochemistry and commercial antigen capture elisa to detect a field strain of bovine viral diarrhea virus. | bovine viral diarrhea virus (bvdv) affects cattle populations causing clinical signs that range from subclinical immunosuppression to severe reproductive and respiratory problems. detection and removal of persistently infected (pi) calves is the single most important factor for control and eradication of bvdv. current testing strategies to detect pi calves rely heavily on immunohistochemistry (ihc) and a commercially available antigen capture elisa (ace) assay. these viral assays depend on 1 or ... | 2007 | 17582710 |
| recombinant e2 glycoprotein of bovine viral diarrhea virus induces a solid humoral neutralizing immune response but fails to confer total protection in cattle. | two recombinant baculoviruses were produced in order to obtain a bovine viral diarrhea virus (bvdv) immunogen: acnpv/e2 expressing e2 glycoprotein, and acnpv/e0e1e2 expressing the polyprotein region coding for the three structural proteins of bvdv (e0, e1, and e2). mice were immunized with sf9 cells infected with the recombinant baculoviruses in a water in oil formulation and the production of neutralizing antibodies was evaluated. since e2 elicited higher neutralizing antibody titers than e0-e1 ... | 2007 | 17581680 |
| african swine fever virus p37 structural protein is localized in nuclear foci containing the viral dna at early post-infection times. | the replication of african swine fever virus dna is initiated inside the nucleus of host cells, being followed by a longer cytoplasmic replication stage. in face of previous results demonstrating the nucleo-cytoplasmic shuttling activity of asfv p37 structural protein when considered isolated from the virus infection, we performed a systematic analysis of the subcellular localization of p37 protein in asfv-infected cells, aiming at identifying the role of the nuclear transport mediated by this p ... | 2007 | 17580096 |
| [alphavirus replicon-vectored plasmid dna-based vaccine elicits protective immunity against classical swine fever virus]. | we have shown previously that a semliki forest virus (sfv) replicon vectored dna vaccine (psfv1cs-e2) expressing the e2 glycoprotein of classical swine fever virus (csfv) conferred full protection for pigs immunized three times with 600 microg of the vaccine. this study aims to evaluate the efficacy of the dna vaccine with lower dosage and fewer inoculations. pigs were immunized twice with 100 microg psfv1cs-e2 (n = 5) or control plasmid psfv1cs (n = 3), respectively. pigs immunized with psfv1cs ... | 2007 | 17577989 |