Publications

design, synthesis, and biological activities of novel azole-bonded β-hydroxypropyl oxime o-ethers.	the synthesis and biological effects of 15 novel azole-bonded β-hydroxypropyl oxime o-ethers have been described. in this synthesis, the oximation of aromatic ketones followed by an o-alkylation reaction with epichlorohydrin and/or epibromohydrin led to the corresponding o-oxime ether adducts. subsequently, the attained o-oxime ether adducts were used to synthesize the target molecules after treating them with the appropriate azole derivatives. the in vitro antifungal and antibacterial activitie ...	2014	25081563
effect of acetic acid on citric acid fermentation in an integrated citric acid-methane fermentation process.	an integrated citric acid-methane fermentation process was proposed to solve the problem of extraction wastewater in citric acid fermentation process. extraction wastewater was treated by anaerobic digestion and then recycled for the next batch of citric acid fermentation to eliminate wastewater discharge and reduce water resource consumption. acetic acid as an intermediate product of methane fermentation was present in anaerobic digestion effluent. in this study, the effect of acetic acid on ci ...	2014	25080378
biological air contamination in elderly care centers: geria project.	indoor air quality (iaq) affects health particularly in susceptible individuals such as the elderly. it has been estimated that the older population spends approximately 19-20 h/d indoors, and the majority of the elderly spend all of their time indoors in elderly care centers (ecc). older individuals may be particularly at risk of exposure to detrimental effects from pollutants, even at low concentrations, due to common and multiple underlying chronic diseases that increase susceptibility. this ...	2014	25072726
nitrate reductase (15)n discrimination in arabidopsis thaliana, zea mays, aspergillus niger, pichea angusta, and escherichia coli.	stable (15)n isotopes have been used to examine movement of nitrogen (n) through various pools of the global n cycle. a central reaction in the cycle involves the reduction of nitrate (no(-) 3) to nitrite (no(-) 2) catalyzed by nitrate reductase (nr). discrimination against (15)n by nr is a major determinant of isotopic differences among n pools. here, we measured in vitro (15)n discrimination by several nrs purified from plants, fungi, and a bacterium to determine the intrinsic (15)n discrimina ...	2014	25071800
influence of the pesticides glyphosate, chlorpyrifos and atrazine on growth parameters of nonochratoxigenic aspergillus section nigri strains isolated from agricultural soils.	this investigation was undertake to determine the effect of glyphosate, chlorpyrifos and atrazine on the lag phase and growth rate of nonochratoxigenic a. niger aggregate strains growing on soil extract medium at -0.70, -2.78 and -7.06 mpa. under certain conditions, the glyphosate concentrations used significantly increased micelial growth as compared to control. an increase of about 30% was observed for strain an 251 using 5 and 20 mg l(-1) of glyphosate at -2.78 mpa. the strains behaved differ ...	2014	25065826
enzymatic hydrolysis of esters containing a tetrazole ring.	the lipase-catalyzed enantioselective hydrolysis of acetates containing tetrazole moiety was studied. among all tested lipases, novozyme sp 435 allowed to obtain optically active 4-(5-aryl-2h-tetrazol-2yl)butan-2-ol and 1-(5-aryl-2h-tetrazol-2yl)-propan-2-ol and their acetates with the highest optical purities (ee = 95%-99%) and excellent enantioselectivity (e>100). some of the synthesized tetrazole derivatives were screened for their antifungal activity. racemic mixtures of 4-[5-(4-chlorophenyl ...	2014	25065812
nucleotide and amino acid variations of tannase gene from different aspergillus strains.	tannase is an enzyme that catalyses the hydrolysis of ester bonds present in tannins. most of the scientific reports about this biocatalysis focus on aspects related to tannase production and its recovery; on the other hand, reports assessing the molecular aspects of the tannase gene or protein are scarce. in the present study, a tannase gene fragment from several aspergillus strains isolated from the mexican semidesert was sequenced and compared with tannase amino acid sequences reported in ncb ...	2014	25065666
similar is not the same: differences in the function of the (hemi-)cellulolytic regulator xlnr (xlr1/xyr1) in filamentous fungi.	the transcriptional activator xlnr (xlr1/xyr1) is a major regulator in fungal xylan and cellulose degradation as well as in the utilization of d-xylose via the pentose catabolic pathway. xlnr homologs are commonly found in filamentous ascomycetes and often assumed to have the same function in different fungi. however, a comparison of the saprobe aspergillus niger and the plant pathogen magnaporthe oryzae showed different phenotypes for deletion strains of xlnr. in this study wild type and xlnr/x ...	2014	25064064
fungal biodegradation of dibutyl phthalate and toxicity of its breakdown products on the basis of fungal and bacterial growth.	phthalates are esters of phthalic acid that give flexibility to polyvinyl chloride. diverse studies have reported that these compounds might be carcinogenic, mutagenic and/or teratogenic. radial growth rate, biomass, hyphal thickness of neurospora sitophyla, trichoderma harzianum and aspergillus niger, grown in two different concentrations of dibutyl phthalate (dbp) (500 and 1,000 mg/l) in agar and in submerged fermentation were studied. the inhibitory concentration (ic50) and the constant of bi ...	2014	25063688
germination of aspergillus niger conidia is triggered by nitrogen compounds related to l-amino acids.	conidial germination is fundamentally important to the growth and dissemination of most fungi. it has been previously shown (k. hayer, m. stratford, and d. b. archer, appl. environ. microbiol. 79:6924-6931, 2013, http://dx.doi.org/10.1128/aem.02061-13), using sugar analogs, that germination is a 2-stage process involving triggering of germination and then nutrient uptake for hyphal outgrowth. in the present study, we tested this 2-stage germination process using a series of nitrogen-containing c ...	2014	25063657
genome mining and functional genomics for siderophore production in aspergillus niger.	iron is an essential metal for many organisms, but the biologically relevant form of iron is scarce because of rapid oxidation resulting in low solubility. simultaneously, excessive accumulation of iron is toxic. consequently, iron uptake is a highly controlled process. in most fungal species, siderophores play a central role in iron handling. siderophores are small iron-specific chelators that can be secreted to scavenge environmental iron or bind intracellular iron with high affinity. a second ...	2014	25062661
effects of high pressure homogenization on the activity, stability, kinetics and three-dimensional conformation of a glucose oxidase produced by aspergillus niger.	high pressure homogenization (hph) is a non-thermal method, which has been employed to change the activity and stability of biotechnologically relevant enzymes. this work investigated how hph affects the structural and functional characteristics of a glucose oxidase (go) from aspergillus niger. the enzyme was homogenized at 75 and 150 mpa and the effects were evaluated with respect to the enzyme activity, stability, kinetic parameters and molecular structure. the enzyme showed a ph-dependent res ...	2014	25061935
physical, mechanical and antimicrobial properties of starch films incorporated with ε-poly-l-lysine.	starch/ε-poly-l-lysine (ε-pl) composite films were prepared by combining 4% (w/v) gelatinized cornstarch and varying the level of ε-pl. the physical, mechanical and antimicrobial properties of these films were investigated. fourier-transform infrared spectra (ft-ir) showed that the carbonyl group stretching vibration band of the ε-pl molecule shifted from 1646 cm(-1) to 1673 cm(-1) in the composite films. differential scanning calorimetry (dsc) results indicated that there were sharp endothermal ...	2015	25053035
compositional studies and biological activities of perovskia abrotanoides kar. oils.	current study has been designed to evaluate the chemical composition of essential and fixed oils from stem and leaves of perovskia abrotanoides and antioxidant and antimicrobial activities of these oils.	2014	25052892
[investigation of acid proteinase and phospholipase activity as virulence factors in clinical aspergillus spp. isolates].	aspergillus spp. are widespread in nature and cause severe infections especially in immunocompromised patients. aspergillus fumigatus complex is the most common species that causes infections in humans; however aspergillus niger complex and aspergillus flavus complex are the emerging agents that are isolated frequently from clinical specimens more recently. besides the host factors such as immunosuppression, hematologic malignancy and corticosteroid use, fungal virulence factors such as elastase ...	2014	25052116
antifungal activity and chemical composition of twenty essential oils against significant indoor and outdoor toxigenic and aeroallergenic fungi.	health affecting, loss-inducing or otherwise harmful fungal pathogens (molds) pose a serious challenge in many areas of human activities. on the contrary, frequent use of synthetic fungicides is undesirable in some cases and may be equally problematic. moreover, the ever more increasing fungal resistance against commercial synthetic fungicides justifies development of rising efforts to seek new effective, while environmentally friendly alternatives. botanical fungicides based on essential oils ( ...	2014	25048938
comparative study of toxicity of azo dye procion red mx-5b following biosorption and biodegradation treatments with the fungi aspergillus niger and aspergillus terreus.	azo dyes are an important class of environmental contaminants and are characterized by the presence of one or more azo bonds (-n=n-) in their molecular structure. effluents containing these compounds resist many types of treatments due to their molecular complexity. therefore, alternative treatments, such as biosorption and biodegradation, have been widely studied to solve the problems caused by these substances, such as their harmful effects on the environment and organisms. the aim of the pres ...	2014	25048922
type iii polyketide synthase is involved in the biosynthesis of protocatechuic acid in aspergillus niger.	genomic studies have shown that not only plants but also filamentous fungi contain type iii polyketide synthases. to study the function of type iii polyketide synthase (anpksiii) in aspergillus niger, a deletion strain (delanpksiii) and an overexpression strain (oeanpksiii) were constructed in a. niger ma169.4, a derivative of the wild-type (wt) a. niger atcc 9029 that produces large quantities of gluconic acid. alterations in the metabolites were analyzed by hplc when the extract of the overexp ...	2014	25048233
three acyltetronic acid derivatives: noncanonical cryptic polyketides from aspergillus niger identified by genome mining.	induced production of tetronate derivatives: we activated a dormant pks-nrps gene cluster in aspergillus niger atcc 1015 by expressing its dedicated transcription activator (caar). as a result, the transformant expressing caar produces three acyltetronate derivatives: carlosic acid (1), agglomerin f (2), and carlosic acid methyl ester (3).	2014	25044953
enhanced bioremediation of soil from tianjin, china, contaminated with polybrominated diethyl ethers.	this work aimed to evaluate the effectiveness of nutrients, h2o2, and tourmaline on the bioremediation of fields where the soil was contaminated with polybrominated diethyl ethers (pbdes). the results showed that 39.2, 38.3, and 48.1 % of total pbde removal was observed in microcosms with the addition of nutrients, such as nano3, nh4cl, and nh4no3, respectively, compared to only 15.2 and 5.8 % of pbde removal from soil with added aspergillus niger and control soil, respectively, after 50 days of ...	2014	25043595
co-cultivation of trichoderma reesei rutc30 with three black aspergillus strains facilitates efficient hydrolysis of pretreated wheat straw and shows promises for on-site enzyme production.	co-cultivation of fungi may be an excellent system for on-site production of cellulolytic enzymes in a single bioreactor. enzyme supernatants from mixed cultures of trichoderma reesei rutc30, with either the novel aspergillus saccharolyticus ap, aspergillus carbonarius item 5010 or aspergillus niger cbs 554.65 cultivated in solid-state fermentation were tested for avicelase, fpase, endoglucanase and beta-glucosidase activity as well as in hydrolysis of pretreated wheat straw. around 30% more avi ...	2014	25043347
a combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts.	a reliable method to determine cell wall polysaccharides composition in yeast is presented, which combines acid and enzymatic hydrolysis. sulphuric acid treatment is used to determine mannans, whereas specific hydrolytic enzymes are employed in a two sequential steps to quantify chitin and the proportion of β-(1,3) and β-(1,6)-glucan in the total β-glucan of the cell wall. in the first step, chitin and β-(1,3)-glucan were hydrolysed into their corresponding monomers n-acetylglucosamine and gluco ...	2014	25041403
bacillus subtilis attachment to aspergillus niger hyphae results in mutually altered metabolism.	interaction between microbes affects the growth, metabolism and differentiation of members of the microbial community. while direct and indirect competition, like antagonism and nutrient consumption have a negative effect on the interacting members of the population, microbes have also evolved in nature not only to fight, but in some cases to adapt to or support each other, while increasing the fitness of the community. the presence of bacteria and fungi in soil results in various interactions i ...	2015	25040940
gene encoding a novel invertase from a xerophilic aspergillus niger strain and production of the enzyme in pichia pastoris.	β-fructofuranosidases or invertases (ec 3.2.1.26) are enzymes that are widely used in the food industry, where fructose is preferred over sucrose, because it is sweeter and does not crystallize easily. since aspergillus niger gh1, an xerophilic fungus from the mexican semi-desert, has been reported to be an invertase producer, and because of the need for new enzymes with biotechnological applications, in this work, we describe the gene and amino acid sequence of the invertase from a. niger gh1, ...	2014	25039056
synthesis of mannich bases: 2-(3-phenylaminopropionyloxy)-benzoic acid and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one, their toxicity, ionization constant, antimicrobial and antioxidant activities.	mannich bases 2-(3-phenylaminopropionyloxy)-benzoic acid (a) and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one (b) were synthesized. structures were confirmed by ultraviolet/visible and infra-red spectroscopies. the ionization constant (pka) values at 8.3 and 8.0 reported for compounds a and b, respectively, indicated that protonation might occur at physiological ph. the lc50 values of 145,595 μg/ml (a) and 82,526 μg/ml (b) obtained from brine shrimp lethality testing showed that both c ...	2014	25038706
expression of a codon-optimized aspergillus niger pectin methylesterase gene in the methylotrophic yeast candida boidinii.	a codon-optimized aspergillus niger pectin methylesterase (pme) gene was expressed in the methylotrophic yeast canidia boidinii. the pme-producing strains showed better growth on pectin than the wild-type strains, suggesting that the pme-producing strains could efficiently utilize methyl ester moieties of pectin. on the other hand, overproduction of pme negatively affected the proliferation of c. boidinii on leaves of arabidopsis thaliana.	2014	25036973
the use of real-time pcr to study penicillium chrysogenum growth kinetics on solid food at different water activities.	fungal growth on solid foods can make them unfit for human consumption, but certain specialty foods require fungi to produce their characteristic properties. in either case, a reliable way of measuring biomass is needed to study how various factors (e.g. water activity) affect fungal growth rates on these substrates. biochemical markers such as chitin, glucosamine or ergosterol have been used to estimate fungal growth, but they cannot distinguish between individual species in mixed culture. in t ...	2014	25036772
microbial transformations of 3-methoxyflavone by strains of aspergillus niger.	microbial transformation of 3-methoxyflavone into 3'-hydroxyflavon-3-yloxymethyl myristate was presented. six filamentous fungi were used as biocatalysts: a wild strain of aspergillus niger kb, its four uv mutants (a. niger mb, sbp, sbj, 13/5) and the strain of penicillium chermesinum 113. the highest yields were observed for the strains of a. niger kb and a. niger sbp (69.8% and 63.1%, respectively).	2014	25033671
growth kinetics and mechanistic action of reactive oxygen species released by silver nanoparticles from aspergillus niger on escherichia coli.	silver nanoparticles (agnps), the real silver bullet, are known to have good antibacterial properties against pathogenic microorganisms. in the present study agnps were prepared from extracellular filtrate of aspergillus niger. characterization of agnps by uv-vis spectrum reveals specific surface plasmon resonance at peak 416 nm; tem photographs revealed the size of the agnps to be 20-55 nm. average diameter of the produced agnps was found to be 73 nm with a zeta potential that was -24 mv using ...	2014	25028666
composition, antioxidant and antimicrobial activities of methanol extracts of some acinos miller species.	gc and gc/ms analyses of the methanol extracts obtained from the aerial parts of six acinos miller species from serbia and montenegro were performed. seventy-four constituents, accounting for 84.9-99.0% of the total composition of the extracts, were identified. the common feature of the a. suaveolens, a. majoranifolius and a. alpinus methanol extracts was the high content of monoterpenes, while the common feature of the a. graveolens, a. arvensis and a. hungaricus methanol extracts was the preva ...	2014	25026735
antifungal agents against aspergillus niger for rearing rice leaffolder larvae (lepidoptera: pyralidae) on artificial diet.	mold contamination is an important issue in insect mass rearing. frequently used antifungal agents such as sorbic acid and methylparaben have negative impact on many lepidopteran larvae, which might be one of the reasons for the difficulty in rearing rice leaffolder, cnaphalocrocis medinalis (güenée). in this study, 19 antifungal agents, including 7 food preservatives, 6 antifungal drugs, and 6 agricultural fungicides, were screened for their inhibitory activities on aspergillus niger in diets. ...	2014	25026669
invasive primary aspergillosis of the larynx presenting as hoarseness and a chronic nonhealing laryngeal ulcer in an immunocompetent host: a rare entity.	primary aspergillosis usually affects the paranasal sinuses, orbit, ear, and lower respiratory tract. laryngeal aspergillosis usually occurs as a result of secondary invasion from the tracheobronchial tree, more commonly in immunocompromised hosts. primary laryngeal localization of aspergillus infection is seldom encountered. we report the case of an immunocompetent 42-year-old man who presented with hoarseness and a laryngeal ulcer of fairly long duration. a malignancy was initially suspected c ...	2014	25025412
characterisation and functional properties of antimicrobial bio-barriers formed by natural fibres.	antimicrobial bio-barriers formed on cotton (co), silk (se), and woollen (wo) fabrics were prepared by the application of 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride (si-qac) at 11 concentrations ranging from 0.5% to 20% using an exhaustion method. the presence of the si-qac coating on the treated fabric samples was detected by x-ray photoelectron spectroscopy. the bromophenol blue reagent was used to determine the concentration of quaternary ammonium groups in the coating. the ...	2014	25024108
[chronic necrotizing pulmonary aspergillus niger aspergillosis in a smoker and former tb patient].		2014	25018830
modeling growth of three bakery product spoilage molds as a function of water activity, temperature and ph.	the objective of this study was to quantify the effect of water activity, ph and storage temperature on the growth of eurotium repens, aspergillus niger and penicillium corylophilum, isolated from spoiled bakery products. moreover, the behaviors of these three mold species were compared to assess whether a general modeling framework may be set and re-used in future research on bakery spoilage molds. the mold growth was modeled by building two distinct gamma-type secondary models: one on the lag ...	2014	25016208
by-products from the biodiesel chain as a substrate to citric acid production by solid-state fermentation.	in this study, we propose the use of tung cake for the production of organic acids, with an emphasis on citric acid by solid-state fermentation. we evaluated the conditions of production and the by-products from the biodiesel chain as raw materials involved in this bioprocess. first, we standardized the conditions of solid-state fermentation in tung cake with and without residual fat and with different concentrations of glycerine using the fungus aspergillus niger the solid-state fermentation pr ...	2014	25012861
for the inactivation of mold spores by uvc irradiation, with ozone acting as a promoter, tio2 nanoparticles may act better as a "sun block" than as a photocatalytic disinfectant.	fungal spores are known as critical indoor allergens, and indoor air purification techniques including photocatalytic disinfection using titanium dioxide (tio2), ultraviolet germicidal irradiation (uvgi) and ozonation, have been considerably investigated. however, most of the research is in regard to photocatalytic disinfection, focused on the anti-bacterial efficacy of tio2 nanoparticles (nps). furthermore, some research even showed that the photocatalytic antifungal efficacy of tio2 nps may no ...	2014	25007943
biological detoxification of zearalenone by aspergillus niger strain fs10.	zearalenone (zen) contamination of corn and cereal products is a serious health hazard throughout the world and its elimination by microbial methods is now being widely examined. in this study, an aspergillus niger strain, fs10, isolated from chinese fermented soybean, was shown to reduce levels of zen in corn steep liquor (csl). spores, mycelium and culture filtrate of the strain fs10 were tested for their ability to remove zen. the results indicated that strain fs10 could remove 89.56% of zen ...	2014	25007785
determination of the ph dependent redox potential of glucose oxidase by spectroelectrochemistry.	the ph dependent redox potential of the oxidoreductase glucose oxidase (gox) from aspergillus niger, which is the most frequently applied enzyme in electrochemical glucose biosensors and biofuel cells, was measured between ph 4.5 and 8.5 using uv/vis spectroelectrochemistry. in the entire ph range under investigation, the flavin adenine dinucleotide cofactor of gox changed directly from the oxidized quinone to the doubly reduced hydroquinone. no stable semiquinoid species could be detected if el ...	2014	25007396
agent-based modeling of malaria vectors: the importance of spatial simulation.	the modeling of malaria vector mosquito populations yields great insight into drivers of malaria transmission at the village scale. simulation of individual mosquitoes as "agents" in a distributed, dynamic model domain may be greatly beneficial for simulation of spatial relationships of vectors and hosts.	2014	24992942
synthesis and biological evaluation of novel n-aryl maleimide derivatives clubbed with α-hydroxyphosphonates.	a series of novel molecules 5a-g containing n-aryl maleimide and α-hydroxyphosphonate moieties were synthesized. a distinct approach for high-yielding synthesis of α-hydroxyphosphonates has been discovered using various catalyst and solvents. the structures of the synthesized compounds were elucidated by ir, nmr, ms and chn analysis. all the synthesized compounds were tested for qualitative (zone of inhibition) and quantitative (mic) antimicrobial activities against two pathogenic bacteria such ...	2014	24992076
assessment of allergenicity to fungal allergens of rohtak city, haryana, india.	fungal spores are known as one of the important bioparticles causing allergic manifestation in human beings. hence, knowledge of season and prevalence of the airborne allergens to which the patients are exposed is a prerequisite for proper diagnosis and treatment of allergic disorders in hypersensitive individuals. keeping this in view, aerial survey was performed in the atmosphere of rohtak city for 2 consecutive years (march 2008-february 2010), using a volumetric petri plate sampler. a total ...	2014	24988378
mixed disulfide formation at cys141 leads to apparent unidirectional attenuation of aspergillus niger nadp-glutamate dehydrogenase activity.	nadp-glutamate dehydrogenase from aspergillus niger (angdh) exhibits sigmoid 2-oxoglutarate saturation. incubation with 2-hydroxyethyl disulfide (2-hed, the disulfide of 2-mercaptoethanol) resulted in preferential attenuation of angdh reductive amination (forward) activity but with a negligible effect on oxidative deamination (reverse) activity, when monitored in the described standard assay. such a disulfide modified angdh displaying less than 1.0% forward reaction rate could be isolated after ...	2014	24987966
targeted lipid analysis of haemolytic mycelial extracts of aspergillus niger.	ethanolic extracts of mycelia from aspergillus niger (strain n402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. this haemolytic activity decreased significantly during the time of growth (1-3 days). moreover, when a. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-depriv ...	2014	24983857
acute lymphocytic leukemia with superimposed invasive aspergillosis and pneumopericardium successfully treated with voriconazole.	we present a 47-year-old man with acute lymphocytic leukemia with a pericardial friction rub heralding pericardial aspergillosis. the clinical course was complicated by pneumopericardium, likely secondary to a direct connection between the lung parenchyma and the pericardial space. bronchoalveolar lavage cultures returned positive for methicillin-resistant staphylococcus aureus and aspergillus niger. combination voriconazole and vancomycin resulted in symptomatic improvement within 2 weeks of ho ...	2014	24982579
experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases.	polygalacturonases (pgs) are hydrolytic enzymes employed by several phytopathogens to weaken the plant cell wall by degrading homopolygalacturonan, a major constituent of pectin. plants fight back by employing polygalacturonase-inhibitor proteins (pgips). the present study compared the inhibition potential of pearl millet pgip (pennisetum glaucum; pglpgip1) with the known inhibition of phaseolus vulgaris pgip (pvpgip2) against two pgs, the pg-ii isoform from aspergillus niger (anpgii) and the pg ...	2014	24980909
purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (solanum tuberosum).	a new chitin-binding lectin was purified from a bangladeshi cultivar 'deshi' of potato (solanum tuberosum l.) through anion-exchange and affinity chromatographies using a chitin column. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed the molecular mass of the lectin as 20,000 daltons. this molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. the lectin showed both bactericidal and growth-inhibiting activities a ...	2014	24980018
influence of albizia lebbeck saponin and its fractions on in vitro gas production kinetics, rumen methanogenesis, and rumen fermentation characteristics.	the present study was undertaken to investigate the effect of crude seed powder (csp) and gross saponins extract (gse) of seeds of albizia lebbeck on antimicrobial activity by taking two gram-positive (staphylococcus aureus and bacillus cereus), two gram-negative (escherichia coli and salmonella typhi) bacteria, and two fungi species (aspergillus niger and candida butyric) were taken at 25, 50, 100, 250, and 500 µg levels using agar well diffusion method. zone of inhibition was increased with in ...	2014	24977047
production and characterization of in planta transiently produced polygalacturanase from aspergillus niger and its fusions with hydrophobin or elp tags.	pectinases play an important role in plant cell wall deconstruction and have potential in diverse industries such as food, wine, animal feed, textile, paper, fuel, and others. the demand for such enzymes is increasing exponentially, as are the efforts to improve their production and to implement their use in several industrial processes. the goal of this study was to examine the potential of producing polygalacturonase i from aspergillus niger in plants and to investigate the effects of subcellu ...	2014	24970673
a new member of the dmats superfamily from aspergillus niger catalyzes prenylations of both tyrosine and tryptophan derivatives.	a putative prenyltransferase gene of the dimethylallyltryptophan synthase (dmats) family, an13g01840, was identified in the genome sequence of aspergillus niger. the deduced polypeptide cak41583 consists of 465 amino acids with a calculated molecular mass of 52.7 kda. to evaluate gene function, the coding sequence was cloned into pet28a and overexpressed in escherichia coli. the soluble his6-fusion protein was purified to near homogeneity on ni-nta agarose and used for enzyme assays with diverse ...	2014	24970457
fungal infections among diabetic foot ulcer- patients attending diabetic clinic in kenyatta national hospital, kenya.	to isolate and identify fungal pathogens associated with dermatophytoses in diabetic patients and identify the spectrum of yeasts colonising diabetic foot ulcers at kenyatta national hospital.	2011	24968597
a novel glucose dehydrogenase from the white-rot fungus pycnoporus cinnabarinus: production in aspergillus niger and physicochemical characterization of the recombinant enzyme.	data on glucose dehydrogenases (gdhs) are scarce and availability of these enzymes for application purposes is limited. this paper describes a new gdh from the fungus pycnoporus cinnabarinus cirm brfm 137 that is the first reported gdh from a white-rot fungus belonging to the basidiomycota. the enzyme was recombinantly produced in aspergillus niger, a well-known fungal host producing an array of homologous or heterologous enzymes for industrial applications. the full-length gene that encodes gdh ...	2014	24965558
genome-scale analysis of the high-efficient protein secretion system of aspergillus oryzae.	the koji mold, aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. however, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome.	2014	24961398
hemicellulase production by aspergillus niger dsm 26641 in hydrothermal palm oil empty fruit bunch hydrolysate and transcriptome analysis.	palm oil empty fruit bunches (efb) is an abundant and cheap lignocellulose material in southeast asia. its use as the sole medium for producing lignocellulose-hydrolyzing enzymes would increase its commercial value. a newly isolated aspergillus niger dsm 26641 was investigated for its capability of producing hemicellulases in efb hydrolysate obtained by treatment with pressurized hot water (1-20%, w/v) at 120-180°c in a 1 l parr reactor for 10-60 min. the optimal hydrolysate for the fungal growt ...	2014	24958131
one pot three components microwave assisted and conventional synthesis of new 3-(4-chloro-2-hydroxyphenyl)-2-(substituted) thiazolidin-4-one as antimicrobial agents.	a one-pot, three-component, microwave assisted and conventional synthesis of new 3-(4-chloro-2-hydroxyphenyl)-2-(substituted) thiazolidin-4-one (4a-n) was carried out by using n,n-dimethylformamide as a solvent with high product yield. among these synthesized compounds (4f, 4g, 4l and 4m) were found to be a broad spectrum molecule active against all bacterial and fungus strains tested, except fungus aspergillus niger. amongst the compounds (4g, 4l and 4m) were found to be more potent than respec ...	2014	24951333
conversion of orange peel to l-galactonic acid in a consolidated process using engineered strains of aspergillus niger.	citrus processing waste is a leftover from the citrus processing industry and is available in large amounts. typically, this waste is dried to produce animal feed, but sometimes it is just dumped. its main component is the peel, which consists mostly of pectin, with d-galacturonic acid as the main monomer. aspergillus niger is a filamentous fungus that efficiently produces pectinases for the hydrolysis of pectin and uses the resulting d-galacturonic acid and most of the other components of citru ...	2014	24949267
primary otomycosis in the indian subcontinent: predisposing factors, microbiology, and classification.	objective. to define otomycosis and determine the predisposing factors and microbiology in primary otomycosis. study design. prospective study of two years and review of the literature. setting. academic department of otolaryngology in a coastal city in india. patients. 150 immunocompetent individuals of whom 100 consecutive patients with a clinical diagnosis of otomycosis are considered as the study group and 50 consecutive patients with no otomycosis are considered as the control group. result ...	2014	24949016
structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme.	ochratoxin, with ochratoxin a as the dominant form, is one of the five major mycotoxins most harmful to humans and animals. it is produced by aspergillus and penicillium species and occurs in a wide range of agricultural products. detoxification of contaminated food is a challenging health issue. in the present paper we report the identification, characterization and crystal structure (at 2.2 å) of a novel microbial ochratoxinase from aspergillus niger. a putative amidase gene encoding a 480 ami ...	2014	24947135
characterization of β -glucosidase produced by aspergillus niger under solid-state fermentation and partially purified using manae-agarose.	β -glucosidase (bgl) is a hydrolytic enzyme with specificity for a wide variety of glycoside substrates, being an enzyme with a large range of biotechnological applications. however, enzyme properties can be different depending both on the microorganism and the cultivation procedure employed. therefore, in order to explore potential biocatalytical applications of novel enzymes, their characterization is essential. in this work, a bgl synthesized by a selected strain of aspergillus niger cultivat ...	2014	24940510
colourimetric and fluorometric substrates for measurement of pullulanase activity.	specific and highly sensitive colourimetric and fluorometric substrate mixtures have been prepared for the measurement of pullulanase and limit-dextrinase activity and assays employing these substrates have been developed. these mixtures comprise thermostable α- and β-glucosidases and either 4,6-o-benzylidene-2-chloro-4-nitrophenyl-β-maltotriosyl (1-6) α-maltotrioside (bzcnpg3g3, 1) as a colourimetric substrate or 4,6-o-benzylidene-4-methylumbelliferyl-β-maltotriosyl (1-6) α-maltotrioside (bzmug ...	2014	24938640
effect of binders on airborne microorganism inactivation using tio2 photocatalytic fluorescent lamps.	5% degussa p25 tio2 was spray-coated onto black-light and white-light fluorescent lamps, using five different binders, namely duramax b-1000, duramax d-3005, silane-69, and two polyethylene glycols with molecular weight 1000 (peg-1000) and 700 (peg-700). the coated lamps were tested with staphylococcus epidermidis, escherichia coli, spores of bacillus subtilis and spores of aspergillus niger. it was found that 0.5% b-1000 and 1% peg-1000 gave the highest inactivation rates: 93-96% from coated bl ...	2014	24937435
enzymatic treatment of specimens before dna extraction directly influences molecular detection of infectious agents.	biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by dna amplification techniques (pcr). moreover the targeted dna has to be released from the complex cell walls and the compact nucleoprotein matrixes and cleared from potential inhibitors. the goal of the present work was to assess the efficiency of enzymatic pretreatments on infectious agents to make dna available for further extraction and ...	2014	24936792
nanomechanical sensors for single microbial cell growth monitoring.	a nanomechanical technique for rapid real time detection and monitoring of microorganism growth will significantly reduce costs and diagnosis times in industrial and clinical settings. owing to their label free detection mechanism and unprecedented sensitivity to the mass and elastic modulus of biological structures, dynamically operated cantilever arrays provide an opportunity to rapidly detect and track the evolution of microbial growth. here we report the monitoring of the growth of single as ...	2014	24931547
in vitro evaluation of combination of polyenes with edta against aspergillus spp. by different methods (fici and ci model).	we investigated in vitro activity of polyene drugs (amphotericin b, amp b; and nystatin, nys;) in combination with ethylene diamine tetra acetic acid (edta) against 8 pathogenic isolates of aspergillus spp.	2014	24931000
mixed reverse micelles facilitated downstream processing of lipase involving water-oil-water liquid emulsion membrane.	our earlier work for the first time demonstrated that liquid emulsion membrane (lem) containing reverse micelles could be successfully used for the downstream processing of lipase from aspergillus niger. in the present work, we have attempted to increase the extraction and purification fold of lipase by using mixed reverse micelles (mrm) consisting of cationic and nonionic surfactants in lem. it was basically prepared by addition of the internal aqueous phase solution to the organic phase follow ...	2015	24930827
microwave assisted synthesis, characterization and biocidal activities of some new chelates of carbazole derived schiff bases of cadmium and tin metals.	this study is planned to report the advancement of green microwave approach in the fabrication of a new series of biologically potent (n^x, where x=o/s) donor schiff bases and their cadmium(ii) and tin(ii) complexes. the ligands and their metal complexes have been characterized in terms of elemental analysis, molar ionic conductance, magnetic moment and spectral (ir, uv-vis, nmr ((1)h, (119)sn), fab-mass, thermal and xrd) data. the data revealed that the ligands coordinated to the metal center v ...	2014	24929756
lag time for germination of penicillium chrysogenum conidia is induced by temperature shifts.	in the environment, fungal conidia are subject to transient conditions. in particular, temperature is varying according to day/night periods. all predictive models for germination assume that fungal spores can adapt instantaneously to changes of temperature. the only study that supports this assumption (gougouli and koutsoumanis, 2012, modelling germination of fungal spores at constant and fluctuating temperature conditions. international journal of food microbiology, 152: 153-161) was carried o ...	2014	24929731
antifungal effect of antimicrobial peptides (amps lr14) derived from lactobacillus plantarum strain lr/14 and their applications in prevention of grain spoilage.	the concern for food safety has led to an increased interest in the development of novel antimicrobials. keeping this aim in mind, we have investigated the antifungal effect of antimicrobial peptides (amps lr14) produced by lactobacillus plantarum strain lr/14 against four spoilage fungi, namely, aspergillus niger, rhizopus stolonifer, mucor racemosus and penicillium chrysogenum. interestingly, all the four fungi were inhibited, suggesting that amps lr14 exhibited anti-fungal property. the pepti ...	2014	24929709
influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities.	the results given in the literature are conflicting when considering the relationship between antimicrobial activity and chitosan characteristics. to be able to clarify, we prepared fifteen homogeneous chitosans with different acetylation degrees (da) and molecular weights (mw) by reacetylation of a fully deacetylated chitin under homogeneous conditions. they were tested at different ph values for their antimicrobial activities against four gram-negative bacteria (escherichia coli, pseudomonas a ...	2014	24929684
evaluation of noninvasive methods for the diagnosis of fungal endocarditis.	fungal endocarditis (fe) is an uncommon disease with a high risk of morbidity and mortality. here, we evaluated the different methods for diagnosing this infection. cardiac valve, vegetation, and embolic materials obtained during surgery were examined for fungal infections by direct smear and culture. at least two blood samples were inoculated at the bedside into bactec medium. galactomannan, mannan ag enzyme-linked immunosorbent assay, and real-time polymerase chain reaction (pcr) assay were pe ...	2014	24915853
diverse and bioactive endophytic aspergilli inhabit cupressaceae plant family.	aspergilli are filamentous, cosmopolitan and ubiquitous fungi which have significant impact on human, animal and plant welfare worldwide. due to their extraordinary metabolic diversity, aspergillus species are used in biotechnology for the production of a vast array of biomolecules. however, little is known about aspergillus species that are able to adapt an endophytic lifestyle in cupressaceae plant family and are capable of producing cytotoxic, antifungal and antibacterial metabolites. in this ...	2014	24912659
improving 2-phenylethanol and 6-pentyl-α-pyrone production with fungi by microparticle-enhanced cultivation (mpec).	trichoderma atroviride imi 206040 synthesizes the coconut lactone 6-pentyl-α-pyrone (6-pap) de novo and aspergillus niger dsm 821 produces the rose-like flavour compound 2-phenylethanol (2-pe) from the precursor l-phenylalanine. here, microparticles of different chemical composition and nominal particle diameter in the range 5-250 µm were added to shake-flask cultures of both fungi to investigate the particles' effect on product formation. maximum 2-pe concentration increased by a factor of 1.3 ...	2015	24910400
fungal mediated generation of mammalian metabolites of fenofibrate and enhanced pharmacological activity of the main metabolite fenofibric acid.	different fungi viz. aspergillus niger ncim 589, a.ochraceous ncim 1140, cunninghamella blakesleeana ncim 687, c. echinulata ncim 691, rhizopus stolonifer ncim 880, mucor rouxi mtcc 386, trichothecium roseum ncim 1147 were screened for their potential to biotransform anti-hyperlipidemia and anti-hypertriglyceridemia drug, fenofibrate to fenofibric acid, the active metabolite and other mammalian metabolites. among the fungi screened c. blakesleeana transformed fenofibrate to fenofibric acid and o ...	2014	24910236
recycling of cell culture and efficient release of intracellular fructosyltransferase by ultrasonication for the production of fructooligosaccharides.	production of fructooligosaccharide (fos) through efficient cultivation of biotransformation process by fructosyltransferase (ftase) was evaluated by two new isolates, aspergillus niger and aspergillus flavus nfcci 2364. the saccharide consumption revealed lag phase of a. niger in 10 h which were smaller extent than a. flavus of 14 h. for the recycling of cell culture, the pellet cells were continuously reused after 24 h of submerged fermentation by these microorganisms in which ftase activity r ...	2014	24906753
effect of potassium sorbate on antimicrobial and physical properties of starch-clay nanocomposite films.	using fresh foods which undergo the least processing operations developed widely in recent years. active packaging is a novel method for preserving these products. active starch-clay nanocomposite films which contained potassium sorbate (ps) at a level of 0, 5, 7.5 and 10 g ps/100 g starch were produced and their physical, mechanical and antimicrobial properties were evaluated. in order to evaluate antimicrobial properties of films aspergillus niger was used. the results showed that 5% of the ps ...	2014	24906724
treatment of both native and deamidated gluten peptides with an endo-peptidase from aspergillus niger prevents stimulation of gut-derived gluten-reactive t cells from either children or adults with celiac disease.	celiac disease (cd) is characterized by an inappropriate immunological reaction against gluten driven by gluten-specific cd4+ t cells. we screened 25 proteases and tested 10 for their potential to degrade gluten in vitro. five proteases were further tested for their ability to prevent the proliferative response by a gluten-specific cd4+ t cell clone and seven gluten-reactive t cell lines to protease-digested gluten peptides. a proline-specific endo-peptidase from aspergillus niger (anp2) was par ...	2014	24905137
screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation.	seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ...	2014	24894549
antifungal effect of gatifloxacin and copper ions combination.	it is a well-known fact that to bring a new molecule it may take more than a decade. the existing drugs, which are known for their adverse reaction or toxicity, if utilized and allowed in different formulation, the new effective formulation may be discovered and developed. this may help in reducing various side effects, time and costs. in this study, fungal infection was inoculated superficially over the skin of guinea pigs and treated with the broad-spectrum antimicrobial (gatifloxacin) in comb ...	2014	24894182
biopesticides from plants: calceolaria integrifolia s.l.	the effects of persistent organic pollutants (pops) on humans and biodiversity are multiple and varied. nowadays environmentally-friendly pesticides are strongly preferred to pops. it is noteworthy that the crop protection role of pesticides and other techniques, i.e. biopesticides, plant extracts, prevention methods, organic methods, evaluation of plant resistance to certain pests under an integrated pest management (ipm), could improve the risks and benefits which must be assessed on a sound s ...	2014	24893349
preliminary study of the fungal ecology at the haematology and medical-oncology ward in bamako, mali.	data on fungal epidemiology in sub-saharan african countries are scarce. this exploratory study aimed to characterize the fungal flora at the onco-haematology ward of the national teaching hospital of point g in bamako, mali. a cross-sectional survey was conducted in the dry and in the rainy seasons. nasal swab and sputum samples were collected from the hospitalized patients while airborne fungal spores were collected using electrostatic dust-fall collectors. fungi were identified by their morph ...	2014	24889723
expression and evaluation of enzymes required for the hydrolysis of galactomannan.	the cost-effective production of bioethanol from lignocellulose requires the complete conversion of plant biomass, which contains up to 30 % mannan. to ensure utilisation of galactomannan during consolidated bioprocessing, heterologous production of mannan-degrading enzymes in fungal hosts was explored. the aspergillus aculeatus endo-β-mannanase (man1) and talaromyces emersonii α-galactosidase (agal) genes were expressed in saccharomyces cerevisiae y294, and the aspergillus niger β-mannosidase ( ...	2014	24888762
expression of aspergillus niger ia-001 endo-β-1,4-xylanase in pichia pastoris and analysis of the enzymic characterization.	the xylanaseb (xynb) (jx560731.1) gene of aspergillus niger ia-001 was optimized according to the codon usage of pichia pastoris and expressed in p. pastoris gs115. the optimized xynb expression level was increased 2.8 times relative to that of the wild-type xynb, and the dual-copy xynb (optimized) expression level was increased 1.9 times relative to that of the single-copy xynb (optimized). the activity of the dual-copy xynb ((xynb-opt)2) was maximized at 15,158.23 ± 45.11 u/ml after 120 h of s ...	2014	24888408
antifungal activity and identification of lactobacilli, isolated from traditional dairy product "katak".	filamentous moulds are the main spoilage microorganisms, responsible for significant economic losses and several healthy risks in human food chain. the lactic acid bacteria (lab), especially lactobacilli could be a natural antagonist of these dangerous organisms. in bulgaria, a very limited data exists on the antifungal activity of lab microbiota of fermented dairy products. in the present study, four active strains were isolated from traditional fermented curd/yogurt-like product "katak", produ ...	2014	24887637
phyllanthus wightianus müll. arg.: a potential source for natural antimicrobial agents.	phyllanthus wightianus belongs to euphorbiaceae family having ethnobotanical importance. the present study deals with validating the antimicrobial potential of solvent leaf extracts of p. wightianus. 11 human bacterial pathogens (bacillus subtilis, streptococcus pneumoniae, staphylococcus epidermidis, proteus vulgaris, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhimurium, escherichia coli, shigella flexneri, proteus vulgaris, and serratia marcescens) and 4 fungal pathogens (cand ...	2014	24883301
chitosan nanocomposite films based on ag-np and au-np biosynthesis by bacillus subtilis as packaging materials.	chitosan-silver (cs-ag) and chitosan-gold (cs-au) nanocomposites films were synthesized by a simple chemical method. a local bacterial isolate identified as bacillus subtilis ss subtilis was found to be capable to synthesize both silver nanoparticles (ag-np) and gold nanoparticles (au-np) from silver nitrate (agno3) and chloroauric acid (aucl(4-)) solutions, respectively. the biosynthesis of both ag-np and au-np characterize using uv/vis spectroscopy, scanning electron microscopy (sem), transmis ...	2014	24875320
antifungal efficiency assessment of the tio2 coating on façade paints.	the work studies the photocatalytic activity and the antifungal efficiency of the tio2/zn-al coatings placed on the target commercial façade paints. the photocatalytic active nanocomposite based on tio2 and zn-al-layered double hydroxides (znal-ldhs) was synthesized by a wet impregnation technique with 3 % w/w tio2. the freshly prepared suspension was applied by spray technique on the surfaces of the white façade paints. the goal of the work was to develop a method that quickly quantifies the an ...	2014	24875311
ochratoxin a and human health risk: a review of the evidence.	ochratoxin a (ota) is a mycotoxin produced by several fungal species including aspergillus ochraceus, a. carbonarius, a. niger, and penicillium verrucosum. ota causes nephrotoxicity and renal tumors in a variety of animal species; however, human health effects are less well-characterized. various studies have linked ota exposure with the human diseases balkan endemic nephropathy (ben) and chronic interstitial nephropathy (cin), as well as other renal diseases. this study reviews the epidemiologi ...	2015	24874522
altering the activation mechanism in thermomyces lanuginosus lipase.	it is shown by rational site-directed mutagenesis of the lid region in thermomyces lanuginosus lipase that it is possible to generate lipase variants with attractive features, e.g., high lipase activity, fast activation at the lipid interface, ability to act on water-soluble substrates, and enhanced calcium independence. the rational design was based on the lid residue composition in aspergillus niger ferulic acid esterase (faea). five constructs included lipase variants containing the full faea ...	2014	24870718
production of cellulosic ethanol and enzyme from waste fiber sludge using ssf, recycling of hydrolytic enzymes and yeast, and recombinant cellulase-producing aspergillus niger.	bioethanol and enzymes were produced from fiber sludges through sequential microbial cultivations. after a first simultaneous saccharification and fermentation (ssf) with yeast, the bioethanol concentrations of sulfate and sulfite fiber sludges were 45.6 and 64.7 g/l, respectively. the second ssf, which included fresh fiber sludges and recycled yeast and enzymes from the first ssf, resulted in ethanol concentrations of 38.3 g/l for sulfate fiber sludge and 24.4 g/l for sulfite fiber sludge. aspe ...	2014	24862324
biocontrol activity of the local strain of metschnikowia pulcherrima on different postharvest pathogens.	the strains of the yeast metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. biocontrol activity of m. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. biocontrol activity of pulcherrimin producing strain, m. pulcherrima umy15, isolated from local vineyards, was tested on different molds that cause food spoilage. m. pulcherrima umy15 was a very effective biocontrol agent against penicillium roqueforti, p. italicum, p. expansum, and ...	2014	24860671
effect of serum components on biofilm formation by aspergillus fumigatus and other aspergillus species.	biofilm production by microorganisms is critical for their pathogenicity. serum promotes biofilm production by aspergillus fumigatus; however, its effects on other aspergillus spp. have not been reported. we analyzed biofilm formation by five aspergillus spp., i.e., a. fumigatus, a. flavus, a. nidulans, a. niger, and a. terreus, and examined the effects of serum/serum proteins such as fetal bovine serum (fbs), fetuin a, and bovine serum albumin (bsa) on hyphal growth, hyphal branching, and extra ...	2014	24858605
high-level expression of aspergillus niger b-galactosidase in ashbya gossypii.	ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. the production levels achieved thus far were similar to those obtained with saccharomyces cerevisiae for the same proteins. here, the b-galactosidase from aspergillus niger was successfully expressed and secreted by a. gossypii from 2-mm plasmids carrying the native signal sequence at higher levels than those secreted by s. cerevisiae laboratorial strains. four different constitutive promoters were ...	2014	24851247
comparative analysis of pathogenic organisms in cockroaches from different community settings in edo state, nigeria.	cockroaches are abundant in nigeria and are seen to harbour an array of pathogens. environmental and sanitary conditions associated with demographic/socio-economic settings of an area could contribute to the prevalence of disease pathogens in cockroaches. a total of 246 cockroaches (periplaneta americana) in urban (benin, n=91), semi-urban (ekpoma, n=75) and rural (emuhi, n=70) settings in edo state, nigeria were collected within and around households. the external body surfaces and alimentary c ...	2014	24850961
purification and biochemical characterisation of glucoamylase from a newly isolated aspergillus niger: relation to starch processing.	herein, we investigate a glucoamylase from newly isolated aspergillus niger. the enzyme was purified, using fractionation, followed by anion-exchange chromatography and then characterised. the molecular mass of the enzyme was estimated to be ∼62,000da, using sds-page and 57151da, based on mass spectrometry results. the pi of the protein, and optimum ph/temperature of enzyme activity were 4.4, 5 and 70°c, respectively and the kinetic parameters (km, vmax and kcat) were determined to be 0.33 (mgml ...	2014	24837950
two-dimensional pca highlights the differentiated antitumor and antimicrobial activity of methanolic and aqueous extracts of laurus nobilis l. from different origins.	natural matrices are important sources of new antitumor and antimicrobial compounds. species such as laurus nobilis l. (laurel) might be used for this purpose, considering its medicinal properties. herein, in vitro activity against human tumor cell lines, bacteria, and fungi was evaluated in enriched phenolic extracts. specifically, methanol and aqueous extracts of wild and cultivated samples of l. nobilis were compared considering different phenolic groups. principal component analysis (pca) wa ...	2014	24826380
the effects of paenibacillus polymyxa e681 on antifungal and crack remediation of cement paste.	this study investigated the antifungal effects of cement paste containing paenibacillus polymyxa e681 against aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. to test the antifungal effects, cement paste containing p. polymyxa e681 was neutralized by co2 gas, and the fungal growth inhibition was examined according to the clear zone around the cement specimen. in addition to the antifungal effects of the cement paste added with bacteria, calcium crystal p ...	2014	24824950
asterisulphoxide and asterisulphone: two new antibacterial and antifungal metabolites from the tunisian asteriscus maritimus (l.) less.	two new sulphur-containing metabolites, asterisulphoxide 1 and asterisulphone 2, together with six known compounds, coniferaldehyde 4, 4-hydroxy-2-methoxybenzaldehyde 3, methylcaffeate 5, isobutyrate 10-isobutyryloxy-8,9-epoxythymyle 6, 8,9-dihydroxy-10-isobutyryloxythymol 7 and 8-hydroxy-9,14-diisobutyryloxythymol 8, were isolated from asteriscus maritimus roots. their structures were elucidated on the basis of spectroscopic evidence and comparison with authentic samples. compounds 1-8 were ass ...	2014	24824823
[increasing activity of a monoamine oxidase by random mutation].	the monoamine oxidase mutant a-1 (f210v/l213c) from aspergillus niger showed some catalytic activity on mexiletine. to futher improve its activity, the mutant was subjected to directed evolution with megawhop pcr (megaprimer pcr of whole plasmid) and selection employing a high-throughput agar plate-based colorimetric screen. this approach led to the identification of a mutant ep-1, which specific activity was 189% of that for a-1. the ep-1 also showed significantly improved enantioselectivity, w ...	2014	24818485
terrein biosynthesis in aspergillus terreus and its impact on phytotoxicity.	terrein is a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities. although it is produced by aspergillus terreus as one of its major secondary metabolites, not much is known about its biosynthetic pathway. here, we describe an unexpected discovery of the terrein biosynthesis gene locus made while we were looking for a pks gene involved in production of conidia coloration pigments common for aspergilli. the gene, ateg_00145, here named tera, is essent ...	2014	24816227
production of an endoinulinase from aspergillus niger aumc 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme.	two different substrates, sunflower (helianthus annuus l.) tubers and lettuce (lactuca sativa) roots, were tested. using a mixture of both wastes resulted in higher production of endoinulinase than either waste alone. also, ten fungal species grown on these substrates as inexpensive, carbon sources were screened for the best production of endoinulinase activities. of these, aspergillus niger aumc 9375 was the most productive, when grown on the mixture using a 6:1 w/w ratio of sun flower: lettuce ...	2014	24810318