Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| crop yields and nematode population densities in triticale-cotton and triticale-soybean rotations. | triticale cv. beagle 82, cotton cv. mcnair 235, and soybean cv. twiggs were arranged in three cropping sequences to determine the effects of fenamiphos and cropping sequence on nematode population densities and crop yields under conservation tillage for 4 years. the cropping sequences were triticale (t)-cotton (c)-t-c, t-soybean (s)-t-s, and t-c-t-s. numbers of meloidogyne incognita second-stage juveniles declined on trificale but increased on cotton and soybean each year. root-gall indices of c ... | 1998 | 19274228 |
| peanut-cotton-rye rotations and soil chemical treatment for managing nematodes and thrips. | in the southeastern united states, a cotton-peanut rotation is attractive because of the high value and extensive planting of both crops in the region. the objective of this experiment was to determine the effects of cotton-peanut rotations, rye, and soil chemical treatments on management of plant-parasitic nematodes, thrips, and soilborne fungal diseases and on crop yield. peanut-cotton-rye rotations were conducted from 1988 to 1994 on tifton loamy sand (plinthic kandiudult) infested primarily ... | 1998 | 19274213 |
| evaluation of nemx, a new cultivar of cotton with high resistance to meloidogyne incognita. | the level of resistance to root-knot nematode, meloidogyne incognita, in nemx, a new cultivar of the acala-type upland cotton, was evaluated in relation to four resistant breeding lines (n6072, n8577, n901, and n903) and four susceptible cultivars (maxxa, sj2, royale, and prema). in growth pouch tests, an average of only 4 nematode egg masses was produced on roots of nemx or the resistant lines, compared to a significantly higher average of 21 on the susceptible cultivars. in pot tests, the nema ... | 1997 | 19274190 |
| tolerance to rotylenchulus reniformis and resistance to meloidogyne incognita race 3 in high-yielding breeding lines of upland cotton. | field experiments in 1992 and 1994 were conducted to determine the effect of rotylenchulus reniformis, reniform nematode, on lint yield and fiber quality of 10 experimental breeding lines of cotton (gossypium hirsutum) in untreated plots or plots fumigated with 1,3-dichloropropene. controls were la. rn 1032, a germplasm line possessing some resistance to r. reniformis, and stoneville 453, a cultivar that is susceptible to reniform nematode. several breeding lines produced greater lint yields tha ... | 1997 | 19274165 |
| efficacy of cotton root destruction and winter cover crops for suppression of hoplolaimus columbus. | the efficacy of rye (secale cereale) and wheat (triticum aestivum) winter cover crops and cotton stalk and root destruction (i.e., pulling them up) were evaluated in field tests during two growing seasons for hoplolaimus columbus management in cotton. the effect of removing debris from the field following root destruction also was evaluated. wheat and rye produced similar amounts of biomass, and both crops produced more biomass (p </= 0.05) following cotton root destruction. cover crops did not ... | 2000 | 19271009 |
| effect of foliar applications of oxamyl with aldicarb for the management of rotylenchulus reniformison cotton. | the efficacy of foliar applications of oxamyl were evaluated for the management of rotylenchulus reniformis on cotton in mississippi. two tests were established in tallahatchie county on a fine sandy loam soil (56.8% sand, 37.8% silt, 5.3% clay, ph 5.4, and 0.3% om) naturally infested with r. reniformis. oxamyl was applied as a foliar spray at 0.14, 0.27, or 0.53 kg a.i./ha to cotton plants that had reached the sixth true leaf growth stage. a second oxamyl application was applied 14 days after t ... | 2000 | 19271008 |
| tolerance of selected cotton lines to rotylenchulus reniformis. | the reproductive and damage potential of the reniform nematode, rotylenchulus reniformis, on five cotton breeding lines reported as tolerant to this nematode in texas were compared with two standard cotton cultivars, deltapine 50 and stoneville la 887, in a north carolina field naturally infested with r. reniformis. numbers of r. reniformis in soil were suppressed at mid-season, and cotton-lint yield was increased by preplant fumigation with 1,3-dichloropropene. population densities of r. renifo ... | 2000 | 19271004 |
| root galling and reproduction of meloidogyne incognita isolates from texas on resistant cotton genotypes. | several cotton genotypes with resistance to meloidogyne incognita have been released in recent years. to estimate the durability of this resistance, galling severity on these resistant genotypes by m. incognita was measured. nematode isolates (115 total) were collected from cotton fields in 14 texas counties in august and september 1996 and 1997. four additional isolates from maryland, mississippi, and north carolina were also tested. the isolates were evaluated in 12 greenhouse experiments for ... | 2000 | 19271003 |
| a survey of plant-parasitic nematodes associated with cotton in northeastern louisiana. | a survey was conducted in northeastern louisiana to determine the frequency and abundance of plant-parasitic nematodes associated with cotton. in fall 1997 and 1998, more than 600 soil samples were collected from cotton fields representing 6,200 ha, which is 5.3% of the cotton production hectarage in this region. composite soil samples were collected from 10 ha in each field. nematodes were extracted by gravity screening and sucrose centrifugation, identified to genus, and quantified. nine gener ... | 2000 | 19271002 |
| effects of potato-cotton cropping systems and nematicides on plant-parasitic nematodes and crop yields. | belonolaimus longicaudatus has been reported as damaging both potato (solanum tuberosum) and cotton (gossypium hirsutum). these crops are not normally grown in cropping systems together in areas where the soil is infested with b. longicaudatus. during the 1990s cotton was grown in a potato production region that was a suitable habitat for b. longicaudatus. it was not known how integrating the production of these two crops by rotation or double-cropping would affect the population densities of b. ... | 2000 | 19270980 |
| population dynamics of belonolaimus longicaudatusin a cotton production system. | belonolaimus longicaudatus is a recognized pathogen of cotton (gossypium hirsutum), but insufficient information is available on the population dynamics and economic thresholds of b. longicaudatus in cotton production. in this study, data collected from a field in florida were used to develop models predicting population increases of b. longicaudatus on cotton and population declines under clean fallow. population densities of b. longicaudatus increased on cotton, reaching a carrying capacity of ... | 2000 | 19270968 |
| yield reduction and root damage to cotton induced by belonolaimus longicaudatus. | sting nematode (belonolaimus longicaudatus) is recognized as a pathogen of cotton (gossypium hirsutum), but the expected damage from a given population density of this nematode has not been determined. the objective of this study was to quantify the effects of increasing initial population densities (pi) of b. longicaudatus on cotton yield and root mass. in a field plot study, nematicide application and cropping history were used to obtain a wide range of pi values. cotton yields were regressed ... | 2000 | 19270967 |
| population dynamics of meloidogyne incognita, m. arenaria,and other nematodes and crop yields in rotations of cotton, peanut, and wheat under minimum tillage. | wheat, cotton, and peanut were arranged in three cropping sequences to determine the effects of fenamiphos (6.7 kg a.i./ha) and cropping sequence on nematode population densities and crop yields under conservation tillage and irrigation for 6 years. the cropping sequences included a wheat winter cover crop each year and summer crops of cotton every year, peanut every year, or cotton rotated every other year with peanut. the population densities of meloidogyne spp. and helicotylenchus dihystera w ... | 2000 | 19270949 |
| comparison of variable and single-rate applications of aldicarb on cotton yield in fields infested with meloidogyne incognita. | variable-rate applications of the nematicide aldicarb were compared to producer standard rates in eight field tests over 3 years. test areas (308 to 1,015 m long) were divided into eight or five blocks. each block contained two plots with a variable-rate treatment (vrt) of aldicarb and a producer standard treatment (pst) of aldicarb. each vrt plot was divided into three subunits and intensively sampled for meloidogyne incognita in either the fall or spring before planting. rates of aldicarb were ... | 1999 | 19270939 |
| survey of crop losses in response to phytoparasitic nematodes in the united states for 1994. | previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ... | 1999 | 19270925 |
| bahiagrass, corn, cotton rotations, and pesticides for managing nematodes, diseases, and insects on peanut. | florunner peanut was grown after 1 and 2 years of tifton 9 bahiagrass, corn, cotton, and continuous peanut as whole-plots. pesticide treatments aldicarb (3.4 kg a.i./ha), flutolanil (1.7 kg a.i./ha), aldicarb + flutolanil, and untreated (control) were sub-plots. numbers of meloidogyne arenaria second-stage juveniles in the soil and root-gall indices of peanut at harvest were consistently lower in plots treated with aldicarb and aldicarb + flutolanil than in flutolanil-treated and untreated plots ... | 1999 | 19270889 |
| impact of cotton production systems on management of hoplolaimus columbus. | the effectiveness of selected cultural practices in managing the columbia lance nematode, hoplolaimus columbus, on cotton was evaluated in experiments in growers' infested fields. the effects of planting date, cotton cultivar, treatment with the growth regulator mepiquat chloride, and destruction of cotton-root systems after harvest on cotton-lint yield and population densities of h. columbus were studied. the yield of cotton cultivar deltapine 50 was negatively related (p = 0.054) to initial po ... | 2003 | 19265977 |
| rotylenchulus reniformis management in cotton with crop rotation. | one-year crop rotations with corn or highly resistant soybean were evaluated at four locations for their effect on rotylenchulus reniformis population levels and yield of a subsequent cotton crop. four nematicide (aldicarb) regimes were included at two of the locations, and rotation with reniform-susceptible soybean was included at the other two locations. one-year rotations to corn or resistant soybean resulted in lower r. reniformis population levels (p </= 0.05) than those found in cotton at ... | 2003 | 19265975 |
| effects of site-specific application of aldicarb on cotton in a meloidogyne incognita-infested field. | cotton farmers in missouri commonly apply a single rate of aldicarb throughout the field at planting to protect their crop from meloidogyne incognita, even though these nematodes are spatially aggregated. our purpose was to determine the effect of site-specific application of aldicarb on cotton production in a field infested with these nematodes in 1997 and 1998. cotton yields were collected from sites not treated with aldicarb (control), sites receiving aldicarb at the standard recommended rate ... | 2002 | 19265917 |
| management of root-knot and reniform nematodes in ultra-narrow row cotton with 1,3-dichloropropene. | ultra-narrow row cotton studies were conducted during 1999 at two field sites in northern florida. one site was naturally infested with meloidogyne incognita race 3 and the other with rotylenchulus reniformis. the fumigant 1,3-dichloropropene (1,3-d) was applied broadcast at rates of 0, 16, 32, 48, 64, 80, and 96 kg ai./ha in replicated plots before planting delta pine 655 brr cotton in 25-cm-wide rows. post-harvest soil population densities at the root-knot nematode site had a significant (p </ ... | 2001 | 19265894 |
| histological observations of rotylenchulus reniformis on gossypium longicalyx and interspecific cotton hybrids. | observations on the development of reniform nematode (rotylenchulus reniformis) on roots of gossypium longicalyx, g. hirsutum, and two interspecific hybrids derived from them were made by light microscopy. gossypium longicalyx is reported to be immune to reniform nematode, but the mechanism(s) for resistance are unknown. penetration of g. longicalyx roots by female nematodes was confirmed, and incipient swelling of the females, indicating initiation of maturation of the reproductive system, was ... | 2005 | 19262889 |
| rotylenchulus reniformis below plow depth suppresses cotton yield and root growth. | damage to cotton by rotylenchulus reniformis below plow depth was evaluated in a sandy clay loam soil at weslaco, texas. in december 1999, 14 holes on 51-cm centers were dug 91 cm deep along the planting bed and adjacent furrow and 2 ml of 1,3-dichloropropene was placed 91, 61, and 30 cm deep as each hole was refilled and packed. this technique eliminated 96%, 81%, and 74% of r. reniformis down to 107 cm at distances 0, 25, and 51 cm laterally from the point of application (p </= 0.05), whereas ... | 2005 | 19262875 |
| vertical distribution of rotylenchulus reniformis in cotton fields. | the possible impact of rotylenchulus reniformis below plow depth was evaluated by measuring the vertical distribution of r. reniformis and soil texture in 20 symptomatic fields on 17 farms across six states. the mean nematode population density per field, 0 to 122 cm deep, ranged from 0.4 to 63 nematodes/g soil, and in 15 fields more than half of the r. reniformis present were below 30.5 cm, which is the greatest depth usually plowed by farmers or sampled by consultants. in 11 fields measured, r ... | 2005 | 19262871 |
| reproduction of belonolaimus longicaudatus, meloidogyne javanica, paratrichodorus minor, and pratylenchus brachyurus on pearl millet (pennisetum glaucum). | pearl millet (pennisetum glaucum) has potential as a grain crop for dryland crop production in the southeastern united states. whether or not pearl millet will be compatible in rotation with cotton (gossypium hirsutum), corn (zea mays), and peanut (arachis hypogaea) will depend, in part, on its host status for important plant-parasitic nematodes of these crops. the pearl millet hybrid 'tifgrain 102' is resistant to both meloidogyne incognita race 3 and m. arenaria race 1; however, its host statu ... | 2005 | 19262863 |
| histological changes in gossypium hirsutum associated with reduced reproduction of rotylenchulus reniformis. | the reniform nematode (rotylenchulus reniformis) is an important parasite of upland cotton (gossypium hirsutum). parasitism involves the formation of syncytia to provide nutrition for the female. events that occur at the feeding site may determine the degree of susceptibility of cotton plants to reniform nematode. the objective of this work was to describe histological modifications associated with reduced reproduction of rotylenchulus reniformis in upland cotton roots. 'deltapine 50' cotton and ... | 2005 | 19262859 |
| influence of poultry litter applications on nematode communities in cotton agroecosystems. | the effects of the application of poultry litter at 0.0, 6.7, 13.4, and 20.1 tons/ha on population changes during the growing season on nematode communities were evaluated in two cotton production fields in north carolina. numbers of bactivorous nematodes increased at midseason in response to the rate at which litter was applied but decreased with increasing litter application rates at cotton harvest. numbers of fungivores at cotton harvest were related positively to the rate of litter applied, ... | 2004 | 19262834 |
| suppression of rotylenchulus reniformis on cotton by the nematophagous fungus arf. | the reniform nematode, rotylenchulus reniformis linford &oliveira, has become a serious threat to cotton (gossypium hirsutum l.) production in the united states during the past decade. the objective of this study is to isolate fungi from eggs of r. reniformis and select potential biological control agents for r. reniformis on cotton. soil samples were collected from cotton fields located in jefferson county, arkansas. eight genera of fungi were included in the 128 fungal isolates obtained, and a ... | 2004 | 19262806 |
| interaction of rotylenchulus reniformis with seedling disease pathogens of cotton. | the impact of 10 fusarium species in concomitant association with rotylenchulus reniformis on cotton seedling disease was examined under greenhouse conditions. in experiment 1, fungal treatments consisted of fusarium chlamydosporum, f. equiseti, f. lateritium, f. moniliforme, f. oxysporum, f. oxysporum f.sp. vasinfectum, f. proliferatum, f. semitectum, f. solani, and f. sporotrichioides; rhizoctonia solani; and thielaviopsis basicola. the experimental design was a 2 x 14 factorial consisting of ... | 2004 | 19262802 |
| competition of meloidogyne incognita and rotylenchulus reniformis on cotton following separate and concomitant inoculations. | it has been hypothesized rotylenchulus reniformis (rr) has a competitive advantage over meloidogyne incognita (mi) in the southeastern cotton production region of the united states. this study examines the reproduction and development of meloidogyne incognita (mi) and rotylenchulus reniformis (rr) in separate and concomitant infections on cotton. under greenhouse conditions, cotton seedlings were inoculated simultaneously with juveniles (j2) of m. incognita and vermiform adults of r. reniformis ... | 2003 | 19262774 |
| relationships between tolerance and resistance to meloidogyne incognita in cotton. | the southern root-knot nematode, meloidogyne incognita, is the most damaging pathogen of cotton in the united states, and both resistance and tolerance to m. incognita could be valuable management approaches. our objectives were to evaluate advanced cotton breeding lines for resistance and tolerance to m. incognita and to determine if a relationship between resistance and tolerance exists. reproduction of m. incognita was evaluated on 17 breeding lines, a susceptible control (delta and pine land ... | 2003 | 19262772 |
| detection of suppressiveness against rotylenchulus reniformis in soil from cotton (gossypium hirsutum) fields in texas and louisiana. | rotylenchulus reniformis is a major problem confronting cotton production in the central part of the cotton belt of the united states of america. in this study, the hypothesis that natural antagonists in some cases are responsible for unusually low densities of the nematode in certain fields was tested by assaying soils from 22 selected fields for the presence of transferable agents in pots containing cotton plants. in one field, soil from four different depth ranges was tested. in the first of ... | 2008 | 19259517 |
| phenotypic expression of rkn1-mediated meloidogyne incognita resistance in gossypium hirsutum populations. | the root-knot nematode meloidogyne incognita is a damaging pest of cotton (gossypium hirsutum) worldwide. a major gene (rkn1) conferring resistance to m. incognita was previously identified on linkage group a03 in g. hirsutum cv. acala nemx. to determine the patterns of segregation and phenotypic expression of rkn1, f(1), f(2), f(2:3), bc(1)f(1) and f(2:7) recombinant inbred lines (ril) from intraspecific crosses between acala nemx and a closely related susceptible cultivar acala sj-2 were inocu ... | 2006 | 19259455 |
| reproduction of meloidogyne incognita on winter cover crops used in cotton production. | substantial reproduction of meloidogyne incognita on winter cover crops may lead to damaging populations in a subsequent cotton (gossypium hirsutum) crop. the amount of population increase during the winter depends on soil temperature and the host status of the cover crop. our objectives were to quantify m. incognita race 3 reproduction on rye (secale cereale) and several leguminous cover crops and to determine if these cover crops increase population densities of m. incognita and subsequent dam ... | 2006 | 19259434 |
| bacillus thuringiensis cry1ac resistance frequency in tobacco budworm (lepidoptera: noctuidae). | the tobacco budworm, heliothis virescens (f.) (lepidoptera: noctuidae), is one of the most important pests of cotton, gossypium hirsutum l, that has become resistant to a wide range of synthetic insecticides. crylac-expressing cotton has proven its effectiveness against this insect since its introduction in north america in 1996. however, the constant exposure of tobacco budworm to this protein toxin may result in the development of resistance to it. to estimate the frequency of alleles that con ... | 2009 | 19253658 |
| can the amount of corn acreage predict fall armyworm (lepidoptera: noctuidae) infestation levels in nearby cotton? | fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae), is a major pest of corn, zea mays l., and a significant, but more sporadic, pest of cotton, gossypium hirsutum l., in the western hemisphere. previous studies showed that the cotton infestations primarily involve a fall armyworm subpopulation known as the "corn-strain" for which corn is the preferred host plant. it was suggested that the fall armyworm infesting cotton originated in corn and spread into secondary hosts as ... | 2009 | 19253639 |
| comparisons of boll weevil (coleoptera: curculionidae) pheromone traps with and without kill strips. | boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), eradication programs typically equip pheromone traps with an insecticide-impregnated kill strip. these strips are intended to kill captured insects, thereby simplifying trap servicing and reducing the loss of weevils from predation and escape. however, the effectiveness of kill strips has not been extensively evaluated. we examined the influences of kill strips on weevil captures, trap servicing, and the incidences of w ... | 2009 | 19253635 |
| temporal analysis of cotton boll symptoms resulting from southern green stink bug feeding and transmission of a bacterial pathogen. | the southern green stink bug, nezara viridula (l.), is a significant pest of cotton, gossypium hirsutum l., and is becoming an increasing challenge due to the decrease in use of broad-spectrum insecticides on the crop. the southern green stink bug can vector an opportunistic pantoea agglomerans strain (designated sc 1-r) into cotton bolls, resulting in infection. the appearance of stink bug damage varies, and pest managers cannot readily identify its source. this research reports a systematic de ... | 2009 | 19253615 |
| identification and expression of a new delta-12 fatty acid desaturase (fad2-4) gene in upland cotton and its functional expression in yeast and arabidopsis thaliana plants. | a cotton (gossypium hirsutum l.) genomic clone encompassing a 17.9-kb dna fragment was found to contain a delta-12 fatty acid desaturase gene (designated fad2-4). the fad2-4 open reading frame has 1,155bp and is uninterrupted, encoding a conceptual fad2-4 polypeptide of 384 amino acids that has 98% identity with the cotton fad2-3 polypeptide. the fad2-4 gene has a single intron of 2,780 bp in its 5'-untranslated region (5'-utr). the 3'-flanking regions and 5'-utr introns in the fad2-4 and fad2-3 ... | 2009 | 19217793 |
| scanning electron microscopy applied to seed-borne fungi examination. | the aim of this study was to test the standard scanning electron microscopy (sem) as a potential alternative to study seed-borne fungi in seeds, by two different conditions of blotter test and water restriction treatment. in the blotter test, seeds were subjected to conditions that enabled pathogen growth and expression, whereas the water restriction method consisted in preventing seed germination during the incubation period, resulting in the artificial inoculation of fungi. in the first condit ... | 2009 | 19204924 |
| undamaged cotton plants yield more if their neighbour is damaged: implications for pest management. | understanding the compensatory responses of crops to pest damage is important in developing pest thresholds. compensation for pest damage in crops can occur at the plant level, where the architecture, growth dynamics and allocation patterns of damaged plants are altered, allowing them to recover or, at the crop level, where differential damage between plants may alter plant-to-plant interactions. we investigated growth and yield of cotton (gossypium hirsutum l.) following non-uniform manual defo ... | 2009 | 19203400 |
| devitalization of transgenic seed that preserves dna and protein integrity. | agricultural biotechnology companies have been asked to provide intact transgenic seed to regulatory agencies as reference materials for evaluating transgene and protein detection methods (pcr and immunoassay). due to intellectual-property and product-stewardship considerations, submission of devitalized seed prior to regulatory approval is preferable in any given country. commonly used devitalization procedures, such as heating or autoclaving, degrade the protein and/or dna rendering the seed u ... | 2008 | 19183799 |
| construction of a bacterial artificial chromosome library of tm-1, a standard line for genetics and genomics in upland cotton. | a bacterial artificial chromosome (bac) library was constructed for gossypium hirsutum acc. tm-1, a genetic and genomic standard line for upland cotton. the library consists of 147 456 clones with an average insert size of 122.8 kb ranging from 97 to 240 kb. about 96.0% of the clones have inserts over 100 kb. therefore, this library represents theoretically 7.4 haploid genome equivalents based on an ad genome size of 2 425 mb. clones were stored in 384 384(-) well plates and arrayed into multipl ... | 2009 | 19166501 |
| index selection on seed traits under direct, cytoplasmic and maternal effects in multiple environments. | crop seeds are important sources of protein, oil, and carbohydrates for food, animal feeds, and industrial products. recently, much attention has been paid to quality and functional properties of crop seeds. however, seed traits possess some distinct genetic characteristics in comparison with plant traits, which increase the difficulty of genetically improving these traits. in this study, diallel analysis for seed models with genotype by environment interaction (ge) effect was applied to estimat ... | 2009 | 19161944 |
| expression of genes associated with carbohydrate metabolism in cotton stems and roots. | cotton (gossypium hirsutum l) is an important crop worldwide that provides fiber for the textile industry. cotton is a perennial plant that stores starch in stems and roots to provide carbohydrates for growth in subsequent seasons. domesticated cotton makes these reserves available to developing seeds which impacts seed yield. the goals of these analyses were to identify genes and physiological pathways that establish cotton stems and roots as physiological sinks and investigate the role these p ... | 2009 | 19161628 |
| lethal and sublethal effects of endosulfan on apolygus lucorum (hemiptera: miridae). | various pesticides directly impact insect pest populations through acute toxicity (lethal) effects, but they also cause a range of indirect (sublethal) effects on pest physiology or behavior. in this study, we describe both lethal and sublethal effects of endosulfan on the survival, development, and fecundity of apolygus lucorum meyer-dür (hemiptera: miridae), an important pest of bacillus thuringiensis (bt) cotton, gossypium hirsutum l., in china. we quantify pesticide effects on adult mirids a ... | 2008 | 19133460 |
| overexpression of thellungiella halophila h(+)-ppase (tsvp) in cotton enhances drought stress resistance of plants. | an h(+)-ppase gene, tsvp from thellungiella halophila, was transferred into two cotton (gossypium hirsutum) varieties (lumianyan19 and lumianyan 21) and southern and northern blotting analysis showed the foreign gene was integrated into the cotton genome and expressed. the measurement of isolated vacuolar membrane vesicles demonstrated that the transgenic plants had higher v-h(+)-ppase activity compared with wild-type plants (wt). overexpressing tsvp in cotton improved shoot and root growth, and ... | 2009 | 19130078 |
| total and percent atropisomers of gossypol and gossypol-6-methyl ether in seeds from pima cottons and accessions of gossypium barbadense l. | gossypol occurs naturally in the seed, foliage, and roots of the cotton plant ( gossypium ) as atropisomers due to restricted rotation around the binaphthyl bond. the atropisomers differ in their biological activities. (-)-(r)-gossypol is more toxic and exhibits significantly greater anticancer activity than the (+)-(s)-atropisomer. most commercial upland ( gossypium hirsutum ) cottonseeds have an (r)- to (s)-gossypol ratio of approximately 2:3, but some pima ( gossypium barbadense ) seeds have ... | 2009 | 19113939 |
| analysis of cotton (gossypium hirsutum) root proteomes during a compatible interaction with the black root rot fungus thielaviopsis basicola. | a proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with thielaviopsis basicola. microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-de analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein exp ... | 2009 | 19105169 |
| [cotton fiber quality traits were controlled mainly by maternal plant genotype]. | hai1, a gossypium barbadense l. variety with super fiber quality, and ccri36 and zhong221, two upland cotton cultivars (gossypium hirsutum l.), were used as recurrent parents to develop two backcross combinations of ccri36xhai1 and zhong221xhai1. fiber quality of inter-crossing bolls and self-crossing bolls were analyzed from different generations of the two combinations. the results showed that there existed significant difference in the average value, pole difference and cv% of fiber quality t ... | 2008 | 19073557 |
| cotton metallothionein ghmt3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast. | a cdna clone encoding a 64-amino acid type 3 metallothionein protein, designated ghmt3a, was isolated from cotton (gossypium hirsutum) by cdna library screening. northern blot analysis indicated that mrna accumulation of ghmt3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also by heavy metal ions, abscisic acid (aba), ethylene, and reactive oxygen species (ros) in cotton seedlings. transgenic tobacco (nicotiana tabacum) plants overexpressing ghmt3a showe ... | 2009 | 19033550 |
| cotton plant, gossypium hirsutum l., defense in response to nitrogen fertilization. | plants respond to insect herbivory by producing dynamic changes in an array of defense-related volatile and nonvolatile secondary metabolites. a scaled response relative to herbivory levels and nutrient availability would be adaptive, particularly under nutrient-limited conditions, in minimizing the costs of expressed defensive pathways and synthesis. in this study, we investigated effects of varying nitrogen (n) fertilization (42, 112, 196, and 280 ppm n) on levels of cotton plant (gossypium hi ... | 2008 | 19020938 |
| a cotton (gossypium hirsutum) dre-binding transcription factor gene, ghdreb, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat. | a cotton (g. hirsutum l.) dehydration responsive element binding protein gene, ghdreb, which encodes a 153 amino acid protein containing a conserved ap2/erebp domain, was isolated from the cdna library of cotton cv. simian 3 by a yeast one-hybrid system. rna blot analysis showed that the ghdreb gene was induced in cotton seedlings by drought, high salt and cold stresses. an electrophoretic mobility shift assay (emsa) indicated that the ghdreb protein bound specifically to the dre core element (a ... | 2009 | 19005655 |
| [overexpression of spinacia oleracea betaine aldehyde dehydrogenase (sobadh) gene confers the salt and cold tolerant in gossypium hirsutum l]. | the open reading frame of spinacia oleracea betaine aldehyde dehydrogenase (sobadh) was retrieved from spinacia oleracea and inserted into the agrobacterium tumefaciens binary vector pbin438, which was driven by camv35s promoter, and produced the new binary vector pbsb. a. tumefaciens lba4404 carrying this plasmid was used in genetic transformation of plants. forty-five primary transgenic plants were detected by pcr and verified by the southern blotting from 65 regenerated plants, of which 27 tr ... | 2008 | 18998552 |
| expressions of three cotton genes encoding the pip proteins are regulated in root development and in response to stresses. | cotton (gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (summer). to investigate molecular mechanism of water regulation in cotton plants, three cdnas encoding the plasma membrane intrinsic protein (pip) were isolated from cotton root cdna library, and designated ghpip1;1, ghpip2;1 and ghpip2;2, respectively. all of the three pip proteins displayed water channel activity in xenopus laevis oocytes. ... | 2009 | 18956193 |
| cotton harvest at 40% versus 75% boll-splitting on yield and economic return under standard and proactive boll weevil (coleoptera: curculionidae) spray regimes. | the standard practice of two or three preemptive insecticide applications at the start of pinhead (1-2-mm-diameter) squaring followed by threshold-triggered (when 10% of randomly selected squares have oviposition punctures) insecticide applications for boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), control does not provide reliable protection of cotton, gossypium hirsutum l., lint production. this study, conducted during 2004 and 2005, showed that three to six fewer ... | 2008 | 18950042 |
| yield response of dual-toxin bt cotton to helicoverpa zea infestations. | field cage experiments were conducted to determine the impact of bollworms, helicoverpa zea (boddie), on yields of bollgard ii and widestrike cotton, gossypium hirsutum l. one-day-old bollworm larvae were infested in white flowers of bollgard ii and in white flowers and terminals of widestrike cotton. the infestation levels included 0, 50, and 100% of white flowers for each type of cotton. terminal infestations included one or two larvae per terminal on widestrike cotton. larvae were placed in f ... | 2008 | 18950041 |
| inoculum densities of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita in relation to the development of fusarium wilt and the phenology of cotton plants (gossypium hirsutum). | abstract development of fusarium wilt in upland cotton (gossypium hirsutum) usually requires infections of plants by both meloidogyne incognita and fusarium oxysporum f. sp. vasinfectum. in this study, the soil densities of m. incognita and f. oxysporum f. sp. vasinfectum and the incidence of fusarium wilt in three field sites were determined in 1982-1984. multiple regression analysis of percent incidence of fusarium wilt symptoms on population densities of m. incognita and f. oxysporum f. sp. v ... | 1997 | 18945178 |
| induction of terpenoid synthesis in cotton roots and control of rhizoctonia solani by seed treatment with trichoderma virens. | abstract research on the mechanisms employed by the biocontrol agent trichoderma virens to suppress cotton (gossypium hirsutum) seedling disease incited by rhizoctonia solani has shown that mycoparasitism and antibiotic production are not major contributors to successful biological control. in this study, we examined the possibility that seed treatment with t. virens stimulates defense responses, as indicated by the synthesis of terpenoids in cotton roots. we also examined the role of these terp ... | 2000 | 18944616 |
| isolation, selection, and efficacy of pochonia chlamydosporia for control of rotylenchulus reniformis on cotton. | abstract the reniform nematode, rotylenchulus reniformis, is a serious threat to cotton (gossypium hirsutum) production in the united states, causing an annual loss of about $80 million. the objective of this study was to isolate fungi from eggs of r. reniformis and select potential biocontrol agents for r. reniformis on cotton. we focused on the fungus pochonia chlamydosporia because it suppresses root-knot and cyst nematodes and because preliminary data indicated that it was present in arkansa ... | 2005 | 18944410 |
| evolution of virulence in fusarium oxysporum f. sp. vasinfectum using serial passage assays through susceptible cotton. | fifty strains of fusarium oxysporum, recovered from rhizosphere soil around native gossypium species and found to be mildly virulent on cotton (gossypium hirsutum), were used to assay the propensity for evolution of virulence using serial passage assays through cotton. only one lineage a strain, 2613, successfully completed 10 successive passages, while all others lost the ability to cause foliar disease symptoms at various stages during this process. based on 46 amplified fragment length polymo ... | 2008 | 18944080 |
| four near-isogenic lines of cotton with different genes for bacterial blight resistance. | abstract the development and genetic characterization of four near-isogenic lines (nils) of cotton (gossypium hirsutum) is described herein. each line contains a single, but different, gene for resistance to bacterial blight caused by xanthomonas campestris pv. malvacearum. the lines were derived using at least six backcrosses to the susceptible recurrent parent 'acala 44', followed by single plant-progeny row selection for uniformity. the nils are homozygous for the b(2), b(4), b(in), or b(7) g ... | 2002 | 18943887 |
| the ghacs1 gene encodes an acyl-coa synthetase which is essential for normal microsporogenesis in early anther development of cotton. | microsporogenesis, associated with the functional expression of many genes, is a highly programmed and regulated process in flowering plants. to elucidate the roles of genes during anther development, two anther-specific cdnas (designated ghacs1 and ghacs2) encoding acyl-coa synthetases (acss) were isolated from a cotton (gossypium hirsutum) flower cdna library. subsequently, the corresponding ghacs1 gene was isolated from a cotton genomic dna library. real-time quantitative rt-pcr and northern ... | 2009 | 18826432 |
| transcriptional profiling of mature arabidopsis trichomes reveals that noeck encodes the mixta-like transcriptional regulator myb106. | leaf hairs (trichomes) of arabidopsis (arabidopsis thaliana) have been extensively used as a model to address general questions in cell and developmental biology. here, we lay the foundation for a systems-level understanding of the biology of this model cell type by performing genome-wide gene expression analyses. we have identified 3,231 genes that are up-regulated in mature trichomes relative to leaves without trichomes, and we compared wild-type trichomes with two mutants, glabra3 and triptyc ... | 2008 | 18805951 |
| global analysis of gene expression in cotton fibers from wild and domesticated gossypium barbadense. | gossypium barbadense is widely cultivated because of its extra-long staple cotton with superior luster, silkiness and high yield. these economically important traits were selected during initial domestication of an agronomically inferior wild ancestor, followed by millennia of human-mediated selection. to reveal the effects of this history on the cotton fiber transcriptome, we conducted comparative expression profiling on mechanically isolated fiber cells at three different stages encompassing e ... | 2008 | 18803775 |
| molecular diversity and association mapping of fiber quality traits in exotic g. hirsutum l. germplasm. | the narrow genetic base of cultivated cotton germplasm is hindering the cotton productivity worldwide. although potential genetic diversity exists in gossypium genus, it is largely 'underutilized' due to photoperiodism and the lack of innovative tools to overcome such challenges. the application of linkage disequilibrium (ld)-based association mapping is an alternative powerful molecular tool to dissect and exploit the natural genetic diversity conserved within cotton germplasm collections, grea ... | 2008 | 18801424 |
| nectar feeding by wandering spiders on cotton plants. | spiders are assumed to be strictly carnivorous in assessments of their nutritional and energetic requirements, their habitat preferences, and their potential as biological control agents. however, members of salticidae (jumping spiders), thomisidae (crab spiders), and the fast-moving miturgidae, anyphaenidae, and corinnidae, all non-webbuilding wandering spiders, have been observed at floral and extrafloral nectaries of plants, presumably feeding on nectar. to test spiders in the field for necta ... | 2008 | 18801266 |
| purification and biochemical characterization of polyphenol oxidases from embryogenic and nonembryogenic cotton (gossypium hirsutum l.) cells. | polyphenol oxidases (ppos) were isolated from cell suspensions of two cultivars of cotton (gossypium hirsutum l.), and their biochemical characteristics were studied. ppo from coker 312, an embryogenic cultivar, showed a highest affinity to catechol 20 mm, and ppo from r405-2000, a nonembryogenic cultivar, showed a highest affinity to 4-methylcatechol 20 mm. the optimal ph for ppo activity was 7.0 and 6.0 for coker 312 and r405-2000, respectively. the enzyme had an optimal temperature of 25 degr ... | 2009 | 18795241 |
| analysis of xyloglucan endotransglycosylase/hydrolase (xth) genes from allotetraploid (gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation. | multiple cellular pathways have been shown to be involved during fiber initiation and elongation stages in the cultivated allotetraploid cotton (gossypium hirsutum). the cell wall enzymes xyloglucan endotransglycosylase/hydrolases (xth) have been reported to be associated with the biosynthesis of the cell wall and the growth of cotton fibers, probably regulating the plasticity of the primary cell wall. among various cotton fiber cdnas found to be preferentially expressed in cotton fibers, a xylo ... | 2009 | 18789555 |
| the r2r3 myb transcription factor ghmyb109 is required for cotton fiber development. | cotton (gossypium hirsutum l.) fibers are single highly elongated cells derived from the outer epidermis of ovules. a large number of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the process of fiber development. here we examine the role of the cotton-fiber-specific r2r3 myb gene ghmyb109 in cotton fiber development. transgenic reporter gene analysis revealed that a 2-kb ghmyb109 promoter was sufficient to co ... | 2008 | 18780729 |
| fitness costs associated with cry1ac-resistant helicoverpa zea (lepidoptera: noctuidae): a factor countering selection for resistance to bt cotton? | the heritability, stability, and fitness costs in a cry1ac-resistant helicoverpa zea (boddie) (lepidoptera: noctuidae) colony (ar) were measured in the laboratory. in response to selection, heritability values for ar increased in generations 4-7 and decreased in generations 11-19. ar had significantly increased pupal mortality, a male-biased sex ratio, and lower mating success compared with the unselected parental strain (sc). ar males had significantly more mating costs compared with females. a ... | 2008 | 18767756 |
| survivorship of helicoverpa zea and heliothis virescens on cotton plant structures expressing a bacillus thuringiensis vegetative insecticidal protein. | a series of tests quantified bollworm, helicoverpa zea (boddie), and tobacco budworm, heliothis virescens (f.), larval survival on plant structures of a nontransgenic cotton (gossypium hirsutum l.), 'coker 312', and two transgenic cottons expressing vip3a protein or both vip3a + crylab proteins (vipcot). vegetative and reproductive structures including terminal leaves, flower bud (square) bracts, whole debracted squares, flower petals, flower anthers, and intact capsules (bolls) were harvested f ... | 2008 | 18767734 |
| specific expression of a beta-tubulin gene (ghtub1) in developing cotton fibers. | a cdna library was constructed using poly (a)(+) rna isolated from -1-15 dpa fibers of upland cotton (gossypium hirsutum). the cdna encoding a beta-tubulin isoform (designated as ghtub1) was identified through est search. northern blot analysis using 3'-utr of the cdna as a gene-specific probe was performed to investigate the expression levels of ghtub1 in various organs and in the developing fibers. the results showed that ghtub1 gene was specifically expressed in cotton fiber cells. during fib ... | 2003 | 18763138 |
| down-regulation of ghadf1 gene expression affects cotton fibre properties. | cotton fibre is the most important natural fibres for textile industry. to date, the mechanism that governs the development of fibre traits is largely unknown. in this study, we have characterized the function of a member of the actin depolymerizing factor (adf) family in gossypium hirsutum by down-regulation of the gene (designated as ghadf1) expression in the transgenic cotton plants. we observed that both the fibre length and strength of the ghadf1-underexpressing plants increased as compared ... | 2009 | 18761653 |
| mapping and quantitative trait loci analysis of verticillium wilt resistance genes in cotton. | verticillium wilt is one of the most serious constraints to cotton production in almost all of the cotton-growing countries. in this study, "xinluzao1" (xlz1), a susceptible cultivar gossypium hirsutum l. and "hai7124" (h7124), a resistant line g. barbadense, and their f(2:3) families were used to map and study the disease index induced by verticillium wilt. a total of 430 ssr loci were mapped into 41 linkage groups; the map spanned 3,745.9 cm and the average distance between adjacent loci was 8 ... | 2008 | 18713439 |
| promoter of a cotton fibre myb gene functional in trichomes of arabidopsis and glandular trichomes of tobacco. | cotton fibres are unicellular seed trichomes. our previous study suggested that the cotton r2r3 myb transcript factor gamyb2 is a functional homologue of the arabidopsis trichome regulator glabra1 (gl1). here, the gamyb2 promoter activity is reported in cotton (gossypium hirsutum), tobacco (nicotiana tabacum), and arabidopsis plants. a 2062 bp promoter of gamyb2 was isolated from g. arboreum, and fused to a beta-glucuronidase (gus) reporter gene. in cotton, the gamyb2 promoter exhibited activiti ... | 2008 | 18711121 |
| plant-herbivore-carnivore interactions in cotton, gossypium hirsutum: linking belowground and aboveground. | most studies on plant-herbivore interactions focus on either root or shoot herbivory in isolation, but above- and belowground herbivores may interact on a shared host plant. cotton (gossypium spp.) produces gossypol and a variety of other gossypol-like terpenoids that exhibit toxicity to a wide range of herbivores and pathogens. cotton plants also can emit herbivore-induced volatile compounds at the site of damage and systemically on all tissues above the site of damage. as these volatile compou ... | 2008 | 18709506 |
| cloning and characterization of a tau glutathione s-transferase subunit encoding gene in gossypium hirsutum. | a predicted tau glutathione s-transferase (gst) subunit encoding gene, named ghgst, was isolated from gossypium hirsutum with race method from ssh library based on verticillium dahliae stress. the data revealed an open reading frame of 678 bp encoding a protein of 225 amino acids with a molecular weight of 25.821 kda. semi-quantitative rt-pcr analysis showed that the mrna of ghgst was expressed in root, stem and leaf. and the content of ghgst expression increased under verticillium dahliae stres ... | 2008 | 18670133 |
| silicon carbide whisker-mediated embryogenic callus transformation of cotton (gossypium hirsutum l.) and regeneration of salt tolerant plants. | a silicon carbide whisker-mediated gene transfer system with recovery of fertile and stable transformants was developed for cotton (gossypium hirsutum l.) cv. coker-312. two-month-old hypocotyl-derived embryogenic/non-embryogenic calli at different days after subculture were treated with silicon carbide whiskers for 2 min in order to deliver pgreen0029 encoding gus gene and prg229 avp1 gene, encoding arabidopsis vacuolar pyrophosphatase, having neomycin phosphotransferaseii (nptii) genes as plan ... | 2008 | 18663608 |
| purification and characterization of cell suspensions peroxidase from cotton (gossypium hirsutum l.). | two peroxidases, cpod-i and rpod-ii, have been isolated and purified from cotton cell suspension and their biochemical characteristics studied. rpod-ii from r405-2000, a non-embryogenic cultivar, has higher activity than cpod-i derived from coker 312, which developed an embryogenic structure. the cpod-i and rpod-ii had molecular mass of 39.1 and 64 kda respectively, as determined by sds-page. both enzymes showed high efficiency of interaction with the guaiacol at 25 mm. the optimal ph for cpod-i ... | 2009 | 18649146 |
| geminivirus-mediated gene silencing from cotton leaf crumple virus is enhanced by low temperature in cotton. | a silencing vector for cotton (gossypium hirsutum) was developed from the geminivirus cotton leaf crumple virus (clcrv). the clcrv coat protein gene was replaced by up to 500 bp of dna homologous to one of two endogenous genes, the magnesium chelatase subunit i gene (chli) or the phytoene desaturase gene (pds). cotyledons of cotton cultivar 'deltapine 5415' bombarded with the modified viral vectors manifested chlorosis due to silencing of either chli or pds in approximately 70% of inoculated pla ... | 2008 | 18621976 |
| gene-rich islands for fiber development in the cotton genome. | cotton fiber is an economically important seed trichome and the world's leading natural fiber used in the manufacture of textiles. as a step toward elucidating the genomic organization and distribution of gene networks responsible for cotton fiber development, we investigated the distribution of fiber genes in the cotton genome. results revealed the presence of gene-rich islands for fiber genes with a biased distribution in the tetraploid cotton (gossypium hirsutum l.) genome that was also linke ... | 2008 | 18619771 |
| resistance allele frequency to bt cotton in field populations of helicoverpa armigera (lepidoptera: noctuidae) in china. | resistance evolution in target insects to bacillus thurningiensis (bt) cotton, gossypium hirsutum l., is a main threat to bt cotton technology. an increasing trend of population density of helicoverpa armigera (hübner) (lepidoptera: noctuidae) has been observed since 2001 in qiuxian county (hebei, china), where bt cotton has been planted dominantly since 1998. this region was selected in 2006 and 2007 for estimating frequency of gene alleles conferring resistance to bt cotton by screening the f1 ... | 2008 | 18613597 |
| qtl mapping of yield and fiber traits based on a four-way cross population in gossypium hirsutum l. | four-way cross (4wc) involving four different inbred lines frequently appears in the cotton breeding programs. however, linkage analysis and quantitative trait loci (qtl) mapping with molecular markers in cotton has largely been applied to populations derived from a cross between two inbred lines, and few results of qtl dissection were conducted in a 4wc population. in this study, an attempt was made to construct a linkage map and identify qtl for yield and fiber quality traits in 4wc derived fr ... | 2008 | 18604518 |
| identification of micro-rnas in cotton. | the plant genome has conserved small non-coding micrornas (mirnas) genes about 20-24 nucleotides long. they play a vital role in the gene regulation at various stages of plant life. their conserved nature among the various organisms not only suggests their early evolution in eukaryotes but also makes them a good source of new mirna discovery by homology search using bioinformatics tools. a systematic search approach was used for interspecies orthologues of mirna precursors, from known sequences ... | 2016 | 18603441 |
| molecular cloning, expression profiling, and yeast complementation of 19 beta-tubulin cdnas from developing cotton ovules. | microtubules are a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis. in the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 beta-tubulin genes (tub) cloned from a cotton cdna library. among the group, 12 cotton tubs (ghtubs) are reported for the first time here. transcription profiling revealed that nine ghtubs were highly expressed in elongating fibre cells as compared with fuzzless-li ... | 2008 | 18596112 |
| production of mrna from the cry1ac transgene differs among bollgard lines which correlates to the level of subsequent protein. | commercial cultivars of bollgard cotton, gossypium hirsutum l., differ in the amount of expressed cry1ac protein. however, the plant-mechanism for which this occurs is still unknown. using quantitative real-time polymerase chain reaction (qpcr), we developed a method to determine if differences in the overall level of cry1ac among bollgard lines could be correlated to the mrna transcripts. our data shows that the cry1ac mrna transcript differs among bollgard lines and are correlated with corresp ... | 2009 | 18594999 |
| a preliminary analysis of genome structure and composition in gossypium hirsutum. | upland cotton has the highest yield, and accounts for > 95% of world cotton production. decoding upland cotton genomes will undoubtedly provide the ultimate reference and resource for structural, functional, and evolutionary studies of the species. here, we employed genetrek and bac tagging information approaches to predict the general composition and structure of the allotetraploid cotton genome. | 2008 | 18590573 |
| atg-anchored aflp (atg-aflp) analysis in cotton. | amplified fragment length polymorphism (aflp) marker system has had broad applications in biology. however, the anonymous aflp markers are mainly amplified from non-coding regions, limiting their usefulness as a functional marker system. to take advantages of the traditional aflp techniques, we propose substitution of a restriction enzyme that recognizes a restriction site containing atg, called atg-anchored aflp (atg-aflp) analysis. in this study, we chose nsii (recognizing atgcat) to replace e ... | 2008 | 18584177 |
| co-expression and preferential interaction between two calcineurin b-like proteins and a cbl-interacting protein kinase from cotton. | the cbl/cipk signaling system mediates a variety of responses to environmental stimuli in plants. in this work, we identified four cbl genes from gossypium hirsutum, two of which (designated ghcbl2 and ghcbl3) showed preferential expression in the elongating fiber cells. moreover, the expression patterns of these two cbl genes coincided with that of a putative cbl-interacting protein kinase gene (ghcipk1) that we isolated in a previous study. yeast two-hybrid assay indicated that among the four ... | 2008 | 18573665 |
| chilling stress response of postemergent cotton seedlings. | early season development of cotton is often impaired by sudden episodes of chilling temperature. we determined the chilling response specific to postemergent 13-day-old cotton (gossypium hirsutum l. cv. coker 100a-glandless) seedlings. seedlings were gradually chilled during the dark period and rewarmed during the night-to-day transition. for some chilled plants, the soil temperature was maintained at control level. plant growth, water relations and net photosynthesis (p(n)) were analyzed after ... | 2008 | 18573190 |
| parallel domestication, convergent evolution and duplicated gene recruitment in allopolyploid cotton. | a putative advantage of allopolyploidy is the possibility of differential selection of duplicated (homeologous) genes originating from two different progenitor genomes. in this note we explore this hypothesis using a high throughput, snp-specific microarray technology applied to seed trichomes (cotton) harvested from three developmental time points in wild and modern accessions of two independently domesticated cotton species, gossypium hirsutum and g. barbadense. we show that homeolog expressio ... | 2008 | 18562666 |
| molecular cloning and characterization of a cytosolic glutamine synthetase gene, a fiber strength-associated gene in cotton. | an expressed sequence tag encoding glutamine synthetase (gs) was identified by microarray-based hybridization using fiber mrnas of allotetraploid gossypium hirsutum, 7235, a super quality property germplasm line, and tm-1, a genetic standard in g. hirsutum. northern-blot analysis verified transcript accumulation differences in 8 dpa fibers (including ovules) in the two varieties. the full-length cdna encoding gs in 7235 was isolated and named ghgs. sequence analysis revealed that the ghgs was si ... | 2008 | 18560885 |
| overexpression of an h+-ppase gene from thellungiella halophila in cotton enhances salt tolerance and improves growth and photosynthetic performance. | salinity is one of the major environmental factors limiting plant growth and productivity. an h(+)-ppase gene, tsvp from thellungiella halophila, was transferred into cotton (gossypium hirsutum) in sense and antisense orientations under control of the cauliflower mosaic virus (camv) 35s promoter. southern and northern blotting analysis showed that the sense or antisense tsvp were integrated into the cotton genome and expressed. transgenic plants overexpressing the vacuolar h(+)-ppase were much m ... | 2008 | 18550626 |
| outcrossed cottonseed and adventitious bt plants in arizona refuges. | outcrossing of non-bt cotton (gossypium hirsutum (l.)) in refuges by transgenic bt cultivars could reduce the efficacy of refuges for delaying resistance in seed-feeding pests. based on reports that outcrossing decreased as distance from bt cotton increased in small-scale studies, we hypothesized that increasing refuge width or distance from bt fields would reduce outcrossing. in a large-scale study in arizona, we quantified bt seed in refuges of experimental and commercial fields, comparing out ... | 2008 | 18549770 |
| characteristics and analysis of simple sequence repeats in the cotton genome based on a linkage map constructed from a bc1 population between gossypium hirsutum and g. barbadense. | in the past decade, several molecular maps of cotton have been constructed using diverse dna molecular markers and mapping populations. in this study, an interspecific linkage map of allotetraploid cotton was developed using a bc1 population ((gossypium hirsutum x g. barbadense) x g. hirsutum). this map was genome-wide and was based entirely on simple sequence repeat (ssr) markers. forty-four linkage groups were assigned to 26 chromosomes, with 917 loci spanning 5452.2 cm of the genome. the aver ... | 2008 | 18545277 |
| characterization of 19 novel cotton fla genes and their expression profiling in fiber development and in response to phytohormones and salt stress. | fasciclin-like arabinogalactan proteins (flas), a subclass of arabinogalactan proteins (agps), are usually involved in cell development in plants. to investigate the expression profiling as well as the role of fla genes in fiber development, 19 ghfla genes (cdnas) were isolated from cotton (gossypium hirsutum). among them, 15 are predicted to be glycosylphosphatidylinositol anchored to the plasma membranes. the isolated cotton flas could be divided into four groups. real-time quantitative revers ... | 2008 | 18507812 |
| detection of somaclonal variation of cotton (gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers. | two protocols of plant regeneration for cotton were adopted in this study, namely, 2, 4-d and kinetin hormone combination and iba and kinetin hormone combination. twenty-eight embryogenic cell lines via somatic embryogenesis and 67 regenerated plants from these embryogenic calli were selected and used for random amplified polymorphic dna (rapd), simple sequence repeat (ssr), chromosomal number counting, and flow cytometric analysis. the roles of rapd and ssr markers in detecting somaclonal varia ... | 2008 | 18504587 |
| pb(ii) biosorption from hazardous aqueous streams using gossypium hirsutum (cotton) waste biomass. | studies on the biosorptive ability of gossypium hirsutum (cotton) waste biomass outlined that smaller size of biosorbent (0.355mm), higher biomass dose (0.20g), 5 ph and 100mg/l initial pb(ii) concentration were more suitable for enhanced pb(ii) biosorption from aqueous medium. the langmuir isotherm model and pseudo second order kinetic model fitted well to the data of pb(ii) biosorption. highly negative magnitude of gibbs free energy (deltag degrees ) indicated that the process was spontaneous ... | 2009 | 18502037 |
| urease from cotton (gossypium hirsutum) seeds: isolation, physicochemical characterization, and antifungal properties of the protein. | ureases (ec 3.5.1.5) are metalloenzymes that hydrolyze urea to produce ammonia and carbon dioxide these enzymes, which are found in fungi, bacteria, and plants, show very similar structures. despite an abundance of urease in vegetal tissues, the physiological role of this enzyme in plants is still poorly understood. it has been previously described that ureases from the legumes jackbean ( canavalia ensiformis) and soybean ( glycine max) have insecticidal activity and antifungal properties. this ... | 2008 | 18494485 |
| genetic fingerprinting of australian cotton cultivars with rapd markers. | rapd (random amplified polymorphic dna) markers generated by 30 random decamer primers were used to fingerprint 12 released cultivars and a breeding line of gossypium hirsutum and 1 cultivar of g. barbadense presently under cultivation in australia. among a total of 453 developed markers, 69 (15.2%) were only present (unique) in the g. barbadense cultivar pima s-7. of the remaining markers, 128 (33.3%) were fixed in all 13 g. hirsutum cultivars. in pairwise comparisons of the degree of band shar ... | 1995 | 18470223 |