Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [sequence and structure analysis of cryptic plasmid pn30 from oil-oxidizing strain rhodococcus erythropolis 30]. | nucleotide sequence of cryptic plasmid pn30 from a rhodococcus erythropolis 30 soil isolate was determined. plasmid dna consists of 5403 nucleotide pairs and contains about 62% gc pairs, which is typical of rhodococcus dna. no significant homology was determined between the pn30 dna sequence and those of known plasmids. computer-aided analysis of pn30 sequence revealed open reading frames that encode proteins strongly homologous to replicative proteins encoded by small cryptic plasmids of differ ... | 2005 | 16396462 |
| identification of a carboxylesterase-producing rhodococcus soil isolate. | subtropical soil microbial isolates were screened for carbohydrate, tributyrin, or olive oil hydrolysis using agar plates supplemented with the corresponding substrates. a heterotrophic, aerobic, gram-positive strain displaying activity on tributyrin was selected and further characterized. analysis of the morphological and physiological traits of the strain placed it as a member of the genus rhodococcus. further 16s rdna sequencing revealed a 99% identity to rhodococcus erythropolis. the strain ... | 2005 | 16391653 |
| sequence analysis of two cryptic plasmids from bifidobacterium longum djo10a and construction of a shuttle cloning vector. | bifidobacterium longum djo10a is a recent human isolate with probiotic characteristics and contains two plasmids, designated pdojh10l and pdojh10s. the complete sequences of both these plasmids have now been determined and consist of two circular dna molecules of 10,073 and 3,661 bp, with g+c contents of 62.2% and 66.2%, respectively. plasmid pdojh10l is a cointegrate plasmid consisting of dna regions exhibiting very high sequence identity to two other b. longum plasmids, pnac2 (98%) and pkj50 ( ... | 2006 | 16391088 |
| aerobic biodegradation of 2,2'-dithiodibenzoic acid produced from dibenzothiophene metabolites. | dibenzothiophene is a sulfur heterocycle found in crude oils and coal. the biodegradation of dibenzothiophene through the kodama pathway by pseudomonas sp. strain bt1d leads to the formation of three disulfides: 2-oxo-2-(2-thiophenyl)ethanoic acid disulfide, 2-oxo-2-(2-thiophenyl)ethanoic acid-2-benzoic acid disulfide, and 2,2'-dithiodibenzoic acid. when provided as the carbon and sulfur source in liquid medium, 2,2'-dithiodibenzoic acid was degraded by soil enrichment cultures. two bacterial is ... | 2006 | 16391083 |
| deep desulfurization of diesel oil and crude oils by a newly isolated rhodococcus erythropolis strain. | the soil-isolated strain xp was identified as rhodococcus erythropolis. r. erythropolis xp could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that exists in crude oil. the desulfurization product of benzonaphthothiophene was identified as alpha-hydroxy-beta-phenyl-naphthalene. resting cells could desulfurize diesel oil (total organic sulfur, 259 ppm) after hydrodesulfurization. the sulfur content of diesel oil was reduced by 94.5% by using the resting cell b ... | 2006 | 16391024 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| rhodococcus ruber as a cause of keratitis. | to report a case of suppurative keratitis caused by rhodococcus ruber in a healthy 31-year-old male patient. | 2006 | 16371792 |
| gene expression analysis using a modified hicep method applicable to prokaryotes: a study of the response of rhodococcus to isoniazid and ethambutol. | we established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the hicep (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. following the removal of abundant rrna, the mrna of prokaryotic cells was enzymatically polyadenylated and subjected to hicep analysis. this method was highly reproducible due to selective pcr that was performed by using adaptor specific primers. we confirmed indu ... | 2006 | 16360900 |
| characterization of the putative operon containing arylamine n-acetyltransferase (nat) in mycobacterium bovis bcg. | mycobacterium bovis bcg and mycobacterium tuberculosis possess a single arylamine n-acetyltransferase whose gene is predicted to occur within a six-gene operon. deletion of the nat gene caused an extended lag phase in m. bovis bcg and a cell morphology associated with an altered pattern of cell wall mycolates. analysis of cdna from m. bovis bcg shows that during in vitro growth all the genes in the putative nat operon are expressed and the open reading frames are contiguous, supporting the exist ... | 2006 | 16359327 |
| [bioconversion of beta-sitosterol and its complex esters by rhodococcus actinobacteria]. | the ability of pure cultures of rhodococcus actinobacteria from the ural specialized collection of alkanotrophic microorganisms (world federation for culture collections accession number 768; http://www.ecology.psu.ru/iegmcol) to convert beta-sitosterol (bss) and its 3beta-acylated derivatives was studied. rhodococcus strains with pronounced cholesterol oxidase activity, capable of converting bss to stigmat-4-ene-3-one in the reaction of cooxidation with n-hexadecane, were selected. the dependen ... | 2005 | 16358751 |
| initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene. | because of its high electron deficiency, initial microbial transformations of 2,4,6-trinitrotoluene (tnt) are characterized by reductive rather than oxidation reactions. the reduction of the nitro groups seems to be the dominating mechanism, whereas hydrogenation of the aromatic ring, as described for picric acid, appears to be of minor importance. thus, two bacterial strains enriched with tnt as a sole source of nitrogen under aerobic conditions, a gram-negative strain called tnt-8 and a gram-p ... | 1998 | 16349484 |
| efficient transformation of the cephamycin c producer nocardia lactamdurans and development of shuttle and promoter-probe cloning vectors. | a high transformation efficiency (1 x 10 to 7 x 10 transformants per mug of dna) of nocardia lactamdurans lc411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. a variety of vectors from streptomyces lividans, brevibacterium lactofermentum, rhodococcus fascians, and a nocardia (amycolatopsis) sp. were tested; transformants could be obtained only with vectors derived from an endogenous plasmid of the amycolatopsis sp. strain dsm 43387. vectors carryi ... | 1994 | 16349436 |
| adsorption of rhodococcus strain gin-1 (ncimb 40340) on titanium dioxide and coal fly ash particles. | rhodococcus strain gin-1 (ncimb 40340) can be used to enrich and isolate a titanium-rich fraction from coal fly ash. the gram-positive bacterium was isolated by its ability to adhere strongly and rapidly to suspended particles of pure titanium dioxide or coal fly ash. adsorption depends on the salt concentration and occurs in seawater. lowering of the salt concentration or washing of particles with pure water did not, however, cause desorption of the bacteria from tio(2) particles; this was achi ... | 1994 | 16349369 |
| cesium accumulation and growth characteristics of rhodococcus erythropolis cs98 and rhodococcus sp. strain cs402. | growth and cesium accumulation characteristics of two cesium-accumulating bacteria isolated from soils were investigated. rhodococcus erythropolis cs98 and rhodococcus sp. strain cs402 accumulated high levels of cesium (approximately 690 and 380 mumol/g [dry weight] of cells or 92 and 52 mg/g [dry weight] of cells, respectively) after 24 h of incubation in the presence of 0.5 mm cesium. the optimum ph for cesium uptake by both rhodococcus strains was 8.5. rubidium and cesium assumed part of the ... | 1994 | 16349312 |
| purification and characterization of an inducible s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r. | the widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. few microbial isolates capable of transforming substituted s-triazines have been identified. rhodococcus corallinus nrrl b-15444 has previously been shown to possess a hydrolase activity that is responsible for the dechlorination of the triazine compounds deethylsimazine (6-chloro-n-ethyl-1,3,5-triazine-2,4-diamine) ... | 1994 | 16349190 |
| aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by rhodococcus species. | two rhodococcus strains which were isolated from a trichloroethylene (tce)-degrading bacterial mixture and rhodococcus rhodochrous atcc 21197 mineralized vinyl chloride (vc) and tce. greater than 99.9% of a 1-mg/liter concentration of vc was degraded by cell suspensions. [1,2-c]vc was degraded by cell suspensions, with the production of greater than 66% co(2) and 20% c-aqueous phase products and incorporation of 10% of the c into the biomass. cultures that utilized propane as a substrate were ab ... | 1994 | 16349184 |
| selective desulfurization of dibenzothiophene by rhodococcus erythropolis d-1. | a dibenzothiophene (dbt)-degrading bacterium, rhodococcus erythropolis d-1, which utilized dbt as a sole source of sulfur, was isolated from soil. dbt was metabolized to 2-hydroxybiphenyl (2-hbp) by the strain, and 2-hbp was almost stoichiometrically accumulated as the dead-end metabolite of dbt degradation. dbt degradation by this strain was shown to proceed as dbt --> dbt sulfone --> 2-hbp. dbt at an initial concentration of 0.125 mm was completely degraded within 2 days of cultivation. dbt at ... | 1994 | 16349153 |
| physicochemical cell surface and adhesive properties of coryneform bacteria related to the presence and chain length of mycolic acids. | the presence and chain length of mycolic acids of bacteria of the genera corynebacterium, rhodococcus, gordona, mycobacterium, and arthrobacter and of coryneform bacteria containing a type b peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cells to defined surfaces such as teflon and glass. the origin of the overall negative charge of these bacteria is discussed. | 1993 | 16349100 |
| identification and cloning of genes involved in specific desulfurization of dibenzothiophene by rhodococcus sp. strain igts8. | the gram-positive bacterium rhodococcus sp. strain igts8 is able to remove sulfur from certain aromatic compounds without breaking carbon-carbon bonds. in particular, sulfur is removed from dibenzothiophene (dbt) to give the final product, 2-hydroxybiphenyl. a genomic library of igts8 was constructed in the cosmid vector plafr5, but no desulfurization phenotype was imparted to escherichia coli. therefore, igts8 was mutagenized, and a new strain (uv1) was selected that had lost the ability to des ... | 1993 | 16349035 |
| novel allylic oxidation of alpha-cedrene to sec-cedrenol by a rhodococcus strain. | a bacterial strain, designated ksm-7358, that can use alpha-cedrene for growth was isolated. the strain was identified as a member of the genus rhodococcus and catalyzed the novel allylic oxidation of alpha-cedrene regiospecifically to produce (r)-10-hydroxycedrene (sec-cedrenol) with a very high yield. alpha-curcumene was also produced as a possible metabolite of sec-cedrenol. a possible pathway for the microbial conversion of alpha-cedrene to sec-cedrenol and alpha-curcumene is proposed. | 1993 | 16348930 |
| stereospecificity of microbial hydrations of oleic acid to 10-hydroxystearic acid. | we recently described a simple method for ascertaining the stereochemical purities of hydroxy fatty acids (s. h. el-sharkawy, w. yang, l. dostal, and j. p. n. rosazza, appl. environ. microbiol. 58:2116-2122, 1992) based on the h-nuclear magnetic resonance spectral analysis of diastereomeric s-(+)-o-acetylmandelate esters of hydroxystearates. this report describes the stereochemistries of microbial hydrations of oleic acid to 10-hydroxystearic acid by nocardia aurantia (also known as rhodococcus ... | 1993 | 16348853 |
| transformation of rhodococcus fascians by high-voltage electroporation and development of r. fascians cloning vectors. | the analysis of the virulence determinants of phytopathogenic rhodococcus fascians has been hampered by the lack of a system for introducing exogenous dna. we investigated the possibility of genetic transformation of r. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid dna. electrotransformation in r. fascians d188 resulted in transformation frequencies ranging from 10/mug of dna to 10/mug of dna, depending on the dna concentration. the effects of diff ... | 1990 | 16348290 |
| coexisting bacterial populations responsible for multiphasic mineralization kinetics in soil. | experiments were conducted to study populations of indigenous microorganisms capable of mineralizing 2,4-dinitrophenol (dnp) in two soils. previous kinetic analyses indicated the presence of two coexisting populations of dnp-mineralizing microorganisms in a forest soil (soil 1). studies in which eucaryotic and procaryotic inhibitors were added to this soil indicated that both populations were bacterial. most-probable-number counts with media containing different concentrations of dnp indicated t ... | 1990 | 16348277 |
| improved method for the isolation of biosurfactant glycolipids from rhodococcus sp. strain h13a. | an improved method for the isolation of the biosurfactant glycolipids from rhodococcus sp. strain h13a by using xm 50 diafiltration and isopropanol precipitation was devised. this procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. the protein apparently does not contribute any biosurfactant characteristics to the glycolipids. the deacylated glycolipid backbone included only a disaccharide. | 1990 | 16348198 |
| initial steps in the degradation of phosphinothricin (glufosinate) by soil bacteria. | three hundred bacterial isolates from soil were tested for resistance against phosphinothricin [ppt; dl-homoalanin-4-yl(methyl)phosphinic acid], the active ingredient of the herbicide basta. eight resistant bacterial strains and escherichia coli were analyzed for ppt-transforming activities. at least three different enzymatic reactions could be detected in cell extracts. in six strains an acetyltransferase was active, synthesizing n-acetyl-ppt in the presence of ppt and acetyl coenzyme a. all st ... | 1989 | 16347878 |
| hydroxylation and dechlorination of tetrachlorohydroquinone by rhodococcus sp. strain cp-2 cell extracts. | a cell extract of a polychlorophenol-degrading bacterium, rhodococcus sp. strain cp-2, isolated from chlorophenol-contaminated soil, was shown to dechlorinate tetrachlorohydroquinone, the first intermediate in pentachlorophenol and 2,3,5,6-tetrachlorophenol degradation. degradation of tetrachlorohydroquinone was catalyzed by a soluble enzyme(s). the reaction sequence for complete dechlorination involved hydroxylation and three reductive dechlorinations, producing 1,2,4-trihydroxybenzene. all chl ... | 1989 | 16347859 |
| o-methylation of chlorinated para-hydroquinones by rhodococcus chlorophenolicus. | rhodococcus chlorophenolicus pcp-i, a degrader of polychlorinated phenols, guaiacols (2-methoxyphenols), and syringols (2,6-dimethoxyphenols), was shown to o-methylate the degradation intermediate, a chlorinated para-hydroquinone, into 4-methoxyphenol. o-methylation was constitutively expressed, whereas the degradation of chlorophenols and chlorohydroquinones was inducible in r. chlorophenolicus. the o-methylating reaction required two hydroxyl groups in positions para to each other. r. chloroph ... | 1988 | 16347691 |
| nitrile hydratase-catalyzed production of nicotinamide from 3-cyanopyridine in rhodococcus rhodochrous j1. | nitrile hydratase, which occurs abundantly in cells of rhodococcus rhodochrous j1 isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. by using resting cells, the reaction conditions for nicotinamide production were optimized. under the optimum conditions, 100% of the added 12 m 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixture containin ... | 1988 | 16347686 |
| nitrilase-catalyzed production of nicotinic acid from 3-cyanopyridine in rhodococcus rhodochrous j1. | the nitrilase which occurs abundantly in cells of rhodococcus rhodochrous j1 catalyzes the direct hydrolysis of 3-cyanopyridine to nicotinic acid without forming nicotinamide. by using resting cells, the reaction conditions for nicotinic acid production were optimized. under the optimum conditions, 100% of the added 3-cyanopyridine could be converted to nicotinic acid, the highest yield achieved being 172 mg of nicotinic acid per 1.0 ml of reaction mixture containing 2.89 mg (dry weight) of cell ... | 1988 | 16347598 |
| methylation of halogenated phenols and thiophenols by cell extracts of gram-positive and gram-negative bacteria. | o-methylation of 2,6-dibromophenol was studied in cell extracts prepared from rhodococcus sp. strain 1395. o-methylation activity was enhanced by the addition of s-adenosyl-l-methionine but was not affected by the addition of 5-methyltetrahydrofolate nor by up to 10 mm mgcl(2) or edta. by using 2,6-dibromophenol, 4,5,6-trichloroguaiacol, and pentachlorothiophenol as the substrates, o-methylation activity was also demonstrated in extracts from two other rhodococcus sp. strains, an acinetobacter s ... | 1988 | 16347565 |
| one-step microbial conversion of a racemic mixture of pantoyl lactone to optically active d-(-)-pantoyl lactone. | washed cells of rhodococcus erythropolis ifo 12540 were found to convert only the l-(+)-isomer of pantoyl lactone to the d-(-)-isomer in a racemic mixture of pantoyl lactone. under suitable reaction conditions, the amount of d-(-)-pantoyl lactone synthesized was 18.2 mg/ml (94.4% enantiomer excess; molar yield, 90.5%). this conversion was suggested to proceed through the following successive reactions: first, the enzymatic oxidation of l-(+)-pantoyl lactone to ketopantoyl lactone; second, the ra ... | 1987 | 16347301 |
| role of carbon dioxide in catabolism of propane by "nocardia paraffinicum" (rhodococcus rhodochrous). | the catabolism of propane by "nocardia paraffinicum" (rhodococcus rhodochrous) has been shown to involve co(2) fixation after its oxidation to propionic acid. "n. paraffinicum" failed to grow on either propane or 1-propanol in the absence of co(2). the rate of propane utilization was directly related to the initial co(2) concentration, and warburg respirometry suggested that co(2) was required for the catabolism of 1-propanol, propionaldehyde, and propionate but not for 2-propanol. these data al ... | 1987 | 16347267 |
| new type of oxygenase involved in the metabolism of propane and isobutane. | nocardia paraffinicum (rhodococcus rhodochrous), a hydrocarbon-degrading microorganism, was used in a study of propane and isobutane metabolism. the bacterium was able to utilize propane or isobutane as a sole source of carbon, and oxygen was found to be essential for its metabolism. gas chromatographic analysis showed that n-propanol was the major compound recovered from the metabolism of propane by resting cells, although trace amounts of isopropanol and acetone were detected. when a mixture o ... | 1984 | 16346605 |
| chemical and physical characterization of interfacial-active lipids from rhodococcus erythropolis grown on n-alkanes. | lipophilic compounds of the culture suspension containing rhodococcus erythropolis dsm43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. thirteen main components from a dichloromethane-methanol extract of the r. erythropolis cultures were isolated and characterized to specify quantitatively their surfactant properties, e.g., minimum surface and interfacial tensions and critical micelle concentrations. the interfacial activity of the organi ... | 1982 | 16346110 |
| [comparison of the desulfurization activity among several bacteria and analysis of the conservation of their desulfurization genes]. | several bacteria, bacillus brevis r-6, pseudomonas delafleldii r-8, nocardia globerula r-9, bacillus sphaericus r-16, rhodococcus erythropolis lsse8-1 and gordonia nitida lssej-1, which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate, were investigated. desulfurization products were quantitively determined by hplc. result revealed that each of these bacteria desulfurize dbt at a different rate. in order to obtain more information, the fragments encoding desulfurizing enzymes were ... | 2005 | 16342766 |
| aliphatic amidase from rhodococcus rhodochrous m8 is related to the nitrilase/cyanide hydratase family. | a comparative study of amino acid sequence and physicochemical properties indicates the affiliation of an amidase from rhodococcus rhodochrous m8 (ec 3.5.1.4) to the nitrilase/cyanide hydratase family. cluster analysis and multiple alignments show that cys166 is an active site nucleophile. the enzyme has been shown to be a typical aliphatic amidase, being the most active toward short-chain linear amides. small polar molecules such as hydroxylamine and o-methyl hydroxylamine can serve as effectiv ... | 2005 | 16336190 |
| isolation and characterization of o-xylene oxygenase genes from rhodococcus opacus tkn14. | o-xylene is one of the most difficult-to-degrade environmental pollutants. we report here rhodococcus genes mediating oxygenation in the first step of o-xylene degradation. rhodococcus opacus tkn14, isolated from soil contaminated with o-xylene, was able to utilize o-xylene as the sole carbon source and to metabolize it to o-methylbenzoic acid. a cosmid library from the genome of this strain was constructed in escherichia coli. a bioconversion analysis revealed that a cosmid clone incorporating ... | 2005 | 16332743 |
| a soil-based microbial biofilm exposed to 2,4-d: bacterial community development and establishment of conjugative plasmid pjp4. | a soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-d) (25 microg ml(-1)). culturable bacterial members of the community were identified by 16s rrna gene sequencing and found to be members of the genera pseudomonas, burkholderia, collimonas and rhodococcus. a 2,4-d degrading donor strain, pseudomonas putida sm1443 (pjp4::gfp), was inoculated into flow cell chambers containing 2-day old biofil ... | 2005 | 16332330 |
| degradation of hydrocarbons and alcohols at different temperatures and salinities by rhodococcus erythropolis dcl14. | rhodococcus erythropolis dcl14 cells were able to metabolise c5-c16 hydrocarbons and c1-c12 alcohols as sole carbon and energy sources, both at 15 and 28 degrees c. metabolic activity was also observed at 1.00%, 1.95% and 2.50% sodium chloride. almost complete degradation of n-, iso- and cyclo-alkanes and aromatic compounds present in fuel oil was achieved after 9 months, 60% being consumed in the first three months. the results from the conditions tested here suggest that this type of bacterium ... | 2004 | 16329886 |
| immobilization of hydrocarbon-oxidizing bacteria in poly(vinyl alcohol) cryogels hydrophobized using a biosurfactant. | a simple biosurfactant-based hydrophobization procedure for poly(vinyl alcohol) (pva) cryogels was developed allowing effective immobilization of hydrocarbon-oxidizing bacteria. the resulting partially hydrophobized pva cryogel granules (granule volume 5 microl) contained sufficient number (6.5 x 10(3)) of viable bacterial cells per granule, possessed high mechanical strength and spontaneously located at the interface in water-hydrocarbon system. such interfacial location of pva granules allowed ... | 2006 | 16316701 |
| metabolites from the biodegradation of di-ester plasticizers by rhodococcus rhodochrous. | the plasticizers di-2-ethylhexyl phthalate (dehp) and di-2-ethylhexyl adipate (deha) are ubiquitous in the environment and undergo partial biodegradation in the presence of soil micro-organisms. the validity of a proposed pathway for the degradation of these plasticizers by rhodococcus rhodochrous has been confirmed by the identification of 2-ethylhexanal in gas phase emissions. complete analyses of the aqueous and gas phases were able to account for more than 98% of the 2-ethylhexanol component ... | 2006 | 16316677 |
| biochemical pathway and degradation of phthalate ester isomers by bacteria. | degradation of dimethyl isophthalate (dmi) and dimethyl phthalate ester (dmpe) was investigated using microorganisms isolated from mangrove sediment of hong kong mai po nature reserve. one enrichment culture was capable of utilizing dmi as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading dmi alone. in co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (mmi) ester and isophthalic acid (ip ... | 2005 | 16312973 |
| survey of zoonoses recorded in scotland between 1993 and 2002. | all the human and animal laboratory reports of zoonoses sent to health protection scotland between 1993 and 2002 were identified. there were 24,946 reports from veterinary laboratories, and 94,718 (20 per cent) of the 468,214 reports from medical laboratories were considered to be zoonotic. the most common reports of zoonoses from people were campylobacter, salmonella, cryptosporidium and giardia species and escherichia coli o157. the most common reports of zoonoses from animals were salmonella, ... | 2005 | 16311383 |
| bacterial degradation of xenobiotic compounds: evolution and distribution of novel enzyme activities. | bacterial dehalogenases catalyse the cleavage of carbon-halogen bonds, which is a key step in aerobic mineralization pathways of many halogenated compounds that occur as environmental pollutants. there is a broad range of dehalogenases, which can be classified in different protein superfamilies and have fundamentally different catalytic mechanisms. identical dehalogenases have repeatedly been detected in organisms that were isolated at different geographical locations, indicating that only a res ... | 2005 | 16309386 |
| fluorescent phospholipid analogs as microscopic probes for detection of the mycolic acid-containing layer in corynebacterium glutamicum: detecting alterations in the mycolic acid-containing layer following ethambutol treatment. | corynebacterium glutamicum belongs to the mycolic acid-containing actinomycetes, which also include mycobacterium, nocardia, and rhodococcus. the cells of this group possess a cell wall with a thick outer layer composed primarily of mycolic acid, which functions as a permeability barrier. to investigate the mechanism of mycolic acid-containing layer (mycolate layer) formation, we have developed a fluorescence microscopic technique detecting the mycolate layer in situ. the staining specificity of ... | 2005 | 16306684 |
| caprine vertebral osteomyelitis caused by rhodococcus equi. | there have been reports of rhodococcus equi infections in goats in australia, america and india. in this study, r. equi was isolated from an inflamed vertebra in a boer goat in south africa. at autopsy, there was a purulent inflammatory reaction in the 1st cervical vertebra. histopathologically, a neutrophilic infiltration was encountered in the bone. aerobic culture of swabs collected from the abscesses yielded r. equi in pure culture that was identified on biochemical tests. r. equi has become ... | 2005 | 16300185 |
| bacteremia due to rhodococcus equi--a case report. | rhodococcus equi (r. equi) primarily causes zoonotic infections affecting grazing animals and is an unusual cause of infection in immunocompetent human beings. we report a case of bacteremia due to r. equi a rare isolate in a child suffering from protein energy malnutrition | 2004 | 16295396 |
| characterization of four rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols. | four genes were isolated and characterized for alcohol dehydrogenases (adhs) catalyzing the oxidation of aromatic alcohols such as benzyl alcohol to their corresponding aldehydes, one from o-xylene-degrading rhodococcus opacus tkn14 and the other three from n-alkane-degrading rhodococcus erythropolis pr4. various aromatic alcohols were bioconverted to their corresponding carboxylic acids using escherichia coli cells expressing each of the four adh genes together with an aromatic aldehyde dehydro ... | 2006 | 16292529 |
| isolation of pah-degrading bacteria from mangrove sediments and their biodegradation potential. | surface sediment samples were collected from seven mangrove swamps in hong kong sar with different degrees of contamination. the total concentrations of 16 pahs in these sediments ranged from 169.41 to 1058.37 ng g(-1) with the highest concentration found in ma wan and the lowest in kei ling ha lo wai mangrove swamp. in each swamp, three bacterial consortia were enriched from sediments using phenanthrene (phe) as the sole carbon and energy source, and individual bacterial colony showing phe degr ... | 2005 | 16291206 |
| development of a microtiter plate-based method for determination of degradation profile of nitrophenolic compounds. | a microtiter plate-based assay was developed for the automatic monitoring of degradation profile of the yellow-coloured nitrophenolic compounds. the method enables to reduce the intervals between measurements of substrate concentration to minutes and to overcome the problem of discontinuity of sampling typical for conventional methods. the concentrations of nitrophenolic compounds were calculated from the absorbance values determined automatically by bioscreen c. verification of the method was b ... | 2006 | 16289394 |
| [study on the degradation pathway of biphenyl by rhodococcus pyridinovorans r04]. | metabolites of rhodococcus pyridinovorans r04 from biphenyl were analyzed by gc-ms. the results demonstrated that biphenyl was degraded via a route of 2,3-dihydro-2,3-dihydroxybiphenyl and 2,3-dihydroxybiphenyl. 4-benzoylbutyric acid and 1-phenylethanone were also found in the gc-ms analysis, which indicated there were other pathways beside the main route. | 2003 | 16281565 |
| evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes. | pcr was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (acc) deaminase genes from bacteria. the shimodaira-hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (hgt) were significantly favored over vertical transmission. the best maximum likelihood (ml) acc deaminase tree was significantly more likely than four alternative ml trees, suggesting hgt. | 2005 | 16269802 |
| effect of a metal alloy fuel catalyst on bacterial growth. | many microorganisms have been demonstrated to utilize petroleum fuel products to fulfill their nutritional requirement for carbon. as a result, the ability of these microbes to degrade fuel has both a deleterious affect as well as beneficial applications. this study focused on the undesired ability of bacteria to grow on fuel and the potential for some metal alloys to inhibit this biodegradation. the objective of this study was to review the pattern of growth of two reference strains of petroleu ... | 2005 | 16262333 |
| [biodegradation of di-n-butyl phthalate by mangrove microorganism rhodococcus ruber 1k]. | a di-n-butyl phthalate (dbp)degrading bacterium rhodococcus ruber was isolated from mangrove soil, and its degrading characteristics were studied. the results showed that the bacterium could grow well on the substrate with dbp as the sole source of carbon and energy, and the dbp of 50 mg x l(-1) could be completely degraded after 48 h. under aerobic condition, the tentative pathway proposed for dbp degradation was through monoester initially, then phthalic acid, and finally co2 and h2o. | 2005 | 16262080 |
| rhodococcus phenolicus sp. nov., a novel bioprocessor isolated actinomycete with the ability to degrade chlorobenzene, dichlorobenzene and phenol as sole carbon sources. | the aerobic degradation of phenol, chlorobenzene and dichlorobenzene as a sole carbon source has been observed in bacterial gram-positive strain g2pt isolated from a wastewater bioprocessor. cells display branching mycelia fragmenting into rod and coccoid elements when grown on tsa. aerial hyphae formation occurs when grown on phenol and chlorinated aromatics as the sole carbon source. growth was observed at up to 0.75% phenol as a sole carbon source, indicating a strong tolerance for the compou ... | 2005 | 16261859 |
| [the innate ability of rhodococcus sp. sduzawq to tolerate sulfur in petroleum]. | removal of sulfur from petroleum can be accomplished by various means. one method is to use microorganisms, such as bacteria. in the present study, strain rhodococcus sp. sduzawq was employed to test the effects of various concentrations of dibenzothiophene (dbt) and sulfate, had on this removal process. desulfurization was accomplished, using basal salts medium (bsm), supplemented with 0.2mmol/l dbt and different concentrations of na2so4. growth of sduzawq was pronounced, even when the concentr ... | 2005 | 16245874 |
| a proteomic approach to investigate immunity against r. equi in foals. | 2005 | 16244959 | |
| chemical and biochemical transformations of 5-ethoxycarbonyl-5-phenyl-2-isoxazolines. | the salts, 2-methyl-5,5-disubstituted 4,5-dihydroisoxazolium methylsulfates comprising various substituents at the c-3 carbon atom were subjected to transformations. the structure of applied compounds permitted to monitor the effect of this factor on the transformation course of the 2-isoxazoline ring. the nucleophilic addition of cyanide anion to the selected salts enabled the obtaining of a next heterocyclic system of changed physicochemical and biological properties in comparison to the start ... | 2005 | 16243323 |
| a new microbial method for more efficient production of nalpha-benzyloxycarbonyl-l-aminoadipate delta-semialdehyde and nalpha-benzyloxycarbonyl-d-aminoadipate delta-semialdehyde. | a new biochemical method for more efficient production of nalpha-benzyloxycarbonyl-l-aminoadipate delta-semialdehyde (nalpha-z-l-aasa) and nalpha-z-d-aasa was developed with cells of rhodococcus sp. aiu z-35-1. using the cells harvested after 1 d of cultivation, more than 95 mm nalpha-z-l-aasa was produced from 100 mm nalpha-z-l-lysine by incubating at ph 5.0 for 1 d at 30 degrees c or by incubating at ph 7.0 for 2 d at 10 degrees c. a similar conversion yield of nalpha-z-d-aasa was also obtaine ... | 2005 | 16243278 |
| [heterogeneity of rhodococcus opacus 1cp as a response to stress induced by chlorophenols]. | dissociation of rhodococcus opacus 1cp during culturing in different media (containing phenol and its monochlorinated derivatives as the sole source of carbon and energy) was studied. three variants of strain 1cp (s1, s2, and r) differing in the morphology of cells and colonies, lipid composition, and manner of growth on phenol and monochlorophenols were isolated. it was shown that 2- and 4-chlorophenols were most actively degraded by the smooth (s) forms of the culture, and that the rough (r) f ... | 2005 | 16240653 |
| immune response to the rhodococcus equi infection in high and low antibody-producing mice (selection iv-a). | rhodococcus equi is a gram-positive, facultative intracellular bacterium which infects macrophages and causes rhodococcal pneumonia and enteritis in foals. recently, this agent has been recognized as an opportunistic pathogen for immunocompromised humans. several murine experimental models have been used to study r. equi infection. high (h(iv-a)) and low (l(iv-a)) antibody (ab)-producers mice were obtained by bi-directional genetic selections for their ability to produce antibodies against sheep ... | 2005 | 16237269 |
| structure and increased thermostability of rhodococcus sp. naphthalene 1,2-dioxygenase. | rieske nonheme iron oxygenases form a large class of aromatic ring-hydroxylating dioxygenases found in microorganisms. these enzymes enable microorganisms to tolerate and even exclusively utilize aromatic compounds for growth, making them good candidates for use in synthesis of chiral intermediates and bioremediation. studies of the chemical stability and thermostability of these enzymes thus become important. we report here the structure of free and substrate (indole)-bound forms of naphthalene ... | 2005 | 16237006 |
| characterization of floating activity of indigenous diesel-assimilating bacterial isolates. | six diesel-degrading bacterial strains were isolated from oil-polluted sites located in central taiwan. the floating activity of the isolates in an oil-supplemented liquid medium was monitored. cell-surface hydrophobicity as well as cell-free and cell-residue emulsification activities were also investigated. three isolates, identified as gordonia alkanivorans cc-jg 39, rhodococcus erythropolis cc-bc 04, and r. erythropolis cc-bc 11, were found to float and grow near the diesel layer on the surfa ... | 2005 | 16233818 |
| development of a genetic transformation system for benzene-tolerant rhodococcus opacus strains. | rhodococcus opacus b-4 and b-9 are tolerant to various organic solvents including benzene, toluene, ethylbenzene, xylenes and styrene, and are suitable bacterial hosts for the production of chemical products from hydrophobic substrates. a 4.4-kb endogenous plasmid (pknr 01) was isolated from r. opacus b-4 and sequenced completely. plasmid pknr 01 encodes proteins that share similarity to replication proteins from the enteric bacterial and actinomycete theta-replication plasmids. a 7.4-kb chimeri ... | 2005 | 16233810 |
| isolation and characterization of benzene-tolerant rhodococcus opacus strains. | twenty-two benzene-utilizing bacteria were isolated from soil samples. among them, three isolates were highly tolerant to benzene. they grew on benzene when liquid benzene was added to the basal salt medium at 10--90% (v/v). taxonomical analysis identified the benzene-tolerant isolates as rhodococcus opacus. one of the benzene-tolerant isolates, designated b-4, could utilize many aromatic and aliphatic hydrocarbons including benzene, toluene, styrene, xylene, ethylbenzene, propylbenzene, n-octan ... | 2005 | 16233805 |
| effect of sulfur sources on specific desulfurization activity of rhodococcus erythropolis ka2-5-1 in exponential fed-batch culture. | the effects of sulfur sources on the desulfurization activity of rhodococcus erythropolis ka2-5-1 were investigated by using an exponential fed-batch culture technique. the feed rate of a sulfur source was controlled independently of the feed rate of ethanol, which was used as a carbon and energy source. among the sulfur sources examined were dibenzothiophene (dbt), ammonium sulfate, l-cysteine, l-methionine, and 2-amino-ethanesulfonic acid. when the fed-medium contained dbt as the sole sulfur s ... | 2005 | 16233786 |
| constitutive synthesis, purification, and characterization of catechol 1,2-dioxygenase from the aniline-assimilating bacterium rhodococcus sp. an-22. | a catechol 1,2-dioxygenase (cd) was found, which was synthesized constitutively in the aniline-assimilating bacterium rhodococcus sp. an-22 grown on a medium without aniline, as well as on aniline medium. the bacterium synthesized cd in its cells grown on all the 21 non-aromatic substrates examined, including four natural media such as meat and yeast extracts, one sugar, six organic acids, and 10 amino acids as carbon, energy, and nitrogen sources. when the bacterium was incubated on a medium wi ... | 2004 | 16233669 |
| characteristics of rhodococcus pyridinovorans pyj-1 for the biodegradation of benzene, toluene, m-xylene (btx), and their mixtures. | the degradation rates of benzene, toluene, and m-xylene (btx) by rhodococcus pyridinovorans pyj-1, were highest at 30, 50, and 25 mg/l, respectively. the degradation rate was highest for toluene (0.106 mg/mg-protein x h) followed by benzene and m-xylene at the optimum ph and temperature of 7 and 32 degrees c, respectively. for btx mixtures, toluene was the preferred substrate, but degradation of each btx was competitively inhibited by other btx compounds. the degradation rate of each component o ... | 2004 | 16233656 |
| aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer rhodococcus sp. n-771. | we identified an aldoxime dehydratase (oxd) gene in the 5'-flanking region of the nitrile hydratase-amidase gene cluster in the photoreactive iron-type nitrile hydratase-producer, rhodococcus sp. n-771. the enzyme showed 96.3%, 77.6%, and 30.4% identities with the oxds of rhodococcus globerulus a-4, pseudomonas chlororaphis b23, and bacillus sp. oxb-1, respectively. the enzyme was expressed in escherichia coli under the control of the lac- or t7 promoters in its intact and his6-tagged forms, pur ... | 2004 | 16233624 |
| the microbial community in a 2,4-dinitrophenol-digesting reactor as revealed by 16s rdna gene analysis. | the microbial community of a 2,4-dinitrophenol-digesting reactor was investigated using different molecular biological techniques based on 16s rdna gene sequences. a pcr-denaturing gradient gel electrophoresis (dgge) analysis of the bacterial community in the reactor showed that one strong and five minor bands were observed in the dgge profile. the results of excising and sequencing dgge bands suggested that members of rhodococcus, nocardioides, and nitrospira species were present in the reactor ... | 2003 | 16233485 |
| isolation of the pseudomonas aeruginosa gene affecting uptake of dibenzothiophene in n-tetradecane. | the dsz desulfurization gene cluster from rhodococcus erythropolis ka2-5-1 was transferred into the chromosomes of pseudomonas aeruginosa strain ncimb9571 using a transposon vector. all of the recombinant strains completely desulfurized 1 mm dibenzothiophene (dbt) in n-tetradecane (n-td) except one, named strain parmi. strain parmi was unable to desulfurize dbt in n-td, but was able to desulfurize it in water. the n-alkane utilization ability, the biosurfactant production and the fatty acid comp ... | 2003 | 16233447 |
| isolation of a recombinant desulfurizing 4,6-diproply dibenzothiophene in n-tetradecane. | rhodococcus erythropolis strain ka2-5-1 is unable to desulfurize 4,6-dipropyl dibenzothiophene (dbt) in the oil phase. the dsz desulfurization gene cluster from r. erythropolis strain ka2-5-1 was transferred into 22 rhodococcal and mycobacterial strains using a transposon-transposase complex. the recombinant strain mr65, from mycobacterium sp. ncimb10403, was able to grow on a minimal medium supplemented with 1.0 mm 4,6-dipropyl dbt in n-tetradecane (50%, v v ) as the sole sulfur source. resting ... | 2003 | 16233419 |
| cloning and characterization of a 4-nitrophenol hydroxylase gene cluster from rhodococcus sp. pn1. | a 4-nitrophenol (4-np)-degrading bacterium was isolated from activated sludge and identified as a rhodococcus sp. this bacterium, designated as strain pn1, could utilize 4-np as a sole carbon, nitrogen and energy source. degradation tests of 4-np using cell suspensions of strain pn1 revealed that the degradation was induced by 4-np and that 4-nitrocatechol (4-nc) was one of the metabolites. a gene library was constructed from the total dna of strain pn1 and introduced into rhodococcus rhodochrou ... | 2003 | 16233382 |
| biodesulfurization of dibenzothiophene with rhodococcus erythropolis atcc 53968 and its mutant in an interface bioreactor. | an interface bioreactor was used in the biodesulfurization (bds) of dibenzothiophene (dbt) with rhodococcus erythropolis atcc 53968. although this strain could efficiently degrade dbt to 2-hydroxybiphenyl (2-hbp), the microbial film peeled off from the surface of the carrier (agar plate) to an organic phase (dodecane or tetradecane). to overcome this problem, a uv mutant, which strongly adhered to the carrier surface, was obtained by a new screening method. the best uv mutant (um-021) could effi ... | 2002 | 16233336 |
| enhancement and stabilization of desulfurization activity of rhodococcus erythropolis ka2-5-1 by feeding ethanol and sulfur components. | we developed a fed-batch culture system fed with ethanol and restricted amounts of sulfur compounds to enhance and stabilize the desulfurizing activity in bacterial cells. in this system using dibenzothiophene (dbt) as the sole sulfur source, a desulfurizing bacterium rhodococcus erythropolis ka2-5-1 cultivated with small amounts of sulfur showed stable desulfurizing activity and a low rate of growth. however, the cells cultured with excess amounts of sulfur showed unstable activity and a high g ... | 2002 | 16233332 |
| difference in degrading p-nitrophenol between indigenous bacteria in a reactor. | p-nitrophenol (pnp) -degrading bacteria were isolated from a reactor using a mineral salt medium containing a low and high pnp concentration. we isolated two bacterial species, pseudomonas sp. ytk17 and rhodococcus opacus ytk32, that utilize pnp as their sole source of carbon and energy. these strains exhibited differences in pnp degradation activity in relation to pnp concentration. strain ytk17 showed a high level of degradation following pre-exposure to a low pnp concentration, whereas strain ... | 2002 | 16233242 |
| diversity of 2,3-dihydroxybiphenyl dioxygenase genes in a strong pcb degrader, rhodococcus sp. strain rha1. | two 2,3-dihydroxybiphenyl (23dhbp) dioxygenase genes, bphc1 and etbc involved in the degradation of polychlorinated biphenyl(s) (pcbs) have been isolated and characterized from a strong pcb degrader, rhodococcus sp. rha1. in this study, four new 23dhbp dioxygenase genes, designated as bphc2, bphc3, bphc4, and bphc5 were isolated from rha1, and their nucleotide sequences were determined. based on amino acid sequence similarities, all of the newly isolated bphc genes could be categorized into type ... | 2002 | 16233225 |
| production of anti-gordonia amarae mycolic acid polyclonal antibody for detection of mycolic acid-containing bacteria in activated sludge foam. | mycolic acid-containing actinomycetes (mycolata) are considered the causative agents of foaming of activated sludge and scum formation in activated sludge treatment plants. in this study, the production of anti-gordonia amarae mycolic acid polyclonal antibodies was investigated. rabbits were immunized with a conjugate of keyhole limpet hemocyanin and mycolic acids of g. amarae, which contained 48 to 56 carbon atoms (average, 52.0). enzyme-linked immunosorbent assay (elisa) demonstrated that the ... | 2001 | 16233121 |
| desulfurization characteristics of thermophilic paenibacillus sp. strain a11-2 against asymmetrically alkylated dibenzothiophenes. | the thermophilic bacterium paenibacillus sp. a11-2, which can utilize dibenzothiophene (dbt) as the sole sulfur source at high temperature (45-55 degrees c), was investigated for its ability to cleave carbon-sulfur bonds in the dibenzothiophene (dbt) ring with asymmetrical alkyl substitution, such as methyl, dimethyl, trimethyl, ethyl and propyl dbts. the biodesulfurization products of each of these alkylated dbts (cx-dbts) were identified and quantitatively determined. the results suggested tha ... | 2001 | 16233084 |
| metabolism of dibenzo-p-dioxin and chlorinated dibenzo-p-dioxin by a gram-positive bacterium, rhodococcus opacus sao101. | a dibenzo-p-dioxin-degrading bacterial strain, rhodococcus opacus sao101, was isolated from forest soil samples collected from the subtropical islands of japan by enrichment of a mineral salt medium containing dibenzofuran as the sole carbon and energy source. the isolated bacterium could utilize dibenzo-p-dioxin as the sole carbon and energy source, and also many monocyclic aromatic compounds, such as toluene, phenol, and chlorobenzene, as well as bicyclic aromatic compounds, such as biphenyl, ... | 2001 | 16233073 |
| selective cleavage of the two cs bonds in asymmetrically alkylated dibenzothiophenes by rhodococcus erythropolis ka2-5-1. | the rhodococcus erythropolis strain ka2-5-1 was characterized by its ability to cleave carbon-sulfur bonds in the dibenzothiophene (dbt) ring by asymmetrically alkyl substitution, such as c2-dbts (e.g., dimethyl and ethyl dbts) and c3-dbts (e.g., trimethyl and propyl dbts), which are known to remain in hydrodesulfurization-treated diesel fuels. after treatment by solid-phase extraction (spe) of solvents from microbial reactions of alkylated dbts (cx-dbts), we used gas chromatography (gc), gc-ato ... | 2001 | 16233063 |
| biodesulfurization of dibenzothiophene and its derivatives through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium bacillus subtilis wu-s2b. | heterocyclic organosulfur compounds such as dibenzothiophene (dbt) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. a moderately thermophilic bacterium bacillus subtilis wu-s2b, which could desulfurize dbt at 50 degrees c through the selective cleavage of carbon-sulfur (cs) bonds, was newly isolated. at 50 degrees c, growing cells of wu-s2b could degrade 0.54 mm dbt within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 ... | 2001 | 16232986 |
| production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by rhodococcus sp. ma 7249. | an attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydrogenase "deficient" strain of rhodococcus (ma 7249). the toluene cis glycol produced was found to have optical rotations of [alpha]d25 = +25.8 (c 0.45, ch3oh) and +72.8 (c 0.42, chcl3) which indicated an absolute configuration of (1s,2r) when compared with previously published values. when cultivated in laboratory fermentor in the presence of tolue ... | 2000 | 16232863 |
| increase in desulfurization activity of rhodococcus erythropolis ka2-5-1 using ethanol feeding. | rhodococcus erythropolis ka2-5-1 is one of the best strains for the desulfurization of dibenzothiophene (dbt) via a sulfur-specific pathway in which dbt is converted to the end product, 2-hydroxybiphenyl, by releasing sulfite via dbt-sulfone and 2-(2'-hydroxyphenyl) benzene sulphinate. the objective of this research is to develop a culture method in order to attain a high cell density with a high level of specific desulfurization activity. compared with glucose or glycerol, ethanol was found to ... | 2000 | 16232759 |
| purification and characterization of dibenzothiophene (dbt) sulfone monooxygenase, an enzyme involved in dbt desulfurization, from rhodococcus erythropolis d-1. | dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, is microbially desulfurized to 2-hydroxybiphenyl by rhodococcus erythropolis d-1. three desulfurization (dsz) enzymes--dszc, a, and b--and flavin reductase are involved in sulfur-specific dbt desulfurization. in this study, dsza was purified, characterized, and crystallized from r. erythropolis d-1. dsza, dbt sulfone monooxygenase, is the second enzyme in microbial dbt desulfurization metabolism and catalyzes the conversion ... | 1999 | 16232672 |
| recognition of a b-cell epitope of the vapa protein of rhodococcus equi in newborn and experimentally infected foals. | the aim of this study was to evaluate the usefulness of the previously identified b-cell epitope tslnlqkdepngrasdtagq of the vapa protein of rhodococcus equi and its association with r. equi pneumonia. a modified peptide designated pn11-14 corresponding to the epitope was recognized by all sera from experimentally infected foals with virulent r. equi atcc103+ containing the virulence plasmid but not by its plasmid-cured derivative atcc103- strain. marked levels of vapa-specific immunoglobulin (i ... | 2005 | 16219093 |
| catalytic mechanism of limonene epoxide hydrolase, a theoretical study. | the catalytic mechanism of limonene epoxide hydrolase (leh) was investigated theoretically using the density functional theory method b3lyp. leh is part of a novel limonene degradation pathway found in rhodococcus erythropolis dcl14, where it catalyzes the hydrolysis of limonene-1,2-epoxide to give limonene-1,2-diol. the recent crystal structure of leh was used to build a model of the leh active site composed of five amino acids and a crystallographically observed water molecule. with this model ... | 2005 | 16218628 |
| [pcb degradation systems in rhodococcus bacteria: multiplex enzyme system determined by multiple isozyme genes]. | 2005 | 16218454 | |
| continuous production of l(+)-tartaric acid from cis-epoxysuccinate using a membrane recycle reactor. | the one-step bioconversion of cis-epoxysuccinate (ces) to l(+)-tartaric acid by dried rhodococcus rhodochrous cells containing ces hydrolase activity was studied by using a continuous bioconversion process. the influence of the ph and the temperature was assessed. a mathematical model was used to quantify the ces hydrolase activity and stability. the optimal ph, which resulted in a maximal ces hydrolase activity and stability, was ph 8.0. a large increase in stability (half-life time) could be o ... | 2006 | 16217652 |
| a lava rock-based biofilter for the treatment of alpha-pinene. | biofiltration is an emerging technology in the united states that utilizes microorganisms to biodegrade harmful contaminants in air to carbon dioxide and water. biofiltration is not only more cost effective, but also more environmentally friendly than traditional technologies such as thermal oxidation and chemical scrubbing. the primary objectives of the study were to operate a lava rock-based laboratory biofiltration system for the removal of alpha-pinene. a consortium of microorganisms to be u ... | 2006 | 16213705 |
| [monitoring of microbial degraders in manned space stations]. | samples of microorganisms from the surface of constructions of mir space station (mir ss) were taken and examined after 13 years of operation. the following microorganisms were isolated and identified: 12 fungal species belonging to the genera penicillium, aspergillus, cladosporium, and aureobasidium; 3 yeast species belonging to the genera debaryomyces, candida, and rhodotorula; and 4 bacterial species belonging to the genera bacillus, myxococcus, and rhodococcus. the predominant species in all ... | 2005 | 16212041 |
| [the role of intercellular contacts in the initiation of growth and in the development of a transiently nonculturable state by the cultures of rhodococcus rhodochrous grown in poor media]. | it was found that the growth of rhodococcus rhodochrous cells in modified saton's medium strongly depends on the rate of culture agitation in the flask: an agitation at 250 rpm in flasks with baffles stops cell multiplication, whereas slight agitation leads to pronounced culture growth. the growth retardation phenomenon was reversible and did not manifest itself in exponential-phase cultures or when the cells were grown in a rich medium; furthermore, it was not connected with the degree of cultu ... | 2005 | 16211852 |
| characterization of gordonia sp. strain f.5.25.8 capable of dibenzothiophene desulfurization and carbazole utilization. | a dibenzothiophene (dbt)-degrading bacterial strain able to utilize carbazole as the only source of nitrogen was identified as gordonia sp. f.5.25.8 due to its 16s rrna gene sequence and phenotypic characteristics. gas chromatography (gc) and gc-mass spectroscopy analyses showed that strain f.5.25.8 transformed dbt into 2-hydroxybiphenyl (2-hbp). this strain was also able to grow using various organic sulfur or nitrogen compounds as the sole sulfur or nitrogen sources. resting-cell studies indic ... | 2006 | 16211383 |
| fatal pulmonary infection associated with a novel organism, "para-streptomyces abscessus". | actinomycetes are increasingly recognized as pathogenic in the immunocompromised host. we isolated an asporogenous, nonmotile, aerobic gram-positive rod from a transplant recipient with a fatal pulmonary infection. the pathology was similar to that associated with rhodococcus equi, including intra-histiocytic localization. the organism was relatively inert in standard biochemical tests. 16s rrna gene sequencing indicated a potentially unique organism most closely related to the genus streptomyce ... | 2005 | 16208023 |
| n-acylhomoserine lactone quorum-sensing molecules are modified and degraded by rhodococcus erythropolis w2 by both amidolytic and novel oxidoreductase activities. | the rhodococcus erythropolis strain w2 has been shown previously to degrade the n-acylhomoserine lactone (ahl) quorum-sensing signal molecule n-hexanoyl-l-homoserine lactone, produced by other bacteria. data presented here indicate that this gram-positive bacterium is also capable of using various ahls as the sole carbon and energy source. the enzymic activities responsible for ahl inactivation were investigated in r. erythropolis cell extracts and in whole cells. r. erythropolis cells rapidly d ... | 2005 | 16207914 |
| association of pneumonia in foals caused by rhodococcus equi with farm soil geochemistry. | to quantify and compare geochemical factors in surface soils from horse-breeding farms with horses with pneumonia caused by rhodococcus equi (affected farms) and horse-breeding farms with no history of pneumonia caused by r equi (unaffected farms). | 2002 | 16206788 |
| role of the ssu and seu genes of corynebacterium glutamicum atcc 13032 in utilization of sulfonates and sulfonate esters as sulfur sources. | corynebacterium glutamicum atcc 13032 was found to be able to utilize a broad range of sulfonates and sulfonate esters as sulfur sources. the two gene clusters potentially involved in sulfonate utilization, ssud1cba and ssui-seuabc-ssud2, were identified in the genome of c. glutamicum atcc 13032 by similarity searches. while the ssu genes encode proteins resembling ssu proteins from escherichia coli or bacillus subtilis, the seu gene products exhibited similarity to the dibenzothiophene-degradin ... | 2005 | 16204527 |
| characteristics of styrene degradation by rhodococcus pyridinovorans isolated from a biofilter. | a novel strain (pyj-1) of rhodococcus pyridinovorans that was isolated from a biofilter was able to degrade styrene at a maximum rate of 0.16 mg (mg protein)(-1) h(-1) in batch culture at 97 mg l(-1) of initial styrene gas concentration. the optimum ph and temperature for styrene degradation were 7 and 32 degrees c, respectively. the degradation kinetic constants were obtained using substrate inhibition kinetics. in a perlite-packed biofilter the maximum styrene removal rate by the strain was 27 ... | 2005 | 16202798 |
| functional expression system for cytochrome p450 genes using the reductase domain of self-sufficient p450rhf from rhodococcus sp. ncimb 9784. | cytochrome p450rhf from rhodococcus sp. ncimb 9784 is a self-sufficient p450 monooxygenase. we report here a simple system for the functional expression of various p450 genes using the reductase domain of this p450rhf, which comprises flavin mononucleotide- and nicotinamide adenine dinucleotide phosphate binding motifs and a [2fe2s] ferredoxin-like center. vector pred was constructed, which carried the t7 promoter, cloning sites for a p450, a linker sequence, and the p450rhf reductase domain, in ... | 2006 | 16195793 |
| sorption of hydrophobic organic chemicals to bacteria. | the toxicity and time-dependent sorption of three hydrophobic organic chemicals to rhodococcus rhodochrous bacteria were investigated. in experiments, environmentally relevant concentrations of pentachlorophenol (pcp), hexachlorobenzene (hcb), and dichlorobiphenyl (dpcb) were applied to living (both growing and nongrowing) bacteria as well as to dead bacteria. for pcp (an ionizing chemical), bacterial growth decreased, and bacterial death increased, as the pcp concentration increased. in sorptio ... | 2005 | 16193738 |