Publications

sexually transmitted infections, benign prostatic hyperplasia and lower urinary tract symptom-related outcomes: results from the prostate, lung, colorectal and ovarian cancer screening trial.	to examine whether a history of sexually transmitted infections (stis) or positive sti serology is associated with prevalent and incident benign prostatic hyperplasia (bph)/lower urinary tract symptoms (luts)-related outcomes in the prostate, lung, colorectal and ovarian cancer screening trial.	2015	25601300
a multiplex pcr assay for the simultaneous detection of chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis.	for developing countries, sexually transmitted infections (stis) and their complications are ranked in the top 5 disease categories for which adults seek medical treatment. chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), and trichomonas vaginalis (tv) are the three most common stis worldwide, with tv accounting for over half of the cases. in developing countries, traditional methods for diagnosing stis are laborious, often not very sensitive, and have a long turnaround time with most rec ...	2015	25595915
pathogenesis of fallopian tube damage caused by chlamydia trachomatis infections.	chlamydia trachomatis is the leading cause of bacterial sexually transmitted disease worldwide resulting in 4-5 million new cases of chlamydia annually and an estimated 100 million cases per annum. infections of the lower female genital tract (fgt) frequently are asymptomatic; thus, they often remain undiagnosed or untreated. if infections are either not resolved or left untreated, chlamydia can ascend to the upper fgt and infect the fallopian tubes (fts) causing salpingitis that may lead to fun ...	2015	25592078
uptake and population-level impact of expedited partner therapy (ept) on chlamydia trachomatis and neisseria gonorrhoeae: the washington state community-level randomized trial of ept.	expedited partner therapy (ept), the practice of treating the sex partners of persons with sexually transmitted infections without their medical evaluation, increases partner treatment and decreases gonorrhea and chlamydia reinfection rates. we conducted a stepped-wedge, community-level randomized trial to determine whether a public health intervention promoting ept could increase its use and decrease chlamydia test positivity and gonorrhea incidence in women.	2015	25590331
doxycycline prophylaxis to reduce incident syphilis among hiv-infected men who have sex with men who continue to engage in high-risk sex: a randomized, controlled pilot study.	incident syphilis infections continue to be especially prevalent among a core group of hiv-infected men who have sex with men (msm). because of synergy between syphilis and hiv infections, innovative means for controlling incident syphilis infections are needed.	2015	25585069
risk factors for hiv and sti diagnosis in a community-based hiv/sti testing and counselling site for men having sex with men (msm) in a large german city in 2011-2012.	in recent years community-based voluntary counselling and testing sites (cb-vct) for men having sex with men (msm) have been established in larger cities in germany to offer more opportunities for hiv testing. increasingly, cb-vcts also offer testing for other bacterial sexually transmitted infections. in hamburg, tests in cb-vcts are offered free and anonymously. data on demographics and sexual risk behaviours are collected with a paper questionnaire.	2015	25582975
etiology and antimicrobial susceptibility of pathogens responsible for urethral discharge among men in harare, zimbabwe.	periodic etiological surveillance of sexually transmitted infection (sti) syndromes is required to validate treatment algorithms used to control stis. however, such surveys have not been performed in zimbabwe over the past decade.	2014	25581806
a review of current practices to increase chlamydia screening in the community--a consumer-centred social marketing perspective.	chlamydia trachomatis is one of the most frequently reported sexually transmitted infections (sti) in australia, the uk and europe. yet, rates of screening for stis remain low, especially in younger adults.	2016	25580560
recent advances in understanding and managing chlamydia trachomatis infections.	worldwide, chlamydia trachomatis infections rank among the most common sexually transmitted infections (sti), and cause notable reproductive morbidity in women. although advances in highly accurate and non-invasive diagnostic testing have allowed for better estimation of the burden of disease-especially the asymptomatic state-we still lack a true point-of-care test, and many infections go undetected and untreated. moreover, limited resources and effort for managing sexual partners of those in wh ...	2014	25580274
treatment of chlamydial infections: 2014 update.	chlamydiae are obligate intracellular bacterial pathogens whose entry into mucosal epithelial cells is required for intracellular survival and subsequent growth. the life cycle of chlamydia spp. and the ability to cause persistent, often subclinical infection, has major ramifications for diagnosis and treatment of chlamydia trachomatis and c. pneumoniae infections in humans.	2015	25579069
carrot cells: a pioneering platform for biopharmaceuticals production.	carrot (daucus carota l.) is of importance in the molecular farming field as it constitutes the first plant species approved to produce biopharmaceuticals for human use. in this review, features that make carrot an advantageous species in the molecular farming field are analyzed and a description of the developments achieved with this crop thus far is presented. a guide for genetic transformation procedures is also included. the state of the art comprises ten vaccine prototypes against measles v ...	2015	25572939
contribution of il-12a and il-12b polymorphisms to chlamydia trachomatis-specific cell-mediated immune responses.	inherited variance in the il-12b gene is associated with susceptibility to chlamydia trachomatis-induced tubal factor infertility and disease severity. in this study, our aim was to discover how polymorphisms in il-12-coding genes influence c. trachomatis-induced immune responses and il-12 production. the study population consisted of 240 women. il-12a and il-12b single nucleotide polymorphisms (snps) were determined from isolated dna using the sequenom system with matrix-assisted laser desorpti ...	2015	25565213
chlamydia trachomatis infection: screening and management.	to review current criteria and rationale for chlamydia trachomatis screening, testing methods, and treatment of infection.	2014	25554725
tripolar mitosis in human cells and embryos: occurrence, pathophysiology and medical implications.	tripolar mitosis is a specific case of cell division driven by typical molecular mechanisms of mitosis, but resulting in three daughter cells instead of the usual count of two. other variants of multipolar mitosis show even more mitotic poles and are relatively rare. in nature, this phenomenon was frequently observed or suspected in multiple common cancers, infected cells, the placenta, and in early human embryos with impaired pregnancy-yielding potential. artificial causes include radiation and ...	2015	25554607
highly sensitive and novel point-of-care system, aqcare chlamydia trf kit for detecting chlamydia trachomatis by using europium (eu) (iii) chelated nanoparticles.	the bacterium chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. since no simple and effective tool exists to diagnose c. trachomatis infections, we evaluated a novel point-of-care (poc) test, aqcare chlamydia trf kit, which uses europium-chelated nanoparticles and a time-resolved fluorescence reader.	2015	25553280
a 2-pyridone-amide inhibitor targets the glucose metabolism pathway of chlamydia trachomatis.	in a screen for compounds that inhibit infectivity of the obligate intracellular pathogen chlamydia trachomatis, we identified the 2-pyridone amide ksk120. a fluorescent ksk120 analogue was synthesized and observed to be associated with the c. trachomatis surface, suggesting that its target is bacterial. we isolated ksk120-resistant strains and determined that several resistance mutations are in genes that affect the uptake and use of glucose-6-phosphate (g-6p). consistent with an effect on g-6p ...	2014	25550323
[lymphogranuloma venereum: the first hungarian cases].	lymphogranuloma venereum is a sexually transmitted infection caused by the chlamydia trachomatis serovars l1-3. it has been found to be endemic in tropical countries. in the last decades several cases have been reported in western europe, particularly in men who have sex with men population infected with human immunodeficiency virus. the authors present three cases of lymphogranuloma venereum infections, observed at their department in 2013 and 2014. the three human immunodeficiency virus infect ...	2015	25544054
[genitourethral infections caused by d-k serotypes of chlamydia trachomatis].	sexually transmitted infections of the urogenital tract are most commonly caused by the intracellular bacteria chlamydia trachomatis worldwide, resulting the clinical picture of acute urethritis in men as well as urethritis and endocervicitis in women. as women often present with few symptoms only or a completely symptom-free disease course, one of the most important long-term complications is chronic pelvic inflammatory disease often followed by the development of infertility caused by chronic ...	2015	25544050
[sexually transmitted coinfections. hiv coinfections].	coinfections of sexually transmitted infections are frequent due to the same transmission routes which may facilitate the transmission of other sexually transmitted infections. sexually transmitted coinfections are associated with atypical and generally more severe clinical features, more complications, resistency to treatment, unfavourable outcome, and worse prognosis. sexually transmitted infections may increase the likelihood of acquiring and transmission of hiv infection. the authors summari ...	2015	25544048
role of chlamydia trachomatis and emerging chlamydia-related bacteria in ectopic pregnancy in vietnam.	in this case-control study, we investigated the seroprevalence and molecular evidence of chlamydia trachomatis and waddlia chondrophila in ectopic pregnancies (ep) and uneventful control pregnancies in 343 women from vietnam. whereas presence of c. trachomatis igg was strongly associated with ep [adjusted odds ratio (aor) 5·41, 95% confidence interval (ci) 2·58-11·32], its dna remained undetected in all tubal lesions. we confirmed an independent association between antibodies against waddlia and ...	2015	25543825
acute epididymitis revisited: impact of molecular diagnostics on etiology and contemporary guideline recommendations.	acute epididymitis is a common infectious disease of unknown etiology in about 30% of cases with guidelines based on studies published >15 yr ago.	2015	25542628
[expression of gamma interferon during hpv and chlamydia trachomatis infection in cervical samples].	the aim of this study was to mesure the expression of gamma interferon in hpv and chlamydia trachomatis infection in squamous intraepithelial lesions.	2015	25541008
stability of chlamydia trachomatis on storage of dry swabs for accurate detection by nucleic acid amplification tests.		2015	25540399
assessment of coinfection of sexually transmitted pathogen microbes by use of the anyplex ii sti-7 molecular kit.	anyplex sti-7 is a new molecular kit that detects seven sexually transmitted pathogens. among 202 subjects screened for genital infection, 143 (70.4%) were diagnosed with at least one pathogen, in concordance with reference methods. in addition, the anyplex sti-7 demonstrated coinfections, such as that with ureaplasma parvum and chlamydia trachomatis, in young women.	2014	25540390
three cases of reactive arthritis secondary to lymphogranuloma venereum.	we describe 3 cases of sexually acquired reactive arthritis following rectal lymphogranuloma venereum (lgv) infection in london men who have sex with men. while sexually acquired reactive arthritis is well recognized following urogenital chlamydia trachomatis infections, the association with rectal infection by lgv-associated serovars of c. trachomatis has been seldom reported, and thus the organism might not be sought by clinicians. the recognition of the various clinical syndromes of lgv by al ...	2015	25539432
[chlamydia trachomaatis dna in leukocytes of peripheral blood from neonates].	diagnosis of chlamydia trachomatis infection in newborns is difficult; however, this diagnosis is performed by cell culture or by detection of igm antibodies against c. trachomatis. detection of c. trachomatis dna in peripheral blood leukocytes using polymer chain reaction (pcr) may be a better tool for the diagnosis of infection by this pathogen.	2017	25533744
performance evaluation of a dna chip assay in the identification of major genitourinary pathogens.	to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections.	2015	25533217
interventions for the treatment of trachoma: an overview of cochrane systematic reviews.	trachoma is an infectious disease caused by the bacterium chlamydia trachomatis. infection with c. trachomatis produces characteristic changes to the inner surface of the eyelids, resulting in sequela that when left untreated, leads to vision impairment and blindness. repeated trachoma infections can cause severe scarring of the inside of the eyelid and can cause the eyelashes to scratch the cornea (trichiasis). the objective of this overview was to synthesize the evidence from cochrane systemat ...	2014	25532886
sexually transmitted diseases: epidemiological and clinical aspects in adults.	sexually transmitted diseases (stds) are the first 10 causes of unpleased diseases in young adult women in the world. the concept of stds includes a series of syndromes caused by pathogens that can be acquired by sexual intercourse or sexual activity.adolescents and young adults are responsible for only 25% of the sexually active population and they represent almost 50% of all newly acquired stds.in this way, we evaluated the epidemiological and clinical aspects of most relevant pathogens as nei ...	2016	25532465
the potential role of nemonoxacin for treatment of common infections.	nemonoxacin , a novel non-ﬂuorinated quinolone, exhibits potent activity against gram-positive bacteria, including mrsa and fluoroquinolone-resistant mrsa, gram-negative and atypical pathogens. this agent also has a reduced propensity for resistance development in many kinds of pathogens.	2015	25529577
chlamydia pneumoniae effector chlamydial outer protein n sequesters fructose bisphosphate aldolase a, providing a benefit to bacterial growth.	pathogenic chlamydiae are obligate intracellular pathogens and have adapted successfully to human cells, causing sexually transmitted diseases or pneumonia. chlamydial outer protein n (copn) is likely a critical effector protein secreted by the type iii secretion system in chlamydiae, which manipulates host cells. however, the mechanisms of its action remain to be clarified. in this work, we aimed to identify previously unidentified copn effector target in host cells.	2014	25528659
barriers to and facilitators of partner notification for chlamydia trachomatis among health care professionals.	partner notification (pn) is an essential case-finding tool in the management of sexually transmitted infections (stis). yet, data on the effectiveness and factors impacting implementation of pn in the netherlands are lacking. with the aim of further exploring and improving the pn process, the current study assessed perceived barriers and facilitators among health care professionals in the sti clinical setting. in particular, we explored the management of pn in young heterosexual patients diagno ...	2014	25526679
screening adolescents for sexually transmitted infections in the pediatric emergency department.	the objectives of this study were to determine the prevalence of chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) infections among adolescents presenting to a pediatric emergency department (ped), to assess the association between these infections and certain risk factors, and to assess the feasibility of routine screening for sexually transmitted infections (stis) in the ped.	2015	25526018
the sportsmart study: a pilot randomised controlled trial of sexually transmitted infection screening interventions targeting men in football club settings.	uptake of chlamydia screening by men in england has been substantially lower than by women. non-traditional settings such as sports clubs offer opportunities to widen access. involving people who are not medically trained to promote screening could optimise acceptability.	2014	25512674
confirmatory assays are essential when using molecular testing for neisseria gonorrhoeae in low-prevalence settings: insights from the third national survey of sexual attitudes and lifestyles (natsal-3).	to investigate the occurrence of unconfirmed positive gonorrhoea results when using molecular testing within a large population-based survey.	2014	25512673
[chlamydia: from population screening to individual repeated screening].	chlamydia trachomatis is a frequent sexually transmitted infection especially in young adults and adolescents. its complications can impair a woman's reproductive potential. chlamydia control has several challenges. these include asymptomatic infections; a long duration of untreated infections; re-infections and partner treatments. any person with infection is at high risk of re-infection. repeated screening would decrease, at an individual level, the risk of complications. general practitioners ...	2014	25507087
interferon-gamma gene polymorphism influences the frequency of a chlamydia trachomatis cervical infection in young women.	cervicitis associated with chlamydia trachomatis is frequent worldwide, but the factors determining susceptibility to infection remain incompletely determined. we evaluated whether a functional single nucleotide polymorphism at position +874 in the gene coding for interferon gamma (rs2430561) influenced the likelihood of having a cervical c. trachomatis infection. this was a cross-sectional study of 142 sexually-active women attending a general gynaecology service on the outskirts of the city of ...	2015	25505046
high prevalence and incidence of asymptomatic sexually transmitted infections during pregnancy and postdelivery in kwazulu natal, south africa.	we report the prevalence and incidence of 3 treatable sexually transmitted pathogens (neiserria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis) in women who were hiv infected or at high risk for hiv infection, in pregnancy and postpartum, respectively.	2015	25504300
diagnosis of common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr.	urethritis is one of the most important causes of morbidity and mortality in developing countries. the aim of this study was to detect common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr.185 male patients who presented at the skin and venereal clinic of the dhaka medical college, bangladesh with clinical symptoms suggestive of urethritis were enrolled in this study. urethral discharges were tested for detection of neisseria gonorrhoeae by gram stain, culture and ...	2014	25500516
chlamydia trachomatis infection: possible cofactor for oropharyngeal cancer development?		2015	25500096
detection of chlamydia trachomatis infection in patients seen at a sexually transmitted infection clinic.		2015	25498158
evaluation of pooled ocular and vaginal swabs by the cepheid genexpert ct/ng assay for the detection of chlamydia trachomatis and neisseria gonorrhoeae compared to the genprobe aptima combo 2 assay.	ocular swabs from tanzania were tested for chlamydia trachomatis (ct), and self-collected vaginal swabs collected through a home collection program, iwantthekit.org, were tested for neisseria gonorrhoeae and ct to evaluate cepheid genexpert for the use of pooling multiple specimens before testing. genexpert shows to be a promising test for pooling.	2014	25497459
genotyping of endocervical chlamydia trachomatis strains and detection of serological markers of acute and chronic inflammation in their host.	chlamydia trachomatis (c. trachomatis) is the most prevalent cause of bacterial sexually transmitted infections (sti) recognized throughout the world. the aim of this study is to determine different genotypes of genital c. trachomatis and the association between the serological markers of inflammation and genotypes of c. trachomatis in sexually active women (n=80) attending shahid beheshti hospital in isfahan, iran.	2012	25493166
the epidemiology of chlamydia trachomatis organism load during genital infection: a systematic review.	the role of organism load in chlamydia trachomatis infection is not well understood. we conducted a systematic review to investigate the epidemiology of c. trachomatis organism load in human genital chlamydia infection.	2015	25492913
a randomized controlled trial of the effects of a brief intervention to increase chlamydia and gonorrhea testing uptake among young adult female emergency department patients.	the objective of this study was to test the effect of a brief educational and counseling intervention on increasing the uptake of free testing for chlamydia trachomatis (chlamydia) and neisseria gonorrhea (gonorrhea) among young female emergency department (ed) patients. women are particularly vulnerable to more serious consequences of these infections due to asymptomatic presentation. increased testing is important to detect, treat, and halt the spread of these infections among asymptomatic wom ...	2014	25491714
high-resolution typing of chlamydia trachomatis: epidemiological and clinical uses.	a state-of-the-art overview of molecular chlamydia trachomatis typing methods that are used for routine diagnostics and scientific studies.	2015	25490105
nucleic acid contamination in sexual health clinics.	this review explores the potential for nucleic acid contamination with gonorrhoea or chlamydia within healthcare settings, the implications that this has for the transmission of infection and the potential for contamination to cause false positive test results in patients.	2015	25485648
the conjunctival microbiome in health and trachomatous disease: a case control study.	trachoma, caused by chlamydia trachomatis, remains the world's leading infectious cause of blindness. repeated ocular infection during childhood leads to scarring of the conjunctiva, in-turning of the eyelashes (trichiasis) and corneal opacity in later life. there is a growing body of evidence to suggest non-chlamydial bacteria are associated with clinical signs of trachoma, independent of c. trachomatis infection.	2014	25484919
portal hypertension and an atypical reactive arthritis like presentation in a patient infected with hepatitis c virus genotype 3.	reactive arthritis (rea) is defined as a peripheral arthritis lasting longer than 1 month, associated with urethritis, cervicitis, or diarrhea. the reported annual incidence of rea is approximately 30-40 cases per 100,000 adults, occurring commonly in the age group of 16 and 35 years. it is known to be associated with gastrointestinal infections with shigella, salmonella, and campylobacter species and other microorganisms, as well as with genitourinary infections (especially with chlamydia trach ...	2014	25484389
immunity, immunopathology, and human vaccine development against sexually transmitted chlamydia trachomatis.	this review examines the immunity, immunopathology, and contemporary problems of vaccine development against sexually transmitted chlamydia trachomatis. despite improved surveillance and treatment initiatives, the incidence of c. trachomatis infection has increased dramatically over the past 30 years in both the developed and developing world. studies in animal models have shown that protective immunity to c. trachomatis is largely mediated by th1 t cells producing ifn-γ which is needed to preve ...	2014	25483666
chlamydia trachomatis prevalence and chlamydial/hpv co-infection among hpv-unvaccinated young italian females with normal cytology.	infections caused by chlamydia trachomatis (ct) and human papillomavirus (hpv) are the two main sexually transmitted infections; however, epidemiological data on ct prevalence and ct/hpv co-infection in italy are scant. this study aimed at estimating the prevalence of ct infection and ct/hpv co-infection in young hpv-unvaccinated females with normal cytology, and placed particular attention on the possible association between ct-dna positivity and different hpv infecting genotypes. five hundred ...	2015	25483545
infectious agents are associated with psychiatric diseases.	there are several infectious agents in the environment that can cause persistent infections in the host. they usually cause their symptoms shortly after first infection and later persist as silent viruses and bacteria within the body. however, these chronic infections may play an important role in the pathogenesis of schizophrenia and tourette's syndrome (ts). we investigated the distribution of different neurotrophic infectious agents in ts, schizophrenia and controls. a total of 93 individuals ...	2012	25478103
chlamydia muridarum infection-induced destruction of male germ cells and sertoli cells is partially prevented by chlamydia major outer membrane protein-specific immune cd4 cells.	chlamydia trachomatis infections are increasingly prevalent worldwide. male chlamydial infections are associated with urethritis, epididymitis, and orchitis; however, the role of chlamydia in prostatitis and male factor infertility remains controversial. using a model of chlamydia muridarum infection in male c57bl/6 mice, we investigated the effects of chlamydial infection on spermatogenesis and determined the potential of immune t cells to prevent infection-induced outcomes. antigen-specific cd ...	2015	25472923
risk factors and clinical features of ovarian pregnancy: a case-control study.	to identify risk factors for ovarian pregnancy (op) and compare clinical features between op and tubal pregnancy (tp) patients.	2014	25472658
investigating the epidemiology of repeat chlamydia trachomatis detection after treatment by using c. trachomatis ompa genotyping.	repeat chlamydia trachomatis detection frequently occurs within months after c. trachomatis infection treatment. the origins of such infection (persistence versus reinfection from untreated or new partners) are varied and difficult to determine. c. trachomatis strains can be differentiated by sequencing the ompa gene encoding the outer membrane protein a (ompa). we used ompa genotyping to investigate the epidemiology of repeat c. trachomatis detection after treatment in c. trachomatis-infected s ...	2015	25472488
activation of epidermal growth factor receptor is required for chlamydia trachomatis development.	chlamydia trachomatis (c. trachomatis) is a clinically significant human pathogen and one of the leading causative agents of sexually transmitted diseases. as obligate intracellular bacteria, c. trachomatis has evolved strategies to redirect the host's signaling and resources for its own survival and propagation. despite the clinical notoriety of chlamydia infections, the molecular interactions between c. trachomatis and its host cell proteins remain elusive.	2014	25471819
occurrence of chlamydia trachomatis in military environment on the example of professional soldiers in the polish armed forces.	this article presents the results of a preliminary study concerning cases of chlamydia trachomatis infections among professional soldiers in the polish armed forces. soldiers who declared casual sexual contact with women were investigated in this study regarding the transmission of chlamydial infections by sexual activity. in total, 66 healthy, sexually active professional polish soldiers, aged between 27 and 44, who didn't report any symptoms of urogenital infection were investigated. urine sam ...	2014	25471163
activation of the nlrp3 inflammasome by vault nanoparticles expressing a chlamydial epitope.	the full potential of vaccines relies on development of effective delivery systems and adjuvants and is critical for development of successful vaccine candidates. we have shown that recombinant vaults engineered to encapsulate microbial epitopes are highly stable structures and are an ideal vaccine vehicle for epitope delivery which does not require the inclusion of an adjuvant. we studied the ability of vaults which were engineered for use as a vaccine containing an immunogenic epitope of chlam ...	2015	25448112
the clinical characteristics and genotype distribution of chlamydia trachomatis infection in infants less than six months of age hospitalized with pneumonia.	chlamydia trachomatis is a common sexually-transmitted bacterial pathogen. as no routine screening is performed during pregnancy, neonates and infants are at high risk for c. trachomatis infection. the objective of this study was to investigate the morbidity, clinical characteristics and genotype distribution of c. trachomatis pneumonia in infants less than six months of age.	2015	25445657
detection of bacteria and analyses of chlamydia trachomatis viability in patients with postvenereal reactive arthritis.	postvenereal reactive arthritis is an inflammatory form of arthritis that commonly develops after urogenital infection, predominantly in human leucocyte antigen-b27-positive men in the third decade of life. in our hospital, patients underwent synovectomy before a 4-month course of antibiotics (ciprofloxacin, tetracycline and roxithromicin). the clinical remission was achieved in approximately 70% patients. at molecular level, the remission was associated with the negative polymerase chain reacti ...	2014	25442760
the verification of nucleic acid amplification testing (gen-probe aptima assay) for chlamydia trachomatis from ocular samples.	chlamydia trachomatis conjunctivitis may present with extended symptoms, and it can have social ramifications as a sexually transmitted disease. for appropriate therapy, c. trachomatis conjunctivitis should be diagnosed definitively. this study presents the verification of nucleic acid amplification testing (naat; gen-probe aptima combo 2 assay) for detection of c. trachomatis ribosomal rna (rrna) from direct ocular samples.	2015	25439605
biological and behavioral risks for incident chlamydia trachomatis infection in a prospective cohort.	to identify biological and behavioral risks for incident chlamydia trachomatis among a prospective cohort of young women followed frequently.	2014	25437724
tumor suppressor p53 alters host cell metabolism to limit chlamydia trachomatis infection.	obligate intracellular bacteria depend entirely on nutrients from the host cell for their reproduction. here, we show that obligate intracellular chlamydia downregulate the central tumor suppressor p53 in human cells. this reduction of p53 levels is mediated by the pi3k-akt signaling pathway, activation of hdm2, and subsequent proteasomal degradation of p53. the stabilization of p53 in human cells severely impaired chlamydial development and caused the loss of infectious particle formation. dna- ...	2014	25437549
evaluation of extragenital screening for gonorrhea and chlamydia in hiv-infected active duty air force members.	this study evaluated the hypothesis that detection of neisseria gonorrhoeae (gc) and chlamydia trachomatis (ct) infections among hiv-infected active duty members of the u.s. air force would increase after expanding screening to include extragenital infections. before and after the start of extragenital screening, urethral screening was positive for gc/ct in 2.9% and 1.9% of hiv-infected service members. much higher proportions of rectal (11.1%) and pharyngeal (21.9%) specimens were found to be p ...	2014	25436876
a field evaluation of a new molecular-based point-of-care test for chlamydia and gonorrhoea in remote aboriginal health services in australia.	background point-of-care (poc) tests could be important public health tools in settings with treatment delays and high rates of sexually transmissible infections (stis). use is limited due to suboptimal performance. the performance and ease-of-use of a new molecular-based poc test for simultaneous detection of chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) was assessed, alongside two single-organism immunochromatographic tests (ict).	2015	25426655
an emerging treatment: topical ivermectin for papulopustular rosacea.	ivermectin shows broad-spectrum anti-parasitic activity. it kills the demodex mites that reside in the pilosebaceous units of patients with papulopustular rosacea. ivermectin also has anti-inflammatory effects, it decreases cellular and humoral immune responses. inflammatory mechanisms appear to play a dominant role in the development of rosacea inflammatory lesions. additionally, there is some evidence that it shows antimicrobial activity against myobacterium tuberculosis and chlamydia trachoma ...	2015	25424053
progress in genotyping of chlamydia trachomatis.	to review the common genotyping techniques of chlamydia trachomatis in terms of their principles, characteristics, applications and limitations.	2014	25421201
an autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines.	the autotransporter pathway, ubiquitous in gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. capitalizing on its crystal structure, we have engineered the escherichia coli autotransporter hemoglobin protease (hbp) into a versatile platform for secretion and surface display of multiple heterologous proteins in one carrier molecule.	2014	25421093
impact of vaginal-rectal ultrasound examinations with covered and low-level disinfected transducers on infectious transmissions in france.	the risk of cross-infection from shared ultrasound probes in endorectal and vaginal ultrasonography due to low-level disinfection (lld) is difficult to estimate because potential infections are also sexually transmitted diseases, and route of contamination is often difficult to establish. in france, the widely used standard for prevention of infections is through the use of probe covers and lld of the ultrasound transducer by disinfectant wipes. we performed an in silico simulation based on a sy ...	2014	25419772
[chlamydia: from population screening to individual repeated screening].	chlamydia trachomatis is a frequent sexually transmitted infection especially in young adults and adolescents. its complications can impair a woman's reproductive potential. chlamydia control has several challenges. these include asymptomatic infections; a long duration of untreated infections; re-infections and partner treatments. any person with infection is at high risk of re-infection. repeated screening would decrease, at an individual level, the risk of complications. general practitioners ...	2014	25417359
host nectin-1 is required for efficient chlamydia trachomatis serovar e development.	interaction of herpes simplex virus (hsv) glycoprotein d (gd) with the host cell surface during chlamydia trachomatis/hsv co-infection stimulates chlamydiae to become persistent. during viral entry, gd interacts with one of 4 host co-receptors: hvem (herpes virus entry mediator), nectin-1, nectin-2 and 3-o-sulfated heparan sulfate. hvem and nectin-1 are high-affinity entry receptors for both hsv-1 and hsv-2. nectin-2 mediates hsv-2 entry but is inactive for hsv-1, while 3-o-sulfated heparan sulf ...	2014	25414835
[serological evidence of infection by three species of chlamydia in pregnant women in mexico].	background: chlamydiales are obligate intracellular pathogens causing a wide range of diseases in humans and animals.	2014	25412551
[opposite trends in the incidence of gonorrhea and syphilis in the east bohemian region - 30 years of surveillance].	the epidemiology of selected sexually transmitted diseases in the czech republic has been carefully evaluated for many years. data from 1981-2011 for eastern bohemia shows a sharp decrease in the incidence of gonorrhea in 1993-1994 and a very low incidence thereafter with a slightly higher prevalence in males. however, syphilis and genitourinary infections with chlamydia trachomatis show entirely opposite trends. also, for the similar number of diagnostic tests performed, chlamydia had a 10 fold ...	2014	25412486
transcriptional profiling of human epithelial cells infected with plasmid-bearing and plasmid-deficient chlamydia trachomatis.	chlamydia trachomatis is an obligate intracellular epitheliotropic bacterial pathogen of humans. infection of the eye can result in trachoma, the leading cause of preventable blindness in the world. the pathophysiology of blinding trachoma is driven by multiple episodes of reinfection of conjunctival epithelial cells, producing an intense chronic inflammatory response resulting in submucosal tissue remodeling and scarring. recent reports have shown that infection with trachoma organisms lacking ...	2015	25404022
the added value of chlamydia screening between 2008-2010 in reaching young people in addition to chlamydia testing in regular care; an observational study.	internet-based chlamydia screening implementation (chlamydia screening programme) was introduced in the netherlands in 2008-2010 to detect and treat asymptomatic infections and to limit ongoing transmission through annual testing and treatment of chlamydia trachomatis in young people (16-29 years). this population-based screening may be less effective when addressing individuals who are already covered by regular care, instead of addressing a hidden key population without chlamydia testing exper ...	2014	25403312
should screening for chlamydia trachomatis and neisseria gonorrhoeae in hiv-men who have sex with men be recommended?	sexually transmitted infections (sti) like chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) have been associated with increased risk of hiv acquisition (1). it has been also described as a high prevalence of asymptomatic ct and ng infections in men who have sex with men (msm) (2). the aim of this study was to know the prevalence of ct and/or ng infections in asymptomatic hiv-msm and the related factors.	2014	25397411
ongoing epidemic of lymphogranuloma venereum in hiv-positive men who have sex with men: how symptoms should guide treatment.	lymphogranuloma venereum (lgv) is a sexually transmitted infection (sti) caused by chlamydia trachomatis (ct) genotype l (l1, l2 and l3). recent outbreaks of lgv in europe and north america affected mainly men who have sex with men (msm). infections with ct serotypes d-k are mostly associated with mild symptoms or may be clinically silent. however, infections with l genotypes are more invasive and induce anogenital ulcer or inguinal lymphadenopathy.	2014	25394161
whole-genome enrichment and sequencing of chlamydia trachomatis directly from clinical samples.	chlamydia trachomatis is a pathogen of worldwide importance, causing more than 100 million cases of sexually transmitted infections annually. whole-genome sequencing is a powerful high resolution tool that can be used to generate accurate data on bacterial population structure, phylogeography and mutations associated with antimicrobial resistance. the objective of this study was to perform whole-genome enrichment and sequencing of c. trachomatis directly from clinical samples.	2014	25388670
the role of the immune response in chlamydia trachomatis infection of the male genital tract: a double-edged sword.	chlamydia trachomatis (ct) is the most prevalent bacterial sexually transmitted infection in the world, with more than 100 million cases reported annually. while there have been extensive studies into the adverse effects that ct infection has on the female genital tract, and on the subsequent ability of these women to conceive, studies into the consequences on male fertility have been limited and controversial. this is in part due to the asymptomatic nature of the infection, where it is estimate ...	2014	25386180
sexually transmitted infections in adolescents: maximizing opportunities for optimal care.	sexually transmitted infections are a growing public health concern in canada, with rates of chlamydia trachomatis infection, gonorrhea and syphilis increasing among adolescents and young adults. the present practice point outlines epidemiology, risk factors, laboratory testing and management for c trachomatis, neisseria gonorrhoeae and treponema pallidum, with a lesser focus on hiv. the need for test-of-cure and indications for further investigations are also discussed. the importance of maximi ...	2014	25383001
chlamydia trachomatis protein ct009 is a structural and functional homolog to the key morphogenesis component rodz and interacts with division septal plane localized mreb.	cell division in chlamydiae is poorly understood as apparent homologs to most conserved bacterial cell division proteins are lacking and presence of elongation (rod shape) associated proteins indicate non-canonical mechanisms may be employed. the rod-shape determining protein mreb has been proposed as playing a unique role in chlamydial cell division. in other organisms, mreb is part of an elongation complex that requires rodz for proper function. a recent study reported that the protein encoded ...	2015	25382739
heterogeneity in risk of pelvic inflammatory diseases after chlamydia infection: a population-based study in manitoba, canada.	the association between chlamydia infection and pelvic inflammatory disease (pid) is a key parameter for models evaluating the impact of chlamydia control programs. we quantified this association using a retrospective population-based cohort.	2014	25381374
change in population prevalences of human papillomavirus after initiation of vaccination: the high-throughput hpv monitoring study.	organized human papillomavirus (hpv) vaccination was introduced in sweden in 2012. on-demand vaccination was in effect from 2006 to 2011. we followed the hpv prevalences in southern sweden from 2008 to 2013.	2014	25380734
in silico scrutiny of genes revealing phylogenetic congruence with clinical prevalence or tropism properties of chlamydia trachomatis strains.	microbes possess a multiplicity of virulence factors that confer them the ability to specifically infect distinct biological niches. contrary to what is known for other bacteria, for the obligate intracellular human pathogen chlamydia trachomatis, the knowledge of the molecular basis underlying serovars' tissue specificity is scarce. we examined all ~900 genes to evaluate the association between individual phylogenies and cell-appetence or ecological success of c. trachomatis strains. only ~1% o ...	2014	25378473
identification of linear b-cell epitopes within tarp of chlamydia trachomatis.	chlamydia trachomatis is one of the most prevalent sexually transmitted pathogens. there is currently no commercially available vaccine against c. trachomatis. chlamydial translocated actin-recruiting phosphoprotein (tarp) can induce cellular and humoral immune responses in murine models and has been regarded as a potential vaccine candidate. in this report, the amino acid sequence of tarp was analyzed using computer-assisted techniques to scan b-cell epitopes, and six possible linear b-cell epi ...	2014	25377871
free preconceptual screening examination service in rural areas of hubei province, china in 2012.	this work aims to collect and summarize the outcomes on free preconceptual screening examination in rural areas of hubei province in 2012. moreover, this review promotes further understanding of the status of this activity to provide the family planning commission valid scientific data upon which to construct effective policies.	2014	25375642
the prevalence of chlamydia trachomatis among patients with acute conjunctivitis in kasr alainy ophthalmology clinic.	trachoma is a leading cause of avoidable blindness and endemic conjunctivitis in 57 countries. it infects approximately 84 million people globally, and continues to threaten over 10% of the world's population with the risk of blindness.	2014	25374648
incidence of curable sexually transmissible infections among adolescents and young adults in remote australian aboriginal communities: analysis of longitudinal clinical service data.	to undertake the first comprehensive analysis of the incidence of three curable sexually transmissible infections (stis) within remote australian aboriginal populations and provide a basis for developing new control initiatives.	2015	25371420
ophthalmic infections in children presenting to angkor hospital for children, siem reap, cambodia.	ophthalmic infections cause significant morbidity in cambodian children but aetiologic data are scarce. we investigated the causes of acute eye infections in 54 children presenting to the ophthalmology clinic at angkor hospital for children, siem reap between march and october 2012.	2014	25369774
evaluation of the presence and zoonotic transmission of chlamydia suis in a pig slaughterhouse.	a significant number of studies on pig farms and wild boars worldwide, demonstrate the endemic presence of chlamydia suis in pigs. however, the zoonotic potential of this pathogen, phylogenetically closely related to chlamydia trachomatis, is still uninvestigated. therefore, this study aims to examine the zoonotic transmission in a belgian pig abattoir.	2014	25358497
formulation of the microbicide inp0341 for in vivo protection against a vaginal challenge by chlamydia trachomatis.	the salicylidene acylhydrazide (sa) compounds have exhibited promising microbicidal properties. previous reports have shown the sa compounds, using cell cultures, to exhibit activity against chlamydia trachomatis, herpes simplex virus and hiv-1. in addition, using an animal model of a vaginal infection the sa compound inp0341, when dissolved in a liquid, was able to significantly protect mice from a vaginal infection with c. trachomatis. to expand upon this finding, in this report inp0341 was fo ...	2014	25356686
prevalence of chlamydia trachomatis infection among patients attending infertility and sexually transmitted diseases clinic (std) in kano, north western nigeria.	chlamydia trachomatis is the most common bacterial sexually transmitted disease in the world with severe complications. the aim of this study was to determine the prevalence and possible risk factors of c. trachomatis in kano. there is dearth of information on this subject in this locality.	2014	25352887
prevalence of trichomonas vaginalis by pcr in men attending a primary care urology clinic in south korea.	trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including pcr, few prospective studies present data on male t. vaginalis infection in south korea. in the present study, the prevalence of t. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary ...	2014	25352707
coinfection with chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis: a cross-sectional analysis of positivity and risk factors in remote australian aboriginal communities.	to determine the co-occurrence and epidemiological relationships of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv) in a high-prevalence setting in australia.	2015	25352691
developmental stage oxidoreductive states of chlamydia and infected host cells.	a defining characteristic of chlamydia spp. is their developmental cycle characterized by outer membrane transformations of cysteine bonds among cysteine-rich outer membrane proteins. the reduction-oxidation states of host cell compartments were monitored during the developmental cycle using live fluorescence microscopy. organelle redox states were studied using redox-sensitive green fluorescent protein (rogfp1) expressed in cf15 epithelial cells and targeted to the cytosol, mitochondria, and en ...	2014	25352618
multisite direct determination of the potential for environmental contamination of urine samples used for diagnosis of sexually transmitted infections.	the detection of a sexually transmitted infection (sti) agent in a urine specimen from a young child is regarded as an indicator of sexual contact. false positives may conceivably arise from the transfer of environmental contaminants in clinic toilet or bathroom facilities into urine specimens.	2013	25349693
structural basis of the proteolytic and chaperone activity of chlamydia trachomatis ct441.	chlamydia trachomatis is the most prevalent cause of preventable blindness worldwide and a major reason for infectious infertility in females. several bacterial factors have been implicated in the pathogenesis of c. trachomatis. combining structural and mutational analysis, we have shown that the proteolytic function of ct441 depends on a conserved ser/lys/gln catalytic triad and a functional substrate-binding site within a flexible pdz (postsynaptic density of 95 kda, discs large, and zonula oc ...	2015	25349155
human papillomavirus and chlamydia trachomatis infections in rheumatoid arthritis under anti-tnf therapy: an observational study.	the objective of this study was to evaluate human papillomavirus (hpv) and chlamydia trachomatis (ct) infections in ra patients pre- and post-tnf blocker. fifty female ra patients (acr criteria), who were eligible to anti-tnf therapy [n = 50 at baseline (bl) and n = 45 after 6 months of treatment (6 m)], and 50 age-matched healthy controls were prospectively enrolled. they were assessed for demographic data, gynecologic, sexual, cervical cytology and histological evaluations, disease parameters ...	2015	25348220
chlamydia trachomatis related knowledge and practices in medical practioners--a survey.	chlamydia trachomatis is a frequently encountered condition by general physicians, urologists and infectious diseases specialists. it can affect both genders and causes significant morbidity if not treated properly and promptly. in addition, it can cause ophthalmia neonatorum, which manifests as neonatal conjunctivitis in the newborns.	2014	25345236
drug use, sexual risk behaviour and sexually transmitted infections among swingers: a cross-sectional study in the netherlands.	recreational drug use has been found to be associated with high-risk sexual behaviour and with sexually transmitted infections (sti). this study is the first to assess the prevalence of drug use among swingers (heterosexuals who, as a couple, practise mate swapping or group sex, and/or visit sex clubs for couples), and its association with high-risk sexual behaviour and sti.	2015	25342812