Publications

expression in escherichia coli of chemically synthesized genes for human insulin.	synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ...	1979	85300
alpha fetoprotein levels in amniotic fluid and maternal serum and their use in screening in pregnancy.	during the period january 1975--july 1977 alpha fetoprotein was measured in 263 amniotic fluid samples and 44 maternal serum samples taken in the second trimester of pregnancy. the importance of careful interpretation of results is discussed.	1999	85285
diagnostic value of rectal mucosal acetylcholinesterase levels in hirschsprung's disease.	acetylcholinesterase (ache) activity was measured in rectal biopsy specimens obtained from 68 children aged between 2 days and 14 1/2 years in whom hirschsprung's disease was suspected. the diagnosis was subsequently established in 12; in these, the mean ache activity was found to be 30.5 x 10(-7) units/g tissue (range 16.9 to 63.0). the 56 non-hirschsprung cases had a mean of 5.0 x 10(-7) units/g tissue (s.d. 2.2), the highest value in this group being 10.9. the results were unaffected by age, ...	1999	85002
serum alkaline phosphatase activity in acrodermatitis enteropathica: an index of the serum zinc level.	a significant positive correlation between serum zinc and serum alkaline phosphatase levels was demonstrated in four patients suffering from acrodermatitis enterophathica for which they received oral zinc sulphate therapy. in one of the male patients a significant inverse relation between serum zinc and serum copper was found.	1999	84482
porphyria cutanea tarda associated with lymphoma.	three patients with porphyria cutanea tarda and lymphoma were seen at the mayo clinic. in one patient, the signs and symptoms of porphyria cutanea tarda and lymphoma occurred simultaneously, whereas in the other two, the porphyria was seen before the lymphoma occurred, three years before in one and one and a half years before in the other. involvement of liver or marrow was not related to the development of porphyria cutanea tarda in any of the 3 patients. the porphyria cutanea tarda was not eas ...	1999	84468
electrophoretic changes in serum transferrin and gammaglobulins following rickettsia mooseri infection in cyclophosphamide treated mice.		1978	83756
characterization of the madrid e strain of rickettsia prowazekii purified by renografin density gradient centrifugation.	the avirulent madrid e strain of rickettsia prowazekii cultivated in chicken yolk sacs could be purified successfully with a renografin density gradient method developed previously for rickettsia typhi. recovery during purification, viability, and lack of contamination with host cell components were similar for the two species, although yields of r. prowazekii per yolk sac were lower. purified typhus rickettsiae provided satisfactory antigens in the complement fixation, ouchterlony double-diffus ...	1977	64456
the soluble antigens of rickettsia prowazeki, r. typhi and r. canada. investigation of their interrelationship by various serological methods.	the purpose of this research is the isolation of an eventual species-specific fraction from the "soluble antigen" of rickettsiae. the "soluble antigen" of r. prowazeki (breinl strain), r. typhi (wilmington strain) and r. canada were purified at 25% saturation with ammonium sulphate (psa). corresponding antisera were produced in rabbits. the serological methods used were the complement fixation, the micro-agglutination, the precipitation method in capillary tubes and the immuno-diffusion method c ...	1975	52235
letter: effect of x-ray exposure on nitroblue-tetrazolium test.		1992	48171
seroepidemiological investigations in domestic ruminants from egypt, somalia and jordan for the demonstration of complement fixing antibodies against rickettsia and chlamydia (author's transl).	1450 random serum samples of domestic ruminants from egypt, somalia and jordan were investigated for complement fixing antibodies against rickettsia and chlamydia. between 1.5 and 3.4% of the samples from the animals investigated had antibodies against the rmsf-group of rickettsia, with exception of the sera from somalian cattle and sheep from jordan. antibodies against rickettsia of the typhus-group were found in 4 cattle and 1 goat from jordan and 2 sheep from egypt; by agglutination test with ...	1978	28651
some biological properties of an endotoxic lipopolysaccharide from the typhus group rickettsiae.	a lipophilic thermostable lipopolysaccharide (lps) complex was isolated by phenol extraction from purified suspensions of the typhus group rickettsiae. the lps complex is antigenic and possesses some endotoxic properties such as toxicity for actinomycin d-treated mice, pyrogenicity for rabbits and guinea pigs, ability to elicit hypothermia in white rats and local schwartzman reaction and active cutaneous anaphylaxis in rabbits.	1977	22240