Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| epidemiology of carbapenem-resistant enterobacteriaceae in 7 us communities, 2012-2013. | carbapenem-resistant enterobacteriaceae (cre) are increasingly reported worldwide as a cause of infections with high-mortality rates. assessment of the us epidemiology of cre is needed to inform national prevention efforts. | 2015 | 26436831 |
| accuracy of a new clean-catch technique for diagnosis of urinary tract infection in infants younger than 90 days of age. | to evaluate the accuracy of diagnosing urinary tract infections using a new, recently described, standardized clean-catch collection technique. | 2015 | 26435675 |
| activity of temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam against carbapenem-non-susceptible enterobacteriaceae without carbapenemase production. | treatment options for multidrug-resistant gram-negative infections are scarce and therefore alternatives with a narrow spectrum or new agents are sought. antimicrobial susceptibility to temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam was determined using etest and disk diffusion according to european committee on antimicrobial susceptibility testing (eucast) methodology. a total of 77 carbapenem-nonsusceptible enterobacteriaceae were studied, including klebsiella pneumoniae (26%), ... | 2015 | 26433746 |
| molecular weaponry: diverse effectors delivered by the type vi secretion system. | the type vi secretion system is a widespread bacterial nanomachine, used to deliver toxins directly into eukaryotic or prokaryotic target cells. these secreted toxins, or effectors, act on diverse cellular targets, and their action provides the attacking bacterial cell with a significant fitness advantage, either against rival bacteria or eukaryotic host organisms. in this review, we discuss the delivery of diverse effectors by the type vi secretion system, the modes of action of the so-called ' ... | 2015 | 26432982 |
| factors associated to prevalence and incidence of carbapenem-resistant enterobacteriaceae fecal carriage: a cohort study in a mexican tertiary care hospital. | carbapenem-resistant enterobacteriaceae (cre) infections have emerged as a serious threat to health worldwide. they are associated with increased morbidity and mortality and are capable of silently colonizing the gastrointestinal tract. because of this, there is great interest to characterize the epidemiology of cre carriage and acquisition in healthcare facilities. the aim of this study was to determine the prevalence and factors associated with cre fecal carriage (cre-fc), and risk factors for ... | 2015 | 26431402 |
| incidence, etiology and risk factors associated with neonatal healthcare-associated conjunctivitis: a prospective study from a tertiary care hospital in india. | healthcare-associated conjunctivitis (hac) can lead to serious sequelae including blindness. we conducted a one-year prospective study to determine the epidemiology of neonatal hac at a tertiary-care hospital in india. | 2015 | 26428196 |
| incidence, etiology and risk factors associated with neonatal healthcare-associated conjunctivitis: a prospective study from a tertiary care hospital in india. | healthcare-associated conjunctivitis (hac) can lead to serious sequelae including blindness. we conducted a one-year prospective study to determine the epidemiology of neonatal hac at a tertiary-care hospital in india. | 2015 | 26428196 |
| qsebc, a two-component bacterial adrenergic receptor and global regulator of virulence in enterobacteriaceae and pasteurellaceae. | the qsebc two-component system (tcs) is associated with quorum sensing and functions as a global regulator of virulence. based on sequence similarity within the sensor domain and conservation of an acidic motif essential for signal recognition, qsebc is primarily distributed in the enterobacteriaceae and pasteurellaceae. in escherichia coli, qsec responds to autoinducer-3 and/or epinephrine/norepinephrine. binding of epinephrine/norepinephrine is inhibited by adrenergic antagonists; hence qsec f ... | 2015 | 26426681 |
| qsebc, a two-component bacterial adrenergic receptor and global regulator of virulence in enterobacteriaceae and pasteurellaceae. | the qsebc two-component system (tcs) is associated with quorum sensing and functions as a global regulator of virulence. based on sequence similarity within the sensor domain and conservation of an acidic motif essential for signal recognition, qsebc is primarily distributed in the enterobacteriaceae and pasteurellaceae. in escherichia coli, qsec responds to autoinducer-3 and/or epinephrine/norepinephrine. binding of epinephrine/norepinephrine is inhibited by adrenergic antagonists; hence qsec f ... | 2015 | 26426681 |
| a taqman-based multiplex qpcr assay and dna extraction method for phylotype iib sequevars 1&2 (select agent) strains of ralstonia solanacearum. | ralstonia solanacearum race 3 biovar 2 strains belonging to phylotype iib, sequevars 1 and 2 (iib-1&2) cause brown rot of potato in temperate climates, and are quarantined pathogens in canada and europe. since these strains are not established in the u.s. and because of their potential risk to the potato industry, the u.s. government has listed them as select agents. cultivated geraniums are also a host and have the potential to spread the pathogen through trade, and its extracts strongly inhibi ... | 2015 | 26426354 |
| treatment experience of severe abdominal infection after orthotopic liver transplantation. | this study aims to investigate the causes and treatment experience of severe abdominal infection after orthotopic liver transplantation. clinical data were retrospectively analysed in perioperative severe abdominal infection of 186 orthotopic liver transplantation cases from march 2004 to november 2011. among the 186 patients, 16 cases had severe abdominal infection: five cases had bile duct anastomotic leakage-inducing massive hydrops and infection under liver interstice, 10 cases had extensive ... | 2015 | 26426173 |
| treatment experience of severe abdominal infection after orthotopic liver transplantation. | this study aims to investigate the causes and treatment experience of severe abdominal infection after orthotopic liver transplantation. clinical data were retrospectively analysed in perioperative severe abdominal infection of 186 orthotopic liver transplantation cases from march 2004 to november 2011. among the 186 patients, 16 cases had severe abdominal infection: five cases had bile duct anastomotic leakage-inducing massive hydrops and infection under liver interstice, 10 cases had extensive ... | 2015 | 26426173 |
| heavy metal induced antibiotic resistance in bacterium lsjc7. | co-contamination of antibiotics and heavy metals prevails in the environment, and may play an important role in disseminating bacterial antibiotic resistance, but the selective effects of heavy metals on bacterial antibiotic resistance is largely unclear. to investigate this, the effects of heavy metals on antibiotic resistance were studied in a genome-sequenced bacterium, lsjc7. the results showed that the presence of arsenate, copper, and zinc were implicated in fortifying the resistance of ls ... | 2015 | 26426011 |
| simplified protocol for carba np test for enhanced detection of carbapenemase producers directly from bacterial cultures. | we compared carbapenemase detection among 266 gram-negative bacilli (161 carbapenemase producers) using the carba np tests issued by the clsi (cnpt-clsi) and a novel protocol (cnpt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the clsi tests). the specificities were comparable (100%), but the cnpt-direct was more sensitive (98% versus 84%). the cnpt-direct was easier to perform due to the direct use of colonies and offered ... | 2015 | 26424841 |
| evaluation of the rapidec carba np test for detection of carbapenemases in enterobacteriaceae. | this study evaluated the performance of the rapidec carba np test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing enterobacteriaceae clinical isolates. in total, 252 clinical enterobacteriaceae isolates that had been genetically characterized with respect to carbapenemase, extended-spectrum β-lactamase (esbl), and ampc genes were analyzed; 51/252 isolates (20.2%) were genetically confirmed to be carbapenema ... | 2015 | 26424840 |
| analysis of transmission of mrsa and esbl-e among pigs and farm personnel. | livestock-associated bacteria with resistance to two or more antibiotic drug classes have heightened our awareness for the consequences of antibiotic consumption and spread of resistant bacterial strains in the veterinary field. in this study we assessed the prevalence of concomitant colonization with livestock-associated methicillin-resistant staphylococcus aureus (la-mrsa) and enterobacteriaceae expressing extended-spectrum betalactamases (esbl-e) in farms at the german-dutch border region. na ... | 2015 | 26422606 |
| pathogen profile and drug resistance analysis of spontaneous peritonitis in cirrhotic patients. | to investigate the microbiological characteristics and drug resistance in liver cirrhosis patients with spontaneous peritonitis. | 2015 | 26420967 |
| port type is a possible risk factor for implantable venous access port-related bloodstream infections and no sign of local infection predicts the growth of gram-negative bacilli. | implantable venous access port (ivap)-related blood stream infections (bsis) are one of the most common complications of implantable venous ports. the risk factors and pathogens for ivap-related bsis are still controversial. | 2015 | 26420628 |
| synthesis of novel antimicrobial aryl himachalene derivatives from naturally occurring himachalenes. | five new 2,9,9-trimethyl-6,7,8,9-tetrahydro-benzocyclohepten-5-ylidene-amine derivatives (16a-16e) were synthesized from α-dehydro-ar-himachalene (11) that was originally prepared from an isomeric mixture of α, β and γ himachalenes (10), the abundant sesquiterpenes of cedrus deodara essential oil. in addition, different aryl himachalenes derivatives (9, 12, 14 and 15) were also formed from 11. the structures of the synthesized compounds were confirmed on the basis of their nmr, ir and mass spect ... | 2014 | 26417335 |
| evaluation of the rapidec carba np test kit for detection of carbapenemase-producing gram-negative bacteria. | recently, biomérieux, france, introduced the rapidec carba np test kit for rapid detection of carbapenemase-producing gram-negative bacteria. this kit was evaluated in this study, and we report sensitivity, specificity, and positive and negative predictive values of 92.6%, 96.2%, 95.83%, and 92.6%, respectively. the test was easy to perform and interpret and relatively inexpensive ($5/rs 300 per test) and provides a practical solution for early detection of carbapenemase-producing, multidrug-res ... | 2015 | 26416868 |
| cell wall recycling-linked coregulation of ampc and penb β-lactamases through ampd mutations in burkholderia cenocepacia. | in many gram-negative pathogens, mutations in the key cell wall-recycling enzyme ampd (n-acetyl-anhydromuramyl-l-alanine amidase) affect the activity of the regulator ampr, which leads to the expression of ampc β-lactamase, conferring resistance to expanded-spectrum cephalosporin antibiotics. burkholderia cepacia complex (bcc) species also have these amp homologs; however, the regulatory circuitry and the nature of causal ampd mutations remain to be explored. a total of 92 ampd mutants were obta ... | 2015 | 26416862 |
| efficacy of humanized high-dose meropenem, cefepime, and levofloxacin against enterobacteriaceae isolates producing verona integron-encoded metallo-β-lactamase (vim) in a murine thigh infection model. | we aimed to describe the in vivo activity of humanized pharmacokinetic exposures of meropenem and comparators against verona integron-encoded metallo-β-lactamase (mbl) (vim)-producing enterobacteriaceae in a murine model. levofloxacin activity was predicted by its mic, and cefepime activity displayed variability, whereas meropenem produced a >1 log cfu reduction against all isolates despite high mics indicative of resistance. our results suggest that despite in vitro resistance, high-dose merope ... | 2015 | 26416855 |
| cefepime therapy for monomicrobial enterobacter cloacae bacteremia: unfavorable outcomes in patients infected by cefepime-susceptible dose-dependent isolates. | a new category of cefepime susceptibility, susceptible dose dependent (sdd), for enterobacteriaceae, has been suggested to maximize its clinical use. however, clinical evidence supporting such a therapeutic strategy is limited. a retrospective study of 305 adults with monomicrobial enterobacter cloacae bacteremia at a medical center from 2008 to 2012 was conducted. the patients definitively treated with in vitro active cefepime (cases) were compared with those treated with a carbapenem (controls ... | 2015 | 26416853 |
| molecular characterization of multidrug-resistant klebsiella pneumoniae isolates. | klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. selective pressure, the extensive use of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the emergence of multidrug-resistant (mdr) k. pneumoniae. here, we examined the occurrence, phenotypes and genetic features of mdr k. pneumoniae isolated from patients in intensive care units (icus) at the first affiliated hospital of xiamen ... | 2015 | 26413058 |
| [time series analysis of enterobacter cloacae nosocomial infections in children hospitalized in the pediatric intensive care unit]. | to characterize the time distribution of the incidence of enterobacter cloacae nosocomial infections in children hospitalized in the pediatric intensive care unit (picu) of the first affiliated hospital of xinjiang medical university. | 2015 | 26412176 |
| genetic characterization of carbapenem-resistant enterobacteriaceae and the spread of carbapenem-resistant klebsiella pneumonia st340 at a university hospital in thailand. | carbapenem-resistant enterobacteriaceae (cre) has increasingly spread worldwide in the past decade. the prevalence and characteristics of cre in thailand are unknown. in this study, we conducted a 2-year surveillance of cre among 12,741 clinical isolates of enterobacteriaceae at the largest university hospital in thailand with molecular characterization of beta-lactamase (bla) genes, including carbapenemase genes. the cre prevalence was 1.4%. blakpc-13 and blaimp-14a were the only carbapenemase ... | 2015 | 26407326 |
| ceftolozane/tazobactam. | each month, subscribers to the formulary monograph service receive 5 to 6 well-documented monographs on drugs that are newly released or are in late phase 3 trials. the monographs are targeted to pharmacy & therapeutics committees. subscribers also receive monthly 1-page summary monographs on agents that are useful for agendas and pharmacy/nursing in-services. a comprehensive target drug utilization evaluation/medication use evaluation (due/mue) is also provided each month. with a subscription, ... | 2015 | 26405345 |
| yersinia ruckeri, the causative agent of enteric redmouth disease in fish. | enteric redmouth disease (erm) is a serious septicemic bacterial disease of salmonid fish species. it is caused by yersinia ruckeri, a gram-negative rod-shaped enterobacterium. it has a wide host range, broad geographical distribution, and causes significant economic losses in the fish aquaculture industry. the disease gets its name from the subcutaneous hemorrhages, it can cause at the corners of the mouth and in gums and tongue. other clinical signs include exophthalmia, darkening of the skin, ... | 2015 | 26404907 |
| association of necrotizing wounds colonized by maggots with ignatzschineria-associated septicemia. | 2015 | 26402740 | |
| comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients. | the genetic disorder cystic fibrosis is a life-limiting condition affecting ∼70,000 people worldwide. targeted, early, treatment of the dominant infecting species, pseudomonas aeruginosa, has improved patient outcomes; however, there is concern that other species are now stepping in to take its place. in addition, the necessarily long-term antibiotic therapy received by these patients may be providing a suitable environment for the emergence of antibiotic resistance. to investigate these issues, ... | 2015 | 26401445 |
| [shoulder tuberculosis masked by concomitant enterobacter cloacae infection: report of a case]. | 2015 | 26401203 | |
| oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions. | the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ... | 2015 | 26399712 |
| could essential oils of green and black pepper be used as food preservatives? | black and green pepper essential oils were used in this study in order to determine the chemical composition, in vitro antimicrobial activity against food spoilage microorganisms and in situ oils effect on food microorganism, after incorporation in chicken soup, by suggested methodology for calculation of growth inhibition concentrations (gic50). chemical analysis revealed a total of 34 components. the major constituent of black pepper oil was trans-caryophyllene (30.33 %), followed by limonene ... | 2015 | 26396402 |
| radiations and biodegradation techniques for detoxifying carica papaya seed oil for effective dietary and industrial use. | benzyl isothiocyanate (bitc) is toxic in high concentration. the capacity of aspergillus niger, microwave and ultraviolet radiations to reduce the bitc levels in carica papaya linn seed oil were assessed in vitro. bitc at different concentrations were periodically exposed to microwave and ultraviolet radiations for 30 min and 10 h, respectively; and to identify aspergillus niger for 4 days. microwave radiation significantly reduced (p < 0.05) bitc levels (0.0272, 0.0544, and 0.0816 μmol) to 12.1 ... | 2015 | 26396392 |
| modulation of gut microbiota by berberine and metformin during the treatment of high-fat diet-induced obesity in rats. | accumulating evidence suggests that the gut microbiota is an important factor in mediating the development of obesity-related metabolic disorders, including type 2 diabetes. metformin and berberine, two clinically effective drugs for treating diabetes, have recently been shown to exert their actions through modulating the gut microbiota. in this study, we demonstrated that metformin and berberine similarly shifted the overall structure of the gut microbiota in rats. both drugs showed reverting e ... | 2015 | 26396057 |
| characteristics of korean patients with antithyroid drug-induced agranulocytosis: a multicenter study in korea. | antithyroid drugs (atds) can lead to the development of agranulocytosis, which is the most serious adverse effect. characteristics of atd-induced agranulocytosis (aia) have seldom been reported due to the rarity. in this study, we characterized the clinical features for aia in korean patients. | 2015 | 26394729 |
| coding of electronic laboratory reports for biosurveillance, selected united states hospitals, 2011. | objective electronic laboratory reporting has been promoted as a public health priority. the office of the u.s. national coordinator for health information technology has endorsed two coding systems: logical observation identifiers names and codes (loinc) for laboratory test orders and systemized nomenclature of medicine-clinical terms (snomed ct) for test results. materials and methods we examined loinc and snomed ct code use in electronic laboratory data reported in 2011 by 63 non-federal hosp ... | 2015 | 26392850 |
| characterization of tn3000, a transposon responsible for blandm-1 dissemination among enterobacteriaceae in brazil, nepal, morocco, and india. | in enterobacteriaceae, the blandm genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. in august 2013, we identified ndm-1-producing escherichia coli and enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in rio de janeiro, brazil, who had no history of travel abroad. complete dna sequencing using the illumina platform and annotation of the two plasmids harboring the bland ... | 2015 | 26392506 |
| genetic contexts of blandm-1 in patients carrying multiple ndm-producing strains. | the carbapenem resistance determinant blandm-1 has been found in various gram-negative bacteria and upon different plasmid replicon types (inc). here, we present four patients within two hospitals in pakistan harboring between two and four ndm-1-producing gram-negative bacilli of different species coresident in their stool samples. we characterize the blandm-1 genetic contexts of these 11 ndm-1-producing gram-negative bacilli in addition to other antimicrobial resistance mechanisms, plasmid repl ... | 2015 | 26392493 |
| in vitro activities of ceftazidime-avibactam, aztreonam-avibactam, and a panel of older and contemporary antimicrobial agents against carbapenemase-producing gram-negative bacilli. | among 177 carbapenemase-producing gram-negative bacilli (108 kpc, 32 ndm, 11 imp, 8 oxa-48, 4 oxa-181, 2 oxa-232, 5 imi, 4 vim, and 3 sme producers), aztreonam-avibactam was active against all isolates except two ndm producers with elevated mics of 8/4 and 16/4 mg/liter; ceftazidime-avibactam was active against all kpc-, imi-, sme-, and most oxa-48 group-producing isolates (93%) but not metallo-β-lactamase producers. among older and contemporary antimicrobials, the most active were colistin, tig ... | 2015 | 26392487 |
| genetic and biochemical characterization of fri-1, a carbapenem-hydrolyzing class a β-lactamase from enterobacter cloacae. | an enterobacter cloacae isolate was recovered from a rectal swab from a patient hospitalized in france with previous travel to switzerland. it was resistant to penicillins, narrow- and broad-spectrum cephalosporins, aztreonam, and carbapenems but remained susceptible to expanded-spectrum cephalosporins. whereas pcr-based identification of the most common carbapenemase genes failed, the biochemical carba np test ii identified an ambler class a carbapenemase. cloning experiments followed by sequen ... | 2015 | 26392482 |
| [bacterial infections in the early period after allogeneic bone marrow transplantation]. | to study the incidence and risk factors of bacterial infections and the efficiency of empirical antibacterial therapy in patients in the early period after allogeneic hematopoietic stem cell transplantation (allo-hsct). | 2015 | 26390730 |
| contact-dependent growth inhibition (cdi) and cdib/cdia two-partner secretion proteins. | bacteria have developed several strategies to communicate and compete with one another in complex environments. one important mechanism of inter-bacterial competition is contact-dependent growth inhibition (cdi), in which gram-negative bacteria use cdib/cdia two-partner secretion proteins to suppress the growth of neighboring target cells. cdib is an omp85 outer-membrane protein that exports and assembles cdia exoproteins onto the inhibitor cell surface. cdia binds to receptors on susceptible ba ... | 2015 | 26388411 |
| application of metagenomic sequencing to food safety: detection of shiga toxin-producing escherichia coli on fresh bagged spinach. | culture-independent diagnostics reduce the reliance on traditional (and slower) culture-based methodologies. here we capitalize on advances in next-generation sequencing (ngs) to apply this approach to food pathogen detection utilizing ngs as an analytical tool. in this study, spiking spinach with shiga toxin-producing escherichia coli (stec) following an established fda culture-based protocol was used in conjunction with shotgun metagenomic sequencing to determine the limits of detection, sensi ... | 2015 | 26386062 |
| conformational toggling controls target site choice for the heteromeric transposase element tn7. | the bacterial transposon tn7 facilitates horizontal transfer by directing transposition into actively replicating dna with the element-encoded protein tnse. structural analysis of the c-terminal domain of wild-type tnse identified a novel protein fold including a central v-shaped loop that toggles between two distinct conformations. the structure of a robust tnse gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates tnse acti ... | 2015 | 26384427 |
| complete sequence of a blaoxa-48-harboring incl plasmid from an enterobacter cloacae clinical isolate. | we report a 63,584-bp conjugative incl plasmid (pur17313-1) from an enterobacter cloacae clinical isolate, containing a blaoxa-48 gene. the plasmid sequence also carried important mobile genetic elements involved in the spread of antibiotic resistance, namely, the tn1999.2 composite transposon, which enclosed blaoxa-48-, integrase-, and transposase-encoding genes. | 2015 | 26383652 |
| risk factors of infectious complications following flexible ureteroscope with a holmium laser: a retrospective study. | we aimed to evaluate the effectiveness of flexible ureteroscope for treating kidney stones and the risk factors for infectious complications following flexible ureteroscope (furs) with a holmium laser. | 2015 | 26379932 |
| metabolic engineering of escherichia coli for production of (2s,3s)-butane-2,3-diol from glucose. | butane-2,3-diol (2,3-bd) is a fuel and platform biochemical with various industrial applications. 2,3-bd exists in three stereoisomeric forms: (2r,3r)-2,3-bd, meso-2,3-bd and (2s,3s)-2,3-bd. microbial fermentative processes have been reported for (2r,3r)-2,3-bd and meso-2,3-bd production. | 2015 | 26379775 |
| analysis of the pathogenic potential of nosocomial pseudomonas putida strains. | pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. in this study we describe the multilocus sequence typing of four p. putida strains (hb13667, hb8234, hb4184, and hb3267) isolated from in-patients at the besançon hospital (france). the four isolates (in particular hb3267) were resistant to a number of antibiotics. the pathogenicity and virulence potential of the strains was tested ex ... | 2015 | 26379646 |
| overproduction of the membrane-bound [nife]-hydrogenase in thermococcus kodakarensis and its effect on hydrogen production. | the hyperthermophilic archaeon thermococcus kodakarensis can utilize sugars or pyruvate for growth. in the absence of elemental sulfur, the electrons via oxidation of these substrates are accepted by protons, generating molecular hydrogen (h2). the hydrogenase responsible for this reaction is a membrane-bound [nife]-hydrogenase (mbh). in this study, we have examined several possibilities to increase the protein levels of mbh in t. kodakarensis by genetic engineering. highest levels of intracellu ... | 2015 | 26379632 |
| antibacterial effects of cinnamon: from farm to food, cosmetic and pharmaceutical industries. | herbs and spices have been used since ancient times, because of their antimicrobial properties increasing the safety and shelf life of food products by acting against foodborne pathogens and spoilage bacteria. plants have historically been used in traditional medicine as sources of natural antimicrobial substances for the treatment of infectious disease. therefore, much attention has been paid to medicinal plants as a source of alternative antimicrobial strategies. moreover, due to the growing d ... | 2015 | 26378575 |
| detection of polyclonality among clinical isolates from prosthetic joint infections. | prosthetic joint infection (pji) is an increasingly important health concern in the western world due to the rising number of joint arthroplasties. although most infections are considered to be monomicrobial, the introduction of sonication procedures has led to an increase in the detection of polymicrobial infections. to date, no published studies have investigated the presence of different clones of the same species in the infected patient. the objective of this study was to analyze whether the ... | 2015 | 26378278 |
| premature baby with extreme hyponatraemia (95 mmol per litre): a case report. | whilst mild neonatal hyponatraemia is common and relatively harmless, extreme hyponatraemia of 95 mmol per litre has never been reported in a premature baby and such a level could be associated with immediate as well as long-lasting detrimental effects on health. | 2015 | 26377549 |
| loss of membrane-bound lytic transglycosylases increases outer membrane permeability and β-lactam sensitivity in pseudomonas aeruginosa. | the opportunistic pathogen pseudomonas aeruginosa is a leading cause of nosocomial infections. its relatively impermeable outer membrane (om) limits antibiotic entry, and a chromosomally encoded ampc β-lactamase inactivates β-lactam antibiotics. ampc expression is linked to peptidoglycan (pg) recycling, and soluble (slt) or membrane-bound (mlt) lytic transglycosylases are responsible for generating the anhydromuropeptides that induce ampc expression. thus, inhibition of lt activity could reduce ... | 2015 | 26374494 |
| recent advances in engineering topography mediated antibacterial surfaces. | the tendency of bacterial cells to adhere and colonize a material surface leading to biofilm formation is a fundamental challenge underlying many different applications including microbial infections associated with biomedical devices and products. although, bacterial attachment to surfaces has been extensively studied in the past, the effect of surface topography on bacteria-material interactions has received little attention until more recently. we review the recent progress in surface topogra ... | 2015 | 26372264 |
| antibiotic-impregnated cement spacer as definitive management for osteomyelitis. | osteomyelitis is a challenge for orthopaedic surgeons. there is a lack of scientific evidence to guide treatment. the purpose of this study was to report the clinical outcome of unplanned retention of antibiotic-impregnated cement spacer (acs) in the management of osteomyelitis. | 2015 | 26370895 |
| purpura fulminans due to enterobacter cloacae. | 2015 | 26370876 | |
| construction of recombinant pichia pastoris carrying a constitutive avbd9 gene and analysis of its activity. | avian beta-defensin 9 (avbd9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, avbd9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. using the preferred codon of pichia pastoris, the mature avbd9 peptide was designed and synthesized, based on th ... | 2015 | 26370794 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| in vitro activities of novel antimicrobial combinations against extensively drug-resistant acinetobacter baumannii. | extensively drug-resistant (xdr) acinetobacter spp. have emerged as a cause of nosocomial infections, especially under conditions of intensive care. unfortunately, resistance to colistin is increasing and there is a need for new therapeutic options. we aimed to study the effect of some novel combinations against xdr acinetobacter baumannii in an in vitro pharmacokinetics-pharmacodynamics (pk/pd) model. three nonrelated clinical strains of xdr a. baumannii were investigated. antibiotic-simulated ... | 2015 | 26369956 |
| characterization of the bacterioferritin/bacterioferritin associated ferredoxin protein-protein interaction in solution and determination of binding energy hot spots. | mobilization of iron stored in the interior cavity of bfrb requires electron transfer from the [2fe−2s] cluster in bfd to the core iron in bfrb. a crystal structure of the pseudomonas aeruginosa bfrb:bfd complex revealed that bfrb can bind up to 12 bfd molecules at 12 structurally identical binding sites, placing the [2fe−2s] cluster of each bfd immediately above a heme group in bfrb [yao, h., et al. (2012) j. am. chem. soc., 134, 13470−13481]. we report here study aimed at characterizing the st ... | 2015 | 26368531 |
| clinical usefulness of procalcitonin and c-reactive protein as outcome predictors in critically ill patients with severe sepsis and septic shock. | sepsis is a major cause of mortality and morbidity in critically ill patients. procalcitonin (pct) and c-reactive protein (crp) are the most frequently used biomarkers in sepsis. we investigated changes in pct and crp concentrations in critically ill patients with sepsis to determine which biochemical marker better predicts outcome. we retrospectively analyzed 171 episodes in 157 patients with severe sepsis and septic shock who were admitted to the samsung medical center intensive care unit from ... | 2015 | 26367532 |
| considerations about antimicrobial stewardship in settings with epidemic extended-spectrum β-lactamase-producing or carbapenem-resistant enterobacteriaceae. | infections caused by gram-negative bacteria (gnb) resistant to multiple classes of antibiotics are increasing in many hospitals. extended-spectrum β-lactamase (esbl)-producing and carbapenem-resistant enterobacteriaceae in particular are now endemic in many parts of the world and represent a serious public health threat. in this era, antimicrobial stewardship programs are essential as targeted and responsible use of antibiotics improves patient outcomes and hopefully limits the selective pressur ... | 2015 | 26362292 |
| genetic environment of plasmid mediated ctx-m-15 extended spectrum beta-lactamases from clinical and food borne bacteria in north-eastern india. | the study investigated the presence of ctx-m-15 type extended spectrum beta-lactamases (esbl), compared their genetic arrangements and plasmid types in gram negative isolates of hospital and food origin in north-east india. from september 2013 to april 2014, a total of 252 consecutive, non-duplicate clinical isolates and 88 gram negative food isolates were selected. phenotypic and molecular characterization of esbl genes was performed. presence of integrons and gene cassettes were analyzed by in ... | 2015 | 26361395 |
| the initial state of the human gut microbiome determines its reshaping by antibiotics. | microbiome studies have demonstrated the high inter-individual diversity of the gut microbiota. however, how the initial composition of the microbiome affects the impact of antibiotics on microbial communities is relatively unexplored. to specifically address this question, we administered a second-generation cephalosporin, cefprozil, to healthy volunteers. stool samples gathered before antibiotic exposure, at the end of the treatment and 3 months later were analysed using shotgun metagenomic se ... | 2015 | 26359913 |
| the initial state of the human gut microbiome determines its reshaping by antibiotics. | microbiome studies have demonstrated the high inter-individual diversity of the gut microbiota. however, how the initial composition of the microbiome affects the impact of antibiotics on microbial communities is relatively unexplored. to specifically address this question, we administered a second-generation cephalosporin, cefprozil, to healthy volunteers. stool samples gathered before antibiotic exposure, at the end of the treatment and 3 months later were analysed using shotgun metagenomic se ... | 2015 | 26359913 |
| brown algae (phaeophyceae) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products. | eight species of brown algae (phaeophyceae) from the coast of madagascar have been investigated for their chemical constituents. fucosterol (3) was obtained as the most abundant compound. the brown alga sargassum ilicifolium was the source for the first isolation of the terpenoid c27-alcohol 1,1',2-trinorsqualenol (1) from marine sources. from s. incisifolium we isolated the highly unsaturated glycolipid 1-o-palmitoyl-2-o-stearidonoyl-3-o-β-d-galactopyranosylglycerol (4) and we report the first ... | 2015 | 26358714 |
| infectious diseases linked to cross-contamination of flexible endoscopes. | flexible endoscopes are widely used to examine, diagnose, and treat medical disorders. while the risk of endoscopy-related transmission of infection is estimated to be very low, more health care-associated infections are related to contaminated endoscopes than to any other medical device. flexible endoscopes can get highly contaminated with microorganisms, secretions and blood during use. the narrow lumens and multiple internal channels make the cleaning of flexible endoscopes a complex and diff ... | 2015 | 26355428 |
| transcriptional analysis of the conjugal transfer genes of rickettsia bellii rml 369-c. | rickettsia bellii is an obligate intracellular bacterium that is one of the few rickettsiae that encode a complete set of conjugative transfer (tra) genes involved in bacterial conjugation and has been shown to exhibit pili-like structures. the reductive genomes of rickettsiae beg the question whether the tra genes are nonfunctional or functioning to enhance the genetic plasticity and biology of rickettsiae. we characterized the transcriptional dynamics of r. bellii tra genes in comparison to ge ... | 2015 | 26352829 |
| the antibacterial activity of acetic acid against biofilm-producing pathogens of relevance to burns patients. | localised infections, and burn wound sepsis are key concerns in the treatment of burns patients, and prevention of colonisation largely relies on biocides. acetic acid has been shown to have good antibacterial activity against various planktonic organisms, however data is limited on efficacy, and few studies have been performed on biofilms. | 2015 | 26352256 |
| adhesins involved in attachment to abiotic surfaces by gram-negative bacteria. | during the first step of biofilm formation, initial attachment is dictated by physicochemical and electrostatic interactions between the surface and the bacterial envelope. depending on the nature of these interactions, attachment can be transient or permanent. to achieve irreversible attachment, bacterial cells have developed a series of surface adhesins promoting specific or nonspecific adhesion under various environmental conditions. this article reviews the recent advances in our understandi ... | 2015 | 26350310 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between july 1982 and march 2012]. | this is an integrated summary of the results obtained from a 3-decade multicenter study on bacteria isolated from surgical infections in japan between july 1982 and march 2012. during the 3-decade study, 11,196 strains were isolated from 4,787 patients consisting of 2,132 patients with primary infection and 2,655 patients with surgical site infection. almost half of the primary infection was peritonitis, which accounted for 42.3%-55.5%. in contrast, most of the surgical site infection was wound ... | 2015 | 26349116 |
| necrotizing fasciitis secondary to a primary suture for anoperineal trauma by motorcycle accident in a healthy adult. | a 41-year-old man experienced a swollen scrotum three days after a motorcycle accident and presented to our hospital. he had had a primary suture repair for anoperineal trauma in an outside hospital at the time of the injury. he presented to us with general fatigue, low grade fevers, and perineal pain. abdominal computed tomography showed subcutaneous emphysema from the scrotum to the left chest. the sutured wound had foul-smelling discharge and white exudate. we made the diagnosis of necrotizin ... | 2015 | 26347831 |
| comparative genomics analysis of pkf3-94 in klebsiella pneumoniae reveals plasmid compatibility and horizontal gene transfer. | in order to get insights into plasmid evolution and the dissemination of multidrug resistance, we performed extensive comparative genomics analyses of the klebsiella pneumoniae plasmid pkf3-94 and some of its related plasmids. pkf3-94 is one of three plasmids isolated from the k. pneumoniae strain kf3. of the 144 putative genes it harbors, 69 can be functionally assigned to be involved in transfer conjugation, transfer leading, antimicrobial resistance, transposon function, and plasmid replicati ... | 2015 | 26347723 |
| regulation of indole-3-acetic acid biosynthesis by branched-chain amino acids in enterobacter cloacae uw5. | the soil bacterium enterobacter cloacae uw5 produces the rhizosphere signaling molecule indole-3-acetic acid (iaa) via the indolepyruvate pathway. expression of indolepyruvate decarboxylase, a key pathway enzyme encoded by ipdc, is upregulated by the transcription factor tyrr in response to aromatic amino acids. some members of the tyrr regulon may also be controlled by branched-chain amino acids and here we show that expression from the ipdc promoter and production of iaa are downregulated by v ... | 2015 | 26347301 |
| a novel microbisporicin producer identified by early dereplication during lantibiotic screening. | with the increasing need of effective antibiotics against multi-drug resistant pathogens, lantibiotics are an attractive option of a new class of molecules. they are ribosomally synthetized and posttranslationally modified peptides possessing potent antimicrobial activity against aerobic and anaerobic gram-positive pathogens, including those increasingly resistant to β-lactams and glycopeptides. some of them (actagardine, mersacidin, planosporicin, and microbisporicin) inhibit cell wall biosynth ... | 2015 | 26346738 |
| impact of the new delhi metallo-beta-lactamase on beta-lactam antibiotics. | since the first new delhi metallo-beta-lactamase (ndm) report in 2009, ndm has spread globally causing various types of infections. ndm-positive organisms produce in vitro resistance phenotypes to carbapenems and many other antimicrobials. it is thus surprising that the literature examining clinical experiences with ndm does not report corresponding poor clinical outcomes. there are many instances where good clinical outcomes are described, despite a mismatch between administered antimicrobials ... | 2015 | 26345624 |
| synthesis of silver nanoparticles: chemical, physical and biological methods. | silver nanoparticles (nps) have been the subjects of researchers because of their unique properties (e.g., size and shape depending optical, antimicrobial, and electrical properties). a variety of preparation techniques have been reported for the synthesis of silver nps; notable examples include, laser ablation, gamma irradiation, electron irradiation, chemical reduction, photochemical methods, microwave processing, and biological synthetic methods. this review presents an overview of silver nan ... | 2014 | 26339255 |
| clinical performance of check-direct cpe, a multiplex pcr for direct detection of bla(kpc), bla(ndm) and/or bla(vim), and bla(oxa)-48 from perirectal swabs. | we evaluated the clinical performance of check-direct cpe for carbapenemase detection directly from 301 perirectal swabs (258 patients) in a nonoutbreak setting. culture of a pcr-confirmed, carbapenemase-containing organism, or history of colonization with such organism within the previous 2 weeks, was used as the reference standard. check-direct cpe demonstrated a sensitivity value, specificity value, positive predictive value (ppv), and negative predictive value (npv) of 100% (all bla(kpc)), 8 ... | 2015 | 26338860 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial bacillus amyloliquefaciens sqr9. | the plant growth-promoting rhizobacteria (pgpr) strain bacillus amyloliquefaciens sqr9, isolated from the cucumber rhizosphere, protects the host plant from pathogen invasion and promotes plant growth through efficient root colonization. the phytohormone indole-3-acetic acid (iaa) has been suggested to contribute to the plant-growth-promoting effect of bacillus strains. the possible iaa synthetic pathways in b. amyloliquefaciens sqr9 were investigated in this study, using a combination of chemic ... | 2015 | 26337367 |
| atopic allergy and chronic inflammation of the oral mucosa in a 3-year-old boy after heart transplantation - diagnostic and therapeutic difficulties. | in recent years, we have been observing an increased proportion of atopic diseases in children after solid organ transplantation. the pathogenesis of post-transplantation allergy is not completely understood and probably involves several factors, including immunosuppressive therapy. in this paper we present a case of 3-year old boy, after orthotopic heart transplantation at 6 months of age, with symptoms of food allergy associated with atopic dermatitis and changes in the orofacial area. the men ... | 2015 | 26336506 |
| gentamicin-impregnated collagen sponge for preventing sternal wound infection after cardiac surgery. | the frequency of sternal wound infection (swi) after cardiac surgery ranges from 0.5% to 8% and is associated with significant morbidity, mortality, and treatment cost. perioperative antibiotic prophylaxis is not sufficient to fully prevent the contamination of the surgical access site. one of the most effective methods for the prevention of wound infection seems to be the use of gentamicin-impregnated collagen sponge, which is successfully used in abdominal and orthopedic surgery. surgically im ... | 2014 | 26336388 |
| carbapenem-resistant and carbapenem-susceptible isogenic isolates of klebsiella pneumoniae st101 causing infection in a tertiary hospital. | in this study we describe the clinical and molecular characteristics of an outbreak due to carbapenem-resistant klebsiella pneumoniae (cr-kp) producing ctx-m-15 and oxa-48 carbapenemase. isogenic strains, carbapenem-susceptible k. pneumoniae (cs-kp) producing ctx-m-15, were also involved in the outbreak. | 2015 | 26335352 |
| high frequency of polymicrobial infections after surgical resection of malignant bone and soft tissue tumors: a retrospective cohort study. | surgical resection of a malignant bone tumor (bt) or soft tissue tumor (stt), with or without prosthetic replacement, carries a high risk of developing postoperative infections. there is limited knowledge on the bacteriological spectrum of these postsurgical infections that necessitate empirical antibiotic therapy. the aim of this study was to analyze the incidence and microbiological features of site infections following bt or stt resection. | 2015 | 26334238 |
| suffer the infants: a severe case of pertussis in oregon, 2012. | pertussis remains a public health concern in oregon, especially among young infants. the disease can be severe in this age group and is associated with a high inpatient cost. this report describes an oregon infant who was hospitalized with pertussis for 90 days, required extracorporeal oxygenation for 43 days, suffered complications including stroke, and had hospital charges totaling $1.5 million. pertussis morbidity among young infants argues for vaccination of women during each pregnancy and o ... | 2015 | 26327720 |
| correction: a five-year experience of carbapenem resistance in enterobacteriaceae causing neonatal septicaemia: predominance of ndm-1. | 2015 | 26327517 | |
| extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) infections: are carbapenem alternatives achievable in daily practice? | to avoid the use of carbapenems, alternatives such as cephamycin, piperacillin-tazobactam, and others are suggested for the treatment of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) infections. the aim of this study was to evaluate the frequency and the feasibility of antimicrobial de-escalation for esbl-pe-related infections. | 2015 | 26327124 |
| vancomycin-associated nephrotoxicity: the obesity factor. | current recommendations suggest that vancomycin dosing utilize actual rather than ideal body weight in obese patients. thus, obese patients may be at greater risk for nephrotoxicity. the purpose of this study was to compare the incidence of nephrotoxicity in vancomycin-treated obese and lean patients at our institution, where unadjusted, actual body weight-based dosing (capped at 2 g per dose twice daily) is used. we expected obese patients to experience a greater incidence of nephrotoxicity tha ... | 2015 | 26324996 |
| clinical outcomes associated with polymyxin b dose in patients with bloodstream infections due to carbapenem-resistant gram-negative rods. | there is significant variation in the use of polymyxin b (pmb), and optimal dosing has not been defined. the purpose of this retrospective study was to evaluate the relationship between pmb dose and clinical outcomes. we included patients with bloodstream infections (bsis) due to carbapenem-resistant gram-negative rods who received ≥48 h of intravenous pmb. the objective was to evaluate the association between pmb dose and 30-day mortality, clinical cure at day 7, and development of acute kidney ... | 2015 | 26324272 |
| the effectiveness of local antibiotics in treating chronic osteomyelitis in a cohort of 50 patients with an average of 4 years follow-up. | the treatment of chronic osteomyelitis requires both appropriate surgical and antibiotic management. prolonged intravenous antibiotic therapy followed by oral therapy is widely utilised. despite this, the long-term recurrence rate can be up to 30%. a cohort of 50 patients from a 7-year period, 2003 to 2010, with chronic osteomyelitis was identified. this cohort was treated by surgical marginal resection in combination with local application of antibiotics (collatamp g - gentamicin in a collagen ... | 2015 | 26322143 |
| innate and adaptive humoral responses coat distinct commensal bacteria with immunoglobulin a. | immunoglobulin a (iga) is prominently secreted at mucosal surfaces and coats a fraction of the intestinal microbiota. however, the commensal bacteria bound by iga are poorly characterized and the type of humoral immunity they elicit remains elusive. we used bacterial flow cytometry coupled with 16s rrna gene sequencing (iga-seq) in murine models of immunodeficiency to identify iga-bound bacteria and elucidate mechanisms of commensal iga targeting. we found that residence in the small intestine, ... | 2015 | 26320660 |
| draft genome sequences of two imp-4-producing escherichia coli sequence type 131 isolates in australia. | we report the draft genome sequences of two unrelated cases of escherichia coli sequence type 131 (st131) possessing the carbapenemase gene blaimp-4. the e. coli st131 sn5 isolate also possessed blashv-12 and plasmid-mediated quinolone-resistance genes. wider dissemination of blaimp-4 may occur due to the blaimp-4-carrying l/m or hi2 plasmids among e. coli st131 isolates. | 2015 | 26316639 |
| candida albicans inhibits pseudomonas aeruginosa virulence through suppression of pyochelin and pyoverdine biosynthesis. | bacterial-fungal interactions have important physiologic and medical ramifications, but the mechanisms of these interactions are poorly understood. the gut is host to trillions of microorganisms, and bacterial-fungal interactions are likely to be important. using a neutropenic mouse model of microbial gastrointestinal colonization and dissemination, we show that the fungus candida albicans inhibits the virulence of the bacterium pseudomonas aeruginosa by inhibiting p. aeruginosa pyochelin and py ... | 2015 | 26313907 |
| multiple infectious complications in a severely injured patient with single nucleotide polymorphisms in important innate immune response genes. | trauma is a major public health problem worldwide. infectious complications, sepsis, and multiple organ dysfunction syndrome (mods) remain important causes for morbidity and mortality in patients who survive the initial trauma. there is increasing evidence for the role of genetic variation in the innate immune system on infectious complications in severe trauma patients. we describe a trauma patient with multiple infectious complications caused by multiple micro-organisms leading to prolonged ho ... | 2015 | 26312121 |
| [analysis of clinical outcome and impact factors of twin-to-twin transfusion syndrome with anterior placenta treated by fetoscopic laser photocoagulation]. | to analyze the clinical outcome and impact factors of twin-to-twin transfusion syndrome (ttts) with anterior placenta treated by fetoscopic selective laser coagulation of placental vessels (slcpv). | 2015 | 26311450 |
| dissemination of multiresistant enterobacter cloacae isolates producing oxa-48 and ctx-m-15 in a spanish hospital. | twenty-one multiresistant enterobacter cloacae isolates producing oxa-48 (n=10), ctx-m-15 (n=7) or both (n=4) β-lactamases were detected in a spanish hospital during a 1-year period (june 2013 to june 2014). the isolates were also resistant to non-β-lactam antimicrobials, further complicating the therapeutic options. genotyping of the isolates identified two major clones (st74 and st66) that caused prolonged outbreaks in different buildings of the hospital as well as some sporadic isolates (st78 ... | 2015 | 26307466 |
| pneumonia among adults hospitalized with laboratory-confirmed seasonal influenza virus infection-united states, 2005-2008. | influenza and pneumonia combined are the leading causes of death due to infectious diseases in the united states. we describe factors associated with pneumonia among adults hospitalized with influenza. | 2015 | 26307108 |