Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| genome sequence of the quorum-quenching agrobacterium tumefaciens strain wrt31. | agrobacterium tumefaciens strain wrt31 is a quorum-sensing signal-degrading bacterium that has been isolated from the rhizosphere of tobacco plants. this strain belongs to a. tumefaciens genomovar g1, is avirulent on various putative host plants, devoid of ti plasmid, and contains the blcc gene encoding a gamma-butyrolactonase. | 2013 | 23969055 |
| genetic circuits that govern bisexual and unisexual reproduction in cryptococcus neoformans. | cryptococcus neoformans is a human fungal pathogen with a defined sexual cycle. nutrient-limiting conditions and pheromones induce a dimorphic transition from unicellular yeast to multicellular hyphae and the production of infectious spores. sexual reproduction involves cells of either opposite (bisexual) or one (unisexual) mating type. bisexual and unisexual reproduction are governed by shared components of the conserved pheromone-sensing cpk1 mapk signal transduction cascade and by mat2, the m ... | 2013 | 23966871 |
| biological importance of marine algae. | marine organisms are potentially prolific sources of highly bioactive secondary metabolites that might represent useful leads in the development of new pharmaceutical agents. algae can be classified into two main groups; first one is the microalgae, which includes blue green algae, dinoflagellates, bacillariophyta (diatoms)… etc., and second one is macroalgae (seaweeds) which includes green, brown and red algae. the microalgae phyla have been recognized to provide chemical and pharmacological no ... | 2009 | 23960716 |
| optimization, validation, and application of a real-time pcr protocol for quantification of viable bacterial cells in municipal sewage sludge and biosolids using reporter genes and escherichia coli. | biosolids result from treatment of sewage sludge to meet jurisdictional standards, including pathogen reduction. once government regulations are met, materials can be applied to agricultural lands. culture-based methods are used to enumerate pathogen indicator microorganisms but may underestimate cell densities, which is partly due to bacteria existing in a viable but non-culturable physiological state. viable indicators can also be quantified by realtime polymerase chain reaction (qpcr) used wi ... | 2013 | 23958912 |
| inducible expression of p50 from tmv for increased resistance to bacterial crown gall disease in tobacco. | the dominant tobacco mosaic virus (tmv) resistance gene n induces a hypersensitive response upon tmv infection and protects tobacco against systemic spread of the virus. it has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence genes. to reprogramme the resistance response of n towards bacterial pathogens, the helicase domain (p50) of the tmv replicase, the avirulence gene of n, was linked to synthetic prom ... | 2014 | 23955710 |
| response of chickpea genotypes to agrobacterium-mediated delivery of chickpea chlorotic dwarf virus (cpcdv) genome and identification of resistance source. | chickpea stunt disease caused by chickpea chlorotic dwarf virus (cpcdv) (genus mastrevirus, family geminiviridae) is the most important biotic stress affecting chickpea crops worldwide. a survey conducted on the incidence of stunt disease clearly revealed high incidence of the disease with severe symptom expression in both indigenous and imported genotypes. to manage the disease in a sustainable way, resistant genotypes need to be bred by adopting objective and precise assessment of the disease ... | 2013 | 23955474 |
| a genome-wide survey of highly expressed non-coding rnas and biological validation of selected candidates in agrobacterium tumefaciens. | agrobacterium tumefaciens is a plant pathogen that has the natural ability of delivering and integrating a piece of its own dna into plant genome. although bacterial non-coding rnas (ncrnas) have been shown to regulate various biological processes including virulence, we have limited knowledge of how agrobacterium ncrnas regulate this unique inter-kingdom gene transfer. using whole transcriptome sequencing and an ncrna search algorithm developed for this work, we identified 475 highly expressed ... | 2013 | 23950988 |
| stable transformation of ferns using spores as targets: pteris vittata and ceratopteris thalictroides. | ferns (pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. we report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. spores of the arsenic hyperaccumulating fern pteris vittata and tetraploid 'c-fern express' (ceratopteris thalictroides) were stably transformed by agrobacterium tumefaciens with constructs c ... | 2013 | 23933990 |
| agrobacterium-mediated transformation of guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. | guignardia citricarpa is the causal agent of citrus black spot (cbs), an important disease in citriculture. due to the expressive value of this activity worldwide, especially in brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control cbs. this work describes for the first time an efficient and successful methodology for genetic transformation of g. citricarpa mycelia, which ... | 2013 | 23931121 |
| purple-leaved ficus lyrata plants produced by overexpressing a grapevine vvmyba1 gene. | this study established an efficient method of regenerating plants of ficus lyrata and producing purple-leaved f. lyrata plants through genetic transformation using a vvmyba1 gene of grapevine. | 2013 | 23926030 |
| the selectable antibiotic marker, teta(c), increases pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat. | the presence of the widely used selectable antibiotic marker, teta(c), unexpectedly increased the sensitivity of pseudomonas aeruginosa pao1 to the superoxide-generating herbicide, paraquat. a dna fragment spanning the first 99 amino acids of teta(c) was sufficient to confer paraquat sensitivity. the teta(c)-induced paraquat sensitive phenotype was observed in other gram-negative bacteria such as agrobacterium tumefaciens, salmonella enterica ser. typhimurium and xanthomonas campestris suggestin ... | 2013 | 23907582 |
| silencing agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance. | grapevine rootstock transformed with an agrobacterium oncogene-silencing transgene was resistant to certain agrobacterium strains but sensitive to others. thus, genetic diversity of agrobacterium oncogenes may limit engineering crown gall resistance. | 2013 | 23903949 |
| identification and characterization of the biochemical function of agrobacterium t-complex-recruiting protein atu5117. | atu5117 from agrobacterium tumefaciens is a highly conserved protein with a putative nucleotidyltransferase domain in its n-terminal region and a putative higher eukaryotes and prokaryotes nucleotide-binding domain in its c-terminal region. this protein has been shown to be a t-complex-recruiting protein that can recruit t-complex from the cytosol to the polar virb/d4 type iv secretion system (t4ss). however, the biochemical function of atu5117 is still unknown. here, we show that atu5117 is a ( ... | 2013 | 23902381 |
| norway spruce (picea abies) genetic transformation with modified cry3a gene of bacillus thuringiensis. | modified versions of the cry3a gene of bacillus thuringiensis (bt) were transferred into norway spruce (picea abies). both the biolistic approach and agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (et) cultures. the latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. the modified cry3a genes were driven by a 35s promoter and the nptii screenable selection mark ... | 2013 | 23888296 |
| construction of a sonchus yellow net virus minireplicon: a step toward reverse genetic analysis of plant negative-strand rna viruses. | reverse genetic analyses of negative-strand rna (nsr) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant nsr viruses. to develop a reverse genetic approach for analysis of plant nsr viruses, we have engineered sonchus yellow net nucleorhabdovirus (synv) minireplicon (mr) reporter cassettes for agrobacterium tumefaciens expression in nicotiana benthamiana leaves. fluorescent reporter ... | 2013 | 23885070 |
| inhibition of pbgp43 expression may suggest that gp43 is a virulence factor in paracoccidioides brasiliensis. | glycoprotein gp43 is an immunodominant diagnostic antigen for paracoccidioidomycosis caused by paracoccidioides brasiliensis. it is abundantly secreted in isolates such as pb339. it is structurally related to beta-1,3-exoglucanases, however inactive. its function in fungal biology is unknown, but it elicits humoral, innate and protective cellular immune responses; it binds to extracellular matrix-associated proteins. in this study we applied an antisense rna (arna) technology and agrobacterium t ... | 2013 | 23874627 |
| regeneration and agrobacterium-mediated transformation of the apomictic species eulaliopsis binata. | protocols for regeneration and agrobacterium-mediated transformation of the apomictic species eulaliopsis binata were developed. initially, seeds of four genotypes of e. binata were incubated on a callus induction murashige and skoog (ms) basal medium supplemented with three concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d). it was found that 36.2 % of explants developed highly friable callus on medium containing 3.0 mg l(-1) 2,4-d. based on frequency of callus induction, the genotype nei ... | 2013 | 23873641 |
| ectopic localization of auxin and cytokinin in tobacco seedlings by the plant-oncogenic ak-6b gene of agrobacterium tumefaciens ake10. | the oncogenic 6b gene of agrobacterium tumefaciens induces a number of morphological and metabolic alterations in plants. although molecular functions associated with the 6b genes have been proposed, including auxin transport, sugar transport, transcriptional regulation, and mirna metabolism, so far an unequivocal conclusion has not been obtained. we investigated the association between auxin accumulation and tumor development of the tobacco seedlings expressing the ak-6b gene under the control ... | 2013 | 23873395 |
| the glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast candida utilis strain nrrl y-660 is functional in agrobacterium tumefaciens. | the glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast candida utilis strain nrrl y-660 was cloned to create a novel integrative vector for agrobacterium tumefaciens-mediated transformation. the new binary vector harbors β-glucuronidase activity as reporter and kanamicin/geneticin resistance as selection marker. recombinant clones of a. tumefaciens show kanamycin resistance and high β-glucuronidase activity under the control of the c. utilis promoter. this finding can be explain ... | 2013 | 23873160 |
| independent activity of the homologous small regulatory rnas abcr1 and abcr2 in the legume symbiont sinorhizobium meliloti. | the legume symbiont sinorhizobium meliloti expresses a plethora of small noncoding rnas (srnas) whose function is mostly unknown. here, we have functionally characterized two tandemly encoded s. meliloti rm1021 srnas that are similar in sequence and structure. homologous srnas (designated abcr1 and abcr2) have been shown to regulate several abc transporters in the related α-proteobacteria agrobacterium tumefaciens and brucella abortus. in rm1021, abcr1 and abcr2 exhibit divergent unlinked regula ... | 2013 | 23869210 |
| transformation of ltp gene into brassica napus to enhance its resistance to sclerotinia sclerotiorum. | rapeseed (brassica napus l.) is one of the most important economic crops worldwide, and sclerotinia sclerotiorum is the most dangerous disease that affects its yield greatly. lipid transfer protein (ltp) has broad-spectrum anti-bacterial and fungal activities. in this study, b. napus was transformed using agrobacterium tumefaciens harboring the plasmid-containing ltp gene to study its possible capability of increasing plant's resistance. first, we optimized the petiole genetic transformation sys ... | 2013 | 23866620 |
| systematic dissection of the agrobacterium type vi secretion system reveals machinery and secreted components for subcomplex formation. | the type vi secretion system (t6ss) is widely distributed in pathogenic proteobacteria. sequence and structural analysis of t6ss reveals a resemblance to the t4 bacteriophage tail, in which an outer sheath structure contracts an internal tube for injecting nucleic acid into bacterial cells. however, the molecular details of how this phage tail-like t6ss structure is assembled in vivo and executed for exoprotein or effector secretion remain largely unknown. here, we used a systematic approach to ... | 2013 | 23861778 |
| plant biotechnology for food security and bioeconomy. | this year is a special year for plant biotechnology. it was 30 years ago, on january 18 1983, one of the most important dates in the history of plant biotechnology, that three independent groups described agrobacterium tumefaciens-mediated genetic transformation at the miami winter symposium, leading to the production of normal, fertile transgenic plants (bevan et al. in nature 304:184-187, 1983; fraley et al. in proc natl acad sci usa 80:4803-4807, 1983; herrera-estrella et al. in embo j 2:987- ... | 2013 | 23860797 |
| homoserine catabolism by rhizobium leguminosarum bv. viciae 3841 requires a plasmid-borne gene cluster that also affects competitiveness for nodulation. | homoserine represents a substantial component of pea root exudate that may be important for plant-microbe interactions in the rhizosphere. we identified a gene cluster on plasmid prl8ji that is required for homoserine utilization by rhizobium leguminosarum bv. viciae. the genes are arranged as two divergently expressed predicted operons that were induced by l-homoserine, pea root exudate, and were expressed on pea roots. a mutation in gene prl80083 that prevented utilization of homoserine as a s ... | 2014 | 23859230 |
| metalloprotease genes of trichophyton mentagrophytes are important for pathogenicity. | metalloproteases (mep) of the m36 family are important virulence factors for the host invasion by the dermatophyte trichophyton mentagrophytes. dermatophytes secrete keratinase to degrade human and animal keratin and invade the skin. in previous studies, primers designed from the mep gene sequences of aspergillus fumigatus and a. oryzae were used to amplify the mep genes from t. mentagrophytes, and the five mep genes (mep1-mep5) were expressed. differences in the expression of these five mep gen ... | 2014 | 23859078 |
| the transformation of the photo-thermo sensitive genic male-sterile line 261s of rice via an expression vector containing the anti-waxy gene. | transgenic photo-thermo sensitive genic male sterility oryza sativa l. cv. "261s" plants with the anti-waxy gene were successfully obtained using an agrobacterium tumefaciens-mediated co-transformation method. marker-free homozygous transgenic lines with the anti-waxy gene were obtained. the setting seed rates of the transgenic plants via self-pollination or via crossing with the restorer line wx99075 rice and the 1000-grain weight of the transgenic plants and the f2 hybrid seeds obtained by cro ... | 2013 | 23853508 |
| assessment of factors influencing the agrobacterium-mediated in planta seed transformation of brinjal (solanum melongena l.). | an efficient and reproducible in planta transformation method was developed for brinjal using seed as an explant. the brinjal seeds were infected with agrobacterium tumefaciens eha 105 harbouring pcambia 1301-bar plasmid, and the transformants were selected against basta®. several parameters influencing the in planta seed transformation such as pre-culture duration, acetosyringone concentration, surfactants, duration of sonication, vacuum pressure and vacuum duration have been evaluated. the put ... | 2013 | 23852797 |
| a genetic system for citrus tristeza virus using the non-natural host nicotiana benthamiana: an update. | in nature citrus tristeza virus (ctv), genus closterovirus, infects only the phloem cells of species of citrus and related genera. finding that the ctv t36 strain replicated in nicotiana benthamiana (nb) protoplasts and produced normal virions allowed development of the first genetic system based on protoplast transfection with rna transcribed from a full-genome cdna clone, a laborious and uncertain system requiring several months for each experiment. we developed a more efficient system based o ... | 2013 | 23847598 |
| genetic transformation of western clover (trifolium occidentale d. e. coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species. | western clover (trifolium occidentale) is a perennial herb with characteristics compatible for its development as an attractive model species for genomics studies relating to the forage legume, white clover (trifolium repens). its characteristics such as a small diploid genome, self-fertility and ancestral contribution of one of the genomes of t. repens, facilitates its use as a model for genetic analysis of plants transformed with legume or novel genes. | 2013 | 23841995 |
| genetic analysis of agrobacterium tumefaciens unipolar polysaccharide production reveals complex integrated control of the motile-to-sessile switch. | many bacteria colonize surfaces and transition to a sessile mode of growth. the plant pathogen agrobacterium tumefaciens produces a unipolar polysaccharide (upp) adhesin at single cell poles that contact surfaces. here we report that elevated levels of the intracellular signal cyclic diguanosine monophosphate (c-di-gmp) lead to surface-contact-independent upp production and a red colony phenotype due to production of upp and the exopolysaccharide cellulose, when a. tumefaciens is incubated with ... | 2013 | 23829710 |
| protease inhibitor from insect silk-activities of derivatives expressed in vitro and in transgenic potato. | several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (spi2), which is a silk component in galleria mellonella (lepidoptera, insecta), were prepared in the expression vector pichia pastoris. both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase k. the synthetic spi2 gene with ala codon in the p1 position was fused with mgfp-5 to facilitate detection of the transgene and its protein product. a construct ... | 2013 | 23824530 |
| expression of verocytotoxic escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model. | verocytotoxic escherichia (e.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. the production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. this study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic e. coli strain. parameters related to an immunological response and pos ... | 2013 | 23820163 |
| central venous catheter infections in outpatients with pulmonary hypertension treated with continuous iloprost. | intravenous prostanoid therapy is one cornerstone of therapy for patients with pre-capillary pulmonary hypertension (ph). long-term central venous catheters expose patients to infectious complications. | 2013 | 23817035 |
| identification of virulence genes in the crucifer anthracnose fungus colletotrichum higginsianum by insertional mutagenesis. | to investigate the molecular and genetic mechanisms underlying virulence of colletotrichum higginsianum on arabidopsis thaliana, a t-dna insertion mutant library of c. higginsianum, the causal agent of crucifer anthracnose, was established using agrobacterium tumefaciens-mediated transformation. among 875 transformants tested for virulence on arabidopsis, six mutants with altered virulence, including an appressorial melanin-deficient mutant t734, two mutants defective in penetration, t45 and b30 ... | 2013 | 23806215 |
| the role of fabg3 in fruit ripening and b. cinerea fungal infection of strawberry. | in plants, β-glucosidases (bg) have been implicated in developmental and pathogen defense, and are thought to take part in abscisic acid (aba) synthesis via hydrolysis of aba glucose ester to release active aba; however, there is no genetic evidence for the role of bg genes in ripening and biotic/abiotic stress in fruits. to clarify the role of bg genes in fruit, eight fa/fvbg genes encoding β-glucosidase were isolated using information from the genbank strawberry nucleotide database. of the fa/ ... | 2013 | 23802911 |
| taxol production by an endophytic fungus, fusarium redolens, isolated from himalayan yew. | different endophytic fungi isolated from himalayan yew plants were tested for their ability to produce taxol. the bapt gene (c-13 phenylpropanoid side chain-coa acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi. taxol extracted from fungal strain tbpj-b was identified by hplc and ms analysis. strain tbpj-b was identified as fusarium redolens based on the morphology and internal transcribed spacer region of nrd ... | 2013 | 23801250 |
| expression of shigella flexneri ipab gene in tobacco. | shigellosis is a leading cause of diarrhea in many developing countries and although the disease can be controlled and managed with antibiotics, the constant emergence of resistant species requiring ever newer antibacterial drugs make development of an effective vaccine necessary. the bacteria are highly contagious and since immunity to shigella is serotype-specific a multi-serotype vaccine is required for adequate protection. proteins encoded by shigella invasion plasmid, which are part of the ... | 2013 | 23799180 |
| improving the glycosyltransferase activity of agrobacterium tumefaciens glycogen synthase by fusion of n-terminal starch binding domains (sbds). | glycogen and starch, the major storage carbohydrate in most living organisms, result mainly from the action of starch or glycogen synthases (ss or gs, respectively, ec 2.4.1.21). ssiii from arabidopsis thaliana is an ss isoform with a particular modular organization: the c-terminal highly conserved glycosyltransferase domain is preceded by a unique specific region (ssiii-sd) which contains three in tandem starch binding domains (sbds, named d1, d2 and d3) characteristic of polysaccharide degradi ... | 2013 | 23796574 |
| in vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in turkey. | to investigate antibacterial and antitumor activities of 51 different extracts prepared with 3 types of solvents (water, ethanol and methanol) of 16 different plant species (ajuga reptans (a. reptans) l., phlomis pungens (p. pungens) willd., marrubium astracanicum (m. astracanicum) jacq., nepeta nuda (n. nuda) l., stachys annua (s. annua) l., genista lydia (g. lydia) boiss., nuphar lutea (n. lutea) l., nymphaea alba (n. alba) l., vinca minor (v. minor) l., stellaria media (s. media) l., capsella ... | 2013 | 23790332 |
| large deletions in the patc58 megaplasmid of agrobacterium tumefaciens can confer reduced carriage cost and increased expression of virulence genes. | the accessory plasmid patc58 of the common laboratory strain of agrobacterium tumefaciens confers numerous catabolic functions and has been proposed to play a role in virulence. genomic sequencing of evolved laboratory strains of a. tumefaciens revealed the presence of multiple deletion events in the at plasmid, with reductions in plasmid size ranging from 25% to 30% (115-194 kb). flanking both ends of the sites of these deletions is a short-nucleotide repeat sequence that is in a single copy in ... | 2013 | 23783172 |
| [infective endocarditis by rhizobium radiobacter. a case report]. | rhizobium radiobacter is a gram-negative, nitrogen-fixing bacterium, which is found mainly on the ground. it rarely causes infections in humans. it has been associated with bacteremia, secondary to colonization of intravascular catheters, in immunocompromised patients. the aim of this paper was to report the case of an infective endocarditis caused by r. radiobacter, in a 47-year-old male, diagnosed with chronic kidney disease stage 5, on replacement therapy with hemodialysis and who attended th ... | 2013 | 23781714 |
| coral-associated bacteria, quorum sensing disrupters, and the regulation of biofouling. | marine biofouling, the settlement of microorganisms and macroorganisms on structures submerged in seawater, although economically detrimental, is a successful strategy for survival in hostile environments, where coordinated bacterial communities establish biofilms via the regulation of quorum sensing (qs) communication systems. the inhibition of qs activity among bacteria isolated from different coral species was investigated to gain further insight into its potency in the attenuation, or even t ... | 2013 | 23777289 |
| the thuefgkab operon of rhizobia and agrobacterium tumefaciens codes for transport of trehalose, maltitol, and isomers of sucrose and their assimilation through the formation of their 3-keto derivatives. | the thu operon (thuefgkab) in sinorhizobium meliloti codes for transport and utilization functions of the disaccharide trehalose. sequenced genomes of members of the rhizobiaceae reveal that some rhizobia and agrobacterium possess the entire thu operon in similar organizations and that mesorhizobium loti maff303099 lacks the transport (thuefgk) genes. in this study, we show that this operon is dedicated to the transport and assimilation of maltitol and isomers of sucrose (leucrose, palatinose, a ... | 2013 | 23772075 |
| agrobacterium tumefaciens-mediated genetic transformation of salix matsudana koidz. using mature seeds. | an agrobacterium tumefaciens-mediated transformation method was developed for salix matsudana koidz. using mature seeds as starting material. multiple shoots were induced directly from embryonic shoot apices of germinating seeds. although thidiazuron, 6-benzylaminopurine and zeatin induced multiple shoot induction with high frequency, zeatin (4.5 μm) was more effective for elongation of shoots and roots. the binary vector pcambia1303, which contained neomycin phosphotransferase as a selectable m ... | 2013 | 23771952 |
| efficient auto-excision of a selectable marker gene from transgenic citrus by combining the cre/loxp system and ipt selection. | a highly efficient cre-mediated deletion system, offering a good alternative for producing marker-free transgenic plants that will relieve public concerns regarding gmos, was first developed in citrus. the presence of marker genes in genetically modified crops raises public concerns regarding their safety. the removal of marker genes can prevent the risk of their flow into the environment and hasten the public's acceptance of transgenic products. in this study, a new construct based on the cre/l ... | 2013 | 23771575 |
| variable internal flexibility characterizes the helical capsid formed by agrobacterium vire2 protein on single-stranded dna. | agrobacterium is known for gene transfer to plants. in addition to a linear ssdna oligonucleotide, agrobacterium tumefaciens secretes an abundant ssdna-binding effector, vire2. in many ways vire2 adapts the conjugation mechanism to transform the eukaryotic host. the crystal structure of vire2 shows two compact domains joined by a flexible linker. bound to ssdna, vire2 forms an ordered solenoidal shell, or capsid known as the t-complex. here, we present a three-dimensional reconstruction of the v ... | 2013 | 23769668 |
| novel rhizobium lineages isolated from root nodules of the common bean (phaseolus vulgaris l.) in andean and mesoamerican areas. | the taxonomic affiliations of nineteen root-nodule bacteria isolated from the common bean (phaseolus vulgaris l.) in mexico, ecuador and brazil were investigated by analyses of 16s rrna and of four protein-coding housekeeping genes. one strain from mexico could be assigned to rhizobium etli and two from brazil to rhizobium leucaenae, whereas another from mexico corresponded to a recently described bean-nodulating species-level lineage related to r. etli and rhizobium phaseoli. ten strains isolat ... | 2013 | 23764913 |
| impact of cagpai and t4ss on the inflammatory response of human neutrophils to helicobacter pylori infection. | helicobacter pylori contains a pathogenicity island, cagpai, with genes homologous to components of the type iv secretion system (t4ss) of agrobacterium tumefaciens. the t4ss components assemble a structure that transfers caga protein and peptidoglycan into host epithelial cells, causing the increased release of interleukin 8 (il8) from the cells. the toll-like receptors on neutrophils recognize h. pylori, initiating signaling pathways that enhance the activation of nf-κb. however, the roles of ... | 2014 | 23755130 |
| agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane. | an efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant. transgenic sugarcane production through agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. to obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were infected with agrobacterium tumefaciens eha 105 harboring pcambia 1304-bar and transformed plants we ... | 2013 | 23749098 |
| overexpression of the glutamine synthetase gene modulates oxidative stress response in rice after exposure to cadmium stress. | overexpression of osgs gene modulates oxidative stress response in rice after exposure to cadmium stress. our results describe the features of transformants with enhanced tolerance to cd and abiotic stresses. glutamine synthetase (gs) (ec 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. exposure of plants to cadmium (cd) has been reported to decrease gs activity in maize, pea, bean, and ric ... | 2013 | 23743654 |
| n-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures. | quorum sensing (qs) systems, which depend on n-acylhomoserine lactone (ahl) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. one hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic ahls [n-butyryl-dl-homoserine lactone (c4-hsl), n-hexanoyl-dl-homoserine lactone (c6-hsl), n-octanoyl-dl-homoserine lactone (c8-hsl), n-decanoyl-dl-homoserine lactone (c10-hsl) and n-dodeca ... | 2013 | 23743010 |
| the helicobacter pylori protein cagm is located in the transmembrane channel that is required for caga translocation. | helicobacter pylori (h. pylori) is a human gastric pathogen that colonizes the stomach in more than 50 % of the world's human population. infection with this bacterium can induce several gastric diseases ranging from gastritis to peptic ulcer and gastric cancer. virulent h. pylori isolates harboring the cag pathogenicity island (cag pai), which encodes a type iv secretion system (t4ss), form a pilus for the injection of its major virulence protein caga into gastric cells. several cag pai genes h ... | 2013 | 23736225 |
| brucella bior regulator defines a complex regulatory mechanism for bacterial biotin metabolism. | the enzyme cofactor biotin (vitamin h or b7) is an energetically expensive molecule whose de novo biosynthesis requires 20 atp equivalents. it seems quite likely that diverse mechanisms have evolved to tightly regulate its biosynthesis. unlike the model regulator bira, a bifunctional biotin protein ligase with the capability of repressing the biotin biosynthetic pathway, bior has been recently reported by us as an alternative machinery and a new type of gntr family transcriptional factor that ca ... | 2013 | 23729648 |
| botrytis cinerea chitin synthase bcchsvi is required for normal growth and pathogenicity. | fungal chitin synthase of classes v and vi (or vii), which contain an additional n-terminal myosin motor domain, have been shown to play important roles in pathogenesis. to study the function of bcchsvi in botrytis cinerea, bcchs6 gene was disrupted through agrobacterium tumefaciens-mediated transformation. the bcchs6 disruption mutant exhibited a 45.5 % increasing in its chitin content when compared with wild strain. the qrt-pcr analysis revealed that in bcchs6 mutant the expression of bcchs6 w ... | 2013 | 23722656 |
| l-glutamine and l-glutamic acid facilitate successful agrobacterium infection of recalcitrant tea cultivars. | the first step in agrobacterium tumefaciens infection of plants is the establishment of cell-cell contact between the two partners. however, failure to establish such contact makes many plants and explants recalcitrant to a. tumefaciens infection. tea is one such example where even the popular inducer, acetosyringone failed to facilitate a. tumefaciens infection due to the presence of high amounts of bactericidal/bacteriostatic polyphenols. quinones are formed as a result of polyphenols oxidatio ... | 2013 | 23712792 |
| in planta fitness-cost of the atu4232-regulon encoding for a selective gaba-binding sensor in agrobacterium. | gaba (gamma-aminobutyric acid) mediates cell-to-cell communication in eukaryotes and interspecies communication in host-microbe interactions. agrobacterium tumefaciens induces the development of plant tumor in which gaba accumulates. two periplasmic binding proteins atu2422 and atu4243 and their appropriate abc-transporter are involved in the binding and importation of gaba. the structure of the selective gaba-binding atu4243 reveals a gaba conformation similar to a proposed model of gaba bound ... | 2013 | 23710277 |
| investigation of the fusarium virguliforme fvtox1 mutants revealed that the fvtox1 toxin is involved in foliar sudden death syndrome development in soybean. | the soil borne fungus, fusarium virguliforme, causes sudden death syndrome (sds) in soybean, which is a serious foliar and root rot disease. the pathogen has never been isolated from the diseased foliar tissues; phytotoxins produced by the pathogen are believed to cause foliar sds symptoms. one of these toxins, a 13.5-kda acidic protein named fvtox1, has been hypothesized to interfere with photosynthesis in infected soybean plants and cause foliar sds. the objective of this study is to determine ... | 2013 | 23702608 |
| implantable vascular access port-associated bloodstream infection caused by rhizobium radiobacter: a case report. | rhizobium radiobacter is an uncommon opportunistic pathogen present in soil. it has been particularly associated with indwelling intravascular devices in immunocompromised patients. reported herein is a case of r. radiobacter bloodstream infection associated with an implantable vascular access port, which was easily controlled with antimicrobial treatment and did not require removal of the intravascular device, in a child diagnosed with acute lymphoblastic leukemia. also included is a review of ... | 2013 | 23692845 |
| a flexible c-terminal linker is required for proper ftsz assembly in vitro and cytokinetic ring formation in vivo. | assembly of the cytoskeletal protein ftsz into a ring-like structure is required for bacterial cell division. structurally, ftsz consists of four domains: the globular n-terminal core, a flexible linker, 8-9 conserved residues implicated in interactions with modulatory proteins, and a highly variable set of 4-10 residues at its very c terminus. largely ignored and distinguished by lack of primary sequence conservation, the linker is presumed to be intrinsically disordered. here we employ genetic ... | 2013 | 23692518 |
| transient expression of cor gene in papaver somniferum. | papaver somniferum is the commercial source of morphine and codeine. the isolation of effective genes involved in the morphine biosynthesis of p. somniferum is very important in the production of specific metabolites achieved using metabolic engi-neering techniques. in this pathway, the key enzyme cor is involved in the conversion of codeinone to codeine and morphinone to morphine. | 2011 | 23678433 |
| synthesis and antitumor activity of substituted succinamides using a potato disc tumor induction assay. | in view of potential biological activities of some succinic acid derivatives, we synthesized some novel n-[4-(4-morpholinosulfonyl)-phenyl]-succinamides (6a, c; 7a, c) and n-[4-(benzylaminosulfonyl) phenyl]-succinamides (6b, d; 7b, d) derivatives as antitumor agents. the antitumor activity of compounds was studied using the potato disk bioassay technique. vincristine at 0.25 mg/ml was employed as positive control and caused -67.24% inhibitions. compound 7b at 1 mg/ml caused -80.50% tumor inhibit ... | 2007 | 23675023 |
| dynamic ftsa and ftsz localization and outer membrane alterations during polar growth and cell division in agrobacterium tumefaciens. | growth and cell division in rod-shaped bacteria have been primarily studied in species that grow predominantly by peptidoglycan (pg) synthesis along the length of the cell. rhizobiales species, however, predominantly grow by pg synthesis at a single pole. here we characterize the dynamic localization of several agrobacterium tumefaciens components during the cell cycle. first, the lipophilic dye fm 4-64 predominantly stains the outer membranes of old poles versus growing poles. in cells about to ... | 2013 | 23674672 |
| rhizobium radiobacter peritonitis revisited: catheter removal is not mandatory. | 2015 | 23660610 | |
| promoting student involvement with environmental laboratory experiments in a general microbiology course. | this is a descriptive study of a series of laboratory exercises on environmental microbiology carried out by students in a general microbiology course during eight of the twelve weeks of the semester. the revised laboratory component is predicated upon seawater and sediment samples collected by student pairs using marine sampling equipment on a field trip aboard a research vessel. two longitudinal studies were performed: assay for antibiotic production from isolated actinomycetes and constructio ... | 2003 | 23653550 |
| fast induction of biosynthetic polysaccharide genes lpxa, lpxe, and rkpi of rhizobium sp. strain prf 81 by common bean seed exudates is indicative of a key role in symbiosis. | rhizobial surface polysaccharides (sps) are, together with nodulation (nod) factors, recognized as key molecules for establishment of rhizobia-legume symbiosis. in rhizobium tropici, an important nitrogen-fixing symbiont of common bean (phaseolus vulgaris l.), molecular structures and symbiotic roles of the sps are poorly understood. in this study, rhizobium sp. strain prf 81 genes, belonging to the r. tropici group, were investigated: lpxa and lpxe, involved in biosynthesis and modification of ... | 2013 | 23652766 |
| the methylation of the pcmyb10 promoter is associated with green-skinned sport in max red bartlett pear. | varieties of the european pear (pyrus communis) can produce trees with both red- and green-skinned fruits, such as the max red bartlett (mrb) variety, although little is known about the mechanism behind this differential pigmentation. in this study, we investigated the pigmentation of mrb and its green-skinned sport (mrb-g). the results suggest that a reduction in anthocyanin concentration causes the mrb-g sport. transcript levels of pcufgt (for udp-glucose:flavonoid 3-o-glucosyltransferase), th ... | 2013 | 23629835 |
| [genetic transformation of pinellia ternata with agrobacterium tumefaciens-mediated shsp genes]. | to establish an efficient genetic transformation system of pinellia ternata. | 2012 | 23627174 |
| a putative transmembrane leucine zipper of agrobacterium virb10 is essential for t-pilus biogenesis but not type iv secretion. | the agrobacterium tumefaciens virb/vird4 type iv secretion system is composed of a translocation channel and an extracellular t pilus. bitopic virb10, the virb7 lipoprotein, and virb9 interact to form a cell envelope-spanning structural scaffold termed the "core complex" that is required for the assembly of both structures. the related pkm101-encoded core complex is composed of 14 copies each of these virb homologs, and the transmembrane (tm) α helices of virb10-like traf form a 55-å-diameter ri ... | 2013 | 23625845 |
| a rapid assay to quantify the cleavage efficiency of custom-designed nucleases in planta. | custom-designed nucleases are a promising technology for genome editing through the catalysis of double-strand dna breaks within target loci and subsequent repair by the host cell, which can result in targeted mutagenesis or gene replacement. implementing this new technology requires a rapid means to determine the cleavage efficiency of these custom-designed proteins in planta. here we present such an assay that is based on cleavage-dependent luciferase gene correction as part of a transient dua ... | 2013 | 23625357 |
| agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana. | cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ... | 2013 | 23623981 |
| chloroplast-targeted expression of the codon-optimized truncated cry1ah gene in transgenic tobacco confers a high level of protection against insects. | the study of insect-resistant transgenic tobacco provides a good foundation for the further application of the cry1ah gene in other important crops. to improve transgene expression levels and insect resistance, the coding sequence of the novel bacillus thuringiensis insecticidal gene cry1ah (truncated cry1ah) was modified according to the codon bias of the plant by increasing its gc content from the original 37 % to 48, 55, and 63 % (designated m1-cry1ah, m2-cry1ah, and m3-cry1ah, respectively). ... | 2013 | 23620344 |
| high expression of a neutral endo-β-glucanase gene from humicola insolens in trichoderma reesei. | the neutral endo-β-glucanase gene cel5a from humicola insolens was cloned and connected with the cellobiohydrolase 1 promoter from trichoderma reesei to construct a recombinant plasmid pcb-heg with the hygromycin b resistance marker. the plasmid was introduced into conidia of t. reesei using the agrobacterium tumefaciens mediated transformation method. eight transformants were obtained on screening plates with sodium carboxymethyl cellulose as the sole carbon source. stable integration of the ce ... | 2013 | 23619969 |
| shape-dependent bactericidal activity of tio2 for the killing of gram-negative bacteria agrobacterium tumefaciens under uv torch irradiation. | this paper demonstrated the relative bactericidal activity of photoirradiated (6w-uv torch, λ > 340 nm and intensity = 0.64 mw/cm(2)) p25-tio2 nanoparticles, nanorods, and nanotubes for the killing of gram-negative bacterium agrobacterium tumefaciens lba4404 for the first time. tio2 nanorod (anatase) with length of 70-100 nm and diameter of 10-12 nm, and tio2 nanotube with length of 90-110 nm and diameter of 9-11 nm were prepared from p-25 degussa tio2 (size, 30-50 nm) by hydrothermal method and ... | 2013 | 23608987 |
| booster responses by oral vaccination with transgenic plants against chicken leucocytozoonosis. | we developed a transgenic potato (trp/r7) expressing the recombinant r7 (rr7) antigen for use as an oral vaccine to protect against a chicken protozoan disease, chicken leucocytozoonosis. the trp/r7 potato was produced by agrobacterium tumefaciens-mediated transformation and regeneration, and the r7 gene insertion into potato chromosomes was confirmed by genomic polymerase chain reaction and southern hybridization. rr7 antigen expression in trp/r7 potato was also confirmed by sandwich enzyme-lin ... | 2013 | 23607580 |
| the structure of glycerol trinitrate reductase nera from agrobacterium radiobacter reveals the molecular reason for nitro- and ene-reductase activity in oye homologues. | in recent years, old yellow enzymes (oyes) and their homologues have found broad application in the efficient asymmetric hydrogenation of activated c=c bonds with high selectivities and yields. members of this class of enzymes have been found in many different organisms and are rather diverse on the sequence level, with pairwise identities as low as 20 %, but they exhibit significant structural similarities with the adoption of a conserved (αβ)(8)-barrel fold. some oyes have been shown not only ... | 2013 | 23606302 |
| biodegradation of nicotine by a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35 and its new biotransformation intermediates. | tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. in the investigation, a novel nicotine-biodegrading bacterium tnd35 was isolated and identified as pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16s rrna sequence homology. we have studied the nicotine biodegradation potential of strain tnd35 by detecting the intermediate metabolites using an array of approaches such as hplc, gc-ms, nmr and ft-ir. biotransform ... | 2014 | 23604517 |
| identifying pathogenicity genes in the rubber tree anthracnose fungus colletotrichum gloeosporioides through random insertional mutagenesis. | to gain more insight into the molecular mechanisms of colletotrichum gloeosporioides pathogenesis, agrobacterium tumefaciens-mediated transformation (atmt) was used to identify mutants of c. gloeosporioides impaired in pathogenicity. an atmt library of 4128 c. gloeosporioides transformants was generated. transformants were screened for defects in pathogenicity with a detached copper brown leaf assay. 32 mutants showing reproducible pathogenicity defects were obtained. southern blot analysis show ... | 2013 | 23602122 |
| identification of a genomic region containing a novel promoter resistant to glucose repression and over-expression of β-glucosidase gene in hypocrea orientalis eu7-22. | a high concentration of glucose in the medium could greatly inhibit the expression of cellulase in filamentous fungi. the aspartic protease from fungus hypocrea orientalis eu7-22 could efficiently express under both induction condition and glucose repression condition. based on the sequence of structure gene of aspartic protease, the upstream sequence harboring the putative promoter proa for driving the expression of aspartic protease was obtained by genome walking. the upstream sequence contain ... | 2013 | 23594998 |
| effect of plant phenolic compounds on biofilm formation by pseudomonas aeruginosa. | in the natural environment, bacteria predominantly exist in matrix-enclosed multicellular communities associated with various surfaces, referred to as biofilms. bacteria in biofilms are extremely resistant to antibacterial agents thus causing serious problems for antimicrobial therapy. in this study, we showed that different plant phenolic compounds, at concentrations that did not or weakly suppressed bacterial growth, increased the capacity of pseudomonas aeruginosa pao1 to form biofilms. biofi ... | 2013 | 23594262 |
| the tzs protein and exogenous cytokinin affect virulence gene expression and bacterial growth of agrobacterium tumefaciens. | the soil phytopathogen agrobacterium tumefaciens causes crown gall disease in a wide range of plant species. the neoplastic growth at the infection sites is caused by transferring, integrating, and expressing transfer dna (t-dna) from a. tumefaciens into plant cells. a trans-zeatin synthesizing (tzs) gene is located in the nopaline-type tumor-inducing plasmid and causes trans-zeatin production in a. tumefaciens. similar to known virulence (vir) proteins that are induced by the vir gene inducer a ... | 2013 | 23593941 |
| crystal structure of a prokaryotic (6-4) photolyase with an fe-s cluster and a 6,7-dimethyl-8-ribityllumazine antenna chromophore. | the (6-4) photolyases use blue light to reverse uv-induced (6-4) photoproducts in dna. this (6-4) photorepair was thought to be restricted to eukaryotes. here we report a prokaryotic (6-4) photolyase, phrb from agrobacterium tumefaciens, and propose that (6-4) photolyases are broadly distributed in prokaryotes. the crystal structure of photolyase related protein b (phrb) at 1.45 å resolution suggests a dna binding mode different from that of the eukaryotic counterparts. a his-his-x-x-arg motif i ... | 2013 | 23589886 |
| nitrite-mediated synthesis of chiral epichlorohydrin using halohydrin dehalogenase from agrobacterium radiobacter ad1. | in the current study, the haloalcohol dehalogenase hhec gene from agrobacterium radiobacter ad1 was synthesized and expressed in escherichia coli. after purification using ni-nitrilotriacetic acid affinity chromatography, hhec was used in the synthesis of chiral epichlorohydrin in the presence of no₂⁻. the optimal ph, temperature, and no₂⁻ concentration for enantioselectivity are 5.0, 37°c, and 60 mm, respectively. the maximum velocity and michaelis constant values for (s)-epichlorohydrin are 71 ... | 2012 | 23586826 |
| rapid and accurate species and genomic species identification and exhaustive population diversity assessment of agrobacterium spp. using reca-based pcr. | agrobacteria are common soil bacteria that interact with plants as commensals, plant growth promoting rhizobacteria or alternatively as pathogens. indigenous agrobacterial populations are composites, generally with several species and/or genomic species and several strains per species. we thus developed a reca-based pcr approach to accurately identify and specifically detect agrobacteria at various taxonomic levels. specific primers were designed for all species and/or genomic species of agrobac ... | 2013 | 23578959 |
| an experimentally tested scenario for the structural evolution of eukaryotic cys2his2 zinc fingers from eubacterial ros homologs. | the exact evolutionary origin of the zinc finger (zf) domain is unknown, as it is still not clear from which organisms it was first derived. however, the unique features of the zf domains have made it very easy for evolution to tinker with them in a number of different manners, including their combination, variation of their number by unequal crossing-over or tandem duplication and tuning of their affinity for specific dna sequence motifs through point substitutions. classical cys2his2 zf domain ... | 2013 | 23576569 |
| recent advances in the understanding of agrobacterium rhizogenes-derived genes and their effects on stress resistance and plant metabolism. | it is commonly accepted that the plant pathogens agrobacterium rhizogenes and agrobacterium tumefaciens, acting via their t-dna oncogenes, disturb hormone metabolism or hormone perception pathways in plants, thereby attaining their aim of successful pathogenesis. in this work, we summarize recent data on the a. rhizogenes rolc and rolb oncogenes in comparison to the a. tumefaciens 6b oncogene with respect to their effects on the physiology of transformed cells. the newly discovered functions of ... | 2013 | 23576052 |
| tissue culture independent transformation of the forage crop sunnhemp (crotalaria juncea l.): an easy method towards generation of transgenics. | a transformation system which is free of in vitro plant regeneration following agrobacterium infection is established for the forage legume, sunnhemp (crotalaria juncea l.) where in the entire embryo axis of the germinating seed was used as the target tissue for transformation. after standardization of transformation conditions, the cotyledonary node of the embryo axis was infected with agrobacterium host lba 4404 harboring the recombinant vector pcambia 2301. the bivalent 1d gene of the two maj ... | 2011 | 23573040 |
| genetic transformation of indian isolate of lemna minor mediated by agrobacterium tumefaciens and recovery of transgenic plants. | transgenic plants of an indian isolate of lemna minor have been developed for the first time using agrobacterium tumefaciens and hard nodular cell masses 'nodular calli' developed on the bap - pretreated daughter frond explants in b5 medium containing sucrose (1.0 %) with 2,4-d (5.0 μm) and 2-ip (50.0 μm) or 2,4-d (50.0 μm) and tdz (5.0 μm) under light conditions. these calli were co-cultured with a. tumefaciens strain eha105 harboring a binary vector that contained genes for β-glucuronidase wit ... | 2011 | 23573002 |
| agrobacterium-mediated transformation in chickpea (cicer arietinum l.) with an insecticidal protein gene: optimisation of different factors. | agrobacterium-mediated transformation in chickpea was developed using strain lba4404 carrying nptii, uida and cryiac genes and transformants selected on murashige and skoog's basal medium supplemented with benzyladenine, kinetin and kanamycin. integration of transgenes was demonstrated using polymerase chain reaction and southern blot hybridization of t0 plants. the expression of cryiac delta endotoxin and gus enzyme was shown by enzyme linked immunosorbent assay and histochemical assay respecti ... | 2010 | 23572977 |
| a reliable protocol for transformation of catharanthus roseus through agrobacterium tumefaciens. | proliferation of axillary shoot buds and multiple shoot formation in catharanthus roseus was obtained in 96 % explants on ms medium (3 % sucrose) containing naa + ba. 2,4-d induced callusing in both, the nodal as well as in leaf segments. leaf-derived callus was used for transformation with agrobacterium tumefaciens lba4404/pbi-s1. bacterial cell concentration, duration of co-cultivation and acetosyringone concentration influenced transformation efficiency. under optimal co-cultivation condition ... | 2009 | 23572917 |
| tissue specific response of agrobacterium tumefaciens attachment to sorghum bicolor (l) moench. | agrobacterium mediated genetic transformation of plants have advantages over other methods, especially for making single copy transgenic plants with reduced chances of gene silencing and instability. however, monocotyledonous plant species could not utilize the full potential of this system because of possible limitations in agrobacterium interaction with monocot plant cells. agrobacterium attachment as a factor in genetic transformation was studied in the leaf, shoot apex, and leaf derived call ... | 2008 | 23572896 |
| tissue specific response of agrobacterium tumefaciens attachment to sorghum bicolor (l) moench. | agrobacterium mediated genetic transformation of plants have advantages over other methods, especially for making single copy transgenic plants with reduced chances of gene silencing and instability. however, monocotyledonous plant species could not utilize the full potential of this system because of possible limitations in agrobacterium interaction with monocot plant cells. agrobacterium attachment as a factor in genetic transformation was studied in the leaf, shoot apex, and leaf derived call ... | 2008 | 23572896 |
| an active ac/ds transposon system for activation tagging in tomato cultivar m82 using clonal propagation. | tomato (solanum lycopersicum) is a model organism for solanaceae in both molecular and agronomic research. this project utilized agrobacterium tumefaciens transformation and the transposon-tagging construct activator (ac)/dissociator (ds)-atag-bar_gosgfp to produce activation-tagged and knockout mutants in the processing tomato cultivar m82. the construct carried hygromycin resistance (hyg), green fluorescent protein (gfp), and the transposase (tpase) of maize (zea mays) activator major transcri ... | 2013 | 23569107 |
| prediction of candidate small non-coding rnas in agrobacterium by computational analysis. | small non-coding rnas with important regulatory roles are not confined to eukaryotes. recent work has uncovered a growing number of bacterial small rnas (srnas), some of which have been shown to regulate critical cellular processes. computational approaches, in combination with molecular experiments, have played an important role in the identification of these srnas. at present, there is no information on the presence of small non-coding rnas and their genes in the agrobacterium tumefaciens geno ... | 2010 | 23554609 |
| bacteremia caused by rhizobium radiobacter in a preterm neonate. | the authors report a case of bacteremia due to rhizobium radiobacter in a preterm neonate. although the baby recovered from the septic episode following therapy with appropriate antibiotics he succumbed to complications, mainly associated with prematurity. this case highlights a rare manifestation of r.radiobacter infection in a neonate in whom the source of the organism remained undiscovered. | 2014 | 23539251 |
| identification of host proteins modulated by the virulence factor ac2 of tomato chlorotic mottle virus in nicotiana benthamiana. | tomato, one of the most important crops cultivated worldwide, has been severely affected by begomoviruses such as the tomato chlorotic mottle virus (tocmov). virulence factor ac2 is considered crucial for a successful virus-plant interaction and is known to act as a transcriptional activator and in some begomoviruses to function as an rna silencing suppressor factor. however, the exact functions of the ac2 protein of the begomovirus tocmov are not yet established. the aim of the present study wa ... | 2013 | 23533094 |
| yeast competence for exogenous dna uptake: towards understanding its genetic component. | the demonstration of spontaneous yeast competence shows that artificial transformation rests on naturally occurring cellular processes. such natural competence is either biologically mediated or environmentally induced. for instance, wild yeast might be transformed through conjugation by cell-to-cell contact mediated either by escherichia coli or agrobacterium tumefaciens. moreover, natural competence can be enhanced by mechanical and physiological mechanisms. on the other hand, artificial yeast ... | 2013 | 23529158 |
| high frequency regeneration via direct somatic embryogenesis and efficient agrobacterium-mediated genetic transformation of tobacco. | a direct somatic embryogenesis protocol was developed for four cultivars of nicotiana species, by using leaf disc as an explant. direct somatic embryogenesis of nicotiana by using bap and iaa has not been investigated so far. this method does not require formation of callus tissues which leads to somaclonal variations. the frequency of somatic embryogenesis was strongly influenced by the plant growth hormones. the somatic embryos developing directly from explant tissue were noticed after 6 d of ... | 2013 | 23518589 |
| enhancement of heterologous gene expression in flammulina velutipes using polycistronic vectors containing a viral 2a cleavage sequence. | agrobacterium tumefaciens-mediated transformation for edible mushrooms has been previously established. however, the enhancement of heterologous protein production and the expression of multi-target genes remains a challenge. in this study, heterologous protein expression in the enoki mushroom flammulina velutipes was notably enhanced using 2a peptide-mediated cleavage to co-express multiple copies of single gene. the polycistronic expression vectors were constructed by connecting multi copies o ... | 2013 | 23516605 |
| synthesis and structure-activity relationship of n-(cinnamyl) chitosan analogs as antimicrobial agents. | the current study focuses on the preparation of new n-(cinnamyl) chitosan derivatives as antimicrobial agents against nine types of crop-threatening pathogens. chitosan was reacted with a set of aromatic cinnamaldehyde analogs by reductive amination involving formation of the corresponding imines, followed by reduction with sodium borohydride to produce n-(cinnamyl) chitosan derivatives. the structural characterization was confirmed by (1)h and (13)c nmr spectroscopy and the degrees of substitut ... | 2013 | 23511055 |
| transformation of apple (malus × domestica) using mutants of apple acetolactate synthase as a selectable marker and analysis of the t-dna integration sites. | apple acetolactate synthase mutants were generated by site-specific mutagenesis and successfully used as selection marker in tobacco and apple transformation. t-dna/apple genome junctions were analysed using genome-walking pcr and sequencing. an agrobacterium-mediated genetic transformation system was developed for apple (malus × domestica), using mutants of apple acetolactate synthase (als) as a selectable marker. four apple als mutants were generated by site-specific mutagenesis and subsequent ... | 2013 | 23494389 |