Publications

telomere maintenance in human b lymphocytes.	telomere shortening has been causally linked to replicative senescence in human cells. to characterize telomere-length heterogeneity in peripheral blood cells of normal individuals, we analysed the mean length of telomeric repeat sequences in subpopulations of peripheral blood leucocytes, using fluorescence in situ hybridization and flow cytometry (flow-fish). although the telomere length of most haematopoietic subsets was within the same range, the mean telomere length was found to be 15% highe ...	2002	12437664
flow cytometric analysis of procalcitonin expression in human monocytes and granulocytes.	procalcitonin (pct), a precursor of calcitonin is a useful indicator of severe systemic infection and sepsis. for a better understanding of the pathophysiological background of pct induction, a study was made of the intracellular expression of pct in various human white blood cell populations-i.e. monocytes and polymorphonuclear granulocytes (pmns)-and the role of tnf-alpha in the stimulation of their pct production. the expression of pct was investigated by flow cytometric analysis with intrace ...	2002	12413737
murine beta-defensin-3 is an inducible peptide with limited tissue expression and broad-spectrum antimicrobial activity.	beta-defensins are cationic peptides produced by epithelial cells that have been proposed to be an important component of immune function at mucosal surfaces. similarities between mammalian beta-defensins may permit the use of murine models to further define the role of these peptides in innate host defense. murine beta-defensin-3 (mbd-3) is a peptide that exhibits homology at the gene level to human beta-defensin-2 (hbd-2), one of four beta-defensins identified in man. the purpose of this study ...	2002	12412627
[biodistribution of low-molecular-weight proteins and technetium (99mtc) labeled peptides in an animal model of inflammation].	the aim of this study was to evaluate accumulation of the low molecular peptides and proteins labelled with 99mtc in rat inflammatory/infection foci. peptides (human leukocyte dialysate--hld, thymosin fraction 5--tf5, aprotinin--apt), proteins (human igg-hig) were labelled with 99mtc using a redox polymer. the labelling efficiency was evaluated using paper, tlc and/or column chromatography and electrophoresis. the biodistribution of the labelled substances was evaluated in wistar rats with staph ...	2002	12407925
human neutrophil oxidative bursts and their in vitro modulation by different n-acetylcysteine concentrations.	reactive oxygen species released by activated polymorphonuclear leukocytes as an expression of their defensive function are considered to be a major source of the cytotoxic oxidant stress, that triggers a self-sustaining phlogogenic loop in the respiratory system. n-acetylcysteine (cas 616-91-1, nac), a known mucolytic drug, possesses also antioxidant properties, but it undergoes a rapid and extensive first-pass metabolism resulting in low tissue availability. thus to further improve the nac bio ...	2002	12404881
antibiogram and beta-lactamase production of staphylococcus aureus isolates from different human clinical specimens in edo state, nigeria.	the antibiogram and beta-lactamase of 73 isolates of staphylococcus aureus from 235 different human clinical specimens were determined using standard procedures. these various clinical specimens were got from teaching hospitals and some private hospitals in edo state. the results of the antibiogram showed 100% susceptibiity to vancomycin, 78.1% to gentamicin, 71.3% to chloramphenicol, 69.8% to erythromycin and 61.6% to cloxacillin. the results of the beta-lactamase detection showed that 84.1% of ...	2002	12403034
quantity of aerobic bacteria in the bony portion of the external auditory canal of children.	the human external auditory canal (eac) hosts a commensal bacterial flora of mainly non-pathogens, but bacterial pathogens may also be present. the latter are important in the aetiopathogenesis of external otitis and middle ear cholesteatoma with discharge. the purpose of this study was to quantify the bacteria normally harboured in the healthy eac and on a well-defined region of the cavum conchae (cc) of children.	2002	12393252
staphylococcus aureus aconitase inactivation unexpectedly inhibits post-exponential-phase growth and enhances stationary-phase survival.	staphylococcus aureus preferentially catabolizes glucose, generating pyruvate, which is subsequently oxidized to acetate under aerobic growth conditions. catabolite repression of the tricarboxylic acid (tca) cycle results in the accumulation of acetate. tca cycle derepression coincides with exit from the exponential growth phase, the onset of acetate catabolism, and the maximal expression of secreted virulence factors. these data suggest that carbon and energy for post-exponential-phase growth a ...	2002	12379717
[in vitro and in vivo antibacterial activities of pazufloxacin mesilate, a new injectable quinolone].	we investigated the in vitro and in vivo antibacterial activities of pazufloxacin mesilate (pzfx mesilate), a new injectable quinolone, and obtained the following results. 1) the mic50 and mic90 values of pzfx against clinically isolated gram-positive and -negative bacteria, ranged from 0.0125 to 12.5 micrograms/ml and 0.025 to 100 micrograms/ml, respectively. pzfx showed broad spectrum activity. the antibacterial activities of pzfx against quinolone-susceptible, methicillin-resistant staphyloco ...	2002	12378871
chlorpromazine has intracellular killing activity against phagocytosed staphylococcus aureus at clinical concentrations.	chlorpromazine (cpz) has in vitro antimicrobial activity against staphylococcus aureus at concentrations that greatly exceed those achieved clinically. it is concentrated by tissues that are rich in macrophages and it is active against phagocytosed mycobacteria when the concentration in the medium is compatible with that achieved clinically. in this report we show that nontoxic concentrations of cpz below clinical levels have killing activity against s. aureus phagocytosed by human monocyte-deri ...	2002	12373485
enhancement of complement activation and opsonophagocytosis by complexes of mannose-binding lectin with mannose-binding lectin-associated serine protease after binding to staphylococcus aureus.	human mannose-binding lectin (mbl) is a serum protein of the innate immune system that circulates as a complex with a group of so-called mbl-associated serine proteases (masp-1, masp-2, and masp-3). complexes of mbl-masp2 are able to activate the complement system in an ab and c1-independent fashion after binding of the lectin to appropriate microbial sugar arrays. we have evaluated the additive effect of the lectin pathway relative to other complement activation pathways and the subsequent effe ...	2002	12370377
serum concentration of hakata antigen, a member of the ficolins, is linked with inhibition of aerococcus viridans growth.	hakata antigen (hakata) is a novel serum glycoprotein that consists of collagen- and fibrinogen-like domains, similar to ficolin/p35. our research suggested that serum hakata may be a target of a polysaccharide (psa) produced by aerococcus viridans.	2002	12367778
influence of several uropathogenic microorganisms on human sperm motility parameters in vitro.	the effects of certain uropathogenic microorganisms (neisseria gonorrhoeae, staphylococcus aureus, staphylococcus epidermidis and mycobacterium tuberculosis) on human sperm motility characteristics were studied in vitro.	2002	12364972
surfactant wound irrigation for the treatment of staphylococcal clinical isolates.	deep wound infection involving an implanted biomaterial is a devastating complication in orthopaedic surgery. two-thirds of such infections are monomicrobial and the most commonly isolated bacteria in human osteomyelitis and orthopaedic device infection are staphylococcus aureus and staphylococcus epidermidis. the purpose of the current study was to examine the effectiveness of the previously reported sequential surfactant irrigation protocol against human-isolated clinical strains of staphyloco ...	2002	12360010
role of charge properties of bacterial envelope in bactericidal action of human group iia phospholipase a2 against staphylococcus aureus.	mammalian group iia phospholipases a(2) (pla(2)) potently kill staphylococcus aureus. highly cationic properties of these pla(2) are important for ca(2+)-independent binding and cell wall penetration, prerequisites for ca(2+)-dependent degradation of membrane phospholipids and bacterial killing. to further delineate charge properties of the bacterial envelope important in group iia pla(2) action against s. aureus, we examined the effects of mutations that prevent specific modifications of cell w ...	2002	12359734
role of agr (rnaiii) in staphylococcus aureus adherence to fibrinogen, fibronectin, platelets and endothelial cells under static and flow conditions.	in the present study, the adherence of staphylococcus aureus (strain 83254) and its rnaiii mutant (wa400) to immobilised fibrinogen and fibronectin, and to human endothelial cells (ec), was studied. [3h]thymidine-labelled bacteria in stationary phase were incubated on the test surfaces for 20 min under static or flow (200/s) conditions. the results showed: (a) increased adherence of the rnaiii mutant to fibrinogen under static conditions, and decreased adherence of the mutant to fibronectin and ...	2002	12358065
effect of protein binding on the in vitro activity and pharmacodynamics of faropenem.	the influence of protein binding upon different aspects of the in vitro activity of faropenem on recently isolated staphylococcus aureus and respiratory pathogens was determined. the protein binding of faropenem was investigated in inactivated human serum and albumin by ultrafiltration. the effect of the presence of inactivated human serum and albumin on the in vitro activity of faropenem and amoxicillin was established and the influence of protein binding on the pharmacodynamic properties of fa ...	2002	12356797
induction of cytokine production by different staphylococcal strains.	in light of the important role of cytokines in the pathogenesis of bacterial infections, we analyzed the cytokine production induced by different staphylococcus aureus (s. aureus), s. epidermidis and s. saprophyticus strains in human mononuclear cells (mncs). mncs secreted high amounts of interleukin-1beta (il-1beta) and il-6 proteins in responses to stimulation with all three species of staphylococci. interestingly, a large majority of the s. aureus strains induced significantly higher il-12 an ...	2002	12297115
multidrug pump inhibitors uncover remarkable activity of plant antimicrobials.	plant antimicrobials are not used as systemic antibiotics at present. the main reason for this is their low level of activity, especially against gram-negative bacteria. the reported mic is often in the range of 100 to 1,000 micro g/ml, orders of magnitude higher than those of common broad-spectrum antibiotics from bacteria or fungi. major plant pathogens belong to the gram-negative bacteria, which makes the low level of activity of plant antimicrobials against this group of microorganisms puzzl ...	2002	12234835
increased expression of clumping factor and fibronectin-binding proteins by hemb mutants of staphylococcus aureus expressing small colony variant phenotypes.	small colony variants (scvs) of staphylococcus aureus are slow-growing subpopulations that cause persistent and relapsing infections. the altered phenotype of scv can arise from defects in menadione or hemin biosynthesis, which disrupt the electron transport chain and decrease atp concentrations. with scvs, virulence is altered by a decrease in exotoxin production and susceptibility to various antibiotics, allowing their intracellular survival. the expression of bacterial adhesins by scvs is poo ...	2002	12228267
the combination of the sh metabolite of erdosteine (a mucoactive drug) and ciprofloxacin increases the inhibition of bacterial adhesiveness achieved by ciprofloxacin alone.	exposure to ciprofloxacin and other fluoroquinolone antibiotics at less than minimum inhibitory concentrations (mics) reduces the production of some of the factors that contribute to bacterial virulence, particularly bacterial adhesiveness. once metabolized, erdosteine (a mucoactive drug) produces an active metabolite (met i) with a reducing sulfhydryl group that is capable of opening the disulfide bonds present in tracheobronchial mucins and pilins, a protein of bacterial fimbriae (adhesins). t ...	2002	12224380
[antimicrobial, echinococcidial and immunostimulating properties of the drug cheblin-sk-1].	the antimicrobial properties of the drug cheblin-ck-1 (cck-1) were determined in mice intraabdominally inoculated with proteus mirabilis-4691 in a dose of 140-200 million daily cultured microbial bodies. its comparison agent was ampicillin. cck-1 was found to act as an antibiotic similar to ampicillin in its effects. the antimicrobial activity of cck-1 against staphylococcus aureus and escherichia coli isolated from the contents of echinococcal cysts from patients operated on was also establishe ...	2002	12224264
pathology of a mouse model of x-linked chronic granulomatous disease.	a colony of knockout mice (gene designation cybb tm1) has been maintained at this institution for 5 years. these mice are lacking the b subunit of nadph oxidase and are susceptible to experimental infection with aspergillus fumigatus. the purpose of this study was to document the spontaneous diseases present in these mice which are a murine model of x-linked chronic granulomatous disease and to compare these lesions to those of chronic granulomatous disease in humans. lesions were documented in ...	2002	12213046
intravitreal penetration of cefepime after systemic administration to humans.	to investigate the penetration of cefepime (a fourth-generation cephalosporin) into the vitreous after single-dose intravenous administration to human subjects.	2002	12207129
evaluation of the antimicrobial potential of medicinal plants from the ivory coast.	a total of 148 crude ethanol extracts from 115 plant species were tested in vitro against gram-negative strains (escherichia coli, pseudomonas aeruginosa) and the gram-positive staphylococcus aureus and enterococcus faecalis. moreover, they were submitted to antifungal assays against candida albicans and cladosporium cucumerinum, a human and a plant pathogenic microorganism, respectively, known to be good indicators of antifungal activity. no activity was detected against the gram-negative bacte ...	2002	12203276
antibacterial activity in four marine crustacean decapods.	a search for antibacterial activity in different body-parts of pandalus borealis (northern shrimp), pagurus bernhardus (hermit crab), hyas araneus (spider crab) and paralithodes camtschatica (king crab) was conducted. dried samples were extracted with 60% (v/v) acetonitrile, containing 0.1% (v/v) trifluoroacetic acid, and further extracted and concentrated on c18 cartridges. eluates from the solid phase extraction were tested for antibacterial, lysozyme and haemolytic activity. antibacterial act ...	2002	12194450
ocular infections in patients with atopic dermatitis.		2002	12189616
comparative pulsed-field gel electrophoresis typing of gentamicin-resistant and -susceptible methicillin-resistant staphylococcus aureus strains isolated in france between 1991 and 1998. changes in antibiotic susceptibility.	using macrorestriction of genomic dna and pulsed-field gel electrophoresis, we examined 504 non-redundant, infection-causing human isolates of methicillin-resistant staphylococcus aureus susceptible (g(s): 238 isolates) or resistant to gentamicin (g(r): 266 isolates). the strains were isolated at albert chenevier hospital (créteil, france) between 1 january 1991 and 31 december 1998. their susceptibility to erythromycin, lincomycin, tetracycline, rifampicin, fusidic acid and fosfomycin was also ...	2002	12183140
the origin of the synergistic effect of muramyl dipeptide with endotoxin and peptidoglycan.	although the basis for the high mortality rate for patients with mixed bacterial infections is likely to be multifactorial, there is evidence for a synergistic effect of muramyldipeptide (mdp) with lipopolysaccharide (lps) on the synthesis of proinflammatory cytokines by mononuclear phagocytes. in this study, co-incubation of human mono mac 6 cells with mdp and either lps or peptidoglycan (pgn) resulted in an apparent synergistic effect on tumor necrosis factor-alpha (tnf-alpha) secretion. altho ...	2002	12151399
the structure of human macrophage inflammatory protein-3alpha /ccl20. linking antimicrobial and cc chemokine receptor-6-binding activities with human beta-defensins.	human macrophage inflammatory protein-3alpha (mip-3alpha; ccl20) is a cc-type chemokine that binds to and activates cc chemokine receptor-6 (ccr6). although mip-3alpha does not share the binding site of ccr6 with any other chemokine, human beta-defensin-1 and -2, small cationic antimicrobial peptides, have also been found to bind to and activate ccr6. conversely, we have found that mip-3alpha possesses antibacterial activity of greater potency than human beta-defensin-1 and -2 against escherichi ...	2002	12149255
resistance mechanisms of gram-positive bacteria.	the introduction and increasing use of antibiotics for antibacterial therapy has initiated a rapid development and expansion of antibiotic resistance in microorganisms, particularly in human pathogens. additionally, a shift to an increase in number and severity of gram-positive infections has been observed the last decades. common to these pathogens is their tendency to accumulate multiple resistances under antibiotic pressure and selection. methicillin-resistant staphylococcus aureus (mrsa), th ...	2002	12139425
intracellular activity of clinical concentrations of phenothiazines including thioridiazine against phagocytosed staphylococcus aureus.	the effect of thioridazine (tz) was studied on the killing activity of human peripheral blood monocyte derived macrophages (hpbmdm) and of human macrophage cell line thp-1 at extracellular concentrations below those achievable clinically. these macrophages have nominal killing activity against bacteria and therefore, would not influence any activity that the compounds may have against intracellular localised staphylococcus aureus. the results indicated that whereas tz has an in vitro minimum inh ...	2002	12127709
aortic and mitral valve surgery on the beating heart is lowering cardiopulmonary bypass and aortic cross clamp time.	the concept of cardiac surgery on the beating heart is acceptable rationale for the cardiac surgery in the next millenium. beating heart (off-pump) coronary artery bypass grafting (cabg) techniques have led us to consider the possibility for performing the aortic and mitral valve surgery (mitral valve repairs and replacements - with or without cabg) on the beating heart with the technique of retrograde oxygenated coronary sinus perfusion.	2002	12125670
internalization of staphylococcus aureus by human corneal epithelial cells: role of bacterial fibronectin-binding protein and host cell factors.	wild-type staphylococcus aureus was observed to be capable of invading human corneal epithelial cells (hcec) in vitro. internalization of s. aureus required expression of fibronectin-binding proteins (fnbps); the capacity of an fnbp-deficient isogenic strain to invade hcec was reduced by more than 99%. the binding of s. aureus to hcec did not require viable bacteria, since uv-killed cells were observed to adhere efficiently. invasion of hcec by s. aureus involved active host cell mechanisms; upt ...	2002	12117986
bacterium-induced cxcl10 secretion by osteoblasts can be mediated in part through toll-like receptor 4.	two common pathogens known to cause bone infection, salmonella and staphylococcus aureus, were investigated to determine their abilities to induce chemokine expression in cultured mouse and human osteoblasts. while these cells are responsible for bone formation, we were surprised to find that they could respond to bacterial infection by upregulating expression of the chemokine cxcl10 (ip-10). however, there were significant differences in the abilities of the gram-negative bacterium salmonella a ...	2002	12117914
differential role of neutrophil fcgamma receptor iiib (cd16) in phagocytosis, bacterial killing, and responses to immune complexes.	to determine the roles played by the neutrophil fcgamma receptor type ii (fcgammarii) (cd32) and fcgammariiib (cd16) in phagocytosis, bacterial killing, and activation by immune complexes (ics) and to test the hypothesis that inhibition of pathologic effector neutrophil function is possible without compromising host defense.	2002	12115243
activation of human t cells with nk cell markers by staphylococcal enterotoxin a via il-12 but not via il-18.	we have reported recently that mouse liver nk cells and nk1 x 1+ t cells were activated by bacterial superantigens via the il-12 production from kupffer cells. in the present study, we examined the effect of staphyloccoccal enterotoxin a (sea) on human t cells with nk cell markers, cd56 or cd57 (nk-type t cells). after stimulating peripheral blood mononuclear cells (pbmc) with sea, pbmc produced a large amount of ifn- and acquired a potent antitumour cytotoxicity. the in vitro depletion of eithe ...	2002	12109440
typing of human and bovine staphylococcus aureus by rapd-pcr and ribotyping-pcr.	aims: to investigate genetic diversity among staphylococcus aureus and to delineate the geographical distribution of the strains found. methods and results: rapd-pcr and ribotyping-pcr were employed for the characterization of staph. aureus isolates from bovine and nosocomial origin. among the strains, five to nine groups were distinguished by rapd-pcr, depending on which primer was used, while ribotyping-pcr distinguished seven ribotypes. conclusions, and significance and impact of the study: t ...	2002	12081546
molecular characterization of bovine cd26 upregulated by a staphylococcal superantigen.	in this report, we describe the cloning, sequencing, and expression of the bovine orthologue of cd26 (bocd26). several monoclonal antibodies specific for a molecule, activation molecule 3 (act3), aberrantly expressed on superantigen-stimulated bovine cd8(+) lymphocytes, reacted with recombinant bocd26 expressed in cos-7 and cho cells. we also showed that human cd8(+) t cells stimulated by a superantigen expressed cd26 at high levels. these results demonstrate that act3 is identical to bocd26 and ...	2002	12073152
invasion of human keratinocytes by staphylococcus aureus and intracellular bacterial persistence represent haemolysin-independent virulence mechanisms that are followed by features of necrotic and apoptotic keratinocyte cell death.	colonization of human skin by staphylococcus aureus is a characteristic feature of several inflammatory skin diseases, which is often followed by tissue invasion and severe cell damage. a crucial role has been attributed to staphylococcal haemolysins in the cytotoxicity to epidermal structures.	2002	12072060
mimicry of host-defense peptides by unnatural oligomers: antimicrobial beta-peptides.	we have designed beta-amino acid oligomers that are helical, cationic, and amphiphilic with the intention of mimicking the biological activity of amphiphilic, cationic alpha-helical antimicrobial peptides found in nature (e.g., magainins). we have previously identified a 17-residue beta-peptide (called beta-17) with antibiotic activity similar to that of a magainin derivative against four bacterial species, including two clinical isolates that are resistant to common antibiotics. this beta-pepti ...	2002	12071741
genome sequence survey identifies unique sequences and key virulence genes with unusual rates of amino acid substitution in bovine staphylococcus aureus.	staphylococcus aureus is a major cause of mastitis in bovine and other ruminant species. we here present the results of a comparative genomic analysis between a bovine mastitis-associated clone, rf122, and the recently sequenced human-associated clones, mu50 and n315, of staphylococcus aureus. a shotgun sequence survey of approximately 10% of the rf122 genome identified numerous unique sequences and those with elevated rates of nonsynonymous substitution. taken together, these analyses show that ...	2002	12065548
overproduction of type 8 capsular polysaccharide augments staphylococcus aureus virulence.	type 8 capsular polysaccharide (cp8) is the most prevalent capsule type in clinical isolates of staphylococcus aureus. however, its role in virulence has not been clearly defined. cp8 strains such as strain becker produce a small amount of capsule on their surface in vitro. in contrast, cp1 strains such as strain m produce a large amount of capsule, which has been shown to be an important antiphagocytic virulence factor. the cap8 and cap1 operons, required for the synthesis of cp8 and cp1, respe ...	2002	12065477
monocyte deactivation in severe human sepsis or following cardiopulmonary bypass.	we investigated the specificity for gram-negative stimuli as well as the contribution of signal transduction pathways for leukocyte hyporesponsiveness in sepsis or following cardiopulmonary bypass (cpb). whole blood of nine patients undergoing cpb and 25 patients with severe sepsis was stimulated ex vivo with lps (e. coli o111:b4) or with staphylococcus aureus cowan strain i (sac-i) lysate in the absence or presence of inhibitors of protein kinase c (pkc), protein-tyrosine kinase (ptk), or prote ...	2002	12022753
brachyspira (serpulina) pilosicoli of human origin interfere with the haemolytic activity and the growth of staphylococcus aureus beta-toxin producer.	brachyspira (serpulina) pilosicoli related to intestinal spirochaetosis were found to interfere in vitro with the haemolytic activity and the growth of staphylococcus aureus beta-toxin producer. this interference was clearly appreciated because a reduction of the zone of the staphylococcal beta-toxin activity, the reduction and/or absence of cooperative haemolysis between bacteria, and the growth reduction of s. aureus were observed when b. (s.) pilosicoli were grown 72-96 hours sooner than s. a ...	2002	12019720
regulation of human b cell function by sulfasalazine and its metabolites.	although sulfasalazine is a well-known disease-modifying antirheumatic drug (dmard), the mechanisms of its action remain unclear. indeed, it remains uncertain whether sulfasalazine itself or one of its metabolites is responsible for the antirheumatic effects of sulfasalazine. since one of the characteristic features of rheumatoid arthritis (ra) is chronic stimulation of b cells, we compared the effects of sulfasalazine and its metabolites on the in vitro function of human b cells. ig production ...	2002	12013503
induction of cytokine production in human t cells and monocytes by highly purified lipoteichoic acid: involvement of toll-like receptors and cd14.	the pro-inflammatory potential of lipoteichoic acid (lta) from staphylococcus aureus is controversial. the present study was undertaken to examine the ability of highly purified and characterized s. aureus lta to stimulate the production of pro-inflammatory cytokines in human leukocytes at both mrna and protein level, and to study the involvement of toll-like receptors (tlrs) and cd14 in this response.	2002	12011760
new aspects of the tolerance of the antiseptic povidone-iodine in different ex vivo models.	investigating new possibilities for the application of 1% (v/v) iodophors, povidone-iodine (pvp-i) was better tolerated in the het-cam or explant test than 1% (w/v) silver nitrate or tetracycline. after application to the eye, at least 2.6% of used iodine were adsorbed. therefore pvp-i is more effective than silver nitrate or erythromycin, meaning a possible alternative for the prevention of ophthalmia neonatorum. pvp-i is more active against methicillin-resistant staphylococcus aureus (mrsa) in ...	2002	12011528
mitral valve endocarditis in hypertrophic cardiomyopathy: case report and literature review.	mitral endocarditis complicating hypertrophic cardiomyopathy occurs predominantly on the left ventricular aspect of the anterior mitral valve leaflet in the presence of outflow tract obstruction. it is a rare condition and the estimated cumulative 10 year probability of developing endocarditis in patients with obstruction is < 5%. combined mitral valve replacement and septal myectomy has been reported in this setting. a case of community acquired staphylococcus aureus mitral valve endocarditis i ...	2002	12010956
identification of in vivo expressed vaccine candidate antigens from staphylococcus aureus.	for the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. we have developed a rapid and efficient procedure to identify such commonly recognized antigens, and here we provide a comprehensive in vivo antigenic profile of staphylococcus aureus, an important human pathogen. s. aureus peptides were displayed on the surface of escherichia coli via fusion to one of two outer membrane pr ...	2002	11997460
the extracellular toll-like receptor 2 domain directly binds peptidoglycan derived from staphylococcus aureus.	toll-like receptor 2 (tlr2) has been recognized to mediate cell signaling in response to peptidoglycan (pgn), a major cell wall component of gram-positive bacteria. the mechanism by which tlr2 recognizes pgn is unknown. it is not even clear whether tlr2 directly binds to pgn. in this study, we generated a soluble form of recombinant tlr2 (stlr2) possessing only its putative extracellular domain by using the baculovirus expression system to examine the direct interaction between stlr2 and pgn. st ...	2002	11986301
antibacterial activities and conformations of synthetic alpha-defensin hnp-1 and analogs with one, two and three disulfide bridges.	structure and biological activities of synthetic peptides corresponding to human alpha-defensin hnp-1, ac1yc2ripac3iagerrygtc4iyqgrlwafc5c6 with the s-s connectivities: c1-c6, c2-c4, c3-c5, and its variants with one, two and three disulfide bridges were investigated. oxidation of synthetic, reduced hnp-1 yielded a peptide with s-s connectivities c1-c3, c2-c4 and c5-c6, and not with the s-s linkages as in naturally occurring hnp-1. selective protection of cysteine sulfhydryls was necessary for th ...	2002	11985703
antibody reactivity of a standardized human serum protein solution against a spectrum of microbial pathogens and toxins: comparison with fresh frozen plasma.	in this study, we compared a standardized solution of human serum protein (hsp) and fresh frozen plasma (ffp) with regard to the antibody specificity against a number of microbial pathogens and some important pathogenicity factors of bacterial pathogens. due to the clinical use of hsp and ffp for therapeutical plasma exchange, we have chosen a spectrum of microbial pathogens for serological analysis that is critical in clinical settings. with the enzyme-linked immunosorbent assay technique, we c ...	2002	11982956
staphylococcal exfoliative toxin b specifically cleaves desmoglein 1.	staphylococcal scalded skin syndrome and its localized form, bullous impetigo, show superficial epidermal blister formation caused by exfoliative toxin a or b produced by staphylococcus aureus. recently we have demonstrated that exfoliative toxin a specifically cleaves desmoglein 1, a desmosomal adhesion molecule, that when inactivated results in blisters. in this study we determine the target molecule for exfoliative toxin b. exfoliative toxin b injected in neonatal mice caused superficial epid ...	2002	11982763
corneal expression of the inflammatory mediator cap37.	cap37 is a polymorphonuclear neutrophil (pmn)-derived inflammatory protein with potent antibiotic and chemotactic activity. to further investigate the biological significance of cap37 in infection and inflammation, a well-characterized in vivo rabbit model of bacterial keratitis was selected to study its contribution to host defenses.	2002	11980855
staphylococcal enterotoxin h contrasts closely related enterotoxins in species reactivity.	staphylococcus aureus enterotoxin h (seh) belongs to the staphylococcal enterotoxin (se) family of superantigens (sags). seh has structural similarities to other se; however, its biological properties are less well characterized. seh binds with high affinity to human major histocompatibility complex (mhc) class ii and exhibits strong mitogenic activity in human t cells, although it was found to be less potent than the related sea. surprisingly and in sharp contrast to related ses, seh did not po ...	2002	11972634
profound differences in leukocyte-endothelial cell responses to lipopolysaccharide versus lipoteichoic acid.	we have investigated the effects of lps from escherichia coli, lipoteichoic acid (lta), and peptidoglycan (pepg) from staphylococcus aureus, and live s. aureus on leukocyte-endothelial interactions in vivo using intravital microscopy to visualize muscle microvasculature. systemic vs local administration of lps induced very different responses. local administration of lps into muscle induced significant leukocyte rolling, adhesion, and emigration in postcapillary venules at the site of injection. ...	2002	11971014
different aminoglycoside-resistant phenotypes in a rabbit staphylococcus aureus endocarditis infection model.	the impact of different types of enzymatic resistance on the in vivo antibacterial activity of aminoglycosides (amikacin, gentamicin, and netilmicin) was studied in the rabbit endocarditis model with four strains of staphylococcus aureus. animals were treated in a manner simulating the administration of a single daily human dose. amikacin had no effect on the three kanamycin-resistant strains despite apparent susceptibility in the disk diffusion test. gentamicin appears to be the preferable amin ...	2002	11959609
transferrin binding in staphylococcus aureus: involvement of a cell wall-anchored protein.	the ability to gain access to iron is pivotal for bacterial pathogens during infection. although much is known about iron acquisition systems in gram-negative bacteria, comparatively little is known about how gram-positive pathogens access iron from host iron sources. a previous study showed that, in the gram-positive human pathogen staphylococcus aureus, a cell surface-associated glyceraldehyde-3-phosphate dehydrogenase (gapdh) enzyme (gap, or tpn) is capable of binding human transferrin, repre ...	2002	11952908
tailoring an antibacterial peptide of human lysosomal cathepsin g to enhance its broad-spectrum action against antibiotic-resistant bacterial pathogens.	neutrophils contain several cationic antimicrobial proteins or peptides (caps) that exert antibiotic-like action against bacteria. these host-derived antibiotics kill susceptible bacteria by oxygen-independent mechanisms. considerable interest in their activity has been generated in recent years due not only to their likely important role in innate host defense against infection, but also their possible use as therapeutic agents in treating infections caused by antibiotic-resistant pathogens. we ...	2002	11945165
in vitro interactions of biomedical polyurethanes with macrophages and bacterial cells.	three commercial medical-grade polyurethanes (pus), a poly-ether-urethane (pellethane), and two poly-carbonate-urethanes, the one aromatic (bionate) and the other aliphatic (chronoflex), were tested for macrophages and bacterial cells adhesion, in the presence or absence of adhesive plasma proteins. all the experiments were carried out on pus films obtained by solvent casting. the wettability of these films was analysed by measuring static contact angles against water. the ability of the selecte ...	2002	11939455
function of the 90-loop (thr90-glu100) region of staphylokinase in plasminogen activation probed through site-directed mutagenesis and loop deletion.	staphylokinsae (sak) forms a bimolecular complex with human plasmin(ogen) and changes its substrate specificity by exposing new exosites that enhances accession of substrate plasminogen (pg) to the plasmin (pm) active site. protein modelling studies indicated the crucial role of a loop in sak (sak 90-loop; thr(90)-glu(100)) for the docking of the substrate pg to the sak-pm complex. function of sak 90-loop was studied by site-directed mutagenesis and loop deletion. deletion of nine amino acid res ...	2002	11936951
distribution of staphylococcal enterotoxin genes among staphylococcus aureus isolates from poultry and humans with invasive staphylococcal disease.	food poisoning by staphylococcus aureus affects hundreds of thousands of people each year. staphylococcus aureus also causes invasive diseases such as arthritis (in poultry) and septicemia (in poultry and humans). foodborne disease is caused by the ingestion of a staphylococcal enterotoxin (se). enterotoxin has also been associated with other s. aureus illnesses in humans and domestic animals. in this study, polymerase chain reaction was used to detect the staphylococcal enterotoxin genes, sea, ...	2002	11922324
characterization of staphylococcus aureus isolated from poultry meat in spain.	ninety-six staphylococcus aureus isolates from retail chicken carcasses in spain were characterized using cultural and biochemical tests. the strains were phage typed with the international bacteriophage set for typing s. aureus of human origin. eighty-eight (91.7%) strains were of the poultry ecovar. strains of human ecovar were not found. these facts are congruent with findings of other authors. ninety (93.7%) strains were phage typeable. lysis by phages of group iii was the most frequent with ...	2002	11902420
bacterial pneumonia. managing a deadly complication of influenza in older adults with comorbid disease.	in patients with influenza, the risk of death from pneumonia is closely associated with age and chronic conditions. mortality from influenza and pneumonia in americans age > or = 65 has been increasing since 1980. pneumonia following influenza is usually caused by a secondary bacterial infection. pathogens most commonly implicated are streptococcus pneumoniae, staphylococcus aureus, and haemophilus influenzae. prompt empiric therapy effective against the suspected pathogen is indicated, whether ...	2002	11899549
identification of a transcytosis epitope on staphylococcal enterotoxins.	staphylococcal enterotoxins (se) are exoproteins produced by staphylococcus aureus that act as superantigens and have been implicated as a leading cause of food-borne disease and toxic shock. little is known about how these molecules penetrate the gut lining and gain access to both local and systemic immune tissues. to model movement in vitro of staphylococcal enterotoxins, we have employed a monolayer system composed of crypt-like human colonic t-84 cells. seb and sea showed comparable dose-dep ...	2002	11895985
staphylococcus aureus and escherichia coli adhesion to human cells is reduced by sub-mics of gemifloxacin.	this study was designed to investigate the capacity of subinhibitory concentrations of the newly developed fluoroquinolone antibiotic gemifloxacin to interfere with the mechanism of bacterial adhesion. human buccal epithelial cells were incubated with staphylococcus aureus and escherichia coli, and grown in the presence of serial dilutions of gemifloxacin from 1/2 mic to 1/128 mic. a significant decrease in the adhesion of both s. aureus and e. coli was observed from 1/2 mic to 1/32 mic. morphol ...	2002	11892898
pulmonary infections in patients with cystic fibrosis.	cystic fibrosis (cf) is an autosomal-recessive disorder and affects about 60,000 people worldwide. the cf gene, the cystic fibrosis transmembrane conductance regulator (cftr), was found in 1989 and over 800 mutations have been sequenced. although our understanding of the pathophysiology of cf has increased, pulmonary infections remain the major cause of morbidity and mortality. during the first decade of life, staphylococcus aureus and nontypable haemophilus influenza are most common, but pseudo ...	2002	11891518
rab5a gtpase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils.	biogenesis of phagolysosomes proceeds through a sequential series of interactions with endocytic organelles, a process known to be regulated by rab and snare proteins. the molecular mechanisms underlying phagosome maturation in neutrophils are, however, not clearly understood. we investigated fusion between phagosomes containing the intracellular pathogen mycobacterium tuberculosis versus the extracellular pathogen staphylococcus aureus (designated mcp for mycobacteria-containing phagosome and s ...	2002	11884531
a study on staphylococcus aureus strains submitted to a reference laboratory.	staphylococcus aureus is regarded as one of the most devastating human pathogens. recently there have been reports of increasing incidence of s. aureus strains resistant to methicillin (mrsa). a surveillance study was undertaken to record the occurrence of mrsa and to study the prevalence of various phage groups in india.	2001	11873403
cq-397 and cq-414: antimicrobial activity and spectrum of two fluoroquinolone---cephalosporin, dual-action compounds with carboxamido bonds.	objective: to evaluate the potential spectrum of activity of two novel dual-action compounds with carboxamido bonds (cq-397 and cq-414; laboratorios aranda, san rafael, mexico) against human pathogens. methods: approximately 800 gram-positive and gram-negative aerobic clinical bacteria were tested in vitro using the mueller-hinton broth microdilution method of the national committee of clinical laboratory standards. results: cq-397 (cefamandole+enrofloxacin) and cq-414 (cefamandole+norfloxacin) ...	1997	11864130
retrieving biological activity from lukf-pv mutants combined with different s components implies compatibility between the stem domains of these staphylococcal bicomponent leucotoxins.	bicomponent leucotoxins, such as panton-valentine leucocidin, are composed of two classes of proteins, a class s protein such as luks-pv, which bears the cell membrane binding function, and a class f protein such as lukf-pv, which interacts to form a bipartite hexameric pore. these leucotoxins induce cell activation, linked to a ca(2+) influx, and pore formation as two consecutive and independently inhibitable events. knowledge of the lukf-pv monomer structure has indicated that the stem domain ...	2002	11854215
antibacterial properties of dermaseptin s4 derivatives with in vivo activity.	derivatives of the cytotoxic peptide dermaseptin s4 have recently emerged as potential antimicrobial agents. here, we report on the antibacterial properties of three derivatives with improved toxicity profiles: a 28-residues k4k20-s4 and two shorter versions, k4-s4(1-16) and k4-s4(1-13). the range of mics of k4k20-s4 against clinical isolates of staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli were, respectively, 1 to 4, 1 to 4, and 1 to 16 microg/ml. mics of the short derivat ...	2002	11850249
definition of a type of abnormal vaginal flora that is distinct from bacterial vaginosis: aerobic vaginitis.	to define an entity of abnormal vaginal flora: aerobic vaginitis.	2002	11845812
human beta-defensin-2 production in keratinocytes is regulated by interleukin-1, bacteria, and the state of differentiation.	intact human epidermis resists invasion by pathogenic microbes but the biochemical basis of its resistance is not well understood. recently, an antimicrobial peptide, human beta-defensin-2, was discovered in inflamed epidermis. we used a recombinant baculovirus/insect cell system to produce human beta-defensin-2 and confirmed that at micromolar concentrations it has a broad spectrum of antimicrobial activity, with the striking exception of staphylococcus aureus. immunostaining with a polyclonal ...	2002	11841544
staphylococcal alpha-toxin provokes neutrophil-dependent cardiac dysfunction: role of icam-1 and cys-leukotrienes.	the role of polymorphonuclear neutrophils (pmn) in septic myocardial dysfunction is presently unknown. staphylococcus aureus infections are frequently associated with septic sequelae. therefore, we perfused isolated rat hearts with low doses of alpha-toxin, the major staphylococcal exotoxin, followed by application of human pmn, n-formyl-methionyl-leucyl-phenylalanine, and arachidonic acid. in contrast to sham-perfused hearts (no alpha-toxin), a rise in coronary perfusion pressure (cpp) and a re ...	2002	11834515
the effects of charge and lipophilicity on the antibacterial activity of undecapeptides derived from bovine lactoferricin.	we have investigated the effects of charge and lipophilicity on the antibacterial activity of an undecapeptide (fkcrrwqwrmk) derived from the sequence of bovine lactoferricin. we prepared ten analogues that were modified by the incorporation of ala, tyr, trp, met and arg residues, which are amino acids known to be important for the antibacterial activity of longer derivatives of lactoferricins. all undecapeptides contained the native trp residues in positions 6 and 8, and the arg residues in pos ...	2002	11831560
[the effect of lactate-buffered peritoneal dialysis fluid in human peritoneal mesothelial cells function].	to investigate the effect of lactate-buffered peritoneal dialysis fluid (l-pdf) on human peritoneal mesothelial cells (hpmc) function.	2001	11798616
relationships between staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease.	the expression of most staphylococcus aureus virulence factors is controlled by the agr locus, which encodes a two-component signaling pathway whose activating ligand is an agr-encoded autoinducing peptide (aip). a polymorphism in the amino acid sequence of the aip and of its corresponding receptor divides s. aureus strains into four major groups. within a given group, each strain produces a peptide that can activate the agr response in the other member strains, whereas the aips belonging to dif ...	2002	11796592
fibronectin-binding proteins of staphylococcus aureus are involved in adherence to human airway epithelium.	this study was designed to investigate the molecular mechanisms of staphylococcus aureus adherence to human airway epithelium. using a humanized bronchial xenograft model in the nude mouse and primary cultures of human airway epithelial cells (haec), we showed that s. aureus adhered mainly to undifferentiated haec whereas weak adherence (11- to 20-fold lower) to differentiated haec was observed (p < 0.01). a fibronectin (fn)-binding protein (fnbp)-deficient strain of s. aureus had a fivefold-low ...	2002	11796591
discrepancy between uptake and intracellular activity of moxifloxacin in a staphylococcus aureus-human thp-1 monocytic cell model.	the correlation between uptake of moxifloxacin by thp-1, a continuous line of monocytic cells devoid of intrinsic bactericidal properties, and its activity against staphylococcus aureus atcc 25923, a susceptible reference strain (mic and minimal bactericidal concentration of moxifloxacin, 0.1 mg/liter), was studied in a 5-h assay. the uptake of the drug, added to the culture medium at 0.2 to 32 mg/liter, was evaluated by high-performance liquid chromatography. the ratio of the cellular to extrac ...	2002	11796332
staphylococcus aureus alpha toxin mediates polymorphonuclear leukocyte-induced vasocontraction and endothelial dysfunction.	the effect of staphylococcus aureus alpha toxin (alpha-toxin) on selectin-mediated neutrophil adhesion was investigated in polymorphonuclear leukocyte- (pmn) induced vasocontraction and endothelial dysfunction. adherence of human pmns to rat aortic endothelium increased significantly following stimulation of the endothelium with alpha-toxin (0.1, 0.5, and 1 microg/ml). this effect could be significantly attenuated by monoclonal antibodies directed against p-selectin or fucoidin, a carbohydrate k ...	2002	11795666
the different process of class switching and somatic hypermutation; a novel analysis by cd27(-) naive b cells.	the relationship between class switch recombination (csr) and somatic hypermutation has been unclear. by using human cd27(-) naive b cells, we investigated the somatic hypermutation and producibility of immunoglobulins (igs) that occur after csr. although neither adult cd27(-) nor cord blood b cells, which showed the unmutated ig v-region genes, produced igg, igm, or iga in response to conventional stimuli, they produced igg and igm but not iga in the presence of staphylococcus aureus cowan stra ...	2002	11781240
tissue expression of human toll-like receptors and differential regulation of toll-like receptor mrnas in leukocytes in response to microbes, their products, and cytokines.	members of the toll-like receptor (tlr) family mediate dorsoventral patterning and cellular adhesion in insects as well as immune responses to microbial products in both insects and mammals. tlrs are characterized by extracellular leucine-rich repeat domains and an intracellular signaling domain that shares homology with cytoplasmic sequences of the mammalian il-1 receptor and plant disease resistance genes. ten human tlrs have been cloned as well as rp105, a protein similar to tlr4 but lacking ...	2002	11777946
impaired gp-91phox gene expression and dysfunction of peripheral blood neutrophils in patients maintaining hemodialysis.	to investigate expressions of gp-91phox and il-8 gene and the function of neutrophils in hemodialysis (hd) patients, and to understand the relationship between uremia and immune.	2000	11775533
molecular cloning and biochemical characterisation of proteases from staphylococcus epidermidis.	we report the complete coding sequence and the partial amino acid sequence (determined by chemical sequencing) of staphylococcus epidermidis extracellular cysteine (ecp) and serine (esp) proteases. the first enzyme shows an extended sequence similarity to staphylococcus aureus cysteine protease (staphopain) and the second one resembles the serine protease produced by that species. the region directly upstream of the sequence coding for the mature protein in both enzymes displays significant homo ...	2001	11767947
lactoferrin, amylase and mucin muc5b and their relation to the oral microflora in hyposalivation of different origins.	there are several reasons for hyposalivation, each affecting the salivary composition in different ways. the aim of this study was to analyze and compare lactoferrin, amylase and mucin muc5b in stimulated whole saliva collected from subjects with hyposalivation of different origins and to relate the results to the presence of some microbial species associated with oral disorders. albumin was determined as a marker of serum leakage. the characteristic feature for subjects with radiation-induced h ...	2001	11737657
sequence of the 50-kb conjugative multiresistance plasmid pre25 from enterococcus faecalis re25.	the complete 50,237-bp dna sequence of the conjugative and mobilizing multiresistance plasmid pre25 from enterococcus faecalis re25 was determined. the plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. chloramphenicol acetyltransferase and the 23s rna methylase are identical to gene products of the broad-host-range plasmid pip501 from streptococcus agalactiae. in addition, a 30.5-kb segment is almost identical to pip501. genes encoding an aminoglycos ...	2001	11735367
attachment of staphylococcus aureus to eukaryotic cells and experimental pitfalls in staphylococcal adherence assays: a critical appraisal.	staphylococcus aureus is a bacterial species with pathogenic potential to both humans and animals. the primary natural niche is said to be the human vestibulum nasi from where bacterial cells may spread to the environment or additional anatomical sites such as the perineum or the hands, where residence is usually transient. apparently, s. aureus is capable of a precise and balanced interaction with specific types of eukaryotic nasal cells. although a wide variety of important bacterial ligands a ...	2002	11733080
[drug-induced pneumonitis caused by sulfamethoxazole-trimethoprim].	a 33-year-old man was treated with sulfamethoxazole-trimethoprim (smt-tmp) for an infection in the cervical vertebrae by methicillin-resistant staphylococcus aureus (mrsa). two weeks later a fever of 39 degrees c appeared, and a productive cough, hemosputum, and dyspnea developed a further three weeks later. chest radiographs showed bilateral ground-glass opacity. cell differentiation of bronchoalveolar lavage fluid (balf) revealed increases of lymphocytes and eosinophils, and the cd4/cd8 ratio ...	2001	11729685
methicillin-resistant staphylococcus aureus sepsis associated with the transfusion of contaminated platelets: a case report.	platelet transfusion-associated sepsis is usually due to donor skin flora introduced into the unit during phlebotomy. an unusual case of a platelet component contaminated with methicillin-resistant staphylococcus aureus (mrsa) is reported.	2001	11724990
enterotoxigenic potential of staphylococcus intermedius.	staphylococcal food poisoning (sfp) caused by enterotoxigenic staphylococci is one of the main food-borne diseases. in contrast to staphylococcus aureus, a systematic screening for the enterotoxins has not yet been performed on the genomic level for the coagulase-positive species s. intermedius. therefore, the enterotoxigenic potential of 281 different veterinary (canine, n = 247; equine, n = 23; feline, n = 9; other, n = 2) and 11 human isolates of s. intermedius was tested by using a multiplex ...	2001	11722906
treatment of acrodermatitis continua of hallopeau with oral propylthiouracil and methotrexate.	a 71-year-old man with acrodermatitis continua of hallopeau was treated successfully with a combination of oral propylthiouracil and methotrexate. after 14 weeks, he developed acute pancytopenia, an uncommon idiosyncratic side-effect of propylthiouracil, and presented with a life-threatening methicillin-resistant staphylococcus aureus pneumonia. this illustrates the potential value and associated risks of propylthiouracil in the management of this difficult condition.	2001	11722449
hematogenous cervical spondylodiscitis after severe burn injury.	a 47-year-old man sustained a 31% tbsa burn injury. in spite of early escharectomy and mesh-graft-transplantation the patient suffered a septicaemic phase in the first week, which was treated by a specific antibiotic. five weeks after the burn injury a cervical spondylodiscitis was diagnosed. immediate wound debridement, ventral and dorsal spondylodesis with a tricortical bone-graft from the left iliac crest and titanium plates and specific antibiotic therapy led to the stabilization and healing ...	2001	11718988
environmental and body contamination through aerosols produced by high-speed cutters in lumbar spine surgery.	a cadaver study to evaluate contamination in the operating room through the use of a high-speed bone cutter.	2001	11698896
antibacterial effect of human v gamma 2v delta 2 t cells in vivo.	v gamma 2v delta 2 cells, a class of t cells found only in primates, are reactive to nonpeptide organophosphate and alkylamine antigens secreted by bacteria and parasites. these cells make up 2-5% percent of human peripheral blood t cells but expand to make up 8-60% of peripheral blood t cells during bacterial and parasitic infections. we show here, using a chimeric severe combined immunodeficiency (scid) mouse (hu-scid) model, that human v gamma 2v delta 2 t cells mediate resistance to extracel ...	2001	11696580
multiplex pcr for simultaneous identification of staphylococcus aureus and detection of methicillin and mupirocin resistance.	in this work, we describe a multiplex pcr assay for the detection of clinically relevant antibiotic resistance genes harbored by some staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. conditions were optimized for the simultaneous detection of the 310-, 456-, and 651-bp regions of the meca (encoding high-level methicillin resistance), iles-2 (encoding high-level mupirocin resistance), and femb (encoding a factor essential for methicilli ...	2001	11682527
synthesis and biological evaluation of azole derivatives, analogues of bifonazole, with a phenylisoxazolyl or phenylpyrimidinyl moiety.	a series of azole derivatives, isoxazole or pyrimidine analogues of the antifungal drug bifonazole, were synthesized and tested in vitro against representative human pathogenic fungi (candida albicans, cryptococcus neoformans and aspergillus fumigatus). they were also evaluated as antibacterial agents against staphylococcus aureus and salmonella spp. only 5-(imidazol-1-yl-phenylmethyl)-2,4-diphenyl-pyrimidine 7c showed weak antimicrobial activity (mic = 66 microm) against c. albicans, c. neoform ...	2001	11680806
dissecting folliculitis of the scalp with marginal keratitis.	we describe a young man with dissecting folliculitis of the scalp, who subsequently developed marginal keratitis. we are unaware of any previous reports of this association. the aetiology of dissecting folliculitis is thought to relate to abnormal follicular occlusion with subsequent suppuration, although an infective process has never been established. marginal keratitis is postulated, but not proven, to be caused by an enhanced immune response to staphylococcus aureus antigens. we suggest that ...	2001	11678871
synthesis and antibacterial study of unsaturated mannich ketones.	several mannich ketones of 2-arylmethylenecycloalkanones were synthesised using the classical acid-catalysed mannich reaction. antibacterial activity of these new water-soluble compounds was reported against pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, staphylococcus saprophyticus, micrococcus luteus and bacillus subtilis standard strains. human cell line cytotoxicity of our new compounds was evaluated against hela cell lines. some compounds showed low cytotoxicity (41.52 nm ...	2001	11672880