Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bicp22 of bovine herpesvirus 1 is encoded by a spliced 1.7 kb rna which exhibits immediate early and late transcription kinetics. | kinetic analysis of the two divergent immediate early (ie) transcription units of bovine herpesvirus 1 (bhv-1) revealed an unexpected behaviour. the ie1.7 promoter was not turned off at the end of the ie period but acted as a late promoter, unlike the adjacent ie4.2/2.9 promoter which was active only under ie conditions. the genome region specifying the ie1.7 gene was sequenced (0.814 to 0.839 map units). the ie1.7 promoter was found to overlap with duplicated sequence elements bearing close sim ... | 1994 | 8021599 |
| studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents. | the application of three photosensitive agents for disinfection of bovine semen was investigated. bovine microbial pathogens suspended in tissue culture medium and/or pbs and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, mycoplasma bovigenitalium, mycoplasma canadense, and ureaplasma diversum in culture media. in ... | 1994 | 8017031 |
| effects of supplemental chromium on antibody responses of newly weaned feedlot calves to immunization with infectious bovine rhinotracheitis and parainfluenza 3 virus. | the objective of this study was to determine the effect of supplemental dietary chromium (cr) on antibody responses of feedlot calves. fifty-five newly weaned calves were divided into two groups, 28 that received supplemental cr and 27 that did not, and were immunized with a commercial vaccine against bovine infectious rhinotracheitis virus (ibr) and bovine parainfluenza virus type 3(pi-3). sera harvested from blood sampled preimmunization, and at days 14 and 28 postimmunization (pi), were assay ... | 1994 | 8004541 |
| bovine x murine t-cell hybridomas specific for bovine herpesvirus 1 (bhv-1) glycoproteins. | difficulties in the isolation and long-term maintenance of bovine herpesvirus-1 (bhv-1) specific t-cell clones have hindered the analysis of bovine cell-mediated immune response to this virus. in an effort to identify the t-cell epitopes of the virus, bovine murine t-cell hybridomas specific for bhv-1 were generated as an alternative to t-cell clones. peripheral blood lymphocytes from a calf immunized with bhv-1 were restimulated in vitro with the virus to generate bulk t-cell cultures. the anti ... | 1994 | 7986332 |
| human lymphoblastoid interferon as vaccine adjuvant in cattle. | human lymphoblastoid interferon from namalwa cells was purified for clinical use by ethanol fractionation, and used as adjuvant of an inactivated bovid herpesvirus 1 vaccine in calves. in agreement with other in vitro and in vivo models, low and high interferon doses were shown to increase and depress the specific antibody response, respectively. the low, effective interferon dose (100 international units/kg) also reduced the variability of antibody titres after the first vaccine injection. this ... | 1994 | 7976493 |
| identification of the bovine herpesvirus 1 circ protein, a myristylated and virion-associated polypeptide which is not essential for virus replication in cell culture. | we have recently reported immediate-early (ie) transcription over covalently joined genome ends of bovine herpesvirus 1 (bhv-1). a spliced 1.5-kb ie rna (ier1.5) is coterminal with an unspliced 1.1-kb late rna (lr1.1) which is transcribed from the left end of the genome. sequence analysis reveals an open reading frame common to ier1.5 and lr1.1 predicted to encode the 247-amino-acid circ polypeptide. this paper reports on the identification of circ as a protein. using a rabbit antiserum raised a ... | 1994 | 7966598 |
| mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the ge gene from hsv-1. | in order to map and identify the glycoprotein-encoding gene from bovine herpesvirus type 1 (bhv-1), homologous to the ge glycoprotein from herpes simplex virus type 1 (hsv-1), a region of the unique short sequence from the bhv-1 genome has been sequenced. the sequenced region contains an orf coding for a polypeptide of 575 amino acids (aa). the aa sequence presents substantial similarity to that of the glycoprotein ge from hsv-1 and to homologous proteins of related viruses such as pseudorabies ... | 1994 | 7958994 |
| selection of european union standard reference sera for use in the serological diagnosis of infectious bovine rhinotracheitis. | a study, consisting of three trials, was undertaken in order to find standard reference sera for use in serological diagnostic procedures for infectious bovine rhinotracheitis (ibr) in the european union (eu). a total of forty-nine laboratories participated in the trials, in which panels of positive, weak positive, and negative candidate sera were tested blind by neutralisation, indirect and blocking enzyme-linked immunosorbent assay, indirect immunofluorescence and passive haemagglutination. a ... | 1994 | 7949366 |
| strong transcriptional activators isolated from viral dna by the 'activator trap', a novel selection system in mammalian cells. | transcription factors often contain activation domains that interact with the basic transcription machinery. we have developed a functional screening strategy in mammalian cells to selectively isolate activation domains from a library of random dna inserts. for this, sonicated dna fragments are cloned next to the dna binding domain of gal4 factor in a plasmid that also contains the sv40 origin of replication. pools of fusion protein clones are transfected into cv-1-5gt monkey cells containing an ... | 1994 | 7937127 |
| induction of mhc-unrestricted cytolytic cd4+ t cells against virally infected target cells by cross-linking cd4 molecules. | cross-linking cd4 molecules with the use of specific mabs induced ag-nonspecific, mhc-unrestricted killing by human or bovine cd4+ t cells and human allospecific t cell clones against virally infected (bovine herpesvirus-1 and herpes simplex virus-1) target cells. heat-aggregated goat igg anti-mouse igg elevated cd4 cross-linking efficiency, resulting in augmented killing activity by effector cells. however, ab-activated effector cells failed to kill tumor target cell lines, e.g., k562, daudi (h ... | 1994 | 7930600 |
| variability occurs in the inverted repeat region of genomic dna from bovine herpesvirus 1 respiratory, genital and bovine herpesvirus 5 encephalitic isolates. | restriction fragment length polymorphisms (rflps) were detected within bhv1.1, bhv1.2, and bhv5 genomes using the restriction enzyme psti. the genomic areas of these changes has not been previously reported. using southern blot hybridization with dna probes representing the entire genome of bhv1.1, areas of genomic variation were located for a respiratory isolate (bhv1.1), four vaccine isolates (bhv1.1), a genital isolate (bhv1.2), and two encephalitic isolates (bhv5). the most frequently observ ... | 1993 | 7907451 |
| madin darby bovine kidney cell synchronization by lovastatin: application to bovine herpesvirus-1 gene expression. | the number of investigations involving cell proliferation has increased rapidly in the last years. one of the major difficulties in studying cell-cycle-related events is obtaining highly synchronous cell populations without metabolic imbalance. this study demonstrates that the madin darby bovine kidney (mdbk) cells, a commonly used cell line in veterinary research, can be effectively synchronized using lovastatin (lov), a drug used to treat hypercholesteremia in humans. this was demonstrated by ... | 1994 | 7889037 |
| delineation of the essential function of bovine herpesvirus 1 gd: an indication for the modulatory role of gd in virus entry. | the entry process of alphaherpesviruses consists of two steps, initial virus attachment and subsequent virus penetration involving membrane fusion. glycoprotein d (gd) of the alphaherpesvirus bovine herpesvirus 1 (bhv 1) is an essential envelope protein, and it has been previously documented that gd plays a significant part in both of the virus entry steps. in order to gain further insight into the virus entry process, we attempted to define the essential function of bhv 1 gd. we replaced the gd ... | 1995 | 7886947 |
| a congenital abnormality of calves, suggestive of a new type of arthropod-borne virus infection. | about 1000 calves with a congenital disease were born in kagoshima prefecture, japan, between october 1990 and october 1991, the peaks of the epidemic being in march and july 1991. of 85 abnormal calves examined pathologically and serologically, 70 appeared to have been suffering from a viral disease. of these 70 animals, 17 had lesions bearing some resemblance to those of the diseases produced by akabane, chuzan, aino, bluetongue and bovine viral diarrhoea-mucosal disease viruses-diseases known ... | 1994 | 7884059 |
| double blind field evaluation of a trivalent vaccine against respiratory disease in veal calves. | a field trial was performed to determine the effect of a trivalent vaccine on clinical respiratory tract problems (crtp) in veal calves. the vaccine has been developed to increase immunity against the causal agents of ibr, bvd and brsv infection. in total 928 calves, housed in 16 compartments of one herd, were involved. in four compartments of 58 calves each, vaccinated and non-vaccinated calves were housed together. four other compartments were treated as a whole and 8 compartments were left un ... | 1994 | 7871698 |
| bovine herpesvirus-1 infection reduces bronchial epithelial cell migration to extracellular matrix proteins. | repair of airway epithelium after viral infection involves migration of epithelial cells to cover injured, denuded areas. we determined whether viral infection reduces the capability of bronchial epithelial cells to migrate and to attach to extracellular matrix proteins. inoculation of bovine bronchial epithelial cells in vitro with bovine herpesvirus-1 reduced their ability to migrate in two different assays of cell migration. when attachment assays were performed, fewer cells attached to both ... | 1995 | 7864142 |
| a subunit giv vaccine, produced by transfected mammalian cells in culture, induces mucosal immunity against bovine herpesvirus-1 in cattle. | a truncated version of bovine herpesvirus-1 (bhv-1) glycoprotein iv (tgiv) was produced in a novel, non-destructive expression system based upon regulation of gene expression by the bovine heat-shock protein 70a (hsp70) gene promoter in madin darby bovine kidney (mdbk) cells. in this system, up to 20 micrograms ml-1 of secreted tgiv, which is equivalent to the yield from 4 x 10(6) cells, was produced daily over a period of up to 18 days. different doses of tgiv were injected intramuscularly into ... | 1994 | 7856294 |
| rapid detection of bovine herpesvirus 1 (bhv 1) using the polymerase chain reaction. | a polymerase chain reaction (pcr) assay based on primers from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size was amplified using dna from herpesviruses isolated from reindeer, red deer and goats. the pcr assay was able to detect virus in nasal swabs up to 14 days after experimental infection of cattle and there was a good ... | 1994 | 7839585 |
| synthesis and processing of bovine herpesvirus-1 glycoprotein h. | the translation product of the bovine herpesvirus-1 (bhv-1) gh gene was identified and characterized. synthetic peptides were used to generate specific antisera and a glycoprotein of 108k was precipitated by one of the antisera. cross-immunoprecipitations with monoclonal antibodies to bhv-1 glycoprotein gp108 and the anti-gh peptide antiserum demonstrated that gp108 is the translation product of the gh open reading frame. glycoprotein gh synthesis and intracellular processing was analyzed in inf ... | 1995 | 7831821 |
| the role of the major tegument protein vp8 of bovine herpesvirus-1 in infection and immunity. | the tegument of bovine herpesvirus-1 (bhv-1) carries an abundant protein of 96 kda, termed vp8. immunolabeling using vp8-specific antiserum and colloidal gold-labeled protein a as the electron-dense marker was used to identify vp8 in the virions and virus-infected cells. vp8 was confirmed to be a tegument protein that, like the herpes simplex virus-1 homologue vp13/14, contains o-linked carbohydrates. vp8 was found in the nucleus of virus-infected cells as early as 2 hr postinfection. since vp8 ... | 1995 | 7831797 |
| [development of pcr tests for the detection of bovine herpesvirus-1, bovine respiratory syncytial viruses and pestiviruses]. | the development of pcr assays for detection of bhv-1, brsv, bvdv and another pestiviruses is summarized. a polymerase chain reaction assay based on primers selected from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size (468 bp) was amplified using dna from herpesviruses isolated from reindeer, red deer and goat. the pcr ass ... | 1994 | 7817501 |
| quantitative assessment of the specific cd4+ t lymphocyte proliferative response in bovine herpesvirus 1 immune cattle. | we quantified the cd4+ t cell proliferation specific for bovine herpesvirus 1 (bhv-1) in peripheral blood mononuclear cells from cattle. the stimulation index as detected in proliferative assays performed in the presence of bhv-1 antigen is highly variable in immune cattle. by using proliferative assays performed after negative selection we showed that, as expected, cd4+ t cells were the limiting cell type for antigen-induced proliferation. neither b, gamma delta t nor cd8+ cells seemed to be in ... | 1994 | 7810061 |
| amplification of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymidine kinase region. | a primer pair was designed from the published nucleotide sequence of the coding region of the bovine herpesvirus 1 (bhv-1) thymidine kinase (tk) gene for use in detection of the virus by use of polymerase chain reaction (pcr) amplification of 12 bhv-1 strains (3 atcc and 9 local isolates). a tk deletion mutant bhv-1, and 2 bhv-4 strains from atcc were used as negative controls. one strain each of feline herpes-virus, equine herpesvirus, and bovine adenovirus, and 2 noninoculated bovine cultured ... | 1994 | 7802385 |
| safety aspects in the development of an infectious bovine rhinotracheitis marker vaccine. | clinical trials in cattle demonstrated that the ibr marker modified live vaccine based on the ge-deleted ibr strain difivac is immunogenic and safe for bovines of all ages. potential effects of the vaccine virus have also been tested in swine and sheep and proved safe for these species as well. for evaluation of other environmental aspects, the spread of the vaccine virus after immunisation was investigated. the data indicated that the vaccine virus may be shed by immunised animals but that it h ... | 1995 | 7796970 |
| an inactivated vaccine based on a glycoprotein e-negative strain of bovine herpesvirus 1 induces protective immunity and allows serological differentiation. | the bovine herpesvirus 1 (bhv1) strain za is a conventionally attenuated strain with a 2.7 kb deletion that encompasses the complete coding region for glycoprotein e (ge). this ge-negative strain was used as whole-virus antigen in an inactivated virus vaccine. three different antigen concentrations of this vaccine were evaluated for safety and efficacy in a vaccination-challenge experiment in calves. no adverse effects were observed in any of the calves vaccinated with the ge-negative vaccines. ... | 1995 | 7793128 |
| nucleotide sequence analysis of a 30-kb region of the bovine herpesvirus 1 genome which exhibits a colinear gene arrangement with the ul21 to ul4 genes of herpes simplex virus. | we report the nucleotide sequence of the 19-kb hindiii fragment b of bovine herpesvirus 1 (bhv-1) dna and adjacent parts of the hindiii a and l fragments, which together span a still completely uncharted 30-kb region located between the glycoprotein h gene and the right end of the unique long segment. the analysis revealed 17 complete open reading frames (orfs) and 2 orfs that were interrupted by potential splice donor and acceptor sites. all of these orfs exhibited strong amino acid sequence ho ... | 1995 | 7793062 |
| lectin histochemistry of normal and herpesvirus-infected bovine nasal mucosa. | proliferation of pasteurella haemolytica serotype 1 in the nasal cavity following stress or viral infection is an important event in the pathogenesis of bovine pneumonic pasteurellosis. enhanced adhesion of p. haemolytica to nasal mucosa could be one factor that predisposes animals to this proliferation. nasal mucosa from normal and bovine herpesvirus-1 (bhv1)-infected cattle were examined histochemically for their glycoconjugate composition. twenty lectins were screened, six of which were chose ... | 1995 | 7771054 |
| nucleotide sequence of infectious laryngotracheitis virus (gallid herpesvirus 1) icp4 gene. | the infectious laryngotracheitis virus (iltv) gene encoding a homologue to the icp4 protein of herpes simplex virus (hsv) has been mapped to the inverted repeat region. the complete nucleotide sequence of iltv icp4 has been determined. the iltv orf encoding icp4 is 4386 nucleotides long, calculated from the first of four atg codons, and has an overall g+c content of 59%. the iltv icp4 contains two domains of high homology which have been reported in other studies to be conserved in the icp4 homo ... | 1995 | 7762292 |
| glycoprotein b of bovine herpesvirus-1 binds heparin. | many members of the herpesvirus family have been shown to bind heparan sulfate on the surface of target cells. a previous report on bovine herpesvirus-1 (bhv-1) glycoprotein interactions indicated that only gc bound to heparin-agarose columns. in this report, we demonstrated that both gc and gb of wild-type bhv-1 bound to heparin-agarose columns and that gc-minus virus infectivity was inhibited by heparin. | 1995 | 7747474 |
| characterization of bovine herpesvirus 1 ul49 homolog gene and product: bovine herpesvirus 1 ul49 homolog is dispensable for virus growth. | the sequence of the bovine herpesvirus 1 (bhv-1) gene that is homologous to the herpes simplex virus ul49 gene was determined. the bhv-1 ul49 homolog open reading frame consists of 774 bp and is capable of encoding 258 amino acids. northern (rna) blot analysis showed that the bhv-1 ul49 homolog is transcribed into a 1.1-kb rna which is coterminal with the transcripts of an upstream ul49.5 homolog gene. rabbit antisera produced against synthetic peptides of the predicted ul49 homolog gene product ... | 1995 | 7745736 |
| evidence of persistent malignant catarrhal fever infection in a cow obtained by nucleic acid hybridisation. | a natural non-fatal case of sheep-associated malignant catarrhal fever in a cow is reported. viral dna confirmed by nucleic acid hybridisation and polymerase chain reaction persisted in peripheral blood leukocytes for at least 3 months after the clinical onset of the disease. concultivation of leukocytes with various cell cultures failed to result in isolation of the virus either by cytopathic effect or by nucleic acid hybridisation. | 1994 | 7745589 |
| cloning of hindiii digested bovine herpesvirus-1 dna fragments from an indian respiratory isolate. | the dna from an indian isolate of bovine herpesvirus-1 was isolated and analysed with restriction endonucleases. on shot gun cloning seven hindiii digested bhv-1 dna fragments could be cloned in pbr322 vector. recombinant clones with viral dna insert was identified by insertional inactivation of the selection marker and restriction analysis of recombinant plasmids with hindiii. further, recombinant plasmids were analysed with hindiii, ecori and bamhi restriction endonucleases to identify the dif ... | 1995 | 7735131 |
| excretion of bovine herpesvirus 1 in semen is detected much longer by pcr than by virus isolation. | to compare the sensitivities of pcr and virus isolation and to examine the course of virus excretion in semen, we intrapreputially inoculated eight bulls with bovine herpesvirus 1 (bhv1) and used two bulls as sentinels. from these bulls, we collected a large panel of semen samples during 65 days postinfection (dpi). at 44 dpi the bulls received dexamethasone to reactivate putatively latent virus. we analyzed the semen samples by virus isolation on egg yolk-extended semen (vie test), by virus iso ... | 1995 | 7714184 |
| a seroepidemiological study of the importance in cow-calf pairs of respiratory and enteric viruses in beef operations from northwestern quebec. | serum antibody analyses for bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), bovine coronavirus (bcv), and bovine rotavirus (brv) were performed on 527 randomly selected cows, before calving, and on 407 three-week-old calves. in cows and calves, bcv and brv were the most seroprevalent viruses (80% to 100% according to virus and vaccination status). bovine respiratory syncytial virus was the least seroprevalent in the cows, independ ... | 1995 | 7704839 |
| a comparison of restrictive endonuclease sites of bovine herpesvirus type 1 isolates in chile. | the frutillar 82, f nasal 90, f vaginal 90, puente alto 77, vmlb 79, and bio bio 92 isolates of bovine herpesvirus 1 (bhv-1) from chile were analysed by eco ri, bam hi and hind iii restriction enzymes (re). the los angeles (la) strain was used as reference. the results indicated that the fingerprints of f nasal 90 and f vaginal 90 were identical to the three re used, but they were different from the other isolates. major differences were found for puente alto 77 and vmlb 79 isolates with eco ri, ... | 1994 | 7701858 |
| antigenic differences between the major glycoproteins of bovine herpesvirus type 1.1 and bovine encephalitis herpesvirus type 1.3. | differences in the antigenic structure of the major glycoproteins, gi, giii and giv, of bovine herpesvirus type 1.1 (bhv1.1) and the neurovirulent bhv1.3 were demonstrated with a panel of monoclonal antibodies (mabs) prepared against the bhv1.1 glycoproteins. glycoprotein giii of bhv1.3 was the most dissimilar, reacting with only four of 15 giii-specific mabs. glycoproteins gi and giv of bhv1.3 reacted with eight of 11 and eight of 12 specific mabs, respectively. monospecific bovine antisera to ... | 1993 | 7688409 |
| expression of glycoprotein giii-human decay-accelerating factor chimera on the bovine herpesvirus 1 virion via a glycosyl phosphatidylinositol-based membrane anchor. | mutants of bovine herpesvirus 1 that express a truncated envelope glycoprotein giii or a giii-human decay-accelerating factor (hdaf) chimeric protein (giii.hdaf) were employed to evaluate the function of the transmembrane and cytoplasmic domains of the giii molecule. truncated giii (i.e., lacking the transmembrane and cytoplasmic region) was readily released from infected cells and was not detected on mature virus particles. in contrast, replacement of the transmembrane and cytoplasmic domains w ... | 1993 | 7687305 |
| mapping of heparin-binding structures on bovine herpesvirus 1 and pseudorabies virus giii glycoproteins. | the giii glycoproteins of both bovine herpesvirus 1 (bhv 1) and pseudorabies virus (prv) mediate the initial and dominant interactions between virus and permissive host cells. by studying virus binding to wild-type and heparin-deficient cho cells, we demonstrated that the cellular heparin-like moieties play an essential role in bhv 1 and prv giii-mediated virus attachment. subsequent studies were carried out to map the giii structures that are responsible for heparin binding. first, based on the ... | 1993 | 7683156 |
| bovine viral diarrhea virus: biotypes and disease. | bovine viral diarrhea virus continues to produce significant economic losses for the cattle industry and challenges investigators with the complexity of diseases it produces and the mechanisms by which it causes disease. this paper updates and attempts to clarify information regarding the roles of noncytopathic and cytopathic bovine viral diarrhea viruses in persistent infections and mucosal disease. it also covers, in brief, what is known of the new diseases: thrombocytopenia and hemorrhagic di ... | 1995 | 7648541 |
| establishment of conditions for the detection of bovine herpesvirus-1 by polymerase chain reaction using primers in the thymidine kinase region. | polymerase chain reaction (pcr) for detection of bovine herpesvirus-1 (bhv-1) was developed and optimized using 22 bp sense and 20 bp antisense primers in the thymidine kinase (tk) coding region. the amplification product is 183 bp long. the pcr optimization was done using bhv-1 tissue culture supernate (bhv-1tcs), concentrated bhv-1 tissue culture supernate (cbhv-1tcs) and sucrose gradient purified bhv-1 (pbhv-1). the sensitivity of four methods of sample preparation which are standard dna extr ... | 1995 | 7648533 |
| comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (bhv-1) in artificially infected bovine semen. | bovine semen samples spiked with bovine herpesvirus 1 (bhv-1) were used to compare dot blot hybridization, polymerase chain reaction (pcr), and virus isolation for detection of bhv-1 in bovine semen. the pcr amplification used primers targeting the bhv-1 thymidine kinase gene and a nucleic acid releasing cocktail (genereleaser); the pcr product was used as the dna probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (bft) cell cultures. semen diluted 1:20 in t ... | 1995 | 7648521 |
| effect of recombinant bovine interleukin-1 beta on viral/bacterial pneumonia in cattle. | bovine herpesvirus-1 (bhv-1) is an important pathogen of respiratory infections in cattle. its continuing importance lies in its ability to predispose infected hosts to bacterial infections (e.g., pasteurella haemolytica). in this study we determined whether the immunoregulatory effects induced by recombinant bovine interleukin-1 (rbil-1) could stimulate appropriate host defense mechanisms to influence the course of bhv-1 and p. haemolytica infection in cattle. we first evaluated the effect of m ... | 1995 | 7648445 |
| identification of gene products encoded by the latency-related gene of bovine herpesvirus 1. | bovine herpesvirus 1 (bhv-1) establishes a latent infection in sensory ganglionic neurons of infected animals. expression of latency-related (lr) gene products is controlled by a 980-bp fragment (lr promoter). dna sequence analysis revealed that two major open reading frames (orfs) are in the lr gene. antibodies directed against both orfs were generated in rabbits by using synthetic peptides. antibody p2, which is directed to sequences near the amino terminus of orf 2, recognized a 41-kda protei ... | 1995 | 7636978 |
| the bovine herpesvirus alpha gene trans-inducing factor activates transcription by mechanisms different from those of its herpes simplex virus type 1 counterpart vp16. | in herpes simplex virus (hsv)-infected cells, viral gene expression is initiated when the immediate-early, or alpha, genes are transactivated by the alpha gene trans-inducing factor (alpha tif), a component of the infecting virion. the protein binds to one or more recognition elements (taatgarat) in the promoters of alpha genes via interaction with the cellular proteins oct-1 and cff. the alpha tif of hsv (hsv-alpha tif) is believed to subsequently accelerate the assembly of the transcription co ... | 1995 | 7636962 |
| [behavior of orf virus in permissive and nonpermissive systems]. | dogs were immunized i.m. with attenuated poxvirus vaccines (vaccinia virus, orf-virus) and a bovine herpesvirus-1 (bhv-1) vaccine. after intradermal (i.d.) application of the vaccine viruses a specific delayed type hypersensitivity (dth) reaction of the skin occurred only with vaccinia virus. the i.d. application of orf-virus caused a short-term, non-specific inflammatory reaction of the skin, even in dogs not immunized with orf-virus. out of 30 sera from orf-virus immunized beagles (n = 4) only ... | 1995 | 7624861 |
| the use of a non-specific defence mechanism inducer in calves exposed to bovine herpesvirus-1 infection: preliminary trials. | the efficacy of an immunomodulator, the baypamun (bayer ag), was tested in calves which were subsequently exposed to bovine herpesvirus-1 (bhv-1) infection. two groups of calves, of 8 animals each, were used for two trials. in one trial, 4 calves were treated with the immunomodulator and the remaining 4 were left as untreated controls. two hours after the first injection of baypamun, all calves, i.e. the treated and the untreated ones, were challenged with bhv-1. comparing them to the untreated ... | 1995 | 7621672 |
| diagnosis of encephalitic bovine herpesvirus type 5 (bhv-5) infection in cattle: virus isolation and immunohistochemical detection of antigen in formalin-fixed bovine brain tissues. | 1995 | 7619909 | |
| sequence characteristics of a gene in infectious laryngotracheitis virus homologous to glycoprotein d of herpes simplex virus. | an infectious laryngotracheitis virus (iltv, gallid herpesvirus 1) gene homologous to glycoprotein d of herpes simplex virus (hsv) was identified and characterized by its nucleotide and derived amino acid sequence. the iltv gd gene is located in the unique short region (u(s)) and contains an open reading frame capable of specifying a polypeptide of 380 amino acids, including n- and c- terminal hydrophobic domains consistent with signal and anchor regions respectively, and no potential sites for ... | 1995 | 7612933 |
| bovine herpesvirus 1 in semen of bulls and the risk of transmission: a brief review. | outbreaks of bovine herpesvirus 1 (bhv1) infections in artificial insemination centres can run a clinical or subclinical course. clinical signs vary from mild to severe balanoposthitis and may be associated with a decrease in semen quality. after intranasal as well as intrapreputial infection, bhv1 can replicate in the preputial and penile mucosae, although the pattern of virus shedding in semen differs considerably per individual bull. beyond the primary phase of a genital infection, bhv1 remai ... | 1995 | 7610554 |
| characterization of cell-binding properties of bovine herpesvirus 1 glycoproteins b, c, and d: identification of a dual cell-binding function of gb. | previous studies have suggested that the attachment of bovine herpesvirus 1 (bhv-1) to permissive cells is mediated by its major glycoproteins b (gb), c (gc), and d (gd). in order to gain further insight into the mechanism of the bhv-1 attachment process, we purified authentic gb, gc, and gd from bhv-1-infected cells and membrane anchor-truncated, soluble gb, gc, and gd from stably transfected cell lines by affinity chromatography and examined their cell-binding properties on madin-darby bovine ... | 1995 | 7609042 |
| study of neutralizing monoclonal antibodies to bovine herpes virus type-1 (cooper strain) by immunoperoxidase and immunoelectron microscopy. | three neutralizing monoclonal antibodies (mabs) that are specific against bovine herpes virus type-1 (bhv-1) were studied as to their viral specificity by immunoperoxidase and immunoelectron microscopy. microscopic examination of gbk bhv-1 infected cells revealed peroxidase activity represented by red-brown granular deposits in the nucleus and cytoplasm. no immunoperoxidase activity was observed in negative controls. for the ultrastructural observations, two approaches were used. firstly we test ... | 1995 | 7599599 |
| cloning of 2.4 kb bovine herpesvirus-1 dna fragment containing glycoprotein iii gene into puc18 plasmid vector. | a recombinant pbr322 plasmid containing bovine herpesvirus-1 hindiii 'i' fragment was analysed using ecori and bamhi restriction endonucleases. this recombinant plasmid was labelled with [alpha 32p]datp and hybridized with southern blot of hindiii digested bhv-1 dna fragments. a 2.4 kb double digested ecori-bamhi fragment of hindiii 'i' was subcloned into puc18 plasmid to get complete giii gene. the recombinant puc18 plasmid was analysed for 2.4 kb bhv-1 dna insert by restriction digestion with ... | 1995 | 7590952 |
| abortion in heifers inoculated with a thymidine kinase-negative recombinant of bovine herpesvirus 1. | the copper isolate of bovine herpesvirus 1 (bhv-1) was used to produce a thymidine kinase-negative (tk-) recombinant by insertion of a beta-galactosidase (bgal) expression cassette into the tk coding region. the recombinant virus (tk- bgal+) was tested for abortifacient activity in cattle by inoculation of 5 pregnant heifers at 25 to 29 weeks gestation. five additional heifers were inoculated with the cooper tk-positive (tk+) virus to serve as controls. after inoculation, both groups of heifers ... | 1995 | 7574153 |
| sequence analysis of the ul39, ul38, and ul37 homologues of bovine herpesvirus 1 and expression studies of ul40 and ul39, the subunits of ribonucleotide reductase. | we sequenced the region of the bovine herpesvirus type 1 (bhv-1) genome corresponding to map units 0.172-0.230 (7964 bp), representing the ul39, ul38, and ul37 homologues of herpes simplex virus which encode the large subunit of ribonucleotide reductase (rr) and components of the viral capsid and the tegument, respectively. to discriminate between two potential initiator augs of the ul39 gene, the 5' end of the mrna was mapped by s1 nuclease protection assays. comparison of the amino acid sequen ... | 1995 | 7571445 |
| a glycoprotein e deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild-type virus, but for a shorter period. | to gain insight into the role of glycoprotein e of bovine herpesvirus 1 (bhv-1), we compared the distribution of wild-type (wt) bhv-1 with that of a ge deletion mutant (ge-) in calves after intranasal inoculation. the wt-infected calves had severe clinical signs, but the ge(-)-infected calves were virtually free of clinical signs. at 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one calf from each group was killed and tissues were collected for virus isolation and pcr analysis. at 3, ... | 1995 | 7561782 |
| restriction endonuclease analysis of bovine herpesvirus type 1 isolates from calves with fatal encephalitis: comparison with vaccine virus. | meningo-encephalitis in feedlot cattle sporadically occurred in the tokachi area in northern japan. the calves had been vaccinated intranasally with a mixed live-vaccine (infectious bovine rhinotracheitis virus, bovine viral diarrhea-mucosal disease virus, and parainfluenza 3 virus) for which intramuscular inoculation was indicated. two additional live vaccines, bovine adenovirus type 7 and bovine respiratory syncytical virus, had been inoculated simultaneously. eleven isolates of bovine herpesv ... | 1995 | 7548427 |
| fine mapping of bovine herpesvirus-1 (bhv-1) glycoprotein d (gd) neutralizing epitopes by type-specific monoclonal antibodies and sequence comparison with bhv-5 gd. | overlapping fragments of the bovine herpesvirus-1 (bhv-1) glycoprotein (gd) orf were expressed as trpe-gd fusion proteins in escherichia coli to map linear neutralizing epitopes defined by bhv-1-specific mabs. the mabs 3402 and r54 reacted with the expressed fragments on western blots that located the epitopes between the amino acids 52-126 and 165-216, respectively, of gd. bovine covalescent sera with high neutralizing antibody titers against bhv-1 reacted with these bacterially expressed prote ... | 1995 | 7530392 |
| a simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1. | by using a monoclonal antibody directed against an epitope located on glycoprotein b of bovine herpesvirus 1 (bhv1), a simple, convenient blocking enzyme-linked immunosorbent assay (elisa) which combines a high sensitivity with a low false-positive rate has been developed. the test can be performed at low variance on undiluted bovine serum samples. the epitope on glycoprotein b appears to be conserved, because it could be detected by immunostaining in all of 160 bhv1 isolates originating from 10 ... | 1994 | 7529249 |
| epitope-specific antibody responses in market-stressed calves to bovine herpesvirus type 1. | reciprocal competition elisa (rcelisa) was conducted to map monoclonal antibodies (mabs) reactive with gi, giii and giv glycoproteins of bovine herpesvirus type 1 (bhv-1) into epitope groups. mabs to glycoproteins gi and giv were divided into six epitope groups each, while giii mabs had been previously divided into four areas. mabs were chosen from each epitope group to compete in celisa wih bovine sera collected during a typical regimen of vaccination and transportation from farm to auction to ... | 1994 | 7526569 |
| isotype-specific antibody responses in sera and mucosal secretions of calves experimentally infected with bovine herpesvirus 1. | enzyme-linked immunosorbent assays (elisas) were developed for studying the kinetics of isotype-specific antibody responses in sera, nasal, ocular and genital secretions of calves infected with bovine herpesvirus 1 (bhv1). the bhv1-specific igm and iga antibodies were measured in antibody capture assays, and the igg1 and igg2 antibodies in indirect double antibody sandwich assays. the elisas were shown to be isotype-specific, sensitive and reproducible. antibodies of all isotypes were able to ne ... | 1995 | 7502487 |
| monoclonal antibodies that distinguish between encephalitogenic bovine herpesvirus type 1.3 and respiratory bovine herpesvirus type 1.1. | seven mouse hybridoma cell lines producing monoclonal antibodies (mab) against an encephalitogenic strain of bovine herpesvirus type 1.3 (bhv-1.3) were established. the clones producing mab were selected to be specific for bhv-1.3 by enzyme-linked immunosorbent assay. only l1b neutralized virus without complement. with the addition of complement, five of the mab neutralized bhv-1.3 but not the respiratory strain bhv-1.1. the anti-bhv-1.3-specific mab q10b, l6g, and l1b precipitated glycoproteins ... | 1994 | 7496928 |
| molecular basis of antigenic variation between the glycoproteins c of respiratory bovine herpesvirus 1 (bhv-1) and neurovirulent bhv-5. | herpesvirus glycoprotein c (gc) functions as a major virus attachment protein. the gc sequence of the neurovirulent bovine herpesvirus type 5 (bhv-5) virus was determined and compared with the gc sequence of the nonneurovirulent bhv-1. alignment of the predicted amino acid sequences of bhv-1 and bhv-5 gc orfs showed that the amino-terminal third of the protein differed between the two viruses. whole or subgenomic fragments of gc coding regions from both viruses were expressed as trpe-gc fusion p ... | 1995 | 7491780 |
| interferon-alpha primes macrophages for lipopolysaccharide-induced apoptosis. | apoptosis plays an important role in generating and maintaining an effective immune system. many pathogens can perturb the homeostasis of the immune system by either inducing or suppressing cell death of immune cells. using bovine macrophages as a model, we found that interferon-alpha, one of the host's responses to viral infection, can prime macrophages for activation-induced apoptosis. exposure of bovine bone-marrow-derived macrophages to interferon-alpha and subsequent activation with lipopol ... | 1995 | 7488062 |
| gp13 (ehv-gc): a complement receptor induced by equine herpesviruses. | equine herpesviruses type 1 (ehv-1) and type 4 (ehv-4) induce a complement receptor protein on the surface of infected cells capable of binding to the third component of complement (c3). the protein mediating the binding to the c3 component of complement was identified as glycoprotein 13 (gp13, ehv-gc), as expression of the cloned viral gene under the control of a cmv promoter induced c3 binding activity at the transfected cell surface. comparable to glycoprotein c (gc) from herpes simplex virus ... | 1995 | 7483825 |
| bhv-1 glycoprotein 1 and recombinant interleukin 1 beta efficiently elicit mucosal iga response. | the mucosal immune response to most soluble antigens administered directly to the mucosal system is low and requires a large amount of antigen and frequent vaccinations. in this study we tested whether immunizing cattle at a site which shares lymphatic drainage with the nasal mucosa could prime local mucosal immunity. we further tested whether recombinant bovine il-1 beta (rboil-1 beta) could potentiate the induction of mucosal immunity. animals were immunized subcutaneously at the base of the e ... | 1995 | 7483811 |
| antibody responses by cattle after vaccination with commercial viral vaccines containing bovine herpesvirus-1, bovine viral diarrhea virus, parainfluenza-3 virus, and bovine respiratory syncytial virus immunogens and subsequent revaccination at day 140. | calves were vaccinated with four different commercial viral vaccines containing bovine herpesvirus-1 (bhv-1), bovine viral diarrhea (bvdv), parainfluenza-3 virus (pi-3v), and bovine respiratory syncytial virus (brsv) immunogens. for the initial vaccination certain vaccines were given twice (days 0 and 28), whereas other vaccines were given on day 0. the calves received another injection on day 140 with the vaccine originally given on day 0. the sera were collected at days 0, 7, 14, 21, 28, 42, 5 ... | 1995 | 7483787 |
| identification and transcriptional analysis of a 3'-coterminal gene cluster containing ul1, ul2, ul3, and ul3.5 open reading frames of bovine herpesvirus-1. | we have identified and sequenced 3113 nucleotides located at the right end of the hindiii l fragment of the bovine herpesvirus-1 genome from map units 0.712 to 0.734. analysis of the sequence identified four open reading frames (orfs) which are designated ul1, ul2, ul3, and ul3.5 based on their homology with proteins of herpes simplex virus-1 (hsv-1), pseudorabies virus (prv), equine herpesvirus-1, and varicella-zoster virus. the ul1 orf of 158 amino acids exhibits limited homology with ul1 (gly ... | 1995 | 7483276 |
| the bovine herpesvirus 1 maturational proteinase and scaffold proteins can substitute for the homologous herpes simplex virus type 1 proteins in the formation of hybrid type b capsids. | we determined the nucleotide sequence of a 3.5-kb region of the bovine herpesvirus 1 (bhv-1) genome which contained the complete bhv-1 homologs of the herpes simplex virus type 1 (hsv-1) ul26 and ul26.5 genes. in hsv-1, the ul26 and ul26.5 open reading frames encode scaffold proteins upon which viral capsids are assembled. the ul26-encoded protein is also a proteinase and specifically cleaves both itself and the ul26.5-encoded protein. the overall bhv-1-encoded amino acid sequence showed only 41 ... | 1995 | 7474173 |
| the pathological features of severe cases of infectious bovine rhinotracheitis. | in recent outbreaks of infectious bovine rhinotracheitis (ibr) in britain a proportion of the animals affected developed a severe clinical disease characterised, at necropsy, by widespread damage to the respiratory tract. they had necrotising rhinitis, pharyngitis, laryngotracheobronchitis with extensive pseudomembrane formation and severe pneumonia with or without interstitial emphysema. renal infarction was seen in approximatley half of the cases. the central nervous system was not affected in ... | 1980 | 7456296 |
| isolation of bovine herpesvirus-1 from trigeminal ganglia of clinically normal cattle. | trigeminal ganglia collected from 56 adult dairy cattle at slaughter were maintained in explant culture. bovine herpesvirus-1 was isolated from six ganglia after periods of 7 to 24 days' maintenance in vitro. antibody against bovine herpesvirus-1, indicating exposure to that virus, was found in 45.4% of the sample population from which ganglia were collected. | 1980 | 7447114 |
| failure of a subunit bovine herpesvirus 1 vaccine to protect against experimental respiratory disease in calves. | in two experiments in which 31 calves were used, a bovine herpesvirus 1 subunit vaccine previously shown to elicit a strong immunological response in adult cattle failed to do so in younger animals and failed to protect against pneumonia caused by sequential exposure to virulent bovine herpesvirus 1 and pasteurella haemolytica aerosols. one of the experimental groups had been previously inoculated with a live commercial vaccine but even this failed to elicit a strong immunological response. thes ... | 1981 | 7272846 |
| detection of bacteremia in cattle inoculated with bovine viral diarrhea virus. | bovine viral diarrhea virus infection of cattle interfered with normal blood clearance mechanisms, as evidenced by the detection of an endogenous bacteremia in up to 85% of infected calves during the first 5 days after infection. the occurrence of detectable bacteremia correlated with the period of leukopenia and depression of lymphocyte responses to mitogens. noninoculated control animals, reinoculated immune calves, or calves inoculated with infectious bovine rhinotracheitis virus yielded cons ... | 1981 | 7258771 |
| scanning electron microscopy of the tracheal epithelium of calves inoculated with bovine herpesvirus 1. | tracheal epithelium was examined by light, scanning electron and transmission electron microscopy at four, five and seven days after calves were infected intranasally with bovine herpesvirus. similar areas were examined from uninoculated calves. tracheal epithelial lesions were found in all inoculated calves and their degree of severity did not vary with the interval between inoculation and examination. extensive loss of cilia leaving areas of tracheal epithelium covered by microvilli was the ma ... | 1980 | 7255893 |
| herpesvirus-induced "early" glycoprotein: characterization and possible role in immune cytolysis. | glycoprotein gvp-11 (molecular weight, 71,500), induced by bovine herpesvirus type 1, was detected on the external surface of infected cells. it could be categorized as an "early" or "beta" class protein since it was synthesized early in the infectious process and its expression was not dependent upon prior viral dna replication in the infected cells. monoclonal antibodies directed against gvp-11 immunoprecipitated that glycoprotein and some low-molecular-weight polypeptides from infected cells ... | 1982 | 7143563 |
| effect of lymphocytes and broncho-alveolar washing cells on the replication of bovine herpesvirus type 1 in tracheal organ cultures. | the daily addition of lymphocytes collected from a calf between 7 and 11 days after experimental infection with bovine herpesvirus type 1 (bhv-1) to bovine fetal tracheal organ cultures after infection with bhv-1 did not inhibit virus replication. the daily addition of normal lymphocytes, together with a low concentration of serum antibody against bhv-1, had a slight viral inhibitory effect which was believed to be due to antibody-dependent cell-mediated cytotoxicity. the addition of broncho-alv ... | 1982 | 7112897 |
| effect of infectious bovine rhinotracheitis virus infection of calves on cell populations recovered by lung lavage. | calves were challenge exposed with infectious bovine rhinotracheitis (ibr) virus, and experiments were carried out to determine the presence of virus in the lungs, virus or viral antigen in alveolar macrophages, and alterations in immune functions of alveolar macrophages. in experiment 1, calves were challenge exposed intranasally with ibr virus. although clinical signs of ibr occurred in all challenge-exposed calves, there was minimal evidence of virus or viral antigen in cells lavaged from the ... | 1982 | 7103198 |
| distribution of pasteurella haemolytica and pasteurella multocida in the bovine lung following vaccination and challenge exposure as an indicator of lung resistance. | experimental calves were vaccinated with virulent strains of pasteurella haemolytica or pasteurella multocida or with phosphate-buffered saline solution either by an aerosol method or by subcutaneous injection. calves were subsequently challenge exposed by intrapulmonic inoculation of the homologous virulent pasteurella species. sections obtained from the resulting pulmonic lesion were stained, using a fluorescent antibody technique, to determine relative number, location, and integrity of the c ... | 1982 | 7073058 |
| pathological changes in young and adult cattle after intranasal inoculation with infectious bovine rhinotracheitis virus. | 1982 | 7068953 | |
| immunologic effects of experimental iodine toxicosis in young cattle. | a study was designed to evaluate effects of 4 dosage levels (0, 50, 250, or 1,250 mg) of daily iodine supplementation on cell-mediated and humoral immune mechanisms in 40 calves, 10 per group, for a 6-month period. immune and inflammatory responses were measured by titers to brucella, leptospira, and infectious bovine rhinotracheitis virus vaccinations, by stimulation of lymphocyte mitoses with poke-weed, phytohemagglutinin (pha), and conconavalin a mitogens, by intradermal pha responses, by in ... | 1980 | 6996540 |
| hematologic, serum chemistry and serologic values of dall's sheep (ovis dalli dalli) in alaska. | in june 1979, 73 dall's sheep were captured near tok, alaska to determine selected hematologic and serum metabolite parameters and to determine the presence of antibodies to selected pathogens. hematology and serum metabolite values were compared with values for domestic sheep and bighorn sheep (ovis canadensis). antibodies were detected against brucella sp. (4%), campylobacter feti (30%), contagious ecthyma virus (23%) and bovine parainfluenza type 3 virus (1%). antibodies were not detected aga ... | 1983 | 6887435 |
| effect of bacterial dose on pneumonia induced by aerosol exposure of calves to bovine herpesvirus-1 and pasteurella haemolytica. | sixteen crossbred hereford range calves were used to study the effects of pasteurella haemolytica aerosols of various concentrations administered 4 days after they were exposed to a uniform aerosol of bovine herpesvirus-1 (bhv-1). all calves were given bhv-1 on day 0. on day 4, groups of 4 calves each were exposed to aerosols of p haemolytica generated from suspensions with the following concentrations: high (10(8)/ml)--group 1, moderate (10(5)/ml)--group 2, low (10(2)/ml)--group 3, or none (con ... | 1983 | 6830012 |
| immunogenicity of a soluble antigen against pasteurella haemolytica-associated pneumonia in calves. | three experiments were performed to evaluate the immunogenic potency of a soluble fraction of pasteurella haemolytica against pneumonic pasteurellosis in calves. a soluble antigen was extracted by a 2.5% saline solution from p. haemolytica. weaned holstein bull calves, seronegative for infectious bovine rhinotracheitis virus ( ibrv ) and the pasteurella antigen, were vaccinated either by repeated subcutaneous (sc) vaccination, or by exposure 3 times to the aerosol of p. haemolytica antigen. chal ... | 1984 | 6740917 |
| evidence for defective neutrophil function in lungs of calves exposed to infectious bovine rhinotracheitis virus. | calves were exposed to an aerosol of infectious bovine rhinotracheitis (ibr) virus followed five days later by an aerosol of pasteurella haemolytica. the animals were subjected to bronchoalveolar lavage before ibr and four days after, and again at 0, 4, 24, and 48 hours following pasteurella haemolytica challenge. the results of these experiments suggest that neutrophil infiltration into the lung, in response to the presence of the bacteria was delayed thereby allowing the bacteria to become est ... | 1984 | 6719822 |
| diagnostic studies of the fetus, placenta and maternal blood from 265 bovine abortions. | during 1981, the fetus, placenta and maternal serum were received from each of 265 bovine abortions. these specimens were examined using histopathological, histochemical, bacteriological, mycological, endocrinological, immunological, serological and virological techniques. the cause of abortion was identified in 98 (37%) cases. of these diagnosed abortions 27 (28%) were due to infection with fungi, 17 (17%) to salmonella spp, 11 (11%) to campylobacter fetus and 10 (10%) to corynebacterium pyogen ... | 1984 | 6705541 |
| parasites, diseases, and health status of sympatric populations of sika deer and white-tailed deer in maryland and virginia. | in july 1981, investigations on parasites, diseases, and herd health status were conducted on sympatric populations of sika deer (cervus nippon) and white-tailed deer (odocoileus virginianus) from blackwater national wildlife refuge (maryland) and chincoteague national wildlife refuge (virginia) on the delmarva peninsula. five adult deer of each species were collected from each location and subjected to thorough necropsy examinations and laboratory tests. white-tailed deer at both locations harb ... | 1983 | 6644934 |
| inhibition of glycosylation of bovine herpesvirus 1 glycoproteins by the thymidine analog (e)-5-(2 bromovinyl)-2'-deoxyuridine. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was phosphorylated by the bovine herpesvirus 1 (bhv-1)-induced thymidine kinase and subsequently incorporated into viral dna, resulting in dna that was more dense than dna from untreated cells. incorporation of the drug did not result in the termination of replicating bhv-1 dna molecules since radioactively labeled dna synthesized in drug-treated and untreated cells sedimented at similar rates in alkaline sucrose gradients. no differences were observed ... | 1983 | 6614891 |
| characterization of bovine herpesvirus-1 isolated from trigeminal ganglia of clinically healthy cattle. | isolates of bovine herpesvirus-1 (bhv-1) recovered from tissue explants of trigeminal ganglia of clinically healthy cattle were studied in vitro and in an animal model, and their characteristics were compared with those of vaccine and field strains of bhv-1. the isolates could be distinguished by their plaque size on cell monolayers, but were not significantly different in their thermal inactivation profiles at 48 c. temperature-sensitive mutants were not found among the isolates when they were ... | 1984 | 6611096 |
| effect of homologous delayed hypersensitivity testing on specific and non-specific lymphoblastic transformation in cattle latently infected with bovine rhinotracheitis virus (bovine herpesvirus 1, bhv 1). | two cattle latently infected with infectious bovine rhinotracheitis virus (bovine herpesvirus 1, bhv 1) were intradermally injected with inactivated bhv 1 antigen (delayed hypersensitivity test, dht, skin test). another animal, free of bhv 1 infection was similarly treated. two latent carriers of the virus, intradermally injected with phosphate-buffered saline solution were used as control. the evolution of spontaneous multiplication of lymphocytes and lymphoblastic transformation in vitro induc ... | 1984 | 6529117 |
| a preliminary serological survey of viral antibodies in peruvian sheep. | this study reports the sero-prevalence of viral infections in sheep in peru. serum samples were collected from 34 mature healthy rams located in 3 different geographic regions of the country (north, central and south). the sera were tested for antibodies to the following viruses: respiratory syncytial virus (rsv); parainfluenza 3 (pi-3) virus; bovine viral diarrhea/border disease (bvd/bd) virus; bovine herpesvirus 1 (bhv-1); bluetongue (bt) virus; ovine progressive pneumoniae (opp) virus; bovine ... | 1984 | 6528458 |
| animal dna viruses analyzed by colour electron microscopy. | black-and-white and colour transmission electron micrographs of three dna viruses (infectious bovine rhinotracheitis virus, bovine adenovirus type 5 and molluscum contagiosum) are comparatively presented. the colour images have been obtained by an original method of computer-assisted analogic processing of black-and-white micrographs, using the romanian electronic device optimiscop. a comparative analysis of the two types of images demonstrates the variety of additional information provided by t ... | 1984 | 6495647 |
| effect of recombinant dna-produced bovine interferon alpha (boifn-alpha 1) on the interaction between bovine alveolar macrophages and bovine herpesvirus type 1. | treatment of bovine alveolar macrophages (bam) with bovine leukocyte interferon (boifn-alpha 1) resulted in reduced bovine herpesvirus type 1 replication and spread. this was demonstrated by reduced virus yields and number of cells infected. boifn-alpha 1 treatment of bam also induced enhanced fc receptor expression and/or avidity by the cells, increased their activity in antibody-dependent cellular cytotoxicity, and augmented their extrinsic antiviral activity as measured by a reduction in the ... | 1984 | 6470681 |
| replication of five bovine respiratory viruses in cultured bovine alveolar macrophages. | the replication of five bovine respiratory viruses in cultured bovine alveolar macrophages (bam) was investigated. the bovine herpesvirus dn-599 strain did not cause cytophatic effect (cpe), extracellular virus and intracellular antigen were not demonstrated. although a small number of bovine respiratory syncytial virions were consistently released and about 1 percent of the bam were fluorescence antibody (fa) positive the virus caused no cpe. infectious bovine rhinotracheitis virus caused cpe a ... | 1983 | 6404239 |
| interactions of monoclonal antibodies and bovine herpesvirus type 1 (bhv-1) glycoproteins: characterization of their biochemical and immunological properties. | hybridoma cell lines producing monoclonal antibodies to bovine herpes virus type 1 (bhv-1) were established. the monoclonal antibodies were characterized with respect to their antigen specificities and biological activities. one group of eight monoclonal antibodies precipitated the glycoproteins gvp 3 (180k) and gvp 9 (91k), a second group of thirteen monoclonal antibodies precipitated gvp 6 (130k), gvp 11 (74k) and gvp 16 (55k), and one monoclone secreted antibodies specific for gvp 7 (105k). a ... | 1984 | 6377683 |
| anticytotoxin activity of bovine sera and body fluids against pasteurella haemolytica a1 cytotoxin. | toxin neutralizing activity of bovine sera and body fluids against pasteurella haemolytica type a1 cytotoxin was evaluated by 51cr release assay using bovine peripheral blood mononuclear leukocytes as the target cells. sera collected from precolostral calves did not exert anticytotoxin activity at 10(-1) or higher dilutions, whereas randomly selected complement fixing antibody-negative sera neutralized on average over 90% of cytotoxin activity at the 10(-1) dilution and less than 50% of the toxi ... | 1984 | 6372970 |
| mass abortion caused by infectious rhinotracheitis (ibr/ipv) virus in a beef cattle herd. | 1983 | 6331143 | |
| prevalence of bovine herpesvirus-1, bovine viral diarrhea, parainfluenza-3, goat respiratory syncytial, bovine leukemia, and bluetongue viral antibodies in sheep. | sera from healthy sheep were collected in january and march 1982 from flocks of sheep located in southwestern and southeastern louisiana. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the sera were tested also for bovine leukemia virus (blv) and bluetongue virus (btv) antibodies by immunodiffusion tests. the number of flo ... | 1984 | 6329044 |
| reproductive tract lesions in heifers after intrauterine inoculation with infectious bovine rhinotracheitis virus. | cross-breed heifers given infectious bovine rhinotracheitis virus by intrauterine inoculation 1 day after natural mating with a noninfected bull were killed on postinoculation days 4 to 14. when reproductive organs were examined for gross and microscopic lesions and for virus infection, the most severe uterine lesions were found in the body and caudal portions of the uterine horns of heifers killed between postinoculation days 4 and 9. primary pathologic features were necrosis, edema, hemorrhage ... | 1984 | 6329043 |
| isolation of bovine herpesvirus-1 from vesicular lesions of the bovine udder. | bovine herpesvirus-1 was isolated from vesicular lesions on the udder and mammary papillae (teats) of a charolais cow. lesions on the animal consisted of papules and vesicles up to 10 mm in diameter. the virus was identified by fluorescent antibody and serum-neutralization tests. | 1984 | 6329042 |
| effect of bovine viral diarrhea virus infection on the distribution of infectious bovine rhinotracheitis virus in calves. | four calves (control animals) were inoculated (by aerosol) with cooper's strain of infectious bovine rhinotracheitis (ibr) virus given with an ultrasonic nebulizer. seven other calves (principals) were inoculated (by aerosol) with a noncytopathic strain of bovine viral diarrhea (bvd) virus and 7 days later, with ibr virus. the calves were killed 3 to 8 days after the latter virus was inoculated. various tissues were collected from the calves during necropsy and assayed for ibr virus. in the cont ... | 1984 | 6329040 |
| the dna of an ipv strain of bovid herpesvirus 1 in sacral ganglia during latency after intravaginal infection. | two calves were inoculated intravaginally with a strain of bovid herpesvirus type 1 (bhv-1, ibr/ipv) isolated from a cow with infectious pustular vulvovaginitis (ipv). the animals were killed during a latent stage of infection as characterized by seroconversion, absence of virus shedding and recrudescence of virus shedding after dexamethasone treatment. ipv-virus dna was detected in 9 out of 20 sacral ganglia of the 2 calves. of the sections, 7.2% (n = 250) contained 1 cell with ipv-virus dna, w ... | 1984 | 6326378 |