Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the limited role of the human interferon system response to respiratory syncytial virus challenge: analysis and comparison to influenza virus challenge. | the respiratory syncytial virus (rsv)-induced production of interferon (ifn) by human macrophages and mononuclear leukocytes (mnl) and the sensitivities of rsv to subtypes of ifn-alpha were examined and compared to ifn production induced by influenza virus. influenza virus induced high titers of total ifn bioactivity, transcription of the ifn-alpha 1 and ifn-beta gene products and production of ifn-gamma. in contrast, rsv induced minimal or no detectable total ifn activity, and the absence of if ... | 1992 | 1522795 |
| lack of effect of respiratory syncytial virus infection on theophylline disposition in children. | conflicting reports raise a question about decreased plasma clearance (clp) of theophylline in man during viral infections. thus a dilemma exists concerning requisite dose adjustments. we examined this issue by retrospectively evaluating theophylline clp in children infected with respiratory syncytial virus (rsv). two pharmacokinetic approaches were applied to a one-compartment open model to fit theophylline concentrations during 83 hospitalizations of 76 children, 6 to 48 months of age, who rec ... | 1992 | 1517927 |
| improved outcome of respiratory syncytial virus infection in a high-risk hospitalized population of canadian children. pediatric investigators collaborative network on infections in canada. | to determine the outcomes in children at high risk for death or complications from respiratory disease who are hospitalized with respiratory syncytial virus (rsv) infection. | 1992 | 1517907 |
| active respiratory syncytial virus purified by ion-exchange chromatography: characterization of binding and elution requirements. | two viruses, respiratory syncytial virus (rsv) and vesicular stomatitis virus (vsv) were used to evaluate viral purification by an affinity resin column (matrex cellufine sulfate (mcs); amicon division, wr grace & co.). viable rsv was purified significantly from crude cell lysate by a single pass through a column containing the anionic mcs resin. most cell protein and albumin eluted from the mcs resin with phosphate buffered saline (pbs) but rsv eluted at high ionic strength, i.e., greater than ... | 1992 | 1517352 |
| effects of vaccination against 18 immunogens in beef replacement heifers at weaning. | humoral immune responses to vaccination, mean daily body-weight gains, morbidity, and mortality were compared in groups of beef replacement heifers from weaning to 4 months after weaning. the only difference in management among groups of heifers was the number and type of vaccines they received. heifers were vaccinated at weaning (mean age, 205 days) and again 28 days later against 0, 1, 9, 10, 17, or 18 antigens, using commercially available monovalent and multivalent vaccines. the common vacci ... | 1992 | 1517133 |
| evidence of immunosuppression by bovine respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major respiratory pathogen in human infants and calves. calves and lambs infected with bovine rsv show mild clinical signs but they are more susceptible to secondary infection with pasteurella haemolytica. lambs infected with p. haemolytica 6 days after experimental infection with bovine rsv had significantly greater magnitudes of fever, higher disease and lesion scores and higher mortality rates than those infected with p. haemolytica or bovine rsv alone ( ... | 1992 | 1514055 |
| effects of protease inhibitors on replication of various myxoviruses. | we studied the effects of eight protease inhibitors on the multicycle replications of various orthomyxoviruses and paramyxoviruses. among the compounds, nafamostat mesilate, camostat mesilate, gabexate mesilate, and aprotinin, which are widely used in the treatment of pancreatitis, inhibited influenza virus a and b replication at concentrations that were significantly lower than their cytotoxic thresholds in vitro. none of the protease inhibitors had activity against respiratory syncytial virus, ... | 1992 | 1510439 |
| antibody response of calves to immunoaffinity-purified bovine respiratory syncytial virus vp70 after vaccination and challenge exposure. | immunoaffinity-purified bovine respiratory syncytial virus (brsv) fusion (f) protein elicited anti-brsv-specific antibody responses in brsv-seronegative calves. after primary vaccination, all calves seroconverted to brsv as determined by the virus neutralization (vn) test and developed anti-f protein antibodies detectable by protein immunoblot analyses. subsequent vaccinations induced greater than twofold increase in vn titer in 3 of 9 (33%) calves, and 1 calf became vn-negative, but still had n ... | 1992 | 1510306 |
| synthesis and antiviral evaluation of n-carboxamidine-substituted analogues of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine hydrochloride. | ten, hitherto unreported, analogues of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine hydrochloride (2a, ribamidine) and methyl carboximidate 5 have been synthesized. these include the n-cyano (2b), n-alkyl (2c-e), n-amino acid (2f-h), n,n'-disubstituted (6, 7a,b), and the n-methylated carboxamide (1f) analogues of ribavirin. in addition, a new facile synthesis of carboxamidine 2a was also developed. all compounds were evaluated for biological activity against the following rna viruses: p ... | 1992 | 1507208 |
| direct sequencing of baculovirus genomic dna: sequence determination of the engineered respiratory syncytial virus chimeric fg gene. | primer-directed enzymatic sequencing has proven to be an efficient and effective method for sequencing various size double-stranded dna templates. we previously developed a primer-directed sequencing procedure for using double-stranded cosmid (50 kb) dnas as template. we are interested in using this method to directly sequence larger dna templates. towards this goal we applied this method to directly sequence an engineered gene that had been transferred and integrated into the 130-kb baculovirus ... | 1992 | 1503779 |
| comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus. | three enzyme-linked immunosorbent assays (eia) designed for the detection of human respiratory syncytial virus (rsv) were evaluated for the detection of bovine respiratory syncytial virus (brsv) in bovine lungs and the results were compared with those obtained by a direct fluorescent antibody assay (dfa). the eia tests used were directigen eia, kallestad pathfinder eia, and abbott rsv eia. homogenates of lung tissues obtained from 64 cattle that had died of respiratory disease were used; 32 were ... | 1992 | 1501586 |
| use of monoclonal antibodies for rapid diagnosis of respiratory viruses: memorandum from a who meeting. | a monoclonal antibody diagnostic kit (using immunofluorescence) to detect directly viral antigens of respiratory syncytial virus, influenza virus a, influenza virus b, parainfluenza viruses (types 1, 2, and 3), and adenovirus has been developed by who. during 1990 and 1991 a total of 16 laboratories in different parts of the world were invited to test the kit in clinical use. this memorandum summarizes the results obtained as well as the discussions and recommendations made by participants at a ... | 1992 | 1486665 |
| detection of respiratory syncytial virus in acute bronchiolitis in infants. | direct detection of respiratory syncytial (rs) virus antigen in nasopharyngeal secretions (nps) provides the most rapid diagnostic test for rs infection, but more sensitive methods might be more beneficial in the study of virus shedding. rs virus rna was extracted from cells stored at -70 degrees c either in suspension with added rnase inhibitor or as a pellet without inhibitor. the rna was reverse transcribed, the resultant cdna amplified by the polymerase chain reaction and detected by ethidiu ... | 1992 | 1474378 |
| viral infection and alpha interferon in sids. | conventional approaches to virus detection fail to provide convincing evidence in support of a viral aetiology for sids. virus positivity of about 20% in cases of sids is typical of most studies and is similar to that found in control groups. the application of direct immunofluorescence assays (dfa) has increased virus detection rates to 30-40%. constraints imposed by technology, postmortem interval, choice of samples for analysis and the necessary limitation of virus types to be sought may all ... | 1992 | 1474154 |
| susceptibility to infection in relation to sids. | because there is little evidence that invasive bacterial diseases contribute to cot deaths, most studies on infectious causes of sids have focused on viruses or toxin producing bacteria. although epidemiological studies found marginally significant associations between influenza virus and sids, respiratory syncytial virus (rsv) was isolated from 90% of older infants with sids. there are conflicting reports that some toxigenic bacteria (clostridium botulinum, clostridium difficle, enterotoxigenic ... | 1992 | 1474153 |
| molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup a lineages. | methods for the rapid analysis of samples of respiratory syncytial (rs) virus are described using the polymerase chain reaction (pcr) followed by restriction mapping. isolates (either clinical samples or tissue culture grown virus) can readily be divided into subgroups and then further classified into lineages. these methods enable examination of large numbers of isolates by molecular techniques, thereby facilitating research into the molecular epidemiology of the virus. | 1992 | 1474135 |
| radiographic and radionuclide lung perfusion imaging in healthy calves and calves naturally infected with bovine respiratory syncytial virus. | nine calves between three and 18 weeks old with serologically confirmed natural bovine respiratory syncytial virus infection were examined clinically, radiographically and by radionuclide lung perfusion imaging. the results were compared with those from seven healthy calves. the diseased calves were euthanased and examined pathologically, virologically and bacteriologically. the clinical signs indicated that the disease was in an acute stage. radiography of the diseased animals revealed cysts, c ... | 1992 | 1471325 |
| pneumonia--a clinical or radiographic diagnosis? etiology and clinical features of lower respiratory tract infection in adults in general practice. | etiology and clinical manifestations have been studied in 153 adult patients with lower respiratory tract infection, and the results are presented according to clinical and radiographic diagnosis. laboratory investigations revealed that bacterial infection, mycoplasma and chlamydia included, occurred as often in 22 patients whose clinical diagnoses of pneumonia were not evident radiographically, as in 20 patients with radiographic pneumonia. in the latter group significantly higher values of ery ... | 1992 | 1465584 |
| shedding of infectious virus and virus antigen during acute infection with respiratory syncytial virus. | shedding of respiratory syncytial virus (rsv) in nasopharyngeal aspirates (npa) of hospitalized children with acute respiratory infection was studied using direct antigen detection by time-resolved fluoroimmunoassay, rapid identification of infectious virus in centrifugally inoculated cell cultures by immunoperoxidase staining and conventional virus culture. sequential npas, in which also local rsv-specific iga response was measured, were collected from children with proven rsv infection. the sh ... | 1992 | 1460457 |
| [the use of a virazole aerosol in young children with a respiratory syncytial virus infection]. | the therapeutic effect of aerosol virasol was studied in young infants suffering from rs virus infection with concomitant broncho-obstructive syndrome. the study involved 60 children between 1 month and 4 years of age of whom 30 were treated with virasol and another 30 made a control group. virasol was shown to reduce the severity of the clinical picture to a considerable degree and speed up the recovery in infants with rs virus disease. virasol is recommended for inhalation treatment of infants ... | 1992 | 1441436 |
| bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. analysis of the specificity and activity of serum antibodies. | cattle were immunized with vaccines containing modified-live or inactivated bovine respiratory syncytial virus (brsv) and serum antibody responses were analyzed. compared with preinculation values, at day 14 after two biweekly immunizations with modified-live or inactivated vaccines there were significant increases in brsv-specific titers in the sera of cattle that received both types of vaccines, as determined by a whole cell elisa. using a blocking elisa and radioimmune precipitation it was de ... | 1992 | 1441228 |
| bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. analysis of proliferation and secretion of lymphokines by leukocytes in vitro. | cattle were immunized with vaccines containing modified-live or inactivated bovine respiratory syncytial virus (brsv) and lymphocyte proliferative responses and cytokine secretion were monitored sequentially. compared to pre-inoculated values, significant increases in proliferative responses to modified-live brsv were detectable by day 7 after the primary immunization with the vaccine containing inactivated brsv, and by 7 days after the second immunization with modified-live virus. after a third ... | 1992 | 1441227 |
| the role of viruses and mycoplasma pneumoniae in lower respiratory tract infections in childhood. | acute lower respiratory tract infections are one of the major causes of childhood morbidity and mortality in developing countries. this study was undertaken at hacettepe university children's hospital to determine the role of viruses and mycoplasma pneumoniae in respiratory tract infections in children. eighty-three patients with lower respiratory tract infections were selected at random from among the children admitted to the hospital for evaluation of respiratory symptoms. acute and convalesce ... | 1992 | 1440953 |
| intensive care course and outcome of patients infected with respiratory syncytial virus. | picu admissions of 97 children positive for respiratory syncytial virus on fluorescent antibody screening were reviewed; 68% of 44 patients without history of preceding disease (group i) and 79% of 53 patients with preceding pulmonary, cardiac, or other disease (group ii) required ventilation. in group i ventilated children weighted significantly less (p = 0.001) and were of lower chronological (p = 0.02) and post-conceptional ages (p = 0.02) than those not ventilated. eighteen infants ventilate ... | 1992 | 1437327 |
| pulmonary histopathology induced by respiratory syncytial virus (rsv) challenge of formalin-inactivated rsv-immunized balb/c mice is abrogated by depletion of cd4+ t cells. | in previous studies, it was observed that children immunized with a formalin-inactivated respiratory syncytial virus vaccine (fi-rsv) developed severe pulmonary disease with greater frequency during subsequent natural rsv infection than did controls. during earlier efforts to develop an animal model of this phenomenon, enhanced pulmonary histopathology was observed after intranasal rsv challenge of fi-rsv-immunized cotton rats. progress in understanding the immunologic basis for these observatio ... | 1992 | 1433525 |
| respiratory syncytial virus detection by dot blot hybridization with a nonradioactive synthetic oligo deoxynucleotide probe. | a synthetic oligodeoxynucleotide corresponding to a region of the nucleocapside gene (n) of respiratory syncytial virus (rsv), was used as a dna probe to develop a nonradioactive hybridization assay for the detection of rsv. the probe was labeled by incorporation of biotin-7-datp to the 3' end by a reaction catalyzed by terminal deoxynucleotydil transferase. the dot blot hybridization assay was found to be specific for rsv when tested against rsv isolates (subgroups a and b) obtained from cell c ... | 1992 | 1431818 |
| immunotherapy of respiratory syncytial virus infection in cotton rats (sigmodon fulviventer) using igg in a small-particle aerosol. | to determine whether aerosolized igg can be used effectively in the treatment of respiratory syncytial virus (rsv) infections, cotton rats were infected intranasally with rsv and treated 3 days later with human igg containing anti-rsv antibodies delivered in a small-particle aerosol. pulmonary histology and virus titers were determined 24 h after igg treatment. a single 15-min exposure to aerosolized igg did not exacerbate pulmonary pathology and effected a 50-fold reduction in pulmonary virus t ... | 1992 | 1431258 |
| a novel, spectrophotometric microneutralization assay for respiratory syncytial virus. | we describe a simple and rapid microneutralization assay for respiratory syncytial virus (rsv) based on the colorimetric quantitation of the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) to a formazan product by the mitochondria of viable cells. conditions for rsv infectivity were first optimized for sensitivity and reproducibility based on cell density and on rsv concentration as a function of multiplicity of infection (moi) and time post-infection and the re ... | 1992 | 1430065 |
| direct detection of immunogold reactions by real-time video microscopy. | video-enhanced microscopy allows the detection and tracking of individual colloidal gold particles. the analysis of immunogold reactions can also be conducted as a function of time and thus allows the study of dynamic events in living cells. the direct visualization in real time is reported of the reaction of immunogold particles with a surface antigen. this time-resolved immunocytochemistry was achieved by continuous observation of living cells infected with a virus (respiratory syncytial virus ... | 1992 | 1429013 |
| evaluation of three rapid enzyme immunoassays and cell culture for detection of respiratory syncytial virus. | three rapid enzyme immunoassay techniques for the detection of respiratory syncytial virus antigen (becton dickinson directigen rsv, abbott rsv testpack and abbott rsv eia) and cell culture were evaluated in a total of 250 nasal washings. the sensitivity and specificity were 62% and 76% respectively for directigen, 64% and 86% for rsv testpack, and 76% and 81% for rsv eia, taking cell culture as the reference method. agreement between cell culture and eia techniques was 79% (70 positive and 128 ... | 1992 | 1425717 |
| respiratory syncytial virus infections. | 1992 | 1424399 | |
| field trials on a live bovine respiratory syncytial virus vaccine in calves. | field trials were carried out in calves using a live bovine respiratory syncytial (brs) virus vaccine prepared from the attenuated brs virus, strain rs-52. two hundred seventy-five and 353 calves were vaccinated intranasally and intramuscularly, respectively. no undesirable postvaccinal reactions were observed in the vaccinated calves. of the serum neutralizing (sn) antibody negative calves 89.7% (26/29) and 92.8% (90/97) developed sn antibody 1 month after intranasal and intramuscular vaccinati ... | 1992 | 1420579 |
| detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. | the reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (rsv) sequences in middle ear effusions collected from children with otitis media. sequences of rsv were detected in 21 of 34 samples tested. these samples were collected during and/or after natural outbreaks of rsv infection in the community. in those patients from whose nasopharynges rsv was isolated, the viral sequences were highly detectable (75% ... | 1992 | 1416657 |
| immunologic response of adenoidal lymphocytes to respiratory syncytial virus. | adenoidal lymphocytes obtained from 43 subjects with serum antibodies to respiratory syncytial virus (rsv) were established in culture in vitro and analyzed for immunoglobulin (ig) and rsv-specific antibody synthesis. spontaneous synthesis of ig was consistently observed in culture supernatants. the ratios of iga to igg and igm to igg in adenoidal lymphocyte culture supernatant were higher than in serum. in cell cultures stimulated with rsv or pokeweed mitogen, rsv antibody activity was detected ... | 1992 | 1416640 |
| effects of allergen challenge on plasma concentrations of prostaglandins, thromboxane b2, and histamine in calves infected with bovine respiratory syncytial virus. | to examine the influence of allergen-induced type-1 hypersensitivity on the pathogenesis of bovine respiratory syncytial virus (brsv) infection, we sensitized calves by aerosol to micropolyspora faeni (mf) and challenge exposed them during infection with brsv. the development of mf-specific ige serum concentrations was confirmed by elisa. the dynamics of arachidonic acid metabolism and histamine release during a type-1 hypersensitivity reaction in the bovine lung were studied by quantitating the ... | 1992 | 1416375 |
| analysis of relatedness of subgroup a respiratory syncytial viruses isolated worldwide. | respiratory syncytial virus strains (subgroup a) isolated from around the world during the period 1988-1991 were analysed to determine their relatedness. analysis was by restriction mapping and nucleotide sequencing following amplification of selected regions of the virus genome by polymerase chain reaction (pcr). twenty-three viruses of subgroup a isolated from cities in temperate regions of the northern and southern hemispheres and the tropics during the period 1988-1991 fell into distinct gro ... | 1992 | 1413992 |
| polylactosaminoglycan modification of the respiratory syncytial virus small hydrophobic (sh) protein: a conserved feature among human and bovine respiratory syncytial viruses. | we investigated the nature of the oligosaccharide modification of the glycosylated forms of the small hydrophobic integral membrane protein, sh (previously designated 1a), of respiratory syncytial (rs) virus. analysis of sh protein expressed in cells infected with rs virus or with a recombinant vaccinia virus revealed two glycosylated sh protein species, shg and shp, which contained n-linked carbohydrate residues. shp migrated diffusely on polyacrylamide gels, which suggested modification by pol ... | 1992 | 1413513 |
| risks connected with the use of conventional and genetically engineered vaccines. | a review is given of real and potential risks connected with the use of conventional and genetically engineered live and dead vaccines. special attention is given to live carrier vaccines expressing one or more heterologous genes of other microorganisms. because most carrier vaccines are still in an experimental phase, there is only limited experience with the risks of carrier vaccines. there are three potential risks of live carrier vaccines which will be discussed: 1. changes in cell, tissue, ... | 1992 | 1413441 |
| sero-epidemiological studies of respiratory syncytial and adenoviruses in children in ibadan, nigeria, 1985-1988. | retrospective and prospective serological surveys to determine the prevalence of respiratory syncytial virus (rsv) and adenovirus (adv) infections in children with respiratory diseases were carried out from 1985 to 1988 at the university college hospital, ibadan, nigeria. 306 sera from subjects between 3 months and 12 years old were assayed by complement fixation test for antibodies to both viruses. second samples were available from 42 of the subjects for antibody conversion testing. the rate v ... | 1992 | 1412660 |
| first-year infections after initial hospitalization in low birth weight infants with and without bronchopulmonary dysplasia. | symptoms of infection, specific infections, antibiotic course and hospitalizations were evaluated prospectively by using monthly questionnaires after initial hospitalization in 73 low birth weight (lbw) infants (less than 1751 g) and a subgroup of 19 infants with bronchopulmonary dysplasia (bpd) compared with 63 full-term controls matched for age, sex and home locality. the immunoglobulin g, a, m and e levels of the lbw infants were measured at corrected ages of 1, 2, 4, 6, 8, 10 and 12 months. ... | 1992 | 1411318 |
| respiratory syncytial virus and the use of ribavirin. | 1992 | 1406102 | |
| transcription of human respiratory syncytial virus genome rna in vitro: requirement of cellular factor(s). | extracts made from human respiratory syncytial virus (rsv)-infected hep-2 cells synthesized mrnas encoded by all known viral genes. in contrast, rsv ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected hep-2 cells, demonstrating that a cellular factor(s) has a role in rsv gene expression. quantitation of the individual gene mrnas transcribed in vitro revealed polarity of transcription of the g ... | 1992 | 1404620 |
| respiratory syncytial virus puzzle: clinical features, pathophysiology, treatment, and prevention. | 1992 | 1403380 | |
| sequence comparison between the phosphoprotein mrnas of human and bovine respiratory syncytial viruses identifies a divergent domain in the predicted protein. | the nucleotide and deduced amino acid sequences of the phosphoprotein (p) mrna of bovine respiratory syncytial virus (brsv) strain a51908 have been determined. the p mrna is 860 nucleotides long with a single large open reading frame and the encoded polypeptide is 241 amino acids long. comparison with the corresponding sequences of human respiratory syncytial virus (hrsv) subgroups a and b revealed 72 to 74% identity at the nucleotide level, and 81% at the amino acid level. the p protein contain ... | 1992 | 1402819 |
| comparison of a new commercial enzyme immunoassay for rapid detection of respiratory syncytial virus. | two rapid methods for detection of respiratory syncytial virus in respiratory specimens were compared: direct immunofluorescence assay (dfa) with monoclonal antibody and an enzyme immunoassay (eia) (test-pack rsv). ninety-five nasopharyngeal washings and aspirates from 51 children were examined; the patients were hospitalized during a winter outbreak of rsv infection in the first trimester of 1990. a total of 41.0% and 56.8% of these samples were positive by eia and dfa respectively. considering ... | 1992 | 1396733 |
| respiratory syncytial virus morbidity and mortality estimates in congenital heart disease patients: a recent experience. | to determine recent morbidity and mortality rates from respiratory syncytial virus infection in a pediatric congenital heart disease population. | 1992 | 1395661 |
| an r.s.v.p. to r.s.v. (respiratory syncytial virus): declining mortality rates. | 1992 | 1395656 | |
| epitopes at the proteolytic cleavage sites of hiv-1-gp120 and rsv-f protein share a sequence homology: comparative studies with virus-induced and antipeptide antibodies. | the proteolytic cleavage sites of the human immunodeficiency virus type 1 (hiv-1) envelope glycoprotein precursor gp160 and the fusion protein of respiratory syncytial virus (rsv) show a sequence homology. to study this homology two synthetic peptides corresponding to hiv-1-env-gp160-aa 507-518 (kakrrvvqrekr) and rsv-f2-aa 130-136 (skkrkrr) were synthesized. human serum samples from hiv-positive or rsv-positive collections recognized the appropriate peptide in 90.6 or 37.2% respectively. no cros ... | 1992 | 1385356 |
| characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. | two antigenic sites recognized by neutralizing monoclonal antibodies (mabs) directed against the fusion (f) glycoprotein of human respiratory syncytial virus were mapped on the primary structure of the protein by (i) the identification of amino acid substitutions selected in antibody-escape mutants and (ii) the reactivity of synthetic peptides with mabs. the first site contained several overlapping epitopes which were located within the trypsin-resistant amino-terminal third of the large f1 subu ... | 1992 | 1383404 |
| protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies. | the regions of the fusion protein of respiratory syncytial virus (rsv) that react with neutralizing, fusion-inhibiting and highly protective bovine and murine monoclonal antibodies (mabs) were mapped by two methods: (i) competitive binding assays and (ii) production and analysis of antibody-escape mutants. competitive binding assays with 16 murine and 10 bovine mabs identified 11 antigenic sites on the fusion (f) protein, many of which overlapped extensively, and indicated that cattle, a natural ... | 1992 | 1383403 |
| location of the epitope recognized by monoclonal antibody 63g on the primary structure of human respiratory syncytial virus g glycoprotein and the ability of synthetic peptides containing this epitope to induce neutralizing antibodies. | the location of the epitope recognized by monoclonal antibody (mab) 63g on the primary structure of the human respiratory syncytial virus g glycoprotein was determined by testing the reactivity of synthetic peptides with the mab. the role of individual amino acids in this epitope was determined by using a set of 13-mer peptides containing single residue deletions. residues 204 to 209 were found to be essential for antibody binding. these results are in full agreement with previous sequence data ... | 1992 | 1383397 |
| nosocomial respiratory syncytial virus infection in a newborn nursery. | the nosocomial spread of respiratory syncytial virus (rsv) was studied in a newborn nursery in benin city, nigeria at a time the virus was known to be highly prevalent in the community. nasopharyngeal washings were obtained from babies on admission and, thereafter, every 4 days until discharged. questionnaires were administered to medical personnel with upper respiratory tract infection (urti). rsv was detected by an elisa technique. a total of 56 babies were studied, made up of 33 preterm and 2 ... | 1992 | 1381895 |
| epitope specificities of human serum antibodies reactive with respiratory syncytial virus fusion protein. | respiratory syncytial (rs) virus continues to cause serious human respiratory disease and no prophylactic vaccine is yet available. serum antibodies to rs virus fusion protein (f) that have the appropriate specificities and activities could confer protection against severe rs virus infections. to explore human serum antibody responses to rs virus f we first characterised four epitopes on f and then measured the concentrations of human serum antibodies to these sites for 389 sera. individuals var ... | 1992 | 1379425 |
| assignment of mutant tsn19 (complementation group e) of respiratory syncytial virus to the p protein gene. | the mutation responsible for the temperature-sensitive (ts) phenotype of mutant tsn19 (complementation group e) of respiratory syncytial virus has been located to the p protein gene. viral protein synthesis was completely restricted at 39 degrees c, and the tsn19 p protein did not react with an anti-p monoclonal antibody (mab) (3-5) at 33 degrees c. reversion of temperature sensitivity restored reactivity with mab 3-5. nucleotide sequence determination and in vitro expression of cdna clones of p ... | 1992 | 1378878 |
| intracellular processing of the human respiratory syncytial virus fusion glycoprotein: amino acid substitutions affecting folding, transport and cleavage. | the intracellular processing and transport of the respiratory syncytial virus (rsv) fusion (f) glycoprotein was examined by comparing the maturation and stability of wild-type f, uncleaved mutant f and chimeric f glycoproteins expressed by recombinant vaccinia viruses to that of f protein expressed by rsv. one of the recombinant viruses, vf317, expressed f protein (f317) that was processed like the rsv f glycoprotein. f317 was synthesized initially as f0, the uncleaved glycosylated precursor of ... | 1992 | 1375280 |
| rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification. | a rapid method for detection of respiratory syncytial virus (rsv) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (rt-pcr), has been developed. the rt-pcr assay employs oligonucleotide primers specific for the region of the rsv genome which encodes the f1 subunit of the fusion (f) glycoprotein. other respiratory viruses do not give a positive reaction. the rt-pcr assay was tested on 202 nasopharyngeal aspirates collected f ... | 1992 | 1374080 |
| infectious respiratory syncytial virus (rsv) effectively inhibits the proliferative t cell response to inactivated rsv in vitro. | the effect of respiratory syncytial virus (rsv) on the cellular immune response of human mononuclear cells in vitro was examined. inhibition by rsv of the lymphocyte response to phytohemagglutinin in vitro was confirmed using cells from human umbilical cord blood. in addition, rsv significantly inhibited both the proliferative and t cell colony responses of human mononuclear cells to epstein-barr virus. an rsv-specific cellular immune response was induced in vitro by stimulation of mononuclear c ... | 1992 | 1373752 |
| rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies. | the bartels respiratory virus panel detection kit is an indirect fluorescent-antibody (ifa) method that uses pooled and individual antisera for tissue culture confirmation of seven respiratory viruses. we evaluated these reagents for detecting viral antigen in shell vial cultures and by direct staining of cells from respiratory specimens. the isolation from 254 specimens of respiratory viruses in shell vial cultures compared with standard tube cultures was highly sensitive (94%) and specific (97 ... | 1992 | 1372616 |
| reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo. | we transferred the complementarity determining regions from a murine monoclonal antibody that neutralizes infection by respiratory syncytial virus (rsv) to a human igg1 monoclonal antibody. the resulting reshaped human antibody lost affinity for rsv, but an additional alteration to one of the framework regions restored binding affinity and specificity. this second generation reshaped human monoclonal antibody cross-reacted with all clinical isolates of rsv tested and both prevented disease and c ... | 1991 | 1367535 |
| respiratory syncytial virus and ribavirin: quo vadis? | respiratory syncytial virus is the major respiratory pathogen in infants and young children. every year, this unique and ubiquitous virus accounts for substantial morbidity and occasional mortality in this age group. until recently, therapy for respiratory syncytial virus infections was limited to supportive measures such as hydration, supplemental oxygen, and assisted ventilation. therefore, the licensure of ribavirin in 1986 for treatment of respiratory syncytial virus infections raised hopes ... | 1992 | 1365535 |
| respiratory syncytial virus: babies and antibodies. | 1992 | 1365525 | |
| prospective controlled study of four infection-control procedures to prevent nosocomial infection with respiratory syncytial virus. | to determine the most effective infection control procedure in preventing nosocomial infection with respiratory syncytial virus (rsv), we did a prospective controlled study of four infection-control strategies in four wards in a large paediatric hospital in the west of scotland. all children under two years old admitted to four general wards during three winter rsv epidemics (1989-92) were screened for rsv infection (by nasopharyngeal aspirate and direct immunofluorescence) within 18 hours of ad ... | 1992 | 1357462 |
| nosocomial infection with respiratory syncytial virus. | 1992 | 1357459 | |
| cd4+ t cells clear virus but augment disease in mice infected with respiratory syncytial virus. comparison with the effects of cd8+ t cells. | respiratory syncytial (rs) virus-specific t cell lines were derived from the spleens of balb/c mice primed by intranasal infection with rs virus. the lines were expanded by repeated antigenic stimulation in vitro, and separated into cd4+ and cd8+ t cell-enriched fractions by immunomagnetic adhesion. the effects of passive transfer of these fractions into rs virus infected mice were observed. the most severe immunopathological changes were seen in mice receiving cd4+ cells. transfer of cd4+, cd8+ ... | 1992 | 1351433 |
| ribavirin aerosols and respiratory syncytial virus infection. | 1992 | 1346054 | |
| [detection of respiratory syncytial and adenovirus in nasopharyngeal aspirates: comparison of cellular cultures and immunofluorescence]. | respiratory syncytial and adenovirus are 2 of the most important pathogens for respiratory infection in infancy. we compared the results of cellular cultures and immunofluorescence in the detection of these agents in nasopharyngeal exudates from hospitalized infants. evidence for respiratory syncytial virus was searched in 776 samples. immunofluorescence was much more sensitive, detecting 303 positive results, compared to only 142 for cell culture. compared to immunofluorescence, the sensitivity ... | 1992 | 1340571 |
| serological titers to bovine herpesvirus 1, bovine viral diarrhea virus, parainfluenza 3 virus, bovine respiratory syncytial virus and pasteurella haemolytica in feedlot calves with respiratory disease: associations with bacteriological and pulmonary cytological variables. | acute and convalescent serum samples were taken from 59 calves with signs of respiratory disease (cases) and 60 clinically normal animals (controls) during their first month in the feedlot. sera were analyzed for antibodies to bovine parainfluenza 3 (pi3) virus by hemagglutination inhibition, to bovine viral diarrhea (bvd) virus, bovine respiratory syncytial (brs) virus and bovine herpesvirus 1 (bhv1) by virus neutralization, and to pasteurella haemolytica by indirect agglutination (phia) and cy ... | 1992 | 1335831 |
| studies on the epidemiology of child infections. 3. parainfluenza viruses (types 1-4) and respiratory syncytial virus infections. | a seroepidemiological study on the rate of neutralizing antibodies for parainfluenza viruses (types 1-4) and for the respiratory syncytial virus in 2,514 infants and children between 0 and 15 years, residing in bari and its hinterland was carried out. positive results were very high for both the individual parainfluenza serotypes (81.7% for type 3, 78.0% for type 1, 76.6% for type 4, 71.9% for type 2) and for respiratory syncytial virus (88.5%). the pattern of infections due to respiratory syncy ... | 1992 | 1335108 |
| rapid detection of respiratory viruses using mixtures of monoclonal antibodies on shell vial cultures. | eleven hundred and thirty-three clinical specimens submitted to the laboratory for diagnosis of respiratory virus infections were tested by direct immunofluorescence (dif) for respiratory syncytial virus (rsv), by shell vial culture, and by conventional cell culture. the shell vial cultures were stained with 8 different monoclonal antibodies both 1 day and 3-7 days after inoculation. in order to limit the cost and the workload, mixtures of monoclonal antibodies were used. coverslips with hep-2 c ... | 1992 | 1334129 |
| viral pneumonia in the immunocompromised adult with neoplastic disease: the role of common community respiratory viruses. | until recently, the viruses most often identified in lower respiratory tract infections among immunocompromised adults with neoplastic disease have been the herpesviruses (cytomegalovirus, herpes simplex virus, varicella-zoster virus, human herpesvirus-6) and adenovirus. these viral pneumonias occur with greater frequency and/or severity among these adults than among immunocompetent adults. during the last decade, mounting evidence has suggested that the rna viruses that commonly cause lower res ... | 1992 | 1332171 |
| viral infections. | viral infections may cause serious morbidity as well as death in elderly patients. of the rna viruses, influenza virus is the most important pathogen; the majority of influenza-related deaths occur in older patients. respiratory syncytial virus appears to be gaining increasing importance in elderly persons. herpes zoster or shingles is caused by the dna virus, varicella-zoster virus, and its major morbidity in older patients is postherpetic neuralgia. | 1992 | 1330279 |
| the mannose-specific plant lectins from cymbidium hybrid and epipactis helleborine and the (n-acetylglucosamine)n-specific plant lectin from urtica dioica are potent and selective inhibitors of human immunodeficiency virus and cytomegalovirus replication in vitro. | a series of four mannose(man)-, three n-acetylglucosamine (glcnac)n-, ten n-acetylgalactosamine/galactose(galnac/gal)-, one 5-acetylneuraminic acid (alpha-2,3-gal/galnac)- and one 5-acetylneuroaminic acid(alpha-2,6-gal/gal-nac)-specific plant agglutinins were evaluated for their antiviral activity in vitro. the mannose-specific lectins from the orchid species cymbidium hybrid (ca), epipactis helleborine (eha) and listera ovata (loa) were highly inhibitory to human immunodeficiency virus type 1 ( ... | 1992 | 1329650 |
| treatment of acute pulmonary failure with extracorporeal support: 100% survival in a pediatric population. | since february 1990, five children, aged 10 days to 6.5 years, were treated with extracorporeal lung support at our hospital for acute, unrelenting pulmonary failure. two had viral pneumonia: one with respiratory syncytial virus (rsv) bronchiolitis, and one with herpes simplex virus pneumonia, encephalitis, and disseminated intravascular coagulation. one presented with a febrile illness followed by a pulmonary hemorrhage. two patients had adult respiratory distress syndrome (ards) complicating s ... | 1992 | 1328587 |
| synthesis and antiviral properties of (+/-)-5'-noraristeromycin and related purine carbocyclic nucleosides. a new lead for anti-human cytomegalovirus agent design. | (+/-)-5'-noraristeromycin (3) has been prepared in three steps beginning with the 2,3-o-isopropylidene derivative of (+/-)-(1 alpha, 2 beta, 3 beta, 4 alpha)-4-amino-1,2,3-cyclopentanetriol (7). also prepared from the same starting material were the related hypoxanthine (4), guanine (5), and 2,6-diaminopurine (6) analogues. compounds 3-6 were evaluated for antiviral activity against a large number of viruses with marked activity being observed for 3 towards vaccinia virus, human cytomegalovirus, ... | 1992 | 1326633 |
| activation of human eosinophils in vitro by respiratory syncytial virus. | to determine the nature of the interaction between viruses and eosinophils, normodense eosinophils were separated from the blood of healthy volunteers and incubated in vitro with respiratory syncytial virus (rsv). after incubation for 2 h with the virus, 29.5 +/- 15.8% of the eosinophils demonstrated specific binding of the virus to the cell membrane, as detected by fluorescent staining with an anti-rsv mab. superoxide production and leukotriene c4 release were measured as determinants of cell a ... | 1992 | 1324464 |
| paget's disease of bone--current thinking and management. | the objective of this article was to critically review the current knowledge on paget's disease of bone, focusing primarily on possible etiologies and trends in management. current courses of treatment are described, including calcitonin, diphosphonate and mithramycin therapy and the efficacy of each drug regime is analyzed. finally, newer, more experimental pharmaceutical agents, such as gallium nitrate, are described. | 1992 | 1321871 |
| bovine respiratory syncytial virus fusion protein gene: sequence analysis of cdna and expression using a baculovirus vector. | the nucleotide sequence of bovine respiratory syncytial virus (rsv), atcc strain a51908 fusion (f) glycoprotein gene cdna was determined. the amino acid sequence deduced was then compared to those of two different isolates of bovine rsv, strains rb 94 and 391-2, and the a and b subtypes of human rsv, strains 18537 and a2. the bovine rsv f protein is highly conserved between the three isolates, a51908 has 97% amino acid identity to rb 94, and 99% identity to 391-2. the f proteins of both the a an ... | 1992 | 1318950 |
| protein of macaques against infection with simian type d retrovirus (srv-1) by immunization with recombinant vaccinia virus expressing the envelope glycoproteins of either srv-1 or mason-pfizer monkey virus (srv-3). | rhesus macaques were immunized with live vaccinia virus recombinants expressing the envelope glycoproteins (gp70 and gp22) of simian type d retrovirus (srv), serotype 1 or 3. all of the animals immunized with either the srv-1 env or the srv-3 env vaccinia virus recombinant developed neutralizing antibodies against the homologous srv. in addition, both groups developed cross-reactive antibodies and were protected against an intravenous live-virus challenge with srv-1. the four control animals imm ... | 1992 | 1316495 |
| effect of macrophages and in vitro infection with parainfluenza type 3 and respiratory syncytial viruses on the mitogenic response of bovine lymphocytes. | bovine blood lymphocytes, depleted of macrophages by absorption on plasma-gelatin coated plastic flasks, followed by passage through sephadex g-10 columns, failed to respond to pokeweed mitogen stimulation. adherent monocytes or alveolar macrophages added to purified lymphocyte preparations at 10% or less were able to restore the transformation response. exposure of alveolar macrophages or purified lymphocytes to 2 bovine respiratory syncytial virus strains for 24 hours substantially reduced the ... | 1992 | 1315492 |
| epidemiology and aetiology of acute bronchiolitis in hong kong infants. | the epidemiological, clinical and virological features of 1220 children with acute bronchiolitis admitted to the prince of wales hospital, hong kong, from 1985 to 1988 are reported. they accounted for 6.6% of total paediatric admissions and provided a case incidence of bronchiolitis requiring admission to hospital of approximately 21 per 1000 children 0-24 months of age. the clinical course and outcome was in general benign. the average hospital stay was 5 days and there were no deaths. ten per ... | 1992 | 1312477 |
| release of tumor necrosis factor-alpha from bovine alveolar macrophages stimulated with bovine respiratory viruses and bacterial endotoxins. | the release of tumor necrosis factor-alpha (tnf-alpha) from cultured bovine alveolar macrophages (bam) was evaluated following stimulation of bam with bovine herpesvirus-1 (bhv-1), parainfluenza-3 (pi-3) virus, bovine respiratory syncytial virus (brsv), escherichia coli 0111:b4 endotoxin, pasteurella haemolytica type 1 endotoxin, pasteurella multocida endotoxin, and virus/endotoxin combinations. a cytotoxic assay system using georgia bovine kidney cells as targets was used to measure tnf-alpha a ... | 1992 | 1312264 |
| sequence analysis of m2 mrna of bovine respiratory syncytial virus obtained from an f-m2 dicistronic mrna suggests structural homology with that of human respiratory syncytial virus. | the nucleotide sequences of the f and m2 mrnas of strain a51908 of bovine respiratory syncytial virus (brsv) were determined by sequencing cdna of an intracellular dicistronic mrna. comparison of the f mrna sequence with those of other brsv strains showed that there was extensive sequence identity at both the nucleotide (95% identity) and amino acid (94% identity) levels. alignment of the nucleotide and encoded amino acid sequences of m2 mrna of brsv with those of human respiratory syncytial vir ... | 1992 | 1312130 |
| interaction of respiratory syncytial virus with human cord blood mononuclear cells. | this study on the interaction between respiratory syncytial virus (rsv) and human cord blood mononuclear cells shows that rsv replication can occur in neonatal macrophages. although neonatal lymphocytes were not supportive of rsv replication, exposure to rsv resulted in significant inhibition of mitogen-induced transformation. both adult and neonatal nk cell cytotoxicity were unaffected by exposure to rsv. these results suggest that rsv has preferential effects on human cord blood mononuclear ce ... | 1992 | 1305815 |
| [clinical and experimental study of xiao er ke cuan ling oral liquid in the treatment of infantile bronchopneumonia]. | xiao er ke chuan ling oral liquid (kcl) is a chinese herbal preparation consisted of 10 herbs such as prunus armeniacae, scutelaria baicalensis, lonicera japonica etc. 30 children suffering from bronchopneumonia and/or acute bronchitis were treated with kcl (treated group) and another 30 cases were treated with penicillin and aminophylline (control group). results: cure rate and effective rate in treated group was 26.6%, and 93.3% respectively. while in control group was 30% and 96.6% respective ... | 1992 | 1304839 |
| [ezhu intravenous injection in treating infantile respiratory syncytial virus pneumonia and its therapeutical mechanism]. | ezhu intravenous injection (eii) in treating respiratory syncytial virus (rsv) pneumonia was studied. 45 patients in therapeutic group were treated with 0.04% eii in dose of 20 ml/kg/day for 7-14 days. 20 patients in the control group were treated with huayu decoction, its efficacy on rsv pneumonia has been confirmed. the results showed that all the patients of both groups were cured and there were no statistic differences in the average days of clearing up the temperature, dyspnea and wheezing ... | 1992 | 1304837 |
| [clinical manifestation and prognosis of respiratory syncytial virus infection in infants]. | to evaluate the clinical features of respiratory syncytial virus (rsv) infection and relation with bronchial asthma, 233 infants hospitalized for lower respiratory tract infection during winters from 1988 to 1990 were enrolled in this study. rsv antigen was detected from half of them by immunofluorescent antibody test. children with rsv infection showed the characteristic findings as follows; wheeze (71.6%), elevated serum 2-5 as activity, a marker for viral infection, low level of acute phase r ... | 1992 | 1290411 |
| [influenza a infection in children. clinical spectrum and comparison with respiratory syncytial virus infection during the winter 1989-1990]. | respiratory syncytial viral (rsv) infection can be rapidly differentiated from influenza viral infection by immunofluorescence techniques. these tests were used to identify some epidemiological and clinical characteristics of both infections. | 1992 | 1288452 |
| human monoclonal fab fragments derived from a combinatorial library bind to respiratory syncytial virus f glycoprotein and neutralize infectivity. | respiratory syncytial virus (rsv) is the most important cause, throughout the world, of severe viral lower respiratory tract illness in young children. antibodies are known to mediate resistance to rsv infection and illness. we have isolated a number of human monoclonal fab fragments to rsv f glycoprotein from a combinatorial antibody library expressed on the surface of phage. one of these neutralized a wide range of virus isolates, 10 subgroup a and 9 subgroup b isolates, with a titer (60% neut ... | 1992 | 1279672 |
| persistent infection of cells in culture by respiratory syncytial virus. | the virus-cell relationship of rs virus and the hep-2 cell line has been examined. the production of cytopathic effect (c.p.e.) on hep-2 cells has been found to be dependent upon the passage level of the cell line. cells at lower passage levels exhibit c.p.e. in the form of syncytium formation, while those at higher passage levels no longer exhibit this effect. cells infected at higher passage levels are covertly infected and continue to produce large amounts of infectious virus which remains c ... | 1976 | 1265052 |
| the pathogenesis of respiratory syncytial virus infection in infant ferrets. | the infant ferret is susceptible to respiratory syncytial virus infection in both the upper and lower respiratory tracts. in the nose, viral replication is restricted to the surface respiratory epithelium in the nasal passages and turbinates. in the lungs, viral replication is of a lower order of magnitude and is localized in the alveolar cells. the pattern of viral replication in nasal tissues is independent of the age of the animal at infection, whereas the pattern in lung tissues shows a stri ... | 1976 | 1251889 |
| cell-mediated immunity to respiratory syncytial virus induced by inactivated vaccine or by infection. | transformation and increased mitotic activity in donor lymphocytes exposed to specific antigens is considered by many to be a manifestation of cell-mediated immunity. in attempts to understand the apparent "sensitization" of individuals to respiratory syncytial virus (rsv) as a result of receiving inactivated rsv vaccine, in vitro lymphocyte transformation studies were carried out on infants who had received inactivated rsv vaccine and on infants who had received a similarly prepared inactivated ... | 1976 | 1246465 |
| [viremia in respiratory syncytial virus infection]. | viremia was demonstrated to occur in experimental respiratory syncytial (rs) virus infection in suckling cotton rats and in natural infection in children. rs virus was isolated from the whole blood of the animals in 3 out of 6 experiments at 2, 5, 6, 7 and 15 days after inoculation, the maximum infectious titer being more than 10(4) tcpd50/0.1 ml. rs virus was also isolated from the blood of 7 out of 15 examined children presenting the typical clinical picture of rs virus disease during the epid ... | 1975 | 1216838 |
| [frequency of serologic conversion of complement fixing antibodies fc by respiratory viruses in cases of acute juvenile respiratory disease]. | the complement fixation test, run on double samples of serum pertaining to 447 children hospitalized between january 1971 and june 1974 at ospedale maggiore in novara, or checked in consulting rooms for acute phlogosis of the respiratory organs, has permitted to detect in 241 children, equal to the 53,9% of the total cases, a significant increase of antibodies for respiratory viruses (influenza a, b, c, parainfluenza 1, 2, 3. respiratory syncytial virus, adenovirus, reovirus). specifically, in t ... | 1975 | 1205032 |
| nosocomial respiratory syncytial virus infections. | we studied the frequency and severity of respiratory syncytial virus infections acquired nosocomially on an infants' ward during a community outbreak. every three or four days all infants and staff were examined, and specimens were obtained for viral isolation. during two months, 14 of 44 contact infants acquired the virus. all were ill, and four had pneumonia. infected infants had a significantly longer mean hospital stay (21.5 days) than uninfected ones (9.2 days, p less than 0.001). risk of n ... | 1975 | 1186836 |
| stability of respiratory syncytial virus in a new bentonite holding medium. | stability of respiratory syncytial virus was determined in a new bentonite holding medium and compared with two other commonly used media. eventual infectivity titers were similar with all three preparations. | 1975 | 1176622 |
| reye syndrome with associated influenza a and b infection. | in early 1974, seventeen children were treated for reye syndrome. thirteen of these were studied for laboratory evidence of concomitant viral infection. influenza b/hong kong was isolated from the pharynx in four of nine 1974 cases tested. one child had just recovered from varicella. adenovirus type 2 and respiratory syncytial virus were isolated from two additional patients. serologic tests for influenza suggested concomitant or recent influenza b infection in ten of 13 of 1971 cases. during fe ... | 1975 | 1174373 |
| viral antibodies in the csf after acute cns infections. | viral antibodies were measured in the cerebrospinal fluid (csf) and serum from 25 patients having acute viral central nervous system (cns) infections, and from 39 control patients. the results, collected two weeks after the clinical onset, revealed the presence of antibodies in nine of 13 (69%) csf specimens from patients suffering from encephalitis of myelitis, and in only one of nine (11%) of the csf samples of those presenting a viral meningitis infection. this difference was statistically si ... | 1975 | 1164214 |
| [formation and action of interferon in experimental respiratory syncytial infection]. | respiratory syncytial virus multiplying activity in the lungs of newborn and 6-day-old cotton rats, induced production of considerable amounts of interferon. interferon production correlated with virus multiplication. lower interferon titers were observed in the lungs of newborn cotton rats than in the lungs of 6-day-old animals. rs virus also induced production of serum interferon upon intraperitoneal inoculation, its titers being 2 times as low as after inoculation of equal doses of newcastle ... | 1975 | 1162951 |
| hospital cross-infection on children's wards with respiratory syncytial virus and the role of adult carriage. | a further 18 children who acquired respiratory syncytial (r.s.) virus infection on general paediatric wards have been identified, bringing the total number of hospital cross-infections by this virus to 46 for the last 3 annual r.s. virus epidemics on tyneside. 25 of these 46 illnesses involved the lower respiratory tract. carriage of r.s. virus by staff and visitors was studied on one ward, and appeared to be responsible for at least 2 and probably 4 of the 6 cross-infections detected on this wa ... | 1975 | 1155072 |
| isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus. | the isolation and characterization of a bovine respiratory syncytial (rs) virus is described. serological studies indicate that bovine rs virus is widespread in iowa cattle and that it is involved in some outbreaks of respiratory disease. experimental infection in calves indicates that the virus can cause illness in calves, particularly those with serum neutralizing antibody. | 1975 | 1119944 |