Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunoprophylaxis of infections with respiratory syncytial virus: observations and hypothesis. | infections with respiratory syncytial virus (rsv) may cause substantial morbidity and increased mortality in neonates and infants, especially those born before term and/or with pulmonary or cardiac disease. humoral immunity was long thought to play only a small role in prevention, reduction of severity, or recovery from rsv infections of the lower respiratory tract. we recently performed a series of experiments in rsv-infected cotton rats, owl monkeys, and human infants to assess the role of hum ... | 1990 | 2194271 |
| evaluation of five methods for respiratory syncytial virus detection. | a total of 117 nasal aspirates were cultured for respiratory syncytial virus (rsv) and tested for rsv antigen by a direct fluorescent-antibody (dfa) test (bartels immunodiagnostic supplies, inc., bellevue, wash.), the directigen enzyme immunoassay (eia; becton dickinson microbiology systems, cockeysville, md.), the testpack eia (abbott laboratories, north chicago, ill.), and rsv eia (abbott). agreement of two of five methods or a positive rsv culture were required to validate a result. a total o ... | 1990 | 2191003 |
| [laboratory diagnosis of respiratory syncytial virus infection]. | we study 18 children with acute respiratory infection diagnosed early by direct immunofluorescence (dif) using monoclonal antibodies to synticial respiratory virus (srv). antibodies to adenovirus, influenza virus a and b, parainfluenza virus 1, 2 and 3, mycoplasma, q fever, herpes and cytomegalovirus (cmv) are also studied. we consider that in most cases the diagnosis of infections caused by srv cannot be established by complement fixation, and should be accompanied by some antigen detection tec ... | 1990 | 2189329 |
| mechanism of antibody-mediated viral clearance in immunotherapy of respiratory syncytial virus infection of cotton rats. | antibody-mediated clearance of respiratory syncytial virus from cotton rat pulmonary tissues occurs in the absence of complement and in the absence of the fc portion of the immunoglobulin g molecule, suggesting that complement-independent, cell-independent neutralization is the major mechanism of clearance. | 1990 | 2186179 |
| rapid detection of respiratory syncytial virus in nasopharyngeal specimens obtained with the rhinoprobe scraper. | we compared the rhinoprobe scraping technique for collection of superficial nasal mucosa epithelial cells and rapid detection of respiratory syncytial virus by immunofluorescence with paired, swab-collected specimens for virus culture from 1,257 infants and children with acute respiratory infections. compared with viral culture as the reference test, the sensitivity, specificity, and accuracy of the immunofluorescence test were 83.6, 93.6, and 91.3%, respectively. we found the rhinoprobe method ... | 1990 | 2185261 |
| comparison of directigen rsv with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus. | an enzyme immunoassay membrane test (directigen rsv) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (if). a total of 315 nasopharyngeal wash specimens from pediatric patients were examined. directigen rsv was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or if, with 88.6% agreement. the false-positive rate was 16%; 2 of 20 specimens giving f ... | 1990 | 2182668 |
| an epidemic of respiratory syncytial virus in elderly people: clinical and serological findings. | in 1984-1985, an outbreak of respiratory syncytial virus (rsv) infection occurred in two geriatric wards. among 68 patients (mean age +/- sd = 82.5 +/- 12.5 with respiratory signs, 52 had signs caused by rsv infection. among all patients, the clinical and serological attack rates were 61.2% and 75.0%, respectively. the most frequent clinical presentation was intensive coughing (96.1%) and fever (96.1%) associated with expectorate (63.5%). the duration of the respiratory symptoms was 5 to 7 days. ... | 1990 | 2179469 |
| viral pneumonia in the first month of life. | we performed a 5-year review of 40 patients less than or equal to 30 days of age with viral pneumonia. isolates included respiratory syncytial virus (55%), enteroviruses (15%), rhinoviruses (15%), adenoviruses (10%), parainfluenza virus (7.5%) and herpes simplex virus (5%). most infants were previously healthy but had ill family members. nine were born at less than 37 weeks of gestation. symptoms and signs included tachypnea, decreased feeding, cough, cyanosis, lethargy, retractions, apnea, brad ... | 1990 | 2177540 |
| a hospital-based study of acute viral infections of the respiratory tract in thai children, with emphasis on laboratory diagnosis. | the hospital-based study described here examined the viruses found in 738 children less than 5 years old who presented at ramathibodi hospital, bangkok, thailand, from january 1986 to december 1987 with acute respiratory tract infections. three methods for detection of viral infection are compared: direct examination of epithelial cells of the respiratory tract with the use of fluorescent antibody staining, isolation of virus, and measurement of antibody in acute- and convalescent-phase sera. vi ... | 1990 | 2176738 |
| acute lower respiratory tract infection due to virus among hospitalized children in dhaka, bangladesh. | the nature of acute lower respiratory tract infection (alri) in hospitalized children and of the associated viral agents was assessed in a study of 601 children less than 5 years old over a 24-month period. of these children, 80% were less than 24 months of age and the ratio of boys to girls was 1.7:1. pneumonia (86.5% of cases) was the most frequently observed clinical manifestation. shedding of virus was detected in 21.1% of the children; the highest rate occurred in infants 0-5 months old (27 ... | 1990 | 2176737 |
| isolation and identification of viral agents in argentinian children with acute lower respiratory tract infection. | from a total population of 1,002 children with acute lower respiratory infection (alri), identification of virus was achieved in 304 cases. in this survey, 1,000 nasopharyngeal aspirate and 13 lung tissue samples were tested in four cell lines (hep-2, mrc-5, mdck, and llc-mk2) and by indirect immunofluorescence (iif), while 242 paired sera were studied by complement fixation. respiratory syncytial virus (rsv) was the most frequently detected agent (n = 183), followed by adenovirus (n = 28), para ... | 1990 | 2176736 |
| clinical role of respiratory virus infection in acute otitis media. | the clinical characteristics of acute otitis media in relation to coexisting respiratory virus infection were studied in a 1-year prospective study of 363 children with acute otitis media. respiratory viruses were detected using virus isolation and virus antigen detection in nasopharyngeal specimens of 42% of the patients at the time of diagnosis. rhinovirus (24%) and respiratory syncytial virus (13%) were the two most common viruses detected. adenovirus, parainfluenza viruses, and coronavirus o ... | 1990 | 2174535 |
| primate viral diseases in perspective. | the recent occurrence of fatal herpesvirus simiae (b virus) infection in human subjects has again focused the attention of primatologists on this virus. b virus, however, is only one of a number of viral diseases that plays a role in primate colony management. this report is to emphasize to the primatologist a number of viruses other than h. simiae, with high morbidity and mortality rates, of importance for health management of nonhuman primate animal colonies. this concept is supported by the r ... | 1990 | 2174083 |
| pulmonary lesions induced by 3-methylindole and bovine respiratory syncytial virus in calves. | our objectives were to describe the ultrastructural morphogenesis of pulmonary lesions induced by 3-methylindole in 30- to 45-day-old holstein calves and to determine whether toxic exposure to 3-methylindole exacerbates pulmonary lesions induced by bovine respiratory syncytial virus. administration of 3-methylindole (0.25 g/kg) to calves resulted in interstitial edema and ultrastructural swelling of type-i alveolar epithelial cells and nonciliated bronchiolar epithelial cells as early as 4 to 6 ... | 1990 | 2173450 |
| respiratory syncytial virus and parainfluenza virus surveillance--united states, 1989-90. | 1990 | 2172757 | |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| o glycosylation of glycoprotein g of human respiratory syncytial virus is specified within the divergent ectodomain. | cdnas encoding the g glycoprotein of respiratory syncytial virus and the hemagglutinin-neuraminidase (hn) glycoprotein of parainfluenza virus type 3 were modified by site-specific mutagenesis and restriction fragment replacement to encode chimeric proteins consisting of the cytoplasmic and transmembrane domains of one protein fused to the ectodomain of the other. in the case of the hn ectodomain attached to the g transmembrane and cytoplasmic domains, cell surface expression of the chimera was r ... | 1990 | 2164608 |
| new acquisitions in the chemotherapy of viral infections. | the development of new antiviral agents has gained increasing momentum. it has kept pace with the identification of specific sites ("targets") in the virus replicative cycle at which potential antiviral drug can interact. the current armamentarium of available antiviral drugs consists of amantadine and rimantadine (against influenza a), ribavirin (against respiratory syncytial virus infection), idoxuridine and trifluridine (against herpetic keratitis), vidarabine and acyclovir (against herpes si ... | 1990 | 2162118 |
| viral pneumonia in recipients of solid organ transplants. | viral pulmonary infections are a major cause of morbidity and mortality in solid organ transplant recipients. the herpes viruses-cytomegalovirus, herpes simplex virus, varicella zoster virus, and epstein-barr virus--cause most of the viral infections in this population. respiratory syncytial virus, adenovirus, and human immunodeficiency virus also cause pneumonitis in the transplant recipient. differences in the clinical and laboratory presentation of pneumonitis due to the various viral agents ... | 1990 | 2160718 |
| surveillance of viral respiratory tract infections over a one year period in mainly hospitalized austrian infants and children by a rapid enzyme-linked immunosorbent assay diagnosis. | in order to obtain more information on viral respiratory tract infections in austrian infants and children, nasopharyngeal secretions from 1432 infants and children, collected from october 1984 to october 1985, were screened for the presence of respiratory syncytial virus (rsv), adenoviruses, parainfluenza virus type 1, 2, and 3, and influenza viruses type a and b, by enzyme-linked immunosorbent assay (elisa). the results obtained were analyzed with respect to incidence, seasonal distribution an ... | 1990 | 2156381 |
| viral and toxoplasma gondii infections in children after liver transplantation. | the incidence and morbidity of viral and toxoplasma gondii infections were studied in 40 children who underwent liver transplantation between december 1983 and february 1988. the incidence of primary and reactivated cytomegalovirus (cmv) infection was 19% and 47%, respectively; primary infection caused clinical disease in all five cases affected and was fatal in one. primary epstein-barr virus (ebv) infection occurred in 10 (26%) recipients but caused only mild disease. no reactivated ebv infect ... | 1990 | 2155947 |
| outbreak of parainfluenza virus type 3 in a neonatal nursery. | human parainfluenza type 3 is the most common cause of bronchiolitis and pneumonia after respiratory syncytial virus. in a recent outbreak of nosocomial respiratory illness in a neonatal intensive care unit, parainfluenza type 3 virus was isolated in 6 of 17 neonates cultured (5 symptomatic patients and 1 asymptomatic patient). eighteen of 52 nursing personnel had been ill during the previous week and concomitantly, with cough and nasal congestion. these personnel and all patient care givers wer ... | 1990 | 2153949 |
| viral lower respiratory tract infections in filipino children. | viral causes of acute lower respiratory tract infection were studied prospectively between 15 june and 31 october 1984 in 312 filipino children less than 5 years old living in periurban slums and middle-class housing. the cause was based on viral antigen detection, virus isolation, and antibody assays. there were 131 children (41.2%) who were admitted to the hospital, and 150 (47%) had an infiltrate on chest radiograph. a total of 198 viral infections were confirmed in 162 patients (51.9%), 42.3 ... | 1990 | 2153734 |
| respiratory syncytial virus: occurrence of subgroups a and b strains in rio de janeiro. | 1990 | 2152204 | |
| [the clinico-epidemiological characteristics of the course of a mixed epidemic of influenza and respiratory syncytial infection in novoshakhtinsk in the summer of 1986]. | in the summer of 1986 the epidemic, whose etiological agents were influenza viruses a (h1n1) and respiratory syncytial virus, was registered among the population of novoshakhtinsk. in a number of mines 15.3-16.7% of the employees were affected. influenza viruses a (h1n1) proved to be closely related in their antigenic and biological properties to viruses isolated in the ussr in march-june 1986, as well as to viruses a (h1n1), the etiological agents of the epidemic which developed in the ussr in ... | 1990 | 2151553 |
| [immunodiagnostic methods in interpreting an outbreak of respiratory syncytial virus infection]. | in the determination of the etiology of the outbreak of respiratory viral diseases, caused mainly by respiratory syncytial (rs) virus, on the basis of the comparison of the results of different laboratory tests and some epidemiological and clinical data high specificity of the detection of rs virus antigen and antibodies to it by means of erythrocyte diagnostica (newly developed antibody and commercial antigenic preparations) has been revealed. in the epidemiological situations the rapid methods ... | 1990 | 2151499 |
| lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus. | the lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (rsv) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. experimental infection with bovine rsv was characterized by a significant rise in sbu-t8+ (cd8+ or cytotoxic) t cells and a significant reduction in sbu-t4+ (cd4+ or helper) t cells and b (lca p220+) lymphocytes (p less than 0.05). the helper/s ... | 1990 | 2140217 |
| effective inhibition of viral reproduction by hydrophobised antiviral antibodies. | a method is proposed for the inhibition of viral reproduction in cells by means of fatty-acylated antiviral antibodies which, in contrast to the unmodified antibodies, have the ability to enter the cells. the potential of this technique is demonstrated in experiments involving inhibition of the reproduction of various strains of influenza virus and respiratory syncytial virus. | 1990 | 2130920 |
| respiratory syncytial virus modulation of adult and neonatal lymphocyte mitogenic responses and the role of interferon-gamma. | infection of human adult and neonatal mononuclear leukocytes (mnls) with respiratory syncytial virus (rsv) caused significant inhibition of lymphoproliferation in response to various mitogens (heat-killed staphylococcus aureus, phytohaemagglutinin, concanavalin-a and pokeweed mitogen). antibody to human interferon-gamma enhanced the rsv-induced suppression of lymphoproliferation in adult, but not neonatal mnls. these results are consistent with the hypothesis that rsv can suppress the developmen ... | 1990 | 2129048 |
| a study of nonbacterial agents of acute lower respiratory tract infection in thai children. | from january 1986 to december 1987, 596 children less than 5 years of age with lower respiratory tract infection (lri)--manifested as laryngitis, croup, bronchitis, bronchiolitis, and pneumonia--were studied for evidence of infection with respiratory tract viruses mycoplasma pneumoniae, and chlamydia trachomatis. of the 596 children in the study, 315 were ambulatory and 281 were hospitalized. virologic studies included isolation and rapid diagnosis of virus from specimens of nasopharyngeal aspir ... | 1990 | 2125359 |
| excessive secretion of antidiuretic hormone in infections with respiratory syncytial virus. | the association between infections with respiratory syncytial virus and plasma concentrations of antidiuretic hormone was assessed in 48 patients who had been admitted to hospital. the mean (sem) concentration of antidiuretic hormone was significantly raised in patients with bronchiolitis (9.3 (1.4) ng/l) compared with non-pulmonary respiratory syncytial virus infections that cause apnoea or upper respiratory tract symptoms (6.1 (1.7) ng/l). the highest concentrations of antidiuretic hormone wer ... | 1990 | 2123382 |
| respiratory syncytial virus infection. a brief review of epidemiology and current research in china. | 1990 | 2119965 | |
| differentiation of respiratory syncytial virus subgroups with cdna probes in a nucleic acid hybridization assay. | a new approach to respiratory syncytial (rs) virus subgroup determination was developed by using a simple nucleic acid filter hybridization technique. by this method, virus-infected cells are bound and fixed in a single step, and the viral rna in the fixed-cell preparation is characterized directly by its ability to hybridize to cdna probes specific for either the a or b subgroups of rs virus. the subgroup-specific probes were constructed from cdna clones that corresponded to a portion of the ex ... | 1990 | 2118548 |
| economic and long-term benefits of ribavirin therapy on respiratory syncytial virus infection. | ribavirin is a broad-spectrum antiviral agent. administered as an aerosol, it has been shown to be clinically effective in improving the signs and symptoms of viral bronchiolitis in infancy, particularly cases due to respiratory syncytial virus (rsv). this paper reviews the evidence for economic and/or long-term clinical benefits from using ribavirin in the acute illness. there are data to suggest that use of ribavirin may lead to a reduction in therapeutic interventions and duration of hospital ... | 1990 | 2117144 |
| acute and long-term effects of viral bronchiolitis in infancy. | about 1% of infants are admitted to hospital with acute bronchiolitis; 85% of cases are caused by infection with respiratory syncytial virus (rsv). the pathophysiological changes during the acute illness are inflammatory obstruction in the small airways with submucosal cellular infiltration, epithelial necrosis and mucous plugging; frc increases and dynamic compliance falls. failure to respond to bronchodilator drugs suggests that muscle spasm contributes relatively little to the airway narrowin ... | 1990 | 2117143 |
| cytolytic t-lymphocyte responses to respiratory syncytial virus: effector cell phenotype and target proteins. | cytolytic t-lymphocyte (ctl) activity specific for respiratory syncytial (rs) virus was investigated after intranasal infection of mice with rs virus, after intraperitoneal infection of mice with a recombinant vaccinia virus expressing the f glycoprotein, and after intramuscular vaccination of mice with formalin-inactivated rs virus or a chimeric glycoprotein, fg, expressed from a recombinant baculovirus. spleen cell cultures from mice previously infected with live rs virus or the f-protein reco ... | 1990 | 2117070 |
| variation in severity of respiratory syncytial virus infections with subtype. | two major subtypes of respiratory syncytial virus have been identified. this study assessed the hypothesis that a-subtype infections were more severe than b-subtype infections among the 157 infants hospitalized in two hospitals in rochester, n.y., during two winters. severity was measured both by specific clinical observations and by a severity index that was derived empirically. among all subjects, several clinical observations suggested that a-subtype infections were more severe. for example, ... | 1990 | 2115082 |
| targeted immunoglobulin therapy for the prevention of neonatal infections. | premature infants demonstrate hypoglobulinemia and are at increased risk for serious infections. although a cause-and-effect relation between low serum igg levels and neonatal infections has not been established, prophylaxis of such severe infections may be possible by replacement of antibody with intravenous immunoglobulin (ivig). for success, ivig must provide specific antibodies to neonatal pathogens and reach therapeutic serum igg target levels. pilot investigations have demonstrated that iv ... | 1990 | 2114035 |
| [pneumonia in adults caused by the respiratory syncytial virus]. | respiratory syncytial virus (rsv) usually affects children but sometimes affects a certain group of adults. a case of an 81 year old female with idiopathic thrombocytopenic purpura who had pneumonia caused by rsv is presented. the literature is reviewed as well as the most frequent clinical manifestations, diagnosis methods and treatment. | 1990 | 2103289 |
| [clinical diagnosis and laboratory study of acute respiratory diseases of viral origin in children]. | the authors analyzed retrospectively a group of 477 children aged two months to 16 years (mean age 6 years and 5 months), hospitalized on account of acute respiratory disease and subjected to virological examination. the aetiology was revealed in 53% of the sick children. in the elucidation of the aetiology participated a number of respiratory viruses, in particular the respiratory syncytial virus. the clinical picture of the disease was very varied. it was confirmed that the clinical picture do ... | 1990 | 2101749 |
| [evaluation of methods for the detection of syncytial respiratory virus in nasopharyngeal secretions]. | the screening for respiratory syncytial virus (rsv) in nasopharyngeal secretions with enzyme immunoassay (elisa) and indirect immunofluorescence (iif) has been evaluated in infants and young children with acute respiratory infection. both methods were compared with viral isolation in hep-2 cells and the investigation of fluorescent foci in cell cultures inoculated by centrifugation. 226 samples were evaluated by iff, 182 of which were also evaluated by elisa while 158 were inoculated into cell c ... | 1990 | 2098144 |
| comparison of lung histopathology and bronchoalveolar lavage cytology in mice and cotton rats infected with respiratory syncytial virus. | lung histology as well as cell number and size distribution in bronchoalveolar lavage (bal) were compared in balb/c mice and in cotton rats, of various immune status regarding the respiratory syncytial virus (rsv), when subjected to challenge with rsv. in mock-immunized animals, rsv infection typically caused microscopic inflammatory lesions of the lungs and the presence of inflammatory cells in the bal. immunization with a formalin inactivated vaccine prior to challenge increased the severity o ... | 1990 | 2087875 |
| the efficacy of intranasal interferon alpha-2a in respiratory syncytial virus infection in volunteers. | in a double-blind, placebo-controlled study, self-administered intranasal interferon alpha-2a or placebo was given both before and after challenge with respiratory syncytial virus. the incidence of colds and the severity of signs and symptoms were reduced in those receiving interferon alpha-2a as compared with those given placebo. in a further double-blind, placebo-controlled study, self-administered interferon alpha-2a or placebo was given only to those volunteers who developed colds following ... | 1990 | 2080867 |
| increased susceptibility to pasteurella haemolytica in lambs infected with bovine respiratory syncytial virus. | groups of conventionally reared, 6- to 8-week-old lambs were inoculated intranasally and intratracheally with either bovine respiratory syncytial virus (brsv) or brsv followed by pasteurella haemolytica 6 days later or with p. haemolytica alone. lambs infected with p. haemolytica 6 days after experimental infection with brsv had significantly higher disease scores, greater magnitudes of fever, and higher mortality rates than those infected with brsv alone or with p. haemolytica alone (p less tha ... | 1990 | 2079556 |
| [seroepidemiologic studies of the distribution of respiratory syncytial virus in wild ruminants]. | sera from wild ruminants (302 roe, 39 red deer, 17 fallow deer, 1 mouflon) were tested for antibodies against respiratory syncytial virus (rsv). 22 sera (6.1%) were positive: 20 roe, 1 red deer, 1 mouflon. the findings are discussed from the epizootiological point of view. | 1990 | 2078187 |
| primary pulmonary murine cytotoxic t lymphocyte specificity in respiratory syncytial virus pneumonia. | effector cells capable of lysing respiratory syncytial virus (rsv)-infected cells were isolated from the lungs of intranasally infected mice. an examination of their specificity showed that cytolysis was major histocompatibility complex restricted. using recombinant vaccinia viruses containing cloned rsv genes to infect target cells, balb/c (h-2d) pulmonary effector cells were shown to recognize the fusion protein (f) and to a lesser extent the nucleoprotein (n). cells specific for the major gly ... | 1990 | 2077342 |
| [ultrastructural changes in middle ear and cochlea caused by respiratory syncytial virus in guinea pigs]. | respiratory syncytial virus (rsv) was inoculated into the middle ear of guinea pigs. preyer's reflex began to decrease since 21st day. the positive rate of rsv culture from middle ear mucosa within the first week was 50%. the titer of igg antibody against rsv in sera rose gradually. electron microscopy showed enlarged rough endoplasmic reticulum, swelling mitochondria and pyknosis of nuclei in the epithelial and mononuclear cells of middle ear. the surface of corti's organ was covered with exuda ... | 1990 | 2076325 |
| respiratory syncytial virus infection in c57bl/6 mice: clearance of virus from the lungs with virus-specific cytotoxic t cells. | we describe respiratory syncytial virus (rsv)-specific cytotoxic t-cell (ctl) lines and clones developed from the spleens of c57bl/6 and balb/c mice. line 7 and clones derived from it were h-2kb restricted, whereas line 12 had both kb and db components. both lines, and all the clones except one, could lyse targets infected with either strain a or strain b rsv. line 7 or 7-11e1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared rsv from the lungs of infected mice. there was no morbidity o ... | 1991 | 2072460 |
| nosocomial pneumonia in a canadian tertiary care center: a prospective surveillance study. | to determine the contribution of etiologic agents, including legionella pneumophila and respiratory viruses to nosocomial pneumonia at a tertiary care center. | 1991 | 2071878 |
| antiviral activities of 5-ethynyl-1-beta-d-ribofuranosylimidazole-4- carboxamide and related compounds. | a series of novel compounds, 5-alkynyl-1-beta-d-ribofuranosylimidazole-4- carboxamides, have been identified as broad-spectrum antiviral agents. 5-ethynyl-1-beta-d-ribofuranosylimidazole-4- carboxamide (eicar), the most potent congener of the group, showed antiviral potency about 10- to 100-fold greater than that of ribavirin. similar in spectrum to ribavirin, eicar was particularly active (50% inhibitory concentration, 0.2 to 4 micrograms/ml) against poxviruses (vaccinia virus), togaviruses (si ... | 1991 | 2069373 |
| early use of indirect immunofluorescence for the detection of respiratory syncytial virus in hep-2 cell culture. | respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. to detect rsv before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 hep-2 cell cultures on days 2-4 after inoculation. indirect immunofluorescent staining detected rsv in 57 of 94 cultures that eventually were found positive by cytopathic effect. in an additional 19 cases that ultimately showed no cytopathic effect, rsv also was detected. in 15 of the latter cases, the ... | 1991 | 2069130 |
| prevalence of respiratory syncytial virus subgroups a and b in france from 1982 to 1990. | a fluorescence antibody test with monoclonal antibodies was used to determine the subgroup (a or b) of respiratory syncytial virus from infants hospitalized in caen, france, over eight consecutive epidemics from 1982 to 1990. from 1982 to 1985, 27 (30%) out of 90 frozen nasal slides were classified as subgroup a strains and 63 (70%) were classified as subgroup b. b strains predominated over a in 1983-1984 and 1984-1985. from 1985 to 1990, 284 respiratory syncytial virus field strains were reisol ... | 1991 | 2064681 |
| evaluation of an enzyme membrane immunoassay (directigen rsv) for the rapid diagnosis of respiratory syncytial virus infections. | a rapid enzyme immunoassay (eia) membrane test, the directigen respiratory syncytial virus (becton dickinson), was compared with cell culture, an indirect immunofluorescence (if) test, the monofluokit respiratory syncytial virus (diagnostics pasteur), and a conventional enzyme immunoassay antigen test, the abbott respiratory syncytial virus enzyme immunoassay in nasal aspirates specimens from children with suspected respiratory syncytial virus (rsv) bronchiolitis. the sensibility and specificity ... | 1991 | 2062553 |
| group-specific serum antibody responses in children with primary and recurrent respiratory syncytial virus infections. | antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (rsv) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. plaque-reduction neutralizing (prn) antibody assays and elisas for the fusion (f) and attachment (g) glycoproteins were done using antigens of prototype rsv strains from groups a and b. responses to antigens of viruses homologous and heterologous to the antigenic group of ... | 1991 | 2056202 |
| rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: reliability as a guide to patient cohorting. | this study compared the results of a commercially available, direct fluorescent antibody (dfa) test with viral culture in 880 specimens obtained from 690 patients by means of nasopharyngeal swabs. the two tests were congruent in 92.5% (814) of the specimens. the sensitivity of the dfa was 0.95, the specificity was 0.91, the positive predictive value was 0.82, and the negative predictive value was 0.98. among 548 inpatients, there were 3 mixed infections (rsv and another virus), 8 rsv infections ... | 1991 | 2053715 |
| sequence analysis of the polymerase l gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. | the complete nucleotide sequence of the large (l) polymerase gene of human respiratory syncytial virus (rsv) strain a2 was determined by analysis of cloned-cdnas representing the entire gene and confirmed in part by dideoxy sequencing of genomic rna. the rsv l gene is 6578 nucleotides in length and contains a single major open reading frame that encodes a protein of 2165 amino acids. the molecular weight (250,226) and amino acid composition of the deduced rsv l protein are similar to those of ot ... | 1991 | 2053282 |
| nutritional and dietary interrelationships with diseases of feedlot cattle. | feedlot economics dictate that highly fermentable rations be fed, leaving the ruminant constantly on the edge of lactic acidosis. consequently, a number of subtle changes in feed, environment, or management can tip the balance, causing ruminal acidosis. even though the animal may not die from the immediate effects of a metabolic acidosis, there are sequelae such as mycotic rumenitis, hepatic abscesses, cauda vena cava thrombosis, and pulmonary arterial thromboemolism that may cause subsequent lo ... | 1991 | 2049665 |
| respiratory syncytial virus--from chimps with colds to conundrums and cures. | 1991 | 2046710 | |
| location of phosphorylated residues in human respiratory syncytial virus phosphoprotein. | the phosphoprotein (p protein) from human respiratory syncytial virus long strain, labelled in vivo with [32p]orthophosphate, was purified from virions or virus-infected human epithelial (hep-2) cells. the main phosphorylated amino acid found was serine. the determination of the n-terminal sequence of unphosphorylated and phosphorylated fragments of p protein obtained after chemical or enzymic treatments suggested that some or all of the six serines present at positions 116, 117, 119, 143, 156 a ... | 1991 | 2045795 |
| the nucleotide sequences of intergenic regions between nine genes of pneumonia virus of mice establish the physical order of these genes in the viral genome. | we have cloned eight intergenic regions from the pneumovirus pneumonia virus of mice that link the nine small and medium sized genes previously described (chambers et al., 1990). the nucleotide sequences of the clones confirm the locations of these genes and their mrna transcripts in the viral genome. the intergenic regions vary in size from 2-56 nucleotides and show only faint homology to each other or to their analogues in respiratory syncytial virus. sequence alignments suggest that the locat ... | 1991 | 2042399 |
| effect of passive antibody on the immune response of cotton rats to purified f and g glycoproteins of respiratory syncytial virus (rsv). | the effect of passively transferred rsv immune serum on the antibody response to a single dose of purified rsv fusion (f) and large (g) glycoproteins was studied in cotton rats. passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eightfold suppression of the neutralizing antibody response of cotton rats to low doses of purified f and g glycoproteins (0.2-1.7 micrograms) and a twofold suppression to higher doses ... | 1991 | 2042390 |
| [respiratory tract infection and acute deterioration of obstructive lung disease]. | respiratory infection may aggravate chronic obstructive pulmonary disease. viral respiratory infections may aggravate asthma, particularly in young individuals. respiratory syncytial virus and rhinovirus dominate in children while, in adults, influenza or rhinovirus infections are most frequently concerned. viral respiratory infections may also cause exacerbation of chronic bronchitis. bacteria and their products scarcely play any part in asthmatic disease but may possibly aggravate chronic bron ... | 1991 | 2042239 |
| comparison of two rapid methods for detection of respiratory syncytial virus (rsv) (testpack rsv and ortho rsv elisa) with direct immunofluorescence and virus isolation for the diagnosis of pediatric rsv infection. | the ability of two commercial immunoassays to detect respiratory syncytial virus (rsv) in respiratory specimens was evaluated as follows: 152 specimens were tested by testpack rsv (abbott), and 72 were tested by ortho rsv elisa (ortho). test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (dfa) alone, or virus isolation and/or dfa. testpack rsv versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (ppv), and 95% ... | 1991 | 2037684 |
| antigenic characterization of respiratory syncytial virus group a and b isolates in rio de janeiro, brazil. | the antigenic characteristics of 87 strains of respiratory syncytial virus isolated in rio de janeiro, brazil, from 1982 to 1988 were determined with a panel of monoclonal antibodies (mabs) in an enzyme immunoassay. four of these mabs immunoprecipitated the fusion protein, and five immunoprecipitated the large glycoprotein. on the basis of the patterns of reaction of these mabs to respiratory syncytial virus isolates in an enzyme immunoassay, we were able to separate isolates into the two major ... | 1991 | 2037672 |
| comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions. | nasopharyngeal secretions obtained from 94 children with acute respiratory illness were examined for the presence of respiratory syncytial virus (rsv), adenovirus, and influenza virus type a by virus culturing (virus isolation technique [vit]), immunofluorescence assay (ifa), and enzyme-linked immunosorbent assay (elisa). similar results were obtained in at least two tests for rsv, influenza virus type a, and adenovirus in 92 (97.9%), 88 (93.6%), and 88 (93.6%) cases, respectively. both rapid vi ... | 1991 | 2037663 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. | a shell vial technique was used to recover respiratory syncytial virus (rsv) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. with specimens determined to be positive by direct fluorescence assay (dfa), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). the majority of rsv isolates were detected within 16 h postinoculation, versus an average of 4.5 days by conventional techniques. also, the shell vial method recov ... | 1991 | 2037662 |
| risk factors for respiratory syncytial virus-associated lower respiratory illnesses in the first year of life. | the relation of breast feeding and other factors to the incidence of respiratory syncytial virus-associated lower respiratory tract illness (rsv-lri) in the first year of life is examined. the study population is 1,179 healthy infants enrolled at birth between may 1980 and january 1984 into the tucson children's respiratory study, tucson, arizona. each subject's data were assessed at each month of age during the first year of life, during those months when respiratory syncytial virus was isolate ... | 1991 | 2035517 |
| use of synthetic peptides to locate neutralizing antigenic domains on the fusion protein of respiratory syncytial virus. | chemical and enzymic cleavages of the f1 subunit of the fusion (f) protein of respiratory syncytial (rs) virus showed that the sequence 184-gly to 314-trp reacted with neutralizing monoclonal antibodies (mabs). twelve synthetic peptides covering a part of this sequence were analysed for their immunoreactivity with neutralizing mabs and anti-rs virus rabbit serum. two sequential antigenic domains corresponding to amino acids 200 to 225 and 255 to 278 were defined with anti-rs virus rabbit serum. ... | 1991 | 2033389 |
| development of pneumonia in desert bighorn sheep after exposure to a flock of exotic wild and domestic sheep. | from 1986 to 1989, 5 desert bighorn sheep (3 ovis canadensis mexicana and 2 o c nelsoni), ranging in age from 2 to 3 years, were exposed to a flock of exotic wild and domestic sheep to potentially achieve naturally acquired pneumonia. pasteurella multocida was isolated from nasal samples from 4 of 6 sheep randomly sampled from the flock. bighorn sheep were exposed individually and each exposure period was a trial. treatment before and after exposure varied and included combinations of alpha inte ... | 1991 | 2032914 |
| extracorporeal membrane oxygenation for nonneonatal respiratory failure. | extracorporeal membrane oxygenation (ecmo) has been used for 20 years in neonates and children with cardiac and respiratory failure. the number of neonates treated with ecmo has increased exponentially, but the number of older children treated is small. the selection and exclusion criteria for pediatric ecmo are poorly defined, and the results vary because of variable selection criteria and institutional experience with the technique. in order to help define the role of pediatric ecmo, we review ... | 1991 | 2030480 |
| cytotoxic t cell activity against the 22-kda protein of human respiratory syncytial virus (rsv) is associated with a significant reduction in pulmonary rsv replication. | recombinant vaccinia viruses expressing the rsv f glycoprotein (vac-f), or a previously described chimeric protein consisting of the extracellular domains of the f and g glycoproteins (vac-fg), or the 22-kda membrane protein (vac-22 kda) were evaluated for their ability to protect balb/c mice against infection by rsv subgroup a or subgroup b viruses and for their ability to induce a humoral immune response or a cytolytic t lymphocyte (ctl) response. immunization with vac-f or vac-fg fully protec ... | 1991 | 2024493 |
| culture vs direct antigen assays for detection of microbial pathogens from lower respiratory tract specimens suspected of containing the respiratory syncytial virus. | following the introduction of effective antiviral chemotherapy, rapid antigen assays have been utilized increasingly, instead of cell cultures, for detection of the respiratory syncytial virus from lower respiratory tract specimens. because antigen assays, unlike cell culture, cannot amplify low levels of the virus to a detectable level, assay sensitivity is especially dependent on high-quality specimens. in addition, the assays are unable to detect other viruses or bacteria with which the patie ... | 1991 | 2021312 |
| human and bovine respiratory syncytial virus: immunopathologic mechanisms. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognised as an important cause of respiratory tract disease in calves. both of these viruses and their respective diseases share many similarities. immunopathologic mechanisms have been proposed to be involved in the pathogenesis of respiratory syncytial virus (rsv) infections. this review examines the current understanding of the role of ... | 1991 | 2021054 |
| management of the infant with respiratory syncytial virus. | this article examines updated clinical information concerning respiratory syncytial virus (rsv) infection including epidemiology, pathology, clinical manifestations, diagnosis, treatment, nosocomial infection, and prognosis. also presented is current information on ribavirin therapy, its side effects, and precautions. research related to the most effective isolation methodology is discussed, as well as nursing diagnoses based on gordon's functional health patterns and interventions for the infan ... | 1991 | 2019971 |
| breast-feeding and health in the 1980s: a global epidemiologic review. | in view of the significant and articulate minority view among pediatricians that breast feeding is not "worth the bother" in developed countries, this review of the literature delves into the evidence from both developed and developing countries for the advantages of breastfeeding, both in infants and for long-term health. infants in developed settings experience twice the hospitalization rate and more severe illness from lower respiratory tract infection, primarily respiratory syncytial virus. ... | 1991 | 2019919 |
| clinical and epidemiologic aspects of respiratory syncytial virus antigenic variants in argentinian children. | 1991 | 2019767 | |
| respiratory syncytial virus infections on an adult medical ward. | eleven cases of respiratory syncytial virus (rsv) infection occurred in acutely ill hospitalized adults over a 7-week period. nosocomial illness was suspected in two patients. because rsv can cause serious infections in immunocompromised adults with the potential for nosocomial spread, the following recommendations are indicated: (1) during the winter months, early recognition and diagnosis of rsv infections both in hospital staff and in patients should be encouraged; (2) infected hospital perso ... | 1991 | 2012452 |
| antigenic and genomic diversity within group a respiratory syncytial virus. | antigenic analysis using monoclonal antibodies and genomic analysis using ribonuclease protection was done on 47 isolates of group a respiratory syncytial virus (rsv) recovered from children in st. louis during four rsv seasons. antigenic analysis identified four subgroups; of the three that included more than one member, those designated a/2 and a/2v had characteristic ribonuclease protection patterns. a third subgroup, a/4, exhibited more extensive genomic heterogeneity, but all isolates were ... | 1991 | 2010638 |
| immunity to and frequency of reinfection with respiratory syncytial virus. | to better understand the duration of immunity against respiratory syncytial virus (rsv) and the role of serum antibodies to the surface glycoproteins, f and g, in susceptibility to reinfection, 15 adults with previous natural rsv infection were challenged with rsv of the same strain group (a) at 2, 4, 8, 14, 20, and 26 months after natural infection. by 2 months about one-half and by 8 months two-thirds of the subjects became reinfected. each challenge resulted in infection in at least one-fourt ... | 1991 | 2010624 |
| multicenter study of strains of respiratory syncytial virus. | two major groups of respiratory syncytial virus (rsv) strains, a and b, have been identified and their patterns of isolation determined in different communities but not simultaneously in multiple communities. in this study, we tested 483 rsv isolates from 14 university laboratories in the united states and canada for the 1984/1985 and 1985/1986 rsv seasons; 303 (63%) isolates were group a, 114 (24%) were group b, and 66 (14%) could not be grouped. isolates were subdivided into six subgroups with ... | 1991 | 2010623 |
| dual-enzyme cascade-magnetic separation immunoassay for respiratory syncytial virus. | a new immunoassay developed for the detection of respiratory syncytial virus (rsv) makes use of magnetic separation and amplification by a dual-enzyme cascade for signal generation. magnetic particles are conjugated to monoclonal anti-rsv antibodies through the heterobifunctional crosslinker sulfosuccinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, yielding particles of high specific activity and low background. the dual-enzyme cascade is initiated by activation of a masked inhibitor for t ... | 1991 | 2010616 |
| evaluation of immunofluorescence, two enzyme immunoassays and the shell-vial assay for detection of respiratory syncytial virus. | 1991 | 2009879 | |
| [virologic diagnosis of interstitial pneumonia]. | viral pneumonia is not very frequent in healthy adults; it is mostly observed in elderly subjects infected with an influenza virus, a respiratory syncytial virus (rsv) or an adenovirus (adv). in immunocompromised subjects, cytomegalovirus (cmv) is the most common of causal agents. from the point of view of clinicians, virological diagnostic methods must be rapid and specific, and for this reason direct examination techniques for respiratory specimens and immunological detection of virus are curr ... | 1991 | 2008581 |
| characterization of oligosaccharide structures on a chimeric respiratory syncytial virus protein expressed in insect cell line sf9. | the oligosaccharide structures added to a chimeric protein (fg) composed of the extracellular domains of respiratory syncytial virus f and g proteins, expressed in the insect cell line sf9, were investigated. cells were labeled in vivo with [3h]glucosamine and infected with a recombinant baculovirus containing the fg gene. the secreted chimeric protein was isolated by immunoprecipitation and subjected to oligosaccharide analysis. the fg protein contains two types of o-linked oligosaccharides: ga ... | 1991 | 2007124 |
| respiratory syncytial virus infection in infants with unequal pulmonary perfusion. | 1991 | 2005950 | |
| the g protein of human respiratory syncytial virus: significance of carbohydrate side-chains and the c-terminal end to its antigenicity. | the reactivities of eighteen monoclonal antibodies with different glycosylated forms of the human respiratory syncytial (rs) virus g protein were tested in western blots. only five antibodies recognized the unglycosylated precursor. the majority of antibodies, however, reacted with the o-glycosylated form of the g protein, emphasizing the importance of this type of modification for the antigenicity of the mature molecule. human antisera, which recognized the rs virus g protein in western blots, ... | 1991 | 2005433 |
| respiratory syncytial virus infection of human mononuclear phagocytes stimulates synthesis of platelet-activating factor. | production of platelet-activating factor 1-o-alkyl-2-acetyl-sn-glycero-3- phosphocholine (paf), a potent mediator of inflammation, by mononuclear phagocytes varies with their stage of cellular differentiation and the nature of the eliciting stimulus. the human monocytic cell line u937 can be induced to differentiate to a macrophage-like cell following phorbol myristate acetate exposure, and after differentiation, these cells efficiently support replication of respiratory syncytial virus (rsv). u ... | 1991 | 2005091 |
| etiology of acute lower respiratory tract infections in gambian children: ii. acute lower respiratory tract infection in children ages one to nine years presenting at the hospital. | seventy-four children ages 1 to 9 years hospitalized because of severe pneumonia were investigated using blood cultures, lung aspirates, nasopharyngeal aspirates, serology and antigen detection procedures. a bacterial infection was identified in 57 (77%), a viral infection was seen in 25 (34%) and 18 (24%) had mixed viral-bacterial infections. the bacterial pathogens most frequently identified were streptococcus pneumoniae and haemophilus influenzae found in 61 and 15% of patients, respectively. ... | 1991 | 2003054 |
| handwashing and cohorting in prevention of hospital acquired infections with respiratory syncytial virus. | hospital acquired infections with respiratory syncytial virus are a major problem. the virus is spread predominantly by infected nasal secretions and we investigated whether we could reduce its incidence by cohorting babies on each ward into designated areas and encouraging staff and parents to wash their hands. we examined the incidence of hospital acquired infection due to respiratory syncytial virus in all children less than 2 years old and in those with congenital heart disease. in 1986-7, b ... | 1991 | 2001109 |
| respiratory syncytial virus infections at rhode island hospital: use of ribavirin. | 1991 | 2000472 | |
| respiratory syncytial virus (rsv) f, g, m2 (22k), and n proteins each induce resistance to rsv challenge, but resistance induced by m2 and n proteins is relatively short-lived. | the ability of recombinant vaccinia viruses that separately encoded 9 of the 10 known respiratory syncytial virus (rsv) proteins to induce resistance to rsv challenge was studied in balb/c mice. resistance was examined at two intervals following vaccination to examine early (day 9) as well as late (day 28) immunity. balb/c mice were inoculated simultaneously by the intranasal and intraperitoneal routes with a recombinant vaccinia virus encoding one of the following rsv proteins: f, g, n, p, sh, ... | 1991 | 1995956 |
| age-related prevalence of serum antibody to respiratory syncytial virus in ecuadorian and german children. | 1991 | 1995747 | |
| vaccination of cotton rats with a chimeric fg glycoprotein of human respiratory syncytial virus induces minimal pulmonary pathology on challenge. | the cotton rat model of human respiratory syncytial virus (rsv) infection was used to study the safety and efficacy of a chimeric fg glycoprotein that was expressed in insect cells using a baculovirus vector. histologic and virologic examination of vaccinated rat lungs was done after challenge with rsv. when rats were challenged 1 month after vaccination, severe pulmonary inflammation characterized by both a mononuclear and polymorphonuclear cell infiltrate and 30%-40% involvement of lung tissue ... | 1991 | 1995720 |
| pattern of respiratory syncytial virus epidemics in finland: two-year cycles with alternating prevalence of groups a and b. | time-resolved fluoroimmunoassay with monoclonal antibodies distinguishing between respiratory syncytial virus (rsv) group a and b strains was used to analyze their prevalence in finland during 1981-1990 among 3285 patients with laboratory diagnosis of rsv, most of them hospitalized. the group typing of antigens in 608 rsv-positive nasopharyngeal aspirates showed a regular alternation of group prevalence, following the cyclic occurrence of the virus. group a predominated in 73%-90% of specimens f ... | 1991 | 1995719 |
| nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mrna and expression from a recombinant vaccinia virus. | bovine respiratory syncytial (brs) virus is an important cause of serious respiratory illness in calves. the disease caused in calves is similar to that caused by human respiratory syncytial (hrs) virus in children. the two viruses, however, have distinct host ranges and the attachment glycoproteins, g, have no antigenic cross-reactivity. the fusion glycoproteins, f, of the hrs and brs viruses, however, have some antigenic cross-reactivity. to further compare the brs virus and hrs virus fusion p ... | 1991 | 1994571 |
| antiviral effects of xanthate d609 on the human respiratory syncytial virus growth cycle. | the antiviral compound tricyclo-decan-9-yl-xanthogenate (d609) inhibits respiratory syncytial (rs) virus growth in human epithelial (hep 2) cells. d609 treatment resulted in a decrease in the accumulation of viral proteins, in the phosphorylation of the viral phosphoprotein, and in the amount of extracellular antigens and infectious particles. the relative accumulation of viral proteins was also unbalanced, however no differences were found in the amount of viral rna with plus or minus polarity. ... | 1991 | 1994569 |
| respiratory syncytial virus heterogeneity during an epidemic: analysis by limited nucleotide sequencing (sh gene) and restriction mapping (n gene). | the genes encoding the small hydrophobic (sh) proteins of a series of respiratory syncytial (rs) virus strains were amplified using the polymerase chain reaction, cloned and sequenced. analysis of the sh gene sequences from 12 rs virus strains isolated between 1956 and 1989 confirmed the homogeneity of the two subgroups. a and b, previously defined serologically. although there is only 76% deduced amino acid sequence identity of sh proteins between subgroups, there was little variation in deduce ... | 1991 | 1993875 |
| a single-season epidemic with respiratory syncytial virus subgroup b2 during 10 epidemic years, 1978 to 1988. | respiratory syncytial virus strains isolated from infants and children hospitalized with acute respiratory tract disease in huntington, w. va., from july 1979 through june 1988 were categorized by subgroup by using two panels of monoclonal antibodies specific for subgroup a and subgroup b. of 405 strains tested, 319 (78.8%) were subgroup a, 73 (18.0%) were subgroup b1, and 13 (3.2%) were subgroup b2. the subgroup b2 strains occurred as a single-season epidemic during the epidemiologic year july ... | 1991 | 1993752 |
| nosocomial transmission of respiratory syncytial virus in immunocompromised adults. | respiratory syncytial virus (rsv) isolates obtained from nine infected immunocompromised adult patients hospitalized during two consecutive winters (january through april 1987 and 1988) were collected and analyzed against a panel of monoclonal antibodies by an enzyme immunoassay. the history of the patients' illness, onset of symptoms, and date of initial isolation of virus was correlated with the hospital ward and time of hospitalization. three patients died of respiratory failure related to rs ... | 1991 | 1993745 |
| deduced amino acid sequence of the fusion glycoprotein of turkey rhinotracheitis virus has greater identity with that of human respiratory syncytial virus, a pneumovirus, than that of paramyxoviruses and morbilliviruses. | the sequence of the fusion (f) glycoprotein of turkey rhinotracheitis virus (trtv) has been deduced from cdna clones derived from oligo(dt)-selected infected cell rna. the protein consists of 538 amino acids, the f2 and f1 subunits containing 102 (including the f2-f1 connecting peptide rrrr) and 436 residues, respectively. each subunit has one potential n-linked glycosylation site. the protein has 38 to 39% amino acid identity with the f protein of respiratory syncytial virus (pneumovirus genus) ... | 1991 | 1990068 |