Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| quantitative pcr for listeria monocytogenes with colorimetric detection. | an enzyme-linked immunosorbent assay (elisa)-mediated polymerase chain reaction (pcr) technique was developed to detect and quantify listeria monocytogenes in food products. the bacterial dna was extracted from artificially contaminated food and co-amplified with a synthetic internal standard (is) using primers specific for the target gene coding for the invasive-associated protein (i.a.p.), a virulence factor of l. monocytogenes (i.a.p.) or is in the presence of fluorescein-dutp pcr products we ... | 1999 | 9921826 |
| update: multistate outbreak of listeriosis--united states, 1998-1999. | from early august 1998 through january 6, 1999, at least 50 illnesses caused by a rare strain of the bacterium listeria monocytogenes, serotype 4b, have been reported to cdc by 11 states. six adults have died and two pregnant women have had spontaneous abortions. reported illness onset dates were during august 2-december 13, 1998. cdc and state and local health departments have identified the vehicle for transmission as hot dogs and possibly deli meats produced under many brand names by one manu ... | 1999 | 9921730 |
| restraint stress-induced immunosuppression by inhibiting leukocyte migration and th1 cytokine expression during the intraperitoneal infection of listeria monocytogenes. | in this study, a murine model of listeria monocytogenes infection was used to investigate effects of restraint stress (rst) on host defense. we observed that the l. monocytogenes infection as well as rst induced an elevation of endogenous corticosterone (cort) levels and rst synergistically enhanced endogenous cort levels during the listerial infection. rst suppressed the migration of leukocytes including macrophages, neutrophils, nk cells and lymphocytes into the peritoneal cavities after the i ... | 1998 | 9916889 |
| perforin-deficient cd8+ t cells: in vivo priming and antigen-specific immunity against listeria monocytogenes. | cd8+ t cells require perforin to mediate immunity against some, but not all, intracellular pathogens. previous studies with h-2b mhc perforin gene knockout (po) mice revealed both perforin-dependent and perforin-independent pathways of cd8+ t cell-mediated immunity to listeria monocytogenes (lm). in this study, we address two previously unresolved issues regarding the requirement for perforin in antilisterial immunity: 1) is cd8+ t cell-mediated, perforin-independent immunity specific for a sing ... | 1999 | 9916723 |
| il-12 is not required for induction of type 1 cytokine responses in viral infections. | to investigate the physiological role of il-12 in viral infections in terms of t cell cytokine responses involved in virus-specific ig isotype induction and in antiviral protection, immune responses elicited upon infection of il-12-deficient mice with lymphocytic choriomeningitis virus (lcmv) or vesicular stomatitis virus (vsv) were studied. infection of il-12-deficient mice with lcmv induced a virus-specific type 1 cytokine response as determined by in vitro cytokine secretion patterns as well ... | 1999 | 9916721 |
| experimental mixed infection with yersinia enterocolitica and listeria monocytogenes in guinea pigs. | the pathogenesis and the cell immune response (cir) of guinea pigs after mixed infection with y. enterocolitica and l. monocytogenes was investigated. the guinea pigs were infected per os with 1.1 x 10(9) cfu y. enterocolitica 0:3, (pyv+) and four days later with 1.1 x 10(9) cfu l. monocytogenes 4b. clinical, paraclinical and morphological findings attending the infectious process were followed in dynamics up to the 28th day post infection (p.i.) with l. monocytogenes. the phagocyting activity o ... | 1998 | 9916552 |
| induction of protective t cells against listeria monocytogenes in mice by immunization with a listeriolysin o-negative avirulent strain of bacteria and liposome-encapsulated listeriolysin o. | only listeriolysin o (llo)-producing strains of listeria monocytogenes generate protective immunity in mice. based on the findings that endogenous gamma interferon (ifn-gamma) production was induced only by such strains and that purified llo could induce ifn-gamma from nk cells, we have postulated that llo may play a pivotal role in the induction of th1-type protective t cells, which are highly dependent on ifn-gamma. in this study, mice were immunized with l. monocytogenes atcc 15313, an llo-no ... | 1999 | 9916060 |
| cytotoxic t lymphocytes in resistance to tuberculosis. | recent experimental evidence has suggested t cells recognizing antigens in the context of both classical mhc class i and nonclassical class i-like molecules contribute to protective responses against mycobacterium tuberculosis (mtb) infection. our aims were to characterize both types of t cells, and to explore the basis of communication between the tubercle bacilli and the mhc class i pathway of the host macrophage. a model system was developed using exogenously added ovalbumin as a surrogate an ... | 1998 | 9889963 |
| [the microbiological quality of purified sludge intended for agriculture: its conformity to clause iib of d.l. 99/92]. | 1998 | 9887542 | |
| [study on bacteremia due to community-acquired infection in infants and children without underlying diseases]. | between july 1988 and june 1998, 22 pediatric patients without underlying diseases were diagnosed as bacteremia caused by community-acquired infection in asahikawa kosei hospital. the age range of the patients was from 7 days to 4 years. their diseases were meningitis in 6, urinary tract infection in 6, respiratory tract infection in 4, skin infection in 2, and unknown origin in 4. the causative organisms were escherichia coli in 7, haemophilus influenzae in 4, streptococcus pneumoniae in 4, sta ... | 1998 | 9884506 |
| the transcriptional regulator gene phrr in sinorhizobium meliloti wsm419 is regulated by low ph and other stresses. | the phrr gene in sinorhizobium meliloti (previously known as rhizobium meliloti) wsm419, directly downstream from acta, is induced by low ph or certain stresses (e.g. high concentrations of zn2+, cu2+, h2o2 or ethanol), but not in stationary phase or by other stresses (e.g. phosphate limitation, elevated temperature, high concentrations of sucrose or iron). a dna fragment containing the wild-type phrr gene could not be cloned and inverse pcr was therefore used to amplify a 3.5 kb bamhi fragment ... | 1998 | 9884225 |
| multistate outbreak of listeriosis--united states, 1998. | since early august 1998, 40 illnesses caused by a single strain of listeria monocytogenes (lm) have been identified in 10 states: ohio (13 cases); new york; tennessee, massachusetts, and west virginia (three each); michigan (two); and connecticut, oregon, vermont, and georgia (one each). dates of illness onset or lm isolation ranged from august 2 through december 2. all lm isolates from these cases are serotype 4b and share an unusual pattern when subtyped either by pulsed-field gel electrophore ... | 1998 | 9883769 |
| cell wall teichoic acid glycosylation in listeria monocytogenes serotype 4b requires gtca, a novel, serogroup-specific gene. | we have identified a novel gene, gtca, involved in the decoration of cell wall teichoic acid of listeria monocytogenes serotype 4b with galactose and glucose. insertional inactivation of gtca brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. interestingly, the composition of membrane-associated lipoteichoic acid was not affected. complemen ... | 1999 | 9882654 |
| intramedullary brain stem cyst and trapped iv ventricle after infection with listeria monocytogenes. | we present the rare case of a girl surviving intrauterine listeria brain stem meningoencephalitis, who subsequently developed hydrocephalus, a trapped iv ventricle and an intramedullary cyst. such cases have been reported only infrequently, and in earlier cases modern imaging studies were not available. magnetic resonance imaging has been helpful in our patient to delineate the lesions and plan further treatment. | 1998 | 9881629 |
| quantitative risk assessment of human listeriosis from consumption of soft cheese made from raw milk. | microbial hazards have been identified in soft cheese made from raw milk. quantification of the resulting risk for public health was attempted within the frame of the codex alimentarius commission, 1995 approach to quantitative risk assessment, using monte carlo simulation software. quantitative data could only be found for listeria monocytogenes. the complete process of cheese making was modeled, from milking to consumption. using data published on the different sources of milk contamination (e ... | 1998 | 9879587 |
| detection of listeria monocytogenes in humans, animals and foods. | the listeria monocytogenes-carrying rates were 100% for listeriosis patients and 1.3% for healthy humans. the l. monocytogenes contamination rates for retail sliced beef (34.2%) and pork (36.4%) were significantly higher (p < 0.05) than those for cattle (2.0%) and pigs (0.8%) and for cattle (4.9%) and swine (7.4%) carcasses. the percentages of serotypes 1/2a, 1/2b and 4b which are most dominant in human patients were high in isolates from fresh (90.0%) and processed (100%) fish and shellfish and ... | 1998 | 9879536 |
| nitric oxide mediates immunosuppression induced by listeria monocytogenes infection: quantitative studies. | our laboratory has shown that immunization of mice with an attenuated strain of salmonella typhimuriuminduces profound suppression in the capacity of splenocytes to mount an in vitro antibody plaque-forming cell (pfc) response to sheep red blood cells (srbc) and to proliferate in response to mitogens. in vitro addition of ng-monomethyl-l-arginine (nmma), an inhibitor of nitric oxide (no) synthase, to cell cultures from salmonella-immunized mice completely blocked suppression of the pfc responses ... | 1998 | 9878455 |
| 2-deoxy-d-glucose-induced metabolic stress enhances resistance to listeria monocytogenes infection in mice. | exposure to different forms of psychological and physiological stress can elicit a host stress response, which alters normal parameters of neuroendocrine homeostasis. the present study evaluated the influence of the metabolic stressor 2-deoxy-d-glucose (2-dg; a glucose analog, which when administered to rodents, induces acute periods of metabolic stress) on the capacity of mice to resist infection with the facultative intracellular bacterial pathogen listeria monocytogenes. female bdf1 mice were ... | 1998 | 9877421 |
| shelf life extension, safety, and quality of fresh pork loin treated with high hydrostatic pressure. | the optimal conditions of pressure, time, and processing temperature required to eliminate listeria monocytogenes scott a and salmonella typhimurium atcc 13311 in fresh pork loin and the effect of these optimal conditions on quality and shelf life were determined. twenty-five grams of fresh pork loin were inoculated with either of the two organisms and were subjected to pressures between 414 and 827 mpa at either 2 or 25 degrees c for 30 min. the d414mpa(25 degrees c) was determined to be 2.17 m ... | 1998 | 9874343 |
| growth of listeria monocytogenes and yersinia enterocolitica on cooked modified-atmosphere-packaged poultry in the presence and absence of a naturally occurring microbiota. | cooked poultry cuts were inoculated with five-strain composite mixtures of either listeria monocytogenes or yersinia enterocolitica (1,000 cfu/150-g piece), packaged in 44:56 co2-n2, and stored at 3.5, 6.5, or 10 degrees c for up to 5 weeks. both l. monocytogenes and y. enterocolitica grew under all test conditions. the presence of a naturally occurring microbiota did not influence the growth of either pathogen. addition of lactate with the shelf life extender alta 2341 lengthened the lag phases ... | 1999 | 9872806 |
| bacterial resistance to ultrasonic waves under pressure at nonlethal (manosonication) and lethal (manothermosonication) temperatures. | the decimal reduction times of streptococcus faecium, listeria monocytogenes, salmonella enteritidis, and aeromonas hydrophila corresponding to heat treatment at 62 degrees c were 7.1, 0.34, 0.024, and 0.0096 min, and those corresponding to manosonication treatment (40 degrees c, 200 kpa, 117 microm) were 4.0, 1.5, 0.86, and 0.90 min, respectively. the manosonication decimal reduction times of the four species investigated decreased sixfold when the amplitude was increased from 62 to 150 microm ... | 1999 | 9872795 |
| sources of listeria monocytogenes contamination in a cold-smoked rainbow trout processing plant detected by pulsed-field gel electrophoresis typing. | sites of listeria monocytogenes contamination in a cold-smoked rainbow trout (oncorhynchus mykiss) processing plant were detected by sampling the production line, environment, and fish at different production stages. two lots were monitored. the frequency of raw fish samples containing l. monocytogenes was low. during processing, the frequency of fish contaminated with l. monocytogenes clearly rose after brining, and the most contaminated sites of the processing plant were the brining and postbr ... | 1999 | 9872773 |
| reduction of caecal listeria monocytogenes in leghorn chicks following treatment with a competitive exclusion culture (preempt) | 1998 | 9871361 | |
| natural milk fatty acids affect survival and invasiveness of listeria monocytogenes. | the effects of 12 fatty acids, naturally occurring in milk from several mammalian species, on the survival and invasion ability of listeria monocytogenes, a food-borne pathogen, were determined. the survival was tested in the presence of 200 micrograms ml-1 fatty acids suspended in brain hearth infusion broth or in storage conditioning solution (nacl 1%) of mozzarella cheese, an italian soft unripened cheese, at ph 7.0 or 5.0. lauric (c12:0), linoleic (c18:2) and linolenic (c18:3) acids exerted ... | 1998 | 9871355 |
| synergist effect of sucrose fatty acid esters on nisin inhibition of gram-positive bacteria. | nisin in combination with the sucrose fatty acid esters, sucrose palmitate (p-1570 and p-1670) or sucrose stearate (s-1570 and s-1670) was tested against a range of gram-negative and gram-positive bacteria. initial liquid culture investigation showed that the sugar ester p-1670 resulted in a synergist enhancement of the bacteriostatic activity of nisin against gram-positive bacteria and not gram-negative bacteria. some enhancement of the bactericidal activity of nisin against listeria monocytoge ... | 1998 | 9871322 |
| genetic analysis of the bacteriocin-encoding plasmids prj6 and prj9 of staphylococcus aureus by transposon mutagenesis and cloning of genes involved in bacteriocin production. | prj6 and prj9, small staphylococcus aureus plasmids which code for bacteriocins, exhibited a bactericidal activity against several lactic acid bacteria and strains of listeria monocytogenes, an important food-borne pathogen. filter-mating experiments using plasmid derivatives tagged with either tn551 or tn917-lac showed that prj6, but not prj9, could be mobilized by staphylococcal conjugative plasmids. transposon mutagenesis of both plasmids was also performed. the bacteriocin and immunity struc ... | 1998 | 9871317 |
| characterization of a large motility gene cluster containing the cher, motab genes of listeria monocytogenes and evidence that prfa downregulates motility genes. | through the analysis of a non-motile mutant of listeria monocytogenes, we identified and characterized a locus containing the cher, mota and motb genes. these three genes are homologous to the cher, and mota/b genes of bacillus subtilis which in this organism are 954 kb apart. the gene organization in listeria is also not similar either to that of escherichia coli in which cher and motab are 5.9 kb apart. cher and mota/b, as previously reported for flaa, the flagellin gene, are thermoregulated w ... | 1998 | 9868779 |
| [mechanisms of induction and expression of anti-tuberculous immunity]. | the induction of anti-tuberculous immunity highly depends on the cytokines produced endogenously at the initial stage of immunization. among several cytokines, ifn-gamma appears to be the most important to generate antigen-specific th1 type of protective t cells in mice. il-12 and il-18, which are produced by macrophages in response to virulent mycobacteria, are responsible for stimulating nk cells to produce ifn-gamma. once antigen-specific th1 cells are generated, th1-dependent macrophage acti ... | 1998 | 9866925 |
| [a case of maternal and neonatal infection due to listeria monocytogenes]. | a listeria monocytogenes infection may develop during pregnancy by eating sausages, fresh meats and milk products derived from infected animals. according to the period in which the infection starts, the pregnancy outcome can be abortion or pre-term or at term delivery. the infection can pass from mother to fetus and can cause a serious neonatal sepsis. listeriosis in pregnant women can be asymptomatic or may present as an influenza-like syndrome. this case report, along with other published cas ... | 1998 | 9866893 |
| spatial control of actin filament assembly: lessons from listeria. | 1998 | 9865692 | |
| heme oxygenase inhibits nitric oxide synthase by degrading heme: a negative feedback regulation mechanism for nitric oxide production. | 1998 | 9865340 | |
| existing antilisterial immunity does not inhibit the development of a listeria monocytogenes-specific primary cytotoxic t-lymphocyte response. | infection of balb/c mice with listeria monocytogenes stimulates an antilisterial immune response evident by the appearance of h2-kd-restricted cd8(+) cytotoxic t lymphocytes (ctls) specific for the nanomer peptides amino acids (aa) 91 to 99 of listeriolysin o (llo 91-99) and aa 217 to 225 of the p60 molecule (p60 217-225). we have introduced point mutations at anchor residues within llo 91-99 (92f) or p60 217-225 (218f), and balb/c mice infected with l. monocytogenes strains containing these poi ... | 1999 | 9864223 |
| mutagenesis of active-site histidines of listeria monocytogenes phosphatidylinositol-specific phospholipase c: effects on enzyme activity and biological function. | listeria monocytogenes, a gram-positive facultative intracellular pathogen, produces two distinct phospholipases c. pc-plc, encoded by plcb, is a broad-range phospholipase, whereas pi-plc, encoded by plca, is specific for phosphatidylinositol. it was previously shown that pi-plc plays a role in efficient escape of l. monocytogenes from the primary phagosome. to further understand the function of pi-plc in intracellular growth, site-directed mutagenesis of plca was performed. two potential active ... | 1999 | 9864213 |
| expression of listeriolysin o and acta by intracellular and extracellular listeria monocytogenes. | listeria monocytogenes requires listeriolysin o (llo) and acta, the products of hly and acta, respectively, to establish a productive intracellular infection. llo is essential for vacuolar lysis and entry into the cytosol, while acta is required for bacterial spread to adjacent cells. we have used a transcriptional reporter gene system to compare the expression of acta and hly during intracellular growth to that during growth in broth cultures. the hly and acta genes were transcriptionally fused ... | 1999 | 9864207 |
| two families of gtpases dominate the complex cellular response to ifn-gamma. | ifn-gamma induces a number of cellular programs functional in innate and adaptive resistance to infectious pathogens. it has recently become clear that the complete cellular response to ifn-gamma is extraordinarily complex, with >500 genes (i.e., approximately 0.5% of the genome) activated. we made suppression-subtractive hybridization differential libraries from ifn-gamma-stimulated primary mouse embryonic fibroblasts and from a mouse macrophage cell line, ana-1, in each case with reference to ... | 1998 | 9862701 |
| the gene cluster inlc2de of listeria monocytogenes contains additional new internalin genes and is important for virulence in mice. | in this work we identified and characterized a gene cluster containing three internalin genes of listeria monocytogenes egd. these genes, termed inlg, inlh and inle, encode proteins of 490, 548 and 499 amino acids, respectively, which belong to the family of large, cell wall-bound internalins. the inlghe gene cluster is flanked by two listerial house-keeping genes encoding proteins homologous to the 6-phospho-beta-glucosidase and the succinyl-diaminopimelate desuccinylase of e. coli. a similar i ... | 1998 | 9862466 |
| inhibition of bacterial pathogens by lactobacilli. | lactobacilli produce many antimicrobial substances including organic acids, hydrogen peroxide and bacteriocins. antagonistic activity of lactobacilli is an important factor in the protection of the vagina of fertile women against infection by certain pathogens. in the present study, we investigated 17 strains of lactobacilli, including 11 strains of vaginal origin. the aim was to investigate in more detail the antibacterial activity of lactobacilli and to attempt to assess substances responsible ... | 1998 | 9861683 |
| typing of listeria monocytogenes strains by repetitive element sequence-based pcr. | listeria monocytogenes strains possess short repetitive extragenic palindromic (rep) elements and enterobacterial repetitive intergenic consensus (eric) sequences. we used repetitive element sequence-based pcr (rep-pcr) to evaluate the potential of rep and eric elements for typing l. monocytogenes strains isolated from humans, animals, and foods. on the basis of rep-pcr fingerprints, l. monocytogenes strains were divided into four major clusters matching origin of isolation. rep-pcr fingerprints ... | 1999 | 9854072 |
| functional similarities between the listeria monocytogenes virulence regulator prfa and cyclic amp receptor protein: the prfa* (gly145ser) mutation increases binding affinity for target dna. | most listeria monocytogenes virulence genes are positively regulated by the prfa protein, a transcription factor sharing sequence similarities with cyclic amp (camp) receptor protein (crp). its coding gene, prfa, is regulated by prfa itself via an autoregulatory loop mediated by the upstream prfa-dependent plca promoter. we have recently characterized prfa* mutants from l. monocytogenes which, as a result of a single amino acid substitution in prfa, gly145ser, constitutively overexpress prfa and ... | 1998 | 9852011 |
| irradiation as a method for decontaminating food. a review. | despite substantial efforts in avoidance of contamination, an upward trend in the number of outbreaks of foodborne illnesses caused by nonsporeforming pathogenic bacteria are reported in many countries. good hygienic practices can reduce the level of contamination but the most important pathogens cannot presently be eliminated from most farms nor is it possible to eliminate them by primary processing, particularly from those foods which are sold raw. several decontamination methods exist but the ... | 1998 | 9851599 |
| initial numbers, serovars and phagevars of listeria monocytogenes isolated in prepared foods in the city of barcelona (spain). | a total of 1100 samples of prepared foods purchased in restaurants and delicatessen shops in the city of barcelona was examined for the presence of listerio. l. monocytogenes was more frequently isolated from foods intended to be consumed without further cooking (9.3%) than from foods intended to receive further cooking prior to consumption (2.9%). a quantitative study, carried out with 773 samples, yielded 1% of samples with numbers of l. monocytogenes higher than 100 cfu/g. strains of l. monoc ... | 1998 | 9849792 |
| the effect of the growth environment on the lag phase of listeria monocytogenes. | the duration of lag in listeria monocytogenes was examined in relation to the physico-chemical properties of the growth environment. it was supposed that lag would be determined by two hypothetical quantities, the amount of work that a cell has to perform to adapt to new conditions and the rate at which it can perform that work. if the rate at which the cell can perform the necessary work is a function of the maximum specific growth rate in the new environment, the hypothesis predicts that lag t ... | 1998 | 9849786 |
| listeria monocytogenes causing solitary liver abscess. case report and review of the literature. | the authors report on a case of a solitary liver abscess due to listeria monocytogenes in a 53-year-old diabetic white male and review all published cases of solitary listerial abscesses of the liver. l. monocytogenes is a rare cause of solitary liver abscess which occurs in elderly patients with diabetes mellitus. the clinical signs are variable and often mimic malignancy, with epigastric pain, night sweats and weight loss. prevalent features are poor control of glycemia, temperature up to 38.5 ... | 1998 | 9845615 |
| the suitability and application of a gfp-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells. | the product of a gfp-actin gene fusion, permanently or transiently transfected in diverse mammalian cell lines, was shown to be a suitable, intrinsic probe of both the organization and dynamics of the actin cytoskeleton. in live swiss 3t3 and nih 3t3 cells, the fusion protein was found to accumulate in lamellipodia, filopodia, focal contacts and stress fibers. furthermore, comparisons of fluorescence images of gfp-actin and cy3.5-phalloidin, an independent marker of f-actin, in permeabilized cel ... | 1998 | 9840457 |
| effect of feline immunodeficiency virus on cytokine response to listeria monocytogenes in vivo. | feline immunodeficiency virus (fiv) is a lentivirus that induces an acquired immunodeficiency in domestic cats. the objective of this study was to compare the immune response of chronically fiv-infected cats and specific pathogen free (spf) cats to listeria monocytogenes, a facultative intracellular bacterium. regional lymph nodes were removed at various times after subcutaneous inoculation with l. monocytogenes and evaluated. lymph nodes of chronically fiv-infected cats enlarged more slowly and ... | 1998 | 9839868 |
| antimicrobial effect of enterocin ccm 4231 in the cattle slurry environment. | the antagonistic effect of enterocin ccm 4231 towards enterococci, staphylococci, escherichia coli, listeriae and pseudomonads in the cattle slurry environment was assessed during periods of 1 and 2 weeks. the maximum decrease in the viable cells of enterococci and staphylococci (5.39 to 1.1 log cfu ml-1, and 4.3 to 2.3 log cfu ml-1, respectively) was detected on the second day after enterocin ccm 4231 addition to cattle slurry. e. coli cells, listeriae and pseudomonads decreased insignificantly ... | 1998 | 9839432 |
| evaluation of universal preenrichment broth for the recovery of foodborne pathogens from milk and cheese. | the use of universal preenrichment broth for the recovery of verotoxigenic escherichia coli, salmonella spp., and listeria monocytogenes from milk and cheese was examined. universal preenrichment broth supported the growth of low inoculum levels (10 cfu/ml) of these organisms in pure cultures and in mixed cultures containing higher levels of other pathogens or bacterial flora from raw milk. this medium also supported the recovery and growth of heat-injured salmonella spp., l. monocytogenes, and ... | 1998 | 9839221 |
| purification and genetic characterization of enterocin i from enterococcus faecium 6t1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins. | enterocin i (enti) is a novel bacteriocin produced by enterococcus faecium 6t1a, a strain originally isolated from a spanish-style green olive fermentation. the bacteriocin is active against many olive spoilage and food-borne gram-positive pathogenic bacteria, including clostridia, propionibacteria, and listeria monocytogenes. enti was purified to homogeneity by ammonium sulfate precipitation, binding to an sp-sepharose fast-flow column, and phenyl-sepharose cl-4b and c2/c18 reverse-phase chroma ... | 1998 | 9835578 |
| use of a genetically enhanced, pediocin-producing starter culture, lactococcus lactis subsp. lactis mm217, to control listeria monocytogenes in cheddar cheese | cheddar cheese was prepared with lactococcus lactis subsp. lactis mm217, a starter culture which contains pmc117 coding for pediocin pa-1. about 75 liters of pasteurized milk (containing ca. 3.6% fat) was inoculated with strain mm217 (ca. 10(6) cfu per ml) and a mixture of three listeria monocytogenes strains (ca. 10(3) cfu per ml). the viability of the pathogen and the activity of pediocin in the cheese were monitored at appropriate intervals throughout the manufacturing process and during ripe ... | 1998 | 9835572 |
| in vitro activities of ketolide hmr 3647, macrolides, and clindamycin against coryneform bacteria. | the in vitro activity of ketolide hmr 3647 against coryneform bacteria isolated from clinical samples was evaluated. except against corynebacterium jeikeium and c. urealyticum, hmr 3647 showed high activity against corynebacterium spp., being more active than 14- and 16-membered macrolides, azithromycin, or clindamycin. hmr 3647 also had high in vitro activity against brevibacterium spp. and listeria monocytogenes. | 1998 | 9835530 |
| [listeria monocytogenes rhomboencephalitis. clinical case]. | we report a previously healthy 44 years old female, that presented with mild clouding of consciousness, a left cerebellar syndrome, involvement of v, x and xii left cranial nerves and an alteration of epicritic sensitivity in the left half of the body. cerebrospinal fluid had inflammatory features. cerebrospinal fluid and blood cultures were positive for listeria monocytogenes. magnetic resonance imaging disclosed a rhomboencephalitis. antibiotics were started and the clinical condition of the p ... | 1998 | 9830776 |
| variations in tolerance of listeria monocytogenes to nisin, pediocin pa-1 and bavaricin a. | a collection of 381 listeria monocytogenes strains was examined for strain variations in nisin and pediocin sensitivity at three different concentrations on tryptic soya agar. two of the strains were able to grow on agar plates containing 500 iu nisin ml-1. these strains were characterized as having an enhanced nisin tolerance. twenty strains had normal growth on agar plates containing 1600 au pediocin ml-1 and higher. these strains were characterized as pediocin-resistant. another 34 strains, c ... | 1998 | 9830144 |
| a homolog of ccpa mediates catabolite control in listeria monocytogenes but not carbon source regulation of virulence genes. | readily utilizable sugars down-regulate virulence gene expression in listeria monocytogenes, which has led to the proposal that this regulation may be an aspect of global catabolite regulation (cr). we recently demonstrated that the metabolic enzyme alpha-glucosidase is under cr in l. monocytogenes. here, we report the cloning and characterization from l. monocytogenes of an apparent ortholog of ccpa, which encodes an important mediator of cr in several low-g+c-content gram-positive bacteria. l. ... | 1998 | 9829942 |
| tolerance limits and methodology: effect on international trade. | microbiological contamination of foods with listeria monocytogenes, salmonella spp., campylobacter spp., and other pathogens and toxins and chemical and environmental contaminants can cause serious health and trade problems in the international trade of foods. consequently, a system of monitoring and surveillance of the quality and safety of imported foods can have a significant impact on food trade between two or more countries. the world trade organization (wto) provides a framework for ensuri ... | 1998 | 9829206 |
| behavior of listeria spp. in smoked fish products affected by liquid smoke, nacl concentration, and temperature. | the growth, survival, and death of listeria monocytogenes were studied in a synthetic medium as a function of temperature, nacl content, and amount of liquid smoke, and the findings were validated in smoked fish products. the smoke preservative compound was simulated by adding liquid smoke, and the concentration was expressed as phenol concentration. the growth of l. monocytogenes was limited at a temperature as low as 4 degrees c or at a phenol concentration as high as 20 ppm. the predicted val ... | 1998 | 9829188 |
| organic acid dipping of catfish fillets: effect on color, microbial load, and listeria monocytogenes. | microbiological and color changes of catfish fillets were determined following dip treatment in solutions at 4 degrees c of 2% acetic, citric, hydrochloric, lactic, malic, or tartaric acid. fillets were inoculated with an eight-strain mixture of listeria monocytogenes prior to dipping. l. monocytogenes, coliform, and aerobic plate counts and surface ph and hunter color were measured at 0, 2, 5, and 8 days of storage at 4 degrees c. acid dipping reduced surface ph and l. monocytogenes, coliform, ... | 1998 | 9829187 |
| phosphatidylcholine-specific phospholipase c from listeria monocytogenes is an important virulence factor in murine cerebral listeriosis. | meningoencephalitis is a serious and often fatal complication of listeria monocytogenes infection. the aim of the present study was to analyze the role of internalin a (inla) and b, which are involved in the invasion of l. monocytogenes into cultivated host tissue cells, and that of phosphatidylcholine-specific phospholipase c (plcb), which mainly promotes the direct cell-to-cell spread of l. monocytogenes, in murine cerebral listeriosis by use of an inla/b (deltainlab2)- and a plcb (deltaplcb2) ... | 1998 | 9826375 |
| interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with listeria monocytogenes in rats. | exogenous interleukin-15 (il-15) stimulates intestinal intraepithelial lymphocytes (i-iel) from mice to proliferate and produce gamma interferon (ifn-gamma) in vitro. to determine whether endogenous il-15 is involved in activation of i-iel during intestinal infection, we examined il-15 synthesis by intestinal epithelial cells (i-ec) after infection with listeria monocytogenes in rats. in in vitro experiments, invasion of l. monocytogenes into iec-6 cells, a rat small intestine epithelial cell li ... | 1998 | 9826341 |
| gamma delta t cells in infection-induced and autoimmune-induced testicular inflammation. | in a previous report, we investigated inflammatory responses induced by injecting listeria monocytogenes into one testis of a mouse. we demonstrated that the contralateral testis also developed an orchitis despite the absence of bacteria, indicating that the inflammation on the uninfected, contralateral side was of autoimmune character. in both infected and autoimmune testes, gammadelta and alphabeta t cells infiltrated during the inflammation. in this paper, we present the data of a comparison ... | 1998 | 9824503 |
| the role of gammadelta t cells in induction of bacterial antigen-specific protective cd8+ cytotoxic t cells in immune response against the intracellular bacteria listeria monocytogenes. | the role of t-cell receptor (tcr) gammadelta t cells in the induction of protective tcr alphabeta t cells against infection by the intracellular bacteria listeria monocytogenes was analysed. we found that depletion of gammadelta t cells by anti-tcr delta monoclonal antibody treatment before intravenous immunization of mice with a sublethal dose of viable l. monocytogenes resulted in reduction of protection against secondary challenge infection in the immunized mice. the gammadelta t-cell depleti ... | 1998 | 9824480 |
| intraoperative modulation of alveolar macrophage function during isoflurane and propofol anesthesia. | alveolar macrophages are a critical part of the defense against pulmonary infection. thus the authors determined time-dependent changes in alveolar macrophage functions in patients having surgery who were anesthetized with isoflurane or propofol. | 1998 | 9822000 |
| early ifn-gamma production and innate immunity during listeria monocytogenes infection in the absence of nk cells. | nk cells are believed to play a mandatory role during the early phases of listeria monocytogenes infection by producing ifn-gamma, which is required for the activation of macrophage effector functions. mice deficient in the common cytokine receptor gamma-chain (gamma(c)-/-), which completely lack nk cells, were used to examine whether nk cells were essential for resistance to listeria infection in vivo. surprisingly, infected gamma(c)-/- mice showed normal innate immunity and macrophage response ... | 1998 | 9820538 |
| optimization of codon usage of plasmid dna vaccine is required for the effective mhc class i-restricted t cell responses against an intracellular bacterium. | in an attempt to study codon usage effects of dna vaccines on the induction of mhc class i-restricted t cell responses against an intracellular bacterium, listeria monocytogenes, we designed two plasmid dna vaccines encoding an h-2kd-restricted epitope of listeriolysin o (llo) of l. monocytogenes, llo 91-99. one dna vaccine, p91wt, carries the wild-type dna sequence encoding llo 91-99, and the other one, p91mam, possesses the altered dna sequence in which the codon usage was optimized for murine ... | 1998 | 9820537 |
| sources and routes of contamination of raw milk with listeria monocytogenes and its control. | to identify the source of contamination of raw bulk milk with listeria, we attempted to isolate the bacteria from various samples such as cattle-related samples, bulk storage tanks and the environments on three farms. on farms a and b, listeria monocytogenes was repeatedly isolated from raw milk, while on farm c, it was scarcely isolated from it. on the former farms, listeria was detected in cattle-related samples and the environments. on the other hand, only one fecal sample on the latter farm ... | 1998 | 9819776 |
| neutrophil-kupffer-cell interaction in host defenses to systemic infections. | most relevant textbooks characterize phagocytosis by kupffer cells as the principal mechanism for clearing bacterial pathogens from the bloodstream and eliminating them from the liver. here, stephen gregory and edward wing discuss recent evidence indicating that the actual mechanism is far more complicated, dependent upon the complex interaction of kupffer cells with neutrophils that immigrate into the liver following infection. | 1998 | 9818544 |
| postnatally induced inactivation of gp130 in mice results in neurological, cardiac, hematopoietic, immunological, hepatic, and pulmonary defects. | the pleiotrophic but overlapping functions of the cytokine family that includes interleukin (il)-6, il-11, leukemia inhibitory factor, oncostatin m, ciliary neurotrophic factor, and cardiotrophin 1 are mediated by the cytokine receptor subunit gp130 as the common signal transducer. although mice lacking individual members of this family display only mild phenotypes, animals lacking gp130 are not viable. to assess the collective role of this cytokine family, we inducibly inactivated gp130 via cre ... | 1998 | 9815272 |
| listeria monocytogenes infection and activation of human brain microvascular endothelial cells. | listeria monocytogenes invasion of human brain microvascular endothelial cells (bmec) and its role as a stimulus for endothelial cell activation were studied. binding and invasion of intact bmec monolayers were independent of the l. monocytogenes inlab invasion locus. cytochalasin d abrogated invasion of bmec, whereas genistein effected only a 53% decrease in invasion, indicating a requirement for rearrangement of actin microfilaments but less dependence on tyrosine kinase activity. l. monocytog ... | 1998 | 9815218 |
| unidentified listeria-like bacteria isolated from cheese. | unidentified listeria-like bacteria, which lack only one of the phenotypic characteristics used to confirm listeria spp., were isolated from cheese during routine analysis for listeria monocytogenes. the vidas listeria assay and the listeria specific pcr or dna probe assays used did not identify these strains as listeria species. this group of bacteria was studied for its homogeneity using rep-pcr and pfge. sequence analysis of the 16s rrna gene showed a homology of 94% to established listeria s ... | 1998 | 9812394 |
| use of bacteriocinogenic lactic acid bacteria to inhibit spontaneous nisin-resistant mutants of listeria monocytogenes scott a. | nisin is a bacteriocin with a broad antibacterial spectrum including strains of listeria monocytogenes. populations of l. monocytogenes, however, frequently contain spontaneous nisin-resistant mutants. when a culture of l. monocytogenes scott a was exposed to nisin concentrations between 10 and 500 iu ml-1, the initial decrease in viable numbers was followed by regrowth of survivors to nisin. nisin-resistant mutants of l. monocytogenes scott a were isolated after a single exposure to nisin at 10 ... | 1998 | 9812378 |
| protein antimicrobial barriers to bacterial adhesion. | the ability of microorganisms to adhere to solid surfaces is a problem of high visibility and has been the focus of numerous investigations because these organisms can cause disease and food spoilage. during the last several years, considerable attention has been focused on the development of food-grade antimicrobial barriers to adhesion in order to inhibit the initial adhesion of microbial contaminants by application of an antimicrobial agent to the surface rather than trying to remove these co ... | 1998 | 9812283 |
| biofilms in food processing environments. | biofilms are a constant concern in food processing environments. our overall research focus has been to understand the interaction of factors affecting bacterial attachment and biofilm formation with the ultimate goal of devising strategies to control this problem. this paper briefly describes three areas of biofilm research in which we have been involved. listeria monocytogenes, a foodborne pathogen, survived for prolonged periods on stainless steel and buna-n rubber, materials commonly used in ... | 1998 | 9812282 |
| correlation between production of listeriolysin o by variants of listeria monocytogenes and their virulence for rabbits. | l. monocytogenes serovar 1/2a nctc 7973 was passaged through rabbits and the severity of infection at each passage was determined by counting viable bacteria from infested organs and recording the time of death. a comparative evaluation of the levels of hemolysin produced in vitro by the original and six variant cultures (v1-v6) was done by determination of hemolytic units (chu). while virulence of the cultures enhanced at each passage (2.2 x 10(9) cfu/g of the spleen for v6 as compared to 5.0 x ... | 1995 | 9810660 |
| simplified purification of listeria monocytogenes listeriolysin o and preliminary application in the enzyme-linked immunosorbent assay (elisa). | a simplified procedure was developed for purification of listeriolysin o (llo) of listeria monocytogenes, consisting of hydroxylapatite adsorption chromatography followed by sepharose s ion exchange chromatography. the llo (58 kda) appeared pure in terms of sodium dodecylsulfate polyacrylamide electrophoresis and immunoblots with polyclonal rabbit immune sera. the purified llo could be stored at -65 degrees c for 1 year without loss of immunoreactivity. similarly, flat-bottom microtiter strips f ... | 1995 | 9810643 |
| interferon gamma derived from cd4(+) t cells is sufficient to mediate t helper cell type 1 development. | interferon gamma (ifn-gamma) has been implicated in t helper type 1 (th1) cell development through its ability to optimize interleukin 12 (il-12) production from macrophages and il-12 receptor expression on activated t cells. various systems have suggested a role for ifn-gamma derived from the innate immune system, particularly natural killer (nk) cells, in mediating th1 differentiation in vivo. we tested this requirement by reconstituting t cell and ifn-gamma doubly deficient mice with wild-typ ... | 1998 | 9802977 |
| a small outbreak of listeriosis associated with smoked mussels. | two perinatal listeriosis cases in auckland, new zealand, which were diagnosed during november and december 1992 gave histories of consuming brand x smoked mussels. listeria monocytogenes was isolated from an unopened packet of mussels collected from the refrigerator of one of the cases. cultures of l. monocytogenes from the two patients were indistinguishable from those recovered from the mussels by serogrouping and dna macrorestriction analysis using pulsed-field gel electrophoresis (pfge). pf ... | 1998 | 9801198 |
| susceptibility of antibiotic-resistant and antibiotic-sensitive foodborne pathogens to acid anionic sanitizers. | acid anionic sanitizers for treatment of fruits and vegetables were prepared using ingredients generally recognized as safe by the u.s. food and drug administration or anionic surfactants and organic acid food additives. they met the regulatory definition as sanitizers by showing bactericidal efficacy of 99.999% in 30 s against staphylococcus aureus atcc 6538 and escherichia coli atcc 11229. these sanitizers showed a broad spectrum of microbicidal activity against both gram-positive and gram-neg ... | 1998 | 9798163 |
| antibiotic resistance of salmonella spp. and listeria spp. isolates from traditionally made fresh sausages in greece. | sixty-five samples of traditionally made fresh sausages obtained from retail shops and butcher shops in northern greece were screened for the presence of salmonella spp. and listeria spp. salmonella spp. were found in 20% of the samples tested (54% salmonella typhimurium and 46% salmonella enteritidis). the prevalence of listeria spp. in the samples was 26% (12% listeria monocytogenes, 76% listeria innocua, and 12% listeria welshimeri). nine of 13 salmonella isolates were found to be resistant t ... | 1998 | 9798159 |
| efficacy of spray application of chlorinated water in killing pathogenic bacteria on raw apples, tomatoes, and lettuce. | washing whole and cut produce by dipping or submerging in chlorinated water has a sanitizing effect, although reduction in microbial populations is minimal and is usually less than 100-fold. a study was undertaken to evaluate the efficacy of a spray application of chlorine in killing salmonella, escherichia coli o157:h7, listeria monocytogenes, yeasts and molds, and total aerobic mesophilic microorganisms on whole apples, tomatoes, and lettuce leaves. inoculated produce was treated (sprayed and ... | 1998 | 9798146 |
| the contamination of pâté with listeria monocytogenes--results from the 1994 european community-coordinated food control program for england and wales. | the listeria monocytogenes contamination of 3,065 pâté products sampled at the point of retail sale in england and wales was examined. ninety-seven percent of samples were free of contamination with l. monocytogenes, 2.0% (60) had levels of less than 200 cfu/g, and 0.6% (18) had levels of 200 cfu/g or more. fish and seafood pâté were significantly more commonly contaminated by l. monocytogenes than other pâté types (chi 2 test, p = 0.001). pâté obtained from small retail shops was significantly ... | 1998 | 9798145 |
| effect of environmental stress on the ability of listeria monocytogenes scott a to attach to food contact surfaces. | attachment of listeria monocytogenes scott a to buna-n rubber and stainless steel under different temperature and ph conditions at the time of cell growth or at the time of attachment was investigated. all experiments were conducted using sterile phosphate buffer to avoid cell growth during exposure to the test surfaces. numbers of attached cells increased with increasing attachment temperature (10 to 45 degrees c) and exposure time for both test surfaces. maximum levels of attached cells were o ... | 1998 | 9798144 |
| influence of environmental stress on the kinetics and strength of attachment of listeria monocytogenes scott a to buna-n rubber and stainless steel. | attachment and detachment of listeria monocytogenes scott a to buna-n rubber and stainless steel under varying conditions of temperature and ph were investigated using model systems. numbers of attached cells increased with increasing attachment temperature (10 to 45 degrees c) and time (up to 120 min) for both test surfaces. compared to buna-n rubber, the rate of attachment to stainless steel was markedly more rapid for all temperature and ph conditions studied and could not be calculated. rate ... | 1998 | 9798143 |
| antimicrobial effects of d-3-phenyllactic acid on listeria monocytogenes in tsb-ye medium, milk, and cheese. | d-3-phenyllactic acid is a compound with anti-listeria activity which is produced and secreted by the yeastlike fungus, geotrichum candidum. this compound has a bactericidal effect independent of the physiological state of listeria monocytogenes when added at a concentration of 7 mg/ml to tryptic soy broth supplemented with yeast extract (tsb-ye). an initial l. monocytogenes population of 10(5) cfu/ml was reduced 100-fold (2 log) after 4 days of culture at 25 degrees c in tsb-ye containing d-3-p ... | 1998 | 9798142 |
| the effect of reuterin on listeria monocytogenes and escherichia coli o157:h7 in milk and cottage cheese. | a broad-spectrum reuterin produced during anaerobic fermentation of glycerol by lactobacillus reuteri strain 12002 was found to be inhibitory and bactericidal for listeria monocytogenes and escherichia coli o157:h7. lyophilized reuterin was prepared by a two-step fermentation process. a batch fermentation in a 15-liter fermentor was applied to produce a maximum biomass of l. reuteri using a modified mrs broth at ph 4.3. further, harvested cells were used to ferment glycerol (250 mm) under anaero ... | 1998 | 9798141 |
| [infectious aneurysm due to listeria monocytogenes: a new case and review of the literature]. | infections due to listeria monocytogenes usually occur in pregnant women, in the elderly and in immunocompromised patients. arterial aneurysms due to this germ are rare. only 16 cases have been previously described in the literature. | 1998 | 9793154 |
| a novel prfa-regulated chromosomal locus, which is specific for listeria ivanovii, encodes two small, secreted internalins and contributes to virulence in mice. | several large, cell wall-associated internalins and one small, secreted internalin (inlc) have been described previously in listeria monocytogenes. using degenerate primers derived from sequenced peptides of an l. ivanovii major secreted protein, we identified a new 4.25 kb internalin locus of l. ivanovii, termed i-inlfe. the two proteins encoded by this locus, i-inle and i-inlf, belong to the group of small, secreted internalins. southern blot analyses show that the i-inlfe locus does not occur ... | 1998 | 9791184 |
| occurrence of listeria monocytogenes in sewage sludge. | the search for salmonella is the only bacteriological index included in italian guidelines (in accordance with eu regulations) for the use of sewage sludge in agriculture. as a result, information regarding the presence of listeria monocytogenes is rather limited. we therefore decided to carry out an investigation of listeria in the sludge produced by the bologna (italy) treatment plant during the various phases of treatment. five different types of sludge were analysed (primary raw, activated, ... | 1998 | 9789361 |
| perinatal listeriosis: a population-based multicenter study in barcelona, spain (1990-1996). | the aim off this study was to describe the incidence, epidemiology, clinical presentation, and outcome of perinatal listeriosis for a 7-year period (1990-1996) based on data of an active population-based surveillance project implemented in the city of barcelona, spain. there were 30 cases (20.8%) associated with pregnancy (15 pregnant women, 13 neonates, and 2 fetal deaths). the incidence of perinatal listeriosis varied from 4.1 to 0 per 10,000 live births. the proportion of perinatal cases in r ... | 1998 | 9788644 |
| detection of listeria in raw and pasteurized milk. | one hundred raw milk samples from different regions of anatolia and 20 pasteurized milk samples from three different manufacturers in ankara were analyzed for the presence of listeria spp. l. monocytogenes was found in 1% of the raw milk samples and in 5% (1/20) of the pasteurized milk samples. l. innocua and l. seeligeri were found in 8 and 2% of the raw milk samples, respectively. no other species of listeria was found. the overall incidence of listeria spp. was 10% in the raw milk samples and ... | 1998 | 9787931 |
| in-vitro activity of the new ketolide antibiotic hmr 3647 against gram-positive bacteria. | the comparative in-vitro activity of hmr 3647, a new ketolide antibiotic, was investigated against 492 clinical isolates of gram-positive bacteria, including multiply resistant strains, by an agar-dilution technique. all streptococci tested were inhibited by the new ketolide at concentrations < or = 0.5 mg/l. hmr 3647 was more potent than erythromycin against staphylococci. for enterococci the new compound yielded an mic90 of 8 mg/l. erysipelothrix spp., pediococcus spp., leuconostoc spp., lacto ... | 1998 | 9786468 |
| immunohistochemical detection of listeria antigens in the placenta in perinatal listeriosis. | listeria monocytogenes, a worldwide pathogen, causes significant perinatal mortality and morbidity and has been implicated in spontaneous abortions, still-births, premature delivery, and neonatal sepsis, often with meningitis. maternal symptoms are frequently minimal, and diagnosis is made only if the suspicion is high and diagnostic maternal blood or amniotic fluid cultures are performed. because cultures are not routinely performed on spontaneously aborted fetuses, many authors feel that the t ... | 1998 | 9785135 |
| interaction of listeria monocytogenes with human brain microvascular endothelial cells: inlb-dependent invasion, long-term intracellular growth, and spread from macrophages to endothelial cells. | invasion of endothelial tissues may be crucial in a listeria monocytogenes infection leading to meningitis and/or encephalitis. internalization of l. monocytogenes into endothelial cells has been previously demonstrated by using human umbilical vein endothelial cells as a model system. however, during the crossing of the blood-brain barrier, l. monocytogenes most likely encounters brain microvascular endothelial cells which are strikingly different from macrovascular or umbilical vein endothelia ... | 1998 | 9784531 |
| [the persistent characteristics of listeria monocytogenes isolated from different sources]. | a number of biological properties of l. monocytogenes was studied. significant differences in the lysozyme, "anti-interferon", rnaase and lipase activity between strains isolated from different warm-blooded animals and from environmental objects were shown. 75% of all strains under study were found to have "anti-interferon" activity, while no antilysozyme activity was detected. | 1998 | 9783394 |
| [newborn infant bacterial infections. diagnosis, treatment principles, prevention]. | 1998 | 9781160 | |
| use of the protective antigen of erysipelothrix rhusiopathiae in the enzyme-linked immunosorbent assay and latex agglutination. | to establish a safe and convenient serodiagnostic method for swine erysipelas, a purified protective protein antigen of erysipelothrix rhusiopathiae, which included a large amount of protective protein (64 kda protein), was used for enzyme-linked immunosorbent assay (elisa) and the latex agglutination (la) test. in the elisa, the antisera to four different serovars (1a, 2, 5 and 20) of e. rhusiopathiae exhibit a positive reaction, while antisera to other species of bacteria (listeria monocytogen ... | 1998 | 9780828 |
| heat-killed listeria monocytogenes as an adjuvant converts established murine th2-dominated immune responses into th1-dominated responses. | we investigated the capacity of heat-killed listeria monocytogenes (hkl), a potent stimulator of the innate immune system, as a vaccine adjuvant to modify both primary and secondary ag-specific immune responses. mice immunized with the ag keyhole limpet hemocyanin (klh) mixed with hkl generated a klh-specific primary response characterized by production of th1 cytokines and large quantities of klh-specific igg2a ab. moreover, administration of klh with hkl as an adjuvant reversed established imm ... | 1998 | 9780187 |
| studying m cells and their role in infection. | in addition to sampling antigens, m cells are a common route for pathogen invasion. recent studies have partly defined the mechanisms by which pathogens interact with and exploit m cells as a gateway into the host. new research tools are facilitating studies on m cell infection, differentiation and function. | 1998 | 9778729 |
| bone marrow cellular composition in listeria monocytogenes infected mice detected using er-mp12 and er-mp20 antibodies: a flow cytometric alternative to differential counting. | detailed assessment of bone marrow cellular composition is essential in the evaluation of various experimental in vivo systems, such as expression of transgenes, null mutations and stimulation of host defence in infection. traditional morphological analysis of mouse bone marrow is laborious, requires specific cytological expertise, and is somewhat subjective. as an alternative, we have examined whether double labelling of bone marrow with the anti-precursor monoclonal antibodies er-mp12 and er-m ... | 1998 | 9776572 |
| [listeria monocytogenes: a rare cause of spontaneous bacterial peritonitis in liver cirrhosis]. | 1998 | 9775176 | |
| central nervous system infection with listeria monocytogenes. 33 years' experience at a general hospital and review of 776 episodes from the literature. | we reviewed 776 previously reported and 44 new cases of cns listeriosis outside of pregnancy and the neonatal period, and evaluated the epidemiologic, diagnostic, and therapeutic characteristics of this infection. among patients with listeria meningitis/meningoencephalitis, hematologic malignancy and kidney transplantation were the leading predisposing factors, but 36% of patients had no underlying diseases recognized. the infection occurred throughout life, with a higher incidence before the ag ... | 1998 | 9772921 |
| effects of prolonged exposure to morphine and methadone on in vivo parameters of immune function in rats. | in rats, two 6-week repeated dose oral toxicity studies were performed with morphine (250 and 500 mg/kg food) and methadone (200 and 400 mg/kg food), respectively. alterations in immune function were studied by assessing primary and secondary immune responses to sheep red blood cells. in addition, the ability to resist challenge with infectious agents was measured in host resistance models employing the parasite trichinella spiralis and the bacterium listeria monocytogenes. the primary and secon ... | 1998 | 9772098 |