Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antiviral therapy with small particle aerosols. | the generation and use of small particle aqueous aerosols (1.23 microns aerodynamic mass median diameter, gsd = 2.0 microns) containing ribavirin is described. administered via aerosol, ribavirin will be deposited rather uniformly on the surface of the nasopharynx, the tracheobronchial tree and in the pulmonary area. examples of aerosol-delivered dosages found to be effective in the treatment of respiratory syncytial virus infection and influenza a and b virus infections are as follows: 12.8 mg ... | 1988 | 2850911 |
| peptides as potential virus inhibitors. synthesis and bioassay of five respiratory syncytial virus peptide analogs with antimeasles activity. | five carbobenzoxylated and d-amino acid containing-peptide analogs of the respiratory syncytial virus (rsv) f1 glycoprotein amino terminus were chemically synthesized by solution and fmoc-solid phase peptide synthesis methods. several of these peptides, ranging from 3 to 6 residues in length, raised the bilayer to hexagonal phase transition temperature of dielaidoylphosphatidylethanolamine. none of these peptides were specific inhibitors of rsv or herpes simplex virus infection. two of the serie ... | 1988 | 2850279 |
| serologic study of febrile upper respiratory tract infections in adults. | we prospectively studied serum antiviral antibody response in adult patients with febrile upper respiratory tract infections. paired sera were obtained in 158 patients from january 1982 to december 1983. eighteen patients (11.4 percent) showed a significant antibody rise in the convalescent phase of the disease compared to the acute phase. significant antibody rise was observed against all viruses examined: influenza viruses type a and b, adenovirus type 3, respiratory syncytial virus, and parai ... | 1988 | 2848145 |
| upper respiratory tract infection and serum antibody responses in nursing home patients. | residents of a veterans administration nursing home care unit (nhcu) were observed for the development of upper respiratory tract infection (uri) during 12 consecutive months to determine the frequency of sporadic cases or outbreaks of uri and to characterize them clinically and by laboratory means. fifty-nine episodes of uri occurred in 56 residents during the study period. serologic testing or virus isolation proved or suggested an etiologic agent on 22 occasions. uri was more common in late f ... | 1988 | 2847597 |
| serologic survey of selected pathogens in white-tailed and mule deer in western nebraska. | exposure of free-ranging white-tailed deer (odocoileus virginianus) and mule deer (odocoileus hemionus) in western nebraska to selected livestock pathogens was determined by serology and attempted virus isolation. antibodies to bluetongue virus, epizootic hemorrhagic disease virus, and bovine respiratory syncytial virus were present in both species of deer. no serologic reactors to brucella or anaplasma were found. attempts to isolate bluetongue virus were negative. | 1986 | 2845156 |
| contribution of rhinoviruses to respiratory viral infections in childhood: a prospective study in a mainly hospitalized infant population. | a prospective study was carried out to investigate the contribution of rhinoviruses to respiratory viral infections in children and to investigate the influence of age, passive smoking, and educational level of the head of the family on the clinical course of viral respiratory disease. nasopharyngeal aspirates from 519 infants (90.8% inpatients, 9.2% outpatients) were screened for the presence of rhinoviruses, respiratory syncytial virus (rsv), adenoviruses, parainfluenza virus types 1, 2, 3, in ... | 1988 | 2844986 |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| current approaches to the development of vaccines effective against parainfluenza viruses. | infection of infants and children with parainfluenza viruses can lead to serious lower respiratory tract disease. in order to review new information on the genetic organization, epidemiology, and immunobiology of these viruses and of respiratory syncytial virus, who, in conjunction with the national institute of allergy and infectious diseases, held a workshop in bethesda, md, usa, 4-5 may 1987. new data on the immunobiology and epidemiology of human parainfluenza 3 virus were presented that sho ... | 1988 | 2844427 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| mycoplasma pneumoniae and other pathogens in the aetiology of lower respiratory tract infections among japanese children. | the causes of lower respiratory tract infections in 1544 children attending a japanese hospital over a period of 7 years were investigated. both cultivation and two serological techniques were used to detect mycoplasma pneumoniae whereas viral involvement was investigated only by serology. pathogens were identified in 52% of 1175 patients with pneumonia and 36% of 369 patients without pneumonia. mycoplasma pneumoniae infection was specifically diagnosed in 414 (26.8%) of the 1544 patients. respi ... | 1988 | 2840466 |
| simultaneous infection with respiratory syncytial virus and other respiratory pathogens. | the presentation and subsequent course of respiratory syncytial virus (rsv) bronchiolitis may be atypical and unusually severe when simultaneous infection due to other pathogenic agents is present. during the past two years, nine of the 189 pediatric patients hospitalized with documented rsv infection were found to have the following simultaneous isolates from initial respiratory tract specimens: four adenovirus, four pneumococcus, one cytomegalovirus, and one pneumocystis carinii. noted complic ... | 1988 | 2839976 |
| live viral vaccines for respiratory and enteric tract diseases. | in its programme for accelerated development of vaccines for viral respiratory and enteric tract diseases the who has assigned a very high priority to respiratory syncytial virus (rsv), parainfluenza viruses and rotaviruses. there is also some interest in alternative approaches to immunization against influenza viruses because of the failure of inactivated vaccines to provide complete and reasonably durable immunity. current attempts to develop satisfactorily attenuated viruses for use in preven ... | 1988 | 2838984 |
| use of a-549 cells in a clinical virology laboratory. | a-549 cells were compared with other cell lines for virus recovery, except from specimens submitted specifically for detection of cytomegalovirus. of 589 specimens submitted specifically for detection of herpes simplex virus (hsv), 163 (28%) were positive for hsv--159 (97.5%) in a-549 cells and 156 (96%) in primary rabbit kidney cells. hsv cytopathic effect was identified an average of 0.6 day earlier in a-549 cells. virus was recovered from 194 (11%) of 1,790 specimens submitted for general vir ... | 1988 | 2838502 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| participation of cytoskeletal intermediate filaments in the infectious cycle of human respiratory syncytial virus (rsv). | rsv infection of hep-2 or hela cells leads to biochemical and morphological changes of cytoskeletal intermediate filaments (if). thus, human cytokeratin 18 is modified to generate a more acidic polypeptide of slightly larger apparent molecular weight. in addition, the amounts of vimentin and other cytokeratins are reduced, probably as a consequence of proteolytic degradation. these changes are reflected in a decrease of immunofluorescence with specific antibodies in rsv-induced syncytia and a mo ... | 1988 | 2837016 |
| detection of respiratory syncytial virus and rotavirus by enhanced chemiluminescence enzyme-linked immunosorbent assay. | the horseradish peroxidase-catalyzed oxidation of luminol was used in a chemiluminescence avidin-biotin enzyme-linked immunosorbent assay (cl-abe) to detect respiratory syncytial virus (rsv) and rotavirus (rov). this cl-abe was carried out in a new apparatus constructed for the photographic registration of the light emission produced. when measured in a spectrophotometer, the light emission produced by the oxidation of luminol showed a peak emission at 425 nm. the chemiluminescence output reache ... | 1988 | 2835387 |
| viral "bronchitis" in childhood: relationship to asthma and obstructive lung disease. | lower respiratory tract infections in children, including group, bronchiolitis, and bronchitis are frequently associated with recurrent episodes of wheezing. different respiratory viruses assume greater importance at different ages of children. respiratory syncytial virus is the most prevalent viral respiratory infection in preschool children, while rhinovirus is of increasing importance in older children. asymptomatic virus shedding and mild respiratory infections do not provoke asthma symptoms ... | 1988 | 2834808 |
| serological investigations for some bacterial and viral pathogens in fallow deer (cervus dama) and wild boar (sus scrofa) of the san rossore preserve, tuscany, italy. | sera of 43 fallow deer (cervus dama) of the san rossore preserve (tuscany, italy) were examined for antibodies against eight pathogens; one proved positive for brucella sp., 21 for listeria monocytogenes, 34 for chlamydia psittaci, three for coxiella burnetii, one for infectious bovine rhinotracheitis virus, 11 for parainfluenza-3 virus, 25 for bovine viral diarrhea virus and six for bovine respiratory syncytial virus. no age and sex difference in the positivity rates and titers was evidenced, w ... | 1988 | 2832622 |
| viral infections in pediatric bone marrow transplant patients. | the records of 96 pediatric patients with aplastic anemia or a malignancy who underwent bone marrow transplantation between 1979 and 1986 at the children's hospital of philadelphia were reviewed for laboratory evidence of viral infections. the most common viral diseases identified were herpes simplex virus (hsv), cytomegalovirus and adenoviruses, which were found in 19 (20%), 17 (18) and 17 (18) patients, respectively. hsv was more common in patients with than without graft vs. host disease (gvh ... | 1988 | 2830586 |
| roentgenographic features of common pediatric viral respiratory tract infections. | viral infections of the respiratory tract in infants and children are common. little has been reported on roentgenographic findings associated with infection caused by common viruses other than with respiratory syncytial virus. we studied chest roentgenograms from 128 previously healthy infants and children who were infected with respiratory syncytial virus, parainfluenza virus, influenza virus, or adenovirus. four common roentgenographic findings were detected: parahilar peribronchial infiltrat ... | 1988 | 2829619 |
| protection against respiratory disease in calves induced by vaccines containing respiratory syncytial virus, parainfluenza type 3 virus, mycoplasma bovis and m dispar. | a field trial to assess the ability of two vaccines to protect calves against respiratory disease was carried out on a large beef rearing unit in southern england over the two winters of 1983 to 1984 and 1984 to 1985. a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma bovis and m dispar or a vaccine containing only the respiratory syncytial virus component were inoculated into 246 and 245 calves, respectively; 245 calves r ... | 1987 | 2827366 |
| [infectious respiratory tract diseases in cattle in the winter of 1985/86: detection of infection by bovine respiratory syncytial virus and parainfluenza virus type 3 by immunofluorescence in nasal swab samples and by antibody titer increase in serum (elisa)]. | 1987 | 2827303 | |
| pulmonary infections with respiratory syncytial virus and the parainfluenza viruses. | respiratory syncytial virus (rsv) and the parainfluenza viruses (pivs) are the most important causes of acute lower respiratory illness (lri) in infants and children under 6 years of age. these enveloped viruses are members of the paramyxovirus family. they infect cells in the epithelium lining the trachea and intrapulmonary airways, and cause croup, bronchitis, bronchiolitis, and bronchopneumonia. rsv causes annual midwinter to early spring outbreaks of respiratory disease in temperate climates ... | 1987 | 2827280 |
| hospital-acquired pneumonia in children: the role of respiratory viruses. | the most frequent agents of pneumonia acquired by children in the hospital are viruses. the characteristics of these nosocomial viral agents differ appreciably from the more classical bacterial nosocomial infections. the viral agents tend to be more contagious, with normal children being as susceptible as the predisposed, high-risk child. the epidemiology of these hospital-acquired infections tends to mimic the patterns of activity of the respiratory viruses in the community. the major causes of ... | 1987 | 2827278 |
| epidemiology of viral infections in singapore. | as singapore is a densely populated island, and also a major air and sea port, the importation and dissemination of viral infections is facilitated. respiratory viral infections have the highest prevalence rates, influenza and respiratory syncytial virus (rsv) being the most important ones. seasonal variation occurs with influenza, rsv and parainfluenza virus type 1 infections. the age distribution and clinical picture associated with infections due to the various respiratory viruses are similar ... | 1987 | 2825585 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| cryogenic preservation of virus-infected cells used as immunofluorescent assay substrates. | cells infected with herpes simplex virus, respiratory syncytial virus and adenovirus were subjected to several cryogenic regimens to determine whether these procedures were detrimental to the performance and results obtained with an indirect immunofluorescence assay (ifa). infected cells were harvested and divided into equal aliquots prior to freezing at different temperatures (-20, -70 and -165 degrees c). cells fixed onto glass slides immediately after harvesting were used as controls. for the ... | 1987 | 2824546 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on virus interference. | during the 7-year period from september 1978 to august 1985, smear specimens of nasopharyngeal secretions from 3132 patients mainly hospitalized children, taken in different regions in norway, were examined for respiratory viruses by the rapid immunofluorescence (if) technique. a positive diagnosis for respiratory syncytial virus (rsv), parainfluenza virus type 1, 2 and 3 or influenza a and b virus was made for 896 patients (29%). the greatest prevalence for all these viruses was observed during ... | 1987 | 2824225 |
| respiratory syncytial virus infections in children admitted to the intensive care unit. | during the winter of 1986-1987, 64 children with respiratory syncytial virus (rsv) infection were admitted to our hospital. the diagnosis was made by direct immunofluorescent antibody technique. twenty-three children (36%) needed intensive care treatment. nearly 11 (52%) had a preexisting disease state, identified as a risk factor i.e., prematurity (n = 8), bronchopulmonary dysplasia (n = 2), congenital heart disease (n = 1). twelve patients (50%) were intubated and ventilated. conditions for in ... | 1989 | 2812976 |
| bacterial coinfection in children hospitalized with respiratory syncytial virus infections. | clinical and bacterial findings were prospectively studied in 90 children hospitalized because of middle or lower respiratory tract infection caused by respiratory syncytial virus (rsv) during a surveillance period of 12 months. the results were compared with those of rsv-negative children hospitalized with identical indications during the 3 peak months of the rsv epidemic (n = 91) or for the 3 months after the outbreak (n = 99). a high frequency of pneumonia and acute otitis media were found in ... | 1989 | 2812913 |
| antibody-mediated enhancement of respiratory syncytial virus infection in two monocyte/macrophage cell lines. | monoclonal antibodies (mabs) specific for two surface glycoproteins of respiratory syncytial virus (rsv) were found to enhance rsv infection in two macrophagelike cell lines (p388d1 and thp-1). mabs to an irrelevant antigen (pneumococcal polysaccharide) and to the nucleocapsid of rsv did not enhance infection. blocking either the fc segment of the monoclonal antibody of the fc receptor on the cells diminished the enhancement, suggesting that this phenomenon involves attachment of the monoclonal ... | 1989 | 2809253 |
| hilar enlargement in respiratory syncytial virus pneumonia. | the clinical and radiographic features of ten children with hilar enlargement in association with proven respiratory syncytial virus (rsv) infection are described. hilar enlargement was seen in 10/35 children with rsv infection, and was invariably unilateral and right sided. it is recommended that rsv pneumonia be considered in children with unilateral hilar enlargement if tuberculosis has been excluded, and the onset of disease is rapid. | 1989 | 2806272 |
| comparison of caprine, human and bovine strains of respiratory syncytial virus. | a new continuous ovine kidney cell line allowing the growth of caprine, human and bovine respiratory syncytial virus was used to minimize host cell related variations for the direct comparison of the viral ultrastructures, serological relationships and structural protein profiles. results show that all three strains are closely related although a closer relationship was found between bovine and caprine rs. | 1989 | 2803001 |
| activation of complement by bovine respiratory syncytial virus-infected cells. | because complement activation is probably involved in the pathogenesis of as well as in recovery from the disease induced by bovine respiratory syncytial virus (brsv), we studied the activation of complement by brsv-infected cells in vitro in a homologous system. binding of c3 on the surface of infected cells was measured in a biotin-streptavidin amplified elisa, and complement-mediated lysis was measured in a 51cr release assay. without antibody, infected cells activated and bound more c3 than ... | 1989 | 2800328 |
| disturbance in respiratory mechanics in infants with bronchiolitis. | the passive flow-volume and partial forced expiratory flow-volume techniques were used to assess pulmonary function in 14 spontaneously breathing infants with acute respiratory syncytial virus bronchiolitis. two additional infants were studied while paralysed and ventilated. during the acute stage of the illness there was a significant reduction in forced expiratory flow rates and an increase in respiratory resistance. although the mean thoracic gas volume for the group was increased, five infan ... | 1989 | 2799745 |
| effect of vaccination on cell populations in lung washes from calves after infection with respiratory syncytial virus. | the inflammatory response in the air-passages of the lungs of calves after intranasal inoculation with respiratory syncytial virus (rsv) was compared in rsv-vaccinated and control animals. total cells recovered from lung washings remained the same; however, the fold by eight days after infection and the type of cells changed from a predominance (85 per cent) of macrophages to equal proportions of macrophages and neutrophils (45 per cent) during the course of infection. the absolute numbers of ne ... | 1989 | 2799080 |
| duration of hospitalization in previously well infants with respiratory syncytial virus infection. | to describe the typical hospital course of infection in previously well infants hospitalized with respiratory syncytial virus (rsv) infection, we reviewed the charts of 196 patients with laboratory-proved respiratory syncytial virus infection in the 1987-1988 respiratory disease season. eighty-seven of the children had been previously well. their mean duration of hospitalization was 3.4 days. previously well infants younger than 6 weeks of age experienced significantly longer hospitalizations an ... | 1989 | 2797955 |
| clinical characteristics of respiratory syncytial virus infections in healthy versus previously compromised host. | in an effort to delineate the clinical characteristics of respiratory syncytial virus (rsv) infection in the compromised host, we compared children with bronchopulmonary dysplasia (bpd), congenital heart disease (chd), premature birth, failure to thrive, and gastroesophageal reflux to previously healthy children. during a four-year period, 262 patients were admitted to the hospital with rsv infection diagnosed by a rapid rsv antigen detection test. children with bpd or chd had more hospital days ... | 1989 | 2797931 |
| childhood asthma following hospitalization with acute viral bronchiolitis in infancy. | a prospective follow-up of 48 infants hospitalized with respiratory syncytial virus bronchiolitis in the first year of life revealed that 44 of these infants had symptoms suggestive of asthma in the 5 years following their initial illness (cumulative prevalence 92%). symptoms became less frequent and less troublesome during the follow-up period. thirty-five of these children visited the laboratory for clinical examination, pulmonary function testing, and histamine challenge. twenty-five children ... | 1989 | 2797929 |
| [the role of cellular immunity in the etiopathogenesis of obstructive bronchitis in childhood]. | the development and aetiopathogenetic role of the immune response were studied in group of infants and children aged 4-6 years by comparison of clinical status, concentrations of serum immunoglobulins, virological tests (levels of specific antibodies and cultivation of viruses) and the macrophage migration inhibition test. a respiratory syncytial virus was found in 44% of virologically positive children. the macrophage migration inhibition test was positive in 45% of infants suffering from obstr ... | 1989 | 2791084 |
| interleukin-1 inhibitor production by human mononuclear leukocytes and leukocyte subpopulations exposed to respiratory syncytial virus: analysis and comparison with the response to influenza virus. | net interleukin-1 (il-1) inhibitor activity is induced by exposure of purified human monocytes-macrophages to respiratory syncytial virus (rsv). furthermore, il-1 inhibitor activity was produced by monocytes-macrophages exposed to rsv in the presence of lymphocytes, that is, by unseparated mononuclear leukocytes (mnl). purified rsv-exposed lymphocytes, as well as the lymphocytes exposed within mnl preparations, produced net il-1 inhibitor activity. in contrast, net il-1 activity was produced whe ... | 1989 | 2788203 |
| pathogenesis of naturally acquired bovine respiratory syncytial virus infection in calves: evidence for the involvement of complement and mast cell mediators. | indicators of immune-mediated disease were studied in calves with severe natural bovine respiratory syncytial virus infection. although antigen and antibody were detected concurrently in most calves, immune complexes were not detected by use of immunofluorescence, elisa, and binding of the 1q component of complement. complement component c3, however, was observed by immunofluorescence in the cranioventral, virus-infected portion of the lungs of 19 of 25 calves. reductions in the amount of histam ... | 1989 | 2786355 |
| flow cytometric analysis of pulmonary lymphocytes from mice infected with respiratory syncytial virus. | balb/c mice were infected intra-nasally with respiratory syncytial virus (rsv) and cells recovered from the lungs by single or repeated bronchoalveolar lavage (bal). single bal gave enough cells (1 - 2 x 10(5) cells/mouse) for morphological study of giemsa stained cytospin preparations, and allowed the same mouse lungs to be assayed for virus titre or processed for histology. in normal or sham-infected mice the majority of recovered cells were macrophages, with less than 5% lymphocytes. followin ... | 1989 | 2784741 |
| prostaglandin and thromboxane concentrations in plasma and lung lavage fluids during sequential infection of vaccinated and nonvaccinated calves with bovine respiratory syncytial virus. | the potential action of immunologic reactions and mediators released during the course of bovine respiratory syncytial virus infection in pathogenesis of the ensuing disease process was examined in an experimental infection study. prostaglandin (pg) e2, pgf2 alpha 6-keto-pgf1 alpha, and thromboxane b2 (txb2) concentrations were quantitated in plasma and lung lavage fluid by radioimmunoassay at 3- to 4-day intervals during a primary and secondary virus infection of vaccinated, nonvaccinated, and ... | 1989 | 2782709 |
| evaluation of exposure of health care personnel to ribavirin. | reports that ribavirin was teratogenic in animals raised concerns of female health care personnel about possible occupational exposure during the care of infants having respiratory syncytial virus infections. under simulated operational conditions, experiments were conducted to measure ribavirin residues in room air, in surface wipe samples, and in personal sampling devices worn by volunteers. there was exposure to a dispersible dust, presumably dried ribavirin, deposited inside the croupette or ... | 1989 | 2778845 |
| immunoglobulin a antibody response to respiratory syncytial virus structural proteins in colostrum and milk. | immunoglobulin a (iga) antibody response to respiratory syncytial virus (rsv) structural proteins in colostrum and milk was investigated by a radioimmunoprecipitation assay. by using [35s]methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody, iga antibody responses to large glycoprotein, fusion protein, nucleoprotein, phosphoprotein, and matrix protein were demonstrated in colostrum and milk. the iga antibody response was mainly directed against fusion pr ... | 1989 | 2778061 |
| evidence that the fusion protein of respiratory syncytial virus exists as a dimer in its native form. brief report. | the quaternary structure of respiratory syncytial virus (rsv) fusion protein has been studied. crosslinking studies were done to stabilize the noncovalently associated proteins. these stable, heat-resistant, covalently linked complexes were analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. in situ crosslinking studies demonstrated that the fusion protein of rsv exists as a dimer in its native form on the surface of infected cells. the purified protein was also found to be presen ... | 1989 | 2774976 |
| use of nasal and pharyngeal swabs for rapid detection of respiratory syncytial virus and adenovirus antigens by enzyme-linked immunosorbent assay. | nasal and pharyngeal swabs from 134 children with acute respiratory diseases were examined for the presence of respiratory syncytial (rs) virus and adenovirus antigens by enzyme-linked immunosorbent assay (elisa). the results were compared with those obtained by virus isolation and serology. altogether, 56 rs virus-positive (prospective study), 51 adenovirus-positive (retrospective study), and 27 negative (prospective study) samples were examined. the sensitivities of elisa were 96 and 98% for r ... | 1989 | 2768473 |
| the detection of bovine respiratory syncytial virus in formalin fixed bovine lung with commercially available monoclonal antibodies and avidin biotin complex immunohistochemistry. | eight commercially available monoclonal antibodies directed against respiratory syncytial virus antigens were tested for ability to detect bovine respiratory syncytial virus (brsv) antigen in formalin fixed, paraffin embedded bovine lung using avidin-biotin complex immunohistochemical staining. monoclonal antibodies from clone 18b2 purchased from biosoft, paris, france and those from clone 8g12 purchased from the department of veterinary sciences, university of nebraska, lincoln, nebraska staine ... | 1989 | 2766160 |
| the frequency, distribution and effects of antibodies, to seven putative respiratory pathogens, on respiratory disease and weight gain in feedlot calves in ontario. | during 1983-85, 279 calves requiring treatment for bovine respiratory disease and 290 comparison (control) animals from 15 different groups of feedlot calves were bled on arrival and again at 28 days postarrival. their sera were then analyzed for antibodies to seven putative respiratory pathogens. on arrival, the prevalences of indirect agglutination titers to pasteurella haemolytica, p. haemolytica cytotoxin, mycoplasma bovis and m. dispar were greater than 50%, the prevalence of titers to bovi ... | 1989 | 2766158 |
| prevalence of respiratory syncytial virus subgroups over six consecutive outbreaks: 1981-1987. | indirect immunofluorescence with strain-specific monoclonal antibodies was used to determine the phenotype of respiratory syncytial virus (rsv) isolates obtained from infants hospitalized in greater boston over six successive outbreaks from 1981 to 1987. of 981 isolates, 591 (60%) were classified as subgroup a and 383 (39%) as subgroup b. the prevalence of subgroups varied both between and within yearly outbreaks. in 1983-84 and 1984-85, both subgroups circulated concurrently and in almost equal ... | 1989 | 2760479 |
| bronchial reactivity in infants in acute respiratory failure with viral bronchiolitis. | airway reactivity and the effects of bronchodilators in infants are controversial. we studied the response to bronchodilator treatment in 14 mechanically ventilated infants (mean age, 2.74 months; range, 0.6-5.9) in respiratory failure caused by respiratory syncytial virus (rsv)-associated bronchiolitis. sixteen infants without lung disease, undergoing elective surgery, provided normal values. maximum expiratory deflation flow-volume (dfv) curves were produced by manual inflation of the lungs wi ... | 1989 | 2748221 |
| polypeptides of pneumonia virus of mice. ii. characterization of the glycoproteins. | the kinetics of synthesis and the nature of the oligosaccharides of the glycoproteins of pneumonia virus of mice (pvm) were studied. tryptic peptide mapping showed that the two major glycosylated polypeptides g1 and g2 were different forms of the same protein. g2 was derived from g1 which in turn appeared to be derived from an unidentified precursor. the g1/g2 protein of pvm is probably a haemagglutinin since a monoclonal antibody directed against it has a high haemagglutination inhibition titre ... | 1989 | 2732720 |
| polypeptides of pneumonia virus of mice. i. immunological cross-reactions and post-translational modifications. | a murine polyclonal antiserum and monoclonal antibodies have been employed to identify pneumonia virus of mice (pvm) polypeptides in infected cells and to study post-translational modifications. immunoprecipitation experiments using a murine polyclonal antiserum and a monoclonal antibody directed against a 39k protein have established an antigenic relationship between two pvm proteins and the n and p proteins of human respiratory syncytial virus. although 20 virus-specific polypeptides have been ... | 1989 | 2732719 |
| analysis of the antibody response to bovine respiratory syncytial virus proteins in calves. | the antibody response in calves to natural infections with bovine respiratory syncytial virus (brsv) was analysed by radioimmunoprecipitation assays. antibodies to virus proteins of mr 200k (l), 87k (g), 46k (f1), 41k (n), 35k (p), 28k and 24k (f2), 27k (m), 22k and less than 14k could be identified. recovery of 6- to 7-month-old calves from severe brsv-associated disease was accompanied by the development of an antibody response to the virus, which was directed mainly o the f and n proteins. ca ... | 1989 | 2732702 |
| immune response to experimentally induced infection with respiratory syncytial virus: possible role in the development of pulmonary disease. | groups of mice were immunized intraperitoneally with hep-2 lysate or respiratory syncytial virus (rsv)-infected hep-2 cells with or without adjuvant(s). the animals were subsequently challenged intranasally (i.n.) with rsv and/or hep-2 lysate and studied for rsv shedding in the lung, antigen-specific antibody response and pulmonary histopathology. a significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups t ... | 1989 | 2732693 |
| in vitro enhancement of respiratory syncytial virus infection of u937 cells by human sera. | human sera containing respiratory syncytial (rs) virus-specific antibodies enhance rs virus infection of the u937 macrophage cell line. there was an increase in the number of cells expressing virus antigen when u937 cells were infected with rs virus in the presence of human serum compared to cells infected in the absence of human serum. human sera enhanced virus yield, as measured by the cell-released infectious virus, by an average of 50-fold compared to virus infection in the absence of human ... | 1989 | 2732688 |
| fatty acid acylation of the fusion glycoprotein of human respiratory syncytial virus. | we describe the covalent attachment of palmitate to the fusion glycoprotein of respiratory syncytial virus and the identification of the attachment site. labeling of respiratory syncytial virus-infected vero cells with [3h]palmitate, followed by the purification and subsequent analysis of the fusion glycoprotein in conjunction with polyacrylamide gel electrophoresis, demonstrated that the fatty acid is covalently attached to the f1 subunit of the fusion glycoprotein. the bound palmitate was sens ... | 1989 | 2732224 |
| pathogenesis of naturally acquired bovine respiratory syncytial virus infection in calves: morphologic and serologic findings. | lesions in 32 calves that died or were euthanatized during the course of severe natural infection with bovine respiratory syncytial virus (brsv) are described. all calves had been dyspneic for 1 to 2 days. at necropsy, lesions that could be related to dyspnea included congested and cyanotic mucosae and widespread petechiae. the lungs had various lesions in the cranioventral (cv) and caudodorsal (cd) portions. the cv portion of the lungs was consolidated, firm, and edematous. histologically, the ... | 1989 | 2729714 |
| the tucson children's respiratory study. ii. lower respiratory tract illness in the first year of life. | lower respiratory tract illnesses occurring during the first year of life in 1,179 healthy infants enrolled in the children's respiratory study, tucson, arizona, are described. the children, who use the pediatricians of a health maintenance organization, were enrolled into the study between may 1980 and january 1984. data were collected on signs, symptoms, and diagnosis for each illness; nasopharyngeal and throat swabs were collected at the acute visit for viral, chlamydial, and mycoplasmal cult ... | 1989 | 2729259 |
| mice immunized with recombinant vaccinia virus expressing dengue 4 virus structural proteins with or without nonstructural protein ns1 are protected against fatal dengue virus encephalitis. | we have constructed vaccinia virus recombinants expressing dengue virus proteins from cloned dna for use in experimental immunoprophylaxis. a recombinant virus containing a 4.0-kilobase dna sequence that codes for three structural proteins, capsid (c), premembrane (pre-m), and envelope (e), and for nonstructural proteins ns1 and ns2a produced authentic pre-m, e, and ns1 in infected cv-1 cells. mice immunized with this recombinant were protected against an intracerebral injection of 100 50% letha ... | 1989 | 2724416 |
| immunoglobulin-class-specific immune response to respiratory syncytial virus structural proteins in infants, children, and adults. | the protein specificities of igg, igm, and iga antibodies induced during respiratory syncytial virus (rsv) infection in 74 patients (4 weeks to 81 years of age) were investigated using the technique of immunoblotting. although the pattern of antibody reactivity varied among patients, most of the humoral immune response in all age groups was directed against the 48, 42, 35, and 27 k proteins. an infant's own antibody response was discernible in 55 of the 57 children below 1 year of age, despite t ... | 1989 | 2723614 |
| rna fingerprinting of respiratory syncytial virus using ribonuclease protection. application to molecular epidemiology. | we have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup a respiratory syncytial (rs) virus. rnas extracted from hep-2 cells infected with strains to be analyzed were hybridized with a 32p-labeled rna probe corresponding to the rs virus g glycoprotein (a2 strain). areas of nonhomology were detected by cleavage with ribonuclease a. using this technique, multiple distinct rna cleavage patterns could be distinguished among viral isolat ... | 1989 | 2723064 |
| interprotein disulfide bonding between f and g glycoproteins of human respiratory syncytial virus. | the envelope glycoprotein g, of human respiratory virus was purified by immunoaffinity chromatography using a monoclonal antibody reacting with g glycoprotein. the purified material was analyzed for its protein patterns and by western blot for its reactivity with specific monoclonal antibodies. in addition to the g specific proteins at 90 and 55 kilodalton (kda) range, high molecular weight species were coeluted with g protein. three high molecular weight species were noticed: one (140 kda) reac ... | 1989 | 2719556 |
| experimental polyvalent iscoms subunit vaccine induces antibodies that neutralize human and bovine respiratory syncytial virus. | the purpose of the present study was to evaluate experimentally, in guinea-pigs, the immunogenicity of respiratory syncytial (rs) virus subunit vaccines. immunostimulating complexes (iscoms), made from the surface proteins of both human (long) and bovine (a-51908) rs strains adsorbed to the adjuvant quil a, were assayed for their capacity to induce neutralizing antibodies, in comparison to experimental live virus vaccines. serums from animals vaccinated with either the human or bovine rs subunit ... | 1989 | 2718604 |
| detection of ige antibodies to bovine respiratory syncytial virus. | the role of ige antibodies against respiratory syncytial virus has attracted attention for both human and bovine disease. to detect such antibodies, we have developed an enzyme-linked immunosorbent assay (elisa) specific for bovine respiratory syncytial virus (brsv). firstly, antiserum strongly positive for brsv-specific ige was produced by immunizing a levamisole-treated calf with brsv. the presence and specificity of brsv-specific ige in this animal was confirmed with the praunitz-kustner (pk) ... | 1989 | 2718372 |
| characterization of prolonged apneic episodes associated with respiratory syncytial virus infection. | we used high-speed polygraphic recordings to document in detail the sequence of events during prolonged apneic spells in two infants with respiratory syncytial viral (rsv) infection. also, we elicited upper airway reflexes by stimulating the airway with saline during sleep. spontaneous prolonged apneic spells were recorded in both infants, and these consisted of two distinctly different apnea types. a number of similarities between apnea of prematurity, upper airway reflex apnea and the apneas i ... | 1989 | 2717245 |
| serum immunoglobulin g antibody subclass response to respiratory syncytial virus f and g glycoproteins after first, second, and third infections. | serum samples from 31 children who experienced two or three infections with respiratory syncytial virus (rsv) in the first four years of life were tested in an enzyme-linked immunosorbent assay to examine the immunoglobulin g (igg) subclass responses to the rsv f and g surface glycoproteins associated with primary infection and reinfection. we sought to determine whether the greater degree of glycosylation of the g glycoprotein was reflected in an igg subclass immune response more like that to a ... | 1989 | 2715331 |
| ribavirin in respiratory syncytial virus infection. | 1989 | 2705808 | |
| nosocomial viral respiratory infections. | nosocomial infections with respiratory tract viruses, particularly influenza and respiratory syncytial viruses, account for the majority of serious nosocomial viral disease. chronically ill, immunocompromised, elderly, and very young hosts are especially vulnerable to potentially life-threatening involvement of the lower respiratory tract. effective preventive strategies are based upon early accurate viral diagnosis and an appreciation of the epidemiology and mechanisms of transmission for each ... | 1989 | 2697050 |
| rapid detection of respiratory syncytial virus by a biotin-enhanced immunoassay: test performance by laboratory technologists and housestaff. | a biotin-enhanced enzyme immunoassay (eia) for respiratory syncytial virus (rsv) antigen detection (testpack rsv) was prospectively compared with virus isolation in cell culture and immunofluorescence. of 156 nasopharyngeal swab specimens from infants with respiratory symptoms, 81 (52%) yielded rsv in culture. compared with culture the sensitivity of the eia was 95% and specificity was 92%; the specificity increased to 97% with a blocking assay. compared with immunofluorescence the sensitivity o ... | 1989 | 2696927 |
| the immunobiology of respiratory syncytial virus: prospects for a vaccine. | 1989 | 2694814 | |
| antiviral factors in human milk: implications in respiratory syncytial virus infection. | 1989 | 2692385 | |
| immunization of cotton rats with the fusion (f) and large (g) glycoproteins of respiratory syncytial virus (rsv) protects against rsv challenge without potentiating rsv disease. | a formalin-inactivated respiratory syncytial virus (rsv) vaccine tested 22 years ago failed to protect infant vaccinees against rsv infection or disease. instead, lower respiratory tract disease was enhanced during subsequent infection by rsv. enhancement of pulmonary pathology is also observed when cotton rats are immunized with formalin-inactivated rsv and subsequently infected with this virus. a major question that must be addressed for each new paramyxovirus vaccine is whether the immunogen ... | 1989 | 2692334 |
| [outbreak of respiratory syncytial virus in a neonatal unit]. | described is an outbreak of nosocomial rsv infection, following the admission of a 20-day-old and 11-day-old newborn with lower respiratory infections, diagnosed as being caused by rsv through detection of viral antigens by direct fat (fluorescent antibody test) of nasopharyngeal samples. later on, 5 of the other 20 newborn in patients in the same care unit also showed positive results in direct fat, for rsv. the clinical manifestations of these patients were mild even in those cases with underl ... | 1989 | 2679277 |
| comparison of nasal brush and nasopharyngeal aspirate techniques in obtaining specimens for detection of respiratory syncytial viral antigen by immunofluorescence. | diagnosis of respiratory syncytial virus by antigen detection is dependent on obtaining adequate respiratory epithelial cells. two specimen collection methods, nasopharyngeal aspiration (npa) and nasal brushing (nb), were compared. thirty-two pediatric patients with presumed viral pneumonia or bronchiolitis (34 episodes) had both npa and nb performed. of 34 specimens 15 were culture-positive for respiratory syncytial virus. of these 12 npa samples and 10 nb samples had viral inclusions by immuno ... | 1989 | 2677955 |
| respiratory syncytial virus-induced acute lung injury in adult patients with bone marrow transplants: a clinical approach and review of the literature. | acute lung injury induced by respiratory syncytial virus (rsv) is a major cause of morbidity and mortality in patients who have undergone bone marrow transplantation. twenty-nine of the 74 patients who received bone marrow transplants at the university of minnesota during a 1-year period developed evidence of acute lung injury, and rsv was identified as the cause in 8. we discuss the clinical course of these 8 patients and offer a clinical approach to rsv infection occurring after bone marrow tr ... | 1989 | 2677595 |
| structure and cell surface maturation of the attachment glycoprotein of human respiratory syncytial virus in a cell line deficient in o glycosylation. | the synthesis of the extensively o-glycosylated attachment protein, g, of human respiratory syncytial virus and its expression on the cell surface were examined in a mutant chinese hamster ovary (cho) cell line, ldld, which has a defect in protein o glycosylation. these cells, used in conjunction with an inhibitor of n-linked oligosaccharide synthesis, can be used to establish conditions in which no carbohydrate addition occurs or in which either n-linked or o-linked carbohydrate addition occurs ... | 1989 | 2677404 |
| protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric fg glycoprotein. | the cotton rat model of experimental human respiratory syncytial virus (rsv) infection was used to study the efficacy of fg, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. fg contained the extracellular regions of the f (fusion) and g (attachment) glycoproteins of rsv. vaccination with fg resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from rsv challenge against both serogroup a and b virus strains. b ... | 1989 | 2677234 |
| viral etiology in acute lower respiratory infections in children from a closed community. | the aim of this study was to determine the viral agents associated with acute lower respiratory infections (alri) in young children. during a 2-yr period, 204 nasopharyngeal aspirates (npa) from children under 4 yr of age living in an orphanage and exhibiting febrile alri were studied by both indirect immunofluorescence (iif) and isolation in four cell lines. npa cell smears as well as tissue culture cells exhibiting cytopathic effect (cpe) or hemadsorption were stained by iif for respiratory sy ... | 1989 | 2675703 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and elisa does not justify discontinuation of virus isolation. | direct fluorescent antibody assay (dfa) using monoclonal antibody and enzyme-linked immunosorbent assay (elisa) for rapid detection of respiratory syncytial virus (rsv) in nasopharyngeal secretions (nps) were compared with conventional virus isolation and identification procedures in cell cultures. when 134 nps were examined from infants and young children with acute respiratory tract infection, 42 (31%) were culture-positive for rsv and 31 of these were detected by the appearance of a typical c ... | 1989 | 2674634 |
| immunosuppression of the antibody response to respiratory syncytial virus (rsv) by pre-existing serum antibodies: partial prevention by topical infection of the respiratory tract with vaccinia virus-rsv recombinants. | immunization strategies to prevent respiratory syncytial virus (rsv) disease will involve immunization of infants less than 2 months of age who possess maternally derived rsv antibodies. vaccinia-rsv recombinant viruses are useful tools for defining parameters important in immunization against rsv and also are being considered as live virus vaccines for use in humans. previous studies demonstrated that passively acquired rsv antibodies can suppress the immune response and the protective efficacy ... | 1989 | 2671259 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by dna-rna hybridization. | we have developed an rna-cdna hybridization assay for the detection of respiratory syncytial virus (rsv) rna in nasopharyngeal samples. we chose to use as probe a cdna complementary to the nucleocapsid protein gene of rsv, integrated into the plasmid vector pbr322. the lower limit of sensitivity of the assay is 8.2 x 10(2) pfu of the long strain of rsv. in throat washes with added cell-free virus, the assay can detect 3.3 x 10(3) pfu of rsv. respiratory secretions were collected from a group of ... | 1989 | 2671029 |
| antigenic characterization of respiratory syncytial virus associated with acute respiratory infections in uruguayan children from 1985 to 1987. | the occurrence of subgroup a and b strains of respiratory syncytial virus (rsv) was studied during three epidemic years, 1985 to 1987, in uruguay. a set of monoclonal antibodies was selected according to their reactivity with local rsv isolates and used for the typing of rsv directly in nasopharyngeal cells by indirect immunofluorescence. of 77 specimens, 69 could be typed as belonging to subgroup a or b, 5 could not be typed with the restricted set of monoclonal antibodies employed, and 3 react ... | 1989 | 2671013 |
| routine viral culture for pediatric respiratory specimens submitted for direct immunofluorescence testing. | from 1986 to 1987, 69 (25%) of 274 specimens from children with lower respiratory tract syndromes were positive for respiratory syncytial virus antigen by direct immunofluorescence assay (dfa). comprehensive viral culture was performed on all 205 dfa-negative specimens, and 72 specimens yielded viruses; 5 specimens yielded multiple agents. thus, 52% of specimens yielded a specific virus, supporting the routine use of viral culture. isolates from the dfa-negative specimens included respiratory sy ... | 1989 | 2671012 |
| development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection. | with virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (rsv) was studied in peripheral blood lymphocytes (pbl) in groups of infants with acute rsv infection and in other control groups of subjects during a community outbreak of rsv infection. no rsv-specific cellular cytotoxicity was observed in cord blood lymphocytes and in other uninfected controls. the pbl of infants with acute rs ... | 1989 | 2666570 |
| evaluation of the abbott testpack rsv enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. | the abbott testpack rsv assay (abbott laboratories, north chicago, ill.), a rapid (20-min) enzyme immunoassay, was compared with culture and direct immunofluorescence (dfa) of nasopharyngeal cells for the detection of respiratory syncytial virus (rsv) in nasopharyngeal swab specimens. nasopharyngeal swab specimens, collected from 234 infants, were placed in viral transport medium. portions of specimen in transport medium were used for each test. of 234 specimens, 70 (30%) were culture positive, ... | 1989 | 2666434 |
| mechanism of lung injury in cotton rats immunized with formalin-inactivated respiratory syncytial virus. | respiratory syncytial virus (rsv) seronegative cotton rats were immunized intramuscularly at four and eight weeks of age with either formalin-inactivated, alum-precipitated rsv grown in hep-2 cell tissue cultures or virus-free hep-2 cell tissue cultures similarly prepared. sham-immunized animals served as controls. at 12 weeks of age, all animals were challenged with 6 x 10(5) plaque forming units of live rsv via the intranasal route. animals were killed at predetermined days and evaluated for r ... | 1989 | 2655343 |
| respiratory syncytial virus alters electrophysiologic properties in cotton rat airway epithelium. | the effect of respiratory syncytial virus (rsv) infection on the electrophysiologic properties of the airway epithelium was studied in tracheas obtained from cotton rats, after in vivo exposure to the virus. rsv infection was documented by tissue culture infectivity and immunofluorescent antibody techniques. light microscopic studies of the tracheas 72 hours after exposure to rsv revealed normal epithelial morphology. rsv infection produced a 28% decrease in short-circuit current (isc) and an al ... | 1989 | 2654848 |
| use of high-speed rolling to detect respiratory syncytial virus in cell culture. | we examined the effect of motion on respiratory syncytial virus (rsv) growth in cell culture. infected cultures were incubated stationary, rolling, or on an orbital shaker. enzyme immunoassay (eia) results for cultures infected with high concentrations of a laboratory strain of rsv were similar for all incubation conditions. however, cultures infected with low concentrations of virus and rolled at 96 rpm had a significantly greater mean eia optical density (1.78 +/- 0.22) than cultures rolled at ... | 1989 | 2654185 |
| [atypical interstitial pneumonia (aip) in calves and young cattle in schleswig-holstein in conjunction with an infection by the bovine respiratory syncytial virus (brsv)]. | it is reported on atypical interstitial pneumonia (aip) in 16 mostly holstein-frisian calves and feeders from 13 different farms in schleswig-holstein in association with an infection by the respiratory syncytial virus (brsv). all animals were submitted with identical clinical histories. macroscopically the lung lesions were characterized by alveolar and interstitial edema and emphysema. microscopically there was a diffuse interstitial pneumonia with formation of hyaline membranes and multinucle ... | 1989 | 2653786 |
| [infection and asthma]. | infection is frequent in all stages and forms of asthma. it is a source of exacerbations of all degrees of severity. bacterial infection, especially with commensal organisms from the upper respiratory tract, may cause bronchial hyperreactivity (hrb) by various mechanisms, specific--microbial delayed hypersensitivity, probably rate; ige-dependent hypersensitivity others non-specific: bronchial inflammation, source of mediators of bronchial constriction; activation of complement, direct histamine ... | 1989 | 2653352 |
| comparison of the anti-respiratory syncytial virus activity and toxicity of papaverine hydrochloride and pyrazofurin in vitro and in vivo. | based on reports describing their broad antiviral activity, the toxicity and antiviral efficacy of papaverine hydrochloride and pyrazofurin against respiratory syncytial virus (rsv) infection were tested in vitro in tissue culture cells and in vivo in cotton rats. papaverine inhibited rsv replication in vitro; however, the median minimal toxic dose-median minimal inhibitory concentration ratios (mtd50:mic50) in vitro and in vivo for papaverine were less than 4. further work with this compound wa ... | 1989 | 2653219 |
| infections in pediatric orthotopic heart transplant recipients. | the infectious complications of 31 orthotopic heart transplants in 27 patients performed between 1982 and 1987 were reviewed. fifteen patients (56%) are alive 704 to 1829 days posttransplantation. five of the 27 patients died within the first week posttransplantation of noninfectious causes. infection occurred in 17 of the remaining 22 patients and was the major cause of death in 3 of the 12 fatalities. there were 10 proved and 4 probable bacterial infections. three of the 10 proved bacterial in ... | 1989 | 2649868 |
| the 1a protein of respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species. | the respiratory syncytial virus (rsv) 1a protein was previously identified as a 7.5-kilodalton (kda) nonglycosylated species that, on the basis of its predicted sequence determined from the sequence of its mrna, contains a hydrophobic central domain that was suggestive of membrane interaction. here, four major, structurally distinct intracellular species of the 1a protein were identified in cells infected by rsv or by a recombinant vaccinia virus expressing the 1a gene. the four species of 1a we ... | 1989 | 2649692 |
| infectious diseases of new-world camelids (nwc). | although there are notable infectious conditions that are capable of producing clinical disease in the nwc, overall, these species are quite healthy. of the bacterial diseases, enterotoxemia caused by clostridium perfringens types c and d would be deemed the most significant in north america, while type a also would be regarded as important in south america. other important bacterial infections of potential concern are tuberculosis, johne's disease, anthrax, malignant edema, actinomycosis, tetan ... | 1989 | 2647231 |
| respiratory virus infection in immunocompromised patients. | seventy-eight immunocompromised patients were prospectively evaluated for infection with respiratory viruses including parainfluenza viruses, respiratory syncytial virus, influenza viruses and adenoviruses beginning before marrow transplant and continuing to 60 days after transplant or discharge from hospital. patients were studied both on a fixed surveillance schedule and at any time upper or lower respiratory symptoms developed. fifteen (19%) patients had a respiratory virus detected including ... | 1989 | 2647186 |
| in situ enzyme immunoassay for antiviral susceptibility testing of respiratory syncytial virus. | an enzyme immunoassay (eia), performed directly on fixed infected monolayers of hep-2 cells in microtiter plates, was compared with the conventional plaque reduction assay (pra) method for the determination of antiviral activity of ribavirin against respiratory syncytial virus. a 50% reduction in virus replication was observed at 3.4 and 5.9 mg/l of the drug by eia and pra, respectively. eia is simple to perform and reproducible and has objective end points. moreover, eia has advantages over pra ... | 1989 | 2646911 |
| immunization of cotton rats with the human respiratory syncytial virus f glycoprotein produced using a baculovirus vector. | the f glycoprotein of respiratory syncytial virus in insect cells was produced using a baculovirus expression vector to examine its potential as a subunit vaccine. two different forms of the f glycoprotein were expressed: the intact f (f) glycoprotein and the truncated (ft) glycoprotein, in which the cooh-terminal anchor region was deleted. the f glycoprotein remained cell associated, whereas the ft glycoprotein was secreted into the media of infected cells. in contrast to the processing of the ... | 1989 | 2644369 |
| increases in plasma concentrations of a prostaglandin metabolite in acute airway obstruction. | plasma concentrations of a stable prostaglandin f2 alpha metabolite were measured by radioimmunoassay during and after recovery from acute airway obstruction in 15 infants. mean (sem) metabolite concentrations (ng/l) in plasma obtained both before (1033 (418)) and after (1470 (413)) initial treatment for airway obstruction were significantly higher than those obtained from the same subjects after resolution of the obstruction--25.5 (6.6)--and those obtained from two comparison groups. infants po ... | 1989 | 2641987 |