Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| listeria monocytogenes possesses adhesins for fibronectin. | listeria monocytogenes is a gram-positive, nonsporulating, food-borne pathogen of humans and animals that is able to invade many eukaryotic cells. several listerial surface components have been reported to interact with eukaryotic cell receptors, but the complete mechanism by which the bacteria interact with all of these cell types remains largely unknown. in this work, we found that l. monocytogenes binds to human fibronectin, a 450,000-da dimeric glycoprotein found in body fluids, on the surfa ... | 1999 | 10569795 |
| [a prospective study of meningitis diagnosed in a 3rd-level hospital during a 1-year period]. | one-year prospective observational study of meningitis diagnosed at a third level hospital. | 1999 | 10568149 |
| in vitro activity of levofloxacin against gram-positive bacteria. | the in vitro activity of levofloxacin was investigated against 256 clinical strains of four gram-positive genera (staphylococcus, streptococcus, enterococcus, and listeria). ofloxacin and ciprofloxacin were used as comparators. uniform susceptibility to levofloxacin was recorded among methicillin-susceptible staphylococci, streptococci other than streptococcus agalactiae, regardless of their being susceptible, intermediate, or resistant to penicillin (s. pneumoniae) or erythromycin (s. pyogenes ... | 1999 | 10567771 |
| cytosolic delivery of granzyme b by bacterial toxins: evidence that endosomal disruption, in addition to transmembrane pore formation, is an important function of perforin. | granule-mediated cell killing by cytotoxic lymphocytes requires the combined actions of a membranolytic protein, perforin, and granule-associated granzymes, but the mechanism by which they jointly kill cells is poorly understood. we have tested a series of membrane-disruptive agents including bacterial pore-forming toxins and hemolytic complement for their ability to replace perforin in facilitating granzyme b-mediated cell death. as with perforin, low concentrations of streptolysin o and pneumo ... | 1999 | 10567584 |
| prfa, the transcriptional activator of virulence genes, is upregulated during interaction of listeria monocytogenes with mammalian cells and in eukaryotic cell extracts. | most virulence genes of listeria monocytogenes are activated by the transcriptional regulator prfa. previous studies have shown that environmental parameters, such as temperature, ph, stress conditions and medium composition, affect the expression of prfa and prfa-dependent proteins. in this report, we demonstrate a threefold increase in prfa protein synthesis during infection of mammalian cells, which correlates with the increased activity of the plca promoter, the major prfa promoter. increase ... | 1999 | 10564496 |
| host cell signal transduction during listeria monocytogenes infection. | the facultative intracellular bacterial pathogen listeria monocytogenes invades and multiplies in many mammalian cell types. during the interaction with its host cells it strongly interferes with and modulates host cell functions. in the present review we summarize the current knowledge on the modulation of signal transduction pathways by secreted listerial products prior to bacterium-cell contact, during uptake, or while l. monocytogenes resides in the different intracellular compartments. | 1999 | 10562430 |
| autotrophic assimilation of co2 and c1-compounds by pathogenic bacteria. | we demonstrate for the first time that the pathogenic bacteria yersinia pseudotuberculosis and listeria monocytogenes (pathogens of saprozoonoses) are capable of chemolithoautotrophic assimilation of co2. low temperature is favorable for better absorption of co2 by these bacteria; this is supported by increased enzymatic activity of carbonic anhydrase acting as the supplier of the substrate to the site of carboxylation. data of radioisotopic methods indicate that assimilated labeled carbon of co ... | 1999 | 10561561 |
| [study of mechanism of the pathogenic bacteria adaptation to the environmental factors]. | 1999 | 10560060 | |
| rocket-based motility: a universal mechanism? | 1999 | 10559888 | |
| spatial control of actin polymerization during neutrophil chemotaxis. | neutrophils respond to chemotactic stimuli by increasing the nucleation and polymerization of actin filaments, but the location and regulation of these processes are not well understood. here, using a permeabilized-cell assay, we show that chemotactic stimuli cause neutrophils to organize many discrete sites of actin polymerization, the distribution of which is biased by external chemotactic gradients. furthermore, the arp2/3 complex, which can nucleate actin polymerization, dynamically redistri ... | 1999 | 10559877 |
| actin and phosphoinositide binding by the acta protein of the bacterial pathogen listeria monocytogenes. | the surface protein acta of the pathogenic bacterium listeria monocytogenes induces actin-driven movement of bacteria in the cytoplasm of infected host cells and serves as a model for actin-based motility in general. we generated and purified soluble recombinant fragments of acta and assessed their ability to interact with the acidic phospholipids phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, both implicated in the regulation of actin polymerization. purifie ... | 1999 | 10559250 |
| emerging technologies and their implications: where policy, science, and law intersect. | 1998 | 10557577 | |
| intraperitoneal immunity and pneumoperitoneum. | carbon dioxide (co(2)) pneumoperitoneum has been implicated as a possible factor in depressed intraperitoneal immunity. using in vitro functional assays, co(2) has been shown to decrease the function of peritoneal macrophages harvested from insufflated mice. however, an effective in vivo assessment is lacking. listeria monocytogenes (lm), an intracellular pathogen, has served as a well-established in vivo model to study cell-mediated immune responses in mice. this study examines the immune compe ... | 1999 | 10556454 |
| actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling. | studies of the actin-based motility of the intracellular pathogens listeria monocytogenes and shigella flexneri have provided important insight into the events occurring at the leading edges of motile cells. like the bacteria listeria and shigella, vaccinia virus, a relative of the causative agent of smallpox, uses actin-based motility to spread between cells. in contrast to listeria or shigella, the actin-based motility of vaccinia is dependent on an unknown phosphotyrosine protein, but the und ... | 1999 | 10553910 |
| activation of t cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. | we have previously reported that cd4+ t cells recognizing a peptide comprising residues 234-252 of the heat shock protein (hsp)70 of mycobacterium tuberculosis (m.tb) in the context of rt1.b mhc class ii molecule emerged in the peritoneal cavity during the course of listeria monocytogenes infection in rats and suppressed the inflammatory responses against listerial infection via il-10 production. we report in this work that pretreatment with peptide 234-252 of hsp70 derived from m.tb suppressed ... | 1999 | 10553084 |
| affinity mass spectrometry-based approaches for the analysis of protein-protein interaction and complex mixtures of peptide-ligands. | combined applications of affinity purification procedures and mass-spectrometric analyses (affinity mass spectrometry or affinity-directed mass spectrometry) have gained broad interest in various fields of biological sciences. we have extended these techniques to the purification and analysis of closely related peptides from complex mixtures and to the characterization of binding motifs and relative affinities in protein-protein interactions. the posttranslational modifications in the carboxy-te ... | 1999 | 10552900 |
| regulation of actin polymerization by arp2/3 complex and wasp/scar proteins. | 1999 | 10551802 | |
| increased expression of a plant actin gene during a biotrophic interaction between round-leaved mallow, malva pusilla, and colletotrichum gloeosporioides f. sp. malvae. | two actin genes, acta from the hemibiotrophic anthracnose fungus, colletotrichum gloeosporioides (penz.) penz. & sacc. f. sp. malvae, and act1 from its host, malva pusilla (sm.) were cloned from a cdna library developed from infected host tissue. the actin gene, acta, of c. gloeosporioides f. sp. malvae, which is similar to that of other euascomycetes, appears to be expressed constitutively. the actin gene of m. pusilla is most similar to one of the actin genes of arabidopsis thaliana that is un ... | 1999 | 10550630 |
| unusual food-borne pathogens. listeria monocytogenes, aeromonas, plesiomonas, and edwardsiella species. | although these four groups of organisms are perceived as infrequent food-borne pathogens or of dubious significance, increasing epidemiologic data indicate that l. monocytogenes is an emerging cause of infections, particularly gastroenteritis. furthermore, if data are ever generated that prove that most fecal isolates of aeromonas are involved in bacterial diarrhea, then aeromonads will become recognized as important food-borne pathogens. for plesiomonas and edwardsiella, recognition of possible ... | 1999 | 10549426 |
| antimicrobial activities of several parts of pinus brutia, juniperus oxycedrus, abies cilicia, cedrus libani and pinus nigra. | in this study, the antimicrobial activities of several parts of various trees grown in the kahramanmaraş region of turkey were investigated by the disc diffusion method. chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of pinus brutia ten., juniperus oxycedrus l., abies cilicia ant. & kotschy carr., cedrus libani a. rich. and pinus nigra arn. were prepared and tested against bacillus megaterium dsm 32, bacillus subtilis img 22, bacillus cereus fmc 19, escheric ... | 1999 | 10548751 |
| the listeria monocytogenes dnak chaperone is required for stress tolerance and efficient phagocytosis with macrophages. | listeria monocytogenes is a facultative intracellular pathogen which can escape bactericidal mechanisms and grow within macrophages. the intracellular environment of macrophages is one of the most stressful environments encountered by an invading bacterium during the course of infection. to study the role of the major stress protein, dnak, of l. monocytogenes in survival under intracellular stress induced by macrophage-phagocytosis as well as under extracellular environmental stresses, we cloned ... | 1999 | 10547061 |
| in vitro survival of listeria monocytogenes in human amniotic fluid. | a listeria monocytogenes strain (ca01/93), haemolytic and virulent in mice, was inoculated in 16 samples of sterile human amniotic fluid to study the growth curves and to provide more information about the pathophysiology of the microorganism in pregnancy. a control test for every analysis was performed with an escherichia coli strain (cn 02/95). regardless of the amniotic fluid samples were taken at 34th-39th week of gestation, when it expresses maximum ability to inhibit growth of e. coli, the ... | 1999 | 10546327 |
| recovery in embryonated eggs of viable but nonculturable campylobacter jejuni cells and maintenance of ability to adhere to hela cells after resuscitation. | the existence of a viable but nonculturable (vbnc) state has been described for campylobacter jejuni as it had been for a number pathogenic bacteria. three c. jejuni human isolates were suspended in surface water and subsequently entered the vbnc state. after starvation for 30 days, vbnc cells were inoculated in the yolk sacs of embryonated eggs. culturable cells were detected in a large proportion of the embryonated eggs inoculated with vbnc c. jejuni cells. recovered cells kept their adhesion ... | 1999 | 10543837 |
| bacterial adhesion at synthetic surfaces. | a systematic investigation into the effect of surface chemistry on bacterial adhesion was carried out. in particular, a number of physicochemical factors important in defining the surface at the molecular level were assessed for their effect on the adhesion of listeria monocytogenes, salmonella typhimurium, staphylococcus aureus, and escherichia coli. the primary experiments involved the grafting of groups varying in hydrophilicity, hydrophobicity, chain length, and chemical functionality onto g ... | 1999 | 10543814 |
| transposon-induced mutations in two loci of listeria monocytogenes serotype 1/2a result in phage resistance and lack of n-acetylglucosamine in the teichoic acid of the cell wall. | teichoic acid-associated n-acetylglucosamine and rhamnose have been shown to serve as phage receptors in listeria monocytogenes serotype 1/2a. we generated and characterized two single-copy tn916deltae mutants which were resistant to phage a118 and several other serotype 1/2a-specific phages. in one mutant the insertion was immediately upstream of the recently identified ptshi locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other ... | 1999 | 10543788 |
| infectious complications in chronic lymphocytic leukaemia. | infections are the major cause of morbidity and mortality in patients with chronic lymphocytic leukemia (cll). predisposition to infection in cll is mediated through various abnormalities including both the immune defects inherent in the primary disease (impairment in humoral and cellular immunity) and in the further immunosuppression related to the management of cll. hypogammaglobulinemia is probably the most important immune defect in terms of risk of severe bacterial infections, its frequency ... | 1999 | 10543369 |
| identification and disruption of lisrk, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in listeria monocytogenes. | lisrk encodes a two-component regulatory system in the food pathogen listeria monocytogenes lo28. following identification of the operon in an acid-tolerant tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol concentrations, and a significant reduction in virulence. | 1999 | 10542190 |
| reduced antilisterial activity of tnf-deficient bone marrow-derived macrophages is due to impaired superoxide production. | mice deficient for tnf ligand and receptor type 1 have demonstrated the importance of tnf in the host defense against listeria monocytogenes. to investigate the particular deficiency of macrophages derived from tnf/lymphotoxin (lt)-alpha(-/-) mice in antilisterial growth control, bone marrow-derived macrophages (bmdm) were used for in vitro infection experiments. after the combined treatment with ifn-gamma and lipopolysaccharide (lps), production of no by wild-type (wt) and tnf/lt-alpha(-/-) bmd ... | 1999 | 10540319 |
| the 213-amino-acid leucine-rich repeat region of the listeria monocytogenes inlb protein is sufficient for entry into mammalian cells, stimulation of pi 3-kinase and membrane ruffling. | the listeria monocytogenes inlb protein is a 630-amino-acid surface protein that mediates entry of the bacterium into a wide variety of cell types, including hepatocytes, fibroblasts and epithelial cells such as vero, hep-2 and hela cells. invasion stimulates host proteins tyrosine phosphorylation, pi 3-kinase activity and rearrangements in the actin cytoskeleton. we previously showed that inlb is sufficient for entry of inlb-coated latex beads into cells and recent results indicate that purifie ... | 1999 | 10540282 |
| regression of established b16f10 melanoma with a recombinant listeria monocytogenes vaccine. | we have previously shown that listeria monocytogenes, a gram-positive, facultative intracellular bacterium, is a potent vector for targeting tumor-specific antigens to the immune system. in the present study, we extend these studies to the highly tumorigenic mouse melanoma b16f10, transduced with a model tumor antigen. we are able to induce the regression of primary tumors and established lung metastases by parenteral immunization with a l. monocytogenes recombinant that expresses the same antig ... | 1999 | 10537307 |
| listeria endocarditis causing aortic root abscess and a fistula to the left atrium. | we report the case of a 74-year-old man who presented with endocarditis on a porcine aortic valve replacement. five of six blood cultures grew listeria monocytogenes. transoesophageal echocardiography demonstrated the presence of a cavity posterior to the aortic annulus, apparently communicating with the left atrium. the patient underwent successful aortic valve re-replacement. listeria endocarditis is rare with only 58 reported cases in the literature and is associated with high mortality. | 1999 | 10533212 |
| acquired antibiotic resistance in lactic acid bacteria from food. | acquired antibiotic resistance, i.e. resistance genes located on conjugative or mobilizable plasmids and transposons can be found in species living in habitats (e.g. human and animal intestines) which are regularly challenged with antibiotics. most data are available for enterococci and enteric lactobacilli. raw material from animals (milk and meat) which are inadvertantly contaminated with fecal matters during production will carry antibiotic resistant lactic acid bacteria into the final fermen ... | 1999 | 10532375 |
| isolation, characterization, cdna cloning, and antimicrobial properties of two distinct subfamilies of alpha-defensins from rhesus macaque leukocytes. | experiments to isolate and characterize rhesus macaque myeloid alpha-defensins (rmads) were conducted. seven rmad peptides were isolated and sequenced, and the cdnas encoding six of these peptides and one other alpha-defensin from bone marrow were also characterized. four of the rmads were found to be highly similar to human neutrophil alpha-defensins hnp-1 to hnp-3, while the remaining four peptides were much more similar to human enteric alpha-defensin hd-5. two alpha-defensin pairs differed o ... | 1999 | 10531277 |
| repeated administration of synthetic oligodeoxynucleotides expressing cpg motifs provides long-term protection against bacterial infection. | synthetic oligodeoxynucleotides (odn) expressing unmethylated cpg motifs stimulate an innate immune response characterized by the production of polyreactive immunoglobulin m antibodies and immunomodulatory cytokines. this immune response has been shown to protect mice from challenge by listeria monocytogenes and francisella tularensis for up to 2 weeks. by repeatedly administering cpg odn two to four times/month, we found that this protection could be maintained indefinitely. protection was asso ... | 1999 | 10531213 |
| role of actin-filament disassembly in lamellipodium protrusion in motile cells revealed using the drug jasplakinolide. | in motile cells, protrusion of the lamellipodium (a type of cell margin) requires assembly of actin monomers into actin filaments at the tip of the lamellipodium. the importance of actin-filament disassembly in this process is less well understood, and is assessed here using the actin drug jasplakinolide, which has two known activities - inhibition of filament disassembly and induction of an increase in actin polymer. | 1999 | 10531004 |
| combined action of nisin and carvacrol on bacillus cereus and listeria monocytogenes. | nisin, a small antimicrobial protein, was tested for its bactericidal action against listeria monocytogenes and bacillus cereus and a typical biphasic reduction of the viable count was observed. the reduction was most fast during the first 10 min of exposure, while the viable count remained stable in the last part of the exposure period. bacillus cereus was more sensitive towards nisin than l. monocytogenes and the inhibitory effect of nisin was stronger towards cells cultivated and exposed at 8 ... | 1999 | 10530038 |
| effects of iron and selenium on the production of catalase, superoxide dismutase, and listeriolysin o in listeria monocytogenes. | listeria monocytogenes 19112, scott a, and 10403s were grown in tryptic soy broth (tsb) and tsb supplemented with 25 to 100 microg/ml of iron (fe) and 0.5 to 2.5 microg/ml selenium (se) to examine the effects on catalase (ca), superoxide dismutase (sod), and listeriolysin o (llo) activities. growth in tsb supplemented with fe resulted in significant increases in ca, sod, and llo activities in all three strains when compared to growth in tsb. the addition of 0.5 microg/ml se to tsb resulted in si ... | 1999 | 10528728 |
| a predictive model to determine the effects of ph, milkfat, and temperature on thermal inactivation of listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen of significance because of its comparatively high heat resistance, zero tolerance in ready-to-eat foods, and growth at refrigeration temperatures. a 3 x 3 x 3 factorial study was done to determine the effects of milkfat (0%, 2.5%, 5.0%), ph (5.0, 6.0, 7.0), and processing temperature (55 degrees c, 60 degrees c, 65 degrees c) on the thermal resistance of l. monocytogenes in a formulated and homogenized milk system. data were fit to a modified gomper ... | 1999 | 10528717 |
| irradiation and modified atmosphere packaging for the control of listeria monocytogenes on turkey meat. | when radiation-sterilized ground turkey meat was inoculated with listeria monocytogenes, packaged under mixtures of nitrogen and carbon dioxide, and irradiated with gamma-radiation doses of 0 to 3.0 kgy, there was a statistically significant (p < 0.05), but probably not a biologically significant, lower (0.39 log) predicted bacterial survival in the presence of 100% carbon dioxide than in the presence of 100% nitrogen. possibly because all atmospheres contained oxygen and because a response surf ... | 1999 | 10528716 |
| behavior of listeria monocytogenes and aeromonas spp. on fresh-cut produce packaged under equilibrium-modified atmosphere. | storage experiments were conducted to follow the behavior of pathogens on fresh-cut vegetables (trimmed brussels sprouts, grated carrots, shredded iceberg lettuce, and shredded chicory endives) packaged under an equilibrium-modified atmosphere (ema) (2 to 3% o2, 2 to 3% co2, and 94 to 96% n2) and stored at 7 degrees c. as a comparison, fresh-cut vegetables were also packaged in a perforated high-barrier film (air conditions) and stored at 7 degrees c. in a first step, the shelf life of the veget ... | 1999 | 10528715 |
| reduction of listeria monocytogenes and escherichia coli o157:h7 numbers on vacuum-packaged fresh beef treated with nisin or nisin combined with edta. | nisin or nisin combined with edta was used to treat fresh beef. beef cubes (2.5 by 2.5 by 2.5 cm) that were inoculated with approximately 7 log cfu/ml of listeria monocytogenes scott a or escherichia coli o157:h7 505 b were dipped in the following solutions: (i) h2o, (ii) hcl, (iii) nisin, (iv) edta, or (v) nisin combined with edta, respectively, for 10 min each, with an exception of one set of control beef samples without treatment. beef samples were then drip-dried for 15 min, vacuum packaged, ... | 1999 | 10528714 |
| insertional inactivation of the listeria monocytogenes cheya operon abolishes response to oxygen gradients and reduces the number of flagella. | the nucleotide sequence of a region downstream of the listeria monocytogenes flagellin gene, flaa, revealed two putative chemotaxis genes, chey and chea. these genes have been shown to be transcribed as a bicistronic unit. in this study tn916 delta e mutagenesis was used to generate two mutants, pf10 and pf16, which contain transposon inserts in the promoter region of this operon. these mutants were motile in liquid, but had reduced flagellin expression and were unable to burrow or swarm on soft ... | 1999 | 10528396 |
| reconstitution of actin-based motility of listeria and shigella using pure proteins. | actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions. the arp2/3 complex is required to stimulate actin assembly at the leading edge in response to signalling. the bacteria listeria and shigella bypass the signalling pathway and harness the arp2/3 complex to induce actin assembly and to propel themselves in living cells. however, the arp2/3 complex alone is insufficient to promote movement. here we have used pure componen ... | 1999 | 10524632 |
| cell motility. bare bones of the cytoskeleton. | 1999 | 10524617 | |
| purification and amino acid sequence of lactocin 705, a bacteriocin produced by lactobacillus casei crl 705. | lactobacillus casei crl 705, isolated from a dry fermented sausage, produces an antibacterial peptide which is active against listeria monocytogenes. previous studies have shown that this compound is potentially useful to control food-borne pathogens in ground meat. in view of the potential application of this antimicrobial substance in food fermentation, a detailed biochemical analysis of this peptide is required. in this work, the purification and amino acid sequence of this bacteriocin is pre ... | 1999 | 10522386 |
| estimation of temperature dependent growth rate and lag time of listeria monocytogenes by optical density measurements. | an automated turbidimetric system, bioscreen c, was used to monitor growth of ten strains of listeria monocytogenes at different temperatures. several methods for estimation of maximum specific growth rate (mu(max)) and lag time (lag) from turbidimetric data were compared to values estimated from viable count data. by using a calibration factor, reliable estimations of mu(max) could be obtained from turbidimetric measurements. on the other hand, accurate estimations of lag required some viable c ... | 1999 | 10520594 |
| curcumin inhibits th1 cytokine profile in cd4+ t cells by suppressing interleukin-12 production in macrophages. | 1 interleukin-12 (il-12) plays a central role in the immune system by driving the immune response towards t helper 1 (th1) type responses which are characterized by high ifn-gamma and low il-4 production. in this study we investigated the effects of curcumin, a natural product of plants obtained from curcuma longa (turmeric), on il-12 production by mouse splenic macrophages and the subsequent ability of these cells to regulate cytokine production by cd4+ t cells. 2 pretreatment with curcumin sig ... | 1999 | 10510448 |
| effective dna vaccination against listeriosis by prime/boost inoculation with the gene gun. | protective immunity against listeria monocytogenes strongly depends on cd8+ t lymphocytes, and both ifn-gamma secretion and target cell killing are considered relevant to protection. we analyzed whether we could induce a protective type 1 immune response by dna vaccination with the gene gun using plasmids encoding for two immunodominant listerial ags, listeriolysin and p60. to induce a th1 response, we 1) coprecipitated a plasmid encoding for gm-csf, 2) employed a prime/boost vaccination schedul ... | 1999 | 10510394 |
| cutting edge: paradigm revisited: antibody provides resistance to listeria infection. | listeriolysin o (llo) is a secreted pore-forming toxin of the facultative intracellular bacterium listeria monocytogenes. we assessed the ability of a murine anti-llo mab to affect the course of infection in mice challenged with listeria. this mab was previously shown to be capable of neutralizing llo-mediated pore formation in vitro, and here we show that the passive administration of this ab to mice before infection provides increased resistance. mice treated with the mab were protected from a ... | 1999 | 10510340 |
| survival of listeria monocytogenes in commercial cheese brines. | the survival of listeria monocytogenes was determined in commercial cheese brines collected from cheese factories in wisconsin and northern illinois. survival of l. monocytogenes inoculated into commercial cheese brines ranged from < 7 d to over 259 d. survival did not correlate with ph, salt content, nitrogen content, mineral content, or inherent microbial populations but was negatively associated with addition of sodium hypochlorite at the dairy plant. the l. monocytogenes generally survived l ... | 1999 | 10509244 |
| detection and differentiation of listeria spp. by a single reaction based on multiplex pcr. | the iap gene encodes the protein p60, which is common to all listeria species. a previous comparison of the dna sequences indicated conserved and species-specific gene portions. based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation of listeria species with a single multiplex pcr and subsequent gel analysis was selected. one primer was derived from the conserved 3' end and is specific for all listeria species; th ... | 1999 | 10508109 |
| effect of milk proteins on adhesion of bacteria to stainless steel surfaces. | stainless steel coupons were treated with skim milk and subsequently challenged with individual bacterial suspensions of staphylococcus aureus, pseudomonas fragi, escherichia coli, listeria monocytogenes, and serratia marcescens. the numbers of attached bacteria were determined by direct epifluorescence microscopy and compared with the attachment levels on clean stainless steel with two different surface finishes. skim milk was found to reduce adhesion of s. aureus, l. monocytogenes, and s. marc ... | 1999 | 10508087 |
| [listeria monocytogenes pneumonia: a severe complication in a patient with neoplasia]. | listeriosis is a infectious disease which can affect to immunosuppressed patients. we report a case of pneumonia by listeria monocytogenes as unusual presentation, because the central nervous system infections are the most common clinical entities. many cases reported had a underlying disease, most commonly hodgkin's disease, and had received immunosuppressive therapy. in our case the patient was considered to be immunosuppressed by had received corticosteroids and had been diagnosed of stage ii ... | 1999 | 10507170 |
| the isolation of certain pathogen microorganisms from raw milk. | in this research, a total of 211 raw milk samples in different areas of ankara were collected from various dairy plants. each of these samples was tested for the presence of yersinia enterocolitica, listeria monocytogenes and campylobacter spp. from a total of 14 (6.63%) y. enterocolitica and y. enterocolitica-like organisms were isolated. 8 (3.79%) of the total isolation were identified as y. enterocolitica whereas 6 (2.84%) of these were found to be y. enterocolitica-like organisms. in additio ... | 1999 | 10499148 |
| aromatic and basic residues within the evh1 domain of vasp specify its interaction with proline-rich ligands. | short contiguous peptides harboring proline-rich motifs are frequently involved in protein-protein interactions, such as associations with src homology 3 (sh3) and ww domains. although patches of aromatic residues present in either domain interact with polyprolines, their overall structures are distinct, suggesting that additional protein families exist that use stacked aromatic amino acids (aa domains) to bind polyproline motifs [1] [2] [3]. a polyproline motif (e/dfpppptd/e in the single-lette ... | 1999 | 10498433 |
| ubiquicidin, a novel murine microbicidal protein present in the cytosolic fraction of macrophages. | previously we have identified and characterized three murine microbicidal proteins purified from the granule fraction of cells from the murine macrophage cell line raw264.7. during these studies evidence was obtained for the presence of an additional antimicrobial protein in the cytosolic fraction of raw264.7 cells that had been activated with interferon-gamma (ifn-gamma). in this study we have purified this protein, designated ubiquicidin, to apparent homogeneity and demonstrated that it is a c ... | 1999 | 10496312 |
| fifteen years of experience with bacterial meningitis. | introduction of haemophilus influenzae type b (hib) vaccines has dramatically altered the epidemiology of bacterial meningitis in children. the goal of this study was to describe these changes in a pediatric teaching hospital. | 1999 | 10493344 |
| antibiotics in neonatal infections: a review. | the bacteria most commonly responsible for early-onset (materno-fetal) infections in neonates are group b streptococci, enterococci, enterobacteriaceae and listeria monocytogenes. coagulase-negative staphylococci, particularly staphylococcus epidermidis, are the main pathogens in late-onset (nosocomial) infections, especially in high-risk patients such as those with very low birthweight, umbilical or central venous catheters or undergoing prolonged ventilation. the primary objective of the paedi ... | 1999 | 10493270 |
| mhc class i restricted t cell responses to listeria monocytogenes, an intracellular bacterial pathogen. | studies of the murine immune response to infection with the intracellular bacterial pathogen listeria monocytogenes have provided a wealth of information about innate and acquired immune defenses in the setting of an infectious disease. our studies have focused on the mhc class i restricted, cd8+ t cell responses of balb/c mice to l. monocytogenes infection. four peptides that derive from proteins that l. monocytogenes secretes into the cytosol of infected cells are presented to cytotoxic t lymp ... | 1999 | 10493175 |
| phenotypic modulation by intracellular bacterial pathogens. | microorganisms have the capacity to sense their environment and to respond to it by alteration in gene expression and protein synthesis. two-dimensional electrophoresis (2-de) provides a powerful tool to examine the global response in bacterial protein synthesis upon exposure to different environmental signals. one of the most complex environments encountered by facultative intracellular pathogenic bacteria is the intracellular environment of the host cell. numerous studies have documented that ... | 1999 | 10493129 |
| a proteomic approach to study the acid response in listeria monocytogenes. | the responses of listeria monocytogenes to acidic conditions were studied at the level of protein synthesis at a lethal acidic ph (acid stress) and an intermediary nonlethal acidic ph (acid adaptation). the radiolabeled acid-induced proteins were separated by two-dimensional (2-d) electrophoresis and analyzed by a computer-aided 2-d gel analysis system. the two acidic conditions upgraded a number of constitutive proteins and induced synthesis of a number of novel proteins. the majority of these ... | 1999 | 10493126 |
| development of a lacticin 3147-enriched whey powder with inhibitory activity against foodborne pathogens. | the broad-spectrum bacteriocin lacticin 3147, produced by lactococcus lactis dpc3147, is inhibitory to a wide range of gram-positive food spoilage and pathogenic organisms. a 10% solution of demineralized whey powder was fermented with dpc3147 at a constant ph of 6.5. the fermentate was spray dried, and the resulting powder exhibited inhibitory activity. the ability of the lacticin 3147-enriched powder to inhibit listeria monocytogenes scott a and staphylococcus aureus 10 was assessed in buffer ... | 1999 | 10492475 |
| nisin reduces the thermal resistance of listeria monocytogenes scott a in liquid whole egg. | d-values (decimal reduction times) and z-values (increase in temperature required for a 1-log change in d-value) for listeria monocytogenes scott a were determined in liquid whole egg with nisin (0 or 10 microg ml(-1)) and nacl (0 or 10%) by a submerged glass ampoule procedure. samples were plated onto nonselective agar at appropriate intervals, and d-values were determined using a modified biphasic logistic equation. addition of nacl increased d-values at all temperatures tested. the addition o ... | 1999 | 10492473 |
| effects of the growth procedure on the surface hydrophobicity of listeria monocytogenes cells and their adhesion to stainless steel. | the aim of this study was to examine the physicochemical surface properties and the ability to adhere to stainless steel of three strains of listeria monocytogenes after different cultivation procedures. to this end, bacteria were cultivated at 37 degrees c after storage at two frequently used temperatures (4 degrees c or -80 degrees c) and were then transferred into the liquid medium (trypticase soy broth supplemented with 6 g liter(-1) of yeast extract, ph 7.3) between one and four times. in a ... | 1999 | 10492472 |
| predictive thermal inactivation model for listeria monocytogenes with temperature, ph, nacl, and sodium pyrophosphate as controlling factors. | the effects and interactions of heating temperature (55 to 65 degrees c), ph (4 to 8), salt (nacl; 0 to 6%, wt/vol), and sodium pyrophosphate (spp; 0 to 0.3%, wt/vol) on the heat inactivation of a four-strain mixture of listeria monocytogenes in beef gravy were examined. a factorial experimental design comparing 48 combinations of heating temperature, salt concentration, ph value, and spp content was used. heating was carried out using a submerged-coil heating apparatus. the recovery medium was ... | 1999 | 10492471 |
| listeria monocytogenes exploits normal host cell processes to spread from cell to cell. | the bacterial pathogen, listeria monocytogenes, grows in the cytoplasm of host cells and spreads intercellularly using a form of actin-based motility mediated by the bacterial protein acta. tightly adherent monolayers of mdck cells that constitutively express gfp-actin were infected with l. monocytogenes, and intercellular spread of bacteria was observed by video microscopy. the probability of formation of membrane-bound protrusions containing bacteria decreased with host cell monolayer age and ... | 1999 | 10491395 |
| activation of the cdc42 effector n-wasp by the shigella flexneri icsa protein promotes actin nucleation by arp2/3 complex and bacterial actin-based motility. | to propel itself in infected cells, the pathogen shigella flexneri subverts the cdc42-controlled machinery responsible for actin assembly during filopodia formation. using a combination of bacterial motility assays in platelet extracts with escherichia coli expressing the shigella icsa protein and in vitro analysis of reconstituted systems from purified proteins, we show here that the bacterial protein icsa binds n-wasp and activates it in a cdc42-like fashion. dramatic stimulation of actin asse ... | 1999 | 10491394 |
| the development of a combined surface adhesion and polymerase chain reaction technique in the rapid detection of listeria monocytogenes in meat and poultry. | a procedure combining enrichment surface adhesion and polymerase chain detection (sa-pcr) was developed and applied in the detection of listeria monocytogenes in meat products. minced beef samples were inoculated with l. monocytogenes (log(10)3 cfu g(-1)) and incubated for 10 h at 30 degrees c in buffered peptone water. l. monocytogenes was recovered from the culture by attachment to a polycarbonate membrane immersed for 15 min in the enriched meat culture. the membrane and attached bacteria wer ... | 1999 | 10490225 |
| the relationship between acid stress responses and virulence in salmonella typhimurium and listeria monocytogenes. | all pathogenic bacteria possess the ability to evade or surmount body defenses (stresses, as experienced by the bacterium) long enough to cause a sufficient reaction, which is then manifested as a disease or illness. while opportunistic pathogens will only cause illness in the event of a predisposing weakness in these defenses, many pathogens must take on and overcome intact defenses. this is particularly true of gastrointestinal pathogens such as listeria monocytogenes and salmonella spp., whic ... | 1999 | 10488846 |
| listeria monocytogenes infection of caco-2 human epithelial cells induces activation of transcription factor nf-kappa b/rel-like dna binding activities. | the effect of listeria monocytogenes infection on the cellular level of transcription factor nf-kappa b in the human epithelia-like cell line caco-2 was investigated. infection with l. monocytogenes or treatment with lipoteichoic acid induced the formation of three nf-kappa b-like dna-protein complexes c1, c2, and c3, which were identified as containing either, rela and p50, relb and p50, or p50, respectively. nf-kappa b activation in l. monocytogenes-infected caco-2 cells was distinct from nf-k ... | 1999 | 10483730 |
| [bacteria killing by macrophages via nf-il6 gene dependent mechanism: the susceptibility to mycobacterium leprae in nf-il6 knockout mice]. | transcription factor, nf-il6 recognizes the same nucleotide sequences as c/ebp, and it is predominantly expressed in macrophages. tanaka et al. reported that nf-il6 knockout mice are highly susceptible to listeria monocytogenes and salmonella typhimurium due to impairment of bacteria killing by activated macrophages. we have tried to see the susceptibility for mycobacterium leprae infection with intraperitoneal(i.p.) or both hind foot pad (bhf) in the nf-il6 knockout mice with wild control mice. ... | 1999 | 10481452 |
| listeria monocytogenes and recurrent mycobacterial infections in a child with complete interferon-gamma-receptor (ifngammar1) deficiency: mutational analysis and evaluation of therapeutic options. | we describe the history of a girl with interferon-gamma-receptor (ifngammar1) deficiency and studies performed to identify the molecular and clinical characteristics of this recently discovered disorder. this is the first report of a child from northern europe with ifngammar1 deficiency. the patient, now 7 years old, first presented with swelling and reddening at the bacille calmette-guerin (bcg) vaccination site, swelling of lymph nodes, hepatomegaly, and an unusually severe varicella rash at t ... | 1999 | 10480427 |
| modulation of intracellular growth of listeria monocytogenes in human enterocyte caco-2 cells by interferon-gamma and interleukin-6: role of nitric oxide and cooperation with antibiotics. | the influence of interferon (ifn)-gamma and interleukin (il)-6 on the intracellular growth of listeria monocytogenes phagocytosed from the apical pole was examined in polarized caco-2 cells. ifn-gamma (from the apical pole) and il-6 (from the basolateral pole) considerably reduced the bacterial intracellular growth, an effect largely abolished by l-monomethyl arginine. both cytokines caused overexpression of inducible nitric oxide synthase. il-6, but not ifn-gamma, caused a partial restriction o ... | 1999 | 10479148 |
| suppression of acquired immunity against listeria monocytogenes by amphotericin b-mediated inhibition of cd8 t cell function. | amphotericin b is frequently used for the treatment of fungal infections of immunocompromised individuals. whereas immunomodulatory side effects of this agent are known, the influence of amphotericin b was studied in the model of murine listeria monocytogenes infection. treatment of l. monocytogenes-immune mice with a nontoxic dose of amphotericin b (0.75 mg/kg) reduced antilisterial protection by 4-5 orders of magnitude, while it had no significant effect on natural immunity against l. monocyto ... | 1999 | 10479147 |
| a monoclonal antibody directed against the murine macrophage surface molecule f4/80 modulates natural immune response to listeria monocytogenes. | whole spleen cell cultures from scid mice release high levels of ifn-gamma when exposed to heat-killed listeria monocytogenes (hkl). this microbe-induced and t cell-independent response depends on both macrophages (mphi) and nk cells: hkl-stimulated mphi release tnf-alpha and il-12, which together activate nk cells for ifn-gamma release. we show here that this cytokine-mediated activation cascade can be modulated by a mab against the mphi surface glycoprotein f4/80. hkl-induced il-12, tnf-alpha, ... | 1999 | 10477612 |
| genomic typing of listeria monocytogenes strains by automated laser fluorescence analysis of amplified fragment length polymorphism fingerprint patterns. | the genetic relationship between isolates of listeria monocytogenes belonging to different serotypes was determined and the suitability of automated laser fluorescent analysis (alfa) of amplified fragment length polymorphism (aflp) fingerprints was assessed by genomic typing of 106 l. monocytogenes isolates belonging to serotypes 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a, 4ab, 4b, 4c, 4d, 4e, 1, and 7. digitised aflp fingerprints were obtained that showed approximately 50 clearly distinguishable selectiv ... | 1999 | 10477075 |
| bacterial populations associated with a sorghum-based fermented weaning cereal. | microbiological surveys, to determine the quality and safety, were conducted on 45 sorghum samples comprising dry powders (n = 15) and corresponding fermented (n = 15) and cooked fermented porridge (n = 15) samples collected from households in an informal settlement of the gauteng province of south africa. mean aerobic plate counts, gram-negative counts and bacterial spore counts of sorghum powder samples decreased in fermented and cooked fermented porridge samples. however, mean lactic acid bac ... | 1999 | 10477073 |
| efficacy of electrolyzed oxidizing water for inactivating escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes. | the efficacy of electrolyzed oxidizing water for inactivating escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes was evaluated. a five-strain mixture of e. coli o157:h7, s. enteritidis, or l. monocytogenes of approximately 10(8) cfu/ml was inoculated in 9 ml of electrolyzed oxidizing water (treatment) or 9 ml of sterile, deionized water (control) and incubated at 4 or 23 degrees c for 0, 5, 10, and 15 min; at 35 degrees c for 0, 2, 4, and 6 min; or at 45 degrees c for 0 ... | 1999 | 10473453 |
| variation in resistance to hydrostatic pressure among strains of food-borne pathogens. | among food-borne pathogens, some strains could be resistant to hydrostatic pressure treatment. this information is necessary to establish processing parameters to ensure safety of pressure-pasteurized foods (n. kalchayanand, a. sikes, c. p. dunne, and b. ray, j. food prot. 61:425-431, 1998). we studied variation in pressure resistance among strains of listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, and salmonella species at two temperatures of pressurization. early-stati ... | 1999 | 10473446 |
| identification of an atp-driven, osmoregulated glycine betaine transport system in listeria monocytogenes. | the ability of the gram-positive, food-borne pathogen listeria monocytogenes to tolerate environments of elevated osmolarity and reduced temperature is due in part to the transport and accumulation of the osmolyte glycine betaine. previously we showed that glycine betaine transport was the result of na(+)-glycine betaine symport. in this report, we identify a second glycine betaine transporter from l. monocytogenes which is osmotically activated but does not require a high concentration of na(+) ... | 1999 | 10473414 |
| the antibacterial efficacy of trovafloxacin against an experimental infection with listeria monocytogenes in hydrocortisone-treated mice. | the efficacy of trovafloxacin in treating listeria monocytogenes infections in glucocorticosteroid-treated mice was compared with the efficacy of amoxycillin. swiss mice were treated with daily injections of 2.5 mg hydrocortisone s.c. and then infected i.v. with 1 x 10(7) cfu of l. monocytogenes. untreated, this level of infection resulted in 100% mortality between day 3 and day 5 after infection. both s.c. trovafloxacin and amoxycillin were effective in reducing the number of viable l. monocyto ... | 1999 | 10473230 |
| bacterial meningitis and the newborn infant. | bacterial meningitis in the neonate differs from meningitis in the older infant and child in a number of ways. bacterial pathogens primarily are associated with the maternal genitourinary tract. symptoms and physical findings may be nonspecific, and a high index of suspicion is needed. management may vary depending on the maturity of the infant and the bacterial pathogen that is isolated. | 1999 | 10470563 |
| the role of kupffer cells and regulation of neutrophil migration into the liver by macrophage inflammatory protein-2 in primary listeriosis in mice. | depletion of mouse kupffer cells and splenic macrophages following intravenous administration of liposome-entrapped clodronate severely reduced host resistance to primary infection with listeria monocytogenes. infection of clodronate-treated mice with a sublethal dose of l. monocytogenes resulted in death of the mice within 3 days. the macrophage depletion resulted in marked increases in bacterial growth in the liver and spleen, but not in other tissues. the proliferation of l. monocytogenes was ... | 1999 | 10469395 |
| sulfasalazine prevents t-helper 1 immune response by suppressing interleukin-12 production in macrophages. | interleukin-12 (il-12) plays a pivotal role in the development of t-helper 1 (th1) immune response, which may be involved in the pathogenesis of chronic inflammatory autoimmune disorders. in this study we investigated the effects of sulfasalazine, a drug for treating inflammatory bowel disease and rheumatoid arthritis, on the production of il-12 from mouse macrophages stimulated with lipopolysaccharide (lps). sulfasalazine potently inhibited the production of il-12 in a dose-dependent manner, in ... | 1999 | 10469239 |
| the effects of chlorella vulgaris in the protection of mice infected with listeria monocytogenes. role of natural killer cells. | in this work we have demonstrated the effects of oral administration of chlorella vulgaris (cv) on natural killer cells (nk) activity of mice infected with a sublethal dose of viable listeria monocytogenes. the treatment with c. vulgaris produced a significant increase on nk cells activity in normal (non-infected) animals compared to the animals that received only vehicle (water) (p < 0.0001). similarly, the infection alone produced a significant increase on nk cells activity, which was observed ... | 1999 | 10466083 |
| isolation and identification of antimicrobial furocoumarins from parsley. | photoactive furocoumarins extracted from four varieties of fresh and freeze-dried parsley leaves inhibited a dna repair-deficient escherichia coli in a photobiological assay. using media-modified assays, the human pathogens e. coli o157:h7 and listeria monocytogenes, the spoilage microorganism erwinia carotovora, and listeria innocua were also inhibited. pseudomonas fragi was not inhibited. minimum concentrations of forest green parsley powder in agar which showed inhibition ranged from 0.12% to ... | 1997 | 10465045 |
| antimicrobial activity of sulfur compounds derived from cabbage. | selected sulfur compounds found in cabbage and its fermentation product, sauerkraut, were tested for minimum inhibitory concentration (mic) against growth of 15 species of bacteria and 4 species of yeasts. s-methyl-l-cysteine sulfoxide, sinigrin, and dimethyl sulfide at 500 ppm were not inhibitory to any of the bacteria and yeasts tested. dimethyl disulfide at 500 ppm retarded some, but did not prevent growth of any of the test microorganisms. dimethyl trisulfide had an mic to bacteria of 200 pp ... | 1997 | 10465044 |
| bactericidal effect of enterocin 4 on listeria monocytogenes in a model dairy system. | enterococcus faecalis inia 4 produced the bacteriocin enterocin 4 during growth in raw ewe's milk at 30 degrees c. enterocin activity reached 2,200 to 3,600 au/ml after 8 h, with a 1 to 8% (vol/vol) level of inoculum from an 18-h culture. an enterocin activity of 500 au/ml significantly decreased counts of listeria monocytogenes ohio when incubated for 6 h in a model system consisting of filtrates from cultures of e. faecalis inia 4 in raw ewe's milk, at ph 6.0 and 30 degrees c. however, an ente ... | 1997 | 10465036 |
| combined effects of packaging atmosphere and lactic acid on growth and survival of listeria monocytogenes in crayfish tail meat 4 degrees c. | the effect of lactic acid on growth and survival of listeria monocytogenes in crayfish tail meat stored under refrigeration and various gas environments was investigated. frozen crayfish tail meat was thawed overnight, autoclaved, cooled, and inoculated with approximately 4 log colony-forming units (cfu) of a mixed-strain (scott a and f5027) l. monocytogenes culture per gram of meat. inoculated samples were blended with 0, 0.5, 1.0, 1.5, or 2.0% lactic acid and packaged under air, vacuum, or mod ... | 1996 | 10463442 |
| monolaurin and acetic acid inactivation of listeria monocytogenes attached to stainless steel. | individual and combined antimicrobial effects of monolaurin and acetic acid on listeria monocytogenes planktonic cells or stainless-steel-adherent cells were determined in order to evaluate cell viability during a 25-min exposure period at 25 degrees c. a 10(7)-colony-forming units (cfu)/ml population of planktonic cells was completely inactivated by the synergistic combination of 1% acetic acid with 50 or 100 microg/ml of monolaurin within 25 or 20 min, respectively. either compound alone cause ... | 1996 | 10463441 |
| leishmania species: models of intracellular parasitism. | leishmania species are obligate intracellular parasites of cells of the macrophage-dendritic cell lineage. indeed, the ability to survive and multiply within macrophages is a feature of a surprising number of infectious agents of major importance to public health, including mycobacterium tuberculosis, mycobacterium leprae, listeria monocytogenes, salmonella typhimurium, toxoplasma gondii and trypanosoma cruzi. the relationship between such organisms and their host cells is particularly intriguin ... | 1999 | 10462516 |
| health risks associated with unpasteurized goats' and ewes' milk on retail sale in england and wales. a phls dairy products working group study. | a pilot study to determine the microbiological quality of unpasteurized milk from goats and ewes sampled from farm shops, health food shops, and other retail premises found that 47%, (47/100) of goats' and 50% (13/26) of ewes' milk samples failed the standards prescribed by the dairy products (hygiene) regulations 1995. in addition, staphylococcus aureus, haemolytic streptococci or enterococci, were present in excess of 10(2) c.f.u./ml in 9 (7 %) 2 (2 %) and 19 (15%) samples, respectively. salmo ... | 1999 | 10459642 |
| effects of chlorella vulgaris on bone marrow progenitor cells of mice infected with listeria monocytogenes. | in this study we investigated the effects of the treatment with chlorella vulgaris extract (cve) on the hematopoietic response of granulocyte-macrophage colony-forming unit (cfu-gm) of mice infected with a sublethal dose of listeria monocytogenes (1 x 10(4) organisms/animal). cve was given orally as 50 mg/kg/day for 5 days. in the cve treated/infected groups l. monocytogenes was administered at the end of cve treatment. the colony stimulating activity of the serum (csa) was also studied in all g ... | 1999 | 10458539 |
| beta-estradiol-induced decrease in il-12 and tnf-alpha expression suppresses macrophage functions in the course of listeria monocytogenes infection in mice. | mice treated with a contraceptive dose of beta-estradiol (e2) demonstrated changes in their macrophage (mphi) number and functions. while e2 increased and decreased the mphi number in pbmc and pec respectively, it enhanced the in vitro phagocytosis of fitc-labeled beads by both cells. e2 treatment also enhanced the phagocytic function of mphi as assessed by the in vivo carbon clearance assay. in contrast, the in vitro intracellular killing function of adherent cells in peritoneal exudate cells ( ... | 1999 | 10458538 |
| typing listeria monocytogenes by random amplified polymorphic dna (rapd) fingerprinting. | twenty epidemiologically unrelated listeria monocytogenes strains isolated from different animals, locations and on different dates in japan were classified into 18 types by the random amplified polymorphic dna (rapd) fingerprinting technique with four primers. further, seven epidemiologically related l. monocytogenes strains isolated from raw milk and a bulk tank on a dairy farm represented the same rapd type suggesting that they were all of the same origin. therefore, rapd-polymerase chain rea ... | 1999 | 10458115 |
| use of recombinant viruses to deliver cytokines influencing the course of experimental bacterial infection. | the feasibility of using viral constructs expressing cytokine genes to influence the course of bacterial infection was tested in mice. the mice were first infected with vaccinia or fowlpox viruses expressing the cytokine of interest, then challenged with the facultative intracellular bacterial pathogen listeria monocytogenes. the course of infection was assessed by subsequent bacterial counts. expression of ifn-gamma or tnf was protective. vaccinia virus was more efficient at delivering ifn-gamm ... | 1999 | 10457199 |
| disinfection of mung bean seed with gaseous acetic acid. | mung bean seed inoculated with salmonella typhimurium, escherichia coli o157:h7, and listeria monocytogenes (3 to 5 log cfu/g) was exposed to gaseous acetic acid in an aluminum fumigation chamber. salmonella typhimurium and e. coli o157:h7 were not detected by enrichment of seeds treated with 242 microl of acetic acid per liter of air for 12 h at 45 degrees c. l. monocytogenes was recovered by enrichment from two of 10 25-g seed samples treated in this manner. fumigation with gaseous acetic acid ... | 1999 | 10456753 |
| inhibition of food-related pathogenic bacteria by god-transformed penicillium nalgiovense strains. | two strains of penicillium nalgiovense, which carried the god gene of aspergillus niger and had increased glucose oxidase (god) activity compared with the wild-type strain, were tested for their ability to suppress the growth of certain food-related pathogenic bacteria. in contrast to the wild type, which showed no antibacterial effect when grown in mixed culture with different bacteria, the two transformed strains were highly antagonistic. the strain that expressed higher amounts of god in gene ... | 1999 | 10456750 |
| influence of guar gum on the thermal stability of listeria innocua, listeria monocytogenes, and gamma-glutamyl transpeptidase during high-temperature short-time pasteurization of bovine milk. | mathematical models describing the thermal inactivation of gamma-glutamyl transpeptidase (tp) and listeria innocua in milk during high-temperature short-time (htst) pasteurization were validated with data from tp, l. innocua, and l. monocytogenes trials in guar gum-milk. holding tube times were determined for turbulent flow using water, and for laminar flow using a guar gum (0.27% wt/wt)-sucrose (5.3% wt/wt)-water mixture. inactivation of tp and l. innocua was lower in a solution of guar gum (0. ... | 1999 | 10456737 |
| inactivation of escherichia coli o157:h7 and listeria monocytogenes on plastic kitchen cutting boards by electrolyzed oxidizing water. | one milliliter of culture containing a five-strain mixture of escherichia coli o157:h7 (approximately 10(10) cfu) was inoculated on a 100-cm2 area marked on unscarred cutting boards. following inoculation, the boards were air-dried under a laminar flow hood for 1 h, immersed in 2 liters of electrolyzed oxidizing water or sterile deionized water at 23 degrees c or 35 degrees c for 10 or 20 min; 45 degrees c for 5 or 10 min; or 55 degrees c for 5 min. after each temperature-time combination, the s ... | 1999 | 10456736 |